EMLSAG00000000488, EMLSAG00000000488-683254 (gene) Lepeophtheirus salmonis

Overview
NameEMLSAG00000000488
Unique NameEMLSAG00000000488-683254
Typegene
OrganismLepeophtheirus salmonis (salmon louse)
Associated RNAi Experiments

Nothing found

Homology
BLAST of EMLSAG00000000488 vs. GO
Match: - (symbol:CG10254 species:7227 "Drosophila melanogaster" [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISS] InterPro:IPR000608 Pfam:PF00179 PROSITE:PS50127 EMBL:AE014297 GO:GO:0016567 GO:GO:0004842 Gene3D:3.10.110.10 InterPro:IPR016135 SUPFAM:SSF54495 eggNOG:COG5078 GeneTree:ENSGT00730000110680 KO:K10581 OrthoDB:EOG7GN2KX EMBL:AF132190 RefSeq:NP_651170.1 RefSeq:NP_732888.1 UniGene:Dm.3186 SMR:Q9XZ32 MINT:MINT-894013 STRING:7227.FBpp0083867 EnsemblMetazoa:FBtr0084476 EnsemblMetazoa:FBtr0084477 GeneID:42793 KEGG:dme:Dmel_CG10254 UCSC:CG10254-RA FlyBase:FBgn0027512 InParanoid:Q9XZ32 OMA:DRTLMPG GenomeRNAi:42793 NextBio:830588 PRO:PR:Q9XZ32 Uniprot:Q9XZ32)

HSP 1 Score: 294.278 bits (752), Expect = 5.455e-81
Identity = 132/268 (49.25%), Postives = 182/268 (67.91%), Query Frame = 0
Query:  848 FKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZIS-SSYHSNKKEISPL-----------DFPLLPASKGFCVSFAKSLDEF 1103
            F++  +AP  H F  +L  P +   + + V++E  +L SSLP+G+V++ YEDR+DL S M+ GP  TPY + +FFFDFQ   DYPKSPP C YIS+C+DRLNPNLYE G+VCVSLLGTW G+  E+W+P+S +LQ+L+SIQGLILV EPY+NEAGYE Q+ T+ G ENS +YNEM +IKI QS  + L +PP IF++E++EH  E G +   R R W + S  +   N  +I  +           +FPLLP S+GFC++  + L + 
Sbjct: 1097 FQVLPNAPAAHNFISNLITPANKAQYQRAVQREYLMLKSSLPNGVVVRAYEDRMDLMSVMMVGPKRTPYQNALFFFDFQFGRDYPKSPPVCHYISYCTDRLNPNLYEGGRVCVSLLGTWMGRDNEVWSPSSTMLQVLVSIQGLILVDEPYYNEAGYEKQRGTQLGNENSRVYNEMAIIKIAQSTVKQLTNPPLIFRNELIEHFKEFGTELYARMRAWSEYSLEAQRQNITKIKDMPVEYKASCELPEFPLLPCSRGFCIAVKRVLSQL 1364          

HSP 2 Score: 218.779 bits (556), Expect = 1.230e-56
Identity = 124/319 (38.87%), Postives = 187/319 (58.62%), Query Frame = 0
Query:  370 RRRLKKRNKSVTAELYSNLPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLG-GLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANII 687
            RR++KK+ +SV   +YS  P       PK   G+ +VVE L   + V+V+WQDG++E G+ S++LYPIHHLD+ EFF G+FV +A+D+  G   YGV+Q VD+ +RIAK+ WF+ Y    +P P + G  E SVYD++DH D+ YRPG+VVI+V+NF  E+      G+VI+ YPNG+V VWWA    T C+PQDL++  E D    E  + D D  G +     EDSW          E+E  +  I S      I+ G+ ++   +++ LE+ F      + + +L  L ++YK+C  L++ + T +F    F  I+
Sbjct:  514 RRQVKKKIRSVKKTIYS--PFNKKETTPK--DGDEIVVETLVVYSSVTVVWQDGTVESGVHSTQLYPIHHLDNHEFFPGDFVTKANDISTGATDYGVIQCVDHDARIAKVMWFNIYTNIDNPIPLFKGLEELSVYDMKDHTDYQYRPGTVVIRVSNFTGEDV-NSTAGQVIDNYPNGRVRVWWAKGHITLCYPQDLFQPFEQD----EQNEQDQDVYGSDG---SEDSW----------ETESENSHISSSDNDDDILTGI-DRATEAMKVLEKMFRVLHPNRKAEVLYDLLDVYKNCRALDRLLNTKFFDEFNFKGIV 809          

HSP 3 Score: 127.102 bits (318), Expect = 4.076e-28
Identity = 97/326 (29.75%), Postives = 156/326 (47.85%), Query Frame = 0
Query:   10 AQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRM----DSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVK-----VTVLKTQVRELSVNWQCQACVGNE--SDGIAPD-------IIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQRERE 317
            +Q F ED V+ +D++G + FG V    E   +  SE+ED D   L  GEV V++YP  +E V    S+ L +R+LMPGDVVRR     + L    Q GY R++ VR    +  S   VI ++ +E L+PI  +     +C+  W+G I  +     L   +G +R  +  N    F D   +  + T+F    ++PG I+ G +            D++K L  +      +      TV   +   ++V+W C+A +GN   SD    D       +I+GE+L + K L  +    LQI DRF+ K  + D+++  + W   + E
Sbjct:   80 SQFFLEDGVFRIDRRGRVHFGLVTATTE--TYSSSEEEDVD--SLSRGEVRVAFYPGGKEMVMTEQSIGLADRTLMPGDVVRRRLPGENVLA--GQAGYVRDVNVRADVMVMGSR-MVIKNVPAERLRPISEWNQYVPLCMDTWIGTIIDINECAVLKSSNG-ARVEITSNDFHKFKDAVSECDR-TVFNPNVFFPGNIVIGRM---------PPPDRVKNLTPEITHPLTRKGRAVYTVESVETTSINVDWSCRA-IGNATGSDDTDSDPLKEPRSLIKGEELHKVKRLSLYESYELQIHDRFYLKYSKCDLLVKYADWENEQAE 386          
BLAST of EMLSAG00000000488 vs. GO
Match: - (symbol:UBE2O "Ubiquitin-conjugating enzyme E2 O" species:9606 "Homo sapiens" [GO:0016881 "acid-amino acid ligase activity" evidence=IEA] InterPro:IPR000608 Pfam:PF00179 PROSITE:PS50127 GO:GO:0008152 GO:GO:0016881 Gene3D:3.10.110.10 InterPro:IPR016135 SUPFAM:SSF54495 EMBL:AC090699 EMBL:AC015802 HGNC:HGNC:29554 ProteinModelPortal:K7ES11 Ensembl:ENST00000587127 Uniprot:K7ES11)

HSP 1 Score: 267.314 bits (682), Expect = 4.008e-74
Identity = 125/226 (55.31%), Postives = 162/226 (71.68%), Query Frame = 0
Query:  844 KNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISS 1069
            K   F + E AP  H+FK   F+P + K F   V+KE  LL++SLP GI++K +EDR+DLFSA+IKGP+ TPY DG++ FD QLP  YP  PP   Y+S CS RLNPNLY+ GKVCVSLLGTW GKGTE WT  S+LLQ+L+SIQGLILV EPY+NEAG++S +  + G ENS  YNEM +I++VQSMT+L+  PP +F+ EI +H +  G + V R   WL+  +
Sbjct:  440 KGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHA 665          

HSP 2 Score: 132.88 bits (333), Expect = 3.591e-30
Identity = 81/204 (39.71%), Postives = 110/204 (53.92%), Query Frame = 0
Query:  400 RPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVV--RASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEE-SVYDLRDHPDFNYRPGSVVIKVANFDN----EESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKV------GEYDSDEGEL-------WDDDSD 583
            +PG+ V VE + T     V+WQDGS+E  I S++L+P+HHLD+ EF  G+FVV  R         YGVVQ  D+  R   +KWF     G D     +GE E+ SVYD+ DHPDF +R   +VI++ N ++    +E E    G+V  V  + KV V WA+   T   PQ LY +       +YDS EG         W+DDSD
Sbjct:   53 KPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDD--VELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEQPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSD 254          
BLAST of EMLSAG00000000488 vs. GO
Match: - (symbol:Ube2o "ubiquitin-conjugating enzyme E2O" species:10090 "Mus musculus" [GO:0000166 "nucleotide binding" evidence=IEA] [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0005524 "ATP binding" evidence=IEA] [GO:0005575 "cellular_component" evidence=ND] [GO:0008150 "biological_process" evidence=ND] [GO:0016874 "ligase activity" evidence=IEA] [GO:0016881 "acid-amino acid ligase activity" evidence=IEA] [GO:0044822 "poly(A) RNA binding" evidence=ISO] InterPro:IPR000608 Pfam:PF00179 PROSITE:PS50127 UniPathway:UPA00143 MGI:MGI:2444266 GO:GO:0005524 GO:GO:0004842 Gene3D:3.10.110.10 InterPro:IPR016135 SUPFAM:SSF54495 eggNOG:COG5078 GeneTree:ENSGT00730000110680 EMBL:AL607039 EMBL:AL645851 KO:K10581 CTD:63893 HOVERGEN:HBG080116 OMA:DLQHIWP OrthoDB:EOG7GN2KX TreeFam:TF325556 ChiTaRS:UBE2O EMBL:AK129431 EMBL:U20780 EMBL:BC059193 EMBL:AK045398 PIR:I49264 RefSeq:NP_776116.2 UniGene:Mm.243950 ProteinModelPortal:Q6ZPJ3 SMR:Q6ZPJ3 BioGrid:229895 IntAct:Q6ZPJ3 MINT:MINT-7034165 PhosphoSite:Q6ZPJ3 PaxDb:Q6ZPJ3 PRIDE:Q6ZPJ3 Ensembl:ENSMUST00000082152 GeneID:217342 KEGG:mmu:217342 UCSC:uc007mlm.1 HOGENOM:HOG000231096 InParanoid:A2A7X3 NextBio:375789 PRO:PR:Q6ZPJ3 Bgee:Q6ZPJ3 CleanEx:MM_UBE2O Genevestigator:Q6ZPJ3 Uniprot:Q6ZPJ3)

HSP 1 Score: 268.855 bits (686), Expect = 6.360e-73
Identity = 134/265 (50.57%), Postives = 177/265 (66.79%), Query Frame = 0
Query:  807 EHKIEAPQVEKVVVS--PPEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISS 1069
            E K E P+V+  V +  P E  +   +    P     + K   F + E AP  H+FK   F+P + K F   V+KE  LL++SLP GI++K +EDR+DLFSA+IKGP+ TPY DG++ FD QLP  YP  PP   Y+S CS RLNPNLY+ GKVCVSLLGTW GKGTE WT  S+LLQ+L+SIQGLILV EPY+NEAG++S +  + G ENS  YNEM +I++VQSMT+L+  PP +F+ EI +H +  G + V R   WL+  +
Sbjct:  883 ERKEEKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLVNRIESWLETHA 1147          

HSP 2 Score: 130.954 bits (328), Expect = 2.190e-29
Identity = 81/205 (39.51%), Postives = 112/205 (54.63%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVV--RASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEE-SVYDLRDHPDFNYRPGSVVIKVANFDN----EESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKV------GEYDSDEG-------ELWDDDSD 583
             +PG+ V VE + T     V+WQDGS+E  I S++L+P+HHLD+ EF  G+FVV  R         YGVVQ  D+  R   +KWF     G D     +GE E+ SVYD+ DHPDF +R   +VI++ N ++    +E E  + G+V  V  + KV V WA+   T   PQ LY +       +YDS EG       + W+DDSD
Sbjct:  533 FKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDD--VELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSV-GQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSSSGASSDEWEDDSD 734          

HSP 3 Score: 98.9821 bits (245), Expect = 1.688e-19
Identity = 79/248 (31.85%), Postives = 125/248 (50.40%), Query Frame = 0
Query:   54 LKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLF--KRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIA--PDIIQGEDLKRTKILGYFFPCTLQIGDRFFY 297
            L+ G V V WYP+  ++    + LKL +RS++P DVVR M S    SQ G   ++ +  +  +  +N  +IY +NS+DL+ I  F+    +    W+G +  +K  + L   +G +RC ++    +   DV        LF      +YPG +L GP + F   +W S    +K ++S K  KF +V V + QV EL V W  ++     +D ++  P II  E+L R K LG F     Q+G+R  Y
Sbjct:  112 LRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRST--DSQCGTVIDVNIDCAVKLIGTN-CIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNG-ARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLS---GVKPVLSTK-SKF-RVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSIITQENLGRVKRLGCFDHAQRQLGERCLY 350          
BLAST of EMLSAG00000000488 vs. GO
Match: - (symbol:Ube2o "Protein Ube2o" species:10116 "Rattus norvegicus" [GO:0016881 "acid-amino acid ligase activity" evidence=IEA] InterPro:IPR000608 Pfam:PF00179 PROSITE:PS50127 RGD:1310297 GO:GO:0008152 GO:GO:0016881 Gene3D:3.10.110.10 InterPro:IPR016135 SUPFAM:SSF54495 GeneTree:ENSGT00730000110680 OMA:DLQHIWP OrthoDB:EOG7GN2KX EMBL:AABR06066252 EMBL:AABR06066253 PRIDE:F1M403 Ensembl:ENSRNOT00000014699 NextBio:35581055 Uniprot:F1M403)

HSP 1 Score: 268.085 bits (684), Expect = 1.330e-72
Identity = 133/262 (50.76%), Postives = 176/262 (67.18%), Query Frame = 0
Query:  807 EHKIEAPQVEKVVVS--PPEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZ 1066
            E K + P+V+  V +  P E  +   +    P     + K   F + E AP  H+FK   F+P + K F   V+KE  LL++SLP GI++K +EDR+DLFSA+IKGP+ TPY DG++ FD QLP  YP  PP   Y+S CS RLNPNLY+ GKVCVSLLGTW GKGTE WT  S+LLQ+L+SIQGLILV EPY+NEAG++S +  + G ENS  YNEM +I++VQSMT+L+  PP +F+ EI +H +  G + V R   WL+
Sbjct:  882 ERKEDKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLVNRIESWLE 1143          

HSP 2 Score: 130.954 bits (328), Expect = 2.574e-29
Identity = 81/205 (39.51%), Postives = 111/205 (54.15%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVV--RASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEE-SVYDLRDHPDFNYRPGSVVIKVANFDN----EESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKV------GEYDSDEGEL-------WDDDSD 583
             +PG+ V VE + T     V+WQDGS+E  I S++L+P+HHLD+ EF  G+FVV  R         YGVVQ  D+  R   +KWF     G D     +GE E+ SVYD+ DHPDF +R   +VI++ N ++    +E E  + G+V  V  + KV V WA+   T   PQ LY +       +YDS EG         W+DDSD
Sbjct:  532 FKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDD--VELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSV-GQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSD 733          

HSP 3 Score: 98.5969 bits (244), Expect = 2.052e-19
Identity = 78/248 (31.45%), Postives = 125/248 (50.40%), Query Frame = 0
Query:   54 LKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLF--KRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIA--PDIIQGEDLKRTKILGYFFPCTLQIGDRFFY 297
            L+ G V V WYP+  ++    + LKL +RS++P DVVR M S    SQ G   ++ +  +  +  +N  +IY +NS+DL+ I  F+    +    W+G +  +K  + L   +G +RC ++    +   DV        LF      +YPG +L GP + F   +W S    +K ++S K  KF +V V + QV EL V W  ++     +D ++  P +I  E+L R K LG F     Q+G+R  Y
Sbjct:  111 LRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRST--DSQCGTVIDVNIDCAVKLIGTN-CIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNG-ARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLS---GVKPVLSTK-SKF-RVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVIAQENLGRVKRLGCFDHAQRQLGERCLY 349          
BLAST of EMLSAG00000000488 vs. GO
Match: - (symbol:Ube2o "ubiquitin-conjugating enzyme E2O" species:10116 "Rattus norvegicus" [GO:0016881 "acid-amino acid ligase activity" evidence=IEA] [GO:0044822 "poly(A) RNA binding" evidence=ISO] InterPro:IPR000608 Pfam:PF00179 PROSITE:PS50127 RGD:1310297 GO:GO:0008152 GO:GO:0016881 Gene3D:3.10.110.10 InterPro:IPR016135 SUPFAM:SSF54495 GeneTree:ENSGT00730000110680 OMA:DLQHIWP OrthoDB:EOG7GN2KX EMBL:AABR06066252 EMBL:AABR06066253 PRIDE:F1M403 Ensembl:ENSRNOT00000014699 NextBio:35581055 Uniprot:F1M403)

HSP 1 Score: 268.085 bits (684), Expect = 1.330e-72
Identity = 133/262 (50.76%), Postives = 176/262 (67.18%), Query Frame = 0
Query:  807 EHKIEAPQVEKVVVS--PPEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZ 1066
            E K + P+V+  V +  P E  +   +    P     + K   F + E AP  H+FK   F+P + K F   V+KE  LL++SLP GI++K +EDR+DLFSA+IKGP+ TPY DG++ FD QLP  YP  PP   Y+S CS RLNPNLY+ GKVCVSLLGTW GKGTE WT  S+LLQ+L+SIQGLILV EPY+NEAG++S +  + G ENS  YNEM +I++VQSMT+L+  PP +F+ EI +H +  G + V R   WL+
Sbjct:  882 ERKEDKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLVNRIESWLE 1143          

HSP 2 Score: 130.954 bits (328), Expect = 2.574e-29
Identity = 81/205 (39.51%), Postives = 111/205 (54.15%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVV--RASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEE-SVYDLRDHPDFNYRPGSVVIKVANFDN----EESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKV------GEYDSDEGEL-------WDDDSD 583
             +PG+ V VE + T     V+WQDGS+E  I S++L+P+HHLD+ EF  G+FVV  R         YGVVQ  D+  R   +KWF     G D     +GE E+ SVYD+ DHPDF +R   +VI++ N ++    +E E  + G+V  V  + KV V WA+   T   PQ LY +       +YDS EG         W+DDSD
Sbjct:  532 FKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDD--VELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSV-GQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSD 733          

HSP 3 Score: 98.5969 bits (244), Expect = 2.052e-19
Identity = 78/248 (31.45%), Postives = 125/248 (50.40%), Query Frame = 0
Query:   54 LKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLF--KRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIA--PDIIQGEDLKRTKILGYFFPCTLQIGDRFFY 297
            L+ G V V WYP+  ++    + LKL +RS++P DVVR M S    SQ G   ++ +  +  +  +N  +IY +NS+DL+ I  F+    +    W+G +  +K  + L   +G +RC ++    +   DV        LF      +YPG +L GP + F   +W S    +K ++S K  KF +V V + QV EL V W  ++     +D ++  P +I  E+L R K LG F     Q+G+R  Y
Sbjct:  111 LRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRST--DSQCGTVIDVNIDCAVKLIGTN-CIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNG-ARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLS---GVKPVLSTK-SKF-RVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVIAQENLGRVKRLGCFDHAQRQLGERCLY 349          
BLAST of EMLSAG00000000488 vs. GO
Match: - (symbol:UBE2O "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0016881 "acid-amino acid ligase activity" evidence=IEA] InterPro:IPR000608 Pfam:PF00179 PROSITE:PS50127 GO:GO:0008152 GO:GO:0016881 Gene3D:3.10.110.10 InterPro:IPR016135 SUPFAM:SSF54495 GeneTree:ENSGT00730000110680 OMA:DLQHIWP OrthoDB:EOG7GN2KX TreeFam:TF325556 EMBL:AAEX03006281 Ensembl:ENSCAFT00000008179 Uniprot:F1PG13)

HSP 1 Score: 266.544 bits (680), Expect = 1.444e-72
Identity = 125/226 (55.31%), Postives = 161/226 (71.24%), Query Frame = 0
Query:  844 KNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISS 1069
            K   F + E AP  H+FK   F+P + K F   V+KE  LL++SLP GI++K +EDR+DLFSA+IKGP+ TPY DG++ FD QLP  YP  PP   Y+S CS RLNPNLY+ GKVCVSLLGTW GKGTE WT  S+LLQ+L+SIQGLILV EPY+NEAG++S +  + G ENS  YNEM +I++VQSMT+L+  PP +F+ EI +H    G + V R   WL+  +
Sbjct:  771 KGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFRTGGWRLVSRIESWLETHA 996          

HSP 2 Score: 131.724 bits (330), Expect = 1.392e-29
Identity = 81/205 (39.51%), Postives = 111/205 (54.15%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVV--RASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEE-SVYDLRDHPDFNYRPGSVVIKVANFDN----EESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKV------GEYDSDEGEL-------WDDDSD 583
             +PG+ V VE + T     V+WQDGS+E  I S++L+P+HHLD+ EF  G+FVV  R         YGVVQ  D+  R   +KWF     G D     +GE E+ SVYD+ DHPDF +R   +VI++ N ++    +E E  + G+V  V  + KV V WA+   T   PQ LY +       +YDS EG         W+DDSD
Sbjct:  383 FKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDD--VELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSV-GQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSD 584          

HSP 3 Score: 71.2478 bits (173), Expect = 5.057e-11
Identity = 59/202 (29.21%), Postives = 98/202 (48.51%), Query Frame = 0
Query:  100 SQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLF--KRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIA--PDIIQGEDLKRTKILGYFFPCTLQIGDRFFY 297
            SQ G   ++ +  +  +  +N  +IY +NS+DL+ I  F+    +    W+G +  +K  + L   +G +RC ++    +   DV        LF      +YPG +L GP + F   +W S    +K ++S K  KF +V V + QV EL V W  ++     +D ++  P +I  E+L R K LG F     Q+G+R  Y
Sbjct:    6 SQCGTVIDVSIDCAVKLIGTN-CIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNG-ARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLS---GVKPVLSTK-SKF-RVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLY 200          
BLAST of EMLSAG00000000488 vs. GO
Match: - (symbol:UBE2O "Ubiquitin-conjugating enzyme E2 O" species:9606 "Homo sapiens" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005524 "ATP binding" evidence=IEA] [GO:0044822 "poly(A) RNA binding" evidence=IDA] InterPro:IPR000608 Pfam:PF00179 PROSITE:PS50127 UniPathway:UPA00143 GO:GO:0005524 Reactome:REACT_6900 EMBL:CH471099 GO:GO:0004842 Gene3D:3.10.110.10 InterPro:IPR016135 SUPFAM:SSF54495 eggNOG:COG5078 EMBL:AC090699 KO:K10581 EMBL:AB051521 EMBL:BC004525 EMBL:BC022237 EMBL:BC025977 EMBL:BC036820 EMBL:BC051868 EMBL:AL832432 EMBL:AK022940 EMBL:AK024657 EMBL:AK025999 RefSeq:NP_071349.3 UniGene:Hs.16130 ProteinModelPortal:Q9C0C9 SMR:Q9C0C9 BioGrid:121973 IntAct:Q9C0C9 PhosphoSite:Q9C0C9 DMDM:209572710 PaxDb:Q9C0C9 PRIDE:Q9C0C9 Ensembl:ENST00000319380 GeneID:63893 KEGG:hsa:63893 UCSC:uc002jrl.4 CTD:63893 GeneCards:GC17M074385 H-InvDB:HIX0020035 HGNC:HGNC:29554 HPA:HPA023605 neXtProt:NX_Q9C0C9 PharmGKB:PA142670651 HOVERGEN:HBG080116 InParanoid:Q9C0C9 OMA:DLQHIWP OrthoDB:EOG7GN2KX TreeFam:TF325556 SignaLink:Q9C0C9 ChiTaRS:UBE2O GeneWiki:UBE2O GenomeRNAi:63893 NextBio:65578 PRO:PR:Q9C0C9 Bgee:Q9C0C9 CleanEx:HS_UBE2O Genevestigator:Q9C0C9 Uniprot:Q9C0C9)

HSP 1 Score: 267.314 bits (682), Expect = 2.536e-72
Identity = 125/226 (55.31%), Postives = 162/226 (71.68%), Query Frame = 0
Query:  844 KNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISS 1069
            K   F + E AP  H+FK   F+P + K F   V+KE  LL++SLP GI++K +EDR+DLFSA+IKGP+ TPY DG++ FD QLP  YP  PP   Y+S CS RLNPNLY+ GKVCVSLLGTW GKGTE WT  S+LLQ+L+SIQGLILV EPY+NEAG++S +  + G ENS  YNEM +I++VQSMT+L+  PP +F+ EI +H +  G + V R   WL+  +
Sbjct:  925 KGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHA 1150          

HSP 2 Score: 130.954 bits (328), Expect = 2.291e-29
Identity = 81/205 (39.51%), Postives = 111/205 (54.15%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVV--RASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEE-SVYDLRDHPDFNYRPGSVVIKVANFDN----EESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKV------GEYDSDEGEL-------WDDDSD 583
             +PG+ V VE + T     V+WQDGS+E  I S++L+P+HHLD+ EF  G+FVV  R         YGVVQ  D+  R   +KWF     G D     +GE E+ SVYD+ DHPDF +R   +VI++ N ++    +E E  + G+V  V  + KV V WA+   T   PQ LY +       +YDS EG         W+DDSD
Sbjct:  538 FKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDD--VELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSV-GQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSD 739          

HSP 3 Score: 98.5969 bits (244), Expect = 1.970e-19
Identity = 78/248 (31.45%), Postives = 125/248 (50.40%), Query Frame = 0
Query:   54 LKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLF--KRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIA--PDIIQGEDLKRTKILGYFFPCTLQIGDRFFY 297
            L+ G V V WYP+  ++    + LKL +RS++P DVVR M S    SQ G   ++ +  +  +  +N  +IY +NS+DL+ I  F+    +    W+G +  +K  + L   +G +RC ++    +   DV        LF      +YPG +L GP + F   +W S    +K ++S K  KF +V V + QV EL V W  ++     +D ++  P +I  E+L R K LG F     Q+G+R  Y
Sbjct:  117 LRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRST--DSQCGTVIDVNIDCAVKLIGTN-CIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNG-ARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLS---GVKPVLSTK-SKF-RVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLY 355          
BLAST of EMLSAG00000000488 vs. GO
Match: - (symbol:UBE2O "Uncharacterized protein" species:9913 "Bos taurus" [GO:0016881 "acid-amino acid ligase activity" evidence=IEA] InterPro:IPR000608 Pfam:PF00179 PROSITE:PS50127 GO:GO:0008152 GO:GO:0016881 Gene3D:3.10.110.10 InterPro:IPR016135 SUPFAM:SSF54495 GeneTree:ENSGT00730000110680 KO:K10581 CTD:63893 OMA:DLQHIWP OrthoDB:EOG7GN2KX TreeFam:TF325556 EMBL:DAAA02049503 RefSeq:XP_002696213.1 UniGene:Bt.110870 Ensembl:ENSBTAT00000026793 GeneID:789422 KEGG:bta:789422 NextBio:20929675 ArrayExpress:F1N3I3 Uniprot:F1N3I3)

HSP 1 Score: 266.929 bits (681), Expect = 3.424e-72
Identity = 125/226 (55.31%), Postives = 162/226 (71.68%), Query Frame = 0
Query:  844 KNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISS 1069
            K   F + E AP  H+FK   F+P + K F   V+KE  LL++SLP GI++K +EDR+DLFSA+IKGP+ TPY DG++ FD QLP  YP  PP   Y+S CS RLNPNLY+ GKVCVSLLGTW GKGTE WT  S+LLQ+L+SIQGLILV EPY+NEAG++S +  + G ENS  YNEM +I++VQSMT+L+  PP +F+ EI +H +  G + V R   WL+  +
Sbjct:  924 KGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHA 1149          

HSP 2 Score: 131.339 bits (329), Expect = 1.709e-29
Identity = 81/205 (39.51%), Postives = 111/205 (54.15%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVV--RASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEE-SVYDLRDHPDFNYRPGSVVIKVANFDN----EESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKV------GEYDSDEGEL-------WDDDSD 583
             +PG+ V VE + T     V+WQDGS+E  I S++L+P+HHLD+ EF  G+FVV  R         YGVVQ  D+  R   +KWF     G D     +GE E+ SVYD+ DHPDF +R   +VI++ N ++    +E E  + G+V  V  + KV V WA+   T   PQ LY +       +YDS EG         W+DDSD
Sbjct:  536 FKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDD--VELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNAEDGAPHKEDEPSV-GQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSD 737          

HSP 3 Score: 98.2117 bits (243), Expect = 3.111e-19
Identity = 77/248 (31.05%), Postives = 125/248 (50.40%), Query Frame = 0
Query:   54 LKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLF--KRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIA--PDIIQGEDLKRTKILGYFFPCTLQIGDRFFY 297
            L+ G V V WYP+  ++    + LKL +RS++P DVVR M S    SQ G   ++ +  +  +  +N  ++Y +NS+DL+ I  F+    +    W+G +  +K  + L   +G +RC ++    +   DV        LF      +YPG +L GP + F   +W S    +K ++S K  KF +V V + QV EL V W  ++     +D ++  P +I  E+L R K LG F     Q+G+R  Y
Sbjct:  115 LRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTD--SQCGTVIDVNIDCAVKLIGTN-CIVYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNG-ARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLS---GVKPVLSTK-SKF-RVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLY 353          
BLAST of EMLSAG00000000488 vs. GO
Match: - (symbol:UBE2O "Uncharacterized protein" species:9823 "Sus scrofa" [GO:0016881 "acid-amino acid ligase activity" evidence=IEA] InterPro:IPR000608 Pfam:PF00179 PROSITE:PS50127 GO:GO:0008152 GO:GO:0016881 Gene3D:3.10.110.10 InterPro:IPR016135 SUPFAM:SSF54495 GeneTree:ENSGT00730000110680 OMA:DLQHIWP OrthoDB:EOG7GN2KX TreeFam:TF325556 EMBL:CU466934 Ensembl:ENSSSCT00000032146 Uniprot:I3L6C1)

