EMLSAG00000002599, EMLSAG00000002599-685365 (gene) Lepeophtheirus salmonis

Overview
NameEMLSAG00000002599
Unique NameEMLSAG00000002599-685365
Typegene
OrganismLepeophtheirus salmonis (salmon louse)
Associated RNAi Experiments

Nothing found

Homology
BLAST of EMLSAG00000002599 vs. GO
Match: - (symbol:TSC2 "Tuberin" species:9606 "Homo sapiens" [GO:0005096 "GTPase activator activity" evidence=IEA] [GO:0005634 "nucleus" evidence=IEA] [GO:0005737 "cytoplasm" evidence=IDA] [GO:0032007 "negative regulation of TOR signaling" evidence=IEA] [GO:0033596 "TSC1-TSC2 complex" evidence=IEA] [GO:0051056 "regulation of small GTPase mediated signal transduction" evidence=IEA] InterPro:IPR000331 InterPro:IPR003913 InterPro:IPR016024 InterPro:IPR018515 InterPro:IPR027107 Pfam:PF02145 Pfam:PF03542 PRINTS:PR01431 PROSITE:PS50085 GO:GO:0005634 SUPFAM:SSF48371 Gene3D:1.25.10.10 InterPro:IPR011989 GO:GO:0005096 GO:GO:0051056 GO:GO:0032007 EMBL:AC093513 PANTHER:PTHR10063 GO:GO:0033596 HGNC:HGNC:12363 InterPro:IPR024584 PANTHER:PTHR10063:SF1 Pfam:PF11864 ProteinModelPortal:H3BMQ0 Ensembl:ENST00000568454 NextBio:35525605 ArrayExpress:H3BMQ0 Bgee:H3BMQ0 Uniprot:H3BMQ0)

HSP 1 Score: 489.96 bits (1260), Expect = 2.568e-143
Identity = 307/995 (30.85%), Postives = 511/995 (51.36%), Query Frame = 0
Query:   50 LQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIH------------------------AAKSKWLNI--FSELVERYYKKEKRVN-----------------IRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAER--DSLLK-----------------------DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACL-----LVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSA-ARRPNVALAAKKDVAADVPKXTQIHPLA--FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTML 967
            L  R++++  + E  +  + ++  VE L     DLL+ + P + RH VL+  +++V GQ  RLG++R+ FF +IK+  S     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YLD + +  ++ +I  LC  T    + +  L+VL++++CY+ +P   L LFI  LCR +N++   +  W + R L+GTHLGHSA+Y +  +++      D  L+RGA+FF+G +LWG +R+ SL  S  ++LP+F  A+ C + +V +E++  + +L+  Y KEL   A D ++ +IE LL +++ L+      + + +H ++T +E L +   + GS+++ F +++ CAD RPE S++ LI++R +SIH                        A + K L++  F  L+ R + +E+ +N                 +R     +++D+ +  H      L+D  II  + +  + P   +  R +   +      +T   L LL IL   +       A R  + L+                        D L+++  D  + LGL   DG   FSPY +C   E E  +E       +  S P SP         +   V L          ++++CL++E+DW VL LVL R+P  L+ + LI     S+++  + L S           L   P  FS++D H  + PVL AL SY+ +L    Q  ++  LE GL+ + C   C+VAL+  ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV FI   L SN+ + F++  + KDS  R RSTSL+   +R   +L +    +AD     Q        H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L
Sbjct:   65 LNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNE---DLHERLEVFKALTDNGRHITYLEEELADFVLQWM-----DVGLSSEFLLVLVNLVKFNSCYLD-EYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSP---ELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLD--IIEKVMARSLSPPPELEERDVAAYSASLEDVKT-AVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSE-------KKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLE-RLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHR-CASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPE-KDSF-RARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKL 1033          

HSP 2 Score: 243.432 bits (620), Expect = 2.003e-63
Identity = 130/313 (41.53%), Postives = 189/313 (60.38%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKIT-ENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELK----EPLGHVMS---LSILVRQWALHCNLAAQIQKSLSNKMP-YSSNWLZXLRAIKRLKEKLQKE 1534
            R +TIS S+P  R            V R  +    +    +KV GI+P FVFL LY S  FG+ ++  KP+LLP+   + E S++ LD+IP+++THKI VLY+G  Q N E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ +FH+ATLMP  D D     KKRH+GN++ +IVYNDS    E +K+GT++G+F    +++TPLD   N V + C+ +++      +  ++S   L  + RQ ALH N+A+Q+  S SN    Y S W+  LR IKRL++++ +E
Sbjct: 1403 RGYTISDSAPSRR---------GKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESN--KPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDS---GEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEE 1701          
BLAST of EMLSAG00000002599 vs. GO
Match: - (symbol:TSC2 "Tuberin" species:9606 "Homo sapiens" [GO:0005096 "GTPase activator activity" evidence=IEA] [GO:0005634 "nucleus" evidence=IEA] [GO:0005737 "cytoplasm" evidence=IDA] [GO:0032007 "negative regulation of TOR signaling" evidence=IEA] [GO:0033596 "TSC1-TSC2 complex" evidence=IEA] [GO:0051056 "regulation of small GTPase mediated signal transduction" evidence=IEA] InterPro:IPR000331 InterPro:IPR003913 InterPro:IPR016024 InterPro:IPR018515 InterPro:IPR027107 Pfam:PF02145 Pfam:PF03542 PRINTS:PR01431 PROSITE:PS50085 GO:GO:0005634 GO:GO:0005737 SUPFAM:SSF48371 Gene3D:1.25.10.10 InterPro:IPR011989 GO:GO:0005096 GO:GO:0051056 GO:GO:0032007 EMBL:AC093513 PANTHER:PTHR10063 GO:GO:0033596 UniGene:Hs.90303 HGNC:HGNC:12363 HOVERGEN:HBG018005 InterPro:IPR024584 PANTHER:PTHR10063:SF1 Pfam:PF11864 HOGENOM:HOG000045987 EMBL:AK295672 ProteinModelPortal:B4DIL8 Ensembl:ENST00000382538 UCSC:uc010uvw.2 NextBio:35472310 ArrayExpress:B4DIL8 Uniprot:B4DIL8)

