BLAST of EMLSAG00000000339 vs. GO
Match: - (symbol:PIAS1 "cDNA FLJ37322 fis, clone BRAMY2018126, highly similar to Protein inhibitor of activated STAT protein 1" species:9606 "Homo sapiens" [GO:0003676 "nucleic acid binding" evidence=IEA] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0019789 "SUMO ligase activity" evidence=IEA] InterPro:IPR003034 InterPro:IPR004181 InterPro:IPR027227 Pfam:PF02891 PROSITE:PS50800 PROSITE:PS51044 SMART:SM00513 GO:GO:0008152 GO:GO:0008270 GO:GO:0003676 Gene3D:1.10.720.30 EMBL:AC107871 GO:GO:0019789 InterPro:IPR023321 Pfam:PF14324 PROSITE:PS51466 EMBL:AC135628 UniGene:Hs.162458 HGNC:HGNC:2752 HOVERGEN:HBG053598 PANTHER:PTHR10782:SF11 HOGENOM:HOG000230594 OMA:LRENHGH EMBL:AK094641 Ensembl:ENST00000545237 NextBio:35469807 Uniprot:B3KSY9)

HSP 1 Score: 317.775 bits (813), Expect = 2.524e-96
Identity = 158/325 (48.62%), Postives = 224/325 (68.92%), Query Frame = 0

Query:  225 PHTKTVVPIQNSEPKYDIANNGHL---MYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATG-KLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEALPSKDE 543
            P +  ++P+    PK+++    HL   ++P  PD++L  LPFY +   L+KP+SL   +S RF+   F F LTP Q   I+ S   D +G K ++  Q+Q+RFCL+ETSCPQ+D+FP ++CV VN +  +LP  +P  K GVEPKRP +PINITS  +LS T+PN + V+WT E G+ + +A+Y V++L+S  L++ L+ KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LYIQMNE+KP W+CPVC+K A YE+L++DG F E+L   K  T+ +EI  K D  W  + SK E
Sbjct:  105 PASSPLLPVSLLGPKHEL-ELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSS--MDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEIL---KYCTDCDEIQFKEDGTWAPMRSKKE 423          

HSP 2 Score: 58.5362 bits (140), Expect = 1.651e-7
Identity = 27/54 (50.00%), Postives = 41/54 (75.93%), Query Frame = 0

Query:    7 VQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL 59
            V+ MVM+ R+SELQ L+ YAG++K GRK +L  +AL+L++   S  ++MKI+EL
Sbjct:    9 VKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKEL 62          

BLAST of EMLSAG00000000339 vs. GO
Match: - (symbol:PIAS1 "E3 SUMO-protein ligase PIAS1" species:9606 "Homo sapiens" [GO:0000122 "negative regulation of transcription from RNA polymerase II promoter" evidence=IEA] [GO:0003677 "DNA binding" evidence=IEA] [GO:0003713 "transcription coactivator activity" evidence=NAS] [GO:0003714 "transcription corepressor activity" evidence=TAS] [GO:0005515 "protein binding" evidence=IPI] [GO:0005634 "nucleus" evidence=IDA] [GO:0005654 "nucleoplasm" evidence=TAS] [GO:0006351 "transcription, DNA-templated" evidence=IEA] [GO:0007259 "JAK-STAT cascade" evidence=TAS] [GO:0007283 "spermatogenesis" evidence=IEA] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0016607 "nuclear speck" evidence=IEA] [GO:0016925 "protein sumoylation" evidence=IEA;ISS] [GO:0019221 "cytokine-mediated signaling pathway" evidence=TAS] [GO:0019789 "SUMO ligase activity" evidence=IDA] [GO:0019899 "enzyme binding" evidence=IPI] [GO:0019904 "protein domain specific binding" evidence=IEA] [GO:0030521 "androgen receptor signaling pathway" evidence=NAS] [GO:0031625 "ubiquitin protein ligase binding" evidence=IEA] [GO:0032436 "positive regulation of proteasomal ubiquitin-dependent protein catabolic process" evidence=ISS] [GO:0033235 "positive regulation of protein sumoylation" evidence=IDA] [GO:0042127 "regulation of cell proliferation" evidence=ISS] [GO:0045893 "positive regulation of transcription, DNA-templated" evidence=NAS] [GO:0050681 "androgen receptor binding" evidence=NAS] [GO:0051152 "positive regulation of smooth muscle cell differentiation" evidence=IEA] [GO:0060333 "interferon-gamma-mediated signaling pathway" evidence=TAS] [GO:0060334 "regulation of interferon-gamma-mediated signaling pathway" evidence=TAS] [GO:0065004 "protein-DNA complex assembly" evidence=IEA] [GO:0016605 "PML body" evidence=ISS] InterPro:IPR003034 InterPro:IPR004181 InterPro:IPR027227 Pfam:PF02891 PROSITE:PS50800 PROSITE:PS51044 SMART:SM00513 UniPathway:UPA00886 EMBL:AF077951 Reactome:REACT_6900 GO:GO:0003714 GO:GO:0045893 GO:GO:0005654 GO:GO:0060333 GO:GO:0030521 GO:GO:0003677 GO:GO:0016607 GO:GO:0016605 GO:GO:0008270 GO:GO:0007283 GO:GO:0006351 GO:GO:0042127 GO:GO:0000122 GO:GO:0003713 Gene3D:1.10.720.30 GO:GO:0033235 GO:GO:0032436 GO:GO:0050681 GO:GO:0016925 EMBL:AC107871 GO:GO:0007259 GO:GO:0060334 GO:GO:0019789 GO:GO:0065004 eggNOG:NOG125513 InterPro:IPR023321 Pfam:PF14324 PROSITE:PS51466 EMBL:AF167160 EMBL:AK314515 EMBL:AC135628 EMBL:BC118587 EMBL:BC121797 EMBL:U78524 RefSeq:NP_057250.1 UniGene:Hs.162458 PDB:1V66 PDBsum:1V66 ProteinModelPortal:O75925 SMR:O75925 BioGrid:114124 DIP:DIP-5970N IntAct:O75925 MINT:MINT-132657 STRING:9606.ENSP00000249636 PhosphoSite:O75925 PaxDb:O75925 PRIDE:O75925 Ensembl:ENST00000249636 GeneID:8554 KEGG:hsa:8554 UCSC:uc002aqz.3 CTD:8554 GeneCards:GC15P068346 HGNC:HGNC:2752 HPA:CAB012304 MIM:603566 neXtProt:NX_O75925 PharmGKB:PA33285 HOVERGEN:HBG053598 InParanoid:O75925 KO:K04706 OrthoDB:EOG7HF1JB PhylomeDB:O75925 TreeFam:TF323787 SignaLink:O75925 EvolutionaryTrace:O75925 GeneWiki:PIAS1 GenomeRNAi:8554 NextBio:32053 PMAP-CutDB:O75925 PRO:PR:O75925 ArrayExpress:O75925 Bgee:O75925 CleanEx:HS_PIAS1 Genevestigator:O75925 GO:GO:0051152 PANTHER:PTHR10782:SF11 Uniprot:O75925)

HSP 1 Score: 317.39 bits (812), Expect = 2.534e-96
Identity = 158/325 (48.62%), Postives = 224/325 (68.92%), Query Frame = 0

Query:  225 PHTKTVVPIQNSEPKYDIANNGHL---MYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATG-KLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEALPSKDE 543
            P +  ++P+    PK+++    HL   ++P  PD++L  LPFY +   L+KP+SL   +S RF+   F F LTP Q   I+ S   D +G K ++  Q+Q+RFCL+ETSCPQ+D+FP ++CV VN +  +LP  +P  K GVEPKRP +PINITS  +LS T+PN + V+WT E G+ + +A+Y V++L+S  L++ L+ KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LYIQMNE+KP W+CPVC+K A YE+L++DG F E+L   K  T+ +EI  K D  W  + SK E
Sbjct:  103 PASSPLLPVSLLGPKHEL-ELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSS--MDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEIL---KYCTDCDEIQFKEDGTWAPMRSKKE 421          

HSP 2 Score: 60.8474 bits (146), Expect = 3.239e-8
Identity = 29/60 (48.33%), Postives = 44/60 (73.33%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL 59
            MAD   ++ MVM+ R+SELQ L+ YAG++K GRK +L  +AL+L++   S  ++MKI+EL
Sbjct:    1 MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKEL 60          

BLAST of EMLSAG00000000339 vs. GO
Match: - (symbol:Pias2 "protein inhibitor of activated STAT 2" species:10090 "Mus musculus" [GO:0003676 "nucleic acid binding" evidence=IEA] [GO:0003677 "DNA binding" evidence=IDA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005634 "nucleus" evidence=ISO;IDA] [GO:0006351 "transcription, DNA-templated" evidence=IDA] [GO:0006355 "regulation of transcription, DNA-templated" evidence=ISO] [GO:0006357 "regulation of transcription from RNA polymerase II promoter" evidence=IDA] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0016604 "nuclear body" evidence=IDA] [GO:0016605 "PML body" evidence=ISO] [GO:0016874 "ligase activity" evidence=IEA] [GO:0016925 "protein sumoylation" evidence=ISO;IDA] [GO:0019789 "SUMO ligase activity" evidence=ISO;IDA] [GO:0019904 "protein domain specific binding" evidence=ISO] [GO:0030331 "estrogen receptor binding" evidence=ISO] [GO:0030521 "androgen receptor signaling pathway" evidence=ISO] [GO:0031625 "ubiquitin protein ligase binding" evidence=IPI] [GO:0035259 "glucocorticoid receptor binding" evidence=ISO] [GO:0045667 "regulation of osteoblast differentiation" evidence=IMP] [GO:0045893 "positive regulation of transcription, DNA-templated" evidence=ISO;IDA] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IDA] [GO:0046872 "metal ion binding" evidence=IEA] [GO:0050681 "androgen receptor binding" evidence=ISO] [GO:0050775 "positive regulation of dendrite morphogenesis" evidence=ISO] [GO:0060765 "regulation of androgen receptor signaling pathway" evidence=ISO] [GO:0060766 "negative regulation of androgen receptor signaling pathway" evidence=ISO] InterPro:IPR003034 InterPro:IPR004181 InterPro:IPR027228 Pfam:PF02891 PROSITE:PS50800 PROSITE:PS51044 SMART:SM00513 UniPathway:UPA00886 MGI:MGI:1096566 GO:GO:0006355 GO:GO:0003677 GO:GO:0016607 GO:GO:0016605 GO:GO:0008270 GO:GO:0045944 GO:GO:0006351 Gene3D:1.10.720.30 GO:GO:0050681 GO:GO:0060765 GO:GO:0016604 GO:GO:0045667 GO:GO:0016925 GO:GO:0019789 GO:GO:0060766 eggNOG:NOG125513 GeneTree:ENSGT00550000074410 InterPro:IPR023321 Pfam:PF14324 PROSITE:PS51466 HOVERGEN:HBG053598 OrthoDB:EOG7HF1JB TreeFam:TF323787 CTD:9063 KO:K16063 OMA:ICISRDF PANTHER:PTHR10782:SF12 EMBL:AF201391 EMBL:AK029716 EMBL:AK014871 EMBL:AK078813 EMBL:AK086653 EMBL:BC005596 EMBL:BC034711 EMBL:AF039567 RefSeq:NP_001157639.1 RefSeq:NP_001157640.1 RefSeq:NP_001157641.1 RefSeq:NP_001157642.1 RefSeq:NP_032628.3 UniGene:Mm.6370 ProteinModelPortal:Q8C5D8 SMR:Q8C5D8 BioGrid:201429 IntAct:Q8C5D8 MINT:MINT-1782012 PhosphoSite:Q8C5D8 PaxDb:Q8C5D8 PRIDE:Q8C5D8 Ensembl:ENSMUST00000114777 GeneID:17344 KEGG:mmu:17344 UCSC:uc008frb.1 UCSC:uc008frc.2 UCSC:uc012bfb.1 InParanoid:Q8C5D8 NextBio:291922 PRO:PR:Q8C5D8 ArrayExpress:Q8C5D8 Bgee:Q8C5D8 CleanEx:MM_PIAS2 Genevestigator:Q8C5D8 Uniprot:Q8C5D8)

HSP 1 Score: 316.62 bits (810), Expect = 3.112e-96
Identity = 171/397 (43.07%), Postives = 244/397 (61.46%), Query Frame = 0

Query:  231 VPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEESSSSSQKPKSIQNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATTTNKASEDKIVECITLDDSDEESPANPPAKK 626
            V +Q+++P +++      + P  PDVQL +LPFY V  +L+KP+SL  S   RFQ + F+F LTP Q   I  S      G+ +Y  Q+Q+R CL ETSCPQ+DN+P S+C+ VNG+   LP   P  K G+E KRP +P+NITS  +LS+ +PN ++++W  E GK + +++Y V +LTS  L++ LK KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LY+QMNE+KP WICPVC+K A YE+L++DG F E+LND      +EI  + D  W  +  K E    +SQ                T VE+  V+          +K  S+  A+  +K   D +++      SDEE   +PPAK+
Sbjct:  121 VLLQDTKPTFEMQQPSPPIPPVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILND--CSDVDEIKFQEDGSWCPMRPKKEAMKVTSQP--------------CTKVESSSVF----------SKPCSVTVASDASKKKID-VIDLTIESSSDEEE--DPPAKR 488          

HSP 2 Score: 66.6254 bits (161), Expect = 4.064e-10
Identity = 32/60 (53.33%), Postives = 47/60 (78.33%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL 59
            MAD E ++ MV +FR+SELQ L+ +AG++KSGRK DL +RAL+L++   S  +++KIREL
Sbjct:    1 MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIREL 60          

BLAST of EMLSAG00000000339 vs. GO
Match: - (symbol:PIAS2 "Uncharacterized protein" species:9913 "Bos taurus" [GO:0003676 "nucleic acid binding" evidence=IEA] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0016605 "PML body" evidence=IEA] [GO:0019789 "SUMO ligase activity" evidence=IEA] [GO:0031625 "ubiquitin protein ligase binding" evidence=IEA] [GO:0045667 "regulation of osteoblast differentiation" evidence=IEA] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IEA] [GO:0060766 "negative regulation of androgen receptor signaling pathway" evidence=IEA] InterPro:IPR003034 InterPro:IPR004181 InterPro:IPR027228 Pfam:PF02891 PROSITE:PS50800 PROSITE:PS51044 SMART:SM00513 GO:GO:0008152 GO:GO:0016605 GO:GO:0008270 GO:GO:0045944 GO:GO:0003676 Gene3D:1.10.720.30 GO:GO:0045667 GO:GO:0019789 GO:GO:0060766 GeneTree:ENSGT00550000074410 InterPro:IPR023321 Pfam:PF14324 PROSITE:PS51466 OrthoDB:EOG7HF1JB TreeFam:TF323787 OMA:ICISRDF PANTHER:PTHR10782:SF12 EMBL:DAAA02056922 Ensembl:ENSBTAT00000010308 Uniprot:F1MMY9)

HSP 1 Score: 315.849 bits (808), Expect = 4.748e-96
Identity = 163/353 (46.18%), Postives = 230/353 (65.16%), Query Frame = 0

Query:  231 VPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEESSSSSQK-PKSIQNPIIAPPVNNT-----SVETVDVYD 576
            V +Q+++P +++      + P  PDVQL +LPFY V  +L+KP+SL  S   RFQ + F+F LTP Q   I  S      G+ +Y  Q+Q+R CL ETSCPQ+DN+P S+C+ VNG+   LP   P  K G+E KRP +P+NITS  +LS+ +PN ++++W  E GK + +++Y V +LTS  L++ LK KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LY+QMNE+KP WICPVC+K A YE+L++DG F E+LND      +EI  + D  W  +  K E    S+Q  PK   + +++ P   T     S + VDV D
Sbjct:  121 VLLQDTKPAFEMQQPSPPIPPVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILND--CTDVDEIKFQEDGSWCPMRPKKEAMKVSTQPCPKIETSTVLSKPCAVTVASEPSKKKVDVID 471          

HSP 2 Score: 61.2326 bits (147), Expect = 2.476e-8
Identity = 36/81 (44.44%), Postives = 54/81 (66.67%), Query Frame = 0

Query:   10 MVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL-NNEMPRPGIGIGNNNK-ESPLNQLLGERS 87
            MV +FR+SELQ L+ +AG++KSGRK DL +RAL+L++   S  +++KIREL     PR   G+ + +  +SP+  L G  S
Sbjct:   10 MVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLEGLSDLSAIKSPVFSLDGSSS 90          