HSP 1 Score: 266.544 bits (680), Expect = 3.704e-72
Identity = 125/226 (55.31%), Postives = 162/226 (71.68%), Query Frame = 0
Query:  844 KNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISS 1069
            K   F + E AP  H+FK   F+P + K F   V+KE  LL++SLP GI++K +EDR+DLFSA+IKGP+ TPY DG++ FD QLP  YP  PP   Y+S CS RLNPNLY+ GKVCVSLLGTW GKGTE WT  S+LLQ+L+SIQGLILV EPY+NEAG++S +  + G ENS  YNEM +I++VQSMT+L+  PP +F+ EI +H +  G + V R   WL+  +
Sbjct:  927 KGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVSRIESWLETHA 1152          

HSP 2 Score: 131.724 bits (330), Expect = 1.329e-29
Identity = 81/205 (39.51%), Postives = 111/205 (54.15%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVV--RASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEE-SVYDLRDHPDFNYRPGSVVIKVANFDN----EESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKV------GEYDSDEGEL-------WDDDSD 583
             +PG+ V VE + T     V+WQDGS+E  I S++L+P+HHLD+ EF  G+FVV  R         YGVVQ  D+  R   +KWF     G D     +GE E+ SVYD+ DHPDF +R   +VI++ N ++    +E E  + G+V  V  + KV V WA+   T   PQ LY +       +YDS EG         W+DDSD
Sbjct:  539 FKPGDRVAVEVVTTMTSADVMWQDGSVECSIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDD--VELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSV-GQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSD 740          

HSP 3 Score: 87.0409 bits (214), Expect = 7.569e-16
Identity = 71/225 (31.56%), Postives = 112/225 (49.78%), Query Frame = 0
Query:   77 LKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLF--KRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIA--PDIIQGEDLKRTKILGYFFPCTLQIGDRFFY 297
            LKL +RS++P DVVR M S    SQ G   ++ +  +  +  +N  +IY +NS DL+ I  F+    +    W+G +  +K  + L   +G +RC ++    +   DV        LF      +YPG +L GP + F   +W S    +K ++S K  KF +V V + QV EL V W  ++     +D ++  P +I  E+L R K LG F     Q+G+R  Y
Sbjct:  140 LKLEDRSVVPRDVVRHMRST--DSQCGTVMDVSIDCAVKLIGTN-CIIYPVNSRDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNG-ARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLS---GVKPVLSTK-SKF-RVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLY 355          
BLAST of EMLSAG00000000488 vs. GO
Match: - (symbol:UBE2O "Uncharacterized protein" species:9031 "Gallus gallus" [GO:0016881 "acid-amino acid ligase activity" evidence=IEA] InterPro:IPR000608 Pfam:PF00179 PROSITE:PS50127 GO:GO:0008152 GO:GO:0016881 Gene3D:3.10.110.10 InterPro:IPR016135 SUPFAM:SSF54495 GeneTree:ENSGT00730000110680 OrthoDB:EOG7GN2KX TreeFam:TF325556 EMBL:AADN03007513 EMBL:AADN03007522 Ensembl:ENSGALT00000003062 Uniprot:F1P5I5)

HSP 1 Score: 265.774 bits (678), Expect = 8.438e-72
Identity = 126/225 (56.00%), Postives = 158/225 (70.22%), Query Frame = 0
Query:  844 KNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZIS 1068
            K   F + E AP  H FK   F+P + K F   V+KE  LL++SLP GI++K +EDR+DLFSA+IKGP+ TPY DG+F FD QLP  YP  PP   Y+S CS RLNPNLY+ GKVCVSLLGTW GKGTE WT  S+LLQ+L+SIQGLILV EPY+NEAG++S +  + G ENS  YNEM +I++VQSM +LL  P  +F+ EI EH    G + V R   WL+ +
Sbjct:  916 KGEVFSVLECAPDSHAFKKMEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLFLFDIQLPNIYPAVPPLFRYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMTLIRVVQSMMQLLRRPVEVFEHEIREHFRCNGWRLVSRIESWLETN 1140          

HSP 2 Score: 131.724 bits (330), Expect = 1.558e-29
Identity = 84/214 (39.25%), Postives = 116/214 (54.21%), Query Frame = 0
Query:  398 KIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVV--RASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEE-SVYDLRDHPDFNYRPGSVVIKVANFDN---EESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKV------GEYDSDEGELWDDDSDFEGEEKDEVEEDSWVM 599
            + +PG+ V VE + T     V+WQDG++E  I S+EL P+HHLD+ EF  G+FVV  RA        YGVVQ  D+  R   +KWF     G D     +GE E+ SVYD+ DHPDF +R   +VI++ N D     E E  + G+V  V  + KV V WA+   T   PQ LY +       +YDS +G      S++E +E D  E D+ +M
Sbjct:  541 EFKPGDRVAVEVVTTMTSADVMWQDGTVETNIRSNELIPVHHLDNNEFCPGDFVVDKRAQSSQDPGVYGVVQSGDHIGRTCVVKWFKLKSSGDD--VELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNSDGATANEDEPSV-GQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVDGSTSGASSEWE-DESDSWETDNGLM 750          

HSP 3 Score: 87.0409 bits (214), Expect = 7.367e-16
Identity = 72/225 (32.00%), Postives = 112/225 (49.78%), Query Frame = 0
Query:   77 LKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLF--KRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIA--PDIIQGEDLKRTKILGYFFPCTLQIGDRFFY 297
            LKL +RS++P DVVR M S    SQ G   ++ +  +  +  +N  ++Y +NS+DL+ I  F+    +    W+G +  +K  V L   +G +RC +     +   DV        LF      +YPG +L GP + F   +W S    +K ++S K  KF +V V + QV EL V W  ++     +D ++  P II  E+L R K LG F     Q+G+R  Y
Sbjct:  134 LKLEDRSVVPRDVVRHMSS--SDSQCGTVIDVNIECAVKLVGTN-CILYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQVILKLSNG-ARCSMSTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPSKVFSSVQWLS---GVKPVLSTK-SKF-RVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSIISQENLSRVKRLGCFDHAQRQLGERCLY 349          
BLAST of EMLSAG00000000488 vs. C. finmarchicus
Match: gi|592938347|gb|GAXK01020206.1| (TSA: Calanus finmarchicus comp12403_c1_seq1 transcribed RNA sequence)

HSP 1 Score: 419.853 bits (1078), Expect = 2.917e-131
Identity = 266/651 (40.86%), Postives = 382/651 (58.68%), Query Frame = 0
Query:  506 RDHPDFNYRPGSVVIKVANFDNEESEGGLG-GEVIEVYPNGKVTVWWANKKTTE-----CWPQDLYKVGEYDSDEGELWDDDSXXXXXXXXXXXXXSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFN-------------------STSEFQNINAKENSLPSNDE-----QDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDI---------LLSITGLTYDDISP-----VLPKEEGEHKIEAXXXXXXXXXXXXXXXXXXXXXSLP--LEAVLNFKN----GTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPXXXXXXXXXXXXXXXXVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZIS-SSYHSNKKEISPL----------------DFPLLPAS 1089
            +DHPDF YRPGS+VI+V N   ++   GLG G+V++ +P+G+V+VWWA +   E     CWPQDLYKVGEYDSDEGELW+D+ +   E  ++    SW    +  G  +  K  E    E   +  +    EK R ++ +LEE FSE    QT  ++++L ++YKDC  L++ +GT +F   +F+ ++DR   + ++++  + AV+D ++RLF+                     S+ +  + K++   ++D      ++I     C ++CSL K +L   +  +V ++G ++       + +                L ++  L YD + P       P +E + + E  Q + V         +P    S    L+  L+  N    G+F + ++    H FK+++F+P + KNF+K V+KE KLL +SLP+GI +KGYEDR+DLFSAMI GP +TPY DG+FFFDFQL  DYP  PP+C YIS+C+DRLNPNLYEEGKVCVSLLGTWSGKGTE WTP+SNLLQLL+SIQGLILV+EPYFNEAGYE QK T+ GEENS MYNEM V+K+VQSMT+L+ +P   F  EI+EH  +   K + R   W +IS     S+   ++P                 DFPL+PAS
Sbjct:    1 KDHPDFKYRPGSIVIRVMNCAGDDC--GLGAGQVLDNHPSGQVSVWWAGEGENEGVRSACWPQDLYKVGEYDSDEGELWEDEDESGDESGND----SWETEEEQEGMEDV-KEEELHTDEMDIKPKLAAKIEKARIAMTRLEEIFSENPTLQTQGVMKQLLDVYKDCRYLDRLMGTEFFHEREFSGLLDRIRDQ-DRISSVEAAVKDHVNRLFSQEASPGKDSGNGSIVEESPGGSKVRGESPKKSDESNDDSAIAVGEEIPSSQVCGKLCSLIKTQLIKSHHEVVRRFGEVMEAKEEKAKEEETDIDLDDLDVLVARGLAALNFLPYDTVFPGCSMFRTPAKEDKPEDETAQDKPVDTPTTAVPATPDLDMSSSSILDKSLSLSNPLSPGSFVMLDAVADTHKFKLTIFQPNNIKNFVKHVRKEIKLLQTSLPAGITVKGYEDRMDLFSAMIHGPKNTPYEDGIFFFDFQLGCDYPTLPPSCHYISYCTDRLNPNLYEEGKVCVSLLGTWSGKGTETWTPDSNLLQLLVSIQGLILVREPYFNEAGYEKQKGTQQGEENSRMYNEMAVLKMVQSMTKLVRNPSQAFAKEIMEHWRDNAGKLMTRLMMWKKISLGQLQSSATPVTPTTPGIFANLTTGIVDLPDFPLVPAS 1929          
BLAST of EMLSAG00000000488 vs. C. finmarchicus
Match: gi|592938346|gb|GAXK01020207.1| (TSA: Calanus finmarchicus comp12403_c1_seq2 transcribed RNA sequence)

HSP 1 Score: 405.986 bits (1042), Expect = 8.109e-127
Identity = 252/593 (42.50%), Postives = 361/593 (60.88%), Query Frame = 0
Query:  506 RDHPDFNYRPGSVVIKVANFDNEESEGGLG-GEVIEVYPNGKVTVWWANKKTTE-----CWPQDLYKVGEYDSDEGELWDDDSXXXXXXXXXXXXXSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFN-------------------STSEFQNINAKENSLPSNDE-----QDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDI---------LLSITGLTYDDISP-----VLPKEEGEHKIEAXXXXXXXXXXXXXXXXXXXXXSLP--LEAVLNFKN----GTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPXXXXXXXXXXXXXXXXVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILE 1048
            +DHPDF YRPGS+VI+V N   ++   GLG G+V++ +P+G+V+VWWA +   E     CWPQDLYKVGEYDSDEGELW+D+ +   E  ++    SW    +  G  +  K  E    E   +  +    EK R ++ +LEE FSE    QT  ++++L ++YKDC  L++ +GT +F   +F+ ++DR   + ++++  + AV+D ++RLF+                     S+ +  + K++   ++D      ++I     C ++CSL K +L   +  +V ++G ++       + +                L ++  L YD + P       P +E + + E  Q + V         +P    S    L+  L+  N    G+F + ++    H FK+++F+P + KNF+K V+KE KLL +SLP+GI +KGYEDR+DLFSAMI GP +TPY DG+FFFDFQL  DYP  PP+C YIS+C+DRLNPNLYEEGKVCVSLLGTWSGKGTE WTP+SNLLQLL+SIQGLILV+EPYFNEAGYE QK T+ GEENS MYNEM V+K+VQSMT+L+ +PP  F  EI+E
Sbjct:    1 KDHPDFKYRPGSIVIRVMNCAGDDC--GLGAGQVLDNHPSGQVSVWWAGEGENEGVRSACWPQDLYKVGEYDSDEGELWEDEDESGDESGND----SWETEEEQEGMEDV-KEEELHTDEMDIKPKLAAKIEKARIAMTRLEEIFSENPTLQTQGVMKQLLDVYKDCRYLDRLMGTEFFHEREFSGLLDRIRDQ-DRISSVEAAVKDHVNRLFSQEASPGKDSGNGSIVEESPGGSKVRGESPKKSDESNDDSAIAVGEEIPSSQVCGKLCSLIKTQLIKSHHEVVRRFGEVMEAKEEKAKEEETDIDLDDLDVLVARGLAALNFLPYDTVFPGCSMFRTPAKEDKPEDETAQDKPVDTPTTAVPATPDLDMSSSSILDKSLSLSNPLSPGSFVMLDAVADTHKFKLTIFQPNNIKNFVKHVRKEIKLLQTSLPAGITVKGYEDRMDLFSAMIHGPKNTPYEDGIFFFDFQLGCDYPTLPPSCHYISYCTDRLNPNLYEEGKVCVSLLGTWSGKGTETWTPDSNLLQLLVSIQGLILVREPYFNEAGYEKQKGTQQGEENSRMYNEMAVLKMVQSMTKLVRNPPQPFAKEIME 1755          
BLAST of EMLSAG00000000488 vs. C. finmarchicus
Match: gi|592938335|gb|GAXK01020218.1| (TSA: Calanus finmarchicus comp12403_c3_seq1 transcribed RNA sequence)

HSP 1 Score: 343.584 bits (880), Expect = 8.522e-100
Identity = 212/567 (37.39%), Postives = 320/567 (56.44%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLG-GEVIEVYPNGKVTVWWANKKTTE-----CWPQDLYKVGEYDSDEGELWDDDSXXXXXXXXXXXXXSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFN-------------------STSEFQNINAKENSLPSNDE-----QDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDI---------LLSITGLTYDDISP-----VLPKEEGEHKIEAXXXXXXXXXXXXXXXXXXXXXSLP--LEAVLNFKN----GTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTP 915
            ++PG+ VV E L T++EV V+WQDGS+E+ I S+ELYPIHHLDD EFF G+FV  A +     SYGVVQ VD+  R  K+KWF TY  G+ P P YV  ++ S YDL+DHPDF YRPGS+VI+V N   ++   GLG G+V++ +P+G+V+VWWA +   E     CWPQDLYKVGEYDSDEGELW+D+     E  DE   DSW    +  G  +  K  E    E   +  +    EK R ++ +LEE FSE    QT  ++++L ++YKDC  L++ +GT +F   +F+ ++DR   + ++++  + AV+D ++RLF+                     S+ +  + K++   ++D      ++I     C ++CSL K +L   +  +V ++G ++       + +                L ++  L YD + P       P +E + + E  Q + V         +P    S    L+  L+  N    G+F + ++    H FK+++F+P + KNF+K V+KE KLL +SLP+GI +KGYEDR+DLFSAMI GP +TP
Sbjct:    3 VKPGDKVVTETLSTRSEVDVVWQDGSVEKAIPSAELYPIHHLDDHEFFPGDFVTEAKEGFQPHSYGVVQDVDHAGRCCKVKWFRTYTEGAQPQPIYVSTADASAYDLKDHPDFKYRPGSIVIRVMNCAGDDC--GLGAGQVLDNHPSGQVSVWWAGEGENEGVRSACWPQDLYKVGEYDSDEGELWEDED----ESGDESGNDSWETEEEQEGMEDV-KEEELHTDEMDIKPKLAAKIEKARIAMTRLEEIFSENPTLQTQGVMKQLLDVYKDCRYLDRLMGTEFFHEREFSGLLDRIRDQ-DRISSVEAAVKDHVNRLFSQEASPGKDSGNGSIVEESPGGSKVRGESPKKSDESNDDSAIAVGEEIPSSQVCGKLCSLIKTQLIKSHHEVVRRFGEVMEAKEEKAKEEETDIDLDDLDVLVARGLAALNFLPYDTVFPGCSMFRTPAKEDKPEDETAQDKPVDTPTTAVPATPDLDMSSSSILDKSLSLSNPLSPGSFVMLDAVADTHKFKLTIFQPNNIKNFVKHVRKEIKLLQTSLPAGITVKGYEDRMDLFSAMIHGPKNTP 1679          

HSP 2 Score: 241.506 bits (615), Expect = 2.984e-65
Identity = 130/312 (41.67%), Postives = 189/312 (60.58%), Query Frame = 0
Query:   13 FHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQA----CVGNESDGIAPDI-IQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREIL 319
            F+EDEV+ + KKG + FG V+ + E  +    E  DE+   +K G++ V+WYP+  E+V     + L +RSLMPGDVVRR+   GK +Q GYCR++KV +S  +  +N +VI+ + SEDL P+++F ++  + L  WVG++R VK N+ L F DG S+ +VDD V    DD+ DK      FKR D+YPG IL GP++  EG +W  CS  ++     KP K  KVTV +     L V+W C+A        E     P   ++GE L R K+L  F PCTLQIGDR FY +KE D+++ KS+W + +++ L
Sbjct: 1950 FYEDEVFRITKKGALQFGMVIENAEFASS--DESSDEEEQPVKAGQIRVAWYPQGSEEVVPERKVHLADRSLMPGDVVRRL-VQGKNTQRGYCRDVKVYSSVQVIGTN-QVIFGVQSEDLTPLEAFKSDILIGLDGWVGMVRSVKSNLTLRFTDG-SKVIVDDEVAEDLDDIRDKRDPECEFKRYDFYPGQILFGPVKSLEGGQWIECSPDIQSTRKHKPHKAFKVTVEEIDFISLGVSWICRAYFSSTTSTEETKAQPKFKVEGESLARVKMLNVFEPCTLQIGDRNFYTMKENDIVMMKSEWKKLQKDQL 2870          
BLAST of EMLSAG00000000488 vs. C. finmarchicus
Match: gi|592938343|gb|GAXK01020210.1| (TSA: Calanus finmarchicus comp12403_c1_seq5 transcribed RNA sequence)

HSP 1 Score: 289.656 bits (740), Expect = 9.863e-88
Identity = 155/261 (59.39%), Postives = 192/261 (73.56%), Query Frame = 0
Query:  846 GTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPXXXXXXXXXXXXXXXXVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZIS-SSYHSNKKEISPL----------------DFPLLPAS 1089
            G+F + ++    H FK+++F+P + KNF+K V+KE KLL +SLP+GI +KGYEDR+DLFSAMI GP +TPY DG+FFFDFQL  DYP  PP+C YIS+C+DRLNPNLYEEGKVCVSLLGTWSGKGTE WTP+SNLLQLL+SIQGLILV+EPYFNEAGYE QK T+ GEENS MYNEM V+K+VQSMT+L+ +P   F  EI+EH  +   K + R   W +IS     S+   ++P                 DFPL+PAS
Sbjct:  100 GSFVMLDAVADTHKFKLTIFQPNNIKNFVKHVRKEIKLLQTSLPAGITVKGYEDRMDLFSAMIHGPKNTPYEDGIFFFDFQLGCDYPTLPPSCHYISYCTDRLNPNLYEEGKVCVSLLGTWSGKGTETWTPDSNLLQLLVSIQGLILVREPYFNEAGYEKQKGTQQGEENSRMYNEMAVLKMVQSMTKLVRNPSQAFAKEIMEHWRDNAGKLMTRLMMWKKISLGQLQSSATPVTPTTPGIFANLTTGIVDLPDFPLVPAS 882          
BLAST of EMLSAG00000000488 vs. C. finmarchicus
Match: gi|592938334|gb|GAXK01020219.1| (TSA: Calanus finmarchicus comp12403_c3_seq2 transcribed RNA sequence)

HSP 1 Score: 305.064 bits (780), Expect = 1.567e-86
Identity = 195/546 (35.71%), Postives = 300/546 (54.95%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLG-GEVIEVYPNGKVTVWWANKKTTE-----CWPQDLYKVGEYDSDEGELWDDDSXXXXXXXXXXXXXSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFN-------------------STSEFQNINAKENSLPSNDE-----QDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDI---------LLSITGLTYDDISP-----VLPKEEGEHKIEAXXXXXXXXXXXXXXXXXXXXXSLP--LEAVLNFKN----GTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVL 894
            ++PG+ VV E L T++EV V+WQDGS+E+ I S+ELYPIHHLDD EFF G+FV  A +     SYGVVQ VD+  R  K+KWF TY  G+ P P YV  ++ S YDL+DHPDF YRPGS+VI+V N   ++   GLG G+V++ +P+G+V+VWWA +   E     CWPQDLYKVGEYDSDEGELW+D+     E  DE   DSW    +  G  +  K  E    E   +  +    EK R ++ +LEE FSE    QT  ++++L ++YKDC  L++ +GT +F   +F+ ++DR   + ++++  + AV+D ++RLF+                     S+ +  + K++   ++D      ++I     C ++CSL K +L   +  +V ++G ++       + +                L ++  L YD + P       P +E + + E  Q + V         +P    S    L+  L+  N    G+F + ++    H FK+++F+P + KNF+K V+KE KLL +SLP GI +
Sbjct:    1 VKPGDKVVTETLSTRSEVDVVWQDGSVEKAIPSAELYPIHHLDDHEFFPGDFVTEAKEGFQPHSYGVVQDVDHAGRCCKVKWFRTYTEGAQPQPIYVSTADASAYDLKDHPDFKYRPGSIVIRVMNCAGDDC--GLGAGQVLDNHPSGQVSVWWAGEGENEGVRSACWPQDLYKVGEYDSDEGELWEDED----ESGDESGNDSWETEEEQEGMEDV-KEEELHTDEMDIKPKLAAKIEKARIAMTRLEEIFSENPTLQTQGVMKQLLDVYKDCRYLDRLMGTEFFHEREFSGLLDRIRDQ-DRISSVEAAVKDHVNRLFSQEASPGKDSGNGSIVEESPGGSKVRGESPKKSDESNDDSAIAVGEEIPSSQVCGKLCSLIKTQLIKSHHEVVRRFGEVMEAKEEKAKEEETDIDLDDLDVLVARGLAALNFLPYDTVFPGCSMFRTPAKEDKPEDETAQDKPVDTPTTAVPATPDLDMSSSSILDKSLSLSNPLSPGSFVMLDAVADTHKFKLTIFQPNNIKNFVKHVRKEIKLLQTSLPPGITV 1614          

HSP 2 Score: 241.121 bits (614), Expect = 3.006e-65
Identity = 130/312 (41.67%), Postives = 189/312 (60.58%), Query Frame = 0
Query:   13 FHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQA----CVGNESDGIAPDI-IQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREIL 319
            F+EDEV+ + KKG + FG V+ + E  +    E  DE+   +K G++ V+WYP+  E+V     + L +RSLMPGDVVRR+   GK +Q GYCR++KV +S  +  +N +VI+ + SEDL P+++F ++  + L  WVG++R VK N+ L F DG S+ +VDD V    DD+ DK      FKR D+YPG IL GP++  EG +W  CS  ++     KP K  KVTV +     L V+W C+A        E     P   ++GE L R K+L  F PCTLQIGDR FY +KE D+++ KS+W + +++ L
Sbjct: 1885 FYEDEVFRITKKGALQFGMVIENAEFASS--DESSDEEEQPVKAGQIRVAWYPQGSEEVVPERKVHLADRSLMPGDVVRRL-VQGKNTQRGYCRDVKVYSSVQVIGTN-QVIFGVQSEDLTPLEAFKSDILIGLDGWVGMVRSVKSNLTLRFTDG-SKVIVDDEVAEDLDDIRDKRDPECEFKRYDFYPGQILFGPVKSLEGGQWIECSPDIQSTRKHKPHKAFKVTVEEIDFISLGVSWICRAYFSSTTSTEETKAQPKFKVEGESLARVKMLNVFEPCTLQIGDRNFYTMKENDIVMMKSEWKKLQKDQL 2805          
BLAST of EMLSAG00000000488 vs. C. finmarchicus
Match: gi|592938341|gb|GAXK01020212.1| (TSA: Calanus finmarchicus comp12403_c1_seq7 transcribed RNA sequence)

HSP 1 Score: 275.018 bits (702), Expect = 2.662e-83
Identity = 141/203 (69.46%), Postives = 171/203 (84.24%), Query Frame = 0
Query:  846 GTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPXXXXXXXXXXXXXXXXVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILE 1048
            G+F + ++    H FK+++F+P + KNF+K V+KE KLL +SLP+GI +KGYEDR+DLFSAMI GP +TPY DG+FFFDFQL  DYP  PP+C YIS+C+DRLNPNLYEEGKVCVSLLGTWSGKGTE WTP+SNLLQLL+SIQGLILV+EPYFNEAGYE QK T+ GEENS MYNEM V+K+VQSMT+L+ +PP  F  EI+E
Sbjct:  100 GSFVMLDAVADTHKFKLTIFQPNNIKNFVKHVRKEIKLLQTSLPAGITVKGYEDRMDLFSAMIHGPKNTPYEDGIFFFDFQLGCDYPTLPPSCHYISYCTDRLNPNLYEEGKVCVSLLGTWSGKGTETWTPDSNLLQLLVSIQGLILVREPYFNEAGYEKQKGTQQGEENSRMYNEMAVLKMVQSMTKLVRNPPQPFAKEIME 708          
BLAST of EMLSAG00000000488 vs. C. finmarchicus
Match: gi|592938333|gb|GAXK01020220.1| (TSA: Calanus finmarchicus comp12403_c3_seq3 transcribed RNA sequence)

HSP 1 Score: 278.87 bits (712), Expect = 2.840e-78
Identity = 158/400 (39.50%), Postives = 238/400 (59.50%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLG-GEVIEVYPNGKVTVWWANKKTTE-----CWPQDLYKVGEYDSDEGELWDDDSXXXXXXXXXXXXXSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFN-------------------STSEFQNINAKENSLPSNDE-----QDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMV 768
            ++PG+ VV E L T++EV V+WQDGS+E+ I S+ELYPIHHLDD EFF G+FV  A +     SYGVVQ VD+  R  K+KWF TY  G+ P P YV  ++ S YDL+DHPDF YRPGS+VI+V N   ++   GLG G+V++ +P+G+V+VWWA +   E     CWPQDLYKVGEYDSDEGELW+D+     E  DE   DSW    +  G  +  K  E    E   +  +    EK R ++ +LEE FSE    QT  ++++L ++YKDC  L++ +GT +F   +F+ ++DR   + ++++  + AV+D ++RLF+                     S+ +  + K++   ++D      ++I     C ++CSL K +L   +  +V ++G ++
Sbjct:  129 VKPGDKVVTETLSTRSEVDVVWQDGSVEKAIPSAELYPIHHLDDHEFFPGDFVTEAKEGFQPHSYGVVQDVDHAGRCCKVKWFRTYTEGAQPQPIYVSTADASAYDLKDHPDFKYRPGSIVIRVMNCAGDDC--GLGAGQVLDNHPSGQVSVWWAGEGENEGVRSACWPQDLYKVGEYDSDEGELWEDED----ESGDESGNDSWETEEEQEGMEDV-KEEELHTDEMDIKPKLAAKIEKARIAMTRLEEIFSENPTLQTQGVMKQLLDVYKDCRYLDRLMGTEFFHEREFSGLLDRIRDQ-DRISSVEAAVKDHVNRLFSQEASPGKDSGNGSIVEESPGGSKVRGESPKKSDESNDDSAIAVGEEIPSSQVCGKLCSLIKTQLIKSHHEVVRRFGEVM 1304          

HSP 2 Score: 240.35 bits (612), Expect = 2.490e-65
Identity = 130/314 (41.40%), Postives = 190/314 (60.51%), Query Frame = 0
Query:   11 QLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQA----CVGNESDGIAPDI-IQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREIL 319
            + F+EDEV+ + KKG + FG V+ + E  +    E  DE+   +K G++ V+WYP+  E+V     + L +RSLMPGDVVRR+   GK +Q GYCR++KV +S  +  +N +VI+ + SEDL P+++F ++  + L  WVG++R VK N+ L F DG S+ +VDD V    DD+ DK      FKR D+YPG IL GP++  EG +W  CS  ++     KP K  KVTV +     L V+W C+A        E     P   ++GE L R K+L  F PCTLQIGDR FY +KE D+++ KS+W + +++ L
Sbjct: 1575 KYFYEDEVFRITKKGALQFGMVIENAEFASS--DESSDEEEQPVKAGQIRVAWYPQGSEEVVPERKVHLADRSLMPGDVVRRL-VQGKNTQRGYCRDVKVYSSVQVIGTN-QVIFGVQSEDLTPLEAFKSDILIGLDGWVGMVRSVKSNLTLRFTDG-SKVIVDDEVAEDLDDIRDKRDPECEFKRYDFYPGQILFGPVKSLEGGQWIECSPDIQSTRKHKPHKAFKVTVEEIDFISLGVSWICRAYFSSTTSTEETKAQPKFKVEGESLARVKMLNVFEPCTLQIGDRNFYTMKENDIVMMKSEWKKLQKDQL 2501          
BLAST of EMLSAG00000000488 vs. C. finmarchicus
Match: gi|592938326|gb|GAXK01020227.1| (TSA: Calanus finmarchicus comp12403_c3_seq10 transcribed RNA sequence)

