EMLSAG00000001066, EMLSAG00000001066-683832 (gene) Lepeophtheirus salmonis

Overview
NameEMLSAG00000001066
Unique NameEMLSAG00000001066-683832
Typegene
OrganismLepeophtheirus salmonis (salmon louse)
Associated RNAi Experiments

Nothing found

Homology
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BLAST of EMLSAG00000001066 vs. GO
Match: - (symbol:bt "bent" species:7227 "Drosophila melanogaster" [GO:0005737 "cytoplasm" evidence=ISS] [GO:0004687 "myosin light chain kinase activity" evidence=NAS] [GO:0005200 "structural constituent of cytoskeleton" evidence=ISS] [GO:0004674 "protein serine/threonine kinase activity" evidence=IEA;NAS] [GO:0006468 "protein phosphorylation" evidence=IEA;NAS] [GO:0007498 "mesoderm development" evidence=IEP] [GO:0005524 "ATP binding" evidence=IEA] [GO:0008307 "structural constituent of muscle" evidence=IDA] [GO:0030018 "Z disc" evidence=IDA] [GO:0045214 "sarcomere organization" evidence=IMP] InterPro:IPR000719 InterPro:IPR002290 InterPro:IPR003961 InterPro:IPR007110 InterPro:IPR008271 InterPro:IPR011009 InterPro:IPR017441 Pfam:PF00041 Pfam:PF00069 PROSITE:PS00107 PROSITE:PS00108 PROSITE:PS50011 PROSITE:PS50835 PROSITE:PS50853 SMART:SM00060 SMART:SM00220 GO:GO:0005524 Gene3D:2.60.40.10 InterPro:IPR013783 GO:GO:0030018 GO:GO:0007498 SUPFAM:SSF49265 SUPFAM:SSF56112 GO:GO:0004674 InterPro:IPR003599 SMART:SM00409 InterPro:IPR003598 SMART:SM00408 GO:GO:0045214 GO:GO:0008307 InterPro:IPR013098 Pfam:PF07679 EMBL:AE014135 OrthoDB:EOG72C4ZC GeneTree:ENSGT00720000108588 GO:GO:0004687 OMA:GCKLKWK RefSeq:NP_001162825.1 UniGene:Dm.4645 ProteinModelPortal:D1YSG0 SMR:D1YSG0 EnsemblMetazoa:FBtr0301340 GeneID:43814 KEGG:dme:Dmel_CG32019 CTD:43814 FlyBase:FBgn0005666 SignaLink:D1YSG0 GenomeRNAi:43814 NextBio:836022 PRO:PR:D1YSG0 Bgee:D1YSG0 Uniprot:D1YSG0)

HSP 1 Score: 42.743 bits (99), Expect = 2.423e-4
Identity = 21/47 (44.68%), Postives = 30/47 (63.83%), Query Frame = 0 
Query:   43 QDTGIYTIRLTCESGTFEASGKVNVLDVPLVP-RSLQPDEVHAEHVK 88
            +DTG Y + L+  SGT E+  +V VLD PL P    +P+E+ A H+K
Sbjct: 2371 KDTGKYKLVLSNSSGTIESEAQVVVLDRPLPPGGPFEPEEIRASHIK 2417
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BLAST of EMLSAG00000001066 vs. C. finmarchicus
Match: gi|592890593|gb|GAXK01067782.1| (TSA: Calanus finmarchicus comp17337_c10_seq3 transcribed RNA sequence)

HSP 1 Score: 34.6538 bits (78), Expect = 2.322e-2
Identity = 18/38 (47.37%), Postives = 24/38 (63.16%), Query Frame = 0 
Query:   38 KADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPR 75
            K  +  DTG Y I+LT   G+ +AS KVNV+D P  P+
Sbjct: 3073 KKAKRTDTGTYNIQLTNTEGSDQASCKVNVVDRPSPPQ 3186
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BLAST of EMLSAG00000001066 vs. C. finmarchicus
Match: gi|592890594|gb|GAXK01067781.1| (TSA: Calanus finmarchicus comp17337_c10_seq2 transcribed RNA sequence)

HSP 1 Score: 34.6538 bits (78), Expect = 2.325e-2
Identity = 18/38 (47.37%), Postives = 24/38 (63.16%), Query Frame = 0 
Query:   38 KADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPR 75
            K  +  DTG Y I+LT   G+ +AS KVNV+D P  P+
Sbjct: 3135 KKAKRTDTGTYNIQLTNTEGSDQASCKVNVVDRPSPPQ 3248
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BLAST of EMLSAG00000001066 vs. C. finmarchicus
Match: gi|592890666|gb|GAXK01067709.1| (TSA: Calanus finmarchicus comp17337_c9_seq6 transcribed RNA sequence)