HSP 1 Score: 488.034 bits (1255), Expect = 5.216e-143
Identity = 307/996 (30.82%), Postives = 510/996 (51.20%), Query Frame = 0
Query:   50 LQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIH------------------------AAKSKWLNI--FSELVERYYKKEKRVN-----------------IRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAER--DSLLK-----------------------DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACL-----LVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLS--AARRPNVALAAKKDVAADVPKXTQIHPLA--FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTML 967
            L  R++++  + E  +  + ++  VE L     DLL+ + P + RH VL+  +++V GQ  RLG++R+ FF +IK+  S     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YLD + +  ++ +I  LC  T    + +  L+VL++++CY+ +P   L LFI  LCR +N++   +  W + R L+GTHLGHSA+Y +  +++      D  L+RGA+FF+G +LWG +R+ SL  S  ++LP+F  A+ C + +V +E++  + +L+  Y KEL   A D ++ +IE LL +++ L+      + + +H ++T +E L +   + GS+++ F +++ CAD RPE S++ LI++R +SIH                        A + K L++  F  L+ R + +E+ +N                 +R     +++D+ +  H      L+D  II  + +  + P   +  R +   +      +T   L LL IL   +       A R  + L+                        D L+++  D  + LGL   DG   FSPY +C   E E  +E       +  S P SP         +   V L          ++++CL++E+DW VL LVL R+P  L+ + LI     S+++  + L S           L   P  FS++D H  + PVL AL SY+ +L    Q  ++  LE GL+ + C   C+VAL+  ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV FI   L SN+ + F++  + KDS  R RSTSL+     R   +L +    +AD     Q        H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L
Sbjct:    5 LNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNE---DLHERLEVFKALTDNGRHITYLEEELADFVLQWM-----DVGLSSEFLLVLVNLVKFNSCYLD-EYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSP---ELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLD--IIEKVMARSLSPPPELEERDVAAYSASLEDVKT-AVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSE-------KKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLE-RLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHR-CASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPE-KDSF-RARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKL 974          

HSP 2 Score: 243.432 bits (620), Expect = 1.591e-63
Identity = 130/313 (41.53%), Postives = 189/313 (60.38%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKIT-ENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELK----EPLGHVMS---LSILVRQWALHCNLAAQIQKSLSNKMP-YSSNWLZXLRAIKRLKEKLQKE 1534
            R +TIS S+P  R            V R  +    +    +KV GI+P FVFL LY S  FG+ ++  KP+LLP+   + E S++ LD+IP+++THKI VLY+G  Q N E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ +FH+ATLMP  D D     KKRH+GN++ +IVYNDS    E +K+GT++G+F    +++TPLD   N V + C+ +++      +  ++S   L  + RQ ALH N+A+Q+  S SN    Y S W+  LR IKRL++++ +E
Sbjct: 1344 RGYTISDSAPSRR---------GKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESN--KPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDS---GEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEE 1642          
BLAST of EMLSAG00000002599 vs. GO
Match: - (symbol:TSC2 "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0005096 "GTPase activator activity" evidence=IEA] [GO:0005634 "nucleus" evidence=IEA] [GO:0032007 "negative regulation of TOR signaling" evidence=IEA] [GO:0033596 "TSC1-TSC2 complex" evidence=IEA] [GO:0051056 "regulation of small GTPase mediated signal transduction" evidence=IEA] InterPro:IPR000331 InterPro:IPR003913 InterPro:IPR016024 InterPro:IPR018515 InterPro:IPR027107 Pfam:PF02145 Pfam:PF03542 PRINTS:PR01431 PROSITE:PS50085 GO:GO:0005634 SUPFAM:SSF48371 Gene3D:1.25.10.10 InterPro:IPR011989 GO:GO:0005096 GO:GO:0051056 GO:GO:0032007 PANTHER:PTHR10063 GeneTree:ENSGT00390000017866 GO:GO:0033596 OrthoDB:EOG7KM5S3 InterPro:IPR024584 PANTHER:PTHR10063:SF1 Pfam:PF11864 EMBL:AAEX03004629 EMBL:AAEX03004628 Ensembl:ENSCAFT00000045663 Uniprot:J9NXS0)

HSP 1 Score: 486.878 bits (1252), Expect = 2.927e-142
Identity = 304/995 (30.55%), Postives = 516/995 (51.86%), Query Frame = 0
Query:   50 LQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRND-SPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIH------------------------AAKSKWLNIFSELV---ERYYKKE----------------KRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAER--DSLLK-----------------------DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACL-----LVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSA-ARRPNVALAAKKDVAADVPKXTQI--HPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTML 967
            +  R++++  + E  +  + ++  VE L     DLL+ +  P+ RH VL+  +++V GQ  RLG++R+ FF  IK+  +     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YLD + + +++ +I  LC  T    + +  L+VL++++CY+ +P   L LFI  LCR +N++   +  W + R L+GTHLGHSA+Y +  I++      D  L+RGA+FF+G +LWG +R+ SL  S  ++LP+F  A+ C + +V +E++  + +L+  Y +EL A   D ++ ++E LL +++ L+      + + +H ++T +E L +   + GS+++ F +++ CAD RPE S++ LIT+R +SIH                        A + K L++ S ++    ++Y++E                K   +R     +++D+ +  H      L+D  I+  + +  + P   +  R + V +      +T   L LL IL   +       A R  ++L+                        D L+++  D  + LGL   DG+  FSPY +C + E E  +E       + AS P SP         +   V L          ++++CL+ E DW VL LVL+++P  L+ + LI     S+++  + L S           L  TP  FS++D H  + PVL AL SY+ +L    Q  ++  LE GL+ + C   C+VAL   ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV +I   L SN+ + F++  + KDS  R RSTSL+   +R   +L +    +AD     Q   +    H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L
Sbjct:   54 INNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANE---DLHERLEVFKALTDNGRHITYLEEELAGFVLQWM-----DIGLSSEFLLVLVNLVKFNSCYLD-EYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSP---ELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLD--IVEKVIARSLSPPPELEERDVAVYSASLEDVKT-AVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSE-------KKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLE-RLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYR-CASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPE-KDSF-RARSTSLNERPKRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKL 1022          