BLAST of EMLSAG00000000339 vs. GO
Match: - (symbol:pias1b "protein inhibitor of activated STAT, 1b" species:7955 "Danio rerio" [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0019789 "SUMO ligase activity" evidence=IEA] [GO:0003676 "nucleic acid binding" evidence=IEA] InterPro:IPR003034 InterPro:IPR004181 InterPro:IPR027227 Pfam:PF02891 PROSITE:PS50800 PROSITE:PS51044 SMART:SM00513 ZFIN:ZDB-GENE-050419-202 GO:GO:0008152 GO:GO:0008270 GO:GO:0003676 Gene3D:1.10.720.30 GO:GO:0019789 eggNOG:NOG125513 GeneTree:ENSGT00550000074410 InterPro:IPR023321 Pfam:PF14324 PROSITE:PS51466 HOVERGEN:HBG053598 KO:K04706 OrthoDB:EOG7HF1JB TreeFam:TF323787 PANTHER:PTHR10782:SF11 HOGENOM:HOG000230594 OMA:LRENHGH EMBL:BX247951 EMBL:BX890624 RefSeq:XP_005159210.1 UniGene:Dr.79854 ProteinModelPortal:A8E7T0 SMR:A8E7T0 STRING:7955.ENSDARP00000084743 Ensembl:ENSDART00000090310 GeneID:562982 KEGG:dre:562982 CTD:562982 Uniprot:A8E7T0)

HSP 1 Score: 316.62 bits (810), Expect = 5.766e-96
Identity = 175/380 (46.05%), Postives = 245/380 (64.47%), Query Frame = 0

Query:  178 PTRLHSKKSANLPNILKKSPRGHISNTGSKTIISSLLPTIKTADILVPH-TKTVVPIQNSEPKYDIANNGHL---MYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATG-KLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKD--EESSSSS 549
            PT++ S    +LP I       H S+ G+    S+ LP   T      H + +++P+    PK+++    HL   ++P  PDV+L  LPFY V   L+KP+SL   +S RFQ   F F LTP Q   I+ S   D +G K ++  Q+Q+RFCL+ETSCPQ+D+FP ++CV VNG+   LP  +P  K GVEPKRP +PINITS  +LS T+PN + V+WT E G+ + +A+Y V + +S+ L++ L+ KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+ +PCRA TC HLQCFDA LYIQMNE+KP W+CPVC+K A YE+L++DG F E+L+       +EI  K D  W  + SK   +E S+SS
Sbjct:   57 PTKMVSPTDLSLPGI-------HSSSGGA----SAGLPASLTQLGYDGHGSPSLLPVALLGPKHELGLP-HLPSALHPVHPDVKLQRLPFYDVLDELIKPTSLASDNSQRFQETCFAFALTPQQVQQISSS--MDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNGKPCNLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTSEIGRSYSMAVYLVRQQSSSVLLQRLRSKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLMIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILSS--CVDCDEIQFKEDGNWSPMRSKKIVQEVSASS 420          

HSP 2 Score: 56.225 bits (134), Expect = 7.173e-7
Identity = 26/51 (50.98%), Postives = 39/51 (76.47%), Query Frame = 0

Query:   10 MVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL 59
            MVM+ R+SELQ L+ YAG++K GRK +L  +AL+L++   S  ++MKI+EL
Sbjct:    1 MVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKTGCSPAVQMKIKEL 51          

BLAST of EMLSAG00000000339 vs. GO
Match: - (symbol:pias2 "protein inhibitor of activated STAT, 2" species:7955 "Danio rerio" [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0019789 "SUMO ligase activity" evidence=IEA] [GO:0003676 "nucleic acid binding" evidence=IEA] InterPro:IPR003034 InterPro:IPR004181 InterPro:IPR027228 Pfam:PF02891 PROSITE:PS50800 PROSITE:PS51044 SMART:SM00513 ZFIN:ZDB-GENE-060424-1 GO:GO:0008152 GO:GO:0008270 EMBL:CR318632 GO:GO:0003676 Gene3D:1.10.720.30 GO:GO:0019789 eggNOG:NOG125513 GeneTree:ENSGT00550000074410 InterPro:IPR023321 Pfam:PF14324 PROSITE:PS51466 HOVERGEN:HBG053598 OrthoDB:EOG7HF1JB TreeFam:TF323787 CTD:9063 KO:K16063 OMA:ICISRDF PANTHER:PTHR10782:SF12 RefSeq:XP_685704.3 UniGene:Dr.25643 ProteinModelPortal:A2CE48 SMR:A2CE48 PRIDE:A2CE48 Ensembl:ENSDART00000142538 GeneID:564149 KEGG:dre:564149 InParanoid:A2CE48 NextBio:20885246 PRO:PR:A2CE48 ArrayExpress:A2CE48 Uniprot:A2CE48)

HSP 1 Score: 313.923 bits (803), Expect = 2.852e-95
Identity = 156/319 (48.90%), Postives = 208/319 (65.20%), Query Frame = 0

Query:  233 IQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQ-VQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEESSSSS 549
               S+P  ++     LM P  PDVQ+ SLPFY V  +L+KPSSL      RF   + F+F LTP Q   +  S      G+ +Y  QIQ+RFCL ETSCPQ+DN+P  +C+ VNG+   LP   P  K GVE KRP +P+NITS  +LS+ +PN + VTW  E GK + +++Y V +LTS  L++ L+ KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LY+QMNE+KP WICPVC+K A YE+L++DG F E+LND      +EI  + D  W  +  K E    SS
Sbjct:  121 FHESKPMMNMQQPTTLMAPVHPDVQMKSLPFYDVLDVLIKPSSLGAHAVQRFHNEKYFIFALTPQQVREVCISRDFLPGGRRDYMVQIQLRFCLAETSCPQEDNYPNGLCIKVNGKLFPLPGFAPPPKNGVEQKRPGRPLNITSLVRLSSAVPNQIVVTWAPEIGKTYSMSVYLVRQLTSPLLLQRLRMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTVPCRAVTCSHLQCFDAALYLQMNEKKPTWICPVCDKKATYESLIIDGLFMEILND--CTDVDEIQFQEDGTWCPMRPKKETLKLSS 437          

HSP 2 Score: 49.6766 bits (117), Expect = 9.046e-5
Identity = 34/72 (47.22%), Postives = 53/72 (73.61%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL-NNEMPRPGIGI 70
            MAD+E ++ MV +FR+SELQ L+ +AG++KSGRK +L +RAL+L+R   S+ +++KI+EL     PR   G+
Sbjct:    1 MADIEELRNMVSSFRVSELQVLLGFAGRNKSGRKHELLLRALHLLRSGCSSAVQIKIKELYRRRYPRTFEGL 72          

BLAST of EMLSAG00000000339 vs. GO
Match: - (symbol:Pias1 "Protein inhibitor of activated STAT 1 (Predicted)" species:10116 "Rattus norvegicus" [GO:0000122 "negative regulation of transcription from RNA polymerase II promoter" evidence=IEA] [GO:0003676 "nucleic acid binding" evidence=IEA] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0016605 "PML body" evidence=IEA] [GO:0019789 "SUMO ligase activity" evidence=IEA] [GO:0031625 "ubiquitin protein ligase binding" evidence=IEA] [GO:0032436 "positive regulation of proteasomal ubiquitin-dependent protein catabolic process" evidence=IEA] [GO:0033235 "positive regulation of protein sumoylation" evidence=IEA] [GO:0042127 "regulation of cell proliferation" evidence=IEA] InterPro:IPR003034 InterPro:IPR004181 InterPro:IPR027227 Pfam:PF02891 PROSITE:PS50800 PROSITE:PS51044 SMART:SM00513 RGD:1307843 GO:GO:0005634 GO:GO:0045893 GO:GO:0008152 GO:GO:0016605 GO:GO:0008270 GO:GO:0007283 GO:GO:0003676 GO:GO:0042127 GO:GO:0000122 Gene3D:1.10.720.30 GO:GO:0033235 GO:GO:0032436 EMBL:CH473975 GO:GO:0019789 GO:GO:0065004 GeneTree:ENSGT00550000074410 InterPro:IPR023321 Pfam:PF14324 PROSITE:PS51466 OrthoDB:EOG7HF1JB TreeFam:TF323787 GO:GO:0051152 PANTHER:PTHR10782:SF11 OMA:LRENHGH UniGene:Rn.24369 EMBL:AABR06055250 Ensembl:ENSRNOT00000066009 NextBio:35584410 Uniprot:G3V9T0)

HSP 1 Score: 313.153 bits (801), Expect = 1.312e-94
Identity = 173/409 (42.30%), Postives = 253/409 (61.86%), Query Frame = 0

Query:  225 PHTKTVVPIQNSEPKYDIANNGHL---MYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATG-KLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEALPSKDEESSSSSQKPKSIQNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATTTNKASEDKIVECITLDDSDEESPANPPAKKT 627
            P +  ++P+    PK+++    HL   ++P  PD++L  LPFY +   L+KP+SL   +S RF+   F F LTP Q   I+ S   D +G K ++  Q+Q+RFCL+ETSCPQ+D+FP ++CV VN +  +LP  +P  K GVEPKRP +PINITS  +LS T+PN + V+WT E G+ + +A+Y V++L+S  L++ L+ KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LYIQMNE+KP W+CPVC+K A YE+L++DG F E+L   K  T+ +EI  K D  W  + SK E           +Q             E    Y+  D       +        ++NK  + ++++ +T+D S +E    PPAK+T
Sbjct:  103 PASSPLLPVSLLGPKHEL-ELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSS--MDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEIL---KYCTDCDEIQFKEDGSWAPMRSKKE-----------VQ-------------EVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEVID-LTIDSSSDEEEEEPPAKRT 480          

HSP 2 Score: 60.8474 bits (146), Expect = 3.212e-8
Identity = 29/60 (48.33%), Postives = 44/60 (73.33%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL 59
            MAD   ++ MVM+ R+SELQ L+ YAG++K GRK +L  +AL+L++   S  ++MKI+EL
Sbjct:    1 MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKEL 60          

BLAST of EMLSAG00000000339 vs. GO
Match: - (symbol:Pias1 "protein inhibitor of activated STAT 1" species:10090 "Mus musculus" [GO:0000122 "negative regulation of transcription from RNA polymerase II promoter" evidence=IMP] [GO:0003676 "nucleic acid binding" evidence=IEA] [GO:0003677 "DNA binding" evidence=IEA] [GO:0003714 "transcription corepressor activity" evidence=ISA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005622 "intracellular" evidence=ISA] [GO:0005634 "nucleus" evidence=ISO;IDA] [GO:0006351 "transcription, DNA-templated" evidence=IEA] [GO:0006355 "regulation of transcription, DNA-templated" evidence=IEA] [GO:0007259 "JAK-STAT cascade" evidence=ISA] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0016605 "PML body" evidence=IDA] [GO:0016874 "ligase activity" evidence=IEA] [GO:0016925 "protein sumoylation" evidence=IDA] [GO:0019789 "SUMO ligase activity" evidence=ISO] [GO:0019899 "enzyme binding" evidence=ISO] [GO:0019904 "protein domain specific binding" evidence=ISO] [GO:0031625 "ubiquitin protein ligase binding" evidence=IPI] [GO:0032436 "positive regulation of proteasomal ubiquitin-dependent protein catabolic process" evidence=IMP] [GO:0033235 "positive regulation of protein sumoylation" evidence=ISO] [GO:0042127 "regulation of cell proliferation" evidence=IMP] [GO:0045893 "positive regulation of transcription, DNA-templated" evidence=ISO] [GO:0046872 "metal ion binding" evidence=IEA] [GO:0051152 "positive regulation of smooth muscle cell differentiation" evidence=ISO] [GO:0065004 "protein-DNA complex assembly" evidence=ISO] [GO:2001141 "regulation of RNA biosynthetic process" evidence=ISA] InterPro:IPR003034 InterPro:IPR004181 InterPro:IPR027227 Pfam:PF02891 PROSITE:PS50800 PROSITE:PS51044 SMART:SM00513 UniPathway:UPA00886 EMBL:AF077950 MGI:MGI:1913125 GO:GO:0005634 GO:GO:0003714 GO:GO:0045893 GO:GO:0003677 GO:GO:0016607 GO:GO:0016605 GO:GO:0008270 GO:GO:0007283 GO:GO:0006351 GO:GO:0042127 GO:GO:0000122 Gene3D:1.10.720.30 GO:GO:0033235 GO:GO:0032436 GO:GO:0016925 GO:GO:0007259 GO:GO:0019789 GO:GO:0065004 eggNOG:NOG125513 InterPro:IPR023321 Pfam:PF14324 PROSITE:PS51466 CTD:8554 HOVERGEN:HBG053598 KO:K04706 OrthoDB:EOG7HF1JB TreeFam:TF323787 GO:GO:0051152 PANTHER:PTHR10782:SF11 EMBL:AK075708 EMBL:BC051417 RefSeq:NP_062637.2 UniGene:Mm.431253 UniGene:Mm.491651 ProteinModelPortal:O88907 SMR:O88907 BioGrid:208005 DIP:DIP-29277N IntAct:O88907 MINT:MINT-4107556 PhosphoSite:O88907 PaxDb:O88907 PRIDE:O88907 Ensembl:ENSMUST00000098651 GeneID:56469 KEGG:mmu:56469 UCSC:uc009qas.1 HOGENOM:HOG000230594 InParanoid:O88907 OMA:LRENHGH ChiTaRS:PIAS1 NextBio:312730 PRO:PR:O88907 ArrayExpress:O88907 Bgee:O88907 CleanEx:MM_PIAS1 Genevestigator:O88907 Uniprot:O88907)

HSP 1 Score: 312.768 bits (800), Expect = 1.340e-94
Identity = 173/409 (42.30%), Postives = 253/409 (61.86%), Query Frame = 0

Query:  225 PHTKTVVPIQNSEPKYDIANNGHL---MYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATG-KLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEALPSKDEESSSSSQKPKSIQNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATTTNKASEDKIVECITLDDSDEESPANPPAKKT 627
            P +  ++P+    PK+++    HL   ++P  PD++L  LPFY +   L+KP+SL   +S RF+   F F LTP Q   I+ S   D +G K ++  Q+Q+RFCL+ETSCPQ+D+FP ++CV VN +  +LP  +P  K GVEPKRP +PINITS  +LS T+PN + V+WT E G+ + +A+Y V++L+S  L++ L+ KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LYIQMNE+KP W+CPVC+K A YE+L++DG F E+L   K  T+ +EI  K D  W  + SK E           +Q             E    Y+  D       +        ++NK  + ++++ +T+D S +E    PPAK+T
Sbjct:  103 PASSPLLPVSLLGPKHEL-ELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSS--MDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEIL---KYCTDCDEIQFKEDGSWAPMRSKKE-----------VQ-------------EVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEVID-LTIDSSSDEEEEEPPAKRT 480          

HSP 2 Score: 60.8474 bits (146), Expect = 3.076e-8
Identity = 29/60 (48.33%), Postives = 44/60 (73.33%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL 59
            MAD   ++ MVM+ R+SELQ L+ YAG++K GRK +L  +AL+L++   S  ++MKI+EL
Sbjct:    1 MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKEL 60          

