BLAST of luciferin 4-monooxygenase-like vs. L. salmonis genes
Match: EMLSAG00000011903 (supercontig:LSalAtl2s:LSalAtl2s83:250927:254806:1 gene:EMLSAG00000011903 transcript:EMLSAT00000011903 description:"maker-LSalAtl2s83-augustus-gene-2.4")

HSP 1 Score: 169.859 bits (429), Expect = 3.967e-45
Identity = 122/428 (28.50%), Postives = 210/428 (49.07%), Query Frame = 0

Query:  414 DLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMLKGIQHSFRINANIINRGYGPLSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQI---SKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKG-TRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKL-PGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRR 836
            ++  E +  ++QE+ AKFV+C      +V+    DL   I+ +       D  +++  +               D+     I+W+SGTTG  KGIQ +     N++     P +   +L +  F H  GF +     S     C F   + E+     +G  K+I   + +     +++ + H + + +     +++   + D V+ ICP  ++      + L  +F  L+NV NLY++TE G LI  S   R  G V     V I D  + E LG N  GE++ +   +  +GYLNRP ET+     DG+  +GD+ HY+++G +F++GR+KE++K  G H+YP+EIE II+ +   + E  +F K + I Q ++  +VV  +G  ++   I   V   +DDYK +   ++    LPRN  GK++R 
Sbjct:  131 NISTETLLSEVQETQAKFVICTNRTYYKVK----DLSSIIINL-------DTQLHAILEIDMSTQTQIPEQIEFDLQTLAVILWTSGTTGKPKGIQLNMVSLWNLL--FLSPKTDGNMLITCNFAHVSGFNYS-CFWSINFDVCTFHFISPEDVTS--LGVEKIIFTLS-QIPFTTIVVSASHIVYIAEKIVNDQIEYSSNFDEVKNICPAGAHTYPGISDDLRKLFRNLENVPNLYAMTESGGLILISKDQRSNGGVVGNMEVYIRDIHSGERLGPNTSGELIYRNPDKPTLGYLNRPDETKKFFLDDGWCCSGDMFHYNDHGELFYEGRIKEMLKSNGSHVYPIEIETIIQSMNSNIIEAGLFSKTEKI-QTILGGIVVIKKGYRIDPEEIRQFVNDRIDDYKHLEIIILTNEPLPRNPNGKLLRN 540          

HSP 2 Score: 122.865 bits (307), Expect = 1.800e-29
Identity = 113/431 (26.22%), Postives = 197/431 (45.71%), Query Frame = 0

Query:    5 KDKVLYSKMPEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCDLG-LRGLTEDEVGLDCRMGSEMEPMSRKSAKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMLKGIQHSFRIIANIINRGYGPLSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQI---SKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGXRDPDLKPELMRYQIQESGAKF 431
            ++K+L S+   D+S++ +   +++       +++ +  N + +FL+NC      G T  E      +   +E +  K AK L   G    DV+H+++ N  + + PV  TLLCQGVVS+   ++  E +  ++QE+ AKFV+C      +V    +DL   I+ +       D  +++  +               D+     I+W+SGTTG  KGIQ +   + N++     P +   +L +  F H  GF +     S     C F   + E+     +G  K+I   + +     +++ + H + + +     +++   + D V+ ICP  ++      + L  +F  L+NV NLY++TE G LI  S   R  G V     V I D  + E LG N  GE++    R+PD KP L      +   KF
Sbjct:   11 ENKILTSQNDFDDSVVLE-YNQNITSYFIDSMKKYMTKNSNKEFLVNCGKKYFPGETTSEGPESVSILDFIEKVP-KFAKYLYENGLRKGDVVHIIIGNRGDVYIPVHATLLCQGVVSISVDNISTETLLSEVQETQAKFVICTNRTYYKV----KDLSSIIINL-------DTQLHAILEIDMSTQTQIPEQIEFDLQTLAVILWTSGTTGKPKGIQLNMVSLWNLL--FLSPKTDGNMLITCNFAHVSGFNYS-CFWSINFDVCTFHFISPEDVTS--LGVEKIIFTLS-QIPFTTIVVSASHIVYIAEKIVNDQIEYSSNFDEVKNICPAGAHTYPGISDDLRKLFRNLENVPNLYAMTESGGLILISKDQRSNGGVVGNMEVYIRDIHSGERLGPNTSGELI---YRNPD-KPTLGYLNRPDETKKF 418          

BLAST of luciferin 4-monooxygenase-like vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 157.147 bits (396), Expect = 1.215e-40
Identity = 139/453 (30.68%), Postives = 216/453 (47.68%), Query Frame = 0

Query:  412 DPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDL--MVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDIDQR---MAIIWSSGTTGMLKGIQ--HSFRINANI-------INRGYGPLSAQTI-LQSTCFFHAGGFGFPISVLSG---GQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPR------HLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKT 840
            +P  +PE +  Q+  SGAK+V+     L  ++++      + KI+V+    EE  +   SF  ++A  DD  F      ID     +A+ +SSGTTG  KG+   HS  +N NI       I+   G    Q I +    FFH   F    S+ +G   G      P +  E           + +++  +Y+P  M++       +     +   L L S++ +   AS       E+      +       Y +TE   +      P+        G++ P T  KI+D DT + LG  + GE+   G +IM GY    K T+  +  +G+ HTGD+ +Y+++G  +   RLKELIK +G  + P E+E++IRK PGV +VA  G PD  N EL  A VVR   S V E  I++ VT+ V  +K++  GV+FV +LP+N  GKI+RR  K 
Sbjct:  143 NPTYRPEEIARQLDNSGAKYVMTIGLFLQNIKQSCEIYSGIQKIIVIGM--EETPSDCKSF-MEMAIFDDGSFFDQRETIDPHKDILALPYSSGTTGPPKGVALTHSNLVN-NITQLSHPSIHNFIGDEVEQHISIAVLPFFHI--FAMTCSLSAGLRFGVKLITLPKFEPE-----------MFINSLVQYKPTHMLVVPPLVSFMTSSPLIKPSL-LSSIKMLNGGASTFGPTLIEKFMNKCDSDLRFQEGYGMTECSPVT--HVQPKDGIIYGSCGNLIPNTIGKIIDIDTGKVLGPGENGELCVSGPQIMQGYYKNKKATDKIIQ-NGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYVVRKNRS-VMEQGIIDFVTEKVAPHKKLGAGVMFVDTLPKNQTGKILRRELKA 573          

HSP 2 Score: 60.077 bits (144), Expect = 1.913e-9
Identity = 89/392 (22.70%), Postives = 161/392 (41.07%), Query Frame = 0

Query:   77 MSRKSAKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDL--MVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDIDQR---MAIIWSSGTTGMLKGIQHSFRIIANIINRGYGP---------LSAQTILQSTCFFHAGGFGFPISVLSG---GQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPR------HLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGXRDPDLKPELMR--YQIQESGAKFV---VCHPGNLA 440
            +S+K    L  +G +  DV+ LV+PN  E+    +G       ++  +P  +PE +  Q+  SGAK+V+     L  ++++      + KI+V+    EE  +   SF  ++A  DD  F      ID     +A+ +SSGTTG  KG+  +   + N I +   P         +     +    FFH   F    S+ +G   G      P +  E           + +++  +Y+P  M++       +     +   L L S++ +   AS       E+      +       Y +TE   +      P+        G++ P T  KI+D DT + LG  + GE+   G       P++M+  Y+ +++  K +     H G++A
Sbjct:   96 LSQKFGSALLRLGAKKGDVMGLVVPNIPEFVIAFMGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMTIGLFLQNIKQSCEIYSGIQKIIVIGM--EETPSDCKSF-MEMAIFDDGSFFDQRETIDPHKDILALPYSSGTTGPPKGVALTHSNLVNNITQLSHPSIHNFIGDEVEQHISIAVLPFFHI--FAMTCSLSAGLRFGVKLITLPKFEPE-----------MFINSLVQYKPTHMLVVPPLVSFMTSSPLIKPSL-LSSIKMLNGGASTFGPTLIEKFMNKCDSDLRFQEGYGMTECSPVT--HVQPKDGIIYGSCGNLIPNTIGKIIDIDTGKVLGPGENGELCVSG-------PQIMQGYYKNKKATDKIIQNGWXHTGDIA 461          

BLAST of luciferin 4-monooxygenase-like vs. L. salmonis genes
Match: EMLSAG00000010401 (supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 gene:EMLSAG00000010401 transcript:EMLSAT00000010401 description:"maker-LSalAtl2s68-augustus-gene-3.30")

HSP 1 Score: 130.183 bits (326), Expect = 4.833e-32
Identity = 76/211 (36.02%), Postives = 114/211 (54.03%), Query Frame = 0

Query:  636 VFPALKN-VFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIM-AKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKL-PGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPS-LPRNSIGKIMRRHAKTMY 842
            +F  LK  V N Y  TE G     ST     G V   T   I D +T + LG N++GE+M  K      GYLNR +E E   + DG+ HTGD  HY E G  FF  R+ E+IK +G H+YP+E+E +++ +   + EV V    +   Q ++ A++V      +N  TI   + + VD+YKRI G ++     LPRN++GK++R   + ++
Sbjct:  278 LFEVLKKCVPNFYGSTESGGGFLVSTNQHSNGGVVARTETYIRDLNTGQHLGPNKIGELMFRKANATSTGYLNRSEENEKMFEKDGWTHTGDFFHYTEQGEFFFHSRINEMIKCQGSHVYPIELEGLLKAMHDDIIEVGVVPVVEK-KQTVLGAIIVVKNDKKINVETIRKELNKKVDEYKRIEGQLLLTKEPLPRNAMGKLLRSEIQKLF 487          

HSP 2 Score: 81.2629 bits (199), Expect = 3.201e-16
Identity = 63/262 (24.05%), Postives = 114/262 (43.51%), Query Frame = 0

Query:    5 KDKVLYSKMPEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCDLGLRGLTEDEVGLDCRMGSEMEPMSRKSAKLLRSM---GFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKA-----TRDLMVKILVVRDCPEE---PDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMLKGIQHSFRIIANIINRGYGPLSAQTILQSTCFFHAGGF 255
            +D VL +K  +DN  +     ++L E    K+ + +E N D + L+NC    +    DE   D  +   ++    K   L +S+   G    DV+HL++ N  + + PV  T+LCQGV+S+    +  + +  ++ ++ A+ V C      +V+         D  ++ ++   C  +   P   V+S                   +     I+WSSGTTG  KG+Q +  +++ + +    P     +L S  F H  GF
Sbjct:   11 EDYVLTTK-SDDNRTLQAEYNQNLAECFLDKITKYLEVNEDKELLVNCG---KRFFPDETVSDGPVAHTLKDFLHKVPALAKSLYEKGLRKSDVVHLIIGNRADIYLPVFATILCQGVISISVDSISHDTLMTELNDTQAEIVFCTNKTFKKVKNCGCTIINMDTDIEEMIANGCTSKVIVPKQDVFS-------------------LKDVFVILWSSGTTGQPKGVQMT--VVSFLDSMFATPKVKGNMLLSANFGHISGF 247          

BLAST of luciferin 4-monooxygenase-like vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 122.865 bits (307), Expect = 3.131e-29
Identity = 121/460 (26.30%), Postives = 197/460 (42.83%), Query Frame = 0