HSP 1 Score: 34.6538 bits (78), Expect = 2.450e-2
Identity = 18/38 (47.37%), Postives = 24/38 (63.16%), Query Frame = 0 
Query:   38 KADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPR 75
            K  +  DTG Y I+LT   G+ +AS KVNV+D P  P+
Sbjct:  265 KKAKRTDTGTYNIQLTNTEGSDQASCKVNVVDRPSPPQ 378
[Close]
BLAST of EMLSAG00000001066 vs. C. finmarchicus
Match: gi|592890670|gb|GAXK01067705.1| (TSA: Calanus finmarchicus comp17337_c9_seq2 transcribed RNA sequence)

HSP 1 Score: 34.6538 bits (78), Expect = 2.479e-2
Identity = 18/38 (47.37%), Postives = 24/38 (63.16%), Query Frame = 0 
Query:   38 KADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPR 75
            K  +  DTG Y I+LT   G+ +AS KVNV+D P  P+
Sbjct:  265 KKAKRTDTGTYNIQLTNTEGSDQASCKVNVVDRPSPPQ 378
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BLAST of EMLSAG00000001066 vs. C. finmarchicus
Match: gi|592890581|gb|GAXK01067794.1| (TSA: Calanus finmarchicus comp17337_c10_seq15 transcribed RNA sequence)

HSP 1 Score: 31.5722 bits (70), Expect = 2.150e-1
Identity = 19/65 (29.23%), Postives = 33/65 (50.77%), Query Frame = 0 
Query:   12 KLRIQHSETHTGLKSVQNATGSERAYKADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRS 76
            K ++ +S+ H  +   +  T  + A+   +  D G+YT+ ++ E GT  A   V VLD P  P +
Sbjct: 1107 KNKVVYSDEHYDVMVQRRQTVIDIAFS--KRYDAGVYTLEVSNELGTATARANVTVLDRPAPPEA 1295

HSP 2 Score: 27.7202 bits (60), Expect = 4.751e+0
Identity = 14/34 (41.18%), Postives = 19/34 (55.88%), Query Frame = 0 
Query:   41 RDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVP 74
            +  DTG   I+L  ESG+ +     N+LDVP  P
Sbjct:  231 KRSDTGDIVIKLVNESGSDKGGFYFNILDVPGPP 332
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BLAST of EMLSAG00000001066 vs. C. finmarchicus
Match: gi|592890576|gb|GAXK01067799.1| (TSA: Calanus finmarchicus comp17337_c10_seq20 transcribed RNA sequence)

HSP 1 Score: 31.5722 bits (70), Expect = 2.179e-1
Identity = 19/65 (29.23%), Postives = 33/65 (50.77%), Query Frame = 0 
Query:   12 KLRIQHSETHTGLKSVQNATGSERAYKADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRS 76
            K ++ +S+ H  +   +  T  + A+   +  D G+YT+ ++ E GT  A   V VLD P  P +
Sbjct:  904 KNKVVYSDEHYDVMVQRRQTVIDIAFS--KRYDAGVYTLEVSNELGTATARANVTVLDRPAPPEA 1092

HSP 2 Score: 27.7202 bits (60), Expect = 4.946e+0
Identity = 14/34 (41.18%), Postives = 19/34 (55.88%), Query Frame = 0 
Query:   41 RDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVP 74
            +  DTG   I+L  ESG+ +     N+LDVP  P
Sbjct:   28 KRSDTGDIVIKLVNESGSDKGGFYFNILDVPGPP 129
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BLAST of EMLSAG00000001066 vs. C. finmarchicus
Match: gi|592890648|gb|GAXK01067727.1| (TSA: Calanus finmarchicus comp17337_c9_seq24 transcribed RNA sequence)

HSP 1 Score: 31.5722 bits (70), Expect = 2.377e-1
Identity = 19/65 (29.23%), Postives = 33/65 (50.77%), Query Frame = 0 
Query:   12 KLRIQHSETHTGLKSVQNATGSERAYKADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRS 76
            K ++ +S+ H  +   +  T  + A+   +  D G+YT+ ++ E GT  A   V VLD P  P +
Sbjct:  466 KNKVVYSDEHYDVMVQRRQTVIDIAFS--KRYDAGVYTLEVSNELGTATARANVTVLDRPAPPEA 654