BLAST of EMLSAG00000000339 vs. GO
Match: - (symbol:Pias1 "protein inhibitor of activated STAT, 1" species:10116 "Rattus norvegicus" [GO:0000122 "negative regulation of transcription from RNA polymerase II promoter" evidence=IEA;ISO] [GO:0003676 "nucleic acid binding" evidence=IEA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005634 "nucleus" evidence=ISO;IDA] [GO:0007283 "spermatogenesis" evidence=IEP] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0016605 "PML body" evidence=IEA;ISO] [GO:0016925 "protein sumoylation" evidence=ISO] [GO:0019789 "SUMO ligase activity" evidence=IEA;ISO] [GO:0019899 "enzyme binding" evidence=ISO] [GO:0019904 "protein domain specific binding" evidence=IPI] [GO:0031625 "ubiquitin protein ligase binding" evidence=IEA;ISO] [GO:0032436 "positive regulation of proteasomal ubiquitin-dependent protein catabolic process" evidence=IEA;ISO] [GO:0033235 "positive regulation of protein sumoylation" evidence=IEA;ISO] [GO:0042127 "regulation of cell proliferation" evidence=IEA;ISO] [GO:0045893 "positive regulation of transcription, DNA-templated" evidence=IMP] [GO:0051152 "positive regulation of smooth muscle cell differentiation" evidence=IMP] [GO:0065004 "protein-DNA complex assembly" evidence=IMP] InterPro:IPR003034 InterPro:IPR004181 InterPro:IPR027227 Pfam:PF02891 PROSITE:PS50800 PROSITE:PS51044 SMART:SM00513 RGD:1307843 GO:GO:0005634 GO:GO:0045893 GO:GO:0008152 GO:GO:0008270 GO:GO:0007283 GO:GO:0003676 Gene3D:1.10.720.30 GO:GO:0019789 GO:GO:0065004 eggNOG:NOG125513 InterPro:IPR023321 Pfam:PF14324 PROSITE:PS51466 CTD:8554 HOVERGEN:HBG053598 KO:K04706 GO:GO:0051152 PANTHER:PTHR10782:SF11 HOGENOM:HOG000230594 EMBL:AY644724 RefSeq:NP_001100299.2 UniGene:Rn.24369 ProteinModelPortal:A9UK05 SMR:A9UK05 IntAct:A9UK05 MINT:MINT-6594236 STRING:10116.ENSRNOP00000059152 PhosphoSite:A9UK05 PaxDb:A9UK05 GeneID:300772 KEGG:rno:300772 NextBio:647483 Genevestigator:A9UK05 Uniprot:A9UK05)

HSP 1 Score: 312.383 bits (799), Expect = 2.165e-94
Identity = 173/409 (42.30%), Postives = 252/409 (61.61%), Query Frame = 0

Query:  225 PHTKTVVPIQNSEPKYDIANNGHL---MYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATG-KLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEALPSKDEESSSSSQKPKSIQNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATTTNKASEDKIVECITLDDSDEESPANPPAKKT 627
            P +  ++P+    PK+++    HL   ++P  PD++L  LPFY +   L+KP+SL   +S RF+   F F LTP Q   I  S   D +G K ++  Q+Q+RFCL+ETSCPQ+D+FP ++CV VN +  +LP  +P  K GVEPKRP +PINITS  +LS T+PN + V+WT E G+ + +A+Y V++L+S  L++ L+ KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LYIQMNE+KP W+CPVC+K A YE+L++DG F E+L   K  T+ +EI  K D  W  + SK E           +Q             E    Y+  D       +        ++NK  + ++++ +T+D S +E    PPAK+T
Sbjct:  103 PASSPLLPVSLLGPKHEL-ELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQIGSS--MDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEIL---KYCTDCDEIQFKEDGSWAPMRSKKE-----------VQ-------------EVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEVID-LTIDSSSDEEEEEPPAKRT 480          

HSP 2 Score: 60.8474 bits (146), Expect = 3.296e-8
Identity = 29/60 (48.33%), Postives = 44/60 (73.33%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL 59
            MAD   ++ MVM+ R+SELQ L+ YAG++K GRK +L  +AL+L++   S  ++MKI+EL
Sbjct:    1 MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKEL 60          

BLAST of EMLSAG00000000339 vs. GO
Match: - (symbol:PIAS1 "Uncharacterized protein" species:9823 "Sus scrofa" [GO:0003676 "nucleic acid binding" evidence=IEA] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0019789 "SUMO ligase activity" evidence=IEA] InterPro:IPR003034 InterPro:IPR004181 InterPro:IPR027227 Pfam:PF02891 PROSITE:PS50800 PROSITE:PS51044 SMART:SM00513 GO:GO:0008152 GO:GO:0008270 GO:GO:0003676 Gene3D:1.10.720.30 GO:GO:0019789 GeneTree:ENSGT00550000074410 InterPro:IPR023321 Pfam:PF14324 PROSITE:PS51466 OrthoDB:EOG7HF1JB TreeFam:TF323787 PANTHER:PTHR10782:SF11 OMA:LRENHGH EMBL:CU468716 EMBL:CU929738 EMBL:AEMK01129071 Ensembl:ENSSSCT00000005470 Uniprot:F1SJH4)

HSP 1 Score: 305.834 bits (782), Expect = 7.728e-93
Identity = 157/325 (48.31%), Postives = 223/325 (68.62%), Query Frame = 0

Query:  225 PHTKTVVPIQNSEPKYDIANNGHL---MYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATG-KLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEALPSKDE 543
            P +  ++P+    PK+++    HL   ++P  PD++L  LPFY +   L+KP+SL   +S RF+   F F LTP Q   I+ S   D +G K ++  Q+Q+RFCL+ETSCPQ+D+FP ++CV VN +  +LP  +P  K GVEPKRP +PINITS  +LS T+PN + V+ T E G+ + +A+Y V++L+S  L++ L+ KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LYIQMNE+KP W+CPVC+K A YE+L++DG F E+L   K  T+ +EI  K D  W  + SK E
Sbjct:  104 PASSPLLPVSLLGPKHEL-ELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSS--MDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVS-TAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEIL---KYCTDCDEIQFKEDGSWAPMRSKKE 421          

HSP 2 Score: 56.6102 bits (135), Expect = 5.279e-7
Identity = 26/53 (49.06%), Postives = 40/53 (75.47%), Query Frame = 0

Query:    8 QAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL 59
            + MVM+ R+SELQ L+ YAG++K GRK +L  +AL+L++   S  ++MKI+EL
Sbjct:    9 KQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKEL 61          

BLAST of EMLSAG00000000339 vs. C. finmarchicus
Match: gi|592822814|gb|GAXK01131754.1| (TSA: Calanus finmarchicus comp160021_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 399.823 bits (1026), Expect = 1.161e-129
Identity = 184/292 (63.01%), Postives = 224/292 (76.71%), Query Frame = 0

Query:  248 LMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKL-GTENEIVLKNDAEWEAL 538
            L+YP  PDV L  LPFYK+E +LLKP SL P+ + RFQ Q F F+LTP Q + I+ S YR+  GK EY+ Q+QMRF L ETSC Q+DNFP+SICV VNG+   LPN IPT KPG EPKRPPKPINIT  CKLS+T  NY+NV+W VE G+   +++Y VE LT   L+  LK KG R   YT+A+IK+KL D+D EIATTSCKVTLACPLGKMRM+ PCRA+TCDHLQCFDA+LY+QMNE+KPKW+CPVCNK A+ ENL+VDG+F E++  P+L   E+EIVL ND  W+ L
Sbjct:  329 LVYPTYPDVSLKKLPFYKIEEMLLKPCSLQPNGNGRFQEQNFTFYLTPGQTSEISNSSYRNEQGKPEYRKQVQMRFSLLETSCEQEDNFPSSICVKVNGKLCPLPNPIPTAKPGAEPKRPPKPINITPLCKLSSTTANYINVSWAVEVGRAHTISVYQVENLTYKNLLDQLKAKGQRQPDYTRALIKEKLADQDQEIATTSCKVTLACPLGKMRMNAPCRASTCDHLQCFDAQLYLQMNEKKPKWVCPVCNKAALVENLLVDGFFMELIKSPRLPADEHEIVLHNDGTWDPL 1204          

HSP 2 Score: 77.7962 bits (190), Expect = 4.295e-14
Identity = 36/63 (57.14%), Postives = 51/63 (80.95%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEM 63
            M++ E +Q MV+ FR+SELQ LM +AGKSK+G+K+DLQ RA  LI ++S+DI +KI+EL+N M
Sbjct:   26 MSENEKLQNMVLAFRMSELQCLMVFAGKSKAGKKTDLQERAEELISIDSSDINIKIKELSNSM 214          

BLAST of EMLSAG00000000339 vs. C. finmarchicus
Match: gi|592857038|gb|GAXK01100506.1| (TSA: Calanus finmarchicus comp7468_c2_seq1 transcribed RNA sequence)

HSP 1 Score: 397.512 bits (1020), Expect = 5.107e-122
Identity = 185/292 (63.36%), Postives = 225/292 (77.05%), Query Frame = 0

Query:  248 LMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGT-ENEIVLKNDAEWEAL 538
            L+YP  PDV L  LPFYK+E  LLKP SL P+ + RFQ Q F F+LTP Q++ I+ S YR   GK EY+ QIQMRF L ETSC Q+DNFP+SICV VNG+   LPN IPTNKPG EPKRPP+PINIT  CKLS+T  NY+NV+W VE G+   V++Y V+ LT   L+  LK KG R   YT+A+IK+KL D+D EIATTSCKVTLACPLGKMRM+ PCRA+TCDHLQCFDA+LY+QMNE+KPKW+CPVCNK A+ ENL+VDG+F E++  P+L + E+EIVL ND  W+ L
Sbjct: 1985 LVYPTYPDVSLKKLPFYKIEETLLKPCSLQPNGNGRFQEQNFTFYLTPGQSSDISNSSYRSEQGKPEYRKQIQMRFSLLETSCEQEDNFPSSICVKVNGKLCPLPNPIPTNKPGAEPKRPPRPINITPLCKLSSTTANYINVSWAVEVGRAHTVSVYQVDNLTYKDLLDQLKAKGQRQPDYTRALIKEKLADQDQEIATTSCKVTLACPLGKMRMNAPCRASTCDHLQCFDAQLYLQMNEKKPKWVCPVCNKPALVENLLVDGFFLELIRSPRLPSDEHEIVLHNDGTWDPL 2860          

HSP 2 Score: 75.0998 bits (183), Expect = 4.568e-13
Identity = 36/63 (57.14%), Postives = 49/63 (77.78%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEM 63
            M++ E  + MV  FR+SELQ LM +AG+SK+GRK+DLQ RA  L+RLNS+DI +KI+EL + M
Sbjct: 1682 MSENEKYRNMVDAFRMSELQNLMIFAGRSKAGRKTDLQARAKELVRLNSSDINIKIKELTSTM 1870          

BLAST of EMLSAG00000000339 vs. C. finmarchicus
Match: gi|592857035|gb|GAXK01100509.1| (TSA: Calanus finmarchicus comp7468_c2_seq4 transcribed RNA sequence)

HSP 1 Score: 389.423 bits (999), Expect = 5.396e-121
Identity = 183/286 (63.99%), Postives = 221/286 (77.27%), Query Frame = 0

Query:  248 LMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKL-GTENEIVLKND 532
            L+YP  PDV L  LPFYK+E  LLKP SL P+ + RFQ Q F F+LTP Q++ I+ S YR   GK EY+ QIQMRF L ETSC Q+DNFP+SICV VNG+   LPN IPTNKPG EPKRPP+PINIT  CKLS+T  NY+NV+W VE G+   V++Y V+ LT   L+  LK KG R   YT+A+IK+KL D+D EIATTSCKVTLACPLGKMRM+ PCRA+TCDHLQCFDA+LY+QMNE+KPKW+CPVCNK A+ ENL+VDG+F E++  P+L   E+EIVL ND
Sbjct: 1985 LVYPTYPDVSLKKLPFYKIEETLLKPCSLQPNGNGRFQEQNFTFYLTPGQSSDISNSSYRSEQGKPEYRKQIQMRFSLLETSCEQEDNFPSSICVKVNGKLCPLPNPIPTNKPGAEPKRPPRPINITPLCKLSSTTANYINVSWAVEVGRAHTVSVYQVDNLTYKDLLDQLKAKGQRQPDYTRALIKEKLADQDQEIATTSCKVTLACPLGKMRMNAPCRASTCDHLQCFDAQLYLQMNEKKPKWVCPVCNKPALVENLLVDGFFLELIRSPRLPSDEHEIVLHND 2842          

HSP 2 Score: 74.7146 bits (182), Expect = 6.210e-13
Identity = 36/63 (57.14%), Postives = 49/63 (77.78%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEM 63
            M++ E  + MV  FR+SELQ LM +AG+SK+GRK+DLQ RA  L+RLNS+DI +KI+EL + M
Sbjct: 1682 MSENEKYRNMVDAFRMSELQNLMIFAGRSKAGRKTDLQARAKELVRLNSSDINIKIKELTSTM 1870          

BLAST of EMLSAG00000000339 vs. C. finmarchicus
Match: gi|592857037|gb|GAXK01100507.1| (TSA: Calanus finmarchicus comp7468_c2_seq2 transcribed RNA sequence)

HSP 1 Score: 357.836 bits (917), Expect = 2.356e-107
Identity = 173/292 (59.25%), Postives = 213/292 (72.95%), Query Frame = 0

Query:  248 LMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKL-GTENEIVLKNDAEWEAL 538
            L+YP  PDV L  LPFYK+E  LLKP SL P+ + RFQ Q F F+LTP Q++ I+ S YR   GK EY+ QIQMRF L ET+C Q DNFP+S+ V VNG+  +LPNII  NK      RP  P+NITS CKLS T  N + V+W VE G+   V++Y V+ LT   L+  LK KG R   YT+A+IK+KL D+D EIATTSCKVTLACPLGKMRM+ PCRA+TCDHLQCFDA+LY+QMNE+KPKW+CPVCNK A+ ENL+VDG+F E++  P+L   E+EIVL ND  W+ L
Sbjct: 1985 LVYPTYPDVSLKKLPFYKIEETLLKPCSLQPNGNGRFQEQNFTFYLTPGQSSDISNSSYRSEQGKPEYRKQIQMRFSLLETTCDQLDNFPSSVSVKVNGKMCSLPNII-LNKTV----RPSLPVNITSLCKLSPTTSNTVTVSWAVEVGRAHTVSVYQVDNLTYKDLLDQLKAKGQRQPDYTRALIKEKLADQDQEIATTSCKVTLACPLGKMRMNAPCRASTCDHLQCFDAQLYLQMNEKKPKWVCPVCNKPALVENLLVDGFFLELIRSPRLPSDEHEIVLHNDGTWDPL 2845          

HSP 2 Score: 75.0998 bits (183), Expect = 5.143e-13
Identity = 36/63 (57.14%), Postives = 49/63 (77.78%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEM 63
            M++ E  + MV  FR+SELQ LM +AG+SK+GRK+DLQ RA  L+RLNS+DI +KI+EL + M
Sbjct: 1682 MSENEKYRNMVDAFRMSELQNLMIFAGRSKAGRKTDLQARAKELVRLNSSDINIKIKELTSTM 1870          

BLAST of EMLSAG00000000339 vs. C. finmarchicus
Match: gi|592857036|gb|GAXK01100508.1| (TSA: Calanus finmarchicus comp7468_c2_seq3 transcribed RNA sequence)

HSP 1 Score: 352.443 bits (903), Expect = 1.863e-106
Identity = 171/292 (58.56%), Postives = 213/292 (72.95%), Query Frame = 0

Query:  248 LMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGT-ENEIVLKNDAEWEAL 538
            L+YP  PDV L  LPFYK+E  LLKP SL P+ + RFQ Q F F+LTP Q++ I+ S YR   GK EY+ QIQMRF L ET+C Q DNFP+S+ V VNG+  +LPNII  NK      RP  P+NITS CKLS T  N + V+W VE G+   +++Y VE LT   L+  LK KG R   YTKA+IKDKL+D+D EIATTSCKVTLACPLGKMRM+ P RA+TCDHLQCFDA+LY+ MNE+KPKW+CPVCNK A+ ENL++DG+F +++  P+L T E+EIVL ND  W+ +
Sbjct: 1985 LVYPTYPDVSLKKLPFYKIEETLLKPCSLQPNGNGRFQEQNFTFYLTPGQSSDISNSSYRSEQGKPEYRKQIQMRFSLLETTCDQLDNFPSSVSVKVNGKMCSLPNII-LNKTV----RPSLPVNITSLCKLSPTTSNTVTVSWAVEVGRAHTISVYQVENLTHKDLLDQLKMKGLRQPDYTKALIKDKLSDQDQEIATTSCKVTLACPLGKMRMTAPARASTCDHLQCFDAQLYLMMNEKKPKWVCPVCNKPALMENLLIDGFFLDLVRSPRLPTDEHEIVLHNDGTWDPI 2845          

HSP 2 Score: 74.7146 bits (182), Expect = 5.994e-13
Identity = 36/63 (57.14%), Postives = 49/63 (77.78%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEM 63
            M++ E  + MV  FR+SELQ LM +AG+SK+GRK+DLQ RA  L+RLNS+DI +KI+EL + M
Sbjct: 1682 MSENEKYRNMVDAFRMSELQNLMIFAGRSKAGRKTDLQARAKELVRLNSSDINIKIKELTSTM 1870          