Query:  414 DLKPELMRYQIQESGAKFVVCHPGNLAQ---VRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIW------SSGTTGML---KGIQHSFRINANIINRGYGPLSAQTILQSTCFFHAGGF-GFPISVLSGGQTACFFPTYTL-ENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICP----TASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVD--------------------SDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMR 835
            +L P+ +  Q++ES  K +VC   N        + T  +   I++  +C  +  + V      L   DD + V   +  D    +I+      SSG  G+L   K +   F       N  +  L   +I  +  FFH  GF  F +  + G         YTL E +EQ         L+A    +   +   +   I++   S + S  DL S++ +       +S I  +  +R+    P++K V   Y L E G L+T +       S+A      + D                     D     G +Q GEI  K  + M+GYLN  KE  +  D+D + HTGDLG+YD  GI++F  ++  LI+F    + P  +E  +     + + AV G P+  N ++  A VV   G    E  + NL+   + D++RIRGG+ FV ++PR+   K++R
Sbjct:  145 ELPPDELELQMRESKTKILVCDDFNYVDGLDFLENTNMIEKLIIIGNECTAQ--DKVILMTDLLN--DDGKKVPQKVIFDWAKDVIYMPFTSTSSGAKGILHTNKSLMSQFYSPDGAANHWFDQLIGDSIHCANYFFHMTGFYNFALGSIYG------ISIYTLSEFSEQNF-------LEALVDXKTASISCYAWQ-IRILSQSPLVSNYDLSSLKVLMTGCGLLSSTIRMEVLDRI----PSIKYVREAYGLNECG-LVTLTYPREKKNSIASSKAADVPDDHIIPMGLPNMYSMIKIISRQDGNPVDGPDQQGEICVKSPQCMLGYLN--KEESSAFDNDQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTNLMESRLQDHERIRGGLYFVQNIPRDENWKVLR 579          

HSP 2 Score: 55.0694 bits (131), Expect = 6.188e-8
Identity = 84/340 (24.71%), Postives = 135/340 (39.71%), Query Frame = 0

Query:   69 RMGSEMEPMSRKSAKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLA---QVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIW------SSGTTGML---KGIQHSFRIIANIINRGYGPLSAQTILQSTCFFHAGGF-GFPISVLSGGQTACFFPTYTL-ENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICP----TASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVD 390
            R G+ +  +  K A  L   GF   DVL L   N  EY+   LG   C G+V   + +L P+ +  Q++ES  K +VC   N        + T  +   I++  +C  +  + V      L   DD + V   +  D    +I+      SSG  G+L   K +   F       N  +  L   +I  +  FFH  GF  F +  + G         YTL E +EQ         L+A    +   +   +   I++   S + S  DL S++ +       +S I  +  +R+    P++K V   Y L E G L+T +       S+A      + D
Sbjct:   89 RFGN-VHSLVNKVASGLTKNGFVKGDVLTLYCSNFVEYWILALGVWTCGGIVMPVNCELPPDELELQMRESKTKILVCDDFNYVDGLDFLENTNMIEKLIIIGNECTAQ--DKVILMTDLLN--DDGKKVPQKVIFDWAKDVIYMPFTSTSSGAKGILHTNKSLMSQFYSPDGAANHWFDQLIGDSIHCANYFFHMTGFYNFALGSIYG------ISIYTLSEFSEQNF-------LEALVDXKTASISCYAWQ-IRILSQSPLVSNYDLSSLKVLMTGCGLLSSTIRMEVLDRI----PSIKYVREAYGLNECG-LVTLTYPREKKNSIASSKAADVPD 404          

BLAST of luciferin 4-monooxygenase-like vs. L. salmonis genes
Match: EMLSAG00000007696 (supercontig:LSalAtl2s:LSalAtl2s448:237586:242919:1 gene:EMLSAG00000007696 transcript:EMLSAT00000007696 description:"maker-LSalAtl2s448-augustus-gene-2.14")

HSP 1 Score: 120.553 bits (301), Expect = 1.201e-28
Identity = 101/369 (27.37%), Postives = 171/369 (46.34%), Query Frame = 0

Query:  488 DIDQRMAIIWSSGTTGMLKGIQHSFRINANIINR----GYGPLSAQTILQS-----------TCFFHAGGFGFPI-SVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISK-VDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEII-RKLPGVSEVAVFGKPDPINQELVTAMVVR---AEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMR 835
            D ++ + I    GTT +   I        NI+N      Y P     ++++           T FFH GGF   + ++L   +T        L +  + +  ++  + +A    +P  ++     A+++   S  + SQ DL SV  I P   +I  +  ++    FP+LK +   Y  TE  + I+   + +HLG + P   ++I++ +  E LG N VG+I+ K    M  Y+    + E  +  DGF + GD+G+YD  G +FF  R K+L+K    H+YP EIE  I  K   V ++ VFG+ D    E ++ ++V     +G ++               YK ++GGV    ++PRN+ GKI R
Sbjct:  188 DDNEVVEIFNREGTTSLQSEIAGVLYSAENILNSIHYCSYVPFQIHHVIKNYEPSSLRLMILTNFFHPGGFTEGLRNILPQKRTGII-----LRSPNEYVDFSTTHLFNAIQTTKPNMIICSPEFAVKMNGASNDLSSQFDLSSVFVIYPIGGSINTNYEKKARNQFPSLKMICKGYGSTETLS-ISLGISDQHLGRLFPKYQLRILNPNG-EPLGPNSVGDIVVKPGYGMRRYIG---DHEYNITQDGFFYMGDVGYYDSAGKIFFKCRSKDLVKVHNHHVYPQEIESFISSKCSFVKDIGVFGQKDKNGIERLSGVIVLHHIEKGLSLQNVLERTFEAYKFPRYKWLQGGVRVTSAIPRNANGKIFR 546          

HSP 2 Score: 63.929 bits (154), Expect = 1.148e-10
Identity = 81/332 (24.40%), Postives = 143/332 (43.07%), Query Frame = 0

Query:   98 LVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQI----QESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGM---LKGIQHSFRIIANIINR-GYGPLSAQTILQS-----------TCFFHAGGFGFPI-SVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISK-VDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAK 408
            L+L NT  +F PV+GT      VSL  P    E +  Q+    +E   + +V      A + K +   + +I+ +   P       Y   Q++   DD + V           I    GTT +   + G+ +S   I N I+   Y P     ++++           T FFH GGF   + ++L   +T        L +  + +  ++  + +A    +P  ++     A+++   S  + SQ DL SV  I P   +I  +  ++    FP+LK +   Y  TE  + I+   + +HLG + P   ++I++ +  E LG N VG+I+ K
Sbjct:   99 LLLNNTRFFFIPVVGTWFLGSSVSLFQPSSSRETIERQLMILKEELNLEEIVVITETFA-IPKISNIGVDRIISLTPVPG------YMSLQEIFMNDDNEVVE----------IFNREGTTSLQSEIAGVLYSAENILNSIHYCSYVPFQIHHVIKNYEPSSLRLMILTNFFHPGGFTEGLRNILPQKRTGII-----LRSPNEYVDFSTTHLFNAIQTTKPNMIICSPEFAVKMNGASNDLSSQFDLSSVFVIYPIGGSINTNYEKKARNQFPSLKMICKGYGSTETLS-ISLGISDQHLGRLFPKYQLRILNPNG-EPLGPNSVGDIVVK 406          

BLAST of luciferin 4-monooxygenase-like vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 83.1889 bits (204), Expect = 1.962e-16
Identity = 60/210 (28.57%), Postives = 100/210 (47.62%), Query Frame = 0

Query:  640 LKNVFNLYSLTEFGALITFSTTPR-----HLGSVAPG---TTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKTM 841
            +K++ N+Y  TE G  ++F   P+      + +V P       KIVD + +  L  N  GE+  +   +M GY    K ++  +  D + H+GDLG   E G     GRL +++   G++IYP EIE+++ K P + EV + G PD    E + A +   EG+   E  +       +  +K I    +FV   P+   GK+ +   K +
Sbjct:  336 MKSLMNIYGATETGP-VSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGK-ILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEELCAWICLEEGA--KEFDLKEHCKGKISHFK-IPKYPMFVKDFPKTLSGKVKKFEMKAI 540          

HSP 2 Score: 82.0333 bits (201), Expect = 4.266e-16
Identity = 61/217 (28.11%), Postives = 100/217 (46.08%), Query Frame = 0

Query:  636 VFPALKNVFNLYSLTEFGALITFSTTPR-----HLGSVAPG---TTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGS---NVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKTM 841
            V  A K + N+Y  TE G  ++F   P+      + +V P       KIVD + +  L  N  GE+  +   +M GY    K ++  +  D + H+GDLG   E G     GRL +++   G++IYP EIE+ + K P + EV + G PD    E + A +   EG+   ++ E+    +    +  Y       +FV   P+   GK+ +   K +
Sbjct:  104 VLEAQKKLRNIYGATETGP-VSFIACPKDSFEVRINTVGPPLGHIEAKIVDQNGK-ILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEELCAWICLEEGAKEFDLKEHCKGKIAHFKIPKYP------MFVKDFPKTLSGKVKKFEMKAI 312          

HSP 3 Score: 80.8777 bits (198), Expect = 1.002e-15
Identity = 66/242 (27.27%), Postives = 111/242 (45.87%), Query Frame = 0

Query:  609 QLDLDSVQYICPTASN----IPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPR-----HLGSVAPG---TTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGS---NVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMR 835
            ++D+ SV+      SN    + ED  + L+     +K++ N+Y  TE G   TF  +P       + +V P       KIVD + +  L  N  GE+  +   +M GY    K ++  +  D + H+GDL    E G     GRL +++   G++IYP EIE+ + K P + EV + G PD    E + A +   EG+   ++ E+    +    +  Y       +FV   P+   GK+ +
Sbjct:  677 KVDVSSVELGITGGSNCLVEVCEDFEKHLN-----MKSLMNIYGATETGP-ATFVPSPEDSFEVRINTVGPPLGHVEAKIVDQNGK-ILPVNTPGELWVRTPGLMHGYYKDQKSSQKAIGPDRYYHSGDLAMITEKGYGKIIGRLDDMVNRGGENIYPKEIEDFLLKHPNIVEVYIIGVPDDYMGEELCAWICLEEGAKEFDLKEHCKGKIAHFKIPKYS------MFVKDFPKTLSGKVKK 905          

BLAST of luciferin 4-monooxygenase-like vs. L. salmonis genes
Match: EMLSAG00000009904 (supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-1 gene:EMLSAG00000009904 transcript:EMLSAT00000009904 description:"maker-LSalAtl2s645-snap-gene-2.24")

HSP 1 Score: 66.6254 bits (161), Expect = 2.040e-11
Identity = 85/369 (23.04%), Postives = 155/369 (42.01%), Query Frame = 0