HSP 2 Score: 243.817 bits (621), Expect = 1.278e-63
Identity = 137/342 (40.06%), Postives = 198/342 (57.89%), Query Frame = 0
Query: 1202 KKNQNNIPLNSSPNSSFESNL-NEPAMRTTRSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELK----EPLGHVMS---LSILVRQWALHCNLAAQIQKSLSNKMP-YSSNWLZXLRAIKRLKEKLQKE 1534
            ++NQ   P  + P     S+    P+    R +TIS S+P  R            V R            +KV GI+P FVFL LY S  FG+ ++  KP+LLP N+  E S++ LD+IP+++THKI VLY+G  Q + E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ +FH+ATLMP  + D     KKRH+GN++ +IVYNDS    E +K+GT+RG+F    ++ITPLD   N V + C+ +++      +  ++S   L  + RQ ALH N+A+Q+  S SN    Y S W+  LR IKRL+ ++++E
Sbjct: 1370 EENQGQGP--AQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRR---------GKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESN--KPILLP-NESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDS---GEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREE 1694          
BLAST of EMLSAG00000002599 vs. GO
Match: - (symbol:TSC2 "Uncharacterized protein" species:9031 "Gallus gallus" [GO:0001666 "response to hypoxia" evidence=IEA] [GO:0001843 "neural tube closure" evidence=IEA] [GO:0005096 "GTPase activator activity" evidence=IEA] [GO:0005634 "nucleus" evidence=IEA] [GO:0005794 "Golgi apparatus" evidence=IEA] [GO:0006469 "negative regulation of protein kinase activity" evidence=IEA] [GO:0006606 "protein import into nucleus" evidence=IEA] [GO:0007507 "heart development" evidence=IEA] [GO:0008285 "negative regulation of cell proliferation" evidence=IEA] [GO:0014065 "phosphatidylinositol 3-kinase signaling" evidence=IEA] [GO:0014067 "negative regulation of phosphatidylinositol 3-kinase signaling" evidence=IEA] [GO:0016020 "membrane" evidence=IEA] [GO:0019902 "phosphatase binding" evidence=IEA] [GO:0030100 "regulation of endocytosis" evidence=IEA] [GO:0032007 "negative regulation of TOR signaling" evidence=IEA] [GO:0033596 "TSC1-TSC2 complex" evidence=IEA] [GO:0042803 "protein homodimerization activity" evidence=IEA] [GO:0043491 "protein kinase B signaling" evidence=IEA] [GO:0046626 "regulation of insulin receptor signaling pathway" evidence=IEA] [GO:0048009 "insulin-like growth factor receptor signaling pathway" evidence=IEA] [GO:0048471 "perinuclear region of cytoplasm" evidence=IEA] [GO:0050918 "positive chemotaxis" evidence=IEA] [GO:0051056 "regulation of small GTPase mediated signal transduction" evidence=IEA] [GO:0051898 "negative regulation of protein kinase B signaling" evidence=IEA] [GO:0071889 "14-3-3 protein binding" evidence=IEA] InterPro:IPR000331 InterPro:IPR003913 InterPro:IPR016024 InterPro:IPR018515 InterPro:IPR027107 Pfam:PF02145 Pfam:PF03542 PRINTS:PR01431 PROSITE:PS50085 GO:GO:0005634 GO:GO:0005794 GO:GO:0048471 SUPFAM:SSF48371 GO:GO:0016020 Gene3D:1.25.10.10 InterPro:IPR011989 GO:GO:0005096 GO:GO:0051056 GO:GO:0032007 TreeFam:TF324484 PANTHER:PTHR10063 GeneTree:ENSGT00390000017866 GO:GO:0033596 OMA:VDKHRCD OrthoDB:EOG7KM5S3 InterPro:IPR024584 PANTHER:PTHR10063:SF1 Pfam:PF11864 EMBL:AADN03006815 Ensembl:ENSGALT00000009056 PRO:PR:F1NGI4 ArrayExpress:F1NGI4 Uniprot:F1NGI4)