BLAST of EMLSAG00000000339 vs. C. finmarchicus
Match: gi|592762724|gb|GAXK01191689.1| (TSA: Calanus finmarchicus comp40955_c2_seq1 transcribed RNA sequence)

HSP 1 Score: 322.013 bits (824), Expect = 4.271e-101
Identity = 159/285 (55.79%), Postives = 195/285 (68.42%), Query Frame = 0

Query:  255 DVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKL-GTENEIVLKNDAEWEAL 538
            +V +  LPFY+    LL PS L+   + RFQ   + F LT  QAN IA +    AT K+ Y  Q+Q+RFC  +T+  Q D FP SICV VNG+   LPN IPTNKP VEPKRPPKPI++T  CKLS TLPN +NV W  + GKG+ + I  VEKL+S  L++ L  KG R S +T+ +IK KLND   EIATT+ KVT+ACPLGKMRMS PCR TTCDHLQCFDA L++ MNERKP W CPVC+  A+YENLMVDGYF +V+   +L   ENEI+L  D  W  +
Sbjct:  222 NVTIKKLPFYEQHGALLPPSVLHAKGTGRFQEAQYQFVLTVQQANDIASNRDIRATTKVNYVYQVQLRFCPLDTTKEQGDEFPPSICVQVNGKMCPLPNPIPTNKPNVEPKRPPKPIDLTPLCKLSPTLPNTVNVKWAADQGKGWVIVIDLVEKLSSEQLLERLVQKGTRQSEFTRELIKKKLNDEGDEIATTNLKVTVACPLGKMRMSSPCRPTTCDHLQCFDASLFLMMNERKPTWQCPVCDSAALYENLMVDGYFLDVIKSKELPEEENEIILNQDGSWHPI 1076          

BLAST of EMLSAG00000000339 vs. C. finmarchicus
Match: gi|592857034|gb|GAXK01100510.1| (TSA: Calanus finmarchicus comp7468_c2_seq5 transcribed RNA sequence)

HSP 1 Score: 335.109 bits (858), Expect = 4.692e-101
Identity = 158/246 (64.23%), Postives = 188/246 (76.42%), Query Frame = 0

Query:  248 LMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWI 493
            L+YP  PDV L  LPFYK+E  LLKP SL P+ + RFQ Q F F+LTP Q++ I+ S YR   GK EY+ QIQMRF L ETSC Q+DNFP+SICV VNG+   LPN IPTNKPG EPKRPP+PINIT  CKLS+T  NY+NV+W VE G+   +++Y VE LT   L+  LK KG R   YTKA+IKDKL+D+D EIATTSCKVTLACPLGKMRM+ P RA+TCDHLQCFDA+LY+ MNE+K KW+
Sbjct: 1985 LVYPTYPDVSLKKLPFYKIEETLLKPCSLQPNGNGRFQEQNFTFYLTPGQSSDISNSSYRSEQGKPEYRKQIQMRFSLLETSCEQEDNFPSSICVKVNGKLCPLPNPIPTNKPGAEPKRPPRPINITPLCKLSSTTANYINVSWAVEVGRAHTISVYQVENLTHKDLLDQLKMKGLRQPDYTKALIKDKLSDQDQEIATTSCKVTLACPLGKMRMTAPARASTCDHLQCFDAQLYLMMNEKKTKWV 2722          

HSP 2 Score: 74.7146 bits (182), Expect = 5.679e-13
Identity = 36/63 (57.14%), Postives = 49/63 (77.78%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEM 63
            M++ E  + MV  FR+SELQ LM +AG+SK+GRK+DLQ RA  L+RLNS+DI +KI+EL + M
Sbjct: 1682 MSENEKYRNMVDAFRMSELQNLMIFAGRSKAGRKTDLQARAKELVRLNSSDINIKIKELTSTM 1870          

BLAST of EMLSAG00000000339 vs. C. finmarchicus
Match: gi|592846005|gb|GAXK01111539.1| (TSA: Calanus finmarchicus comp52362_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 308.916 bits (790), Expect = 6.642e-92
Identity = 151/282 (53.55%), Postives = 193/282 (68.44%), Query Frame = 0

Query:  255 DVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKL-GTENEIVLKNDAEW 535
            +V+   LPFY +   LLKP+SL    S+RFQ   F F L+P QA  IA +       KL+Y  QIQ+RFC  + S  Q D FP SICV VNG+   LPN IPTNKP VEPKRPP+P++IT  CKL+ TL N ++V W  + GK + V I+ VEKL+S+ L++ L  KG R+  +T+ +IK KL D +  IATT+ KVT++CPLGKMRMS PCR TTCDHLQCFDA L++ MNE+KP WICPVC+  A Y+++MVDGYF EV+   +L   ENEI+L  D  W
Sbjct: 1044 NVKFKKLPFYDIHGELLKPTSLVTQGSSRFQEAQFQFLLSPEQATNIASNRDIRMGSKLDYLYQIQLRFCQLDPSAEQGDEFPPSICVQVNGKMCTLPNPIPTNKPNVEPKRPPRPVHITPLCKLTPTLNNIVSVKWAADYGKNWVVGIWLVEKLSSSQLLERLSTKGKRNPDFTRELIKKKLTDDEDGIATTNLKVTVSCPLGKMRMSTPCRPTTCDHLQCFDASLFLMMNEKKPTWICPVCDSPAKYDDIMVDGYFEEVIKSAELPEEENEIILNQDGSW 1889          

HSP 2 Score: 65.0846 bits (157), Expect = 4.916e-10
Identity = 27/55 (49.09%), Postives = 45/55 (81.82%), Query Frame = 0

Query:    5 EMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIREL 59
            E ++ M++ FR+SELQ L+ +AG+++SG+K++LQ RAL L++LNS  ++ +IREL
Sbjct: 2139 EALRNMILTFRVSELQMLLGFAGRNQSGKKTELQDRALELLKLNSTPVQKRIREL 2303          

BLAST of EMLSAG00000000339 vs. C. finmarchicus
Match: gi|592857033|gb|GAXK01100511.1| (TSA: Calanus finmarchicus comp7468_c2_seq6 transcribed RNA sequence)

HSP 1 Score: 295.434 bits (755), Expect = 1.698e-86
Identity = 146/246 (59.35%), Postives = 177/246 (71.95%), Query Frame = 0

Query:  248 LMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWI 493
            L+YP  PDV L  LPFYK+E  LLKP SL P+ + RFQ Q F F+LTP Q++ I+ S YR   GK EY+ QIQMRF L ET+C Q DNFP+S+ V VNG+  +LPNII  NK      RP  P+NITS CKLS T  N + V+W VE G+   +++Y VE LT   L+  LK KG R   YTKA+IKDKL+D+D EIATTSCKVTLACPLGKMRM+ P RA+TCDHLQCFDA+LY+ MNE+K KW+
Sbjct: 1985 LVYPTYPDVSLKKLPFYKIEETLLKPCSLQPNGNGRFQEQNFTFYLTPGQSSDISNSSYRSEQGKPEYRKQIQMRFSLLETTCDQLDNFPSSVSVKVNGKMCSLPNII-LNKTV----RPSLPVNITSLCKLSPTTSNTVTVSWAVEVGRAHTISVYQVENLTHKDLLDQLKMKGLRQPDYTKALIKDKLSDQDQEIATTSCKVTLACPLGKMRMTAPARASTCDHLQCFDAQLYLMMNEKKTKWV 2707          

HSP 2 Score: 74.7146 bits (182), Expect = 6.067e-13
Identity = 36/63 (57.14%), Postives = 49/63 (77.78%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEM 63
            M++ E  + MV  FR+SELQ LM +AG+SK+GRK+DLQ RA  L+RLNS+DI +KI+EL + M
Sbjct: 1682 MSENEKYRNMVDAFRMSELQNLMIFAGRSKAGRKTDLQARAKELVRLNSSDINIKIKELTSTM 1870          

BLAST of EMLSAG00000000339 vs. C. finmarchicus
Match: gi|592892101|gb|GAXK01066274.1| (TSA: Calanus finmarchicus comp5263_c8_seq1 transcribed RNA sequence)

HSP 1 Score: 289.656 bits (740), Expect = 4.736e-86
Identity = 146/301 (48.50%), Postives = 198/301 (65.78%), Query Frame = 0

Query:  243 ANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNI--IPTNKPGVEPKRPPKPINITSNCKL-SATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGT--ENEIVLKNDAEWEAL 538
            AN G L +P   DV++  LPFY V+A LL+P +++  D+   Q Q   F+LT  QAN +      +  G + YK  I +RF        QDD+FP ++C+ VN +   LPN   +P N+P +E KRPPKP+ ITS CKL + +  N + VTW+        V+IY VEKLT   L+K LK +G R    TKA+I+ KL D+D EI+TTSCKV++ACPLG  RM+LPCRA+TCDHLQCFDA LY++MNE+KPKW+CPVCNK A YE+L +DG++ ++L  PK  +   N+I+L NDA WE +
Sbjct:  672 ANPGRLSFP---DVKIRDLPFYPVKATLLRPCAISAKDNQDTQQQNLAFYLTQDQANTVNNGRKVETNGMVVYKMHILLRFTKMSADQVQDDDFPKNLCLKVNNKICPLPNPKPVPANRPNMEAKRPPKPLIITSLCKLNTKSCLNQVAVTWSPNKDVQHTVSIYLVEKLTPVDLLKHLKTRGQRDPQITKAVIQSKLEDKDDEISTTSCKVSMACPLGMARMTLPCRASTCDHLQCFDADLYLKMNEKKPKWVCPVCNKAAYYEHLFLDGFYIQLLLSPKFKSIATNDIILNNDASWEPV 1565          

BLAST of EMLSAG00000000339 vs. L. salmonis peptides
Match: EMLSAP00000000339 (pep:novel supercontig:LSalAtl2s:LSalAtl2s104:636256:638695:-1 gene:EMLSAG00000000339 transcript:EMLSAT00000000339 description:"maker-LSalAtl2s104-augustus-gene-6.22")

HSP 1 Score: 1396.72 bits (3614), Expect = 0.000e+0
Identity = 686/686 (100.00%), Postives = 686/686 (100.00%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEMPRPGIGIGNNNKESPLNQLLGERSTRSRKGESDNPSSNALEEAPVTRRGARSTRTRVSYAEPDEDETSEVLSDSSXDESEESDKGYNPPSEEEEVVESGGEDTDSAIPLSPSHSPTRLHSKKSANLPNILKKSPRGHISNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEESSSSSQKPKSIQNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATTTNKASEDKIVECITLDDSDEESPANPPAKKTRYDNEYAESCIPISSDTGXLXSSSSFSSNDSNXQPQSLSNQKSINKEPPALXCLSEDED 686
            MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEMPRPGIGIGNNNKESPLNQLLGERSTRSRKGESDNPSSNALEEAPVTRRGARSTRTRVSYAEPDEDETSEVLSDSSXDESEESDKGYNPPSEEEEVVESGGEDTDSAIPLSPSHSPTRLHSKKSANLPNILKKSPRGHISNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEESSSSSQKPKSIQNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATTTNKASEDKIVECITLDDSDEESPANPPAKKTRYDNEYAESCIPISSDTGXLXSSSSFSSNDSNXQPQSLSNQKSINKEPPALXCLSEDED
Sbjct:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEMPRPGIGIGNNNKESPLNQLLGERSTRSRKGESDNPSSNALEEAPVTRRGARSTRTRVSYAEPDEDETSEVLSDSSXDESEESDKGYNPPSEEEEVVESGGEDTDSAIPLSPSHSPTRLHSKKSANLPNILKKSPRGHISNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEESSSSSQKPKSIQNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATTTNKASEDKIVECITLDDSDEESPANPPAKKTRYDNEYAESCIPISSDTGXLXSSSSFSSNDSNXQPQSLSNQKSINKEPPALXCLSEDED 686          

BLAST of EMLSAG00000000339 vs. L. salmonis peptides
Match: EMLSAP00000009591 (pep:novel supercontig:LSalAtl2s:LSalAtl2s617:113952:116969:-1 gene:EMLSAG00000009591 transcript:EMLSAT00000009591 description:"maker-LSalAtl2s617-augustus-gene-1.39")

HSP 1 Score: 311.227 bits (796), Expect = 2.606e-96
Identity = 164/332 (49.40%), Postives = 211/332 (63.55%), Query Frame = 0

Query:  254 PDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFC-LN-ETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKL-GTENEIVLKNDAEWEALPSKDEESSSSSQKPK----SIQNPIIAPPVNNTSVETVDVYDDE 578
            PDV+LV LPFY V A LL PSSL    + R Q   F F +TPSQA  IA       + KL+Y  QIQ+RFC L+ ++S    D FP  I + VNG+   LPN IPTN+ GVEPKRPPKP+NITS CKLS  LPN +N+ W  E GK + V I+ V K TS  L++ LK KG R+S YTK  I  K+ D D ++ T   +V++ACPLGKMRM LPCR +TC HLQCFDA L++QMNERKP W CPVC+  A++++LM+DGYF +V+N   L   EN+I+L  D  W  +P ++E + +S+ K K    +    +   P N  S  T    DDE
Sbjct:  204 PDVKLVKLPFYDVIAELLXPSSLVTQGNNRHQEAQFQFFMTPSQATDIASHRDIRISSKLDYLYQIQLRFCPLSLDSSKEMADEFPPGIILQVNGKGVVLPNPIPTNRTGVEPKRPPKPVNITSCCKLSPILPNVVNIKWAGEYGKWWVVGIWLVMKCTSEDLLERLKKKGTRNSEYTKKTIIAKMCDDD-DVETECLRVSVACPLGKMRMXLPCRPSTCGHLQCFDASLFLQMNERKPTWNCPVCDSKALFDDLMIDGYFMDVINSQDLPKDENDIILNGDGSWMPVPKEEESAKTSTSKEKKNXSNSDETVNGGPSNGNSTSTPSTNDDE 534          

HSP 2 Score: 63.5438 bits (153), Expect = 1.141e-10
Identity = 26/59 (44.07%), Postives = 46/59 (77.97%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIREL 59
            + + E ++ M++ FR+SELQ L+ +AG++K+G+K +LQ RAL L+++ S+ ++ KIREL
Sbjct:    4 LQEQESLKHMILTFRVSELQXLLGFAGRNKNGKKHELQGRALELVKIRSSPMQAKIREL 62          

BLAST of EMLSAG00000000339 vs. SwissProt
Match: gi|56404326|sp|Q6AZ28.1|PIAS2_RAT (RecName: Full=E3 SUMO-protein ligase PIAS2; AltName: Full=Androgen receptor-interacting protein 3; Short=ARIP3; AltName: Full=DAB2-interacting protein; Short=DIP; AltName: Full=Msx-interacting-zinc finger protein; AltName: Full=Protein inhibitor of activated STAT x; AltName: Full=Protein inhibitor of activated STAT2)

HSP 1 Score: 318.161 bits (814), Expect = 7.235e-99
Identity = 171/397 (43.07%), Postives = 244/397 (61.46%), Query Frame = 0

Query:  231 VPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEESSSSSQKPKSIQNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATTTNKASEDKIVECITLDDSDEESPANPPAKK 626
            V +Q+++P +++      + P  PDVQL +LPFY V  +L+KP+SL  S   RFQ + F+F LTP Q   I  S      G+ +Y  Q+Q+R CL ETSCPQ+DN+P S+C+ VNG+   LP   P  K G+E KRP +P+NITS  +LS+ +PN ++++W  E GK + +++Y V +LTS  L++ LK KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LY+QMNE+KP WICPVC+K A YE+L++DG F E+LND      +EI  + D  W  +  K E    +SQ                T VE+  V+          +K  S+  A+  +K   D +++      SDEE   +PPAK+
Sbjct:  121 VLLQDTKPTFEMQQPSPPIPPVHPDVQLKTLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILND--CSDVDEIKFQEDGSWCPMRPKKEAMKVTSQP--------------CTKVESSSVF----------SKPCSVTVASDASKKKID-VIDLTIESSSDEEE--DPPAKR 488          

HSP 2 Score: 66.6254 bits (161), Expect = 3.770e-10
Identity = 34/74 (45.95%), Postives = 51/74 (68.92%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSN-DIKMKIREL-NNEMPRPGIGIGN 72
            MAD E ++ MV +FR+SELQ L+ +AG++KSGRK DL +RAL+L++      +++KIREL     PR   G+ +
Sbjct:    1 MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCTPAVQIKIRELYRRRYPRTLEGLSD 74          