Query:  495 IIWSSGTTGMLKGIQHSFR-INANI--INRGYGPLSAQTILQSTCFFHAGGFGFPISV-LSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFG-----ALITFSTTPRHLGSVAPGTTVKIVD---------SDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDG-FLHTGDLGHYDENGIMFFDGRLK-ELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKTMYS 843
            I+++SGT+G  KG+  + R I A +  + + +   S   IL     +H  G    + V LS G      P +  +     L  NS + +           +L   + +     +++   L       +C +AS +PE    +   +   +  +   Y +TE G     +L      P  +G+  P    +I+          +D + +  +++ GE+  +G  +   Y    K T+   D +G +  TGD    +  G+    GR   ++IK  G  +  +EIE      P +SE+AV G  D I  E+V A++V  +G  +N + +     + +  Y   R   + V S+ +N +GKI ++  K M S
Sbjct:  152 ILYTSGTSGSPKGVWITHRNIEAQVSGMIKFWAWTSDDHILHVLPLYHLHGLINCLLVPLSIGARVKMLPGFDAKKVWSYLT-NSSMNISVFMGVPTIYSLLTKEYELDKRNKTEIHDMLRSTYRLMVCGSAS-LPEPLLRKWQTISGHI--LLERYGMTEIGMGLGNSLEEDKRLPGFVGTPFPNVEARIMSEEGSVLAYSNDVEFSYLSDKSGELQIRGPSVFSFYYGNEKATKESFDPNGDWFLTGDTASMNSRGVFKILGRTSADIIKSGGFKLSALEIEREYLSYPDISEIAVVGLEDEIFGEIVAAIIVPNKGYYLNSDILRGFGYKKLPPYSVPR-QFMLVNSIDKNHMGKINKKELKKMDS 515          

BLAST of luciferin 4-monooxygenase-like vs. L. salmonis genes
Match: EMLSAG00000003336 (supercontig:LSalAtl2s:LSalAtl2s182:521806:564306:1 gene:EMLSAG00000003336 transcript:EMLSAT00000003336 description:"maker-LSalAtl2s182-augustus-gene-5.28")

HSP 1 Score: 57.7658 bits (138), Expect = 1.298e-8
Identity = 88/338 (26.04%), Postives = 129/338 (38.17%), Query Frame = 0

Query:  486 HLDIDQRMAIIWSSGTTGMLKGIQHSFRI---------------NANIINRGYGPLS--AQTILQSTCFFHAGG----------FGFPISVLSGGQTACFFPT-YTLENTEQGLVGNSK-----------LILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLD-SVQYICPTASNIPEDTYERLHGVFPALK-NVFNLYSLTEFGALITFSTTPRH---LGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFE-GKHIYPMEIEEIIRK-LPGVSEVAVFGKPD 777
            H+ ++Q  ++I++SGTTG  KG+  S  I                A  I   Y PLS  A + L      +  G           G  +  L   +   FF      E  ++G+   SK           +  DA+  Y   G     H         KV   L LD  +++I   A        ER    F +L   V   Y L+E G   +   +       G++ P T  K+V S + + +G    GEI  KG  +MMGYL   + T    D +G+ HT               GRLK+L+    G+++ P  IE  I+  LP VS   V G  +
Sbjct:  212 HISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAP-----LSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFKTGTCGTIIPSTHAKVVKS-SDDDIG----GEICIKGRCVMMGYLFNEQATSEAFDKEGYFHT---------------GRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAE 524          

BLAST of luciferin 4-monooxygenase-like vs. L. salmonis genes
Match: EMLSAG00000004507 (supercontig:LSalAtl2s:LSalAtl2s235:163315:165883:-1 gene:EMLSAG00000004507 transcript:EMLSAT00000004507 description:"maker-LSalAtl2s235-augustus-gene-1.27")

HSP 1 Score: 55.0694 bits (131), Expect = 5.629e-8
Identity = 37/121 (30.58%), Postives = 56/121 (46.28%), Query Frame = 0

Query:  719 GFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRA---EGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRR 836
            G+  TGD  +  + G +   GR+ ++I   G  +   E+E+++ K   + E AV G P  I  E + A VV     EG    E  I  LV  ++  +  +     F   LP+   GKIMRR
Sbjct:  442 GYYFTGDGAYRTKEGYISIVGRIDDVINVSGHRLGTAEVEDVLTKHDNIVEAAVVGXPHDIKGEAIYAFVVPKNLDEGVKKFEKGIQKLVKDNISGFA-VPEKFQFCEDLPKTRSGKIMRR 561          

BLAST of luciferin 4-monooxygenase-like vs. SwissProt
Match: gi|126501|sp|P08659.1|LUCI_PHOPY (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 165.622 bits (418), Expect = 3.802e-42
Identity = 116/378 (30.69%), Postives = 183/378 (48.41%), Query Frame = 0

Query:  478 DDAQFVSDHLDIDQRMAIIW-SSGTTGMLKGIQHSFRINANIINRGYGPLSAQTILQSTCF-----FHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYIC----PTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST----TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKTM 841
            ++  FV +  D D+ +A+I  SSG+TG+ KG+    R      +    P+    I+  T       FH G FG   ++   G   C F    +   E+      +L L +   Y+ +  +L         + + +D + DL ++  I     P +  + E   +R H     L  +   Y LTE  + I  +      P  +G V P    K+VD DT +TLG NQ GE+  +G  IM GY+N P+ T A +D DG+LH+GD+ ++DE+   F   RLK LIK++G  + P E+E I+ + P + +  V G PD    EL  A+VV   G  + E  I++ V   V   K++RGGV+FV  +P+   GK+  R  + +
Sbjct:  177 NEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHG-FGMFTTL---GYLICGFRVVLMYRFEE------ELFLRSLQDYKIQSALLVPTLFSFFAKSTLID-KYDLSNLHEIASGGAPLSKEVGEAVAKRFH-----LPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREI 538          

HSP 2 Score: 65.855 bits (159), Expect = 8.001e-10
Identity = 81/357 (22.69%), Postives = 145/357 (40.62%), Query Frame = 0

Query:   72 SEMEPMSRKSAKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDL-MVKILVVRDCPEEPD--NHVYSFCQQ--LAKVDDAQFVSDHLDIDQRMAIIW-SSGTTGMLKGIQHSFRIIANIINRGYGPLSAQTILQSTCF-----FHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYIC----PTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST----TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKG 409
            +E   MS + A+ ++  G      + +   N+ ++F PVLG L     V+  +       +   +  S    V      L ++    + L +++ +++ D   +      +Y+F         ++  FV +  D D+ +A+I  SSG+TG+ KG+    R      +    P+    I+  T       FH G FG   ++   G   C F    +   E+      +L L +   Y+ +  +L         + + +D + DL ++  I     P +  + E   +R H     L  +   Y LTE  + I  +      P  +G V P    K+VD DT +TLG NQ GE+  +G
Sbjct:   54 AEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHG-FGMFTTL---GYLICGFRVVLMYRFEE------ELFLRSLQDYKIQSALLVPTLFSFFAKSTLID-KYDLSNLHEIASGGAPLSKEVGEAVAKRFH-----LPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRG 394          

BLAST of luciferin 4-monooxygenase-like vs. SwissProt
Match: gi|158564046|sp|Q84P21.2|4CLL5_ARATH (RecName: Full=4-coumarate--CoA ligase-like 5; AltName: Full=4-coumarate--CoA ligase isoform 9; Short=At4CL9; AltName: Full=Peroxisomal OPC-8:0-CoA ligase 1)

HSP 1 Score: 164.851 bits (416), Expect = 6.357e-42
Identity = 122/426 (28.64%), Postives = 201/426 (47.18%), Query Frame = 0

Query:  423 QIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMLKGIQHSFR----INANIINRGYGPLSAQTILQSTCFFHAGGFG-FPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQ-ISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTE---FGALITFSTTPRHLGS---VAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRR 836
            QI++S           L ++  A + L   I+++ +   +    V    + + K      V + +D D    +++SSGTTGM KG+  S R    +   I+NR       Q  + +   FH  G   F   +L+ G T      + +             ++ A  +Y+   + L     + +     ++ ++ DL S+  +    + + ++  E     +P +K +   Y LTE    GA        R  G+   ++     +IVD  T + LG  Q GE+  KG  IM GY +  + T + LDS+G+L TGDL + DE+G +F   RLKELIK++G  + P E+E ++   P +++ AV   PD    +   A VVR  GS+++E TI+  V + V  YKRIR  V FV S+P+N  GKI+R+
Sbjct:  124 QIKDSNPVLAFTTSQLLPKISAAAKKL--PIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHE-----------MMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVK-ILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIR-KVAFVSSIPKNPSGKILRK 534          

HSP 2 Score: 62.003 bits (149), Expect = 1.272e-8
Identity = 78/346 (22.54%), Postives = 139/346 (40.17%), Query Frame = 0

Query:   82 AKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLG------DPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMLKGIQHSFR----IIANIINRGYGPLSAQTILQSTCFFHAGGF-GFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQ-ISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTE---FGALITFSTTPRHLGS---VAPGTTVKIVDSDTQETLGANQVGEIMAKG 409
            A  L  +G     V+ L+ PN+    FPV+    C  V+SLG      +P      +  QI++S           L ++  A + L   I+++ +   +    V    + + K      V + +D D    +++SSGTTGM KG+  S R    ++  I+NR       Q  + +   FH  G   F   +L+ G T      + +             ++ A  +Y+   + L     + +     ++ ++ DL S+  +    + + ++  E     +P +K +   Y LTE    GA        R  G+   ++     +IVD  T + LG  Q GE+  KG
Sbjct:   71 ADCLSEIGIRKGHVVLLLSPNSI--LFPVV----CLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKL--PIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHE-----------MMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVK-ILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKG 396          

BLAST of luciferin 4-monooxygenase-like vs. SwissProt
Match: gi|209572802|sp|Q8RU95.2|4CLL6_ORYSJ (RecName: Full=4-coumarate--CoA ligase-like 6)

HSP 1 Score: 165.236 bits (417), Expect = 9.361e-42
Identity = 115/373 (30.83%), Postives = 180/373 (48.26%), Query Frame = 0

Query:  494 AIIWSSGTTGMLKGIQHSFR-----INA-NII----------NRGYGPLS-------AQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRH------LGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRH 837
            A+++SSGTTG +K +  + R     I+A N I          + G G          A   L     FH  GFG     +S G+TA     + L    +           A  RYR   +       + L + S    + DL S+  I    + +  +  +R   VFP+++ +   Y LTE    +     P        +G +AP    KIVD+ T E LG  + GE+  +G  +M GY+  P+ T A +  DG+L TGDL +++E+G ++   RLKELIK++G  + P E+E I++  P +++ AV   PD    +L  A VVR  G+ + E  ++N V +HV  YK++R  V FV ++P++  GKI+RR 
Sbjct:  228 ALLYSSGTTGRVKAVAITHRNLIAQISAYNAIRETVAREAATDAGKGKPPPPSPSPPAAVTLFPLPLFHVMGFGLLTRTISSGETAVVMRRFDLAAAAR-----------AVERYRVTKLSAAPPVVVALTK-SDEARRRDLSSLVAIVVGGAPLGREVSQRFATVFPSVQ-IVQSYGLTESTGPVATMAGPEESAAYGSVGRLAPRVQAKIVDTATGEVLGPGRRGELWIRGPVVMKGYVGDPEATAATITPDGWLKTGDLCYFNEDGYLYVVDRLKELIKYKGYQVPPAELEHILQSRPEIADAAVVPYPDEEAGQLPMAFVVRQPGAYLTEQQVMNCVAKHVAPYKKVR-RVAFVNAIPKSPAGKILRRE 586          

BLAST of luciferin 4-monooxygenase-like vs. SwissProt
Match: gi|158564047|sp|Q84P23.2|4CLL9_ARATH (RecName: Full=4-coumarate--CoA ligase-like 9; AltName: Full=4-coumarate--CoA ligase isoform 4; Short=At4CL4)