HSP 1 Score: 481.1 bits (1237), Expect = 6.505e-140
Identity = 322/1103 (29.19%), Postives = 555/1103 (50.32%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWF-----KGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLIT------------------------FRQKSIHAAKSKWLNIFSEL--VERYYKKEKRVNIRIKT------------------------------------LDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKR------SLEQEAERDSLLKDLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVS---LNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPL-VSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSAARRPN---VALAAKKDVAADVPKXTQIHPLA----------------------------------------------FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCT 976
            M+K    +    EK K        +G+  +      E  ++ E++  +S     L  R++ +  + E  +  ++++  VE +     D+L+ + P + RH VL   +S++ GQ  RLG++R+ FF +IK+  S     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YL+ D V  ++  I  LC  T+   + +  L+VL++++CY+ +P   L +FI  LCR +N++   +  W + R L+GTHLGHSA+Y +  I++     +D AL+RGA+FF+G +LWG +R+ SL  S  ++LP+F+ A+ C + +V +E++  + +L+  Y KEL A   D +++++E LL +++ LE +    + S +H ++T +E L +   + GS+++ F +++ CAD RPE SV+ LI                         FR +S  A + K L++ S +  + R + +E+ +N+ + +                                    LD+I     ++  S   EL +R ++ +  S+  + ++ +    I + T L SL  ++  + +  +L   ++R      SL   +     + D L+++  D  + LGL   DG+  FSPY +C   E E+ A    + ++ + +        S   Q S + +     +    ++++CL++ETDW VL LVL ++P  L+ + L      +I+   + L    TDK +   LH   TP  FS++D H  + PVL AL SY+ +L  A Q  ++  LE GL+ + C   C+VAL+  ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV +I   L SN+ + F++  + KDS  R RSTSL+   RP    +A  AK+ +    P      P A                                               H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L+T++TS  T
Sbjct:    1 MAKPQSKDSGLKEKFKNLLGLGTSRGSSKSSEGKQTEFIITAEILKELSIECG-LSNRIRAISQICEVAKTKKIEEHAVEAIWKVVADMLQPERPAEARHAVLHLLKSIIQGQGERLGILRAHFFKVIKDYPSNE---DLHERLEVFKALTENGRYITYLEEELADFVLQWM-----DVGLSSEFLLVLVNLVKFNSCYLE-DYVADMVHKICILCIQTSSSVDIEISLQVLDAVVCYNCLPSENLPVFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMADAALLRGAVFFVGMALWGAHRLNSLKNSPTSVLPSFLKAMTCPNAVVSYEIVLSITRLIKKYGKELQAVTWDILLDIMERLLQQLQSLESQ---ELKSIVHDLLTTVEELCDQNEFHGSEERFFELVERCADQRPESSVLNLIAYRAQSIHPAKDGWIHNLQMLMERFFRNESRSAVRIKVLDVLSFVLSINRQFYEEELINLVVISQLAHIPEDKDHQVRKLATQLLVDLAEGCNTHHFNSLLDIIEKKVAAHSLSSPSELEERDLLSYSASLE-DVKTAVLGLLIILQTKLYSLPSSHA-MRVYEMLIHHIQRHYMYAYSLAVASSIRLQVFDFLLMLRADSLHRLGLSNKDGAVRFSPYCLCDFVEAEKRA----SEKKPTGTLSPPSGSPSVPSQNSTIRIGHLPYSMVFGVLLQCLKQETDWKVLKLVLNKLPESLRYKVLFLTSPCNIDLLASALSYMLTDKKTTDRLH--GTPEGFSRTDLHLAVVPVLTALISYHNYLDKAKQREIVYCLEHGLIYR-CANQCVVALSVCSVEMPDIIIKALPVLIVKLTHISATANMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPE-KDSF-RARSTSLN--ERPKSLRLAKNAKQGLNNSPPVKELKEPSAVDAFRSRSISVSEHVVRSRIQTSITSSSLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTITTSVGT 1077          

HSP 2 Score: 248.44 bits (633), Expect = 6.001e-65
Identity = 140/338 (41.42%), Postives = 198/338 (58.58%), Query Frame = 0
Query: 1211 NSSPNSSFESNLNEPAMRTTRSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPE------LKEPLGHVMS---LSILVRQWALHCNLAAQIQKSLSNKM-PYSSNWLZXLRAIKRLKEKLQKENEMK 1538
             S P +S  S    P+    R +TIS S+P  R   K ++       R       +    +KV GI+P FVFL LY S  FG+  +  KPLLLP N+  E S++ LD+IP+++THKI VLY+G  Q N+E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ IFH+ATLMP  D D     KKRH+GN++ +IVYNDS    E +K+GT++G+F    ++ITPLD   N V + C+ +      +   +  ++S   L  + RQ ALH N+A+Q+  S SN    Y S W+  LR IKRL+ +L++E + +
Sbjct: 1470 GSGPTTSPRS----PSGHRPRGYTISDSAPSRR--GKRME-------RDAFKSRTAATNAEKVPGINPSFVFLQLYHSPFFGDENN--KPLLLP-NETFEKSVQLLDQIPSYDTHKIAVLYVGEGQSNNEIAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKIYLGGLDVCGEDGQFTYCWHDDIMQAIFHIATLMPTKDLDKYRCDKKRHLGNDFVSIVYNDS---GEDFKLGTIKGQFNFVHVIITPLDYDCNLVTLQCRKDEDMEGLVDTSVAKIISDKNLPFVARQMALHANMASQVHHSRSNPTDTYPSKWIARLRHIKRLRHRLREETQYQ 1788          
BLAST of EMLSAG00000002599 vs. GO
Match: - (symbol:TSC2 "Uncharacterized protein" species:9913 "Bos taurus" [GO:0001666 "response to hypoxia" evidence=IEA] [GO:0001843 "neural tube closure" evidence=IEA] [GO:0005096 "GTPase activator activity" evidence=IEA] [GO:0005634 "nucleus" evidence=IEA] [GO:0005794 "Golgi apparatus" evidence=IEA] [GO:0006469 "negative regulation of protein kinase activity" evidence=IEA] [GO:0006606 "protein import into nucleus" evidence=IEA] [GO:0007507 "heart development" evidence=IEA] [GO:0008285 "negative regulation of cell proliferation" evidence=IEA] [GO:0014065 "phosphatidylinositol 3-kinase signaling" evidence=IEA] [GO:0014067 "negative regulation of phosphatidylinositol 3-kinase signaling" evidence=IEA] [GO:0016020 "membrane" evidence=IEA] [GO:0019902 "phosphatase binding" evidence=IEA] [GO:0030100 "regulation of endocytosis" evidence=IEA] [GO:0032007 "negative regulation of TOR signaling" evidence=IEA] [GO:0033596 "TSC1-TSC2 complex" evidence=IEA] [GO:0042803 "protein homodimerization activity" evidence=IEA] [GO:0043491 "protein kinase B signaling" evidence=IEA] [GO:0046626 "regulation of insulin receptor signaling pathway" evidence=IEA] [GO:0048009 "insulin-like growth factor receptor signaling pathway" evidence=IEA] [GO:0048471 "perinuclear region of cytoplasm" evidence=IEA] [GO:0050918 "positive chemotaxis" evidence=IEA] [GO:0051056 "regulation of small GTPase mediated signal transduction" evidence=IEA] [GO:0051898 "negative regulation of protein kinase B signaling" evidence=IEA] [GO:0071889 "14-3-3 protein binding" evidence=IEA] InterPro:IPR000331 InterPro:IPR003913 InterPro:IPR016024 InterPro:IPR018515 InterPro:IPR027107 Pfam:PF02145 Pfam:PF03542 PRINTS:PR01431 PROSITE:PS50085 GO:GO:0005634 GO:GO:0005794 GO:GO:0048471 GO:GO:0008285 GO:GO:0007507 SUPFAM:SSF48371 GO:GO:0016020 Gene3D:1.25.10.10 InterPro:IPR011989 GO:GO:0005096 GO:GO:0006469 GO:GO:0014065 GO:GO:0001666 GO:GO:0051056 GO:GO:0032007 GO:GO:0030100 GO:GO:0006606 GO:GO:0001843 GO:GO:0048009 GO:GO:0043491 GO:GO:0050918 GO:GO:0051898 GO:GO:0014067 GO:GO:0046626 TreeFam:TF324484 PANTHER:PTHR10063 GeneTree:ENSGT00390000017866 GO:GO:0033596 OMA:VDKHRCD OrthoDB:EOG7KM5S3 InterPro:IPR024584 PANTHER:PTHR10063:SF1 Pfam:PF11864 EMBL:DAAA02057309 Ensembl:ENSBTAT00000049485 Uniprot:E1BNT2)