HSP 2 Score: 27.7202 bits (60), Expect = 5.185e+0
Identity = 14/34 (41.18%), Postives = 19/34 (55.88%), Query Frame = 0 
Query:   41 RDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVP 74
            +  DTG   I+L  ESG+ +     N+LDVP  P
Sbjct: 1429 KRSDTGDIVIKLVNESGSDKGGFYFNILDVPGPP 1530
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BLAST of EMLSAG00000001066 vs. C. finmarchicus
Match: gi|592890651|gb|GAXK01067724.1| (TSA: Calanus finmarchicus comp17337_c9_seq21 transcribed RNA sequence)

HSP 1 Score: 31.187 bits (69), Expect = 3.095e-1
Identity = 19/65 (29.23%), Postives = 33/65 (50.77%), Query Frame = 0 
Query:   12 KLRIQHSETHTGLKSVQNATGSERAYKADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRS 76
            K ++ +S+ H  +   +  T  + A+   +  D G+YT+ ++ E GT  A   V VLD P  P +
Sbjct:  926 KNKVVYSDEHYDVMVQRRQTVIDIAFS--KRYDAGVYTLEVSNELGTATARANVTVLDRPAPPEA 1114

HSP 2 Score: 29.261 bits (64), Expect = 1.606e+0
Identity = 15/48 (31.25%), Postives = 27/48 (56.25%), Query Frame = 0 
Query:   41 RDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRS-LQPDEVHAEHV 87
            +  DTG+Y +    ++GT   +  V VLDVP  P + + P E+  +++
Sbjct:  122 KRSDTGVYKLVAKNKNGTDTMTCNVTVLDVPGPPEAPVDPKEIRKDYM 265

HSP 3 Score: 27.7202 bits (60), Expect = 6.156e+0
Identity = 14/34 (41.18%), Postives = 19/34 (55.88%), Query Frame = 0 
Query:   41 RDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVP 74
            +  DTG   I+L  ESG+ +     N+LDVP  P
Sbjct: 1889 KRSDTGDIVIKLVNESGSDKGGFYFNILDVPGPP 1990
[Close]
BLAST of EMLSAG00000001066 vs. C. finmarchicus
Match: gi|592890661|gb|GAXK01067714.1| (TSA: Calanus finmarchicus comp17337_c9_seq11 transcribed RNA sequence)

HSP 1 Score: 31.187 bits (69), Expect = 3.255e-1
Identity = 19/65 (29.23%), Postives = 33/65 (50.77%), Query Frame = 0 
Query:   12 KLRIQHSETHTGLKSVQNATGSERAYKADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRS 76
            K ++ +S+ H  +   +  T  + A+   +  D G+YT+ ++ E GT  A   V VLD P  P +
Sbjct: 2441 KNKVVYSDEHYDVMVQRRQTVIDIAFS--KRYDAGVYTLEVSNELGTATARANVTVLDRPAPPEA 2629

HSP 2 Score: 29.261 bits (64), Expect = 1.677e+0
Identity = 15/48 (31.25%), Postives = 27/48 (56.25%), Query Frame = 0 
Query:   41 RDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRS-LQPDEVHAEHV 87
            +  DTG+Y +    ++GT   +  V VLDVP  P + + P E+  +++
Sbjct: 1637 KRSDTGVYKLVAKNKNGTDTMTCNVTVLDVPGPPEAPVDPKEIRKDYM 1780

HSP 3 Score: 27.7202 bits (60), Expect = 6.412e+0
Identity = 14/34 (41.18%), Postives = 19/34 (55.88%), Query Frame = 0 
Query:   41 RDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVP 74
            +  DTG   I+L  ESG+ +     N+LDVP  P
Sbjct: 3404 KRSDTGDIVIKLVNESGSDKGGFYFNILDVPGPP 3505
[Close]
BLAST of EMLSAG00000001066 vs. C. finmarchicus
Match: gi|592772905|gb|GAXK01181663.1| (TSA: Calanus finmarchicus comp129544_c2_seq1 transcribed RNA sequence)

HSP 1 Score: 30.8018 bits (68), Expect = 4.441e-1
Identity = 14/34 (41.18%), Postives = 20/34 (58.82%), Query Frame = 0 
Query:   38 KADRDQDTGIYTIRLTCESGTFEASGKVNVLDVP 71
            KA  D   G+Y +RL  ESG FE++  V++   P
Sbjct: 3081 KAMEDNHGGVYKLRLKNESGVFESAANVDIDGPP 3182