HSP 1 Score: 164.466 bits (415), Expect = 9.980e-42
Identity = 116/387 (29.97%), Postives = 187/387 (48.32%), Query Frame = 0

Query:  470 FCQQLAKVDDAQFVSDHLDIDQR--MAIIWSSGTTGMLKGIQHSFRINANIINRGYGPLSAQTILQ-----------STCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHL------GSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRH 837
            F   L + D +      + ++Q    AI++SSGTTG +KG+  + R   N+I      +S Q  LQ           S   FH  GF   I  +S G+T      + LE            +  A  +Y+  GM +     + L + S++  + DL S++ +    + + +D  ER    FP + ++   Y LTE       +  P  +      G ++     KIVD  T E+L   + GE+  +G  IM GY+   K +   +D +G+L TGDL ++D    ++   RLKELIK++   + P+E+E+I+   P V + AV   PD    E+  A +VR  GSN+NE  I++ V + V  YK++R  V F+ ++P+N  GKI+RR 
Sbjct:  182 FLSWLNRSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHR---NLI--ASTAVSHQRTLQDPVNYDRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEA-----------MFKAVEKYKVTGMPVSPPLIVALVK-SELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDV-DIVQGYGLTESSGPAASTFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGYVGNEKASAETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSNPDVIDAAVVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVR-RVAFINAIPKNPAGKILRRE 549          

HSP 2 Score: 59.6918 bits (143), Expect = 6.360e-8
Identity = 62/247 (25.10%), Postives = 103/247 (41.70%), Query Frame = 0

Query:  182 FCQQLAKVDDAQFVSDHLDIDQR--MAIIWSSGTTGMLKGIQHSFRIIANIINRGYGPLSAQTILQ-----------STCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHL------GSVAPGTTVKIVDSDTQETLGANQVGEIMAKG 409
            F   L + D +      + ++Q    AI++SSGTTG +KG+  + R   N+I      +S Q  LQ           S   FH  GF   I  +S G+T      + LE            +  A  +Y+  GM +     + L + S++  + DL S++ +    + + +D  ER    FP + ++   Y LTE       +  P  +      G ++     KIVD  T E+L   + GE+  +G
Sbjct:  182 FLSWLNRSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHR---NLI--ASTAVSHQRTLQDPVNYDRVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEA-----------MFKAVEKYKVTGMPVSPPLIVALVK-SELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDV-DIVQGYGLTESSGPAASTFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRG 410          

BLAST of luciferin 4-monooxygenase-like vs. SwissProt
Match: gi|126500|sp|P13129.1|LUCI_LUCCR (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 161.77 bits (408), Expect = 7.388e-41
Identity = 112/372 (30.11%), Postives = 186/372 (50.00%), Query Frame = 0

Query:  480 AQFVSDHLDIDQRMAIIW-SSGTTGMLKGIQHSFRINANIINRGYGPL-----SAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST----TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKTM 841
            + F +  +D  +++A+I  SSG+TG+ KG+Q +        +    P+     S  T + +   FH G FG   ++   G   C F    L   ++      +  L     Y+   ++L       +   S++ ++ DL ++  I    + + ++  E +   F  L  V   Y LTE  + I  +      P   G V P    K++D DT+++LG N+ GE+  KG  +M GY+N P+ T+  +D +G+LHTGD+G+YDE    F   RLK LIK++G  + P E+E ++ + P + +  V G PDP+  EL  A+VV   G N+ E  +++ V   V + KR+RGGV FV  +P+   GKI  R  + +
Sbjct:  181 SSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPGTAVLTVVPFHHG-FGMFTTL---GYLICGFRVVMLTKFDE------ETFLKTLQDYKCTSVIL-VPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRF-NLPGVRQGYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKIDGRAIREI 540          

BLAST of luciferin 4-monooxygenase-like vs. SwissProt
Match: gi|158564049|sp|Q84P25.2|4CLL2_ARATH (RecName: Full=4-coumarate--CoA ligase-like 2)

HSP 1 Score: 161.77 bits (408), Expect = 9.309e-41
Identity = 113/365 (30.96%), Postives = 180/365 (49.32%), Query Frame = 0

Query:  483 VSDHLDIDQRMAIIWSSGTTGMLKGIQHSFRINANIIN--RGYGPLSAQTILQSTCFFHAGGFG-FPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQ-ISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGA----LITFSTTPRH--LGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRH 837
            V   ++ D   A+++SSGTTG  KG+  S R    ++   R    L  +TI  +    H  GFG F   +++ G T    P + +             +L A   +R   + L     + +    ++++S+ DL S+  +    + +  +  E+    +P +K +   Y LTE  A    +     T R+   G +AP    KIVD DT   LG NQ GE+  +   +M GY    + T + +DS+G+L TGDL + D +G +F   RLKELIK  G  + P E+E ++   P +++ AV   PD    +   A +VR  GSN++E+ I+  V + V  YK+IR  V F+ S+P+N  GKI+RR 
Sbjct:  206 VKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRTIC-TIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAK-----------LLSAVETHRSSYLSLVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVK-ILQGYGLTESTAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIR-KVTFLASIPKNPSGKILRRE 556          

HSP 2 Score: 62.003 bits (149), Expect = 1.285e-8
Identity = 76/347 (21.90%), Postives = 144/347 (41.50%), Query Frame = 0

Query:   80 KSAKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGA--KFVVCHPGNLAQVRKATRDLMVKILVVRD--CPEEPDNHVYSFCQQLAKVDDAQ----FVSDHLDIDQRMAIIWSSGTTGMLKGIQHSFRIIANIIN--RGYGPLSAQTILQSTCFFHAGGFG-FPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQ-ISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGA----LITFSTTPRH--LGSVAPGTTVKIVDSDTQETLGANQVGEIMAK 408
            + A  L ++G    +V+ ++ PN+  +    L  +    +++  +P    + +  QI +S     F  C    L     A  +  + ++++ D   P +          +L  + + +     V   ++ D   A+++SSGTTG  KG+  S R +  ++   R    L  +TI  +    H  GFG F   +++ G T    P + +             +L A   +R   + L     + +    ++++S+ DL S+  +    + +  +  E+    +P +K +   Y LTE  A    +     T R+   G +AP    KIVD DT   LG NQ GE+  +
Sbjct:   86 RVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIGDSRPVLAFTTC---KLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRTIC-TIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAK-----------LLSAVETHRSSYLSLVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVK-ILQGYGLTESTAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIR 416          

BLAST of luciferin 4-monooxygenase-like vs. SwissProt
Match: gi|12229631|sp|O24145.1|4CL1_TOBAC (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 160.614 bits (405), Expect = 2.060e-40
Identity = 136/472 (28.81%), Postives = 227/472 (48.09%), Query Frame = 0

Query:  397 LGANQVGEIMAKGXRDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMLKGIQHSFRINANIINRGYGPLSAQTI--LQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGL-----VGNSKLI---------LDATHRYRPEGMMLGSHHAIQLCQISK---VDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALIT----FSTTPRHLGSVAPGTTV-----KIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKT 840
            +GA+ +G I      +P   P  +  Q + S AK ++     + +V+    +  VK++ +   PE        F  +L + D+ +     +  D  +A+ +SSGTTG+ KG+         + ++G     AQ +    +  + H           S     C  P + + +    L     VG + LI         L+   +Y+   + +G      +  I+K   VDS  DL SV+ +   A+ + ++  + +   FP  K +   Y +TE G ++     F+  P  + S A GT V     KIVD DT  +L  NQ GEI  +G +IM GYLN P+ T   +D +G+LHTGD+G  DE+  +F   RLKELIK++G  + P EIE ++   P +S+ AV    D    E+  A VVR+ GS + E+ + + +++ V  YKR++  V FV ++P++  GKI+R+  + 
Sbjct:  100 MGASYLGAISTMA--NPLFTPAEVVKQAKASSAKIIITQSCFVGKVKDYASENDVKVICIDSAPE----GCLHF-SELTQSDEHEIPEVKIQPDDVVALPYSSGTTGLPKGVM--------LTHKGLVTSVAQQVDGENANLYMH-----------SEDVLMCVLPLFHIYSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYK---VSIGPFVPPIVLAIAKSPIVDS-YDLSSVRTVMSGAAPLGKELEDAVRTKFPNAK-LGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVK-RVFFVETVPKSPSGKILRKDLRA 539          

HSP 2 Score: 77.411 bits (189), Expect = 2.016e-13
Identity = 90/366 (24.59%), Postives = 159/366 (43.44%), Query Frame = 0

Query:   72 SEMEPMSRKSAKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDIDQRMAIIWSSGTTGMLKGIQHSFRIIANIINRGYGPLSAQTI--LQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGL-----VGNSKLI---------LDATHRYRPEGMMLGSHHAIQLCQISK---VDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGAL----ITFSTTPRHLGSVAPGTTV-----KIVDSDTQETLGANQVGEIMAKG 409
            +E+E   RK A  L  +G + +D + ++LPN+ E+ F  +G      + ++ +P   P  +  Q + S AK ++     + +V+    +  VK++ +   PE        F  +L + D+ +     +  D  +A+ +SSGTTG+ KG+         + ++G     AQ +    +  + H           S     C  P + + +    L     VG + LI         L+   +Y+   + +G      +  I+K   VDS  DL SV+ +   A+ + ++  + +   FP  K +   Y +TE G +    + F+  P  + S A GT V     KIVD DT  +L  NQ GEI  +G
Sbjct:   61 AEVELTCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKIIITQSCFVGKVKDYASENDVKVICIDSAPE----GCLHF-SELTQSDEHEIPEVKIQPDDVVALPYSSGTTGLPKGVM--------LTHKGLVTSVAQQVDGENANLYMH-----------SEDVLMCVLPLFHIYSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYK---VSIGPFVPPIVLAIAKSPIVDS-YDLSSVRTVMSGAAPLGKELEDAVRTKFPNAK-LGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRG 397          

BLAST of luciferin 4-monooxygenase-like vs. SwissProt
Match: gi|61213879|sp|Q26304.1|LUCI_LUCMI (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 157.918 bits (398), Expect = 1.317e-39
Identity = 113/357 (31.65%), Postives = 177/357 (49.58%), Query Frame = 0

Query:  491 QRMAIIW-SSGTTGMLKGIQHSFRINANIINRGYGPL-----SAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYIC----PTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST----TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKI 833
            Q +A++  SSG+TG+ KG++ +        +    P+     S  T + +   FH G FG   ++   G  AC +    L   ++      +L L     Y+   ++L       +   S++  + DL ++  I     P A  + E    R +     L  V   Y LTE  +    +      P   G V P   VK++D DT++TLG N+ GEI  KG  +M+GY N P+ T   +D +G+LHTGD+G+YDE+   F   RLK LIK++G  + P E+E ++ + P + +  V G PDP   EL  A+VV  +G  + E  I++ V   V ++KR+RGGV FV  +P+   GKI
Sbjct:  192 QHVALLMNSSGSTGLPKGVRITHEGAVTRFSHAKDPIYGNQVSPGTAILTVVPFHHG-FGMFTTL---GYFACGYRVVMLTKFDE------ELFLRTLQDYKCTSVIL-VPTLFAILNKSELIDKFDLSNLTEIASGGAPLAKEVGEAVARRFN-----LPGVRQGYGLTETTSAFIITPEGDDKPGASGKVVPLFKVKVIDLDTKKTLGVNRRGEICVKGPSLMLGYSNNPEATRETIDEEGWLHTGDIGYYDEDEHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPNIFDAGVAGVPDPDAGELPGAVVVMEKGKTMTEKEIVDYVNSQVVNHKRLRGGVRFVDEVPKGLTGKI 532          