BLAST of EMLSAG00000000339 vs. SwissProt
Match: gi|229463031|sp|O75928.3|PIAS2_HUMAN (RecName: Full=E3 SUMO-protein ligase PIAS2; AltName: Full=Androgen receptor-interacting protein 3; Short=ARIP3; AltName: Full=DAB2-interacting protein; Short=DIP; AltName: Full=Msx-interacting zinc finger protein; Short=Miz1; AltName: Full=PIAS-NY protein; AltName: Full=Protein inhibitor of activated STAT x; AltName: Full=Protein inhibitor of activated STAT2)

HSP 1 Score: 316.62 bits (810), Expect = 8.033e-98
Identity = 163/353 (46.18%), Postives = 231/353 (65.44%), Query Frame = 0

Query:  231 VPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEESSSSSQKPKSIQ-NPIIAPPVNNT-----SVETVDVYD 576
            V +Q+++P +++      + P  PDVQL +LPFY V  +L+KP+SL  S   RFQ + F+F LTP Q   I  S      G+ +Y  Q+Q+R CL ETSCPQ+DN+P S+C+ VNG+   LP   P  K G+E KRP +P+NITS  +LS+ +PN ++++W  E GK + +++Y V +LTS  L++ LK KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LY+QMNE+KP WICPVC+K A YE+L++DG F E+LND      +EI  + D  W  +  K E    SSQ    I+ + +++ P + T     S + VDV D
Sbjct:  121 VLLQDTKPTFEMQQPSPPIPPVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILND--CSDVDEIKFQEDGSWCPMRPKKEAMKVSSQPCTKIESSSVLSKPCSVTVASEASKKKVDVID 471          

HSP 2 Score: 67.0106 bits (162), Expect = 2.800e-10
Identity = 35/74 (47.30%), Postives = 52/74 (70.27%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL-NNEMPRPGIGIGN 72
            MAD E ++ MV +FR+SELQ L+ +AG++KSGRK DL +RAL+L++   S  +++KIREL     PR   G+ +
Sbjct:    1 MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLEGLSD 74          

BLAST of EMLSAG00000000339 vs. SwissProt
Match: gi|20138891|sp|O75925.2|PIAS1_HUMAN (RecName: Full=E3 SUMO-protein ligase PIAS1; AltName: Full=DEAD/H box-binding protein 1; AltName: Full=Gu-binding protein; Short=GBP; AltName: Full=Protein inhibitor of activated STAT protein 1; AltName: Full=RNA helicase II-binding protein)

HSP 1 Score: 317.39 bits (812), Expect = 9.104e-98
Identity = 158/325 (48.62%), Postives = 224/325 (68.92%), Query Frame = 0

Query:  225 PHTKTVVPIQNSEPKYDIANNGHL---MYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATG-KLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEALPSKDE 543
            P +  ++P+    PK+++    HL   ++P  PD++L  LPFY +   L+KP+SL   +S RF+   F F LTP Q   I+ S   D +G K ++  Q+Q+RFCL+ETSCPQ+D+FP ++CV VN +  +LP  +P  K GVEPKRP +PINITS  +LS T+PN + V+WT E G+ + +A+Y V++L+S  L++ L+ KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LYIQMNE+KP W+CPVC+K A YE+L++DG F E+L   K  T+ +EI  K D  W  + SK E
Sbjct:  103 PASSPLLPVSLLGPKHEL-ELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSS--MDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEIL---KYCTDCDEIQFKEDGTWAPMRSKKE 421          

HSP 2 Score: 60.8474 bits (146), Expect = 2.541e-8
Identity = 29/60 (48.33%), Postives = 44/60 (73.33%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL 59
            MAD   ++ MVM+ R+SELQ L+ YAG++K GRK +L  +AL+L++   S  ++MKI+EL
Sbjct:    1 MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKEL 60          

BLAST of EMLSAG00000000339 vs. SwissProt
Match: gi|56404605|sp|Q8C5D8.2|PIAS2_MOUSE (RecName: Full=E3 SUMO-protein ligase PIAS2; AltName: Full=Androgen receptor-interacting protein 3; Short=ARIP3; AltName: Full=DAB2-interacting protein; Short=DIP; AltName: Full=Msx-interacting zinc finger protein; AltName: Full=Protein inhibitor of activated STAT x; AltName: Full=Protein inhibitor of activated STAT2; AltName: Full=RING-type E3 ubiquitin transferase PIAS2)

HSP 1 Score: 316.62 bits (810), Expect = 1.091e-97
Identity = 171/397 (43.07%), Postives = 244/397 (61.46%), Query Frame = 0

Query:  231 VPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEESSSSSQKPKSIQNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATTTNKASEDKIVECITLDDSDEESPANPPAKK 626
            V +Q+++P +++      + P  PDVQL +LPFY V  +L+KP+SL  S   RFQ + F+F LTP Q   I  S      G+ +Y  Q+Q+R CL ETSCPQ+DN+P S+C+ VNG+   LP   P  K G+E KRP +P+NITS  +LS+ +PN ++++W  E GK + +++Y V +LTS  L++ LK KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LY+QMNE+KP WICPVC+K A YE+L++DG F E+LND      +EI  + D  W  +  K E    +SQ                T VE+  V+          +K  S+  A+  +K   D +++      SDEE   +PPAK+
Sbjct:  121 VLLQDTKPTFEMQQPSPPIPPVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILND--CSDVDEIKFQEDGSWCPMRPKKEAMKVTSQP--------------CTKVESSSVF----------SKPCSVTVASDASKKKID-VIDLTIESSSDEEE--DPPAKR 488          

HSP 2 Score: 66.6254 bits (161), Expect = 3.187e-10
Identity = 32/60 (53.33%), Postives = 47/60 (78.33%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL 59
            MAD E ++ MV +FR+SELQ L+ +AG++KSGRK DL +RAL+L++   S  +++KIREL
Sbjct:    1 MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIREL 60          

BLAST of EMLSAG00000000339 vs. SwissProt
Match: gi|56405305|sp|O88907.2|PIAS1_MOUSE (RecName: Full=E3 SUMO-protein ligase PIAS1; AltName: Full=DEAD/H box-binding protein 1; AltName: Full=Protein inhibitor of activated STAT protein 1; AltName: Full=RING-type E3 ubiquitin transferase PIAS1)

HSP 1 Score: 312.768 bits (800), Expect = 4.994e-96
Identity = 173/409 (42.30%), Postives = 253/409 (61.86%), Query Frame = 0

Query:  225 PHTKTVVPIQNSEPKYDIANNGHL---MYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATG-KLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEALPSKDEESSSSSQKPKSIQNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATTTNKASEDKIVECITLDDSDEESPANPPAKKT 627
            P +  ++P+    PK+++    HL   ++P  PD++L  LPFY +   L+KP+SL   +S RF+   F F LTP Q   I+ S   D +G K ++  Q+Q+RFCL+ETSCPQ+D+FP ++CV VN +  +LP  +P  K GVEPKRP +PINITS  +LS T+PN + V+WT E G+ + +A+Y V++L+S  L++ L+ KG R+  +++A+IK+KL  D D EIATTS +V+L CPLGKMR+++PCRA TC HLQCFDA LYIQMNE+KP W+CPVC+K A YE+L++DG F E+L   K  T+ +EI  K D  W  + SK E           +Q             E    Y+  D       +        ++NK  + ++++ +T+D S +E    PPAK+T
Sbjct:  103 PASSPLLPVSLLGPKHEL-ELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSS--MDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRTYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEIL---KYCTDCDEIQFKEDGSWAPMRSKKE-----------VQ-------------EVTASYNGVDGCLSSTLEHQVASHNQSSNKNKKVEVID-LTIDSSSDEEEEEPPAKRT 480          

HSP 2 Score: 60.8474 bits (146), Expect = 2.413e-8
Identity = 29/60 (48.33%), Postives = 44/60 (73.33%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLN-SNDIKMKIREL 59
            MAD   ++ MVM+ R+SELQ L+ YAG++K GRK +L  +AL+L++   S  ++MKI+EL
Sbjct:    1 MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKEL 60          

BLAST of EMLSAG00000000339 vs. SwissProt
Match: gi|56404322|sp|O70260.2|PIAS3_RAT (RecName: Full=E3 SUMO-protein ligase PIAS3; AltName: Full=KChAP; AltName: Full=Potassium channel-associated protein; AltName: Full=Protein inhibitor of activated STAT protein 3)

HSP 1 Score: 300.056 bits (767), Expect = 2.180e-91
Identity = 154/345 (44.64%), Postives = 215/345 (62.32%), Query Frame = 0

Query:  202 SNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEES 545
            S  GS + ++S+ PT+ T   L+             PK ++  +  L  P  PDV +  LPFY+V   L++P++L  + S RF+   F F LTP Q   I  S       K +Y  Q+Q+RFCL ETSCPQ+D FP ++ V VNG+   LP  +P  K G EPKRP +PINIT   +LSAT+PN + V W+ E G+ + +++Y V +LT+ TL++ L+ KG R+  +++A+IK+KL  D D E+ATTS +V+L CPLGKMR+++PCRA TC HLQ FDA LY+QMNE+KP W CPVC+K A YE+L++DG F E+LN       +EI    D  W  +  K E S
Sbjct:   85 SPVGSPSPLASIPPTLLTPGTLL------------GPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNS--CSDCDEIQFMEDGSWCPMKPKKEAS 415          

BLAST of EMLSAG00000000339 vs. SwissProt
Match: gi|56405302|sp|O54714.3|PIAS3_MOUSE (RecName: Full=E3 SUMO-protein ligase PIAS3; AltName: Full=Protein inhibitor of activated STAT protein 3)

HSP 1 Score: 297.36 bits (760), Expect = 2.342e-90
Identity = 153/345 (44.35%), Postives = 213/345 (61.74%), Query Frame = 0

Query:  202 SNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEES 545
            S  GS   ++ + PT+ T   L+             PK ++  +  L  P  PDV +  LPFY+V   L++P++L  + S RF+   F F LTP Q   I  S       K +Y  Q+Q+RFCL ETSCPQ+D FP ++ V VNG+   LP  +P  K G EPKRP +PINIT   +LSAT+PN + V W+ E G+ + +++Y V +LT+ TL++ L+ KG R+  +++A+IK+KL  D D E+ATTS +V+L CPLGKMR+++PCRA TC HLQ FDA LY+QMNE+KP W CPVC+K A YE+L++DG F E+LN       +EI    D  W  +  K E S
Sbjct:   85 SPVGSPGPLAPIPPTLLTPGTLL------------GPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNS--CSDCDEIQFMEDGSWCPMKPKKEAS 415          

BLAST of EMLSAG00000000339 vs. SwissProt
Match: gi|56405390|sp|Q9Y6X2.2|PIAS3_HUMAN (RecName: Full=E3 SUMO-protein ligase PIAS3; AltName: Full=Protein inhibitor of activated STAT protein 3)

HSP 1 Score: 294.664 bits (753), Expect = 2.877e-89
Identity = 145/309 (46.93%), Postives = 201/309 (65.05%), Query Frame = 0

Query:  238 PKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEES 545
            PK ++  +  L  P  PDV +  LPFY+V   L++P++L  + S RF+   F F LTP Q   I  S       K +Y  Q+Q+RFCL ETSCPQ+D FP ++ V VNG+   LP  +P  K G EPKRP +PINIT   +LSAT+PN + V W+ E G+ + +++Y V +LT+ TL++ L+ KG R+  +++A+IK+KL  D D E+ATTS +V+L CPLGKMR+++PCRA TC HLQ FDA LY+QMNE+KP W CPVC+K A YE+L++DG F E+L+       +EI    D  W  +  K E S
Sbjct:  109 PKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQVQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILSS--CSDCDEIQFMEDGSWCPMKPKKEAS 415          

BLAST of EMLSAG00000000339 vs. SwissProt
Match: gi|34922847|sp|Q9JM05.2|PIAS4_MOUSE (RecName: Full=E3 SUMO-protein ligase PIAS4; AltName: Full=PIASy; AltName: Full=Protein inhibitor of activated STAT protein 4; AltName: Full=Protein inhibitor of activated STAT protein gamma; Short=PIAS-gamma; AltName: Full=RING-type E3 ubiquitin transferase PIAS4)

HSP 1 Score: 248.825 bits (634), Expect = 2.240e-73
Identity = 133/300 (44.33%), Postives = 186/300 (62.00%), Query Frame = 0

Query:  254 PDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEESSSSSQKP 552
            P+V+LV LPF+ +   LLKP+ L P  + + Q    +F LTP Q   I  S       K     Q+ +R C ++TSCPQ+D +P +I V VN    ++P   P+NKPGVEPKRP +PIN+T    LS+   N + VTW    GK + VA+Y V +LTS+ L++ LK  G +H    KA++K+KL  D D EIATT  +V+L CPL KMR+S+PCRA TC HLQCFDA  Y+QMNE+KP W+CPVC+K A Y+ L++DG   ++L++ +    +EI    +  W  +   ++E S S Q P
Sbjct:  122 PEVRLVKLPFFNMLDELLKPTELVPQSAEKLQESPCIFALTPRQVEMIRNSRELQPGVK---AVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSAT-NRITVTWG-NYGKSYSVALYLVRQLTSSDLLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAAYDQLIIDGLLSKILSECE--GADEIEFLAEGSWRPI-RAEKEPSCSPQGP 413          

BLAST of EMLSAG00000000339 vs. SwissProt
Match: gi|34922831|sp|Q8N2W9.1|PIAS4_HUMAN (RecName: Full=E3 SUMO-protein ligase PIAS4; AltName: Full=PIASy; AltName: Full=Protein inhibitor of activated STAT protein 4; AltName: Full=Protein inhibitor of activated STAT protein gamma; Short=PIAS-gamma; AltName: Full=RING-type E3 ubiquitin transferase PIAS4)

HSP 1 Score: 247.669 bits (631), Expect = 6.836e-73
Identity = 132/295 (44.75%), Postives = 186/295 (63.05%), Query Frame = 0

Query:  254 PDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEALPSKDEESSS 547
            P+V+LV LPF+ +   LLKP+ L P ++ + Q    +F LTP Q   I  S       K     Q+ +R C ++TSCPQ+D +P +I V VN    ++P   P+NKPGVEPKRP +PIN+T    LS+   N + VTW    GK + VA+Y V +LTS+ L++ LK  G +H    KA++K+KL  D D EIATT  +V+L CPL KMR+S+PCRA TC HLQCFDA  Y+QMNE+KP W+CPVC+K A Y+ L++DG   ++L++ +   E E ++  D  W  + ++ E S S
Sbjct:  129 PEVRLVKLPFFNMLDELLKPTELVPQNNEKLQESPCIFALTPRQVELIRNSRELQPGVK---AVQVVLRICYSDTSCPQEDQYPPNIAVKVNHSYCSVPGYYPSNKPGVEPKRPCRPINLTHLMYLSSAT-NRITVTWG-NYGKSYSVALYLVRQLTSSELLQRLKTIGVKHPELCKALVKEKLRLDPDSEIATTGVRVSLICPLVKMRLSVPCRAETCAHLQCFDAVFYLQMNEKKPTWMCPVCDKPAPYDQLIIDGLLSKILSECEDADEIEYLV--DGSWCPIRAEKERSCS 416          

BLAST of EMLSAG00000000339 vs. Select Arthropod Genomes
Match: XP_006561119.1 (PREDICTED: E3 SUMO-protein ligase PIAS3 isoform X3 [Apis mellifera])

HSP 1 Score: 361.303 bits (926), Expect = 8.382e-117
Identity = 176/291 (60.48%), Postives = 213/291 (73.20%), Query Frame = 0

Query:  250 YPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEAL 538
            YP  PDV+L  LPF+ + A LLKPSSL P  S R Q  TF+FHLTP Q+  +A S    A  K++Y  QIQMRFCL ETSC Q+D FP SI V VNG+   LPN IPTNKPGVEPKRPP+P+NI+   KLS T+ N ++VTW+ + G+ + +AIY V KL+S  L+  LK +G RHS YT+ +IK+KLN D D EIATTS +V+LACPLGKMRMS PCRATTC HLQCFDA L++QMNERKP W CPVC+K A+Y+NL++DGYF EVLN  KL  + NEI L  D  WE L
Sbjct:  101 YPVHPDVRLKKLPFFDLLAELLKPSSLMPQGSMRLQENTFMFHLTPQQSTDVASSRDCRAGSKMDYTVQIQMRFCLQETSCEQEDCFPPSIAVKVNGKLCPLPNPIPTNKPGVEPKRPPRPVNISPLVKLSPTVANQIHVTWSADYGRRYAIAIYLVRKLSSAELLSRLKSRGVRHSDYTRGLIKEKLNEDADSEIATTSLRVSLACPLGKMRMSTPCRATTCSHLQCFDASLFLQMNERKPTWNCPVCDKPALYDNLVIDGYFQEVLNSKKLLPDVNEIQLLQDGSWENL 391          