HSP 1 Score: 252.677 bits (644), Expect = 1.054e-75
Identity = 137/219 (62.56%), Postives = 162/219 (73.97%), Query Frame = 0
Query:  895 KGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPXXXXXXXXXXXXXXXXVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZIS-SSYHSNKKEISPL----------------DFPLLPASKGFCVSF 1096
            KGYEDR+DLFSAMI GP +TPY DG+FFFDFQL  DYP  PP+C YIS+C+DRLNPNLYEEGKVCVSLLGTWSGKGTE WTP+SNLLQLL+SIQGLILV+EPYFNEAGYE QK T+ GEENS MYNEM V+K+VQSMT+L+ +P   F  EI+EH  +   K + R   W +IS     S+   ++P                 DFPL+PASKGFC++ 
Sbjct:    1 KGYEDRMDLFSAMIHGPKNTPYEDGIFFFDFQLGCDYPTLPPSCHYISYCTDRLNPNLYEEGKVCVSLLGTWSGKGTETWTPDSNLLQLLVSIQGLILVREPYFNEAGYEKQKGTQQGEENSRMYNEMAVLKMVQSMTKLVRNPSQAFAKEIMEHWRDNAGKLMTRLMMWKKISLGQLQSSATPVTPTTPGIFANLTTGIVDLPDFPLVPASKGFCLTL 657          
BLAST of EMLSAG00000000488 vs. C. finmarchicus
Match: gi|592938340|gb|GAXK01020213.1| (TSA: Calanus finmarchicus comp12403_c1_seq8 transcribed RNA sequence)

HSP 1 Score: 245.743 bits (626), Expect = 2.897e-73
Identity = 135/216 (62.50%), Postives = 159/216 (73.61%), Query Frame = 0
Query:  891 GIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPXXXXXXXXXXXXXXXXVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZIS-SSYHSNKKEISPL----------------DFPLLPAS 1089
            GI +KGYEDR+DLFSAMI GP +TPY DG+FFFDFQL  DYP  PP+C YIS+C+DRLNPNLYEEGKVCVSLLGTWSGKGTE WTP+SNLLQLL+SIQGLILV+EPYFNEAGYE QK T+ GEENS MYNEM V+K+VQSMT+L+ +P   F  EI+EH  +   K + R   W +IS     S+   ++P                 DFPL+PAS
Sbjct:    4 GITVKGYEDRMDLFSAMIHGPKNTPYEDGIFFFDFQLGCDYPTLPPSCHYISYCTDRLNPNLYEEGKVCVSLLGTWSGKGTETWTPDSNLLQLLVSIQGLILVREPYFNEAGYEKQKGTQQGEENSRMYNEMAVLKMVQSMTKLVRNPSQAFAKEIMEHWRDNAGKLMTRLMMWKKISLGQLQSSATPVTPTTPGIFANLTTGIVDLPDFPLVPAS 651          
BLAST of EMLSAG00000000488 vs. C. finmarchicus
Match: gi|592938339|gb|GAXK01020214.1| (TSA: Calanus finmarchicus comp12403_c1_seq9 transcribed RNA sequence)

HSP 1 Score: 241.121 bits (614), Expect = 1.199e-71
Identity = 133/212 (62.74%), Postives = 156/212 (73.58%), Query Frame = 0
Query:  895 KGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPXXXXXXXXXXXXXXXXVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZIS-SSYHSNKKEISPL----------------DFPLLPAS 1089
            KGYEDR+DLFSAMI GP +TPY DG+FFFDFQL  DYP  PP+C YIS+C+DRLNPNLYEEGKVCVSLLGTWSGKGTE WTP+SNLLQLL+SIQGLILV+EPYFNEAGYE QK T+ GEENS MYNEM V+K+VQSMT+L+ +P   F  EI+EH  +   K + R   W +IS     S+   ++P                 DFPL+PAS
Sbjct:    1 KGYEDRMDLFSAMIHGPKNTPYEDGIFFFDFQLGCDYPTLPPSCHYISYCTDRLNPNLYEEGKVCVSLLGTWSGKGTETWTPDSNLLQLLVSIQGLILVREPYFNEAGYEKQKGTQQGEENSRMYNEMAVLKMVQSMTKLVRNPSQAFAKEIMEHWRDNAGKLMTRLMMWKKISLGQLQSSATPVTPTTPGIFANLTTGIVDLPDFPLVPAS 636          
BLAST of EMLSAG00000000488 vs. L. salmonis peptides
Match: EMLSAP00000000488 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1075:36709:41130:-1 gene:EMLSAG00000000488 transcript:EMLSAT00000000488 description:"maker-LSalAtl2s1075-augustus-gene-0.30")

HSP 1 Score: 2280.75 bits (5909), Expect = 0.000e+0
Identity = 1115/1115 (100.00%), Postives = 1115/1115 (100.00%), Query Frame = 0
Query:    1 MSRGREEEIAQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIAPDIIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREILANEMDGESKPVDSIKLKEFDVKKEEAESKSLSVPNNSINKSNVSISVNKLRRRLKKRNKSVTAELYSNLPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFNSTSEFQNINAKENSLPSNDEQDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKIEAPQVEKVVVSPPEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPLDFPLLPASKGFCVSFAKSLDEFDLAHSKYLASNS 1115
            MSRGREEEIAQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIAPDIIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREILANEMDGESKPVDSIKLKEFDVKKEEAESKSLSVPNNSINKSNVSISVNKLRRRLKKRNKSVTAELYSNLPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFNSTSEFQNINAKENSLPSNDEQDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKIEAPQVEKVVVSPPEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPLDFPLLPASKGFCVSFAKSLDEFDLAHSKYLASNS
Sbjct:    1 MSRGREEEIAQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIAPDIIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREILANEMDGESKPVDSIKLKEFDVKKEEAESKSLSVPNNSINKSNVSISVNKLRRRLKKRNKSVTAELYSNLPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFNSTSEFQNINAKENSLPSNDEQDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKIEAPQVEKVVVSPPEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPLDFPLLPASKGFCVSFAKSLDEFDLAHSKYLASNS 1115          
BLAST of EMLSAG00000000488 vs. L. salmonis peptides
Match: EMLSAP00000001497 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1257:2789:4309:1 gene:EMLSAG00000001497 transcript:EMLSAT00000001497 description:"maker-LSalAtl2s1257-augustus-gene-0.7")

HSP 1 Score: 70.0922 bits (170), Expect = 1.575e-13
Identity = 44/151 (29.14%), Postives = 70/151 (46.36%), Query Frame = 0
Query:  877 VKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEP----------YFNEAGYESQKFTKHGEENSW 1017
            + ++ K L    P+G+     E+ I +++A+I GP DTP+ DG F    +   +YP  PP    + F S   +PN+Y +G +C+ +L          W+P  ++  +L SIQ L+    P           F E   E +K  K   E SW
Sbjct:    9 LMRDFKRLQEDPPAGVSGAPGENNIMVWNAVIFGPHDTPFEDGTFKLTMEFSEEYPNKPP---LVKFLSKMFHPNIYADGGICLDIL-------QNRWSPTYDVSAILTSIQSLLSDPNPNSPANSMAAQLFKENRREYEKRVKACVEQSW 149          
BLAST of EMLSAG00000000488 vs. L. salmonis peptides
Match: EMLSAP00000008755 (pep:novel supercontig:LSalAtl2s:LSalAtl2s543:247950:248568:-1 gene:EMLSAG00000008755 transcript:EMLSAT00000008755 description:"maker-LSalAtl2s543-augustus-gene-1.70")

HSP 1 Score: 69.707 bits (169), Expect = 1.927e-13
Identity = 43/151 (28.48%), Postives = 71/151 (47.02%), Query Frame = 0
Query:  879 KEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEP----------YFNEAGYESQKFTKHGEENSWMY 1019
            ++ K L    P+G+     ++ I L++A+I GP DTP+ DG F        +YP  PPT   + F S   +PN+Y +G +C+ +L          W+P  ++  +L SIQ L+    P           + E   E +K  +   E SW++
Sbjct:   11 RDFKRLQEDPPAGVSGAPGDNNIMLWNAVIFGPHDTPFEDGTFKLTIGFTEEYPNKPPT---VRFVSKMFHPNVYADGGICLDIL-------QNRWSPTYDVSAILTSIQSLLDXPNPNSPANSVAAQLYQENRREYEKRVQAIVEQSWLH 151          
BLAST of EMLSAG00000000488 vs. L. salmonis peptides
Match: EMLSAP00000007154 (pep:novel supercontig:LSalAtl2s:LSalAtl2s403:128256:128762:1 gene:EMLSAG00000007154 transcript:EMLSAT00000007154 description:"augustus_masked-LSalAtl2s403-processed-gene-1.1")

HSP 1 Score: 68.5514 bits (166), Expect = 5.323e-13
Identity = 42/125 (33.60%), Postives = 65/125 (52.00%), Query Frame = 0
Query:  874 LKFVKKEAKLLSSSLPSGIVLKGY-EDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSG------KGTEIWTPNSNLLQLLLSIQGLI 991
            L+ +  E K L  + P GI+     ED    + A I GP DTP+ DGVF      P DYP +PP    + F +   +PN+Y +G+VC+S+L +            E W+P  ++ ++LLS+  L+
Sbjct:    6 LRRLIAEFKQLWDNPPEGILAGPMSEDNYLEWEACITGPEDTPFADGVFVARLSFPKDYPLNPPK---MKFITPIFHPNIYSDGRVCISILHSPGDDPLGYETSAERWSPVQSVEKVLLSVMSLL 127          
BLAST of EMLSAG00000000488 vs. L. salmonis peptides
Match: EMLSAP00000007076 (pep:novel supercontig:LSalAtl2s:LSalAtl2s3:487621:488064:-1 gene:EMLSAG00000007076 transcript:EMLSAT00000007076 description:"augustus_masked-LSalAtl2s3-processed-gene-4.0")

HSP 1 Score: 64.3142 bits (155), Expect = 9.507e-12
Identity = 38/123 (30.89%), Postives = 57/123 (46.34%), Query Frame = 0
Query:  874 LKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEP 996
            LK + KE   L    P+        D +  + A I GP D+PY  GVFF     P DYP  PP    ++F +   +PN+   G +C+ +L +        W+P   + ++LLSI  L+    P
Sbjct:    3 LKRINKELVDLGRDPPAQCSAGPVGDDLFHWQATIMGPPDSPYQGGVFFLTIHFPTDYPFKPPK---VAFATRIYHPNINSNGSICLDILRSQ-------WSPALTVAKVLLSICSLLCDPNP 115          
BLAST of EMLSAG00000000488 vs. L. salmonis peptides
Match: EMLSAP00000006800 (pep:novel supercontig:LSalAtl2s:LSalAtl2s380:822042:827984:-1 gene:EMLSAG00000006800 transcript:EMLSAT00000006800 description:"maker-LSalAtl2s380-augustus-gene-8.15")

HSP 1 Score: 61.6178 bits (148), Expect = 7.624e-11
Identity = 36/122 (29.51%), Postives = 61/122 (50.00%), Query Frame = 0
Query:  875 KFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEP 996
            + VK+  +L+   +P GI     E     F  ++ GP ++P+  GVF  +  LP DYP S P    + F +   +PN+ + G++C+ +L        + W+P   +  +LLSIQ L+    P
Sbjct:    7 RIVKETQRLMKEPVP-GISAIPDEGNARYFHVVVAGPEESPFEGGVFKLELFLPEDYPMSAPK---VRFITKIYHPNIDKLGRICLDIL-------KDKWSPALQIRTVLLSIQALLSAPNP 117          
BLAST of EMLSAG00000000488 vs. L. salmonis peptides
Match: EMLSAP00000003004 (pep:novel supercontig:LSalAtl2s:LSalAtl2s170:743180:756466:-1 gene:EMLSAG00000003004 transcript:EMLSAT00000003004 description:"maker-LSalAtl2s170-snap-gene-7.23")

HSP 1 Score: 56.6102 bits (135), Expect = 3.137e-9
Identity = 37/121 (30.58%), Postives = 62/121 (51.24%), Query Frame = 0
Query:  877 VKKEAKLLSSSLPSGIVLKGYED-RIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEP 996
            +KKE ++L  + P G+     ++  +    A I G  DTPY  G+F     +P  YP  PP    + F +   +PN+   G++C+ LL     KG+  W  + N+  +L+SI+  +L+ EP
Sbjct:    7 IKKELEMLXKNPPPGVSCAAPDNGNLTSLEANILGSEDTPYEGGLFKLSIHIPETYPFQPPI---VQFKTRIYHPNVDAAGRICLDLLKI-PPKGS--WKASLNISSILMSIR--LLMNEP 119          
BLAST of EMLSAG00000000488 vs. L. salmonis peptides
Match: EMLSAP00000006521 (pep:novel supercontig:LSalAtl2s:LSalAtl2s357:29932:41534:-1 gene:EMLSAG00000006521 transcript:EMLSAT00000006521 description:"maker-LSalAtl2s357-snap-gene-0.5")

HSP 1 Score: 55.8398 bits (133), Expect = 7.314e-9
Identity = 35/88 (39.77%), Postives = 47/88 (53.41%), Query Frame = 0
Query:  910 GPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLL-----GTWSGK-GTEIWTPNSNLLQLLLSIQGLI 991
            GP DT Y  G F    + P DYP SPPT   + F S   +PN+YE G +C+S+L        SG+   E W P  N+  +LLS+  L+
Sbjct:   50 GPPDTLYQGGYFKALVKFPSDYPYSPPT---VRFISKVWHPNVYENGDLCISILHPPVDDPQSGELPCERWNPTQNVRTILLSVVSLL 134          
BLAST of EMLSAG00000000488 vs. L. salmonis peptides
Match: EMLSAP00000004972 (pep:novel supercontig:LSalAtl2s:LSalAtl2s260:1392821:1393285:1 gene:EMLSAG00000004972 transcript:EMLSAT00000004972 description:"augustus_masked-LSalAtl2s260-processed-gene-14.9")

HSP 1 Score: 52.373 bits (124), Expect = 7.693e-8
Identity = 30/100 (30.00%), Postives = 52/100 (52.00%), Query Frame = 0
Query:  911 PSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTK 1010
            P   PY+ G F  +   PP+YP  PP    I+F +   +PN+ E+G+VC+ ++        + W P +   Q++L++  LI   EP     G  +++FT+
Sbjct:   41 PDKYPYNKGAFKVEITFPPEYPFKPPK---INFKTKIYHPNVXEKGQVCLPII------LADNWKPATKTNQVVLALVSLINEPEPEHPLRGDLAKEFTR 131          
BLAST of EMLSAG00000000488 vs. L. salmonis peptides
Match: EMLSAP00000013079 (pep:novel supercontig:LSalAtl2s:LSalAtl2s9:891889:896824:1 gene:EMLSAG00000013079 transcript:EMLSAT00000013079 description:"snap_masked-LSalAtl2s9-processed-gene-9.12")

HSP 1 Score: 50.447 bits (119), Expect = 3.262e-7
Identity = 34/128 (26.56%), Postives = 58/128 (45.31%), Query Frame = 0
Query:  875 KFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAG 1002
            K ++KE   ++   P         D I  + + I GP  + Y  GVFF D    P+YP  PP    ++F +   + N+  +G +C+ +L        + W+P   + ++LLS+  L+    P F   G
Sbjct:   42 KRIQKELAEITLDPPPNCSAGPKGDNIYEWVSTILGPPGSVYEGGVFFLDIHFSPEYPFRPPK---VTFRTRIYHCNINSQGVICLDIL-------KDNWSPALTVSKVLLSVCSLLTDCNPGFGSLG 159          
BLAST of EMLSAG00000000488 vs. SwissProt
Match: gi|342187119|sp|Q6ZPJ3.3|UBE2O_MOUSE (RecName: Full=(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O; AltName: Full=E2/E3 hybrid ubiquitin-protein ligase UBE2O; AltName: Full=Ubiquitin carrier protein O; AltName: Full=Ubiquitin-conjugating enzyme E2 O; AltName: Full=Ubiquitin-conjugating enzyme E2 of 230 kDa; Short=Ubiquitin-conjugating enzyme E2-230K; AltName: Full=Ubiquitin-protein ligase O)

HSP 1 Score: 268.855 bits (686), Expect = 5.358e-73
Identity = 134/265 (50.57%), Postives = 177/265 (66.79%), Query Frame = 0
Query:  807 EHKIEAPQVEKVVVS--PPEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISS 1069
            E K E P+V+  V +  P E  +   +    P     + K   F + E AP  H+FK   F+P + K F   V+KE  LL++SLP GI++K +EDR+DLFSA+IKGP+ TPY DG++ FD QLP  YP  PP   Y+S CS RLNPNLY+ GKVCVSLLGTW GKGTE WT  S+LLQ+L+SIQGLILV EPY+NEAG++S +  + G ENS  YNEM +I++VQSMT+L+  PP +F+ EI +H +  G + V R   WL+  +
Sbjct:  883 ERKEEKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLVNRIESWLETHA 1147          

HSP 2 Score: 130.954 bits (328), Expect = 1.718e-29
Identity = 81/205 (39.51%), Postives = 112/205 (54.63%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVV--RASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEE-SVYDLRDHPDFNYRPGSVVIKVANFDN----EESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKV------GEYDSDEG-------ELWDDDSD 583
             +PG+ V VE + T     V+WQDGS+E  I S++L+P+HHLD+ EF  G+FVV  R         YGVVQ  D+  R   +KWF     G D     +GE E+ SVYD+ DHPDF +R   +VI++ N ++    +E E  + G+V  V  + KV V WA+   T   PQ LY +       +YDS EG       + W+DDSD
Sbjct:  533 FKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDD--VELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSV-GQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSSSGASSDEWEDDSD 734          

HSP 3 Score: 98.9821 bits (245), Expect = 1.324e-19
Identity = 79/248 (31.85%), Postives = 125/248 (50.40%), Query Frame = 0
Query:   54 LKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLF--KRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIA--PDIIQGEDLKRTKILGYFFPCTLQIGDRFFY 297
            L+ G V V WYP+  ++    + LKL +RS++P DVVR M S    SQ G   ++ +  +  +  +N  +IY +NS+DL+ I  F+    +    W+G +  +K  + L   +G +RC ++    +   DV        LF      +YPG +L GP + F   +W S    +K ++S K  KF +V V + QV EL V W  ++     +D ++  P II  E+L R K LG F     Q+G+R  Y
Sbjct:  112 LRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRST--DSQCGTVIDVNIDCAVKLIGTN-CIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNG-ARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLS---GVKPVLSTK-SKF-RVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSIITQENLGRVKRLGCFDHAQRQLGERCLY 350          
BLAST of EMLSAG00000000488 vs. SwissProt
Match: gi|209572710|sp|Q9C0C9.3|UBE2O_HUMAN (RecName: Full=(E3-independent) E2 ubiquitin-conjugating enzyme; AltName: Full=E2/E3 hybrid ubiquitin-protein ligase UBE2O; AltName: Full=Ubiquitin carrier protein O; AltName: Full=Ubiquitin-conjugating enzyme E2 O; AltName: Full=Ubiquitin-conjugating enzyme E2 of 230 kDa; Short=Ubiquitin-conjugating enzyme E2-230K; AltName: Full=Ubiquitin-protein ligase O)

HSP 1 Score: 267.314 bits (682), Expect = 2.108e-72
Identity = 125/226 (55.31%), Postives = 162/226 (71.68%), Query Frame = 0
Query:  844 KNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISS 1069
            K   F + E AP  H+FK   F+P + K F   V+KE  LL++SLP GI++K +EDR+DLFSA+IKGP+ TPY DG++ FD QLP  YP  PP   Y+S CS RLNPNLY+ GKVCVSLLGTW GKGTE WT  S+LLQ+L+SIQGLILV EPY+NEAG++S +  + G ENS  YNEM +I++VQSMT+L+  PP +F+ EI +H +  G + V R   WL+  +
Sbjct:  925 KGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPEGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHA 1150          

HSP 2 Score: 130.954 bits (328), Expect = 1.797e-29
Identity = 81/205 (39.51%), Postives = 111/205 (54.15%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVV--RASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEE-SVYDLRDHPDFNYRPGSVVIKVANFDN----EESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKV------GEYDSDEGEL-------WDDDSD 583
             +PG+ V VE + T     V+WQDGS+E  I S++L+P+HHLD+ EF  G+FVV  R         YGVVQ  D+  R   +KWF     G D     +GE E+ SVYD+ DHPDF +R   +VI++ N ++    +E E  + G+V  V  + KV V WA+   T   PQ LY +       +YDS EG         W+DDSD
Sbjct:  538 FKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHIGRTCMVKWFKLRPSGDD--VELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSV-GQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSD 739          

HSP 3 Score: 98.5969 bits (244), Expect = 1.545e-19
Identity = 78/248 (31.45%), Postives = 125/248 (50.40%), Query Frame = 0
Query:   54 LKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLF--KRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIA--PDIIQGEDLKRTKILGYFFPCTLQIGDRFFY 297
            L+ G V V WYP+  ++    + LKL +RS++P DVVR M S    SQ G   ++ +  +  +  +N  +IY +NS+DL+ I  F+    +    W+G +  +K  + L   +G +RC ++    +   DV        LF      +YPG +L GP + F   +W S    +K ++S K  KF +V V + QV EL V W  ++     +D ++  P +I  E+L R K LG F     Q+G+R  Y
Sbjct:  117 LRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRST--DSQCGTVIDVNIDCAVKLIGTN-CIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNG-ARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLS---GVKPVLSTK-SKF-RVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLY 355          
BLAST of EMLSAG00000000488 vs. SwissProt
Match: gi|75315951|sp|Q9ZVX1.1|UBC23_ARATH (RecName: Full=Probable ubiquitin-conjugating enzyme E2 23; AltName: Full=E2 ubiquitin-conjugating enzyme 23; AltName: Full=Ubiquitin carrier protein 23)

HSP 1 Score: 201.06 bits (510), Expect = 2.114e-51
Identity = 97/201 (48.26%), Postives = 135/201 (67.16%), Query Frame = 0
Query:  855 PQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTP-NSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERG 1054
            P +H F     +    + + K V ++ K+L ++LP GI ++ YEDR+DL  A+I G   TPY DG+FFFDF LP DYP  PP+ +Y S    RLNPNLYEEGKVC+SLL TW+G+G E+W P +S++LQ+L+S+QGL+L  +PYFNEAGY+ Q  T  GE+NS  YNE   +   ++M  L+  PP  F++ I +H  +RG
Sbjct:  833 PLDHHFLGVDGQKTKERQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYYHSGGW-RLNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRG 1032          

HSP 2 Score: 75.485 bits (184), Expect = 2.026e-12
Identity = 111/495 (22.42%), Postives = 198/495 (40.00%), Query Frame = 0
Query:   54 LKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRC---VVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLRE-FEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIAPDIIQGEDLKRTKILGYFFPCTLQIGD----------------RFFYKIKEEDVILSKSKWLQREREILANEMDGESKPVDSIKLKEFDVKKEEAESKSLSVPNNSINKSNVSISVNKLRRRLKKRNKSVTAELYSNLPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVV-RASDLGGLQS----YGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVA 523
            L+  ++ V W    E    +N  + + +R  + GD V          Q G    + V  S  +   +  +  DI++++LK ++ F     V  GPW+G I  V  NV + F DG S C    V+   +      N +E          YYPG  +       F+ + W S   K   L           TV K     + V W   A  G +S    P+     +L    +L  F     Q+GD                +   K++  D    + + + R+ E + +E+ G+ +P          V  ++   +     N S++K  V       R++++K       ++     S   +++             + ++  V V WQDG++E   ++  L PI    D EF + ++VV + SD G   +     GVV++V+ + R A ++W +  +   +    +  E   SVY+L  HPD++Y  G VV++++
Sbjct:  116 LEGDQIRVLWMDNTEPVQDIND-VTVIDRGFLHGDYVASASE--PTGQVGVV--VDVNISVDLLAPDGSIHKDISTKNLKRVRDFAVGDYVVHGPWLGRIDDVLDNVTVLFDDG-SMCKVLRVEPLRLKPIPKNNLEED-----ANFPYYPGQRVKASSSSVFKNSRWLSGLWKPNRLEG---------TVTKVTAGSIFVYWIASAGFGPDSSVSPPEEQNPSNL---TLLSCFTHANWQVGDWCLLPSLNQSATIPLHKHVSKLRLYDSQADRQQKIGRDLEDVQDEVSGKVEPAGITAEALPKVTSDDPPQR-----NPSVSKEPVHEPWPLHRKKIRKLVIRKDKKVKKKEESFEQALL------------VVNSRTRVDVSWQDGTIECRREAITLIPIETPGDHEFVSEQYVVEKTSDDGDNTTEPRRAGVVKNVNAKDRTASVRWLNPLRRAEE-PREFEKEEIVSVYELEGHPDYDYCYGDVVVRLS 569          
BLAST of EMLSAG00000000488 vs. SwissProt
Match: gi|678021173|sp|Q9C918.2|UBC38_ARATH (RecName: Full=Putative ubiquitin-conjugating enzyme E2 38; AltName: Full=E2 ubiquitin-conjugating enzyme 38; AltName: Full=Ubiquitin carrier protein 38)

HSP 1 Score: 165.622 bits (418), Expect = 6.380e-44
Identity = 86/190 (45.26%), Postives = 117/190 (61.58%), Query Frame = 0
Query:  871 KNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPN-SNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVK 1059
            KN++K V+ E K+L+ +LP  I ++  E R+DL  A+I G   TPYHDG+FFFD Q P  YP  PP  +Y S    R+NPNLY  GKVC+SLLGTW G   + W P  S +LQ+L+SIQ L+L ++PYFNE GY   K T  GE  S +Y+E V +  +++M   +  PP  F++ +  H   R    VK
Sbjct:   53 KNWVKKVQDEWKILNQNLPETIFVRACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHS-GGLRINPNLYNCGKVCLSLLGTWHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGESKSKVYSENVFLLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVK 241          
BLAST of EMLSAG00000000488 vs. SwissProt
Match: gi|205828971|sp|Q8GY87.2|UBC26_ARATH (RecName: Full=Probable ubiquitin-conjugating enzyme E2 26; AltName: Full=E2 ubiquitin-conjugating enzyme 26; AltName: Full=Ubiquitin carrier protein 26)

HSP 1 Score: 169.474 bits (428), Expect = 3.056e-43
Identity = 88/190 (46.32%), Postives = 116/190 (61.05%), Query Frame = 0
Query:  871 KNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPN-SNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVK 1059
            KN++K V+ + K+L + LP  I ++  E R+DL  A+I G   TPYHDG+FFFD Q P  YP  PP   Y S    R+NPNLY  GKVC+SLLGTW+G   E W PN S +LQLL+SIQ LIL ++PYFNE GY     T  GE  S +Y+E V +  +++M   +  PP  F++ +  H   R    VK
Sbjct:  270 KNWVKKVQADWKILENDLPEAISVRACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHS-GGLRINPNLYNCGKVCLSLLGTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKSKVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVK 458          
BLAST of EMLSAG00000000488 vs. SwissProt
Match: gi|75311600|sp|Q9LUQ5.1|UBC25_ARATH (RecName: Full=Probable ubiquitin-conjugating enzyme E2 25; AltName: Full=E2 ubiquitin-conjugating enzyme 25; AltName: Full=Ubiquitin carrier protein 25)

HSP 1 Score: 170.629 bits (431), Expect = 4.311e-43
Identity = 86/181 (47.51%), Postives = 112/181 (61.88%), Query Frame = 0
Query:  871 KNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPN-SNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHI 1050
            K++ K ++ E ++L   LP  I ++ YE R+DL  A+I G   TPYHDG+FFFD   P  YP +PP   Y S    R+NPNLY  GKVC+SLLGTWSG   E W PN S +LQ+L+SIQGLIL ++PYFNE GYE    + HGE  S  Y+E   I  +++M   +  PP  F+D    H 
Sbjct:  331 KDWAKRIQDEWRILEKDLPEMIFVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHS-GGLRINPNLYNCGKVCLSLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGSAHGESTSKAYSENTFILSLKTMVYTMRRPPKYFEDFAYGHF 510          
BLAST of EMLSAG00000000488 vs. SwissProt
Match: gi|678019143|sp|F4HPP7.1|UBC39_ARATH (RecName: Full=Putative ubiquitin-conjugating enzyme E2 39; AltName: Full=E2 ubiquitin-conjugating enzyme 39; AltName: Full=Ubiquitin carrier protein 39)

HSP 1 Score: 157.532 bits (397), Expect = 3.166e-41
Identity = 82/163 (50.31%), Postives = 107/163 (65.64%), Query Frame = 0
Query:  870 PKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPN-SNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSM 1031
            PKN++K ++KE K+L  +LP  I ++  E RIDL  A+I G   TPYHDG+FFFD Q P  YP  PP   Y S    R+NPNLY+ GKVC+SL+ TW+GK  E W P  S +LQLL+SIQ LIL ++PY+NE GYE    T  GE  S  Y+E V +  +++M
Sbjct:   55 PKNWVKDIQKEWKILDKNLPETIFVRACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHS-GGLRINPNLYKCGKVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEKSMGTPLGESYSKDYSENVFVFSLKTM 216          
BLAST of EMLSAG00000000488 vs. SwissProt
Match: gi|75331117|sp|Q8VY10.1|UBC24_ARATH (RecName: Full=Probable ubiquitin-conjugating enzyme E2 24; AltName: Full=AtPHO2; AltName: Full=E2 ubiquitin-conjugating enzyme 24; AltName: Full=Ubiquitin carrier protein 24; AltName: Full=Ubiquitin-protein ligase 24)