HSP 2 Score: 56.6102 bits (135), Expect = 5.477e-7
Identity = 90/380 (23.68%), Postives = 153/380 (40.26%), Query Frame = 0

Query:   82 AKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLM--------VKILVVRDCPEEPDNH--VYSFCQQLAKV--DDAQFVS-DHLDIDQRMAIIW-SSGTTGMLKGIQHSFRIIANIINRGYGPL-----SAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYIC----PTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST----TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGXRDPDL------KPELMRYQIQESG 428
            A+ +++ G + ++ + L   N  E+F PVL  L     V+       P    Y ++E      +  P  +   RK    ++        +K +V+ D       H  + +F ++  ++    + FV  D  +  Q +A++  SSG+TG+ KG++ +        +    P+     S  T + +   FH G FG   ++   G  AC +    L   ++      +L L     Y+   ++L       +   S++  + DL ++  I     P A  + E    R +     L  V   Y LTE  +    +      P   G V P   VK++D DT++TLG N+ GEI  KG   P L       PE  R  I E G
Sbjct:   65 AEAMKNFGMKPEEHIALCSENCEEFFIPVLAGLYIGVAVA-------PTNEIYTLRELNHSLGIAQPTIVFSSRKGLPKVLEVQKTVTCIKKIVILDSKVNFGGHDCMETFIKKHVELGFQPSSFVPIDVKNRKQHVALLMNSSGSTGLPKGVRITHEGAVTRFSHAKDPIYGNQVSPGTAILTVVPFHHG-FGMFTTL---GYFACGYRVVMLTKFDE------ELFLRTLQDYKCTSVIL-VPTLFAILNKSELIDKFDLSNLTEIASGGAPLAKEVGEAVARRFN-----LPGVRQGYGLTETTSAFIITPEGDDKPGASGKVVPLFKVKVIDLDTKKTLGVNRRGEICVKG---PSLMLGYSNNPEATRETIDEEG 418          

BLAST of luciferin 4-monooxygenase-like vs. SwissProt
Match: gi|75290242|sp|Q6ETN3.1|4CL3_ORYSJ (RecName: Full=Probable 4-coumarate--CoA ligase 3; Short=4CL 3; Short=Os4CL3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 157.918 bits (398), Expect = 1.379e-39
Identity = 129/463 (27.86%), Postives = 217/463 (46.87%), Query Frame = 0

Query:  397 LGANQVGEIMAKGXRDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDI--DQRMAIIWSSGTTGMLKGIQHSFRINANIINR---GYGP----LSAQTILQSTCFFHAGGF-GFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALIT----FSTTPRHLGSVAPGTTV-----KIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKT 840
            LGA ++G   A    +P   P  +  Q + +GA+ +V     + +VR+   +  V ++ V        +  +  C +  +V  A+ +    D+  D  +A+ +SSGTTG+ KG+  + R     + +   G  P         IL     FH        ++ L  G T      + L             ++D   ++            +++ +  +V ++ DL S++ +   A+ + +D  +      P    +   Y +TE G ++     F+  P  + S + GT V     KIVD DT  +LG NQ GEI  +G +IM GYLN P+ T+  +D DG+LHTGD+G  D++  +F   RLKE+IK++G  + P E+E ++   P + + AV    D +  E+  A +VR EGS + E+ I   V + V  YKRI   V F  S+P+N  GKI+R+  + 
Sbjct:   95 LGAARLG--AATTTANPFYTPHEVHRQAEAAGARVIVTEACAVEKVREFAAERGVPVVTV--------DGAFDGCVEFREVLAAEELDADADVHPDDVVALPYSSGTTGLPKGVMLTHRSLITSVAQQVDGENPNLYFSKDDVILCLLPLFHIYSLNSVLLAGLRAGSTIVIMRKFDL-----------GALVDLVRKHNITIAPFVPPIVVEIAKSPRVTAE-DLASIRMVMSGAAPMGKDLQDAFMAKIPN-AVLGQGYGMTEAGPVLAMCLAFAKEPFKVKSGSCGTVVRNAELKIVDPDTGTSLGRNQSGEICIRGEQIMKGYLNDPEATKNTIDEDGWLHTGDIGFVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMKDDLAGEVPVAFIVRTEGSEITEDEIKKFVAKEVVFYKRIN-KVFFTDSIPKNPSGKILRKDLRA 533          

HSP 2 Score: 74.7146 bits (182), Expect = 1.316e-12
Identity = 85/357 (23.81%), Postives = 152/357 (42.58%), Query Frame = 0

Query:   72 SEMEPMSRKSAKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDI--DQRMAIIWSSGTTGMLKGIQHSFR-IIANIINR--GYGP----LSAQTILQSTCFFHAGGF-GFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGAL----ITFSTTPRHLGSVAPGTTV-----KIVDSDTQETLGANQVGEIMAKG 409
            +E+E  SR++A  LR MG    DV+  +L N  E+ F  LG        +  +P   P  +  Q + +GA+ +V     + +VR+   +  V ++ V        +  +  C +  +V  A+ +    D+  D  +A+ +SSGTTG+ KG+  + R +I ++  +  G  P         IL     FH        ++ L  G T      + L             ++D   ++            +++ +  +V ++ DL S++ +   A+ + +D  +      P    +   Y +TE G +    + F+  P  + S + GT V     KIVD DT  +LG NQ GEI  +G
Sbjct:   56 AEVESASRRAAAGLRRMGVGKGDVVMSLLRNCPEFAFSFLGAARLGAATTTANPFYTPHEVHRQAEAAGARVIVTEACAVEKVREFAAERGVPVVTV--------DGAFDGCVEFREVLAAEELDADADVHPDDVVALPYSSGTTGLPKGVMLTHRSLITSVAQQVDGENPNLYFSKDDVILCLLPLFHIYSLNSVLLAGLRAGSTIVIMRKFDL-----------GALVDLVRKHNITIAPFVPPIVVEIAKSPRVTAE-DLASIRMVMSGAAPMGKDLQDAFMAKIPN-AVLGQGYGMTEAGPVLAMCLAFAKEPFKVKSGSCGTVVRNAELKIVDPDTGTSLGRNQSGEICIRG 391          

BLAST of luciferin 4-monooxygenase-like vs. SwissProt
Match: gi|266484|sp|Q01158.1|LUCI_LUCLA (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 157.147 bits (396), Expect = 2.327e-39
Identity = 109/353 (30.88%), Postives = 175/353 (49.58%), Query Frame = 0

Query:  490 DQRMAIIWSSGTTGMLKGIQHSFRINANIINRGYGPL-----SAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST----TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKI 833
            +Q   I+ SSG+TG+ KG+Q +        +    P+     S  T + +   FH G FG   ++   G   C F    L   ++      +  L     Y+   ++L       +   S++  + DL ++  I    + + ++  E +   F  L  V   Y LTE  + I  +      P   G V P    K++D DT++TLG N+ GE+  KG  +M GY++ P+ T   +D +G+LHTGD+G+YDE    F   RLK LIK++G  + P E+E ++ + P + +  V G PDPI  EL  A+VV  +G ++ E  +++ V   V + KR+RGGV FV  +P+   GKI
Sbjct:  192 EQVALIMNSSGSTGLPKGVQLTHENAVTRFSHARDPIYGNQVSPGTAILTVVPFHHG-FGMFTTL---GYLTCGFRIVMLTKFDE------ETFLKTLQDYKCSSVIL-VPTLFAILNRSELLDKYDLSNLVEIASGGAPLSKEIGEAVARRF-NLPGVRQGYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKTLGPNRRGEVCVKGPMLMKGYVDNPEATREIIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPNIFDAGVAGVPDPIAGELPGAVVVLEKGKSMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKI 532          

BLAST of luciferin 4-monooxygenase-like vs. nr
Match: gi|383858595|ref|XP_003704786.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Megachile rotundata])

HSP 1 Score: 202.986 bits (515), Expect = 1.298e-52
Identity = 124/362 (34.25%), Postives = 187/362 (51.66%), Query Frame = 0

Query:  484 SDHLDIDQRMAIIWSSGTTGMLKGIQHSFRINANIINRGYG-PLSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALIT---FSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKTM 841
            SD  D +Q   I++SSGTTG+ KG+Q   R   NI+    G  +++  ++  T  +   G    I  + GG      P +  E            +     +Y+    ML +  A +L + S++  + DL S++ +C   S + ++    L   FP  K V   Y +TE G LI+     +TP   G     T +KI+D +T +TLG NQ GE+  K   +  GY   P+ T+  +D DG+LH+GDLG ++E G +F   RLKELIKF+G H+ P EIE +++  P V EVAV   P PI+ E   A V +     V E  +  LV  ++ DY ++RGGV F+PSLP  + GK+ R+  K +
Sbjct:  189 SDIDDTEQSAVILFSSGTTGLPKGVQLPHRALLNIMEVDEGLAVASHVLMWLTSLYWLTGTLLSIKHIVGGAKKVISPEFDEET-----------VCRHFEKYKVSWTMLSTSMANRLARFSRLH-EYDLSSLKILCTGGSAMGKEAEILLRKAFPTTK-VIQGYGMTELGNLISAQDVDSTPGSCGLATINTEIKIIDPETGKTLGPNQSGEVCIKNLSMTSGYYKNPEATKNAIDEDGWLHSGDLGCFNEKGELFIIDRLKELIKFQGYHVIPTEIESLLQSHPAVLEVAVVSIPHPIDCEHPVAFVSKIPNKEVTEEELKKLVANNLMDYCKLRGGVKFMPSLPHTASGKVARKELKAI 537          

HSP 2 Score: 84.7297 bits (208), Expect = 2.753e-13
Identity = 71/252 (28.17%), Postives = 111/252 (44.05%), Query Frame = 0

Query:  196 SDHLDIDQRMAIIWSSGTTGMLKGIQHSFRIIANIINRGYG-PLSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALIT---FSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGXRDPD---LKPELMRYQIQESGAKFVVCHPGNLA 440
            SD  D +Q   I++SSGTTG+ KG+Q   R + NI+    G  +++  ++  T  +   G    I  + GG      P +  E            +     +Y+    ML +  A +L + S++  + DL S++ +C   S + ++    L   FP  K V   Y +TE G LI+     +TP   G     T +KI+D +T +TLG NQ GE+  K           PE  +  I E G      H G+L 
Sbjct:  189 SDIDDTEQSAVILFSSGTTGLPKGVQLPHRALLNIMEVDEGLAVASHVLMWLTSLYWLTGTLLSIKHIVGGAKKVISPEFDEET-----------VCRHFEKYKVSWTMLSTSMANRLARFSRLH-EYDLSSLKILCTGGSAMGKEAEILLRKAFPTTK-VIQGYGMTELGNLISAQDVDSTPGSCGLATINTEIKIIDPETGKTLGPNQSGEVCIKNLSMTSGYYKNPEATKNAIDEDGW----LHSGDLG 423          

BLAST of luciferin 4-monooxygenase-like vs. nr
Match: gi|1059227323|ref|XP_017759168.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Eufriesea mexicana] >gi|1059227325|ref|XP_017759169.1| PREDICTED: 4-coumarate--CoA ligase 1-like [Eufriesea mexicana])