HSP 1 Score: 480.33 bits (1235), Expect = 8.759e-140
Identity = 312/1039 (30.03%), Postives = 518/1039 (49.86%), Query Frame = 0
Query:   50 LQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRND-SPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIH------------------------AAKSKWLNI--FSELVERYYKKEKRVN-----------------IRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAER--DSLLK-----------------------DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACL-----LVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSA-------ARRPNVAL-------AAKKDVAADVPKXTQI-------------------------HPLA--------FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTML 967
            L  R++++  + E  R  + ++  VE L     DLL+ +  P+ RH VL+  +++V GQ  RLG++R+ FF +IK+  S     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YLD + + +++ ++  LC  T    + +  L+VL++++CY+ +P   L LFI  LCR +N++   +  W + R L+GTHLGHSA+Y +  I++      D  L+RGA+FF+G +LWG +R+ SL  S  ++LP+F  A+ C + +V +E++  + +L+  Y +EL A   D ++ +IE LL +++ L+      +S+ +H +++ +E L +   + GS+++ F +++ CAD RPE S++ LIT+R +SIH                        A + K L++  F  L+ R + +E+ +N                 +R     +++D+ +  H      L+D  I+  + +  + P   +  R +   +      +T   L LL IL   +       A R  ++L+                        D L+ +  D  + LGL   DG   FSPY +C   E E  +E       + A  P SP         +   V L          ++++CL++ETDW VL LVL+++P  L+ + LI     S+++    L S           L  TP  FS++D H  + PVL AL SY+ +L    Q  ++  LE GL+ + C   C+VAL   ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV +I   L SN+ + F++  + KDS  R RSTSL+        AR P   L         K+  AAD  +   I                         + +A         H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L
Sbjct:   54 LNNRIRVIGQICEVARTKKFEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNE---DLHERLEVFKALTDNGRHITYLEEELADFVLQWM-----DVGLSSEFLLVLVNLVKFNSCYLD-EYIASMVHMVCLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYHMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIIERLLQQLQSLDSP---ELSAIVHDLLSTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIHNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDRDHQVRKLATQLLVDLAEGCHTHHFNSLLD--IVEKVIARSLSPPPELEERDVAAYSASLEDVKT-AVLGLLVILQTKLYALPASHATRVYETLVSHIQLHYRHSYTLPIASSIRLQAFDFLLQLRADSLHRLGLPSKDGLVRFSPYCVCDCLETERSSE-------KKAGCPLSPPAGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSAALCSMLSGPKTLE-RLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYR-CASQCVVALAVCSVEMPDVILKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPE-KDSF-RARSTSLNERPKSLRIARPPKQGLNNSPPVKELKESSAADAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKL 1066          