HSP 1 Score: 166.007 bits (419), Expect = 1.216e-40
Identity = 89/197 (45.18%), Postives = 124/197 (62.94%), Query Frame = 0
Query:  871 KNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPN-SNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZ 1066
            K+++K V++E   L ++LP+ I ++  E+R+DL  A + G   TPYHDG+FFFD  LPP YP  PP   Y S    RLNPNLYE G+VC+SLL TWSG GTE+W    S++LQLLLS Q L+L ++PYFNEAGY+ Q     GE+NS  YNE   +   +SM  +L  PP  F+  + +H   R    +   + +++
Sbjct:  661 KSWVKKVQQEWSNLEANLPNTIYVRVCEERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHS-GGMRLNPNLYESGRVCLSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVSYNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYME 856          

HSP 2 Score: 75.485 bits (184), Expect = 1.687e-12
Identity = 53/181 (29.28%), Postives = 90/181 (49.72%), Query Frame = 0
Query:  411 RTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSY--VGESEESVYDLRDHPDFNYRPGSVVIKV---ANFDNEE------------SEGGLGGE-------VIEVYPNGKVTVWWANKKTTECWPQDLYKV 567
            +TK +V+V+WQDGS   G+DS +L P+  ++  +F+  +FVV        + +GVV+ V+ + +  K++W  T Q+  + +     V E   S Y+L +HPDF +    VV+K+     FD               +E    G        V+  + NG V V WAN  T++  P +++K+
Sbjct:  300 KTKMKVAVMWQDGSCSLGVDSQQLLPVGAVNAHDFWPEQFVVEKETCNS-KKWGVVKAVNAKEQTVKVQW--TIQVEKEATGCVDEVMEEIVSAYELLEHPDFGFCFSDVVVKLLPEGKFDPNADTIVATEAKHLLTESDYSGAYFLSSIGVVTGFKNGSVKVKWANGSTSKVAPCEIWKM 477          
BLAST of EMLSAG00000000488 vs. SwissProt
Match: gi|408360245|sp|Q11076.3|UBC17_CAEEL (RecName: Full=Probable ubiquitin-conjugating enzyme protein 17)

HSP 1 Score: 152.525 bits (384), Expect = 9.101e-37
Identity = 86/238 (36.13%), Postives = 137/238 (57.56%), Query Frame = 0
Query:  867 PVDP--KNFLKFVKKEAKLLSSSLP----SGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSD--RLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTR-LLISPPPIFKDEILEHINERGDKFVKRWREWLZ-ISSSY----HSNKKEISPLDFPLLPASK 1090
            P  P  ++  K + KE   ++++LP    + I +   E R+D+   +I GP DTPY +G+F FD   P  YP SPP C +++  S   R NPNLY +GK+C+S+LGTW G+  E W P  +L+Q+L+SIQGLI VK+PYFNE G+E  + T  G+E S  YN  +    +    R  L  P   FK+ I +H+  + +  +K+ + W++ +S+ +     S +K++   +    PA++
Sbjct:  395 PFSPHLRDRTKRIAKELASIANALPLNASNSIYVCYDEGRVDIIKVLISGPDDTPYANGLFEFDIFFPTGYPFSPPKCAFLTTGSGNVRFNPNLYNDGKICLSILGTWEGRPEEKWNPYCSLMQVLVSIQGLIFVKDPYFNEPGFERYQGTDRGDEYSRKYNLQIEHATLNYAIREQLKKPSEHFKEVIEKHLWLKREAILKQAQAWIDNVSNDFGDDKMSKRKDVFAFETGFNPATQ 632          
BLAST of EMLSAG00000000488 vs. SwissProt
Match: gi|380876899|sp|O88738.2|BIRC6_MOUSE (RecName: Full=Baculoviral IAP repeat-containing protein 6; AltName: Full=BIR repeat-containing ubiquitin-conjugating enzyme; Short=BRUCE; AltName: Full=RING-type E3 ubiquitin transferase BIRC6; AltName: Full=Ubiquitin-conjugating BIR domain enzyme apollon; Short=APOLLON)

HSP 1 Score: 147.132 bits (370), Expect = 3.245e-34
Identity = 76/183 (41.53%), Postives = 108/183 (59.02%), Query Frame = 0
Query:  898 EDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFY--ISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTP-NSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQ-SMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKK 1076
            E+R+D+   +I GP+DTPY +G F FD   P DYP SPP          S R NPNLY +GKVC+S+L TW G+  E W P  S+ LQ+L+S+Q LILV EPYFNE GYE  + T  G ++S  Y+  +    V+ +M   + +P P FK+ I +H   +  + + +  EW+     Y S+K+
Sbjct: 4628 EERLDIMKVLITGPADTPYANGCFEFDVYFPQDYPSSPPLVNLETTGGHSVRFNPNLYNDGKVCLSILNTWHGRPEEKWNPQTSSFLQVLVSVQSLILVAEPYFNEPGYERSRGTPSGTQSSREYDGNIRQATVKWAMLEQIRNPSPCFKEVIHKHFYLKRIELMAQCEEWIADIQQYSSDKR 4810          
BLAST of EMLSAG00000000488 vs. Select Arthropod Genomes
Match: gb|KFM57180.1| (Ubiquitin-conjugating enzyme E2 O, partial [Stegodyphus mimosarum])

HSP 1 Score: 704.131 bits (1816), Expect = 0.000e+0
Identity = 439/1232 (35.63%), Postives = 666/1232 (54.06%), Query Frame = 0
Query:   10 AQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRM---HLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQAC----VGNESDGIAPDIIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREILANEMDGESKPVDSIKLKEFDVKKEEAESKSLSVPNNSI-------------------------NKSNVSISVNKLRRRLKKRNKSVTAELYSNLPS----CYSSVIPKIRP---GEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGEL----------WDDDSD--FEGEEKDEVEEDSWVMLTKL------VGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTS--GKLNKVAGNKLAVQDQISRLFNS---TSEFQNINAKENSLPSND-----------EQDIFYE----------------------------------------NTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKI-EAPQVEKVVVSPPEEVLSPVELQSLPLEA--VLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTP-NSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYH---------------------SNKKEISPLDFPLLPASKGFCVSFAKSLDEF 1103
             + F ED VY ++KK  +  G V+ + E ++ DD E +  D      +K+G + V+W+P   E+V     +KL +RSLMPGDVVR++   GK +Q GYCR   V+    +     +V+  ++S+DL P++ F+ + AV    WVG+++ VK  + L   DG SRC++ D      DD+ D+ ++ + FK+  +YPG  L GP   F+  +W   +  + ++++  P K V+  V   +V  L+V W C+A     V N+S       + G+DLK  K++  F PCTLQIGDR +  +K+   ++S ++W  R    L   +D  + P+    LK+ + + +E ++++  V N ++                         + +  SI  N+  ++  K+      ++     S        V+ +  P   GE +V EAL T+ +  V+WQDG++E  I S+ LYP+HHLD  EFF G++VV   D    + YGVV+ VD+  R A I W  TY  G DP P  + E E SVYD++DHPDF YRPGS VI+V N + + + G   G+V++V+ +GKV V+W + +T+ C+PQ+LY+VGEYDSD+             W+ +S+    G E++  E+D       L      +   + EK++E +  EK     +    EK+R  + +LEE F++    QT  ++ +L E+YK C VL++ +GT+YF      ++++R    G++N +      + DQI RLF+S   TS+     +K+  +   D           E DIF E                                        N C+++C++ K +L   ++    ++G  +P+           + D LL+         + +LPK+       +     K + +  E + S   L +L   +    +   G F + ES P  H +K+S+F+P +P  F + V+KE  LL SSLP GI++K +EDRIDL+S MIKGP  TPY DG+FFFDFQ P DYPK PP C YIS+CSDRLNPNLYE GKVCVSLLGTW GKGTEIW P  SNLLQ+++SIQGLILV EPY+NEAGYE QK T+ G ENS MYNEMVV+K+VQ+MT+LL++PP +FK EI+EH   +  K ++R  +WL +S S++                     S+ +EI   +FPL+PASKGFC++  KSL +F
Sbjct:    5 CKFFDEDAVYKINKKKFVDVGLVLENSEFISSDDEESDGPDYKDWERMKKGHIRVAWHPNGVEEVLPEKKVKLCDRSLMPGDVVRKIVK-GKDTQLGYCRYTVVKADVQV-VGTKQVVVGVDSKDLIPMEEFVADVAVFKDSWVGMVKTVKTELTLRCCDG-SRCIMSDVDAFDMDDILDRRERASEFKKYSFYPGQQLWGPTFCFQKVKWLVLNKPMSDIINC-PNKSVRAVVEDVKVVSLAVQWVCKAYSEENVRNQSLEQPKYHVTGDDLKSIKMMNIFEPCTLQIGDRNYLIMKDSYEVISLNEWKNRMTSRL--NVDPHNSPIKRRNLKKSENENKENDAETDIVCNENMDDSSDFEDIDDDDIGKSSDTASVTSHSTASSIETNQGSQKKSKKGPHYATKMLKKKKSVRRRAMRHVMKETLPLTSGEKLVTEALITETKAHVVWQDGTVEHNILSTSLYPVHHLDSHEFFPGDYVVDGKDSRPYE-YGVVKQVDHSGRTALITWLKTYHAGGDPHPEVLSEQEVSVYDIKDHPDFKYRPGSCVIRVVNSE-DSNPGSTAGQVVDVHSSGKVEVYWVSGETSLCYPQELYRVGEYDSDDLWADDDSFSDEDSWETESEKSLVGTEENTTEKDVDGTARDLEKVSFKLEEKDDEKSTEDM-HEK-----LLANIEKLRAGMARLEEVFTQNPTLQTCNVMRRLLELYKHCKVLDQLLGTTYFHERHLQSLVERLKHRGRVNSIQ----HMADQIFRLFSSNDPTSDVNESQSKDEVISDFDKINNVSDSPKVEIDIFIESPCDAPLPTINSVNCNEDMSNRKPLLSPVSPTSGNGSEPRNLCQQLCAILKSRLLKAHEDAELRFG--MPR----------SETDALLAAE-------NELLPKDTSSKDTSQGESANKNLTNENEGLESKESLNNLEDHSSEFNSAGQGIFSMLESVPDSHKYKLSIFQPFEPCAFFRIVRKEMNLLKSSLPEGIIVKSFEDRIDLYSVMIKGPKRTPYEDGLFFFDFQFPADYPKVPPLCHYISYCSDRLNPNLYEGGKVCVSLLGTWGGKGTEIWNPETSNLLQVIVSIQGLILVSEPYYNEAGYERQKGTQQGRENSRMYNEMVVLKLVQAMTKLLVNPPEVFKSEIVEHFKTQALKLIQRLEKWLAVSESWNSEHPSSPTSPTTFKELHGNCDSSAQEIPLPEFPLIPASKGFCLTLRKSLADF 1199          
BLAST of EMLSAG00000000488 vs. Select Arthropod Genomes
Match: gb|EFA05319.1| (hypothetical protein TcasGA2_TC015477 [Tribolium castaneum])

HSP 1 Score: 483.797 bits (1244), Expect = 2.723e-152
Identity = 365/1141 (31.99%), Postives = 569/1141 (49.87%), Query Frame = 0
Query:    9 IAQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDG----ISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIAPDIIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQRER-EILANEM-DGESKPVDSIKLKEFDVKKEEAESKSLSVPNNSINKSNVSI-SVNKLRRRLKKRNKSVTAELYSNLPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDD-DSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEK--FANIIDRTSGK-----------------LNKVAGNKLAVQDQISRL--FNSTSEFQNINAKE---NSLP----SNDEQDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKIEAPQVEKVVVSP--PEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPLDFPLLPASKGFCVSFAKSLDEFDLAHSKYL 1111
            + + +  D VY  D++  IVFG V    E  + D++         L++G++LV+W     +++   S + L  R + PGD+VRR++  GK +Q GYC++ K   +  I   +D+VI  +    L  ++ F    AVCLG   G I+ +   + +    G    + R +  +     F  +N    +         YPG  +          +W     K K +M K  +   K T+   ++  + V W         ++      ++ +++KR K+L Y     L +GDR+F K+K  D++L K  W++++  + L +++   ++  + +   + FD   +    +       S NK  +SI  ++K+++R K          Y NL         K   G IV V+     A V+V+WQDG+ E+ I ++ELY     DD EFF GE+VV  S+      YGVVQ V++Q R A+IKWF +  + ++  P  +  +E SVYDL+ H  F +RPGSVV      +++       G+VI+  P G V V W   K   CWPQ++  + E  S++ E  D  DSD      D++ ED++   T+ V S + + T E  +     RV      + IR  +  L E F   +  Q    ++ L  IY +   L+K +GTS+F+ E   F  ++  T  K                 L+     K A +D I ++    S    Q    KE    S P    S++  +   EN C  +  + K+++  +Y  I+ + G                      ++T LT    +P+ P            ++  +++P  P +V  P+EL  +P       KN  F I E AP  H +  S F P D + FL  ++KE KLL  SLP+G+ ++ Y++R+DL S MI+GP+ TPY DG+F FD QL PDYPK+PP   YIS+ ++ LNPNLY EGKVCVSLLGTW G+G+E+W PNS LLQL++SIQGLILV +PYFNEAGYE Q  T+ G ENS  YNE V++K+VQSMT LL + P +F++E+L H   +G+   +R  ++         +++ + P +FPLLP SKGF +S   +L+ F  A  K L
Sbjct:    6 VKEFYAHDLVYKFDERKRIVFGVVSESYESSDSDET-------CALQKGQILVTWSNPTRKRICRQSKVYLLCRCIAPGDIVRRLED-GKETQRGYCKKTKQFATVQI-VESDKVIEHVPDSRLCFVKPFQVGDAVCLGDKFGRIQSIDEMIRMQSKCGSIVEVRRSINYNIRDYWFHSINGAYHEAE-------YPGQTVFCTPMHLRDPKWIK---KSKSMMRKMEIG-QKFTIQSIEICLIEVWWYSPNGHTEYAE------LKDDEIKRLKVLPY--SENLVMGDRYFLKLKPTDILLKKRDWIKKKSLQSLPHKVPKSDNSTLTNTCAEPFDADDDWCTEED----EESDNKGEISIYGLHKIKQRKKT---------YPNLN--------KFSSGSIVAVQVFCADARVTVVWQDGTEEKDIPTTELYYSVSQDDHEFFPGEWVVSNSNENS-DKYGVVQRVNFQERTAEIKWFSS--VDANQEPQELATNESSVYDLKKHVKFVFRPGSVVKAKPTVEDKM------GKVIDSCPQGYVIVQWLGGKRENCWPQNIELILE--SEDFEFPDAFDSD------DDLSEDAYSWETESVESFDGDVTDEINLQNMAARV------DFIRNRISYLREAFKHYNITQNFHFVKDLLCIYDNSGYLDKYLGTSFFSTESKHFQALLLETKKKAKSLGIELRGRMFSRENLSCPTKMKNAEKDNIQKMIKLESKVNVQIEMCKECSGESCPTTPDSSEPAEKPQENLCVELLVMLKMRMDLIYAEIISRIGGR-------------------QALTVLTNSSENPLKPSPST-----TTALQNALLTPETPPKVAKPIEL--IP-------KNEAFSILEEAPDTHRYFSSKFVPKDLQKFLVALQKEYKLLKDSLPAGVWVRTYDNRMDLLSVMIRGPAKTPYEDGLFLFDIQLSPDYPKNPPGVHYISYITEPLNPNLYVEGKVCVSLLGTWMGRGSEMWGPNSTLLQLIVSIQGLILVAQPYFNEAGYERQTHTQQGCENSRTYNEFVILKLVQSMTELLNAAPKVFQNEVLAHFQAKGEAMCERLMKY--------CDEEPLVP-EFPLLPVSKGFKLSLGSALNGFRQALKKAL 1032          
BLAST of EMLSAG00000000488 vs. Select Arthropod Genomes
Match: EGK96947.1 (AGAP003090-PB [Anopheles gambiae str. PEST])

HSP 1 Score: 388.267 bits (996), Expect = 4.061e-114
Identity = 267/803 (33.25%), Postives = 402/803 (50.06%), Query Frame = 0
Query:   11 QLFHEDEVYHLD-KKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDG--ISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIA--------PDIIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQ----REREILANEMDGESKP-----------------------------------------------VDSIKLKEFDVKKEEAESKSLSVPNNSINKSNVSISVNKLRRRLKKRNKSVTAELYSN-LPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRA----SDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFN-----STSEFQNINAKENSLPSNDEQDIFYENT 741
            Q F+EDE++ L  K G++ FG V+      N+D+S++E  +    K GE+   W+P   E++     + L +R+LMPGDVVRRM   G+ +Q GYC EI VR    I      V+ ++ S+ L+P+ S   +SAVCL  WVG  + +   + L    G  +  C   D  +  F D N + ++   F    +YPG  L G + E    +W + S+++K L  K  M   K  V   +++ +SV+WQC+    +    ++        P+ I GEDLKR K L  F  C LQI D+ + K+++ D+I SK  W +    + RE L  E  G++KP                                               VDS +  E D + E ++  + S    S      S  + K  R+LK+R   + AE   +  P+           G+ VV EAL   +  +V+WQDG++E  I S+EL PIHHLDD EFF G+FV+      +     + YGV+Q VD+  R A++KWF TY   S+P PSY GESE SVYDL+DHPDF YRPG++VI+VANF  EE      G+V++ YPNG V VWW +   + CWPQD+++VG+YDS E   W   +D + E        SW    +    G++E  S S V  K P++      E+ R ++ +LEE F+     Q   +++KL  +YK C  L++ + TS+F    F  +++R     N+    ++  QDQ +RLFN     S +        E+  PS  ++ +F  +T
Sbjct:  121 QYFYEDEIFCLTPKNGHVKFGLVLD-----NYDESDEETTEEDAPKRGEIRACWHPDGREEIIKQRKVGLADRTLMPGDVVRRMVP-GRDTQRGYCHEITVRADLRI-IGTKYVVRNVASDRLRPLLSMPKDSAVCLDSWVGSTKHINEKLVLKSSCGSLVEVCPASD--LGMFRDHNMRFRR-GFFAEPLFYPGQQLVGLMSELANGKWLTTSNEMK-LSRKNYMLERKFIVQSVELQGVSVHWQCKVSSEDLEQKLSEGGSLQQPPEYITGEDLKRLKKLNLFESCMLQINDKNYLKLEDTDIIGSKQAWRKDMTSKYREQLLQESKGKAKPTSKAISGGEEEKKGGKGTKSGSAGSKLSARSAGKENGAQKVSTKLTSVDSDEGWETDEEGEHSDGAASSCSATSKTSPRKSPLLAKRVRKLKRRQ--LAAEFAGDRFPAA----------GDEVVTEALVVYSTATVVWQDGTIEPNIPSTELRPIHHLDDHEFFPGDFVLAGNTDPAKNPSFRDYGVIQDVDHHGRTARVKWFSTYTCTSEPQPSYNGESEVSVYDLKDHPDFQYRPGTIVIRVANFTGEEG-ASTAGQVLDNYPNGMVKVWWVDGFVSMCWPQDIFEVGQYDS-ENNFWGSVADSDAE--------SWETEAESCHFGDAEPGSPSSVVLK-PQLTAN--LERARVAIARLEELFNINPHLQNQEIMKKLMLVYKKCRFLDRLMNTSFFHERHFMGLVERVRKSNNQTTVERM--QDQKNRLFNQSASGSKTSTPTAGTSESVRPSLQQRALFSPST 885          

HSP 2 Score: 305.834 bits (782), Expect = 2.203e-85
Identity = 160/405 (39.51%), Postives = 233/405 (57.53%), Query Frame = 0
Query:  734 QDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPV-LPKEEGEHKIEAPQVEKVVVSPPEEVLS---PVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISS-----------------------------------------SYHSNKKEISPLDFPLLPASKGFC 1093
            + +  E    R+CSL K +L  V + I E+YG   P   I+  ++          +   T   + P   P   G   I   + + +  S P    +   P    S+P E        +F+I ESAP  H F +++F+  + ++F K V++E  LL ++LP G+ ++ +EDR+DL S MI+GP  TPY DG+F FD QL  DYP++PP C YIS+C+DRLNPNLYE+GKVCVSLLGTWSG+GTE+W P S LLQ+++SIQGLILV EPYFNEAGYE  + ++ G+ENS MYNEMV++K+VQSMT+L+ +PP +F+++IL H +  G +   R + W+++S+                                         S  ++ + I   +FPL+PAS+GFC
Sbjct:  967 EQLARECVSARLCSLIKGQLIKVLEEINERYGLEDPLKEIISVVE----------LKTPTLASMPPSWTPSPVGTPDIPGSEPDPLASSSPAVAGADAVPSSPSSVPTE--------SFQIIESAPTSHKFHLTIFQTSNAQSFYKAVQREHWLLRTALPPGVWVRTFEDRLDLLSVMIEGPKKTPYEDGLFLFDIQLGLDYPRAPPLCHYISYCTDRLNPNLYEDGKVCVSLLGTWSGRGTEVWGPTSTLLQVIVSIQGLILVAEPYFNEAGYEKLRGSQQGKENSRMYNEMVLLKLVQSMTKLMSNPPEVFREQILTHFHACGQRMYSRLKTWMELSNDCNRQPSASDQTGTAAIDVSAAATSILASSEQEASSATVDYSSEASARAIPQPEFPLIPASRGFC 1353          
BLAST of EMLSAG00000000488 vs. Select Arthropod Genomes
Match: EAA08452.5 (AGAP003090-PA [Anopheles gambiae str. PEST])

HSP 1 Score: 388.267 bits (996), Expect = 4.061e-114
Identity = 267/803 (33.25%), Postives = 402/803 (50.06%), Query Frame = 0
Query:   11 QLFHEDEVYHLD-KKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDG--ISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIA--------PDIIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQ----REREILANEMDGESKP-----------------------------------------------VDSIKLKEFDVKKEEAESKSLSVPNNSINKSNVSISVNKLRRRLKKRNKSVTAELYSN-LPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRA----SDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFN-----STSEFQNINAKENSLPSNDEQDIFYENT 741
            Q F+EDE++ L  K G++ FG V+      N+D+S++E  +    K GE+   W+P   E++     + L +R+LMPGDVVRRM   G+ +Q GYC EI VR    I      V+ ++ S+ L+P+ S   +SAVCL  WVG  + +   + L    G  +  C   D  +  F D N + ++   F    +YPG  L G + E    +W + S+++K L  K  M   K  V   +++ +SV+WQC+    +    ++        P+ I GEDLKR K L  F  C LQI D+ + K+++ D+I SK  W +    + RE L  E  G++KP                                               VDS +  E D + E ++  + S    S      S  + K  R+LK+R   + AE   +  P+           G+ VV EAL   +  +V+WQDG++E  I S+EL PIHHLDD EFF G+FV+      +     + YGV+Q VD+  R A++KWF TY   S+P PSY GESE SVYDL+DHPDF YRPG++VI+VANF  EE      G+V++ YPNG V VWW +   + CWPQD+++VG+YDS E   W   +D + E        SW    +    G++E  S S V  K P++      E+ R ++ +LEE F+     Q   +++KL  +YK C  L++ + TS+F    F  +++R     N+    ++  QDQ +RLFN     S +        E+  PS  ++ +F  +T
Sbjct:  121 QYFYEDEIFCLTPKNGHVKFGLVLD-----NYDESDEETTEEDAPKRGEIRACWHPDGREEIIKQRKVGLADRTLMPGDVVRRMVP-GRDTQRGYCHEITVRADLRI-IGTKYVVRNVASDRLRPLLSMPKDSAVCLDSWVGSTKHINEKLVLKSSCGSLVEVCPASD--LGMFRDHNMRFRR-GFFAEPLFYPGQQLVGLMSELANGKWLTTSNEMK-LSRKNYMLERKFIVQSVELQGVSVHWQCKVSSEDLEQKLSEGGSLQQPPEYITGEDLKRLKKLNLFESCMLQINDKNYLKLEDTDIIGSKQAWRKDMTSKYREQLLQESKGKAKPTSKAISGGEEEKKGGKGTKSGSAGSKLSARSAGKENGAQKVSTKLTSVDSDEGWETDEEGEHSDGAASSCSATSKTSPRKSPLLAKRVRKLKRRQ--LAAEFAGDRFPAA----------GDEVVTEALVVYSTATVVWQDGTIEPNIPSTELRPIHHLDDHEFFPGDFVLAGNTDPAKNPSFRDYGVIQDVDHHGRTARVKWFSTYTCTSEPQPSYNGESEVSVYDLKDHPDFQYRPGTIVIRVANFTGEEG-ASTAGQVLDNYPNGMVKVWWVDGFVSMCWPQDIFEVGQYDS-ENNFWGSVADSDAE--------SWETEAESCHFGDAEPGSPSSVVLK-PQLTAN--LERARVAIARLEELFNINPHLQNQEIMKKLMLVYKKCRFLDRLMNTSFFHERHFMGLVERVRKSNNQTTVERM--QDQKNRLFNQSASGSKTSTPTAGTSESVRPSLQQRALFSPST 885          

HSP 2 Score: 305.834 bits (782), Expect = 2.203e-85
Identity = 160/405 (39.51%), Postives = 233/405 (57.53%), Query Frame = 0
Query:  734 QDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPV-LPKEEGEHKIEAPQVEKVVVSPPEEVLS---PVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISS-----------------------------------------SYHSNKKEISPLDFPLLPASKGFC 1093
            + +  E    R+CSL K +L  V + I E+YG   P   I+  ++          +   T   + P   P   G   I   + + +  S P    +   P    S+P E        +F+I ESAP  H F +++F+  + ++F K V++E  LL ++LP G+ ++ +EDR+DL S MI+GP  TPY DG+F FD QL  DYP++PP C YIS+C+DRLNPNLYE+GKVCVSLLGTWSG+GTE+W P S LLQ+++SIQGLILV EPYFNEAGYE  + ++ G+ENS MYNEMV++K+VQSMT+L+ +PP +F+++IL H +  G +   R + W+++S+                                         S  ++ + I   +FPL+PAS+GFC
Sbjct:  967 EQLARECVSARLCSLIKGQLIKVLEEINERYGLEDPLKEIISVVE----------LKTPTLASMPPSWTPSPVGTPDIPGSEPDPLASSSPAVAGADAVPSSPSSVPTE--------SFQIIESAPTSHKFHLTIFQTSNAQSFYKAVQREHWLLRTALPPGVWVRTFEDRLDLLSVMIEGPKKTPYEDGLFLFDIQLGLDYPRAPPLCHYISYCTDRLNPNLYEDGKVCVSLLGTWSGRGTEVWGPTSTLLQVIVSIQGLILVAEPYFNEAGYEKLRGSQQGKENSRMYNEMVLLKLVQSMTKLMSNPPEVFREQILTHFHACGQRMYSRLKTWMELSNDCNRQPSASDQTGTAAIDVSAAATSILASSEQEASSATVDYSSEASARAIPQPEFPLIPASRGFC 1353          
BLAST of EMLSAG00000000488 vs. Select Arthropod Genomes
Match: XP_006557525.1 (PREDICTED: (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O [Apis mellifera])

HSP 1 Score: 346.28 bits (887), Expect = 1.335e-99
Identity = 156/273 (57.14%), Postives = 202/273 (73.99%), Query Frame = 0
Query:  848 FKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPL-----------------DFPLLPASKGFCVSFAKSLDEF 1103
            F IEE+AP  H FK+++F+P DP NF + V KE KLL SSLP GI +KG+EDRIDL+S M +GP  TPY DG+F FDFQL  DYP +PP C YIS+C+DRLNPNLYE+GKVCVSLLGTWSG+GTE+WT +S LLQ+++SIQGLILV EPYFNEAG++ QK ++ G+ENS MYNEMVV+K+VQ+ T+LL  PPP+FKD I+EH      K ++R   W++IS   H+N+  +SP+                 +FPL+PAS+GFC++  K+L  F
Sbjct:  953 FSIEETAPDSHKFKLTMFQPTDPTNFFRTVSKELKLLKSSLPPGIWVKGFEDRIDLYSVMFRGPEKTPYEDGLFLFDFQLSADYPAAPPLCHYISYCNDRLNPNLYEDGKVCVSLLGTWSGRGTEVWTSSSTLLQVIVSIQGLILVPEPYFNEAGFDKQKGSQQGKENSRMYNEMVVLKLVQAQTKLLQHPPPVFKDIIIEHFKRHAKKLLQRLELWMEISEQ-HNNQHPLSPVTPTTFKQISDVDNKILPEFPLIPASRGFCITLRKTLATF 1224          