HSP 1 Score: 199.134 bits (505), Expect = 1.886e-51
Identity = 125/370 (33.78%), Postives = 189/370 (51.08%), Query Frame = 0

Query:  488 DIDQRMA-IIWSSGTTGMLKGIQHSFRINANIINRGYGP-----LSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYR--------PEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERL--HGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKTM 841
            DI +R A I+ SSGTTG  KG+  S R     I+   GP           L     FH   FG     +S G  AC    + LE           ++L +  R+R        P  +ML  H  ++         + D  SV+ I   A+ +P++    +       +++N + +  LT    +   +     +G + PG   K+VD+ T +TLGA Q GEI   G ++M+GY   PK T   +D + +LHTGDLG++ E+G ++  GR+KELIK++G  + P EIE II   PGV +VAV GKPD ++ E+  A+VVR  G NV+   I++   +++   K +RGGVIF+ SLP+   GKI+R+   +M
Sbjct:  183 DISKRAAAILCSSGTTGFPKGVMLSHRSLLIFIHTTKGPKLMDMRQGDRTLIFLPLFHGYAFGLINQTISSGGIACIMRNFNLE-----------MLLKSVERFRISHIPLVPPILVMLAKHPMVE---------KYDFSSVREITSGAAPLPQNVANEVKRRTKVGSIRNGYGMTELTIVSNISDKTCLDDTIGPIMPGFKCKVVDTKTGKTLGAGQSGEICFMGDQVMLGYYKNPKTTAETIDRENWLHTGDLGYFKEDGCLYITGRIKELIKYKGFQVSPSEIEAIILTHPGVKDVAVLGKPDELSGEVPMALVVRQPGKNVSVKEIVDFANENLSPQKWLRGGVIFMESLPKTPSGKILRKQLLSM 532          

HSP 2 Score: 73.9442 bits (180), Expect = 7.378e-10
Identity = 98/418 (23.44%), Postives = 164/418 (39.23%), Query Frame = 0

Query:   15 EDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCDLGLRGLTEDEVGLDCRMGSEMEPMSRKSAKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRY--QIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAK----VDDAQFVSDHLD-IDQRMA-IIWSSGTTGMLKGIQHSFRIIANIINRGYGP-----LSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYR--------PEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERL--HGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKG 409
            +DN I  D V+E    +L Q +   + TN D   L   D+      E +    CR   E+   S   + +L++ G   +D +     N   Y   +  TL      +  +P       R+  +I +    FV      +     +T +   K++ + D  +  + ++ +  + L K     D   F    +D I +R A I+ SSGTTG  KG+  S R +   I+   GP           L     FH   FG     +S G  AC    + LE           ++L +  R+R        P  +ML  H  ++         + D  SV+ I   A+ +P++    +       +++N + +  LT    +   +     +G + PG   K+VD+ T +TLGA Q GEI   G
Sbjct:    4 QDNIIYGDPVEEVPNVSLGQHLFDKLRTNADH--LAQIDI------ESDKHYTCR---EILEKSVILSTVLKNYGINIEDRISTASENHPNYILSICSTLFVGATFAPLNPAYTEREFRHMLEIYQPRVIFVSQRTEKIIAKVVSTVNWSTKLIQLED--QSLNRNIMTLKEILEKNRNIPDPYTFTPVQIDDISKRAAAILCSSGTTGFPKGVMLSHRSLLIFIHTTKGPKLMDMRQGDRTLIFLPLFHGYAFGLINQTISSGGIACIMRNFNLE-----------MLLKSVERFRISHIPLVPPILVMLAKHPMVE---------KYDFSSVREITSGAAPLPQNVANEVKRRTKVGSIRNGYGMTELTIVSNISDKTCLDDTIGPIMPGFKCKVVDTKTGKTLGAGQSGEICFMG 388          

BLAST of luciferin 4-monooxygenase-like vs. nr
Match: gi|1059876053|ref|XP_017793167.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Habropoda laboriosa] >gi|915659483|gb|KOC62438.1| Luciferin 4-monooxygenase [Habropoda laboriosa])

HSP 1 Score: 191.815 bits (486), Expect = 8.023e-49
Identity = 128/408 (31.37%), Postives = 207/408 (50.74%), Query Frame = 0

Query:  445 ATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQ----FVSDHLD--IDQRMAIIWSSGTTGMLKGIQHSFRINANIINRGYGPLSAQTILQSTCF---FHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSV-QYIC---PTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFS---TTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRR 836
            +T +   K++ + D  +  D ++ +  + L K +D      FV+  +D    +  AI+ SSGTTG+ KG+  S R     I      +  Q   ++  F   FH   FG    V+  G   C   ++ +E             L  T +YR   + L     + L +   V +  D  SV Q +C   P   N+ ++   R+      +K++ N Y +TE   +   S   T+   +G + PG   K+VD+ + + L   ++GEI   G ++M+GY   PK T   +D + +LHTGDLG+++E G+++  GR+KELIK++G  + P EIE +I K P V + AV G PD ++ EL  A+VVR  GSN++   I++    ++   KR+RGGV FV S+P+   GKI+RR
Sbjct:  136 STLNWTTKLISLDD--KTSDKNIVTLKELLEKSNDTANSCTFVATPIDDSAKKTSAILCSSGTTGLPKGVMLSHRNLLLFIKNMRIVIDIQQGDRAILFLPMFHGYAFGLINLVICAGAAICTMRSFEIEK-----------FLRLTEKYRITHLPLVPPILVTLAKHPTV-TNYDYGSVRQVLCGAAPLPKNVADEVKRRMK-----VKHIRNGYGMTELSIITNISDGTTSDDTVGPIVPGFKCKVVDTTSGKILAPGEIGEICFAGEQLMLGYYKNPKTTAETIDKENWLHTGDLGYFNEEGLLYITGRIKELIKYKGYQVSPSEIESVILKHPSVKDAAVMGIPDELSGELPMALVVRQPGSNISSKEIVDFTNSYLSPQKRLRGGVKFVASIPKTPSGKIVRR 524          

BLAST of luciferin 4-monooxygenase-like vs. nr
Match: gi|1325285932|ref|XP_023327697.1| (4-coumarate--CoA ligase-like [Eurytemora affinis])

HSP 1 Score: 191.045 bits (484), Expect = 1.610e-48
Identity = 149/471 (31.63%), Postives = 224/471 (47.56%), Query Frame = 0

Query:  397 LGANQVGEIMAKGXRDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLMV-----KILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDI-----DQRMAIIWSSGTTGMLKGIQHS-----FRINANIINRGYGPLSAQTI-LQSTCFFHAGGFGFPISVLSGGQTACFF-----PTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQE----LVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKTMY 842
             GA   G +++ G  DP L    ++ QIQ++ AK +VC   + ++  +A + L+       ILV+   P +P     S  + L + D      D   +      + + I W+SGTTG  KG+  S     F++  +        L  +TI L S  FFHAG F   +  L GG    F      P +T   +          I     +Y+P   M G++  +QL   S   ++ DL SV  I P  + I +    R+   FP LK V+  Y  TE G  ++ S T  ++G++  G    I D  T E LG  +VGEIMAK   +M+ YLNR  E+   LD +GF H GDLG+YDENGI+ +  R+KE+IK     I P E E+ +  +PGV E  V+     +N+E     +   V     S +N   IL L  + V+  +RI G +  + ++P N  GK +RR  + M+
Sbjct:   96 FGAWLCGGVVSVG--DPGLSASNLKSQIQDTHAKIIVCEVESCSKFLEANQSLLSAEQASNILVLDLEPWQPIPEGTSSFRSLYE-DQGDQCPDRASLPGYIPKEPVVIHWTSGTTGSPKGVVMSQEYLHFQMRQS-------KLPEKTIGLASNTFFHAGSF---LLSLDGGIRNKFSIHFIQPKHTTVES----------IFRFIEKYKPMFFMCGANDFVQLA--SSDLAKFDLSSVNVIMPAGNKIAKSAATRMKEKFPNLKFVYQFYGSTEVGG-VSGSFTVENMGALREGVQTYIRDQKTGEKLGVGEVGEIMAKTPTMMIHYLNRQDESRELLDEEGFAHMGDLGYYDENGILHYKERIKEMIKIGNYWIGPAEFEDNLENIPGVVEAVVW---QSMNKETDNYYIHGAVTLQASSGLNPEKILELANKDVEVKRRIDGTIHIINNMPHNPQGKKLRRTVRQMF 537          

HSP 2 Score: 152.91 bits (385), Expect = 1.847e-35
Identity = 130/424 (30.66%), Postives = 196/424 (46.23%), Query Frame = 0

Query:    1 MSLIKDKVLYSKMPEDNSIIPDIVKESLVEALFQKVQQNIETNGDFKFLINCDLGLRGLTEDEVGLDCRMGSEMEPMSRKSAKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLM-----VKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDHLDI-----DQRMAIIWSSGTTGMLKGIQHSFRIIANIINRGYGPLSAQTI-LQSTCFFHAGGFGFPISVLSGGQTACFF-----PTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAK 408
            MS I+DKVLYSK P       D  +E ++  L   +Q  +   GD  ++++C       TE+ V +   +G      S K A  L  +GF+  D LH       ++++P+ G  LC GVVS+GDP L    ++ QIQ++ AK +VC   + ++  +A + L+       ILV+   P +P     S  + L + D      D   +      + + I W+SGTTG  KG+  S   +   + +    L  +TI L S  FFHAG F   +  L GG    F      P +T   +          I     +Y+P   M G++  +QL   S   ++ DL SV  I P  + I +    R+   FP LK V+  Y  TE G  ++ S T  ++G++  G    I D  T E LG  +VGEIMAK
Sbjct:    1 MSEIRDKVLYSKEP----YTLDPGQERIIFKLLDLIQAELRETGDRDWILDC------TTENSVRISHILGR-----SIKVASGLTRLGFKPGDTLHTAYSTCLDFYWPIFGAWLCGGVVSVGDPGLSASNLKSQIQDTHAKIIVCEVESCSKFLEANQSLLSAEQASNILVLDLEPWQPIPEGTSSFRSLYE-DQGDQCPDRASLPGYIPKEPVVIHWTSGTTGSPKGVVMSQEYLHFQMRQ--SKLPEKTIGLASNTFFHAGSF---LLSLDGGIRNKFSIHFIQPKHTTVES----------IFRFIEKYKPMFFMCGANDFVQLA--SSDLAKFDLSSVNVIMPAGNKIAKSAATRMKEKFPNLKFVYQFYGSTEVGG-VSGSFTVENMGALREGVQTYIRDQKTGEKLGVGEVGEIMAK 390          

BLAST of luciferin 4-monooxygenase-like vs. nr
Match: gi|987915597|ref|XP_015433752.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Dufourea novaeangliae])

HSP 1 Score: 188.734 bits (478), Expect = 1.027e-47
Identity = 138/421 (32.78%), Postives = 206/421 (48.93%), Query Frame = 0