HSP 2 Score: 247.284 bits (630), Expect = 1.178e-64
Identity = 141/343 (41.11%), Postives = 197/343 (57.43%), Query Frame = 0
Query: 1201 RKKNQNNIPLNSS-PNSSFESNLNEPAMRTTRSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHVMS-------LSILVRQWALHCNLAAQIQKSLSNKMP-YSSNWLZXLRAIKRLKEKLQKE 1534
            R   Q   PL SS P S        P+    R +TIS S+P  R            V R            +KV GI+P FVFL LY S  FG+ ++  KP+LLP N+  E S++ LD+IP+++THKI VLY+G  Q N E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ +FH+ATLMP  D D     KKRH+GN++ +IVYNDS    E +K+GT++G+F    ++ITPLD   N V + C+ +++  +   M+       L  + RQ ALH N+A+Q+  S SN    Y S W+  LR IKRL++++++E
Sbjct: 1443 RGPAQPEGPLPSSCPRS--------PSGLRPRGYTISDSAPSRR---------GKRVERDAFKNRAGTSNTEKVPGINPSFVFLQLYHSPFFGDESN--KPILLP-NESFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDS---GEDFKLGTIKGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSMAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREE 1762          
BLAST of EMLSAG00000002599 vs. GO
Match: - (symbol:Tsc2 "tuberous sclerosis 2" species:10090 "Mus musculus" [GO:0001666 "response to hypoxia" evidence=IMP] [GO:0001843 "neural tube closure" evidence=IMP] [GO:0005096 "GTPase activator activity" evidence=ISO;IBA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005622 "intracellular" evidence=IGI] [GO:0005623 "cell" evidence=IMP] [GO:0005634 "nucleus" evidence=ISO;IBA] [GO:0005737 "cytoplasm" evidence=ISO;IDA] [GO:0005829 "cytosol" evidence=ISO] [GO:0005856 "cytoskeleton" evidence=ISO] [GO:0005901 "caveola" evidence=ISO] [GO:0006469 "negative regulation of protein kinase activity" evidence=IMP] [GO:0006606 "protein import into nucleus" evidence=IMP] [GO:0006953 "acute-phase response" evidence=ISO] [GO:0007165 "signal transduction" evidence=ISO] [GO:0007507 "heart development" evidence=IMP] [GO:0008104 "protein localization" evidence=IDA] [GO:0008285 "negative regulation of cell proliferation" evidence=ISO;IGI;IMP] [GO:0014065 "phosphatidylinositol 3-kinase signaling" evidence=IMP] [GO:0014067 "negative regulation of phosphatidylinositol 3-kinase signaling" evidence=IMP] [GO:0016020 "membrane" evidence=ISO] [GO:0019902 "phosphatase binding" evidence=ISO] [GO:0030030 "cell projection organization" evidence=ISO] [GO:0030100 "regulation of endocytosis" evidence=IMP] [GO:0030178 "negative regulation of Wnt signaling pathway" evidence=ISO;IBA] [GO:0030425 "dendrite" evidence=ISO] [GO:0030426 "growth cone" evidence=ISO] [GO:0032007 "negative regulation of TOR signaling" evidence=ISO;IMP] [GO:0033596 "TSC1-TSC2 complex" evidence=ISO;IBA] [GO:0042803 "protein homodimerization activity" evidence=ISO] [GO:0043005 "neuron projection" evidence=ISO] [GO:0043025 "neuronal cell body" evidence=ISO] [GO:0043231 "intracellular membrane-bounded organelle" evidence=ISO] [GO:0043234 "protein complex" evidence=ISO] [GO:0043407 "negative regulation of MAP kinase activity" evidence=ISO] [GO:0043491 "protein kinase B signaling" evidence=IGI;IMP] [GO:0043547 "positive regulation of GTPase activity" evidence=ISO;IBA] [GO:0045121 "membrane raft" evidence=ISO] [GO:0045184 "establishment of protein localization" evidence=ISO] [GO:0045792 "negative regulation of cell size" evidence=ISO;IBA] [GO:0046626 "regulation of insulin receptor signaling pathway" evidence=IMP] [GO:0046982 "protein heterodimerization activity" evidence=ISO] [GO:0048009 "insulin-like growth factor receptor signaling pathway" evidence=IGI;IMP] [GO:0048471 "perinuclear region of cytoplasm" evidence=ISO] [GO:0050680 "negative regulation of epithelial cell proliferation" evidence=ISO] [GO:0050918 "positive chemotaxis" evidence=IMP] [GO:0051056 "regulation of small GTPase mediated signal transduction" evidence=IEA] [GO:0051260 "protein homooligomerization" evidence=ISO] [GO:0051291 "protein heterooligomerization" evidence=ISO] [GO:0051726 "regulation of cell cycle" evidence=ISO;IBA] [GO:0051898 "negative regulation of protein kinase B signaling" evidence=IGI;IMP] [GO:0071889 "14-3-3 protein binding" evidence=IDA] InterPro:IPR000331 InterPro:IPR003913 InterPro:IPR016024 InterPro:IPR018515 InterPro:IPR027107 Pfam:PF02145 Pfam:PF03542 PRINTS:PR01431 PROSITE:PS50085 MGI:MGI:102548 GO:GO:0005634 GO:GO:0008285 GO:GO:0007507 SUPFAM:SSF48371 GO:GO:0016020 Gene3D:1.25.10.10 InterPro:IPR011989 GO:GO:0005096 GO:GO:0006469 GO:GO:0014065 GO:GO:0001666 GO:GO:0051056 GO:GO:0032007 GO:GO:0051726 GO:GO:0030100 GO:GO:0006606 GO:GO:0001843 GO:GO:0030030 GO:GO:0048009 GO:GO:0045792 GO:GO:0043491 GO:GO:0050918 GO:GO:0030178 GO:GO:0071889 GO:GO:0019902 GO:GO:0051898 GO:GO:0014067 GO:GO:0046626 ChiTaRS:TSC2 EMBL:AB009371 UniGene:Mm.30435 PANTHER:PTHR10063 GO:GO:0033596 eggNOG:NOG290929 HOVERGEN:HBG018005 InterPro:IPR024584 PANTHER:PTHR10063:SF1 Pfam:PF11864 EMBL:U37775 EMBL:U39818 EMBL:AF132986 ProteinModelPortal:Q61037 SMR:Q61037 IntAct:Q61037 MINT:MINT-5068541 PhosphoSite:Q61037 PaxDb:Q61037 PRIDE:Q61037 InParanoid:Q61037 PRO:PR:Q61037 CleanEx:MM_TSC2 Genevestigator:Q61037 Uniprot:Q61037)