HSP 2 Score: 283.108 bits (723), Expect = 6.953e-78
Identity = 149/349 (42.69%), Postives = 216/349 (61.89%), Query Frame = 0
Query:  402 GEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDD---DSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFNSTSEFQNINAKENSLPSNDEQDIFYENTCRRICS 747
            G  +VVE L T  + +V+WQDGS+E GI S++LYPIHHLDDKEFF G+FVV   +   +  YGVVQ VD+Q R AKIKWF TY     P P+ + E E SVYDL+DHPDF YRPG++VI++ANF+ E++ G   G+V++ YP G+V VWW +   + CWPQDLYKVGEYDSDEGELWDD   D+ +E E      ED ++         +++ T ++ +    P++      EK R ++ +LEE F++    QT+ ++ KL E+YKDC  ++K +GTS+F    F  +++R   +       ++A  DQ++RLF   S+    N  +NS  +++       N C +I +
Sbjct:  448 GTKIVVETLSTSTKANVVWQDGSVEFGIPSTQLYPIHHLDDKEFFPGDFVVDQKEESRM--YGVVQSVDHQGRTAKIKWFKTYTSTQSPQPTLLEEREVSVYDLKDHPDFQYRPGTLVIRIANFEGEDA-GCTAGQVLDNYPEGRVKVWWVDGHVSMCWPQDLYKVGEYDSDEGELWDDVSSDASWETE-----LEDCFI--------ADTDGTEQTELENIKPKLAAH--IEKARIAMSRLEEIFTQNPSLQTTEVMRKLLEVYKDCRYMDKLMGTSFFHECHFQGLLERVRERGRANVAQRMA--DQVTRLFTHKSDGSEENIDKNSTENSNNIVQSGSNLCEKIIT 776          

HSP 3 Score: 208.764 bits (530), Expect = 6.571e-54
Identity = 118/316 (37.34%), Postives = 186/316 (58.86%), Query Frame = 0
Query:   10 AQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVV---DDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACV--GNESDGIAPD-IIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREIL 319
             Q F+ED VY +DKKGN+VFG V+ + +  +  +   + E+    K+GE+ V W+P   E++  +  + L +R+LMPGDVVRRM   GK +Q GYCR+I++ T+        +V+ +I SEDL P++ F T+ AVC+  WVG I+     + L   DG SRCV+   D  V+   ++  D +         ++YPG  L GP+   E A+W +C+ ++K     KP K  KV V K +   + V+WQC+A    G  SD   P  ++QGE+LK+ K+L  F P T+Q+GDR FY IK ++ ++++ +W +++R+I 
Sbjct:    9 CQYFYEDVVYRVDKKGNVVFGIVMENDDQ-DLSEESSDSEESPKRKKGEIRVVWHPSGVEELVSSKKVHLADRTLMPGDVVRRMIK-GKDTQRGYCRDIEL-TACVQVIGTKQVLTNIRSEDLVPLEEFATDIAVCMDSWVGGIKMANFKLWLTTPDG-SRCVINETDSRVLGQLEERRDNDNDFP--HSSEFYPGQSLWGPVHCLEDAQWITCTKEMKAKRKSKPQKLTKVIVEKVETDWVGVHWQCRAYSKDGAWSDQAQPKFVVQGENLKKLKLLNVFEPSTVQVGDRNFYVIKGDENVITREQWRKQQRDIF 318          
BLAST of EMLSAG00000000488 vs. Select Arthropod Genomes
Match: XP_006557524.1 (PREDICTED: (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O [Apis mellifera])

HSP 1 Score: 346.28 bits (887), Expect = 1.335e-99
Identity = 156/273 (57.14%), Postives = 202/273 (73.99%), Query Frame = 0
Query:  848 FKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPL-----------------DFPLLPASKGFCVSFAKSLDEF 1103
            F IEE+AP  H FK+++F+P DP NF + V KE KLL SSLP GI +KG+EDRIDL+S M +GP  TPY DG+F FDFQL  DYP +PP C YIS+C+DRLNPNLYE+GKVCVSLLGTWSG+GTE+WT +S LLQ+++SIQGLILV EPYFNEAG++ QK ++ G+ENS MYNEMVV+K+VQ+ T+LL  PPP+FKD I+EH      K ++R   W++IS   H+N+  +SP+                 +FPL+PAS+GFC++  K+L  F
Sbjct:  953 FSIEETAPDSHKFKLTMFQPTDPTNFFRTVSKELKLLKSSLPPGIWVKGFEDRIDLYSVMFRGPEKTPYEDGLFLFDFQLSADYPAAPPLCHYISYCNDRLNPNLYEDGKVCVSLLGTWSGRGTEVWTSSSTLLQVIVSIQGLILVPEPYFNEAGFDKQKGSQQGKENSRMYNEMVVLKLVQAQTKLLQHPPPVFKDIIIEHFKRHAKKLLQRLELWMEISEQ-HNNQHPLSPVTPTTFKQISDVDNKILPEFPLIPASRGFCITLRKTLATF 1224          

HSP 2 Score: 283.108 bits (723), Expect = 6.953e-78
Identity = 149/349 (42.69%), Postives = 216/349 (61.89%), Query Frame = 0
Query:  402 GEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDD---DSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFNSTSEFQNINAKENSLPSNDEQDIFYENTCRRICS 747
            G  +VVE L T  + +V+WQDGS+E GI S++LYPIHHLDDKEFF G+FVV   +   +  YGVVQ VD+Q R AKIKWF TY     P P+ + E E SVYDL+DHPDF YRPG++VI++ANF+ E++ G   G+V++ YP G+V VWW +   + CWPQDLYKVGEYDSDEGELWDD   D+ +E E      ED ++         +++ T ++ +    P++      EK R ++ +LEE F++    QT+ ++ KL E+YKDC  ++K +GTS+F    F  +++R   +       ++A  DQ++RLF   S+    N  +NS  +++       N C +I +
Sbjct:  448 GTKIVVETLSTSTKANVVWQDGSVEFGIPSTQLYPIHHLDDKEFFPGDFVVDQKEESRM--YGVVQSVDHQGRTAKIKWFKTYTSTQSPQPTLLEEREVSVYDLKDHPDFQYRPGTLVIRIANFEGEDA-GCTAGQVLDNYPEGRVKVWWVDGHVSMCWPQDLYKVGEYDSDEGELWDDVSSDASWETE-----LEDCFI--------ADTDGTEQTELENIKPKLAAH--IEKARIAMSRLEEIFTQNPSLQTTEVMRKLLEVYKDCRYMDKLMGTSFFHECHFQGLLERVRERGRANVAQRMA--DQVTRLFTHKSDGSEENIDKNSTENSNNIVQSGSNLCEKIIT 776          

HSP 3 Score: 208.764 bits (530), Expect = 6.571e-54
Identity = 118/316 (37.34%), Postives = 186/316 (58.86%), Query Frame = 0
Query:   10 AQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVV---DDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACV--GNESDGIAPD-IIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREIL 319
             Q F+ED VY +DKKGN+VFG V+ + +  +  +   + E+    K+GE+ V W+P   E++  +  + L +R+LMPGDVVRRM   GK +Q GYCR+I++ T+        +V+ +I SEDL P++ F T+ AVC+  WVG I+     + L   DG SRCV+   D  V+   ++  D +         ++YPG  L GP+   E A+W +C+ ++K     KP K  KV V K +   + V+WQC+A    G  SD   P  ++QGE+LK+ K+L  F P T+Q+GDR FY IK ++ ++++ +W +++R+I 
Sbjct:    9 CQYFYEDVVYRVDKKGNVVFGIVMENDDQ-DLSEESSDSEESPKRKKGEIRVVWHPSGVEELVSSKKVHLADRTLMPGDVVRRMIK-GKDTQRGYCRDIEL-TACVQVIGTKQVLTNIRSEDLVPLEEFATDIAVCMDSWVGGIKMANFKLWLTTPDG-SRCVINETDSRVLGQLEERRDNDNDFP--HSSEFYPGQSLWGPVHCLEDAQWITCTKEMKAKRKSKPQKLTKVIVEKVETDWVGVHWQCRAYSKDGAWSDQAQPKFVVQGENLKKLKLLNVFEPSTVQVGDRNFYVIKGDENVITREQWRKQQRDIF 318          
BLAST of EMLSAG00000000488 vs. Select Arthropod Genomes
Match: XP_001120701.2 (PREDICTED: (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O [Apis mellifera])

HSP 1 Score: 346.28 bits (887), Expect = 1.335e-99
Identity = 156/273 (57.14%), Postives = 202/273 (73.99%), Query Frame = 0
Query:  848 FKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPL-----------------DFPLLPASKGFCVSFAKSLDEF 1103
            F IEE+AP  H FK+++F+P DP NF + V KE KLL SSLP GI +KG+EDRIDL+S M +GP  TPY DG+F FDFQL  DYP +PP C YIS+C+DRLNPNLYE+GKVCVSLLGTWSG+GTE+WT +S LLQ+++SIQGLILV EPYFNEAG++ QK ++ G+ENS MYNEMVV+K+VQ+ T+LL  PPP+FKD I+EH      K ++R   W++IS   H+N+  +SP+                 +FPL+PAS+GFC++  K+L  F
Sbjct:  953 FSIEETAPDSHKFKLTMFQPTDPTNFFRTVSKELKLLKSSLPPGIWVKGFEDRIDLYSVMFRGPEKTPYEDGLFLFDFQLSADYPAAPPLCHYISYCNDRLNPNLYEDGKVCVSLLGTWSGRGTEVWTSSSTLLQVIVSIQGLILVPEPYFNEAGFDKQKGSQQGKENSRMYNEMVVLKLVQAQTKLLQHPPPVFKDIIIEHFKRHAKKLLQRLELWMEISEQ-HNNQHPLSPVTPTTFKQISDVDNKILPEFPLIPASRGFCITLRKTLATF 1224          

HSP 2 Score: 283.108 bits (723), Expect = 6.953e-78
Identity = 149/349 (42.69%), Postives = 216/349 (61.89%), Query Frame = 0
Query:  402 GEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDD---DSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFNSTSEFQNINAKENSLPSNDEQDIFYENTCRRICS 747
            G  +VVE L T  + +V+WQDGS+E GI S++LYPIHHLDDKEFF G+FVV   +   +  YGVVQ VD+Q R AKIKWF TY     P P+ + E E SVYDL+DHPDF YRPG++VI++ANF+ E++ G   G+V++ YP G+V VWW +   + CWPQDLYKVGEYDSDEGELWDD   D+ +E E      ED ++         +++ T ++ +    P++      EK R ++ +LEE F++    QT+ ++ KL E+YKDC  ++K +GTS+F    F  +++R   +       ++A  DQ++RLF   S+    N  +NS  +++       N C +I +
Sbjct:  448 GTKIVVETLSTSTKANVVWQDGSVEFGIPSTQLYPIHHLDDKEFFPGDFVVDQKEESRM--YGVVQSVDHQGRTAKIKWFKTYTSTQSPQPTLLEEREVSVYDLKDHPDFQYRPGTLVIRIANFEGEDA-GCTAGQVLDNYPEGRVKVWWVDGHVSMCWPQDLYKVGEYDSDEGELWDDVSSDASWETE-----LEDCFI--------ADTDGTEQTELENIKPKLAAH--IEKARIAMSRLEEIFTQNPSLQTTEVMRKLLEVYKDCRYMDKLMGTSFFHECHFQGLLERVRERGRANVAQRMA--DQVTRLFTHKSDGSEENIDKNSTENSNNIVQSGSNLCEKIIT 776          

HSP 3 Score: 208.764 bits (530), Expect = 6.571e-54
Identity = 118/316 (37.34%), Postives = 186/316 (58.86%), Query Frame = 0
Query:   10 AQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVV---DDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACV--GNESDGIAPD-IIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREIL 319
             Q F+ED VY +DKKGN+VFG V+ + +  +  +   + E+    K+GE+ V W+P   E++  +  + L +R+LMPGDVVRRM   GK +Q GYCR+I++ T+        +V+ +I SEDL P++ F T+ AVC+  WVG I+     + L   DG SRCV+   D  V+   ++  D +         ++YPG  L GP+   E A+W +C+ ++K     KP K  KV V K +   + V+WQC+A    G  SD   P  ++QGE+LK+ K+L  F P T+Q+GDR FY IK ++ ++++ +W +++R+I 
Sbjct:    9 CQYFYEDVVYRVDKKGNVVFGIVMENDDQ-DLSEESSDSEESPKRKKGEIRVVWHPSGVEELVSSKKVHLADRTLMPGDVVRRMIK-GKDTQRGYCRDIEL-TACVQVIGTKQVLTNIRSEDLVPLEEFATDIAVCMDSWVGGIKMANFKLWLTTPDG-SRCVINETDSRVLGQLEERRDNDNDFP--HSSEFYPGQSLWGPVHCLEDAQWITCTKEMKAKRKSKPQKLTKVIVEKVETDWVGVHWQCRAYSKDGAWSDQAQPKFVVQGENLKKLKLLNVFEPSTVQVGDRNFYVIKGDENVITREQWRKQQRDIF 318          
BLAST of EMLSAG00000000488 vs. Select Arthropod Genomes
Match: EEB14790.1 (ubiquitin-conjugating enzyme E2-230k, putative [Pediculus humanus corporis])

HSP 1 Score: 338.191 bits (866), Expect = 8.923e-97
Identity = 181/430 (42.09%), Postives = 254/430 (59.07%), Query Frame = 0
Query:  712 FNSTSEFQNINAKENSLPSNDEQDIFYENTCRRICSLFKLKLAYVYDVIVEKY-GTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKIEAPQVEKVVVSPPEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZIS--------------SSYHSN----------------KKEISPL-------DFPLLPASKGFCVSFAKSLDEF 1103
            F++   F  +  +++S    +  D      C ++C+L K +L   +  +  ++ G   P    L E+ ++   ++ L     T DD +     ++ E        EK  +S P                 L   N  F I  +AP  H +K+++ +  DPK F K VKKE  LL +SLP GI +KG+EDR DL+S MI+GP  TP+ DG+F FDFQLP DYP++PP C Y+S+CSDRLNPNLYE+GKVC+SLLGTWSGKGTE+WT NSNLLQ+++SIQGLILV EPYFNEAGYE QK ++ G+ENS MYNEM ++K+VQ+MT++ I+PP +F DEI +H  +  D+FV+R ++WL+IS              S+ HSN                +K +  L       +FPLLPAS+GFC++  K+L  F
Sbjct:  885 FHAQQSFPLLTCEKDSQHDPNSNDCSPSQVCAKLCALIKSQLVKAHAEVTRRFQGDKAP--VTLSEVDNE---NVALE-EQKTNDDGNATTTGQKEEKTDGKTNEEKSEMSEP-----------------LKTNNEGFSIMSNAPNSHKYKLTMCQMTDPKFFFKTVKKELTLLRTSLPPGIWVKGFEDRTDLYSVMIRGPEKTPFEDGLFVFDFQLPSDYPRTPPLCHYLSYCSDRLNPNLYEDGKVCISLLGTWSGKGTEVWTQNSNLLQVIISIQGLILVNEPYFNEAGYEKQKGSQQGQENSRMYNEMAILKLVQAMTKMYINPPEVFVDEIKQHFAQNADRFVERLKKWLDISERHNQQVSKNICDTSAEHSNSSDPVSTAITNRNLEWEKSLVNLETIKDQPEFPLLPASRGFCLTLRKTLAAF 1291          

HSP 2 Score: 285.804 bits (730), Expect = 7.299e-79
Identity = 144/319 (45.14%), Postives = 204/319 (63.95%), Query Frame = 0
Query:  399 IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFNSTSE 717
            ++P   V+VEAL   +   V+WQDG++E+ I S++LYPIHHLDD+EFF G+FV+   + G L+ YGVVQ VD+  RIA + WFHTY    +P+P+ + +SE SVYDL+DHPDF YRPG+V I+VANF+ E++     G+V++ YP G+V VWW +   + CWPQDLYKVG+YDSDEGELWDD S           E SW   ++    GE E   +SI  +    +      EK R ++ +LEE F++    QT  +++KL EIYKDC  L+K +GTS+F    F  +++R   K      +K+A  +Q+SRLF + S+
Sbjct:  464 LKPNTKVIVEALAITSTADVVWQDGTVEQNIPSTDLYPIHHLDDQEFFPGDFVMENKEDGCLRVYGVVQSVDHAGRIAVVNWFHTYTSSENPNPTKLEQSEVSVYDLKDHPDFQYRPGTVTIRVANFEGEDAT-CTAGQVLDNYPEGRVYVWWVDGHVSMCWPQDLYKVGDYDSDEGELWDDGS----------SEGSWETESEGSSVGEEEMIDQSIKPKLAANI------EKARIAMSRLEELFTQNPALQTLDIMKKLLEIYKDCRYLDKLMGTSFFHENNFRGLLERVREKGRASVSSKVA--EQVSRLFQNPSD 763          

HSP 3 Score: 241.506 bits (615), Expect = 2.181e-64
Identity = 143/370 (38.65%), Postives = 220/370 (59.46%), Query Frame = 0
Query:   11 QLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFK---RLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIAPD---IIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREILANEMDGESKPV----DSIKLKEFDVKKEEAESKSLSVPNNSINKSNVSISVNKLR 370
            Q F+EDEVY ++KKG I FG V+ + E+++ D++ D +E    +K+G V V+W+P   E+V     + L +RSLMPGDVVRR+   GK +Q GYCR I+V T+        +VI ++NS+DL PI+ F  + AVCL  WVG I+ V+  + L F DG S+C++ D     F++++  E++++ ++   R DY+PG +L GPL   + A W +CS +LK L SK P K VKV V   +   ++VNWQC A   + +  +      IIQGEDLK+ K+L  F PCTLQIGDR +Y  KE ++ ++K +W  ++++I+   ++    P      +   K   +  E A+  +  +PN+S N  NV  SV   +
Sbjct:   31 QYFYEDEVYRINKKGFIEFGMVLENSELVSSDENSDFEEG-ARIKKGHVRVAWHPSGAEEVIQEKKVGLADRSLMPGDVVRRL-IRGKDTQRGYCRNIQV-TACVQVVGTKQVIVNVNSKDLMPIEDFTPDVAVCLDSWVGTIKTVRSKLHLVFADG-SKCIITDVEACAFEELD--ERRLSEYESSHRNDYHPGQVLYGPLEALDEAVWINCSKELKALKSK-PHKNVKVIVEAVKTDSVTVNWQCHAYSPDGACNLKEQPSYIIQGEDLKKLKLLDVFEPCTLQIGDRVYYTFKENNIQMTKDQWKAQQKQIM---LEKSPNPFKVNNKNKNNKTSKITNEGAKITNGVLPNDSNNVENVKNSVTTTK 390          
BLAST of EMLSAG00000000488 vs. Select Arthropod Genomes
Match: AAN13952.1 (uncharacterized protein Dmel_CG10254, isoform B [Drosophila melanogaster])

HSP 1 Score: 294.278 bits (752), Expect = 1.801e-81
Identity = 132/268 (49.25%), Postives = 182/268 (67.91%), Query Frame = 0
Query:  848 FKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZIS-SSYHSNKKEISPL-----------DFPLLPASKGFCVSFAKSLDEF 1103
            F++  +AP  H F  +L  P +   + + V++E  +L SSLP+G+V++ YEDR+DL S M+ GP  TPY + +FFFDFQ   DYPKSPP C YIS+C+DRLNPNLYE G+VCVSLLGTW G+  E+W+P+S +LQ+L+SIQGLILV EPY+NEAGYE Q+ T+ G ENS +YNEM +IKI QS  + L +PP IF++E++EH  E G +   R R W + S  +   N  +I  +           +FPLLP S+GFC++  + L + 
Sbjct: 1097 FQVLPNAPAAHNFISNLITPANKAQYQRAVQREYLMLKSSLPNGVVVRAYEDRMDLMSVMMVGPKRTPYQNALFFFDFQFGRDYPKSPPVCHYISYCTDRLNPNLYEGGRVCVSLLGTWMGRDNEVWSPSSTMLQVLVSIQGLILVDEPYYNEAGYEKQRGTQLGNENSRVYNEMAIIKIAQSTVKQLTNPPLIFRNELIEHFKEFGTELYARMRAWSEYSLEAQRQNITKIKDMPVEYKASCELPEFPLLPCSRGFCIAVKRVLSQL 1364          

HSP 2 Score: 218.779 bits (556), Expect = 4.340e-57
Identity = 124/319 (38.87%), Postives = 187/319 (58.62%), Query Frame = 0
Query:  370 RRRLKKRNKSVTAELYSNLPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLG-GLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANII 687
            RR++KK+ +SV   +YS  P       PK   G+ +VVE L   + V+V+WQDG++E G+ S++LYPIHHLD+ EFF G+FV +A+D+  G   YGV+Q VD+ +RIAK+ WF+ Y    +P P + G  E SVYD++DH D+ YRPG+VVI+V+NF  E+      G+VI+ YPNG+V VWWA    T C+PQDL++  E D    E  + D D  G +     EDSW          E+E  +  I S      I+ G+ ++   +++ LE+ F      + + +L  L ++YK+C  L++ + T +F    F  I+
Sbjct:  514 RRQVKKKIRSVKKTIYS--PFNKKETTPK--DGDEIVVETLVVYSSVTVVWQDGTVESGVHSTQLYPIHHLDNHEFFPGDFVTKANDISTGATDYGVIQCVDHDARIAKVMWFNIYTNIDNPIPLFKGLEELSVYDMKDHTDYQYRPGTVVIRVSNFTGEDV-NSTAGQVIDNYPNGRVRVWWAKGHITLCYPQDLFQPFEQD----EQNEQDQDVYGSDG---SEDSW----------ETESENSHISSSDNDDDILTGI-DRATEAMKVLEKMFRVLHPNRKAEVLYDLLDVYKNCRALDRLLNTKFFDEFNFKGIV 809          

HSP 3 Score: 127.102 bits (318), Expect = 1.550e-28
Identity = 97/326 (29.75%), Postives = 156/326 (47.85%), Query Frame = 0
Query:   10 AQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRM----DSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVK-----VTVLKTQVRELSVNWQCQACVGNE--SDGIAPD-------IIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQRERE 317
            +Q F ED V+ +D++G + FG V    E   +  SE+ED D   L  GEV V++YP  +E V    S+ L +R+LMPGDVVRR     + L    Q GY R++ VR    +  S   VI ++ +E L+PI  +     +C+  W+G I  +     L   +G +R  +  N    F D   +  + T+F    ++PG I+ G +            D++K L  +      +      TV   +   ++V+W C+A +GN   SD    D       +I+GE+L + K L  +    LQI DRF+ K  + D+++  + W   + E
Sbjct:   80 SQFFLEDGVFRIDRRGRVHFGLVTATTE--TYSSSEEEDVD--SLSRGEVRVAFYPGGKEMVMTEQSIGLADRTLMPGDVVRRRLPGENVLA--GQAGYVRDVNVRADVMVMGSR-MVIKNVPAERLRPISEWNQYVPLCMDTWIGTIIDINECAVLKSSNG-ARVEITSNDFHKFKDAVSECDR-TVFNPNVFFPGNIVIGRM---------PPPDRVKNLTPEITHPLTRKGRAVYTVESVETTSINVDWSCRA-IGNATGSDDTDSDPLKEPRSLIKGEELHKVKRLSLYESYELQIHDRFYLKYSKCDLLVKYADWENEQAE 386          
BLAST of EMLSAG00000000488 vs. Select Arthropod Genomes
Match: AAF56162.1 (uncharacterized protein Dmel_CG10254, isoform A [Drosophila melanogaster])

HSP 1 Score: 294.278 bits (752), Expect = 1.801e-81
Identity = 132/268 (49.25%), Postives = 182/268 (67.91%), Query Frame = 0
Query:  848 FKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZIS-SSYHSNKKEISPL-----------DFPLLPASKGFCVSFAKSLDEF 1103
            F++  +AP  H F  +L  P +   + + V++E  +L SSLP+G+V++ YEDR+DL S M+ GP  TPY + +FFFDFQ   DYPKSPP C YIS+C+DRLNPNLYE G+VCVSLLGTW G+  E+W+P+S +LQ+L+SIQGLILV EPY+NEAGYE Q+ T+ G ENS +YNEM +IKI QS  + L +PP IF++E++EH  E G +   R R W + S  +   N  +I  +           +FPLLP S+GFC++  + L + 
Sbjct: 1097 FQVLPNAPAAHNFISNLITPANKAQYQRAVQREYLMLKSSLPNGVVVRAYEDRMDLMSVMMVGPKRTPYQNALFFFDFQFGRDYPKSPPVCHYISYCTDRLNPNLYEGGRVCVSLLGTWMGRDNEVWSPSSTMLQVLVSIQGLILVDEPYYNEAGYEKQRGTQLGNENSRVYNEMAIIKIAQSTVKQLTNPPLIFRNELIEHFKEFGTELYARMRAWSEYSLEAQRQNITKIKDMPVEYKASCELPEFPLLPCSRGFCIAVKRVLSQL 1364          

HSP 2 Score: 218.779 bits (556), Expect = 4.340e-57
Identity = 124/319 (38.87%), Postives = 187/319 (58.62%), Query Frame = 0
Query:  370 RRRLKKRNKSVTAELYSNLPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLG-GLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANII 687
            RR++KK+ +SV   +YS  P       PK   G+ +VVE L   + V+V+WQDG++E G+ S++LYPIHHLD+ EFF G+FV +A+D+  G   YGV+Q VD+ +RIAK+ WF+ Y    +P P + G  E SVYD++DH D+ YRPG+VVI+V+NF  E+      G+VI+ YPNG+V VWWA    T C+PQDL++  E D    E  + D D  G +     EDSW          E+E  +  I S      I+ G+ ++   +++ LE+ F      + + +L  L ++YK+C  L++ + T +F    F  I+
Sbjct:  514 RRQVKKKIRSVKKTIYS--PFNKKETTPK--DGDEIVVETLVVYSSVTVVWQDGTVESGVHSTQLYPIHHLDNHEFFPGDFVTKANDISTGATDYGVIQCVDHDARIAKVMWFNIYTNIDNPIPLFKGLEELSVYDMKDHTDYQYRPGTVVIRVSNFTGEDV-NSTAGQVIDNYPNGRVRVWWAKGHITLCYPQDLFQPFEQD----EQNEQDQDVYGSDG---SEDSW----------ETESENSHISSSDNDDDILTGI-DRATEAMKVLEKMFRVLHPNRKAEVLYDLLDVYKNCRALDRLLNTKFFDEFNFKGIV 809          

HSP 3 Score: 127.102 bits (318), Expect = 1.550e-28
Identity = 97/326 (29.75%), Postives = 156/326 (47.85%), Query Frame = 0
Query:   10 AQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRM----DSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVK-----VTVLKTQVRELSVNWQCQACVGNE--SDGIAPD-------IIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQRERE 317
            +Q F ED V+ +D++G + FG V    E   +  SE+ED D   L  GEV V++YP  +E V    S+ L +R+LMPGDVVRR     + L    Q GY R++ VR    +  S   VI ++ +E L+PI  +     +C+  W+G I  +     L   +G +R  +  N    F D   +  + T+F    ++PG I+ G +            D++K L  +      +      TV   +   ++V+W C+A +GN   SD    D       +I+GE+L + K L  +    LQI DRF+ K  + D+++  + W   + E
Sbjct:   80 SQFFLEDGVFRIDRRGRVHFGLVTATTE--TYSSSEEEDVD--SLSRGEVRVAFYPGGKEMVMTEQSIGLADRTLMPGDVVRRRLPGENVLA--GQAGYVRDVNVRADVMVMGSR-MVIKNVPAERLRPISEWNQYVPLCMDTWIGTIIDINECAVLKSSNG-ARVEITSNDFHKFKDAVSECDR-TVFNPNVFFPGNIVIGRM---------PPPDRVKNLTPEITHPLTRKGRAVYTVESVETTSINVDWSCRA-IGNATGSDDTDSDPLKEPRSLIKGEELHKVKRLSLYESYELQIHDRFYLKYSKCDLLVKYADWENEQAE 386          
BLAST of EMLSAG00000000488 vs. nr
Match: gi|939632916|ref|XP_014286335.1| (PREDICTED: E2/E3 hybrid ubiquitin-protein ligase UBE2O [Halyomorpha halys] >gi|939632919|ref|XP_014286341.1| PREDICTED: E2/E3 hybrid ubiquitin-protein ligase UBE2O [Halyomorpha halys])