Query:  440 AQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDA----QFVSDHLDIDQRMA--IIWSSGTTGMLKGIQHSFR-----INANIINRGYGPLSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATH-RYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRH-----LGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKTMYS 843
            A + K    L  K++++    E  DN++ +  + L   +DA     FV   +    ++A  I+ SSGTTG  KG+  S R      NA  I           I+     FH   FG  +  +    T     T+ LE   Q +          TH    P  ++L + H I         S+ DL SV+ +   A+ +P D    +   F  +K++ N Y +TE   +  FS    +     +G   PG   KIV+ DT ETLG  Q GE+  K  + M+GY   PK T   +D D +LHTGDLG+Y+++G+++  GR KELIK++G  + P EIE +I+  P V + AV GKPD  + EL  A+VVR  GS ++   I+    Q++  +K +RGGV FV ++P+   GKI+RR    M S
Sbjct:  130 AIIAKIVSTLSWKVILIHLDNEPYDNNIPTIKKLLEVHNDAVNPYTFVPTPIGDSSKVAAVILCSSGTTGFPKGVMLSHRNLLLFTNAFSIPDMMDCRQGDRIINYLPQFHGYSFGLMLIGMIFTGTIYMMRTFNLETLLQSI-----QTYKITHLPMVPPILVLIAKHPIV--------SKYDLSSVKEVLCGAAPLPIDIANEIMRHF-KVKSIRNGYGMTELSIVTNFSIRNSNKNDGNVGPPVPGILSKIVNPDTGETLGPGQEGEVCFKTEQTMLGYFRNPKTTAETIDKDQWLHTGDLGYYNKDGLLYISGRFKELIKYKGYQVSPSEIEMMIQSHPDVKDAAVIGKPDEASGELPMALVVRQPGSKISPEDIMAFAKQNLSPHKWLRGGVKFVDAIPKTPSGKILRRELHNMIS 536          

HSP 2 Score: 64.3142 bits (155), Expect = 7.674e-7
Identity = 85/348 (24.43%), Postives = 137/348 (39.37%), Query Frame = 0

Query:   78 SRKSAKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDA----QFVSDHLDIDQRMA--IIWSSGTTGMLKGIQHSFRIIANIINRGYGP-----LSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATH-RYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRH-----LGSVAPGTTVKIVDSDTQETLGANQVGEIMAK 408
            S K A  L++ G   QD + +   N   Y   +        V +  +P       ++ ++    + +       A + K    L  K++++    E  DN++ +  + L   +DA     FV   +    ++A  I+ SSGTTG  KG+  S R +    N    P          I+     FH   FG  +  +    T     T+ LE   Q +          TH    P  ++L + H I         S+ DL SV+ +   A+ +P D    +   F  +K++ N Y +TE   +  FS    +     +G   PG   KIV+ DT ETLG  Q GE+  K
Sbjct:   56 SVKLAHALKTYGINAQDRVVITSENHPNYMVVMCAVFNAGAVFNPLNPTYTEREYKHMLEICEPRVIFVSRKTEAIIAKIVSTLSWKVILIHLDNEPYDNNIPTIKKLLEVHNDAVNPYTFVPTPIGDSSKVAAVILCSSGTTGFPKGVMLSHRNLLLFTNAFSIPDMMDCRQGDRIINYLPQFHGYSFGLMLIGMIFTGTIYMMRTFNLETLLQSI-----QTYKITHLPMVPPILVLIAKHPIV--------SKYDLSSVKEVLCGAAPLPIDIANEIMRHF-KVKSIRNGYGMTELSIVTNFSIRNSNKNDGNVGPPVPGILSKIVNPDTGETLGPGQEGEVCFK 389          

BLAST of luciferin 4-monooxygenase-like vs. nr
Match: gi|768409299|ref|XP_011552081.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Plutella xylostella])

HSP 1 Score: 188.348 bits (477), Expect = 1.126e-47
Identity = 122/351 (34.76%), Postives = 178/351 (50.71%), Query Frame = 0

Query:  493 MAIIWSSGTTGMLKGIQ--HSFRINANIINRGYGPLSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTT-----PRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRR 836
            + I++SSGTTG+ KG+Q  H   +    I +    L   T+L    +FH  G    +  L  G T  +   +           N +  L+   RY+P G        + L   S++    D+ SV+ +   A+++  +T   +   FP L  VF  Y +TE    +   T      P  +G++  G  VK+VD +T+E LG N+ GEI  K   +M GY+ +   TEA  D +GF  TGD+G+YDE+G  F   RLKELIK++G  + P +IE  + K P V + AV G+PD    E+  A VVR  GS V+   I   V   V + KR+RGGV FV  +P+N  GKI+RR
Sbjct:  183 LMILYSSGTTGLPKGVQITHYNGVVCCSIQKAPEGLDPMTVLSVAPWFHTMGLWGTVGNLCNGFTVVYMGKF-----------NEQRYLETIQRYKP-GYCAVVPPILVLLAKSQLLQDYDVSSVRMMYTGAASVDLETVREVKRRFPNLMEVFQGYGMTESTLAVARDTVDGGVKPGSVGTMPAGFVVKVVDLETREALGPNKPGEICVKSPTVMKGYIGK-NRTEA-FDDEGFYRTGDIGYYDEDGFFFIVDRLKELIKYKGYQVPPAQIEAELLKHPAVRDAAVVGRPDKAAGEVPVAFVVRQPGSEVSGEEIQEYVASKVSNPKRLRGGVRFVDEIPKNPSGKILRR 519          

HSP 2 Score: 83.9593 bits (206), Expect = 4.670e-13
Identity = 81/334 (24.25%), Postives = 144/334 (43.11%), Query Frame = 0

Query:   85 LRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDL-MVKILVVRDCPEEPDNHVYSFCQQLA-KVDDAQFVS-DHLDIDQRMAIIWSSGTTGMLKGIQ--HSFRIIANIINRGYGPLSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTT-----PRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAK 408
            L  MG +  D + +   N  +++  +LG +L    VS  +P    + +++ +     K + C P        + +   +++I V     +E   + Y F   ++ KV    F+  + +     + I++SSGTTG+ KG+Q  H   ++   I +    L   T+L    +FH  G    +  L  G T  +   +           N +  L+   RY+P G        + L   S++    D+ SV+ +   A+++  +T   +   FP L  VF  Y +TE    +   T      P  +G++  G  VK+VD +T+E LG N+ GEI  K
Sbjct:   64 LARMGVKSGDSVAICTENRLDFWPTLLGAMLSGASVSPINPMYTRDEIKHTLGICKPKIIFCSPSAHKAHETSIKSFNLIRIAVY----QEKIPNTYFFKDLISNKVRREDFLPVEVVGQTATLMILYSSGTTGLPKGVQITHYNGVVCCSIQKAPEGLDPMTVLSVAPWFHTMGLWGTVGNLCNGFTVVYMGKF-----------NEQRYLETIQRYKP-GYCAVVPPILVLLAKSQLLQDYDVSSVRMMYTGAASVDLETVREVKRRFPNLMEVFQGYGMTESTLAVARDTVDGGVKPGSVGTMPAGFVVKVVDLETREALGPNKPGEICVK 381          

BLAST of luciferin 4-monooxygenase-like vs. nr
Match: gi|321476815|gb|EFX87775.1| (hypothetical protein DAPPUDRAFT_311964 [Daphnia pulex])

HSP 1 Score: 188.348 bits (477), Expect = 1.324e-47
Identity = 131/371 (35.31%), Postives = 187/371 (50.40%), Query Frame = 0

Query:  487 LDIDQRMAIIWSSGTTGMLKGIQHSFRINANIINRGYGPLSAQTILQS-----TCFF----HAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRH-----LGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKTMYS 843
            LD+D    I++SSGTTG+ KG+  +   N N +      L +  I        T FF    HA            G      P +T E           L+L +   ++   M L    A+QL +   V+   DL S++ +   A+ I ++T   L   F  L  V  LY LTE   L T   T  +     +G VAP    KIVD++T E LG NQ GEI  +G  +M GY+     T+  +D+DG+LH+GD+G+YD++G  F   R KELIKF+G  + P E+E+I+   P + + AV   PD    EL  A +++  GS V EN I   +   V  +KR+RGGV+FV S+P+ + GKIMRR  KT+ S
Sbjct:  183 LDLDA--FILFSSGTTGVPKGVVLT---NLNYVVTRKQSLESTKISYRDPEDVTLFFMPLYHAATLNGLFECFMRGLRFVLMPNFTFE-----------LMLQSIQEFKITLMFLVPAIAVQLLK-HPVEKHYDLTSLKMLRSGAAAISKETLVALAEKFGVL--VLQLYGLTE-ATLCTHGNTFTYNRDGSIGVVAPFCESKIVDTETGEALGPNQEGEICVRGPIVMKGYIGNEAATKETVDTDGWLHSGDVGYYDDDGFFFITDRKKELIKFKGLQVSPTELEKILLGHPDIQDAAVAPVPDEAAGELPRAYIIKRPGSTVTENDIAKFIADQVSAHKRLRGGVVFVDSIPKTATGKIMRRQLKTIKS 533          

HSP 2 Score: 63.929 bits (154), Expect = 9.234e-7
Identity = 85/333 (25.53%), Postives = 138/333 (41.44%), Query Frame = 0

Query:   94 DVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQLAKVDDAQFVSDH-LDIDQRMAIIWSSGTTGMLKGIQHSFRIIAN---IINRGYGPLSAQTILQS----TCFF----HAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRH-----LGSVAPGTTVKIVDSDTQETLGANQVGEIMAKG 409
            +++ + LP   EY   VL   LC    +L +P      + + +Q +  K  V      ++  +   D+  +  ++    +  +   Y+   +  +    Q V +  LD+D    I++SSGTTG+ KG+     ++ N   ++ R     S +   +     T FF    HA            G      P +T E           L+L +   ++   M L    A+QL +   V+   DL S++ +   A+ I ++T   L   F  L  V  LY LTE   L T   T  +     +G VAP    KIVD++T E LG NQ GEI  +G
Sbjct:   77 EIVAVALPTCLEYSITVLALNLCGATSTLINPGQTISELTHCVQLTKPKLWVGTDSFSSKFSQLYPDVASRPPMILFGSKTSNELDYAGLIESGRGKTVQSVGNTPLDLDA--FILFSSGTTGVPKGV-----VLTNLNYVVTRKQSLESTKISYRDPEDVTLFFMPLYHAATLNGLFECFMRGLRFVLMPNFTFE-----------LMLQSIQEFKITLMFLVPAIAVQLLK-HPVEKHYDLTSLKMLRSGAAAISKETLVALAEKFGVL--VLQLYGLTE-ATLCTHGNTFTYNRDGSIGVVAPFCESKIVDTETGEALGPNQEGEICVRG 387          

BLAST of luciferin 4-monooxygenase-like vs. nr
Match: gi|768429780|ref|XP_011556085.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Plutella xylostella])

HSP 1 Score: 187.963 bits (476), Expect = 1.505e-47
Identity = 122/351 (34.76%), Postives = 178/351 (50.71%), Query Frame = 0

Query:  493 MAIIWSSGTTGMLKGIQ--HSFRINANIINRGYGPLSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTT-----PRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRR 836
            + I++SSGTTG+ KG+Q  H   +    I +    L   T+L    +FH  G    +  L  G T  +   +           N +  L+   RY+P G        + L   S++    D+ SV+ +   A+++  +T   +   FP L  VF  Y +TE    +   T      P  +G++  G  VK+VD +T E LG N+ GEI  K   +M GY+ +   TEA  D++GF  TGD+G+YDE+G  F   RLKELIK++G  + P +IE  + K P V + AV G+PD    E+  A VVR  GS V+   I   V   V + KR+RGGV FV  +P+N  GKI+RR
Sbjct:  183 LMILYSSGTTGLPKGVQITHYNGVVCCSIQKAPEGLDPMTVLSVAPWFHTMGLWGTVGNLCNGFTVVYMGKF-----------NERRYLETIQRYKP-GYCAVVPPILVLLAKSQLLQDYDVSSVRMMYTGAASVDLETVREVKRRFPNLMEVFQGYGMTESTLAVCRDTVDGGVKPGSVGTMPAGFVVKVVDLETHEALGPNKPGEICVKSPTVMKGYIGK-NRTEA-FDNEGFYRTGDIGYYDEDGFFFIVDRLKELIKYKGYQVPPAQIEAELLKHPAVRDAAVVGRPDKAAGEVPVAFVVRQPGSEVSGEEIQEYVASKVSNPKRLRGGVRFVDEIPKNPSGKILRR 519          