BLAST of EMLSAG00000000339 vs. Select Arthropod Genomes
Match: XP_623571.3 (PREDICTED: E3 SUMO-protein ligase PIAS3 isoform X1 [Apis mellifera])

HSP 1 Score: 362.459 bits (929), Expect = 2.347e-116
Identity = 176/291 (60.48%), Postives = 213/291 (73.20%), Query Frame = 0

Query:  250 YPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEAL 538
            YP  PDV+L  LPF+ + A LLKPSSL P  S R Q  TF+FHLTP Q+  +A S    A  K++Y  QIQMRFCL ETSC Q+D FP SI V VNG+   LPN IPTNKPGVEPKRPP+P+NI+   KLS T+ N ++VTW+ + G+ + +AIY V KL+S  L+  LK +G RHS YT+ +IK+KLN D D EIATTS +V+LACPLGKMRMS PCRATTC HLQCFDA L++QMNERKP W CPVC+K A+Y+NL++DGYF EVLN  KL  + NEI L  D  WE L
Sbjct:  173 YPVHPDVRLKKLPFFDLLAELLKPSSLMPQGSMRLQENTFMFHLTPQQSTDVASSRDCRAGSKMDYTVQIQMRFCLQETSCEQEDCFPPSIAVKVNGKLCPLPNPIPTNKPGVEPKRPPRPVNISPLVKLSPTVANQIHVTWSADYGRRYAIAIYLVRKLSSAELLSRLKSRGVRHSDYTRGLIKEKLNEDADSEIATTSLRVSLACPLGKMRMSTPCRATTCSHLQCFDASLFLQMNERKPTWNCPVCDKPALYDNLVIDGYFQEVLNSKKLLPDVNEIQLLQDGSWENL 463          

HSP 2 Score: 73.1738 bits (178), Expect = 1.405e-12
Identity = 32/59 (54.24%), Postives = 50/59 (84.75%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIREL 59
            MA+ + ++ M+++FR+SELQ L+ +AG++KSGRK++LQ RAL L+RL S+ I++KIREL
Sbjct:    1 MAETKELENMILSFRVSELQMLLGFAGRNKSGRKNELQGRALELLRLRSHPIQLKIREL 59          

BLAST of EMLSAG00000000339 vs. Select Arthropod Genomes
Match: XP_006561118.1 (PREDICTED: E3 SUMO-protein ligase PIAS3 isoform X2 [Apis mellifera])

HSP 1 Score: 362.073 bits (928), Expect = 2.701e-116
Identity = 176/291 (60.48%), Postives = 213/291 (73.20%), Query Frame = 0

Query:  250 YPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEAL 538
            YP  PDV+L  LPF+ + A LLKPSSL P  S R Q  TF+FHLTP Q+  +A S    A  K++Y  QIQMRFCL ETSC Q+D FP SI V VNG+   LPN IPTNKPGVEPKRPP+P+NI+   KLS T+ N ++VTW+ + G+ + +AIY V KL+S  L+  LK +G RHS YT+ +IK+KLN D D EIATTS +V+LACPLGKMRMS PCRATTC HLQCFDA L++QMNERKP W CPVC+K A+Y+NL++DGYF EVLN  KL  + NEI L  D  WE L
Sbjct:  164 YPVHPDVRLKKLPFFDLLAELLKPSSLMPQGSMRLQENTFMFHLTPQQSTDVASSRDCRAGSKMDYTVQIQMRFCLQETSCEQEDCFPPSIAVKVNGKLCPLPNPIPTNKPGVEPKRPPRPVNISPLVKLSPTVANQIHVTWSADYGRRYAIAIYLVRKLSSAELLSRLKSRGVRHSDYTRGLIKEKLNEDADSEIATTSLRVSLACPLGKMRMSTPCRATTCSHLQCFDASLFLQMNERKPTWNCPVCDKPALYDNLVIDGYFQEVLNSKKLLPDVNEIQLLQDGSWENL 454          

HSP 2 Score: 70.0922 bits (170), Expect = 1.325e-11
Identity = 30/50 (60.00%), Postives = 44/50 (88.00%), Query Frame = 0

Query:   10 MVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIREL 59
            M+++FR+SELQ L+ +AG++KSGRK++LQ RAL L+RL S+ I++KIREL
Sbjct:    1 MILSFRVSELQMLLGFAGRNKSGRKNELQGRALELLRLRSHPIQLKIREL 50          

BLAST of EMLSAG00000000339 vs. Select Arthropod Genomes
Match: EFX76934.1 (hypothetical protein DAPPUDRAFT_213657 [Daphnia pulex])

HSP 1 Score: 358.992 bits (920), Expect = 1.085e-114
Identity = 177/335 (52.84%), Postives = 232/335 (69.25%), Query Frame = 0

Query:  250 YPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDAT--GKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVE-AGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEALPSKDEESSSSSQKPKSIQNPIIAPPVN--NTSVETVDVYDDE 578
            +P  PDV+L  LPF+   A LL+PSSL P+ ++R+    FVFHLTP QA  IA S  RDA    K+++ NQIQMRFCL ETSC Q+DNFP+++ V VNG+   LPN IPTNKPGVEPKRPP+P+NITS C+LS T+ N +NVTW+ +  G+G+ +A+Y V +L S  L++ L+ KG R + YT+ +I+DKL D D EIATTS KV+L CPLGKMRM LPCRAT+C HLQCFDA L++QMNERKP W+CPVC+K  +Y+ L +DGYF ++LN P L  +  E+ L  D  W  L +K E   +++       + +   P N  N  VET+D  D E
Sbjct:  141 FPVHPDVRLKKLPFFDSIAELLRPSSLVPTGNSRYHDTNFVFHLTPQQATDIASS--RDARPGSKIDFLNQIQMRFCLLETSCEQEDNFPSNLSVKVNGKLVTLPNPIPTNKPGVEPKRPPRPVNITSLCRLSPTVANQINVTWSSDMGGRGYVLAVYVVRRLASTDLLQRLRTKGVRAAEYTRGLIRDKLEDADAEIATTSLKVSLVCPLGKMRMQLPCRATSCSHLQCFDASLFLQMNERKPTWVCPVCDKPILYDQLAIDGYFSDILNSPLLPVDIMEVQLNVDGTWTVLTTKKETKKNANADSSVNLDVVEELPSNPTNPKVETIDEGDLE 473          

HSP 2 Score: 77.7962 bits (190), Expect = 4.909e-14
Identity = 34/77 (44.16%), Postives = 58/77 (75.32%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEMPRPGIGIGNNNKES 77
            M++ E+++ MVM+FR+SEL  L+++AG+S+SGRK++LQ+RAL LI+L S+ I  K+REL   + +  + +G +  E+
Sbjct:    1 MSETEVLKNMVMSFRVSELTMLLSFAGRSRSGRKTELQMRALELIKLRSSPINAKVRELYRSIQQQTVAMGQSAAET 77          

BLAST of EMLSAG00000000339 vs. Select Arthropod Genomes
Match: EAA09481.4 (AGAP005031-PB, partial [Anopheles gambiae str. PEST])

HSP 1 Score: 344.354 bits (882), Expect = 7.102e-109
Identity = 166/293 (56.66%), Postives = 214/293 (73.04%), Query Frame = 0

Query:  246 GHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSA-RFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTEN-EIVLKNDAEW 535
            G+  YP  PDVQL  L F+ V A LLKP++L PS++A R Q  ++ FHLTP QAN IA +     + K+E+  Q+Q+RFCL ET+C QDD+FP +I + VN +   LPN IPTNKPGVEPKRPP+P+NIT+  KLS T+ N+++V+W  E  +G+ VA Y V KL+S  L++ LK KG + + YT+A+IK+KLN D DCEIATT  KV+L CPLGKMRM+ PCR++TC HLQCFDA LY+QMNERKP W CPVC+K A+Y NL++DGYF EVL   KL +E+ EI L  D  W
Sbjct:  157 GYGDYPIHPDVQLKKLAFFDVMATLLKPATLVPSNTAQRVQEGSYFFHLTPQQANEIALNRDISNSAKIEHNVQVQLRFCLLETTCEQDDHFPPNIVLKVNNKLCPLPNPIPTNKPGVEPKRPPRPVNITAQVKLSPTVANHISVSWCTEYNRGYAVACYLVRKLSSAQLLQRLKTKGVKAADYTRALIKEKLNEDADCEIATTMLKVSLICPLGKMRMTTPCRSSTCSHLQCFDASLYLQMNERKPTWNCPVCDKPAVYNNLVIDGYFQEVLVSTKLSSEDTEIQLHKDGSW 449          

BLAST of EMLSAG00000000339 vs. Select Arthropod Genomes
Match: EDO64052.1 (AGAP005031-PA, partial [Anopheles gambiae str. PEST])

HSP 1 Score: 345.125 bits (884), Expect = 1.130e-108
Identity = 166/293 (56.66%), Postives = 214/293 (73.04%), Query Frame = 0

Query:  246 GHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSA-RFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTEN-EIVLKNDAEW 535
            G+  YP  PDVQL  L F+ V A LLKP++L PS++A R Q  ++ FHLTP QAN IA +     + K+E+  Q+Q+RFCL ET+C QDD+FP +I + VN +   LPN IPTNKPGVEPKRPP+P+NIT+  KLS T+ N+++V+W  E  +G+ VA Y V KL+S  L++ LK KG + + YT+A+IK+KLN D DCEIATT  KV+L CPLGKMRM+ PCR++TC HLQCFDA LY+QMNERKP W CPVC+K A+Y NL++DGYF EVL   KL +E+ EI L  D  W
Sbjct:  157 GYGDYPIHPDVQLKKLAFFDVMATLLKPATLVPSNTAQRVQEGSYFFHLTPQQANEIALNRDISNSAKIEHNVQVQLRFCLLETTCEQDDHFPPNIVLKVNNKLCPLPNPIPTNKPGVEPKRPPRPVNITAQVKLSPTVANHISVSWCTEYNRGYAVACYLVRKLSSAQLLQRLKTKGVKAADYTRALIKEKLNEDADCEIATTMLKVSLICPLGKMRMTTPCRSSTCSHLQCFDASLYLQMNERKPTWNCPVCDKPAVYNNLVIDGYFQEVLVSTKLSSEDTEIQLHKDGSW 449          

BLAST of EMLSAG00000000339 vs. Select Arthropod Genomes
Match: gb|EFA06716.1| (E3 SUMO-protein ligase PIAS1-like Protein [Tribolium castaneum])

HSP 1 Score: 329.331 bits (843), Expect = 5.072e-103
Identity = 179/386 (46.37%), Postives = 240/386 (62.18%), Query Frame = 0

Query:  156 EEVVESGGEDTDSAIPLSPSHSPTRLHSKKSANLPNILKKSPRG----HISNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEW 535
            + + + GG  + S +P +PS SP      K  N+P      PR     H  N  S+ + S+  P++    +  P+ +     Q S+     ANN    +P  PDV+   LPF+ + + LLKPS+L P  + R Q  TF FHLTP QA  IA S       K +Y  Q+Q+RFCL ET+C QDD FP ++ V VN +   LPN IPTNKPGVEPKRPP+P+NIT   KLS  + N + V+W  + G+G+ +++  V+KLTS+ L++ LK KG +HS YT+ +IK+KL  D D EI TT  +V+L CPLGKMRM+ PCRA TC+HLQCFD+ L++QMNERKP W CPVC+K A+Y+NL +DGYF EVL  P L  + NEI L  D  W
Sbjct:   62 KSIQQQGG--SLSNVP-TPSLSP----GSKDHNMPQPQGTVPRTTATVHPYNIDSRQL-STRQPSLYNQSVYNPYPQYTQ--QKSQQGSIAANN----FPIHPDVKFRRLPFFDIISDLLKPSTLMPQSNQRMQEATFYFHLTPQQATDIASSRDIRPGVKCDYVKQVQLRFCLLETTCEQDDFFPPNVIVKVNNKPCPLPNPIPTNKPGVEPKRPPRPVNITPMVKLSPIVANMITVSWAPDYGRGYAISVALVQKLTSSDLLQRLKAKGPKHSDYTRGLIKEKLKEDADSEITTTCLRVSLICPLGKMRMTTPCRAITCNHLQCFDSSLFLQMNERKPTWTCPVCDKPALYDNLTIDGYFQEVLASPNLPPDLNEIQLHKDGSW 433          

HSP 2 Score: 67.781 bits (164), Expect = 6.786e-11
Identity = 30/70 (42.86%), Postives = 51/70 (72.86%), Query Frame = 0

Query:    3 DLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEMPRPGIGIGN 72
            +++ ++ M++ FR+SELQ L+ +AG++KSGRK++LQ RAL L+R+ S+ I  KIR+L   + + G  + N
Sbjct:    4 NIDELRNMLITFRVSELQMLLGFAGRNKSGRKTELQQRALELLRVRSHPIHQKIRDLYKSIQQQGGSLSN 73          

BLAST of EMLSAG00000000339 vs. Select Arthropod Genomes
Match: AHN56026.1 (suppressor of variegation 2-10, isoform N [Drosophila melanogaster])

HSP 1 Score: 324.709 bits (831), Expect = 1.038e-101
Identity = 168/360 (46.67%), Postives = 228/360 (63.33%), Query Frame = 0

Query:  178 PTRLHSKKSANLPNILKKSPRGHISNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTEN-EIVLKNDAEW 535
            PTR++S     LP  ++ +P G + +     +    +  +  A  L  HT ++              N  L  P  PDV+L  L FY V   L+KPS+L P ++ R Q   F F LTP QA  IA +     + K+E+  Q+Q+RFCL ETSC Q+D FP ++ V VN +   LPN+IPTN+P VEPKRPP+P+N+TSN KLS T+ N + V W  +  + +C+A+Y V+KLTS  L++ +K KG + + YT+ +IK+KL  D DCEIATT  KV+L CPLGKM+M LPCRA+TC HLQCFDA LY+QMNERKP W CPVC+K A+Y+NL++DGYF EVL    L +++ EI L  D  W
Sbjct:  114 PTRMYSH--IQLPPTVQPNPVGLVGSGQGVQVPGGQMNVVGGAPFL--HTHSI--------------NSQL--PIHPDVRLKKLAFYDVLGTLIKPSTLVPRNTQRVQEVPFYFTLTPQQATEIASNRDIRNSSKVEHAIQVQLRFCLVETSCDQEDCFPPNVNVKVNNKLCQLPNVIPTNRPNVEPKRPPRPVNVTSNVKLSPTVTNTITVQWCPDYTRSYCLAVYLVKKLTSTQLLQRMKTKGVKPADYTRGLIKEKLTEDADCEIATTMLKVSLNCPLGKMKMLLPCRASTCSHLQCFDASLYLQMNERKPTWNCPVCDKPAIYDNLVIDGYFQEVLGSSLLKSDDTEIQLHQDGSW 453          

BLAST of EMLSAG00000000339 vs. Select Arthropod Genomes
Match: AAG22293.1 (suppressor of variegation 2-10, isoform B [Drosophila melanogaster])

HSP 1 Score: 325.094 bits (832), Expect = 1.401e-101
Identity = 168/360 (46.67%), Postives = 229/360 (63.61%), Query Frame = 0