HSP 1 Score: 802.357 bits (2071), Expect = 0.000e+0
Identity = 474/1216 (38.98%), Postives = 671/1216 (55.18%), Query Frame = 0
Query:   11 QLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEG-AEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACV--GNESDGIAPDI-IQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKW-----------------LQREREILAN----------------------------EMDGESKPVDSIKLKEFDVKKEEAESKSLSVPN----------NSINKSNVSISVNKLRRRLKKRNKSVTAELYSNLPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLF--NSTSEFQNINAK----------ENSLPSNDEQDIFYE----------------------------NTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKIEAPQVEKVVVSPPEEVLSP---------VELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSN--------------KKEISPL-DFPLLPASKGFCVSFAKSLDEF 1103
            Q F+EDEVY ++KKG+I FG V+++ E+++ D++ D DE+   +K+G + V+W+P   E+V     + L +RSLMPGDVVRRM   GK +Q GYCR ++V  S  I      +I  + S+DL PI++   + AVCL  WVG I+ V   + L+  D  SRCVV D   + FD   +K    + F R +YYPG +L GPL   E  AEW   S  LK L SK   + +KVTV   ++  ++V+WQC+A    G  ++   P + IQGED+KR K+L  F PCTLQ+GDR  Y +K  D + +K +W                 L+R+R +  N                            E   E   V+   ++     KE ++ +S +  N          N  +K   S+ +  L + L K+      +  +  P   S       PG  V+VE + T +   V+WQDGS+E GI S+ELYPIHHLDD+EFF G+FV+   +   ++ YGV+Q VD+  R A +KWF TY    +P P+ + ESE SVYDL+DHPDF YRPG+VVI+VANF  E+ E    G+V++ YP G V VWW     + CWPQDLYKVGEYDSDEGELWDD +  +G         SW   ++   +   +   E+      P+++     EK R ++ +LEE F++    QT+ ++ KL E+YKDC  L+K +GT +F    F  +++R   +       ++A  DQ+ RLF  N+  E +  N            ENSL    ++D   E                            + C ++CSL   +L   +  ++ ++G     +S                       + + V P   G  K E    E +  S    + +P         V   S+P E     + GTF I ++AP  H +K+++F+P D + F K V++E KLL +SLP+GI +  +EDR+DLFS MI+GP  TPY DG+FFFDFQL  DYPK+PP C YIS+CSDRLNPNLYE+GKVC+SLLGTWSGKGTE+WT  SNLLQ+++SIQGLILV EPYFNEAGYE QK ++ G+ENS MYNEM V+K+VQSM++L+I+PPP+FK+EI +H  +  +K  +R   WL+IS +Y+SN                E  PL +FPL+PASKGFC++  K+L  F
Sbjct:    6 QYFYEDEVYRVNKKGHIEFGMVLQNSELVSSDENSDNDENG-KMKKGHIRVAWHPTGAEEVVSERKVGLADRSLMPGDVVRRMIK-GKDTQRGYCRHMEVSASIQI-VGTKHIINHVKSKDLIPIEALAPDIAVCLDSWVGGIKLVHSKLHLSLPDN-SRCVVTDPESTDFDTFYEKIDPDSEF-RGEYYPGQVLWGPLSVLEEIAEWTQPSKDLKALKSKM-KRVIKVTVEDVEIESVAVHWQCRAYSKDGAGTEKEQPKVLIQGEDIKRLKLLNVFEPCTLQVGDRNVYILKPADEVTTKEQWRKEQKRLILKENSTPLRLKRKRTVTVNSTSNTKKDDNKKKDEDKMQDNENGLDELENHKEMNNVEITNIENNQFAKESSDCESETGSNASSSATSVVSNPSSKKTPSLPIRTLTKVLHKKKLRRANQKKALRPVSLS-------PGSKVIVETVSTMSVADVVWQDGSVEVGIPSTELYPIHHLDDQEFFPGDFVIENREETCMRVYGVIQSVDHAGRTATVKWFKTYTSSENPQPALLMESEVSVYDLKDHPDFQYRPGTVVIRVANFQGED-EVCTAGQVLDNYPEGLVRVWWVEGHISMCWPQDLYKVGEYDSDEGELWDDGTSDDG---------SWQTESEDCCTCPEDNVEETSDENLKPKLVAN--IEKARIAMSRLEEIFTQNPSLQTTDVMRKLLEVYKDCRYLDKLMGTMFFHESHFQGLLERVRERGRTNLAQRVA--DQVHRLFTHNAGEEEKQGNGAVVSNSPPKDLENSLEEKSKKDTSAEFLSGSLASLEARVTKVATGGANEGLCNAHVCVKLCSLLLAQLVKAHAEVIRRFGGNSGAMS-----------------------EANNVSPSNSGNDKKEKDGSEIIKTSSDVNIPTPTNSVTEINGVIADSIPKEP----ECGTFTILDTAPNSHKYKLTMFQPTDTRVFYKRVREEIKLLRTSLPAGIWVAAFEDRMDLFSVMIRGPEKTPYEDGLFFFDFQLSADYPKAPPHCHYISYCSDRLNPNLYEDGKVCISLLGTWSGKGTEVWTSTSNLLQVIISIQGLILVNEPYFNEAGYEKQKGSQQGKENSRMYNEMAVLKLVQSMSKLIINPPPVFKEEIAQHFAQHANKLCERLESWLDISENYNSNHPLSPTTPTTYKQLHNEGVPLPEFPLIPASKGFCLTLRKTLVHF 1167          
BLAST of EMLSAG00000000488 vs. nr
Match: gi|675364278|gb|KFM57180.1| (Ubiquitin-conjugating enzyme E2 O, partial [Stegodyphus mimosarum])

HSP 1 Score: 704.131 bits (1816), Expect = 0.000e+0
Identity = 439/1232 (35.63%), Postives = 666/1232 (54.06%), Query Frame = 0
Query:   10 AQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRM---HLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQAC----VGNESDGIAPDIIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREILANEMDGESKPVDSIKLKEFDVKKEEAESKSLSVPNNSI-------------------------NKSNVSISVNKLRRRLKKRNKSVTAELYSNLPS----CYSSVIPKIRP---GEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGEL----------WDDDSD--FEGEEKDEVEEDSWVMLTKL------VGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTS--GKLNKVAGNKLAVQDQISRLFNS---TSEFQNINAKENSLPSND-----------EQDIFYE----------------------------------------NTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKI-EAPQVEKVVVSPPEEVLSPVELQSLPLEA--VLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTP-NSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYH---------------------SNKKEISPLDFPLLPASKGFCVSFAKSLDEF 1103
             + F ED VY ++KK  +  G V+ + E ++ DD E +  D      +K+G + V+W+P   E+V     +KL +RSLMPGDVVR++   GK +Q GYCR   V+    +     +V+  ++S+DL P++ F+ + AV    WVG+++ VK  + L   DG SRC++ D      DD+ D+ ++ + FK+  +YPG  L GP   F+  +W   +  + ++++  P K V+  V   +V  L+V W C+A     V N+S       + G+DLK  K++  F PCTLQIGDR +  +K+   ++S ++W  R    L   +D  + P+    LK+ + + +E ++++  V N ++                         + +  SI  N+  ++  K+      ++     S        V+ +  P   GE +V EAL T+ +  V+WQDG++E  I S+ LYP+HHLD  EFF G++VV   D    + YGVV+ VD+  R A I W  TY  G DP P  + E E SVYD++DHPDF YRPGS VI+V N + + + G   G+V++V+ +GKV V+W + +T+ C+PQ+LY+VGEYDSD+             W+ +S+    G E++  E+D       L      +   + EK++E +  EK     +    EK+R  + +LEE F++    QT  ++ +L E+YK C VL++ +GT+YF      ++++R    G++N +      + DQI RLF+S   TS+     +K+  +   D           E DIF E                                        N C+++C++ K +L   ++    ++G  +P+           + D LL+         + +LPK+       +     K + +  E + S   L +L   +    +   G F + ES P  H +K+S+F+P +P  F + V+KE  LL SSLP GI++K +EDRIDL+S MIKGP  TPY DG+FFFDFQ P DYPK PP C YIS+CSDRLNPNLYE GKVCVSLLGTW GKGTEIW P  SNLLQ+++SIQGLILV EPY+NEAGYE QK T+ G ENS MYNEMVV+K+VQ+MT+LL++PP +FK EI+EH   +  K ++R  +WL +S S++                     S+ +EI   +FPL+PASKGFC++  KSL +F
Sbjct:    5 CKFFDEDAVYKINKKKFVDVGLVLENSEFISSDDEESDGPDYKDWERMKKGHIRVAWHPNGVEEVLPEKKVKLCDRSLMPGDVVRKIVK-GKDTQLGYCRYTVVKADVQV-VGTKQVVVGVDSKDLIPMEEFVADVAVFKDSWVGMVKTVKTELTLRCCDG-SRCIMSDVDAFDMDDILDRRERASEFKKYSFYPGQQLWGPTFCFQKVKWLVLNKPMSDIINC-PNKSVRAVVEDVKVVSLAVQWVCKAYSEENVRNQSLEQPKYHVTGDDLKSIKMMNIFEPCTLQIGDRNYLIMKDSYEVISLNEWKNRMTSRL--NVDPHNSPIKRRNLKKSENENKENDAETDIVCNENMDDSSDFEDIDDDDIGKSSDTASVTSHSTASSIETNQGSQKKSKKGPHYATKMLKKKKSVRRRAMRHVMKETLPLTSGEKLVTEALITETKAHVVWQDGTVEHNILSTSLYPVHHLDSHEFFPGDYVVDGKDSRPYE-YGVVKQVDHSGRTALITWLKTYHAGGDPHPEVLSEQEVSVYDIKDHPDFKYRPGSCVIRVVNSE-DSNPGSTAGQVVDVHSSGKVEVYWVSGETSLCYPQELYRVGEYDSDDLWADDDSFSDEDSWETESEKSLVGTEENTTEKDVDGTARDLEKVSFKLEEKDDEKSTEDM-HEK-----LLANIEKLRAGMARLEEVFTQNPTLQTCNVMRRLLELYKHCKVLDQLLGTTYFHERHLQSLVERLKHRGRVNSIQ----HMADQIFRLFSSNDPTSDVNESQSKDEVISDFDKINNVSDSPKVEIDIFIESPCDAPLPTINSVNCNEDMSNRKPLLSPVSPTSGNGSEPRNLCQQLCAILKSRLLKAHEDAELRFG--MPR----------SETDALLAAE-------NELLPKDTSSKDTSQGESANKNLTNENEGLESKESLNNLEDHSSEFNSAGQGIFSMLESVPDSHKYKLSIFQPFEPCAFFRIVRKEMNLLKSSLPEGIIVKSFEDRIDLYSVMIKGPKRTPYEDGLFFFDFQFPADYPKVPPLCHYISYCSDRLNPNLYEGGKVCVSLLGTWGGKGTEIWNPETSNLLQVIVSIQGLILVSEPYYNEAGYERQKGTQQGRENSRMYNEMVVLKLVQAMTKLLVNPPEVFKSEIVEHFKTQALKLIQRLEKWLAVSESWNSEHPSSPTSPTTFKELHGNCDSSAQEIPLPEFPLIPASKGFCLTLRKSLADF 1199          
BLAST of EMLSAG00000000488 vs. nr
Match: gi|1073701723|gb|JAT93918.1| (putative ubiquitin-conjugating enzyme e2 o, partial [Amblyomma aureolatum])

HSP 1 Score: 691.034 bits (1782), Expect = 0.000e+0
Identity = 441/1250 (35.28%), Postives = 653/1250 (52.24%), Query Frame = 0
Query:   21 LDKKGNIVFGTVVRDCEMLNFDDSEDEDE--------DRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACV-----GNESDGIAPDIIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQR-------------------EREILAN-------------EMDGESKPVDSIKLKEF-DVKKEEAESKSLSVPNNSINKSNVSISVNKLRR----RLKKRNKSVTAELYSNLPSCYSSVI-----PKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYD------------------------------SDEGE-LWDDDSDFEGE--EKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCP---RVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDR--TSGKLNKVAGNKLAVQDQISRLFNST-----------SEFQNIN--------------AKENSLPSNDEQD------------------------IFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSIL--EEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKIEAPQVEKVVVSPPEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTP-NSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPL----------------------DFPLLPASKGFCVSFAKSLDEF 1103
            + +KG I FG V+ + E +    SEDEDE        DRM  K G V V+W+PK  E+V     +KL +RSLMPGDVVR++   GK +Q GYCR  +V  +  +  +N  VI ++NS DL P++ F  + A+ L  WVG+++  K  + + F DG S C++ ++     +DV D+    + FK   +Y G  L GP+R FE A W +C+ +   L+S+ P K V+VTV   +V  L V   C+A        N+SD +   +I+GE L R K++  F  C LQIGDR +Y +K  D + +  +W ++                   E E                 E + +++P       ++ DV+ EEA S S +V   S+   + + S++  RR    R  K   S+  +L        +        P ++PG+++V E L T     V+WQDG++E GI S++LYPIHHLD  EFF G+FVV  +D      Y V++ VD+  R A +  F TY  G+DP P  +G  E SVYD++DHPDF YRP S VI+VANF+++ESE    G+V++V+P GKV V W + KT+ C+PQ+LY++GEYD                              S +GE +  DDS+   E  E   ++ED     T       +   +E+      P   R  + G  E +R S+ +LEE F++    Q+ +++ +L E+Y+ C   ++ +GT+YF       +++R    G++N    +   V +QI+RLF+++            E   IN              A+    P+ +E                          +   NTC+++C++ K +L   ++ +  ++G    Q+ I   +    + D+D+      L          KE           E+ +     EV    E +            G F + +S P  H +K+S+ +PVDPK F K V+KE KLL+SSLP GI +K +EDR+DL+S +IKGP  TPY DG+F FDFQLP +YP+SPP C Y+S+CSDRLNPNLYE GKVCVSLLGTWSGKG+E+W+   S+LLQ+L+SIQGLILV EPY+NEAGYE Q+  +   ENS MYNEMVV+K+VQSM R++ + P +FKDEI +H  +  D+FV R   WL +S ++H    + +                        DFPLLPAS+GFC++  K+L  F
Sbjct:    1 ITRKGRIEFGLVLENAEYVT---SEDEDEVGANVPQWDRM--KRGHVRVAWHPKGREEVVSEKKVKLHDRSLMPGDVVRKVVK-GKDTQLGYCRNTEVLATIQVLGTN-CVINNVNSVDLVPLERFSQDVAIYLDSWVGMVQSFKTELTVRFPDG-STCILQEDDALDLEDVLDRRLPSSEFKEEGFYKGQRLCGPMRCFEAARWLNCTKETSALLSR-PHKHVQVTVDDVKVVYLFVQGVCKAYQDTSNGNNQSDELIKYVIEGEALDRVKMINIFERCALQIGDRNYYTLKASDNVTTLEEWRRQCASKYCPLVTHPVECAQGPESEAARQPAAAVASHGDTMVENNKDAQPCQDGSDSDYEDVEDEEA-SGSDTV---SVTSGSTASSMDHHRRTPAFRRGKHGPSLATKLLKKRKLRRTRKRQPLEQPAMKPGDVLVTETLATFTTAQVVWQDGTIESGIPSADLYPIHHLDGHEFFPGDFVVENTDSPPPHEYAVIKRVDHSGRTAVVTKFKTYMAGADPHPEELGMEEVSVYDIKDHPDFKYRPASCVIRVANFEDKESES-TAGQVVDVHPAGKVEVQWVSGKTSLCYPQELYRIGEYDSDDLWDDGDEEEEVDDESCSADSWETQSQRSHDGEPMSPDDSNGPSELIEPPAIDEDVAEDETAPAAVAAAAAAAEATADPSDPQSLRTKMQGQIELLRLSMARLEETFTQNPGQQSCSVIRRLLELYRHCKHFDQLLGTNYFQQRNLHVLVERIRQRGRIN----SPQHVSEQITRLFSASLDAALHDEPDIPEENAINEQLQQQQLQTTPDGAQSPPQPAKEESGQHPTAPQPRKPTSVSCRLEGSGSPLEPRNTCQQLCAILKARLLRAHEDVQVRFGMTPAQVEIAASDAASEEPDVDVEKLEESLEAAATVEAAVKES----------EESITGAVSEVAGDQESKG----------QGVFSMMDSVPDCHKYKLSILQPVDPKLFFKVVRKEIKLLTSSLPDGIHVKAFEDRMDLYSVLIKGPCRTPYEDGLFLFDFQLPAEYPQSPPQCHYVSYCSDRLNPNLYEGGKVCVSLLGTWSGKGSEMWSAETSSLLQVLISIQGLILVSEPYYNEAGYEQQRGCQQASENSRMYNEMVVLKLVQSMGRMVQNTPEVFKDEIEQHFRDNADRFVNRLESWLRLSDAWHRTSPDSAATPESLKAFAEKGDYGVAGSSAFPDFPLLPASRGFCLTLRKALTTF 1212          
BLAST of EMLSAG00000000488 vs. nr
Match: gi|939238058|ref|XP_014261696.1| (PREDICTED: E2/E3 hybrid ubiquitin-protein ligase UBE2O isoform X3 [Cimex lectularius])

HSP 1 Score: 604.364 bits (1557), Expect = 0.000e+0
Identity = 337/826 (40.80%), Postives = 470/826 (56.90%), Query Frame = 0
Query:  359 KSNVSISVNKLRRRLKKRNKSVTAELYSNLPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLF----------------------NSTSEFQN--------------------INAKENSLPSNDE----------QDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPV---LPKEEGEHKIEAPQVEKVVV-SPPEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPL----------------------DFPLLPASKGFCVSFAKSLDEFDLA 1106
            K + S+ V  L + L K+    TA+    +P+        I PG  V+VE + T +   V+WQDGS+E GI S+ELYPIHHLDD+EFF G+FV+   +   ++ YGVVQ VD+  R A +KWF TY     P P+ + E+E SVYDL+DHPDF YRPG+VVI+VANF  E+ E    G+V++ YP G+V VWW     + CWPQDLYKVGEYDSD GE WDD            +++SW   ++      ++ T    +  K    I     EK R ++ +LEE F++    QT+ ++ KL E+YKDC  L+K +GT +F    F  +++R   K       ++A  DQ+SRLF                      +S+ EF                      ++   N+ P +++          Q +   N C R+CSL   +L   +  ++ ++G     +S    +     L            + SP     P+ +    I+      +V+ +   E+ S +E+ S       N K G F I ++AP  H FK+++F+P D K+F + V+ E KLL +SLPSGI + G+EDR+DLFS MI+GP  TPY DG+FFFDFQL  DYPK+PP C YIS+CSDRLNPNLYE+GKVCVSLLGTWSGKGTE+WT  SNLLQ+++SIQGLILV EPYFNEAGYE QK ++ G+ENS MYNEMVV+K+VQ+MT+L+++PPP+F++EI  H N+  +K  +R   WL+IS  Y +N   +SP                       +FPL+PASKGFC++  K+L  F  A
Sbjct:  421 KKHPSLPVRTLTKVLHKKKLRRTAQ-KKTMPNV------NIEPGNKVIVETISTSSTADVVWQDGSVEVGIPSTELYPIHHLDDQEFFPGDFVIENREEACMRVYGVVQTVDHAGRTASVKWFKTYTSTDSPQPTLLMENEVSVYDLKDHPDFQYRPGTVVIRVANFQGED-EVCTAGQVLDNYPEGRVRVWWVEGHISMCWPQDLYKVGEYDSDGGEHWDDGPS---------DDNSWQTESEASNEENTDDTHNESLKPKLAANI-----EKARIAMSRLEEIFTQNPSLQTTEVMRKLLEVYKDCRYLDKLMGTMFFHESHFEGLLERVREKGRTNPAQRVA--DQVSRLFSHNEISGDEKIVEDVPEEKSRRDSSGEFLTGSLASLEARISASRLVSGPPVSNDTNNHPVSNQNVAANVNVQNQPVCSVNVCVRLCSLILAQLVKAHAEVIRRFGGNTGSMSEANSVSPSGPL--------TEKTETSPGANGFPELKENGDIDTKDGSNLVIPNSLAELNSTIEVNSNECIIKPNEK-GVFTILDTAPNSHKFKLTMFQPTDTKSFFRRVRGEIKLLRTSLPSGIWITGFEDRMDLFSVMIRGPERTPYEDGLFFFDFQLSADYPKTPPHCHYISYCSDRLNPNLYEDGKVCVSLLGTWSGKGTEVWTLTSNLLQIIVSIQGLILVSEPYFNEAGYEKQKGSQQGKENSRMYNEMVVLKLVQAMTKLIVNPPPVFREEINAHFNQHAEKLCQRLDSWLDISEKY-NNSHPLSPTTPTSYKDLLAEVGEVADGIALPEFPLIPASKGFCLTLRKTLVTFRQA 1212          

HSP 2 Score: 217.238 bits (552), Expect = 5.179e-54
Identity = 140/366 (38.25%), Postives = 212/366 (57.92%), Query Frame = 0
Query:   11 QLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLF-KRLDYYPGMILTGPLREF-EGAEWFSCSDKLKEL-MSKKPMKFVKVTVLKTQVRELSVNWQCQACV--GNESDGIAPDI-IQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREILANEMDGESKPVDSIKLKEFDVKKEEAESKSLSVPNNSINKSNVSISVNKLR 370
            Q F+EDEVY ++KKGNI FG V+ + E+++ DD+ D +E+   +K+G + V+W+P   E+V     + L +RSLMPGDVVRRM   GK +Q GYCR I+V  S  I      +I  INS+DL PI+    + AVCL  WVG I+ V   + L   D +S+CVV D     FD  ++K+     F  R DYYPG +L GPL    E AE    +   +++ +  K  K +KV +   ++  ++V+WQC+A    G  S+   P + IQG+D+KR K+L  F PCTLQ+GDR  Y IK  D I +K +W + ++ ++      + K  D+  +++   K   +++K L+   N+INKS    + +KL+
Sbjct:    6 QYFYEDEVYRINKKGNIEFGMVLENSELVSSDDNSD-NEECAKIKKGHIRVTWHPTGAEEVVSERKVGLADRSLMPGDVVRRMIK-GKDTQRGYCRHIEVMASIQI-VGTRHIINKINSKDLNPIEILSRDQAVCLDSWVGGIKVVHTKIHLRLPD-MSKCVVTDPDTVGFDIFDEKKDPDVDFPHRGDYYPGQVLWGPLSFLDEIAEREDPTHTREQIKLRSKNNKIIKVVIEDVEIESVAVHWQCRAYSKDGAGSEKEQPRLLIQGDDIKRLKLLSVFEPCTLQVGDRHTYTIKPTDQISTKDQWRKEQKRLILKHDSNKFK--DTPNIQKNLHKDSPSKTKKLT---NNINKSENDCNKSKLQ 362          
BLAST of EMLSAG00000000488 vs. nr
Match: gi|915662737|gb|KOC65142.1| (Ubiquitin-conjugating enzyme E2 O [Habropoda laboriosa])

HSP 1 Score: 560.836 bits (1444), Expect = 1.499e-177
Identity = 311/722 (43.07%), Postives = 419/722 (58.03%), Query Frame = 0
Query:  401 PGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEG--ELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFNSTSEFQNINAKENSLPSNDEQDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKIEAPQVEKVVVSPPEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPL-----------------DFPLLPASKGFCVSFAKSLDEF 1103
            PG  +VVE L T  + +V+WQDGS+E GI S++LYPIHHLDDKEFF G+FVV   +   +  YGVVQ VD+Q R AKIKWF TY     P P+ + E E SVYDL+DHPDF YRPG++VI++ANF+ E++ G   G+V++ YP G+V VWW +   + CWPQDLYKVGEYDSDEG  E    D+ +E E +D    D+          G  +   ESI  +    +      EK R ++ +LEE F++    QT+ ++ KL E+YKDC  ++K +  +                      G+K+ +    S + +S                           C ++C+L K +L   +  +  ++G     LS +     +K    L          I   L +E  E  IE P        PP  V S  E                F IEE+AP  H FK+++F+P DP NF + V KE KLL SSLP GI +KG+EDRIDL+S M +GP  TPY DG+F FDFQL  DYP +PP C YIS+C+DRLNPNLYE+GKVCVSLLGTWSG+GTE+WT +S LLQ+++SIQGLILV EPYFNEAG+E QK ++ G+ENS MYNEMVV+K+VQ+ T+LL  PPP+FKD I+ H      K ++R   W++IS   H+N+  +SP+                 +FPL+PAS+GFC++  K+L  F
Sbjct:  443 PGTKIVVETLSTNTKANVVWQDGSVEFGIPSTQLYPIHHLDDKEFFPGDFVVDQKEESRM--YGVVQSVDHQGRTAKIKWFKTYTSSQSPQPTLLEEREVSVYDLKDHPDFQYRPGTLVIRIANFEGEDA-GCTAGQVLDNYPEGRVKVWWVDGHVSMCWPQDLYKVGEYDSDEGGDEGVSSDASWETEMEDCFIADT---------DGTEQTELESIKPKLAAHI------EKARIAMSRLEEIFTQNPSLQTTEVMRKLLEVYKDCRYMDKLMDAN---------------------KGSKMQMHITSSNVASS-------------------------QVCVKLCTLIKAQLVLAHAEVSRRFG-----LSKMSLSDAEKGSSPLKKQKKEEEKRIE--LLQEPSECPIEIP--------PP--VYSEGE---------------GFSIEETAPDSHKFKLTMFQPTDPTNFFRTVSKELKLLKSSLPPGIWVKGFEDRIDLYSVMFRGPEKTPYEDGLFLFDFQLSADYPCAPPLCHYISYCNDRLNPNLYEDGKVCVSLLGTWSGRGTEVWTNSSTLLQVIVSIQGLILVPEPYFNEAGFEKQKGSQQGKENSRMYNEMVVLKLVQAQTKLLQHPPPVFKDIIIGHFKRHVKKLLQRLDLWMEISEQ-HNNQHPLSPVTPTTFKQISDVDEKILSEFPLIPASRGFCITLRKTLATF 1067          

HSP 2 Score: 204.912 bits (520), Expect = 3.882e-50
Identity = 117/316 (37.03%), Postives = 187/316 (59.18%), Query Frame = 0
Query:   10 AQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVV---DDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACV--GNESDGIAPD-IIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREIL 319
             Q F+ED VY +DKKGN+VFG V+ + +  +  +   + E+    K+GE+ V W+P   E++  +  + L +R+LMPGDVVRRM   GK +Q GYCR+I++ T+      + +V+ +I SEDL P++ F T+ AVC+  WVG I+     + L   DG SRCVV   D  V+   ++  D E     F   ++YPG  L GP+   + A+W +C+ +LK      P+K + V V K +   + V+W C+A    G  SD   P  +++GE+LK+ K+L  F P T+Q+GDR FY IK ++ ++++ +W +++R+I 
Sbjct:    9 CQYFYEDVVYRVDKKGNVVFGIVMENDDQ-DLSEESSDSEESPKRKKGEIRVVWHPSGVEELVSSKKVHLADRTLMPGDVVRRMIK-GKDTQRGYCRDIEL-TACVQVIGSKQVLTNIRSEDLVPLEEFTTDIAVCMDSWVGGIKMAHFKLWLTTPDG-SRCVVNEMDSRVLGQLEERRDNEHD---FPHSEFYPGQSLWGPVHCLDHAQWITCTRELKAKRKIIPLKLINVIVEKVETDWVGVHWLCRAYSKDGAWSDQAQPKFVVEGENLKKLKLLNVFEPSTVQVGDRNFYVIKGDENVITREQWRKQQRDIF 317          
BLAST of EMLSAG00000000488 vs. nr
Match: gi|357617902|gb|EHJ71057.1| (hypothetical protein KGM_13479 [Danaus plexippus])

HSP 1 Score: 533.102 bits (1372), Expect = 4.620e-169
Identity = 305/792 (38.51%), Postives = 448/792 (56.57%), Query Frame = 0
Query:  405 VVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVR-ASDLGGLQS-----YGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKL--------------------VGSGESEKTSESIVSEKC---PRVI---VFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTS--GKLNKVAGNKLAVQDQISRLFNST--------------------------------SEFQ------NINAKENSLPSND-------------EQDIFY---ENTCRRICSLFKLKLAYVY-DVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDD--ISPVLPKEEGEHKIEAPQV-EKVVVSPPEEVLSP-VELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPLDFPLLPASKGFCVSFAKSLDEF 1103
            VVVE L T +  +V+WQDG++E GI S++LYPIHHLD +E F G+FV+  A+ +   Q      YGVVQ VD+  R A + W+ TY    +P P  + ESE SVYDL+DHPDF YRPG+VVI+VANF  E++     G+VI+ +P G+V VWW +  T+ CWPQDLYKVGEYDS++GELW  +          V EDSW   +                        S  +  T+ +  SE+    P+++   V    E+ R +++KLEE F++    Q+  ++ KL  +YKDC  L++ +GT++F  + F  +++R    G     AG +  V +Q++RLF++                                 +EF       N+N ++++  S+              E ++ Y    N C ++C+L   +L   + +V   +   +   L+ L + Q      +    + + Y+   +S V   E+ E K    +V E V  +P  E   P   L     E         F + ESAP  H F++S+ +P +P++F   VK+E KLL S LP G+ ++GYEDRIDL S MI GP+ TPY  G+F FD QL  +YP++PP C Y S+CSDRLNPNLYE+GKVCVSLLGTWSG+G E+W  +S+LLQ+++S+QGLIL  EPYFNEAGYE QK T+ GEENS MYNEMV++K+VQSMT+++++PP  F++EIL+H+        +R    + +S   +    ++ P D+PL+PAS+GFC++   SL+ F
Sbjct:  186 VVVETLHTTSRANVVWQDGTIEMGIPSTQLYPIHHLDGQECFPGDFVINGAASVEETQQLKHREYGVVQRVDHHGRTAIVHWYRTYTSVDEPVPQMLFESEMSVYDLKDHPDFQYRPGTVVIRVANFTGEDA-NCTAGQVIDNFPTGRVKVWWVDGHTSMCWPQDLYKVGEYDSEDGELWGSEGT--------VSEDSWETQSSAHELDPRTPDAPVAAPPATAPTWSAPASGTAVATPSEEAGPLPKLLEPRVAAHIERGRVAMRKLEEIFAKQPTLQSQEIMRKLLNLYKDCRFLDRLMGTTFFHEDHFLGLLERVRERGASTPRAGER-RVHEQLARLFSTEPAADLEHDEDMKMEDRTLRPIVAANVTVEPMETEFSSPKKPLNLNIEQSASCSDTNVAEGPIENSAETEGEVVYSGSRNVCYKLCALIHRQLVKAHAEVNRRRPQELAEFLNSLMKKQKGGGEKLPYHFS-VQYEALVVSMVDQAEKAEEKASTHEVKEPVADTPKMETAGPEAPLACAEGEGEGGVGGEGFSVLESAPAAHRFRLSMLQPSEPRSFYSAVKREIKLLKSDLPPGVWVRGYEDRIDLISVMIAGPARTPYEGGLFVFDVQLGGEYPRAPPLCHYHSYCSDRLNPNLYEDGKVCVSLLGTWSGRGVEVWGKDSSLLQVIVSLQGLILNAEPYFNEAGYEKQKGTQQGEENSRMYNEMVLLKLVQSMTKMVMNPPEPFREEILQHLRASAASLCRRLEGLVSLS---NGQPCDLPPPDYPLIPASRGFCLTLRSSLESF 963          
BLAST of EMLSAG00000000488 vs. nr
Match: gi|913300714|ref|XP_013199731.1| (PREDICTED: E2/E3 hybrid ubiquitin-protein ligase UBE2O isoform X2 [Amyelois transitella])