HSP 2 Score: 83.9593 bits (206), Expect = 4.316e-13
Identity = 81/334 (24.25%), Postives = 143/334 (42.81%), Query Frame = 0

Query:   85 LRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDL-MVKILVVRDCPEEPDNHVYSFCQQLA-KVDDAQFVS-DHLDIDQRMAIIWSSGTTGMLKGIQ--HSFRIIANIINRGYGPLSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTT-----PRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAK 408
            L  MG +  D + +   N  +++  +LG +L    VS  +P    + +++ +     K + C P        + +   +++I V     +E   + Y F   ++ KV    F+  + +     + I++SSGTTG+ KG+Q  H   ++   I +    L   T+L    +FH  G    +  L  G T  +   +           N +  L+   RY+P G        + L   S++    D+ SV+ +   A+++  +T   +   FP L  VF  Y +TE    +   T      P  +G++  G  VK+VD +T E LG N+ GEI  K
Sbjct:   64 LARMGVKSGDSVAICTENRLDFWPTLLGAMLSGASVSPINPMYSRDEIKHTLGICKPKIIFCSPAAHKAHETSIKSFNLIRIAVY----QEKIPNTYFFKDLISNKVRREDFLPVEVVGQTATLMILYSSGTTGLPKGVQITHYNGVVCCSIQKAPEGLDPMTVLSVAPWFHTMGLWGTVGNLCNGFTVVYMGKF-----------NERRYLETIQRYKP-GYCAVVPPILVLLAKSQLLQDYDVSSVRMMYTGAASVDLETVREVKRRFPNLMEVFQGYGMTESTLAVCRDTVDGGVKPGSVGTMPAGFVVKVVDLETHEALGPNKPGEICVK 381          

BLAST of luciferin 4-monooxygenase-like vs. nr
Match: gi|943963515|ref|XP_014364060.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Papilio machaon] >gi|930670165|gb|KPJ10988.1| Luciferin 4-monooxygenase [Papilio machaon])

HSP 1 Score: 187.578 bits (475), Expect = 2.212e-47
Identity = 122/390 (31.28%), Postives = 196/390 (50.26%), Query Frame = 0

Query:  472 QQLAKVDDAQFVSDHLDIDQRMA-----------IIWSSGTTGMLKG--IQHSFRINANIINRGYGPLSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST-------TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVVRAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRRHAKTM 841
            + LA V     +  ++++++ +A           I++SSGTTG+ KG  I H   I    +     P     +L  T ++HA G    +  +  G    F P + +            + L +  +Y+   ++L     + L   +K D + D+ SVQ I   A+ + +DT + +   FP L +V+  Y +TE   +IT ++        P  +G V   T VKIVD DT+E LG N+ GE+  K   +M GY+   K+  A  D +GF  TGD+ +YD+   ++   RLKEL+K++G  + P EIE ++ + PGV +V V G PD    EL  A VV   G+N+ E  I + V + + + K +RGGV FV  +P+N  GKI+R+  + M
Sbjct:  161 KDLAIVSTGSLIKKNVNVEEFLAVEVKGQTDTAFIMYSSGTTGLPKGVMISHVNVIATCAVKDVCDP--KLVVLNITPWYHAMGLMLMLVYMGHGTKTVFLPKFDIN-----------IYLQSIEKYKANQLILVPPVILAL---TKTDIKYDVSSVQVILSGAAVLQKDTIDAVKKKFPNLIDVYQGYGMTEATVMITLNSHVNGKQCKPGSVGKVVNNTVVKIVDIDTREPLGPNEKGELCFKSPMVMKGYVG--KDRSADFDDEGFYKTGDIAYYDDEKYIYIVDRLKELVKYKGFQVPPAEIETVLMQHPGVRDVGVVGLPDENAGELPLAFVVPQTGANITEKEIQDFVAERLSNPKHLRGGVRFVKEIPKNPSGKILRKVLRQM 532          

HSP 2 Score: 83.1889 bits (204), Expect = 8.706e-13
Identity = 66/245 (26.94%), Postives = 111/245 (45.31%), Query Frame = 0

Query:  184 QQLAKVDDAQFVSDHLDIDQRMA-----------IIWSSGTTGMLKG--IQHSFRIIANIINRGYGPLSAQTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLILDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYICPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFST-------TPRHLGSVAPGTTVKIVDSDTQETLGANQVGEIMAK 408
            + LA V     +  ++++++ +A           I++SSGTTG+ KG  I H   I    +     P     +L  T ++HA G    +  +  G    F P + +            + L +  +Y+   ++L     + L   +K D + D+ SVQ I   A+ + +DT + +   FP L +V+  Y +TE   +IT ++        P  +G V   T VKIVD DT+E LG N+ GE+  K
Sbjct:  161 KDLAIVSTGSLIKKNVNVEEFLAVEVKGQTDTAFIMYSSGTTGLPKGVMISHVNVIATCAVKDVCDP--KLVVLNITPWYHAMGLMLMLVYMGHGTKTVFLPKFDIN-----------IYLQSIEKYKANQLILVPPVILAL---TKTDIKYDVSSVQVILSGAAVLQKDTIDAVKKKFPNLIDVYQGYGMTEATVMITLNSHVNGKQCKPGSVGKVVNNTVVKIVDIDTREPLGPNEKGELCFK 389          

BLAST of luciferin 4-monooxygenase-like vs. nr
Match: gi|196009249|ref|XP_002114490.1| (hypothetical protein TRIADDRAFT_27462 [Trichoplax adhaerens] >gi|190583509|gb|EDV23580.1| hypothetical protein TRIADDRAFT_27462 [Trichoplax adhaerens])

HSP 1 Score: 187.193 bits (474), Expect = 4.213e-47
Identity = 144/444 (32.43%), Postives = 233/444 (52.48%), Query Frame = 0

Query:  418 ELMRYQIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQL-AKVDDA--QF-VSDHLDIDQRMAIIWSSGTTGMLKGIQHS-FRINANIIN---RGYGPLSA----QTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLI----LDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYI----CPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRH----LGSVAPGTTVKIVDSDTQETLGANQVGEIMAKGTRIMMGYLNRPKETEACLDSDGFLHTGDLGHYDENGIMFFDGRLKELIKFEGKHIYPMEIEEIIRKLPGVSEVAVFGKPDPINQELVTAMVV-RAEGSNVNENTILNLVTQHVDDYKRIRGGVIFVPSLPRNSIGKIMRR 836
            EL R Q+ +S   +++ H   +  V+K       KI++ +  P   DN   +    +  KV+ A  +F VS   D+     I++SSGTTG+ KG+  S + + A+++N   R  G L+     + +L     FH  G    +S+LS          +TL N  + LV  SK I    L+A   Y+   ++      + L +   +D+  +L S+ YI     P +S I ED  +RL      L+     Y +TE G L+ ++    +    +G + P T  K+++ D  E +G N+VGE+  +G ++M+GY+N P+ T   +D +G+LHTGD+G+YD  G +F   RLKELIKF+G  I P E+E I+     +++ AV G PD    E+  A VV R    +++   I+  V ++V  YK++RGGV FV S+P+++ GKI+RR
Sbjct:  108 ELAR-QLNDSKPAYIITHESCINTVKKVNYSFRRKIVIGK--PPIRDNSYQAMFDLINGKVNKAPRKFKVSPKSDV---AVILYSSGTTGLPKGVMLSHYNLIADMVNFSHRDIGALNMDSEDECLLNVLPLFHVYGL---VSILS----------FTLCNGRR-LVLQSKFIHTKFLNAIQEYKVTKLLCVPAMVLFLAKSPLIDN-YNLSSLTYISSGGAPVSSEIGEDLQKRLK-----LQTFNQGYGMTELGPLVVYAFLNSYKVGSVGKLVPNTDCKVINVDNGEAVGLNEVGELCFRGPQMMLGYINNPQATADTIDKNGWLHTGDIGYYDNEGNVFVIDRLKELIKFKGFQIAPAELEAILNDHQQIADSAVIGIPDETAGEVPKAYVVLRNSKDSLSAKDIIKYVAENVAWYKQLRGGVEFVQSIPKSASGKILRR 525          

HSP 2 Score: 91.2781 bits (225), Expect = 2.171e-15
Identity = 106/400 (26.50%), Postives = 180/400 (45.00%), Query Frame = 0

Query:   40 IETNGDFKFLI--NCD--LGLRGLTED--EVGLDCRMGSEMEPMSRKSAKLLRSMGFEFQDVLHLVLPNTTEYFFPVLGTLLCQGVVSLGDPDLKPELMRYQIQESGAKFVVCHPGNLAQVRKATRDLMVKILVVRDCPEEPDNHVYSFCQQL-AKVDDA--QF-VSDHLDIDQRMAIIWSSGTTGMLKGIQHS-FRIIANIIN---RGYGPLSA----QTILQSTCFFHAGGFGFPISVLSGGQTACFFPTYTLENTEQGLVGNSKLI----LDATHRYRPEGMMLGSHHAIQLCQISKVDSQLDLDSVQYI----CPTASNIPEDTYERLHGVFPALKNVFNLYSLTEFGALITFSTTPRH----LGSVAPGTTVKIVDSDTQETLGANQVGEIMAKG 409
            I  N DF   I  NCD       L +D  E       G   + + R  + L+R +G + +DV+ +  PN  E+       +   G+V+  +P      +  Q+ +S   +++ H   +  V+K       KI++ +  P   DN   +    +  KV+ A  +F VS   D+     I++SSGTTG+ KG+  S + +IA+++N   R  G L+     + +L     FH  G    +S+LS          +TL N  + LV  SK I    L+A   Y+   ++      + L +   +D+  +L S+ YI     P +S I ED  +RL      L+     Y +TE G L+ ++    +    +G + P T  K+++ D  E +G N+VGE+  +G
Sbjct:   12 IPDNIDFYQFIFRNCDQYYNRIALIDDDKEEAKSITYGELRDRIERCCSGLIR-LGLQPKDVVMIRSPNCLEFVIGFFAVIAAGGIVTTCNPIFTEHELARQLNDSKPAYIITHESCINTVKKVNYSFRRKIVIGK--PPIRDNSYQAMFDLINGKVNKAPRKFKVSPKSDV---AVILYSSGTTGLPKGVMLSHYNLIADMVNFSHRDIGALNMDSEDECLLNVLPLFHVYGL---VSILS----------FTLCNGRR-LVLQSKFIHTKFLNAIQEYKVTKLLCVPAMVLFLAKSPLIDN-YNLSSLTYISSGGAPVSSEIGEDLQKRLK-----LQTFNQGYGMTELGPLVVYAFLNSYKVGSVGKLVPNTDCKVINVDNGEAVGLNEVGELCFRG 385