HSP 1 Score: 476.863 bits (1226), Expect = 1.518e-138
Identity = 324/1088 (29.78%), Postives = 541/1088 (49.72%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIR-----LSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRND-SPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKW------------------------LNI--FSELVERYYKKEKRVN-----------------IRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAER--DSLLK-----------------------DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSA-------ARRPNVAL-------AAKKDVAADVPKXTQI-------------------------HPLA--------FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTML 967
            M+K +  +    EK K       S   P   E +     ++ E++  +S     L  R++++  + +  +  +L++  VE L     DLL+ +  P+ RH VL+  +++V GQ  RLG++R+ FF +IK+  S     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YLD + + +++ +I  LC  T    + +  L+VL++++CY+ +P   L LFI  LCR +N++   +  W + R L+GTHLGHSA+Y +  I++      D  L+RGA+FF+G +LWG +R+ SL  S  ++LP+F  A+ C + +V +E++  + +L+  Y KEL A   D ++++IE LL +++ L+      + + +H ++T +E L +   + GS+++ + +++  AD RPE S++ LI++R +SIH AK  W                        L++  F  L+ R + +E+ +N                 +R     +++D+ +  H      L+D  II  + +  + P   +  R + V +      +T   L LL IL   +       A R  +SL+                        D L+++  D  + LGL   DG   FSPY +C   E +  +E   +      + P SP  +    +   L  SL    LL  +CL++E+DW VL LVL+R+P  L+ + LI     S+++  + L S           L  TP  FS++D H  + PVL AL SY+ +L    Q  ++  LE GL+ + C   C+VAL   ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV +I   L SN+ + F++  + KDS  R RSTSL+        AR P   L         K+  AA+  +   I                         + +A         H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L
Sbjct:    1 MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECG-LNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNE---DLHERLEVFKALTDNGRHITYLEEELAEFVLQWM-----DVGLSSEFLLVLVNLVKFNSCYLD-EYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSP---ELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVAIKVLDVLSFVLLIIRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLD--IIEKVMARSLSPPPELEERDLAVHSASLEDVKT-AVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLL--QCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLE-RLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYR-CASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFVPEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPE-KDSF-RARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKL 1066          

HSP 2 Score: 224.557 bits (571), Expect = 1.145e-57
Identity = 127/321 (39.56%), Postives = 186/321 (57.94%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVR-GKFISACIVITPLDQGSNRVCVDCKPE--------LKEP-------LGHVMSLSILVRQWALHCNLAAQIQKSLSNKMP-YSSNWLZXLRAIKRLKEKLQKE 1534
            R +TIS S+P  R            V R       +    +KV GI+P FVFL LY S  FG+ ++  KP+LLP N+  E S++ LD+IP+++THKI VLY+G  Q + E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ +FH+ATLMP  D D     KKRH+GN++ +I+YNDS    E +K+GT++ G+F    ++ITPLD   N + + C+ +         ++P       L  VM L++ +     H  +A+Q+  S SN    Y S W+  LR IKRL++++++E
Sbjct: 1462 RGYTISDSAPSRR---------GKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESN--KPILLP-NESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDS---GEDFKLGTIKQGQFNFVHVIITPLDYKCNLLTLQCRKDGPACKCEWWRQPGEIVVWALPVVMELTVTI--LLCHLQMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREE 1765          
BLAST of EMLSAG00000002599 vs. GO
Match: - (symbol:Tsc2 "Tuberin" species:10116 "Rattus norvegicus" [GO:0001666 "response to hypoxia" evidence=IEA] [GO:0001843 "neural tube closure" evidence=IEA] [GO:0005634 "nucleus" evidence=IBA] [GO:0005794 "Golgi apparatus" evidence=IEA] [GO:0006606 "protein import into nucleus" evidence=IEA] [GO:0007507 "heart development" evidence=IEA] [GO:0014065 "phosphatidylinositol 3-kinase signaling" evidence=IEA] [GO:0014067 "negative regulation of phosphatidylinositol 3-kinase signaling" evidence=IEA] [GO:0019902 "phosphatase binding" evidence=ISS] [GO:0030030 "cell projection organization" evidence=IGI;IMP] [GO:0030100 "regulation of endocytosis" evidence=IEA] [GO:0032851 "positive regulation of Rab GTPase activity" evidence=TAS] [GO:0033596 "TSC1-TSC2 complex" evidence=IBA] [GO:0042803 "protein homodimerization activity" evidence=IEA] [GO:0043491 "protein kinase B signaling" evidence=IBA] [GO:0045792 "negative regulation of cell size" evidence=IMP] [GO:0046626 "regulation of insulin receptor signaling pathway" evidence=IBA] [GO:0048009 "insulin-like growth factor receptor signaling pathway" evidence=IBA] [GO:0050918 "positive chemotaxis" evidence=IEA] [GO:0051898 "negative regulation of protein kinase B signaling" evidence=IEA] [GO:0071889 "14-3-3 protein binding" evidence=IEA] InterPro:IPR000331 InterPro:IPR003913 InterPro:IPR016024 InterPro:IPR018515 InterPro:IPR027107 Pfam:PF02145 Pfam:PF03542 PRINTS:PR01431 PROSITE:PS50085 RGD:3908 EMBL:D50413 GO:GO:0005634 GO:GO:0005794 GO:GO:0048471 GO:GO:0051291 GO:GO:0007507 SUPFAM:SSF48371 Gene3D:1.25.10.10 InterPro:IPR011989 GO:GO:0005856 GO:GO:0005901 GO:GO:0014065 GO:GO:0043025 GO:GO:0030425 GO:GO:0051260 GO:GO:0006953 GO:GO:0001666 GO:GO:0050680 GO:GO:0032007 GO:GO:0030426 GO:GO:0045184 GO:GO:0051726 GO:GO:0030100 GO:GO:0006606 GO:GO:0043407 GO:GO:0001843 GO:GO:0030030 GO:GO:0048009 GO:GO:0045792 GO:GO:0043491 GO:GO:0050918 GO:GO:0030178 GO:GO:0005097 GO:GO:0019902 GO:GO:0051898 GO:GO:0014067 GO:GO:0046626 TreeFam:TF324484 PANTHER:PTHR10063 GeneTree:ENSGT00390000017866 GO:GO:0033596 CTD:7249 eggNOG:NOG290929 HOVERGEN:HBG018005 KO:K07207 OrthoDB:EOG7KM5S3 InterPro:IPR024584 PANTHER:PTHR10063:SF1 Pfam:PF11864 EMBL:U24150 PIR:S57329 RefSeq:NP_036812.2 UniGene:Rn.5875 ProteinModelPortal:P49816 BioGrid:246972 IntAct:P49816 MINT:MINT-4783802 STRING:10116.ENSRNOP00000016221 PhosphoSite:P49816 PaxDb:P49816 PRIDE:P49816 Ensembl:ENSRNOT00000016221 GeneID:24855 KEGG:rno:24855 UCSC:RGD:3908 HOGENOM:HOG000045987 InParanoid:P49816 NextBio:604654 PRO:PR:P49816 ArrayExpress:P49816 Genevestigator:P49816 Uniprot:P49816)