Query:  178 PTRLHSKKSANLPNILKKSPRGHISNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTEN-EIVLKNDAEW 535
            PTR++S     LP  ++ +P G + +     +    +  +  A  L  HT ++    NS+             P  PDV+L  L FY V   L+KPS+L P ++ R Q   F F LTP QA  IA +     + K+E+  Q+Q+RFCL ETSC Q+D FP ++ V VN +   LPN+IPTN+P VEPKRPP+P+N+TSN KLS T+ N + V W  +  + +C+A+Y V+KLTS  L++ +K KG + + YT+ +IK+KL  D DCEIATT  KV+L CPLGKM+M LPCRA+TC HLQCFDA LY+QMNERKP W CPVC+K A+Y+NL++DGYF EVL    L +++ EI L  D  W
Sbjct:   67 PTRMYSH--IQLPPTVQPNPVGLVGSGQGVQVPGGQMNVVGGAPFL--HTHSI----NSQ------------LPIHPDVRLKKLAFYDVLGTLIKPSTLVPRNTQRVQEVPFYFTLTPQQATEIASNRDIRNSSKVEHAIQVQLRFCLVETSCDQEDCFPPNVNVKVNNKLCQLPNVIPTNRPNVEPKRPPRPVNVTSNVKLSPTVTNTITVQWCPDYTRSYCLAVYLVKKLTSTQLLQRMKTKGVKPADYTRGLIKEKLTEDADCEIATTMLKVSLNCPLGKMKMLLPCRASTCSHLQCFDASLYLQMNERKPTWNCPVCDKPAIYDNLVIDGYFQEVLGSSLLKSDDTEIQLHQDGSW 406          

BLAST of EMLSAG00000000339 vs. Select Arthropod Genomes
Match: AFH07970.1 (suppressor of variegation 2-10, isoform M [Drosophila melanogaster])

HSP 1 Score: 324.324 bits (830), Expect = 1.455e-101
Identity = 168/360 (46.67%), Postives = 228/360 (63.33%), Query Frame = 0

Query:  178 PTRLHSKKSANLPNILKKSPRGHISNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTEN-EIVLKNDAEW 535
            PTR++S     LP  ++ +P G + +     +    +  +  A  L  HT ++              N  L  P  PDV+L  L FY V   L+KPS+L P ++ R Q   F F LTP QA  IA +     + K+E+  Q+Q+RFCL ETSC Q+D FP ++ V VN +   LPN+IPTN+P VEPKRPP+P+N+TSN KLS T+ N + V W  +  + +C+A+Y V+KLTS  L++ +K KG + + YT+ +IK+KL  D DCEIATT  KV+L CPLGKM+M LPCRA+TC HLQCFDA LY+QMNERKP W CPVC+K A+Y+NL++DGYF EVL    L +++ EI L  D  W
Sbjct:  114 PTRMYSH--IQLPPTVQPNPVGLVGSGQGVQVPGGQMNVVGGAPFL--HTHSI--------------NSQL--PIHPDVRLKKLAFYDVLGTLIKPSTLVPRNTQRVQEVPFYFTLTPQQATEIASNRDIRNSSKVEHAIQVQLRFCLVETSCDQEDCFPPNVNVKVNNKLCQLPNVIPTNRPNVEPKRPPRPVNVTSNVKLSPTVTNTITVQWCPDYTRSYCLAVYLVKKLTSTQLLQRMKTKGVKPADYTRGLIKEKLTEDADCEIATTMLKVSLNCPLGKMKMLLPCRASTCSHLQCFDASLYLQMNERKPTWNCPVCDKPAIYDNLVIDGYFQEVLGSSLLKSDDTEIQLHQDGSW 453          

BLAST of EMLSAG00000000339 vs. nr
Match: gi|665793292|ref|XP_008544285.1| (PREDICTED: E3 SUMO-protein ligase PIAS4 [Microplitis demolitor])

HSP 1 Score: 410.609 bits (1054), Expect = 3.887e-132
Identity = 256/624 (41.03%), Postives = 345/624 (55.29%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEMPRPGI------GIGNNNKESPLNQLLGERSTRSRKGESDNPSSNALEEAPVTRRGARSTRTRVSYAEPDEDETSEVLSDSSXDESEESDKGYNPPSEEEEVVESGGEDTDSAIPLSPSHSPTRLHSKKSANLPNILKKSPRGHISNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEALPSKDEESSSSSQKPKSI--------------QNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATTTNKASE 602
            MAD + ++ MV++FR+SELQ L+ +AG++KSGRK++LQ RAL L+RL S+ +++KIREL   +    +      G    + E  ++Q L +RS  SR+    NPS             AR+ R R +  +P         S SS D          PP+ +  + +S  + T SA    P+                        + S T  ++  S   P    + +L P      PIQ  +            YP  PDV+L  LPFY +   LLKPSSL P  S R Q  TF+FHLTP QAN IA S    A  +++Y  Q+QMRFCL ETSC Q+D FP  + V VNG+   LPN IPTNK GVEPKRPP+P+NI+   KLS T+ N + +TW+ + G+ + VA+Y V KLTS+ L+  LK +G RHS YT+ +IK+KLN D D EIATTS +V+LACPLGKMRMS PCR++TC HLQCFDA L++QMNERKP W CPVC+K A+Y++L++DGYF EVL   KL  + NEI L  D  WE L SK E     S K  S                N +++ P   ++     +  DE+D+     K +S K A    K S+
Sbjct:    1 MADTDELENMVLSFRVSELQMLLGFAGRNKSGRKNELQTRALELLRLRSHPVQLKIRELYKTIQADQLAAHQMYGQSGASNEPSIDQNLHQRSDDSRQNH--NPS------------WARAMRARPTQQQPSPT------SVSSKDL---------PPAHQASMPQS--QVTRSAAAYQPT-----------------------TYNSVTAQRSNTSVYQPYPYPSKVLPP------PIQIQQSS---------QYPIHPDVRLKKLPFYDLLGELLKPSSLMPQGSIRIQDNTFLFHLTPQQANDIANSRDCRAGSRMDYTVQVQMRFCLQETSCEQEDYFPPGVGVKVNGKLCPLPNPIPTNKSGVEPKRPPRPVNISPLVKLSPTVSNQIQITWSADYGRRYAVAVYLVRKLTSSELLNRLKARGARHSDYTRGLIKEKLNEDADSEIATTSLRVSLACPLGKMRMSTPCRSSTCSHLQCFDASLFLQMNERKPTWNCPVCDKPALYDHLVIDGYFQEVLLSNKLLPDVNEIQLLQDGSWENLVSKKERDKEKSDKSDSKARAKIDVDTVDLDESNLVVSTPEKKSAPVIDLISSDEEDDVENDYKTSSKKGANALPKKSQ 555          

BLAST of EMLSAG00000000339 vs. nr
Match: gi|646708456|gb|KDR14768.1| (E3 SUMO-protein ligase PIAS1 [Zootermopsis nevadensis])

HSP 1 Score: 375.17 bits (962), Expect = 1.254e-117
Identity = 190/367 (51.77%), Postives = 240/367 (65.40%), Query Frame = 0

Query:  250 YPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKL-NDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEALPSKDEESSSSSQKPK----------SIQNPIIAPPVNNTSVETVDVYD----DEDDEAVKAAKAASLKSATTTNKA 600
            YP  PDV+   LPF+ +   LLKPSSL P  + R Q   FVFHLTP QA  IA S       K+EY  Q+QMRFCL ETSC QDD FP  ICV VNG+   LPN IPTNKPGVEPKRPP+P+NIT+  KLS T+ N++ V+W  E G+G+ +A+Y V KLTS+ L++ LK +G RHS +T+ +IK+KL  D DCEIATTS +V+L CPLGKMRMS PCRA+TC HLQCFDA LY+QMNERKP W+CPVC+K A+Y+NL++DGYF +VL   KL T+ NEI L  D  W +L  K E++   S  P+          S +  +I PP      +   V D    D +DEA    K  +     ++N+A
Sbjct:  152 YPVHPDVRFKHLPFFDLMGELLKPSSLVPQGTQRLQEGNFVFHLTPQQATDIASSRDLRPGTKMEYAIQVQMRFCLLETSCEQDDFFPPGICVKVNGKMCPLPNPIPTNKPGVEPKRPPRPVNITTMVKLSPTVANHITVSWNTEYGRGYAIAVYLVRKLTSSELLQRLKTRGVRHSDFTRGLIKEKLAEDADCEIATTSLRVSLVCPLGKMRMSTPCRASTCYHLQCFDASLYLQMNERKPTWMCPVCDKPALYDNLVIDGYFQQVLLSGKLSTDGNEIQLHQDGSWSSLIVKKEQTRVLSPVPQEKPVVKIETLSDELEVIQPPRKEEPKKKATVIDLTLSDSEDEAEAPVKTNATNKPDSSNQA 518          

HSP 2 Score: 68.1662 bits (165), Expect = 2.635e-8
Identity = 28/50 (56.00%), Postives = 43/50 (86.00%), Query Frame = 0

Query:   10 MVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIREL 59
            MV++FR+SELQ L+ +AG++KSGRK++LQ RA+ L+RL S  ++MKI++L
Sbjct:    1 MVLSFRVSELQMLLGFAGRNKSGRKTELQARAVELLRLRSTPVQMKIKDL 50          

BLAST of EMLSAG00000000339 vs. nr
Match: gi|951579586|ref|XP_014487438.1| (PREDICTED: E3 SUMO-protein ligase PIAS2 isoform X2 [Dinoponera quadriceps])

HSP 1 Score: 372.089 bits (954), Expect = 8.847e-117
Identity = 222/549 (40.44%), Postives = 306/549 (55.74%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEMPRPGIGIGNNNKESPLNQLLGERSTRSRKGESDNPSSNALEEAPVTRRGARSTRTRVSYAEP----DEDETSEVLSDSSXDESEESD-----KGYNPPSEEEEVVESGGEDTDSAIPLSPSHSPTRLHSKKSANLPNILKKSPRGHISNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEAL 538
            MA+ E ++ M+++FR+SELQ L+ +AG++KSGRK               N+++ +  EL +    P I +        + +L     T     ++D  +         T    RS+ +   Y +P    D+    ++ S +      + D     + Y    +     +       +   L+P+H          A++P   + +P     +TG   + +   P+   A    P      P+Q  +P           YP  PDV+L  LPF+ +   LLKPSSL P  S R Q  TF+FHLTP QA  IA S    A  K++Y  Q+QMRFCL ETSC Q+D FP +I V VN +   LPN IPTNKPGVEPKRPP+P+NI+   KLS T+ N + V+W+ + G+ F VA+Y V KLTS  L+  LK++G RHS YT+ +IK+KLN D D EIATTS +V+LACPLGKMRM  PCRA+TC HLQCFDA L++QMNERKP W CPVC+K+A+Y+NL++DGYF EVLN  KL  + NEI L  D  WE L
Sbjct:    1 MAENEELENMILSFRVSELQMLLGFAGRNKSGRK---------------NELQARALELLHLRSHPAIRLK-------IRELYKTIHTSYSIHDADRTAFIYKSRYSGTSLYVRSSNSSCRYDKPYVRQDQMAAHQIYSQTGSSTEPQIDQTMHNRNYYSTRQAMSQQQQSQASVSAGKELTPAHQ---------ASIPQAPRTNPV--YQSTGYTNVTAQRAPSAGYAYSPYPPKVLPSPLQ-MQPA--------TQYPVHPDVRLKKLPFFDLLGELLKPSSLMPQGSLRLQENTFMFHLTPQQATDIASSRDCRAGSKMDYIVQVQMRFCLQETSCEQEDYFPPNITVKVNSKLCPLPNPIPTNKPGVEPKRPPRPVNISPLVKLSPTVGNEIRVSWSADYGRRFAVAVYLVRKLTSAELLSRLKNRGARHSDYTRGLIKEKLNEDADSEIATTSLRVSLACPLGKMRMCTPCRASTCSHLQCFDASLFLQMNERKPTWNCPVCDKSALYDNLVIDGYFQEVLNSNKLLPDVNEIQLLQDGSWENL 507          

BLAST of EMLSAG00000000339 vs. nr
Match: gi|951579589|ref|XP_014487440.1| (PREDICTED: E3 SUMO-protein ligase PIAS1 isoform X3 [Dinoponera quadriceps] >gi|951579591|ref|XP_014487441.1| PREDICTED: E3 SUMO-protein ligase PIAS1 isoform X3 [Dinoponera quadriceps])

HSP 1 Score: 367.851 bits (943), Expect = 2.724e-115
Identity = 219/540 (40.56%), Postives = 300/540 (55.56%), Query Frame = 0

Query:   10 MVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEMPRPGIGIGNNNKESPLNQLLGERSTRSRKGESDNPSSNALEEAPVTRRGARSTRTRVSYAEP----DEDETSEVLSDSSXDESEESD-----KGYNPPSEEEEVVESGGEDTDSAIPLSPSHSPTRLHSKKSANLPNILKKSPRGHISNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEAL 538
            M+++FR+SELQ L+ +AG++KSGRK               N+++ +  EL +    P I +        + +L     T     ++D  +         T    RS+ +   Y +P    D+    ++ S +      + D     + Y    +     +       +   L+P+H          A++P   + +P     +TG   + +   P+   A    P      P+Q  +P           YP  PDV+L  LPF+ +   LLKPSSL P  S R Q  TF+FHLTP QA  IA S    A  K++Y  Q+QMRFCL ETSC Q+D FP +I V VN +   LPN IPTNKPGVEPKRPP+P+NI+   KLS T+ N + V+W+ + G+ F VA+Y V KLTS  L+  LK++G RHS YT+ +IK+KLN D D EIATTS +V+LACPLGKMRM  PCRA+TC HLQCFDA L++QMNERKP W CPVC+K+A+Y+NL++DGYF EVLN  KL  + NEI L  D  WE L
Sbjct:    1 MILSFRVSELQMLLGFAGRNKSGRK---------------NELQARALELLHLRSHPAIRLK-------IRELYKTIHTSYSIHDADRTAFIYKSRYSGTSLYVRSSNSSCRYDKPYVRQDQMAAHQIYSQTGSSTEPQIDQTMHNRNYYSTRQAMSQQQQSQASVSAGKELTPAHQ---------ASIPQAPRTNPV--YQSTGYTNVTAQRAPSAGYAYSPYPPKVLPSPLQ-MQPA--------TQYPVHPDVRLKKLPFFDLLGELLKPSSLMPQGSLRLQENTFMFHLTPQQATDIASSRDCRAGSKMDYIVQVQMRFCLQETSCEQEDYFPPNITVKVNSKLCPLPNPIPTNKPGVEPKRPPRPVNISPLVKLSPTVGNEIRVSWSADYGRRFAVAVYLVRKLTSAELLSRLKNRGARHSDYTRGLIKEKLNEDADSEIATTSLRVSLACPLGKMRMCTPCRASTCSHLQCFDASLFLQMNERKPTWNCPVCDKSALYDNLVIDGYFQEVLNSNKLLPDVNEIQLLQDGSWENL 498          

BLAST of EMLSAG00000000339 vs. nr
Match: gi|951579583|ref|XP_014487437.1| (PREDICTED: E3 SUMO-protein ligase PIAS2 isoform X1 [Dinoponera quadriceps])

HSP 1 Score: 367.851 bits (943), Expect = 4.169e-115
Identity = 218/543 (40.15%), Postives = 302/543 (55.62%), Query Frame = 0

Query:    7 VQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIRELNNEMPRPGIGIGNNNKESPLNQLLGERSTRSRKGESDNPSSNALEEAPVTRRGARSTRTRVSYAEP----DEDETSEVLSDSSXDESEESD-----KGYNPPSEEEEVVESGGEDTDSAIPLSPSHSPTRLHSKKSANLPNILKKSPRGHISNTGSKTIISSLLPTIKTADILVPHTKTVVPIQNSEPKYDIANNGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEAL 538
            ++ M+++FR+SELQ L+ +AG++KSGRK               N+++ +  EL +    P I +        + +L     T     ++D  +         T    RS+ +   Y +P    D+    ++ S +      + D     + Y    +     +       +   L+P+H          A++P   + +P     +TG   + +   P+   A    P+   V+P         +       YP  PDV+L  LPF+ +   LLKPSSL P  S R Q  TF+FHLTP QA  IA S    A  K++Y  Q+QMRFCL ETSC Q+D FP +I V VN +   LPN IPTNKPGVEPKRPP+P+NI+   KLS T+ N + V+W+ + G+ F VA+Y V KLTS  L+  LK++G RHS YT+ +IK+KLN D D EIATTS +V+LACPLGKMRM  PCRA+TC HLQCFDA L++QMNERKP W CPVC+K+A+Y+NL++DGYF EVLN  KL  + NEI L  D  WE L
Sbjct:   11 LRNMILSFRVSELQMLLGFAGRNKSGRK---------------NELQARALELLHLRSHPAIRLK-------IRELYKTIHTSYSIHDADRTAFIYKSRYSGTSLYVRSSNSSCRYDKPYVRQDQMAAHQIYSQTGSSTEPQIDQTMHNRNYYSTRQAMSQQQQSQASVSAGKELTPAHQ---------ASIPQAPRTNPV--YQSTGYTNVTAQRAPSAGYA--YSPYPPKVLP-------SPLQMQPATQYPVHPDVRLKKLPFFDLLGELLKPSSLMPQGSLRLQENTFMFHLTPQQATDIASSRDCRAGSKMDYIVQVQMRFCLQETSCEQEDYFPPNITVKVNSKLCPLPNPIPTNKPGVEPKRPPRPVNISPLVKLSPTVGNEIRVSWSADYGRRFAVAVYLVRKLTSAELLSRLKNRGARHSDYTRGLIKEKLNEDADSEIATTSLRVSLACPLGKMRMCTPCRASTCSHLQCFDASLFLQMNERKPTWNCPVCDKSALYDNLVIDGYFQEVLNSNKLLPDVNEIQLLQDGSWENL 511          