HSP 1 Score: 528.865 bits (1361), Expect = 5.323e-164
Identity = 309/802 (38.53%), Postives = 453/802 (56.48%), Query Frame = 0
Query:  402 GEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVR-ASDLGGLQS-----YGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDS-------DFEGEEKDEVEEDSW---------VMLTKLVGSGESEKTSESIVSEK-----------CPRVI---VFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTS--GKLNKVAGNKLAVQDQISRLF---------------------------------NSTSEFQ---NINAKENSL--------------PSNDEQ---DIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKIEAPQVEKVVVSPPEEV--LSPVELQSLPLEAV----LNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPLDFPLLPASKGFCVSFAKSLDEFDLA 1106
            G+ VVVE L T +  +V+WQDG++E GI S++LYPIHHLD +E F G+FVV  A+++   Q      YGVVQ VD+  R A + W+ TY    +P P  + ESE SVYDL+DHPDF YRPG+VVI+VANF  E++     G+VI+ YP G+V VWW +  T+ CWPQDLYKVGEYDS+EGELW  D+       + +    DE E  +          V++  +      E  S +   +K            PR++   V    E+ R ++++LEE F++    Q+  ++ KL  +YK+C  L++ +GTS+F  + F  +++R    G     AG +  V +Q++RLF                                 N+T + Q   N++A + S               PS+ EQ   +    N C ++CSL   +L   +  +  +YG +V  +S+ EE + DK  D +                +E       A + E   V  P E   L+  E    P   V           F + +SAP +H+F++S+ +P DP++F   VK+E KLL S LP G+ ++G+ DRIDL S MI GP++TPY  G+F FD QL  +YP++PP C Y S+C+DRLNPNLYE+GKVCVSLLGTWSG+G E+W  +S+LLQ+++S+QGLIL  EPYFNEAGYE QK T+ G+ENS MYNEMV++K+VQSMT+++++PP  F++E+  H+ ++     +R    + +S    +   ++ P +FPL+PAS+GFC++   SLD F  A
Sbjct:  503 GDRVVVETLHTTSRANVVWQDGTIEMGIPSTQLYPIHHLDGQECFPGDFVVSGAANVEENQQLKHRVYGVVQRVDHHGRTAIVHWYRTYTSADEPVPQMLHESEMSVYDLKDHPDFQYRPGTVVIRVANFTGEDTHC-TAGQVIDNYPTGRVKVWWVDGHTSMCWPQDLYKVGEYDSEEGELWASDASGSEDSWETQSSAHDEREPQALLPAPETPQQVIVNPMSQEAALEAPSVTSTRDKPAPAAQRTEAAVPRLLEPRVAAHLERGRLAMRQLEEMFAKHPTLQSQEIMRKLLNLYKECRFLDRLMGTSFFHEDHFLGLLERVRERGATTPRAGER-RVHEQLARLFSPNEGEVPANMLAEHERSRENEASEPVEPMEVENTTPKKQLHLNLDAAQPSGSDLPPSQDKATEENPSSSEQTEGEGSGRNVCYKLCSLIHSQLIKAHAEVSRQYGALV--VSMAEEEKGDKPNDEV----------------QESSSAASNAAKSEAGDVPVPTETAPLATAETADEPEPEVPGGTEQEGGEGFYMLDSAPADHSFRLSMLQPSDPRSFYSAVKREIKLLKSDLPPGVWVRGFMDRIDLLSVMIAGPANTPYEGGLFVFDVQLGGEYPRAPPLCHYHSYCTDRLNPNLYEDGKVCVSLLGTWSGRGVEVWGKDSSLLQVIVSLQGLILNAEPYFNEAGYEKQKGTQQGKENSRMYNEMVLLKLVQSMTKMVMNPPEPFREEVAAHMRQQAGALCRRLEALVALSRG--AAPPDVPPPEFPLVPASRGFCLTLRSSLDSFRAA 1282          

HSP 2 Score: 202.601 bits (514), Expect = 2.854e-49
Identity = 116/324 (35.80%), Postives = 182/324 (56.17%), Query Frame = 0
Query:   11 QLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIAPD----IIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREILANEMDGESKPV 330
            Q F+EDEVY ++ +G +VFG V+ + E ++ D   D +     +++GE+ V W+P   E+V    S+ L +RSLMPGDVVRR+ + GK +Q GYCR+I V T+A        VI  + SE L+P++ F  + AVCL  WVG  + V   + L   DG SR    D      +D + + ++ T +   ++YPG ++ GPL   + A W   + ++K     K M   K TV       +SV+WQC+A   + +D   P     +++GEDLK  K+L  F PCTLQ+GDR F  I  +D  +SK +W +++ +I  + +  +++PV
Sbjct:   13 QYFYEDEVYKINNRGQVVFGLVLENYEGISSDQESDIE---AAVQKGEIRVVWHPSGTERVISEKSVGLADRSLMPGDVVRRLIA-GKDTQRGYCRDI-VMTAALQIVGTKHVIPSVASERLQPLEEFTPDLAVCLDSWVGTSKAVHSKLRLVSTDG-SRLEYPDLDNCPLEDYSMRRRRSTPYSSSEFYPGQVVYGPLGSLDNANWLHITKEMKAARKHK-MHDHKFTVEAVVTESVSVHWQCRALCTHPADTNGPQQPKFLVEGEDLKNLKLLNVFEPCTLQVGDRNFLTIGPDDTFVSKKQWRKQQSKIYRS-LRKQTRPV 328          
BLAST of EMLSAG00000000488 vs. nr
Match: gi|1080049426|ref|XP_018566113.1| (PREDICTED: (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O [Anoplophora glabripennis])

HSP 1 Score: 492.271 bits (1266), Expect = 2.024e-152
Identity = 368/1157 (31.81%), Postives = 576/1157 (49.78%), Query Frame = 0
Query:    7 EEIAQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRC-VVDDNVISTFDDVND----KEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIAPDI-IQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWL-----------QREREILA-NEMDGESKP-VDSIKLKEFDVKKEEAESKSLSVPNNSINKSNVSISVNKLRRRLKKRNKSVTAELYSNLPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLG-GLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLY---KVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFAL-------------EKFANIIDRTSGKL-------NKVAGNKLAVQDQISRLFNSTSEF-----QNINAKENSLPSN----DEQDIFY---ENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKIEAPQVEKVVVSPPEEVLSPVELQSLPLEAVLNFKNG-----TFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPLDFPLLPASKGFCVSFAKSLDEF 1103
            +++ + +  D VY  DK   I+FG V+      +++ S D DE    L++G++ V W     E+V   S ++L NRS++PGD+VRR++  GK +Q GYC+E K   +  I    D+VI  + S+ L  ++ +  + AVCLG   G I  ++  +++      S+C  V D + S   D++D    K  +I+      +YPG  L      FE   W   S  +K  +  +     + TV   +   + + W       N + G +    I  +D+K+ K+L +     L++ +R   K+   DV++ K  W+           Q+ R I++ N       P + S  L    ++ EE E  +     +  N S+ S + +  +   K+       EL                PG  + +E +  +++++V+WQDGS E+ I S++LY    LDD EFF GE+V   +  G   + YGVVQ+V+Y  R A +KWF TY   ++  P+ + ++E SVYDL+ H  F +RPGS+V       ++ ++    G+VI+    G V V W +     CWPQD+    +  EYD  + E  D+D+     E + +E            S   + + E+++     R+      + +R  +  L+E F + +  +  + L+ L  +Y++   L+K +GTS+F+L             EK   +     G+L         ++  KLA ++ I+++    +       +   +KE ++P+     D  ++ Y   +N C  + S+ K+++   Y  I+ + G     LS+L   +  +++    S T L      P  P                  + P+E  S +     PL    N K G      + I E AP  H F    FEP D + FLK V+KE KLL  SLP G+ ++ Y +RIDL S MI+GPS TPY DG+F FD QL PDYP+SPP   YIS+ ++RLNPNLY EGKVCVSLLGTW G+GTE+W P S LLQL++SIQGLILV EPY+NEAGYE Q  T+ G ENS  YNE+V++K+VQSMT +L+SPP +FK EI  H  + G     R  +       +      + P DFPLLP SKG  +S + +L+ F
Sbjct:    6 DKVKEFYSNDIVYRFDKHKRIIFGVVID-----SYEASSDVDEYHA-LQKGQIRVLWSNNSREQVWRQSKVRLMNRSVIPGDIVRRLEK-GKETQRGYCKETKQLATVQI-VGTDKVIEHVYSDRLHSVRPYDVDDAVCLGSKFGRIEAIEQMISMQ-----SKCGSVVDVLTSINHDLDDYWLSKRNRISFDA---FYPGQELICIPSNFEQPHWVKKSKAMKRNIQARQ----RFTVQNVEDILIEIAWY------NNTTGYSHITEISQDDIKKLKVLEHPSDTCLELAERRLLKLSACDVLIKKKDWIRKLSVSYRPDTQKVRHIVSCNTKTAPRVPKIRSSSLLYACIESEEDEWWTEEGEESDDNGSSSSQNTSISKSSKKRHYPPKPRELV---------------PGHTLPIEVICVESKITVVWQDGSEEKDIPSTQLYYSISLDDHEFFPGEWVTSDTIKGECTEKYGVVQNVNYLERTACVKWF-TYS-ENEKLPTELTKNEMSVYDLKKHSKFVFRPGSIV------KSKPAQDDKMGKVIDSCIEGYVKVQWLDGNEDNCWPQDIELIPETAEYDFSDEESSDEDAACVSWETESIE------------SYAGDLSDETVLQNMAARL------DFVRNRIIYLKEAFKQHTITENFSFLKDLLLVYENSSYLDKLLGTSFFSLKSKHFQTLLMNAKEKAKTLGVELRGRLFSGDNLCPSISKIKLAEKENINKMIKLENRINAQIEKKEGSKEATVPTTPLTPDSTEVTYMSQDNLCVELLSMLKMRMDLAYAEIISRIGG-TQALSVL--TKASENVATPASSTPL------PSCP------------------TTPDESFSALS----PLRPKFNDKMGEIENEPYTIIEEAPVMHHFYSGKFEPTDLQRFLKAVQKEYKLLKESLPPGVWVRSYGNRIDLLSVMIQGPSKTPYEDGLFLFDIQLSPDYPRSPPLVHYISYSTERLNPNLYVEGKVCVSLLGTWMGRGTEVWGPKSTLLQLIVSIQGLILVSEPYYNEAGYEKQTDTQQGYENSRTYNELVILKLVQSMTEMLLSPPEVFKGEIFSHFAKNGRSLCDRLNK-------FCGETDAVVP-DFPLLPVSKGLKLSLSAALNSF 1056          
BLAST of EMLSAG00000000488 vs. nr
Match: gi|91084477|ref|XP_971283.1| (PREDICTED: uncharacterized protein LOC659924 [Tribolium castaneum] >gi|270008871|gb|EFA05319.1| hypothetical protein TcasGA2_TC015477 [Tribolium castaneum])

HSP 1 Score: 483.797 bits (1244), Expect = 1.332e-149
Identity = 365/1141 (31.99%), Postives = 569/1141 (49.87%), Query Frame = 0
Query:    9 IAQLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDG----ISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACVGNESDGIAPDIIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQRER-EILANEM-DGESKPVDSIKLKEFDVKKEEAESKSLSVPNNSINKSNVSI-SVNKLRRRLKKRNKSVTAELYSNLPSCYSSVIPKIRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDD-DSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEK--FANIIDRTSGK-----------------LNKVAGNKLAVQDQISRL--FNSTSEFQNINAKE---NSLP----SNDEQDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVLPKEEGEHKIEAPQVEKVVVSP--PEEVLSPVELQSLPLEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPLDFPLLPASKGFCVSFAKSLDEFDLAHSKYL 1111
            + + +  D VY  D++  IVFG V    E  + D++         L++G++LV+W     +++   S + L  R + PGD+VRR++  GK +Q GYC++ K   +  I   +D+VI  +    L  ++ F    AVCLG   G I+ +   + +    G    + R +  +     F  +N    +         YPG  +          +W     K K +M K  +   K T+   ++  + V W         ++      ++ +++KR K+L Y     L +GDR+F K+K  D++L K  W++++  + L +++   ++  + +   + FD   +    +       S NK  +SI  ++K+++R K          Y NL         K   G IV V+     A V+V+WQDG+ E+ I ++ELY     DD EFF GE+VV  S+      YGVVQ V++Q R A+IKWF +  + ++  P  +  +E SVYDL+ H  F +RPGSVV      +++       G+VI+  P G V V W   K   CWPQ++  + E  S++ E  D  DSD      D++ ED++   T+ V S + + T E  +     RV      + IR  +  L E F   +  Q    ++ L  IY +   L+K +GTS+F+ E   F  ++  T  K                 L+     K A +D I ++    S    Q    KE    S P    S++  +   EN C  +  + K+++  +Y  I+ + G                      ++T LT    +P+ P            ++  +++P  P +V  P+EL  +P       KN  F I E AP  H +  S F P D + FL  ++KE KLL  SLP+G+ ++ Y++R+DL S MI+GP+ TPY DG+F FD QL PDYPK+PP   YIS+ ++ LNPNLY EGKVCVSLLGTW G+G+E+W PNS LLQL++SIQGLILV +PYFNEAGYE Q  T+ G ENS  YNE V++K+VQSMT LL + P +F++E+L H   +G+   +R  ++         +++ + P +FPLLP SKGF +S   +L+ F  A  K L
Sbjct:    6 VKEFYAHDLVYKFDERKRIVFGVVSESYESSDSDET-------CALQKGQILVTWSNPTRKRICRQSKVYLLCRCIAPGDIVRRLED-GKETQRGYCKKTKQFATVQI-VESDKVIEHVPDSRLCFVKPFQVGDAVCLGDKFGRIQSIDEMIRMQSKCGSIVEVRRSINYNIRDYWFHSINGAYHEAE-------YPGQTVFCTPMHLRDPKWIK---KSKSMMRKMEIG-QKFTIQSIEICLIEVWWYSPNGHTEYAE------LKDDEIKRLKVLPY--SENLVMGDRYFLKLKPTDILLKKRDWIKKKSLQSLPHKVPKSDNSTLTNTCAEPFDADDDWCTEED----EESDNKGEISIYGLHKIKQRKKT---------YPNLN--------KFSSGSIVAVQVFCADARVTVVWQDGTEEKDIPTTELYYSVSQDDHEFFPGEWVVSNSNENS-DKYGVVQRVNFQERTAEIKWFSS--VDANQEPQELATNESSVYDLKKHVKFVFRPGSVVKAKPTVEDKM------GKVIDSCPQGYVIVQWLGGKRENCWPQNIELILE--SEDFEFPDAFDSD------DDLSEDAYSWETESVESFDGDVTDEINLQNMAARV------DFIRNRISYLREAFKHYNITQNFHFVKDLLCIYDNSGYLDKYLGTSFFSTESKHFQALLLETKKKAKSLGIELRGRMFSRENLSCPTKMKNAEKDNIQKMIKLESKVNVQIEMCKECSGESCPTTPDSSEPAEKPQENLCVELLVMLKMRMDLIYAEIISRIGGR-------------------QALTVLTNSSENPLKPSPST-----TTALQNALLTPETPPKVAKPIEL--IP-------KNEAFSILEEAPDTHRYFSSKFVPKDLQKFLVALQKEYKLLKDSLPAGVWVRTYDNRMDLLSVMIRGPAKTPYEDGLFLFDIQLSPDYPKNPPGVHYISYITEPLNPNLYVEGKVCVSLLGTWMGRGSEMWGPNSTLLQLIVSIQGLILVAQPYFNEAGYERQTHTQQGCENSRTYNEFVILKLVQSMTELLNAAPKVFQNEVLAHFQAKGEAMCERLMKY--------CDEEPLVP-EFPLLPVSKGFKLSLGSALNGFRQALKKAL 1032          
BLAST of EMLSAG00000000488 vs. nr
Match: gi|817068940|ref|XP_012256424.1| (PREDICTED: E2/E3 hybrid ubiquitin-protein ligase UBE2O [Athalia rosae])

HSP 1 Score: 477.633 bits (1228), Expect = 1.645e-144
Identity = 284/766 (37.08%), Postives = 428/766 (55.87%), Query Frame = 0
Query:   11 QLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRMHLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRL-DYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQACV--GNESDGIAPD-IIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREILANEMDGESKPVDSIKLKEFD-----VKKEEAESKSLSVPNNSINKSNVSISVNKLRRRLKKRNK--------------SVTAELYSNLPSCYSSVIPKIR---------------------------PGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLGGLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDSDEGELWDDDSDFEGEEKDEVEEDSWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFNSTSEFQNINAKEN 726
            Q F+ED VY +DK+GN+ FG V+ + +  + D+S D +E     K+GE+   W+P   E++  +  + L +RSLMPGDVVRRM   GK +Q GYCR+I++ T+        +V+ ++ SEDL P++ F  + AVC+  WVG I+ V   + LA  DG SRCV+++      D   +K +    F  L ++YPG  L GPL   E A+W  C+ +LK     KP K  KV V K +   + V+WQC+A    G   D   P+ ++QG+DLK+ ++L  F P TLQ+GDR FY +KE + ++S+ +W +++REI      G  K    I  K  D     + ++ A+    ++ NN IN ++ S++ N L    +  N               S TA + S+  SC +S   K +                           PG  +VVE L T  + +V+WQDGS+E GI S++LYPIHHLDD+EFF G+FVV   +   +  YGVVQ VD+Q R AK+KWF TY     P P+ + E E SVYDL+DHPDF YRPG++VI++ANF+ +++ G   G+V++ YP G+V VWW +   + CWPQDLYKVGEYD DEGELWDD S     E +   ED ++  T+  G+ ++E     + +            EK R ++ +LEE F++    QT+ ++ +L E+YKDC  ++K + TS+F    F  +++R   +       ++A  DQ++RLF   S    I+ +++
Sbjct:   10 QYFYEDVVYRVDKRGNVEFGIVMANDDPDSSDESSDNEESPKR-KKGEIRAVWHPSGVEELVNSKKVNLADRSLMPGDVVRRMIK-GKDTQRGYCRDIEL-TACVQVMGTKQVLTNVKSEDLVPLEEFTADIAVCMDSWVGGIKMVHSKLWLAMPDG-SRCVINEIDACALDQFEEKRENDNDFPHLLEFYPGQSLWGPLHCLEDAQWILCTKELKAKRKSKPQKITKVVVEKVETDSVGVHWQCRAYSKDGAGMDQAQPNFVVQGDDLKKLRLLNVFEPSTLQVGDRNFYVVKENENVISREQWRKQQREIFNIPKHGPRKSRVQIITKPADWKSKNIDRQTADRMEANLANNPINNTHNSVTNNSLIPPPQNTNAESSDDWDTEDTGSLSDTASVSSDC-SCVTSTGKKKKGPALMTKVLKKKKLCKAKKKVAPVPLVPGTKIVVETLSTSTKANVVWQDGSVEIGIPSTQLYPIHHLDDQEFFPGDFVVDQKEESRM--YGVVQSVDHQGRTAKVKWFRTYTSSQSPQPTMLQEREVSVYDLKDHPDFQYRPGTLVIRIANFEGQDA-GCTAGQVLDNYPEGRVKVWWVDGHVSMCWPQDLYKVGEYDGDEGELWDDVSSEASWETE--SEDCFIADTE--GAEQTENIKPKLAAH----------IEKARIAMSRLEEIFTQNPSLQTTEVMRRLLEVYKDCRYMDKLMATSFFHESHFQGLLERVRERGRANVAQRMA--DQVTRLFTHQSGGTEIDPEQD 751          

HSP 2 Score: 343.584 bits (880), Expect = 8.040e-96
Identity = 158/298 (53.02%), Postives = 211/298 (70.81%), Query Frame = 0
Query:  829 PVELQSLPLEAVLN------FKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNKKEISPL-----------------DFPLLPASKGFCVSFAKSLDEF 1103
            P+EL S+P E  ++       +   F IE++AP  H FK+++F+P D  NF + V KE +LL +SLP GI +KG+EDR+DL+S MI+GP  TPY DG+F FDFQL  DYP +PP C YIS+CSDRLNPNLYE+GKVCVSLLGTW+G+GTEIWT +S LLQ+++SIQGLILV EPYFNEAGYE QK ++ G ENS MYNEMVV+K+VQ+ T+LL  PP +FK+ I++H      K ++R   W++IS   H+N+  +SP+                 +FPL+PAS+GFC++  K+L  F
Sbjct:  934 PIELLSVPSEPSVDIPPPVYMEGEGFSIEDTAPDSHKFKLTMFQPTDASNFFRTVSKELRLLRNSLPPGIWVKGFEDRMDLYSVMIRGPEKTPYEDGLFLFDFQLSADYPAAPPLCHYISYCSDRLNPNLYEDGKVCVSLLGTWAGRGTEIWTSSSTLLQVIVSIQGLILVSEPYFNEAGYEKQKGSQQGRENSRMYNEMVVLKLVQAQTKLLQHPPLVFKETIIQHFKRHAQKLLQRLDLWMEISEQ-HNNQHPMSPITPTTFKEIADYDDKILPEFPLIPASRGFCITLRKTLATF 1230          
BLAST of EMLSAG00000000488 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold703_size109190-snap-gene-0.18 (protein:Tk04144 transcript:maker-scaffold703_size109190-snap-gene-0.18-mRNA-1 annotation:"ubiquitin-conjugating enzyme e2-")

HSP 1 Score: 448.743 bits (1153), Expect = 3.086e-138
Identity = 272/732 (37.16%), Postives = 424/732 (57.92%), Query Frame = 0
Query:   11 QLFHEDEVYHLDKKGNIVFGTVVRDCEMLNFDDSEDEDEDRM----HLKEGEVLVSWYPKYEEKVHLNSSLKLGNRSLMPGDVVRRMDSLGKRSQTGYCREIKVRTSAAIKCSNDRVIYDINSEDLKPIQSFLTNSAVCLGPWVGVIRKVKLNVALAFHDGISRCVVDDNVISTFDDVNDKEQKITLFKRLDYYPGMILTGPLREFEGAEWFSCSDKLKELMSKKPMKFVKVTVLKTQVRELSVNWQCQAC-----VGNESDGIAPDIIQGEDLKRTKILGYFFPCTLQIGDRFFYKIKEEDVILSKSKWLQREREILANEMDGESKPVDSIKLKEFD-VKKEEAESKSLSVPNNSINKSNVSISVNKLRRRLKKRNKSVTAELYSNLPSCYSSVIPK-----------IRPGEIVVVEALRTKAEVSVLWQDGSLEEGIDSSELYPIHHLDDKEFFTGEFVVRASDLG-GLQSYGVVQHVDYQSRIAKIKWFHTYQIGSDPSPSYVGESEESVYDLRDHPDFNYRPGSVVIKVANFDNEESEGGLGGEVIEVYPNGKVTVWWANKKTTECWPQDLYKVGEYDS-DEGELWDDDSDFEGEEKDEVEED----SWVMLTKLVGSGESEKTSESIVSEKCPRVIVFGLFEKIRTSLQKLEENFSEASKPQTSAMLEKLWEIYKDCCVLEKSIGTSYFALEKFANIIDRTSGKLNKVAGNKLAVQDQISRLFNST 715
              F+EDEVY L + G + FG VV + E  + D+ ED++E        LK G V V+W+PK  E+V   + L L +RSLMPGDVVRR    GK +Q GYCR +KV  +  I     +V+  I+S DL+ +++F  +  +C   W+GVI++VK  + L   DG S C +D+++  + +++ +   +   F   +Y+PG++L GP   +E  E+ S S   +     K  KF++ TVL+T+V  + V+WQC+A      V   S  I    +  ++LK+ K L  F P TLQIGDR FY + E D +++KS+W + E+  L    +G  +PV  ++ K+ D V + E ES + S   +S   S+  +S     ++ K +  +V+++      +      PK           I+ GE  VVE +RT++   V+WQDG++EE + S EL+PIHHLD++EFF G++V R  D       YGV+Q VD+  R  ++KWF TY  G++P P  +  +EE VYDL+DHPDF YRP S+VI+VANF++E+S    GG+V++   +GKV VWWA++  +ECWPQDLYK+GEYDS DEG LW  D+D    E DE  +D    SW  L++    G+  K +  + + + P++      EK R ++ +LEE F E    QT+ ++++L ++YKDC  +++ +GTS+F    F  ++++   +  KV   +  V  Q+ RLF+ +
Sbjct:   20 HFFYEDEVYRLTRNGQLQFGMVVENSEYYSSDEDEDDEESDEGEEEKLKPGMVRVAWHPKGNEQVIPEAKLGLADRSLMPGDVVRRF-VRGKDTQGGYCRNVKVFATVQI-VGTTQVVTRISSGDLENLEAFNADVGICYESWIGVIKEVKNKITLKLSDG-SICALDEDLADSLENLCEDRDEDCQFNGFEYFPGLVLKGPKSIYEKGEFLSKSPDYE-----KKSKFMRSTVLETRVESVGVHWQCKAYNEDNDVSGSSSKIPDYSLTKDELKKVKKLYVFGPSTLQIGDRNFYTLTESDTVMTKSEWNRLEQ--LHKRAEGTKEPVKKVEPKKVDNVGEPERESANDS---SSSEGSDAELSSEGEAKKGKPKRMAVSSKAMHKKKTKMRRAKPKQEKRRFPRLKTIKVGEKYVVETIRTQSFADVIWQDGTVEENVSSKELFPIHHLDEQEFFCGDYVCRNDDAHPNPHEYGVIQRVDHAGRTCQVKWFKTYTAGNEPRPLELDTNEEPVYDLKDHPDFRYRPSSIVIRVANFEDEKS-SCFGGQVLDNLTSGKVMVWWADEAVSECWPQDLYKIGEYDSDDEGGLWSGDADDWLNEDDEDGDDRSLSSWETLSETSEPGDVAKENVDVTNLR-PKLAAN--IEKARIAMTRLEEIFHENPNLQTNFVMKQLLDVYKDCRYVDRLMGTSFFHEHNFEGLLEKIRDR-GKVGTYQQQVNAQLRRLFDRS 733          

HSP 2 Score: 385.571 bits (989), Expect = 4.496e-115
Identity = 214/455 (47.03%), Postives = 276/455 (60.66%), Query Frame = 0
Query:  701 KLAVQDQISRLFNSTSEFQNINAKENSLPSNDEQDIFYENTCRRICSLFKLKLAYVYDVIVEKYGTMVPQLSILEEIQHDKDLDILLSITGLTYDDISPVL---------PKEEGEHKIEAPQVEKVVVSP-------PEEVLS----------PVELQSLP-------------LEAVLNFKNGTFKIEESAPQEHTFKVSLFEPVDPKNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQSMTRLLISPPPIFKDEILEHINERGDKFVKRWREWLZISSSYHSNK----KEISPLDFPLLPASKGFCVSFAKSLDEFD-----LAH 1107
            KLA  +Q+++  +   +FQ  +      P +D+Q +     C R+CSL K +L    + +VE+YG      +     Q D     L    G   DD+  ++         P       I+  + E  V SP       P+ V S          P + +S P             + +        F IEESAP  H FK+SLF+P+DPK+F+KFV+KE  LL ++LP GIV+K +EDR+DL+S MIKGP +TPY DG+FFFDFQL  +YPK+PP C YIS+C+DRLNPNLYE+GKVCVSLLGTWSGKGTE WTPNSN+LQLL+SIQGLILV EPYFNEAGYE QK T+ G ENS MYNEMVV+K+VQS TRL+I+PPP FK  I EH  +RG  FV R  +WL +S    SN+    +EI   +FPL+PASKGFC+S  K++  F      LAH
Sbjct:  825 KLAEANQLAQ--DMLVDFQGAHQ-----PWDDDQQV-----CARLCSLMKAQLVKALEEVVERYGGQGAMFT-----QED-----LKESPGEAEDDLEGIMGQILSSDRDPSASNSTDIDGAEWEPSVTSPMGKGAGIPKGVSSQDIAQALYGTPTKTESEPNPSGQIAEEMDDEMASESAASTEVFSIEESAPNCHKFKLSLFQPIDPKSFIKFVRKEVNLLKTNLPEGIVVKAFEDRMDLYSVMIKGPRNTPYEDGLFFFDFQLTAEYPKTPPACSYISYCTDRLNPNLYEDGKVCVSLLGTWSGKGTETWTPNSNMLQLLISIQGLILVSEPYFNEAGYERQKGTQQGRENSRMYNEMVVLKLVQSTTRLIINPPPTFKTNITEHFEKRGQMFVDRLEQWLNLSMKIQSNEVLDGEEIP--EFPLIPASKGFCISVRKAVQRFKEVLSTLAH 1255          
BLAST of EMLSAG00000000488 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold865_size87005-snap-gene-0.24 (protein:Tk05467 transcript:maker-scaffold865_size87005-snap-gene-0.24-mRNA-1 annotation:"ubiquitin-conjugating enzyme e2 a")