HSP 2 Score: 28.8758 bits (63), Expect = 1.898e+0
Identity = 14/44 (31.82%), Postives = 24/44 (54.55%), Query Frame = 0 
Query:   44 DTGIYTIRLTCESGTFEASGKVNVLDVPLVPRSLQPDEVHAEHV 87
            ++GIY + ++ + G+ E    V VLD+P  P S+   E   + V
Sbjct: 2157 NSGIYKVTISNDQGSCEIDVPVEVLDIPSPPLSVSVKEFTKDSV 2288
[Close]
BLAST of EMLSAG00000001066 vs. L. salmonis peptides
Match: EMLSAP00000001066 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1177:93902:94514:-1 gene:EMLSAG00000001066 transcript:EMLSAT00000001066 description:"maker-LSalAtl2s1177-snap-gene-0.7")

HSP 1 Score: 184.111 bits (466), Expect = 2.134e-61
Identity = 88/88 (100.00%), Postives = 88/88 (100.00%), Query Frame = 0 
Query:    1 MEGQTSKNTLSKLRIQHSETHTGLKSVQNATGSERAYKADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRSLQPDEVHAEHVK 88
            MEGQTSKNTLSKLRIQHSETHTGLKSVQNATGSERAYKADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRSLQPDEVHAEHVK
Sbjct:    1 MEGQTSKNTLSKLRIQHSETHTGLKSVQNATGSERAYKADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRSLQPDEVHAEHVK 88
[Close]
BLAST of EMLSAG00000001066 vs. L. salmonis peptides
Match: EMLSAP00000000141 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1021:93859:109614:1 gene:EMLSAG00000000141 transcript:EMLSAT00000000141 description:"maker-LSalAtl2s1021-augustus-gene-0.30")

HSP 1 Score: 68.9366 bits (167), Expect = 9.702e-15
Identity = 31/50 (62.00%), Postives = 38/50 (76.00%), Query Frame = 0 
Query:   38 KADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRSLQPDEVHAEHV 87
            KA+R  DTG YTI+L C+ GT +A+G VNVLDVP  PR L PDE+ AEH+
Sbjct:  897 KANRSIDTGHYTIKLLCDGGTQQATGYVNVLDVPEKPRXLNPDEIRAEHI 946
[Close]
BLAST of EMLSAG00000001066 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold17_size721972-snap-gene-1.18 (protein:Tk09953 transcript:maker-scaffold17_size721972-snap-gene-1.18-mRNA-1 annotation:"LD10678p")

HSP 1 Score: 80.1073 bits (196), Expect = 1.467e-19
Identity = 37/51 (72.55%), Postives = 44/51 (86.27%), Query Frame = 0 
Query:   38 KADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRSLQPDEVHAEHVK 88
            +ADR +DTG+YTI+LTCE+GTFEASG+VNVLDVP   R L PDEV AEH+K
Sbjct:  925 RADRVRDTGLYTIKLTCEAGTFEASGRVNVLDVPTKCRQLVPDEVRAEHIK 975
[Close]
BLAST of EMLSAG00000001066 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold175_size286436-snap-gene-1.47 (protein:Tk09286 transcript:maker-scaffold175_size286436-snap-gene-1.47-mRNA-1 annotation:"GK13703")