BLAST of EMLSAG00000000339 vs. nr
Match: gi|571527013|ref|XP_006561119.1| (PREDICTED: E3 SUMO-protein ligase PIAS3 isoform X3 [Apis mellifera] >gi|572271434|ref|XP_006613661.1| PREDICTED: E3 SUMO-protein ligase PIAS3-like isoform X3 [Apis dorsata] >gi|1035605312|ref|XP_016910162.1| PREDICTED: E3 SUMO-protein ligase PIAS3 isoform X3 [Apis cerana])

HSP 1 Score: 361.303 bits (926), Expect = 4.101e-114
Identity = 176/291 (60.48%), Postives = 213/291 (73.20%), Query Frame = 0

Query:  250 YPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEAL 538
            YP  PDV+L  LPF+ + A LLKPSSL P  S R Q  TF+FHLTP Q+  +A S    A  K++Y  QIQMRFCL ETSC Q+D FP SI V VNG+   LPN IPTNKPGVEPKRPP+P+NI+   KLS T+ N ++VTW+ + G+ + +AIY V KL+S  L+  LK +G RHS YT+ +IK+KLN D D EIATTS +V+LACPLGKMRMS PCRATTC HLQCFDA L++QMNERKP W CPVC+K A+Y+NL++DGYF EVLN  KL  + NEI L  D  WE L
Sbjct:  101 YPVHPDVRLKKLPFFDLLAELLKPSSLMPQGSMRLQENTFMFHLTPQQSTDVASSRDCRAGSKMDYTVQIQMRFCLQETSCEQEDCFPPSIAVKVNGKLCPLPNPIPTNKPGVEPKRPPRPVNISPLVKLSPTVANQIHVTWSADYGRRYAIAIYLVRKLSSAELLSRLKSRGVRHSDYTRGLIKEKLNEDADSEIATTSLRVSLACPLGKMRMSTPCRATTCSHLQCFDASLFLQMNERKPTWNCPVCDKPALYDNLVIDGYFQEVLNSKKLLPDVNEIQLLQDGSWENL 391          

BLAST of EMLSAG00000000339 vs. nr
Match: gi|328780413|ref|XP_623571.3| (PREDICTED: E3 SUMO-protein ligase PIAS3 isoform X1 [Apis mellifera] >gi|572271430|ref|XP_006613659.1| PREDICTED: E3 SUMO-protein ligase PIAS3-like isoform X1 [Apis dorsata] >gi|1035605308|ref|XP_016910160.1| PREDICTED: E3 SUMO-protein ligase PIAS3 isoform X1 [Apis cerana])

HSP 1 Score: 362.459 bits (929), Expect = 1.148e-113
Identity = 176/291 (60.48%), Postives = 213/291 (73.20%), Query Frame = 0

Query:  250 YPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEAL 538
            YP  PDV+L  LPF+ + A LLKPSSL P  S R Q  TF+FHLTP Q+  +A S    A  K++Y  QIQMRFCL ETSC Q+D FP SI V VNG+   LPN IPTNKPGVEPKRPP+P+NI+   KLS T+ N ++VTW+ + G+ + +AIY V KL+S  L+  LK +G RHS YT+ +IK+KLN D D EIATTS +V+LACPLGKMRMS PCRATTC HLQCFDA L++QMNERKP W CPVC+K A+Y+NL++DGYF EVLN  KL  + NEI L  D  WE L
Sbjct:  173 YPVHPDVRLKKLPFFDLLAELLKPSSLMPQGSMRLQENTFMFHLTPQQSTDVASSRDCRAGSKMDYTVQIQMRFCLQETSCEQEDCFPPSIAVKVNGKLCPLPNPIPTNKPGVEPKRPPRPVNISPLVKLSPTVANQIHVTWSADYGRRYAIAIYLVRKLSSAELLSRLKSRGVRHSDYTRGLIKEKLNEDADSEIATTSLRVSLACPLGKMRMSTPCRATTCSHLQCFDASLFLQMNERKPTWNCPVCDKPALYDNLVIDGYFQEVLNSKKLLPDVNEIQLLQDGSWENL 463          

HSP 2 Score: 73.1738 bits (178), Expect = 6.876e-10
Identity = 32/59 (54.24%), Postives = 50/59 (84.75%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIREL 59
            MA+ + ++ M+++FR+SELQ L+ +AG++KSGRK++LQ RAL L+RL S+ I++KIREL
Sbjct:    1 MAETKELENMILSFRVSELQMLLGFAGRNKSGRKNELQGRALELLRLRSHPIQLKIREL 59          

BLAST of EMLSAG00000000339 vs. nr
Match: gi|970883465|ref|XP_015125936.1| (PREDICTED: E3 SUMO-protein ligase PIAS3 [Diachasma alloeum])

HSP 1 Score: 362.844 bits (930), Expect = 1.182e-113
Identity = 178/293 (60.75%), Postives = 215/293 (73.38%), Query Frame = 0

Query:  250 YPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDAT--GKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEAL 538
            YP  PDV+L  LPFY +   LLKPSSL P  S R Q  +F FHLTP QA+ IA   YRD     K+EY  Q+QMRFCL ETSC Q+D FP S+ V VNG+   LPN IPTNKPGVEPKRPP+P+NI+   KLS T+ N + VTW+ + G+ + VAIY V+KLTS+ L+  LK++G RHS YT+ +IK+KLN D D EIATTS +V+LACPLGKMRMS PCRA+TC HLQCFDA LY+QMNERKP W CPVC+K A+Y+NL++DGYF EVL   KL ++ NEI L  D  WE L
Sbjct:  176 YPVHPDVRLKKLPFYDLLGELLKPSSLMPQGSMRLQENSFQFHLTPQQASDIAT--YRDCRPGSKMEYIIQVQMRFCLQETSCEQEDYFPPSVQVKVNGKQCPLPNPIPTNKPGVEPKRPPRPVNISPLVKLSPTVANTIQVTWSADYGRRYAVAIYMVKKLTSSELLSRLKNRGARHSDYTRGLIKEKLNEDADSEIATTSLRVSLACPLGKMRMSTPCRASTCLHLQCFDASLYLQMNERKPTWNCPVCDKTALYDNLVIDGYFQEVLLSKKLLSDVNEIQLLQDGSWENL 466          

BLAST of EMLSAG00000000339 vs. nr
Match: gi|571527009|ref|XP_006561118.1| (PREDICTED: E3 SUMO-protein ligase PIAS3 isoform X2 [Apis mellifera] >gi|572271432|ref|XP_006613660.1| PREDICTED: E3 SUMO-protein ligase PIAS3-like isoform X2 [Apis dorsata] >gi|1035605310|ref|XP_016910161.1| PREDICTED: E3 SUMO-protein ligase PIAS3 isoform X2 [Apis cerana])

HSP 1 Score: 362.073 bits (928), Expect = 1.321e-113
Identity = 176/291 (60.48%), Postives = 213/291 (73.20%), Query Frame = 0

Query:  250 YPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWEAL 538
            YP  PDV+L  LPF+ + A LLKPSSL P  S R Q  TF+FHLTP Q+  +A S    A  K++Y  QIQMRFCL ETSC Q+D FP SI V VNG+   LPN IPTNKPGVEPKRPP+P+NI+   KLS T+ N ++VTW+ + G+ + +AIY V KL+S  L+  LK +G RHS YT+ +IK+KLN D D EIATTS +V+LACPLGKMRMS PCRATTC HLQCFDA L++QMNERKP W CPVC+K A+Y+NL++DGYF EVLN  KL  + NEI L  D  WE L
Sbjct:  164 YPVHPDVRLKKLPFFDLLAELLKPSSLMPQGSMRLQENTFMFHLTPQQSTDVASSRDCRAGSKMDYTVQIQMRFCLQETSCEQEDCFPPSIAVKVNGKLCPLPNPIPTNKPGVEPKRPPRPVNISPLVKLSPTVANQIHVTWSADYGRRYAIAIYLVRKLSSAELLSRLKSRGVRHSDYTRGLIKEKLNEDADSEIATTSLRVSLACPLGKMRMSTPCRATTCSHLQCFDASLFLQMNERKPTWNCPVCDKPALYDNLVIDGYFQEVLNSKKLLPDVNEIQLLQDGSWENL 454          

HSP 2 Score: 70.0922 bits (170), Expect = 6.481e-9
Identity = 30/50 (60.00%), Postives = 44/50 (88.00%), Query Frame = 0

Query:   10 MVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIREL 59
            M+++FR+SELQ L+ +AG++KSGRK++LQ RAL L+RL S+ I++KIREL
Sbjct:    1 MILSFRVSELQMLLGFAGRNKSGRKNELQGRALELLRLRSHPIQLKIREL 50          

BLAST of EMLSAG00000000339 vs. nr
Match: gi|380025134|ref|XP_003696334.1| (PREDICTED: LOW QUALITY PROTEIN: E3 SUMO-protein ligase PIAS3 [Apis florea])

HSP 1 Score: 361.688 bits (927), Expect = 2.181e-113
Identity = 175/289 (60.55%), Postives = 212/289 (73.36%), Query Frame = 0

Query:  250 YPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLN-DRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTE-NEIVLKNDAEWE 536
            YP  PDV+L  LPF+ + A LLKPSSL P  S R Q  TF+FHLTP Q+  +A S    A  K++Y  QIQMRFCL ETSC Q+D FP SI V VNG+   LPN IPTNKPGVEPKRPP+P+NI+   KLS T+ N ++VTW+ + G+ + +AIY V KL+S  L+  LK +G RHS YT+ +IK+KLN D D EIATTS +V+LACPLGKMRMS PCRATTC HLQCFDA L++QMNERKP W CPVC+K A+Y+NL++DGYF EVLN  KL  + NEI L  D  WE
Sbjct:  173 YPVHPDVRLKKLPFFDLLAELLKPSSLMPQGSMRLQENTFMFHLTPQQSTDVASSRDCRAGSKMDYTVQIQMRFCLQETSCEQEDCFPPSIAVKVNGKLCPLPNPIPTNKPGVEPKRPPRPVNISPLVKLSPTVANQIHVTWSADYGRRYAIAIYLVRKLSSAELLSRLKSRGVRHSDYTRGLIKEKLNEDADSEIATTSLRVSLACPLGKMRMSTPCRATTCSHLQCFDASLFLQMNERKPTWNCPVCDKPALYDNLVIDGYFQEVLNSKKLLPDVNEIQLLQDGSWE 461          

HSP 2 Score: 73.1738 bits (178), Expect = 6.248e-10
Identity = 32/59 (54.24%), Postives = 50/59 (84.75%), Query Frame = 0

Query:    1 MADLEMVQAMVMNFRLSELQALMAYAGKSKSGRKSDLQIRALNLIRLNSNDIKMKIREL 59
            MA+ + ++ M+++FR+SELQ L+ +AG++KSGRK++LQ RAL L+RL S+ I++KIREL
Sbjct:    1 MAETKELENMILSFRVSELQMLLGFAGRNKSGRKNELQGRALELLRLRSHPIQLKIREL 59          

BLAST of EMLSAG00000000339 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold316_size209483-snap-gene-1.37 (protein:Tk05945 transcript:maker-scaffold316_size209483-snap-gene-1.37-mRNA-1 annotation:"e3 sumo-protein ligase pias2 isoform x6")

HSP 1 Score: 463.766 bits (1192), Expect = 5.538e-155
Identity = 224/395 (56.71%), Postives = 281/395 (71.14%), Query Frame = 0

Query:  245 NGHLMYPPCPDVQLVSLPFYKVEAILLKPSSLNPSDSARFQVQTFVFHLTPSQANAIAGSGYRDATGKLEYKNQIQMRFCLNETSCPQDDNFPASICVIVNGRSQALPNIIPTNKPGVEPKRPPKPINITSNCKLSATLPNYLNVTWTVEAGKGFCVAIYYVEKLTSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIATTSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKL-GTENEIVLKNDAEWEALPSKDEESSSSSQKPKSIQNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATT--------TNKASEDK-IVECITLD-DSDEESPANPPAKKTR 628
            N HLMYP  PDV+L  LPF+KV A L++P +L P  +ARFQ Q   FHL+PSQA+ IA S +RD+ G++EYK QIQMRF L ETSC QDDNFPASICV VN + Q LPN IPTNKPGVEPKRPPKPINIT+ CKLS+T+ NYL+V+W VE G+GF V+IYYV+KL+S  L+K LK++G RH  YT+A+IK+KLND+D ++ TTSCKVTLACPLGKMRMS PCRA+TCDHLQCFDA L++ MNERKPKW+CPVCNK A+++NL +DG+F EVL   KL   ++E+VL ND  WE LP K E  SSS ++P          P  +  +E V+  + EDDE        ++ +  +        + K   DK   +CITLD DSD+E P   P  + R
Sbjct:  285 NSHLMYPTFPDVKLRQLPFFKVMATLMQPCALQPGSTARFQEQKLSFHLSPSQASEIASSSFRDSLGRVEYKKQIQMRFSLLETSCEQDDNFPASICVKVNNKMQPLPNPIPTNKPGVEPKRPPKPINITALCKLSSTMANYLDVSWAVEIGRGFTVSIYYVDKLSSLDLMKDLKERGIRHPDYTRAVIKEKLNDKDVDVMTTSCKVTLACPLGKMRMSYPCRASTCDHLQCFDANLFLMMNERKPKWLCPVCNKGALFDNLQIDGFFNEVLKSAKLPAHDHEVVLHNDGSWEPLPYKKEGVSSSDREPT---------PPPSAKIEKVETLNVEDDEVPIPIPPPAVNNGHSDLGSLPGVSPKTKNDKRSFDCITLDSDSDDEGPETRPPNRKR 670          

BLAST of EMLSAG00000000339 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold285_size222332-snap-gene-1.20 (protein:Tk07155 transcript:maker-scaffold285_size222332-snap-gene-1.20-mRNA-1 annotation:"zinc finger miz domain-containing protein 1")

HSP 1 Score: 92.8189 bits (229), Expect = 1.190e-19
Identity = 71/259 (27.41%), Postives = 118/259 (45.56%), Query Frame = 0

Query:  388 NVTWTVEAGKGFCVAIYYVEKL----TSNTLIKILKDKGNRHSAYTKAIIKDKLNDRDCEIAT---------------TSCKVTLACPLGKMRMSLPCRATTCDHLQCFDARLYIQMNERKPKWICPVCNKNAMYENLMVDGYFFEVLNDPKLGTENEIVLKNDAEWEAL-PSKDEESSSSSQK---------PKSIQNPIIAPPVNNTSVETVDVYDDEDDEAVKAAKAASLKSATTTNKASE--DKIVECITLDDSD 615
            N+ +TV A    C +  +V +L    T  ++++ L  K  R       + K K    +C  A+               TS KV L CPL   RM+LP R   C HLQCFD   Y++ N  K  W CPVCNKN   E L +D + + +L  PK    +E+++  +A    + P K+E   S  +K         P +++ P +A     +S+ +  +Y   D  ++ + +    +S    ++ +E  +KI +   +DD +
Sbjct:  525 NIQFTVTA---CCCSHLFVVQLVHRPTVRSIVQGLLRK--RLLPMEHGVTKSKRILTNCNNASISKDPGSNLSDPSDATSLKVMLTCPLTYKRMTLPARGQECRHLQCFDLESYLKYNGDKGSWKCPVCNKNTPLEGLEIDQFTWSILQTPKFSEVDEVMIDQNAGIALIHPFKNEIDPSGGRKGSPKDHPMAPNALKLPNLALWEIPSSIRSPGLYTPPDINSIVSGQYPLEESGGPLSQMNEAVNKIDQPRNMDDPN 778