HSP 1 Score: 72.7886 bits (177), Expect = 4.498e-15
Identity = 45/152 (29.61%), Postives = 74/152 (48.68%), Query Frame = 0
Query:  879 KEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEENSWMYNEMVVIKIVQS 1030
            ++ K L    P+G+     ++ I L++A+I GP DTP+ DG F    Q   +YP  PPT   + F S   +PN+Y +G +C+ +L          W+P  ++  +L SIQ L+    P        +Q +    +EN   Y + V + + QS
Sbjct:   11 RDFKRLQEDPPAGVSGAPGDNNIMLWNAVIFGPHDTPFEDGTFKLTIQFTEEYPNKPPT---VRFISKMFHPNVYADGGICLDIL-------QNRWSPTYDVSAILTSIQSLLDEPNPNSPANSVAAQLY----QENRREYEKRVALIVEQS 148          
BLAST of EMLSAG00000000488 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold624_size122968-snap-gene-0.23 (protein:Tk09514 transcript:maker-scaffold624_size122968-snap-gene-0.23-mRNA-1 annotation:"ubiquitin-conjugating enzyme e2 g2")

HSP 1 Score: 70.4774 bits (171), Expect = 9.215e-14
Identity = 41/125 (32.80%), Postives = 67/125 (53.60%), Query Frame = 0
Query:  874 LKFVKKEAKLLSSSLPSGIVLKGY-EDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLG------TWSGKGTEIWTPNSNLLQLLLSIQGLI 991
            L+ +  E K LS + P G++     +D    + A I GP DT + DGVF      P DYP +PP    + F S   +PN+Y +G+VC+S+L       T   + +E W+P  ++ ++LLS+  ++
Sbjct:    6 LRRLMSEFKQLSENPPEGLMAGPISDDNYFEWEAAITGPQDTVFEDGVFIARLAFPQDYPLNPPK---MKFMSKIFHPNIYADGRVCISILHAPGDDPTGYEQSSERWSPVQSVEKVLLSVMSML 127          
BLAST of EMLSAG00000000488 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold427_size174323-snap-gene-0.37 (protein:Tk04502 transcript:maker-scaffold427_size174323-snap-gene-0.37-mRNA-1 annotation:"hypothetical protein DAPPUDRAFT_309332")

HSP 1 Score: 61.2326 bits (147), Expect = 5.678e-11
Identity = 35/122 (28.69%), Postives = 60/122 (49.18%), Query Frame = 0
Query:  875 KFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEP 996
            + VK+  +L+   +P GI     +     F  ++ GP  +P+  GVF  +  LP DYP S P    + F +   +PN+ + G++C+ +L        + W+P   +  +LLSIQ L+    P
Sbjct:   14 RIVKETQRLMQEPVP-GISAIPDDGNARYFHVVVAGPEGSPFEGGVFKLELFLPEDYPMSAPK---VRFITKIYHPNIDKLGRICLDIL-------KDKWSPALQIRTVLLSIQALLSAPNP 124          
BLAST of EMLSAG00000000488 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold1280_size50848-snap-gene-0.10 (protein:Tk06598 transcript:maker-scaffold1280_size50848-snap-gene-0.10-mRNA-1 annotation:"ubiquitin-conjugating enzyme")

HSP 1 Score: 62.003 bits (149), Expect = 5.847e-11
Identity = 37/126 (29.37%), Postives = 59/126 (46.83%), Query Frame = 0
Query:  871 KNFLKFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEP 996
            K  LK + KE + L    P+        D +  + A I GP ++P+  GVFF     P DYP  PP    ++F +   +PN+   G +C+ +L +        W+P   + ++LLSI  L+    P
Sbjct:   46 KMALKRINKELQDLGRDPPAQCSAGPVGDDLFHWQATIMGPPESPFQGGVFFLTIHFPTDYPFKPPK---VAFTTRIYHPNINSNGSICLDILRSQ-------WSPALTISKVLLSICSLLCDPNP 161          
BLAST of EMLSAG00000000488 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold658_size117954-snap-gene-0.30 (protein:Tk06732 transcript:maker-scaffold658_size117954-snap-gene-0.30-mRNA-1 annotation:"ubiquitin-conjugating enzyme e2-32 kda")

HSP 1 Score: 61.6178 bits (148), Expect = 2.126e-10
Identity = 36/100 (36.00%), Postives = 52/100 (52.00%), Query Frame = 0
Query:  898 EDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLL-----GTWSGK-GTEIWTPNSNLLQLLLSIQGLI 991
            ED +  +   I GP DT Y  G F    + P DYP SPPT   + F +   +PN+YE G +C+S+L        SG+   E W P  N+  ++LS+  L+
Sbjct:   47 EDNLFEWEMAIFGPPDTLYQGGYFKTQLKFPNDYPYSPPT---VRFLTKVWHPNVYENGDLCISILHPPVDDPQSGELPCERWNPTQNVRTIMLSVVSLL 143          
BLAST of EMLSAG00000000488 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold1057_size73593-snap-gene-0.30 (protein:Tk01341 transcript:maker-scaffold1057_size73593-snap-gene-0.30-mRNA-1 annotation:"ubiquitin-conjugating enzyme e2-32 kda")

HSP 1 Score: 61.6178 bits (148), Expect = 2.126e-10
Identity = 36/100 (36.00%), Postives = 52/100 (52.00%), Query Frame = 0
Query:  898 EDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLL-----GTWSGK-GTEIWTPNSNLLQLLLSIQGLI 991
            ED +  +   I GP DT Y  G F    + P DYP SPPT   + F +   +PN+YE G +C+S+L        SG+   E W P  N+  ++LS+  L+
Sbjct:   47 EDNLFEWEMAIFGPPDTLYQGGYFKTQLKFPNDYPYSPPT---VRFLTKVWHPNVYENGDLCISILHPPVDDPQSGELPCERWNPTQNVRTIMLSVVSLL 143          
BLAST of EMLSAG00000000488 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold1282_size50771-snap-gene-0.19 (protein:Tk06945 transcript:maker-scaffold1282_size50771-snap-gene-0.19-mRNA-1 annotation:"ubiquitin-conjugating enzyme e2 l3")

HSP 1 Score: 52.7582 bits (125), Expect = 8.493e-8
Identity = 35/118 (29.66%), Postives = 56/118 (47.46%), Query Frame = 0
Query:  911 PSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAG-------YESQKFTKHGEENSWMYNE 1021
            P   PY  G F  D Q P +YP  PP    ISF +   +PN+ E+G+VC+ ++        E W P +   Q++ ++  LI   EP     G        + +KF K+ E+ +  ++E
Sbjct:   91 PDSPPYSKGAFKIDIQFPAEYPFKPPK---ISFKTRIYHPNVDEKGQVCLPIV------SAENWKPATKTDQVIQALVSLINEPEPDHPLRGDLAEEYLKDRKKFMKNAEDFTKKHSE 199          
BLAST of EMLSAG00000000488 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold292_size219010-snap-gene-1.30 (protein:Tk00319 transcript:maker-scaffold292_size219010-snap-gene-1.30-mRNA-1 annotation:"GI17815")

HSP 1 Score: 52.7582 bits (125), Expect = 8.738e-8
Identity = 36/141 (25.53%), Postives = 66/141 (46.81%), Query Frame = 0
Query:  875 KFVKKEAKLLSSSLPSGIVLKGYEDRIDLFSAMIKGPSDTPYHDGVFFFDFQLPPDYPKSPPTCFYISFCSDRLNPNLYEEGKVCVSLLGTWSGKGTEIWTPNSNLLQLLLSIQGLILVKEPYFNEAGYESQKFTKHGEEN 1015
            K ++KE   ++   P         D I  + + I GP  + Y  GVFF D    P+YP  PP    ++F +   + N+  +G +C+ +L        + W+P   + ++LLS+  L+    P     G  + ++T++ EE+
Sbjct:   63 KRIQKELAEITLDPPPNCSAGPKGDNIYEWVSTILGPPGSVYEGGVFFLDIHFSPEYPFKPPK---VTFRTRIYHCNINSQGVICLDIL-------KDNWSPALTVSKVLLSVCSLLTDCNPADPLVGSIATQYTQNREEH 193          
The following BLAST results are available for this feature:
BLAST of EMLSAG00000000488 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO)
Total hits: 25
Match NameE-valueIdentityDescription
-5.455e-8149.25symbol:CG10254 species:7227 "Drosophila melanogast... [more]
-4.008e-7455.31symbol:UBE2O "Ubiquitin-conjugating enzyme E2 O" s... [more]
-6.360e-7350.57symbol:Ube2o "ubiquitin-conjugating enzyme E2O" sp... [more]
-1.330e-7250.76symbol:Ube2o "Protein Ube2o" species:10116 "Rattus... [more]
-1.330e-7250.76symbol:Ube2o "ubiquitin-conjugating enzyme E2O" sp... [more]
-1.444e-7255.31symbol:UBE2O "Uncharacterized protein" species:961... [more]
-2.536e-7255.31symbol:UBE2O "Ubiquitin-conjugating enzyme E2 O" s... [more]
-3.424e-7255.31symbol:UBE2O "Uncharacterized protein" species:991... [more]
-3.704e-7255.31symbol:UBE2O "Uncharacterized protein" species:982... [more]
-8.438e-7256.00symbol:UBE2O "Uncharacterized protein" species:903... [more]

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BLAST of EMLSAG00000000488 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA)
Total hits: 25
Match NameE-valueIdentityDescription
gi|592938347|gb|GAXK01020206.1|2.917e-13140.86TSA: Calanus finmarchicus comp12403_c1_seq1 transc... [more]
gi|592938346|gb|GAXK01020207.1|8.109e-12742.50TSA: Calanus finmarchicus comp12403_c1_seq2 transc... [more]
gi|592938335|gb|GAXK01020218.1|8.522e-10037.39TSA: Calanus finmarchicus comp12403_c3_seq1 transc... [more]
gi|592938343|gb|GAXK01020210.1|9.863e-8859.39TSA: Calanus finmarchicus comp12403_c1_seq5 transc... [more]
gi|592938334|gb|GAXK01020219.1|1.567e-8635.71TSA: Calanus finmarchicus comp12403_c3_seq2 transc... [more]
gi|592938341|gb|GAXK01020212.1|2.662e-8369.46TSA: Calanus finmarchicus comp12403_c1_seq7 transc... [more]
gi|592938333|gb|GAXK01020220.1|2.840e-7839.50TSA: Calanus finmarchicus comp12403_c3_seq3 transc... [more]
gi|592938326|gb|GAXK01020227.1|1.054e-7562.56TSA: Calanus finmarchicus comp12403_c3_seq10 trans... [more]
gi|592938340|gb|GAXK01020213.1|2.897e-7362.50TSA: Calanus finmarchicus comp12403_c1_seq8 transc... [more]
gi|592938339|gb|GAXK01020214.1|1.199e-7162.74TSA: Calanus finmarchicus comp12403_c1_seq9 transc... [more]

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BLAST of EMLSAG00000000488 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self)
Total hits: 10
Match NameE-valueIdentityDescription
EMLSAP000000004880.000e+0100.00pep:novel supercontig:LSalAtl2s:LSalAtl2s1075:3670... [more]
EMLSAP000000014971.575e-1329.14pep:novel supercontig:LSalAtl2s:LSalAtl2s1257:2789... [more]
EMLSAP000000087551.927e-1328.48pep:novel supercontig:LSalAtl2s:LSalAtl2s543:24795... [more]
EMLSAP000000071545.323e-1333.60pep:novel supercontig:LSalAtl2s:LSalAtl2s403:12825... [more]
EMLSAP000000070769.507e-1230.89pep:novel supercontig:LSalAtl2s:LSalAtl2s3:487621:... [more]
EMLSAP000000068007.624e-1129.51pep:novel supercontig:LSalAtl2s:LSalAtl2s380:82204... [more]
EMLSAP000000030043.137e-930.58pep:novel supercontig:LSalAtl2s:LSalAtl2s170:74318... [more]
EMLSAP000000065217.314e-939.77pep:novel supercontig:LSalAtl2s:LSalAtl2s357:29932... [more]
EMLSAP000000049727.693e-830.00pep:novel supercontig:LSalAtl2s:LSalAtl2s260:13928... [more]
EMLSAP000000130793.262e-726.56pep:novel supercontig:LSalAtl2s:LSalAtl2s9:891889:... [more]
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BLAST of EMLSAG00000000488 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
gi|342187119|sp|Q6ZPJ3.3|UBE2O_MOUSE5.358e-7350.57RecName: Full=(E3-independent) E2 ubiquitin-conjug... [more]
gi|209572710|sp|Q9C0C9.3|UBE2O_HUMAN2.108e-7255.31RecName: Full=(E3-independent) E2 ubiquitin-conjug... [more]
gi|75315951|sp|Q9ZVX1.1|UBC23_ARATH2.114e-5148.26RecName: Full=Probable ubiquitin-conjugating enzym... [more]
gi|678021173|sp|Q9C918.2|UBC38_ARATH6.380e-4445.26RecName: Full=Putative ubiquitin-conjugating enzym... [more]
gi|205828971|sp|Q8GY87.2|UBC26_ARATH3.056e-4346.32RecName: Full=Probable ubiquitin-conjugating enzym... [more]
gi|75311600|sp|Q9LUQ5.1|UBC25_ARATH4.311e-4347.51RecName: Full=Probable ubiquitin-conjugating enzym... [more]
gi|678019143|sp|F4HPP7.1|UBC39_ARATH3.166e-4150.31RecName: Full=Putative ubiquitin-conjugating enzym... [more]
gi|75331117|sp|Q8VY10.1|UBC24_ARATH1.216e-4045.18RecName: Full=Probable ubiquitin-conjugating enzym... [more]
gi|408360245|sp|Q11076.3|UBC17_CAEEL9.101e-3736.13RecName: Full=Probable ubiquitin-conjugating enzym... [more]
gi|380876899|sp|O88738.2|BIRC6_MOUSE3.245e-3441.53RecName: Full=Baculoviral IAP repeat-containing pr... [more]

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BLAST of EMLSAG00000000488 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods)
Total hits: 25
Match NameE-valueIdentityDescription
gb|KFM57180.1|0.000e+035.63Ubiquitin-conjugating enzyme E2 O, partial [Stegod... [more]
gb|EFA05319.1|2.723e-15231.99hypothetical protein TcasGA2_TC015477 [Tribolium c... [more]
EGK96947.14.061e-11433.25AGAP003090-PB [Anopheles gambiae str. PEST][more]
EAA08452.54.061e-11433.25AGAP003090-PA [Anopheles gambiae str. PEST][more]
XP_006557525.11.335e-9957.14PREDICTED: (E3-independent) E2 ubiquitin-conjugati... [more]
XP_006557524.11.335e-9957.14PREDICTED: (E3-independent) E2 ubiquitin-conjugati... [more]
XP_001120701.21.335e-9957.14PREDICTED: (E3-independent) E2 ubiquitin-conjugati... [more]
EEB14790.18.923e-9742.09ubiquitin-conjugating enzyme E2-230k, putative [Pe... [more]
AAN13952.11.801e-8149.25uncharacterized protein Dmel_CG10254, isoform B [D... [more]
AAF56162.11.801e-8149.25uncharacterized protein Dmel_CG10254, isoform A [D... [more]

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BLAST of EMLSAG00000000488 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017))
Total hits: 25
Match NameE-valueIdentityDescription
gi|939632916|ref|XP_014286335.1|0.000e+038.98PREDICTED: E2/E3 hybrid ubiquitin-protein ligase U... [more]
gi|675364278|gb|KFM57180.1|0.000e+035.63Ubiquitin-conjugating enzyme E2 O, partial [Stegod... [more]
gi|1073701723|gb|JAT93918.1|0.000e+035.28putative ubiquitin-conjugating enzyme e2 o, partia... [more]
gi|939238058|ref|XP_014261696.1|5.179e-5440.80PREDICTED: E2/E3 hybrid ubiquitin-protein ligase U... [more]
gi|915662737|gb|KOC65142.1|1.499e-17743.07Ubiquitin-conjugating enzyme E2 O [Habropoda labor... [more]
gi|357617902|gb|EHJ71057.1|4.620e-16938.51hypothetical protein KGM_13479 [Danaus plexippus][more]
gi|913300714|ref|XP_013199731.1|5.323e-16438.53PREDICTED: E2/E3 hybrid ubiquitin-protein ligase U... [more]
gi|1080049426|ref|XP_018566113.1|2.024e-15231.81PREDICTED: (E3-independent) E2 ubiquitin-conjugati... [more]
gi|91084477|ref|XP_971283.1|1.332e-14931.99PREDICTED: uncharacterized protein LOC659924 [Trib... [more]
gi|817068940|ref|XP_012256424.1|1.645e-14437.08PREDICTED: E2/E3 hybrid ubiquitin-protein ligase U... [more]

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BLAST of EMLSAG00000000488 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins)
Total hits: 9
Match NameE-valueIdentityDescription
maker-scaffold703_size109190-snap-gene-0.183.086e-13837.16protein:Tk04144 transcript:maker-scaffold703_size1... [more]
maker-scaffold865_size87005-snap-gene-0.244.498e-1529.61protein:Tk05467 transcript:maker-scaffold865_size8... [more]
maker-scaffold624_size122968-snap-gene-0.239.215e-1432.80protein:Tk09514 transcript:maker-scaffold624_size1... [more]
maker-scaffold427_size174323-snap-gene-0.375.678e-1128.69protein:Tk04502 transcript:maker-scaffold427_size1... [more]
maker-scaffold1280_size50848-snap-gene-0.105.847e-1129.37protein:Tk06598 transcript:maker-scaffold1280_size... [more]
maker-scaffold658_size117954-snap-gene-0.302.126e-1036.00protein:Tk06732 transcript:maker-scaffold658_size1... [more]
maker-scaffold1057_size73593-snap-gene-0.302.126e-1036.00protein:Tk01341 transcript:maker-scaffold1057_size... [more]
maker-scaffold1282_size50771-snap-gene-0.198.493e-829.66protein:Tk06945 transcript:maker-scaffold1282_size... [more]
maker-scaffold292_size219010-snap-gene-1.308.738e-825.53protein:Tk00319 transcript:maker-scaffold292_size2... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
LSalAtl2s1075supercontigLSalAtl2s1075:36709..41130 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
ensembl2013-09-26 .965016
Blast vs. GO2014-04-02
TblastN vs C. finmarchicus TSA2014-05-09
Blastp vs. self2014-05-10
Blastp vs. SwissProt2017-02-10
Blastp vs. Selected Arthropods2017-02-20
Blastp vs. NR (2/2017)2017-02-20
Blastp vs. Tigriopus kingsejongensis proteins2018-04-18
Properties
Property NameValue
Logic nameensemblgenomes
Descriptionmaker-LSalAtl2s1075-augustus-gene-0.30
Biotypeprotein_coding
EvidenceIEA
Note(E3-independent) E2 ubiquitin-conjugating enzyme UBE2O
Cross References
External references for this gene
DatabaseAccession
Ensembl Metazoa (gene)EMLSAG00000000488 (primary cross-reference)
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
EMLSAT00000000488EMLSAT00000000488-696335Lepeophtheirus salmonismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at LSalAtl2s1075:36709..41130-

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>EMLSAG00000000488-683254 ID=EMLSAG00000000488-683254|Name=EMLSAG00000000488|organism=Lepeophtheirus salmonis|type=gene|length=4422bp|location=Sequence derived from alignment at LSalAtl2s1075:36709..41130- (Lepeophtheirus salmonis)
ATGAGTAGAGGAAGGGAAGAGGAGATTGCACAACTGTTCCATGAGGATGA GGTCTATCATCTGGATAAGAAAGGGAACATTGTCTTTGGTACCGTGGTCA GAGATTGCGAAATGTTGAATTTCGATGATTCTGAGGATGAGGACGAGGAT CGAATGCATCTGAAGGAAGGAGAGGTTTTGGTCTCCTGGTATCCCAAGTA TGAGGAAAAAGTGCATCTGAATTCCTCTCTGAAACTAGGTAGAAAGGACC AGTATGAATGAATGCATTGCTCATTTCTTATATCCTTGTAGGAAATCGGA GTCTGATGCCTGGAGATGTGGTTCGAAGAATGGACTCCCTTGGAAAACGA TCCCAAACGGGGTATTGCCGAGAAATCAAAGTAAGGACATCTGCTGCAAT CAAATGCTCCAATGATCGCGTTATTTATGATATCAACTCTGAGGATTTAA AGCCCATTCAGTCCTTTCTGACCAACTCTGCTGTTTGTTTAGGCCCCTGG GTTGGAGTCATTCGGAAAGTCAAATTGAATGTTGCACTTGCTTTTCATGA TGGTATCTCGAGATGTGTTGTGGATGATAATGTCATCTCTACGTTTGATG ATGTGAATGATAAAGAGCAAAAGATCACTTTGTTCAAGAGGTTAGATTAT TATCCAGGCATGATACTCACTGGACCTTTGAGAGAATTTGAAGGAGCAGA GTGGTTCTCTTGTTCTGATAAGTTAAAGGAACTCATGAGTAAAAAACCCA TGAAATTTGTTAAAGTCACAGTTTTAAAGACTCAAGTCCGTGAACTCTCT GTTAATTGGCAATGTCAGGCCTGTGTTGGGAACGAGTCAGATGGAATAGC TCCAGATATCATCCAGGGCGAAGATCTCAAAAGGTACATTTAATTTTAAG TATACAATGTCAATGTCAAAACTCCCGAGTATTTATCTCTTGTATTACTT ATTCATATTCATACTCACGTCTTATTCTTTTGTATTTGACTTCTTTAAAT GTAGAATAATATAATTTCAAAAAGTTTATTTTCTTCTTTAGAACCAAGAT ACTGGGATATTTTTTCCCCTGCACCCTCCAAATCGGTGATCGTTTTTTTT ATAAAATTAAAGAGGAAGACGTTATTTTATCTAAAAGTAAATGGCTCCAA CGTGAGAGAGAGATTTTAGCAAATGAAATGGATGGTGAATCAAAACCCGT GGATTCCATTAAACTTAAAGAATTTGATGTTAAAAAAGAGGAAGCAGAAT CAAAGTCACTCTCCGTTCCCAATAACTCCATAAATAAGAGTAATGTCTCT ATCAGTGTGAATAAGTTAAGGAGACGATTGAAAAAAAGGAATAAAAGTGT TACCGCAGAATTATATTCTAATTTACCGAGTTGCTACAGTTCAGTTATAC CTAAAATTCGTCCTGGTGAGATTGTTGTTGTTGAAGCGTTACGCACGAAA GCCGAAGTCTCTGTGCTTTGGCAAGATGGATCTTTAGAAGAGGGAATTGA TTCTAGCGAGTTATACCCCATCCATCACTTAGATGATAAAGAATTCTTTA CTGGTGAATTTGTTGTTCGAGCTTCTGATTTGGGAGGTCTACAAAGTTAT GGAGTTGTACAACACGTGGACTATCAATCAAGAATAGCTAAAATCAAATG GTTTCATACTTATCAGATTGGAAGTGATCCAAGGTATATTATGATTTGTT TTTATATTACTTAATGGATTTCAAATGTGTTTGTATGTATTTTTCTAGCC CGTCGTATGTTGGAGAGTCTGAAGAGTCAGTTTATGATCTAAGAGACCAT CCGGATTTTAATTATAGGCCTGGTTCTGTAGTAATCAAGGTAGCAAATTT TGATAATGAAGAATCTGAAGGTGGTTTGGGTGGTGAAGTTATAGAGGTTT ATCCTAATGGCAAAGTGACTGTGTGGTGGGCCAACAAAAAAACCACTGAA TGTTGGCCACAGGATTTATATAAAGTTGGAGAATACGACTCTGATGAGGG TGAACTTTGGGATGATGATTCCGACTTTGAAGGAGAAGAGAAAGATGAGG TTGAAGAGGATTCTTGGGTAATGCTAACTAAATTGGTTGGTTCTGGAGAA TCGGAAAAAACTAGGTAATTCTGACGATATCTTTTTTTTTTTTAATATAG TTTTTTTTTCTTGCTTTTTTAAGTGAATCTATCGTATCTGAAAAATGTCC TAGAGTAATAGTGTTTGGACTATTTGAAAAAATAAGGACTTCGCTACAAA AACTCGAAGAAAATTTCTCTGAGGCCTCAAAACCTCAAACGTCAGCTATG TTAGAGAAGCTATGGGAAATATATAAAGACTGTTGTGTTCTAGAAAAGTC TATAGGTACTTCATATTTTGCATTGGAAAAATTTGCAAATATTATAGATC GTACTTCTGGAAAATTAAATAAAGTGGCTGGAAATAAATTGGCTGTTCAA GATCAGATAAGCCGTTTATTTAATAGTACCAGTGAATTTCAAAATATAAA TGCCAAAGAAAATTCCCTTCCATCTAATGACGAACAAGATATATTTTATG AGGTAAGGCAAGCTATGAGATATCGGTGTGAAGAGTCTGGATGTACATGC ATATAGTTATGTTCTTTAATTCGGGGTTGATATATTTTACTTTTAGAATC TGTTAGCACTAATTATTTAGGGGAAAATACTTCATTTATCATATTTTCTT GTAGAACACGTGTCGTCGGATATGCTCATTATTCAAGTTAAAGTTAGCGT ATGTTTATGATGTTATAGTTGAAAAGTATGGGACAATGGTACCCCAACTT TCTATACTAGAAGAAATACAACATGATAAGGATCTCGATATATTGCTTTC AATTACTGGACTGACCTATGATGACATTTCCCCAGTTCTTCCCAAAGAAG AAGGAGAACATAAAATTGAGGCACCTCAAGTTGAAAAAGTCGTAGTTTCT CCTCCTGAAGAAGTGTTGTCTCCTGTTGAACTTCAATCTCTTCCTTTAGA AGCTGTACTGAATTTCAAAAATGGTACATTTAAAATTGAGGAAAGTGCTC CACAGGAGCATACTTTTAAAGTTTCGCTGTTTGAACCTGTAGACCCTAAA AATTTCCTCAAGTTTGTTAAAAAAGAAGCAAAATTACTTTCAAGTAGTCT ACCATCTGGTATTGTTCTGAAAGGATATGAAGACCGCATAGACTTATTCT CTGCCATGATAAAAGGTCCTTCAGATACACCTTACCACGATGGTGTATTC TTTTTCGACTTTCAACTACCTCCCGATTATCCTAAGTCTCCTCCTACGTG CTTCTATATTAGTTTCTGCAGTGATCGCTTGAACCCAAATTTGTATGAAG AGGGTAAAGTGTGTGTATCACTTCTTGGAACATGGAGTGGCAAAGGTACT GAAATATGGACACCTAACTCAAATCTTCTTCAGCTCCTTCTATCAATACA AGGTGTGCTTTTATTCATATTTTTTTCATCAGAGATTTTCAATCGTTTAA GCTATTTAAATTAAAAGGAGTAGTAACTTTATGTACCTTAGAAAAATATC GTAACTTGATTATTGTTGCGTTAAAACCAATTAAGCTACTTTTTTAACAT TAAACCAACATCGAATACAAGTTTTAAAAGTAATCCTATTGATTTAGTCA CACTCTGTTTGACTTCTATCATATTTTCCTACTGAACACGTTTCGTCTAA TATACTGATTATTCAAGTTAACCTTGGCTTCTATTTATGATCTTATAGTC TGCATTTAGGACAATATTATTCACAATATGTGCAAATCGCCTGCTTCTAC TTTATTACGTTAAAGCGAGGGTTTTTGCCAGTCCTACTGTTCACTTTATA CTAATTTATTATATTAATTAAATAATTTTAAAAATTACTTTTTAAGGGCT TATATTAGTTAAAGAGCCTTACTTTAACGAGGCAGGATATGAAAGTCAGA AGTTCACTAAACATGGAGAAGAAAATTCATGGATGTATAATGAAATGGTA GTAATCAAAATAGTTCAAAGTATGACTCGACTTTTAATTAGTCCTCCTCC CATTTTTAAAGATGAAATTTTAGAACATATTAATGAGCGTGGAGATAAGT AAGTTAATTATAGCAGAAAATATATCTTTTGTAAAATCTGTAATTTTTTT CTTAAAATATAGATTTGTTAAAAGATGGAGAGAATGGCTCSAAATTTCAT CTTCGTATCATTCAAGTAATGTTGAGGGKATTGTATTAATTAATTAAGTG TCCATTCTAGTTTTATTAATGATTGTTTTTTAATTATATTTCAGATAAGA AGGAAATATCTCCTTTAGATTTCCCATTGTTACCAGCTTCTAAAGGATTT TGCGTCTCCTTTGCAAAATCATTGGATGAATTTGATTTAGCGCATTCGAA ATACTTGGCATCAAATAGTTAG
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