HSP 1 Score: 76.2554 bits (186), Expect = 3.769e-18
Identity = 36/51 (70.59%), Postives = 40/51 (78.43%), Query Frame = 0 
Query:   38 KADRDQDTGIYTIRLTCESGTFEASGKVNVLDVPLVPRSLQPDEVHAEHVK 88
            KA+R  D G Y IRL CE GTFEA+G VNVLDVP  PRSL+PDE+ AEHVK
Sbjct:  426 KAERAVDAGHYKIRLVCEGGTFEATGYVNVLDVPEKPRSLRPDEIRAEHVK 476
The following BLAST results are available for this feature:
BLAST of EMLSAG00000001066 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO)
Total hits: 1
Match NameE-valueIdentityDescription
-2.423e-444.68symbol:bt "bent" species:7227 "Drosophila melanoga... [more]
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BLAST of EMLSAG00000001066 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA)
Total hits: 25
Match NameE-valueIdentityDescription
gi|592890593|gb|GAXK01067782.1|2.322e-247.37TSA: Calanus finmarchicus comp17337_c10_seq3 trans... [more]
gi|592890594|gb|GAXK01067781.1|2.325e-247.37TSA: Calanus finmarchicus comp17337_c10_seq2 trans... [more]
gi|592890666|gb|GAXK01067709.1|2.450e-247.37TSA: Calanus finmarchicus comp17337_c9_seq6 transc... [more]
gi|592890670|gb|GAXK01067705.1|2.479e-247.37TSA: Calanus finmarchicus comp17337_c9_seq2 transc... [more]
gi|592890581|gb|GAXK01067794.1|2.150e-129.23TSA: Calanus finmarchicus comp17337_c10_seq15 tran... [more]
gi|592890576|gb|GAXK01067799.1|2.179e-129.23TSA: Calanus finmarchicus comp17337_c10_seq20 tran... [more]
gi|592890648|gb|GAXK01067727.1|2.377e-129.23TSA: Calanus finmarchicus comp17337_c9_seq24 trans... [more]
gi|592890651|gb|GAXK01067724.1|3.095e-129.23TSA: Calanus finmarchicus comp17337_c9_seq21 trans... [more]
gi|592890661|gb|GAXK01067714.1|3.255e-129.23TSA: Calanus finmarchicus comp17337_c9_seq11 trans... [more]
gi|592772905|gb|GAXK01181663.1|4.441e-141.18TSA: Calanus finmarchicus comp129544_c2_seq1 trans... [more]

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BLAST of EMLSAG00000001066 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self)
Total hits: 2
Match NameE-valueIdentityDescription
EMLSAP000000010662.134e-61100.00pep:novel supercontig:LSalAtl2s:LSalAtl2s1177:9390... [more]
EMLSAP000000001419.702e-1562.00pep:novel supercontig:LSalAtl2s:LSalAtl2s1021:9385... [more]
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BLAST of EMLSAG00000001066 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000001066 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000001066 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017))
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000001066 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins)
Total hits: 2
Match NameE-valueIdentityDescription
maker-scaffold17_size721972-snap-gene-1.181.467e-1972.55protein:Tk09953 transcript:maker-scaffold17_size72... [more]
maker-scaffold175_size286436-snap-gene-1.473.769e-1870.59protein:Tk09286 transcript:maker-scaffold175_size2... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
LSalAtl2s1177supercontigLSalAtl2s1177:93902..94514 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
ensembl2013-09-26 .965016
Blast vs. GO2014-04-02
TblastN vs C. finmarchicus TSA2014-05-09
Blastp vs. self2014-05-10
Blastp vs. SwissProt2017-02-10
Blastp vs. Selected Arthropods2017-02-20
Blastp vs. NR (2/2017)2017-02-20
Blastp vs. Tigriopus kingsejongensis proteins2018-04-18
Properties
Property NameValue
Logic nameensemblgenomes
Descriptionmaker-LSalAtl2s1177-snap-gene-0.7
Biotypeprotein_coding
EvidenceIEA
NoteHypothetical protein
Cross References
External references for this gene
DatabaseAccession
Ensembl Metazoa (gene)EMLSAG00000001066 (primary cross-reference)
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
EMLSAT00000001066EMLSAT00000001066-696913Lepeophtheirus salmonismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at LSalAtl2s1177:93902..94514-

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>EMLSAG00000001066-683832 ID=EMLSAG00000001066-683832|Name=EMLSAG00000001066|organism=Lepeophtheirus salmonis|type=gene|length=613bp|location=Sequence derived from alignment at LSalAtl2s1177:93902..94514- (Lepeophtheirus salmonis)
ATGGARGGACAGACGTCAAAAAATACATTATCGAAGCTTCGGATACAACA
TTCGGAAACTCACACTGGACTAAAGTCGGTACAAAATGCCACGGGATCAG
AAAGAGCTTATAAGGTGGATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGGT
CGTCCKACCCCAACAGATAAAGACACATCTATTGAATTATACAGCAAAAA
CGGTATCTATACCGAGAAAAATACCGTTTTGAGTATACKCAGGGCATGCA
TCCTCAGAACATATTCATCCTCATTTATAAATGATGAACTATATATATAT
ATATTTCCAGGCTGATAGAGACCAGGACACTGGAATATATACAATTCGTT
TAACTTGTGAATCTGGTACTTTTGAGGCCTCAGGAAAAGTCAATGTCTTG
GATGTGCCTCTAGTACCCAGGTCTCTCCAACCCGATGAGGTCCATGCAGA
ACATGTCAAATAA
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