HSP 1 Score: 476.478 bits (1225), Expect = 2.068e-138
Identity = 323/1093 (29.55%), Postives = 540/1093 (49.41%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIR-----LSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRND-SPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKW------------------------LNI--FSELVERYYKKEKRVN-----------------IRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAER--DSLLK-----------------------DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPE-----EEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSA-------ARRPNVAL-------AAKKDVAADVPKXTQI-------------------------HPLA--------FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTML 967
            M+K +  +    EK K       S   P   E +     ++ E++  +S     L  R++++  + +  +  +L++  VE L     DLL+ +  P+ RH VL+  +++V GQ  RLG++R+ FF +IK+  S     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YLD + +  ++ +I  LC  T    + +  L+VL++++CY+ +P   L LFI  LCR VN++   +  W + R L+GTHLGHSA+Y +  I++      D  L+RGA+FF+G +LWG +R+ SL  S  ++LP+F  A+ C + +V +E++  + +L+  Y KEL A   D ++++IE LL +++ L+      + + +H ++T +E L +   + GS+++ + +++  AD RPE S++ LIT+R +SIH AK  W                        L++  F  L+ R + +E+ +N                 +R     +++D+ +  H      L+D  II  + +  + P   +  R + V +      +T   L LL IL   +       A R  ++L+                        D L+++  D  + LGL   DG   FSPY +C   E     E++ +  L       +  P+ P+   G    S+L   L       ++CL++ETDW VL LVL+++P  L+ + LI     S+++  + L S           L  TP  FS++D H  + PVL AL SY+ +L    Q  ++  LE GL+ + C   C+VAL   ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV +I   L SN+ + F++  + KD   R RSTSL+        AR P   L         K+  AA+  +   I                         + +A         H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L
Sbjct:    1 MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECG-LNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNE---DLHERLEVFKALTDNGRHITYLEEELAEFVLQWM-----DVGLSSEFLLVLVNLVKFNSCYLD-EYIAPMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTVNVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSP---ELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLD--IIEKVMARSLSPPLELEERDLAVYSASLEDVKT-AVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCAELDRASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVL-------LQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLE-RLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYR-CASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPE-KDKF-RARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKL 1066          

HSP 2 Score: 238.039 bits (606), Expect = 9.116e-62
Identity = 128/312 (41.03%), Postives = 188/312 (60.26%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELK----EPLGHVMS---LSILVRQWALHCNLAAQIQKSLSNKMP-YSSNWLZXLRAIKRLKEKLQKE 1534
            R +TIS S+P  R            V R       +    +KV GI+P FVFL LY S   G+ ++  KP+LLP N+  E S++ LD+IP+++THKI VLY+G  Q + E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ +FH+ATLMP  D D     KKRH+GN++ +I+YNDS    E +K+GT++G+F    ++ITPLD   N + + C+ +++      +  ++S   LS + RQ ALH N+A+Q+    SN    Y S W+  LR IKRL++++++E
Sbjct: 1463 RGYTISDSAPSRR---------GKRVERDNFKSRTAASSAEKVPGINPSFVFLQLYHSPFCGDESN--KPILLP-NESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDS---GEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHRRSNPTDIYPSKWIARLRHIKRLRQRIREE 1759          
BLAST of EMLSAG00000002599 vs. GO
Match: - (symbol:Tsc2 "tuberous sclerosis 2" species:10116 "Rattus norvegicus" [GO:0001666 "response to hypoxia" evidence=IEA;ISO] [GO:0001843 "neural tube closure" evidence=IEA;ISO] [GO:0005096 "GTPase activator activity" evidence=IEA;ISO;IDA] [GO:0005097 "Rab GTPase activator activity" evidence=TAS] [GO:0005515 "protein binding" evidence=IPI] [GO:0005622 "intracellular" evidence=ISO] [GO:0005623 "cell" evidence=ISO] [GO:0005634 "nucleus" evidence=IEA;ISO;IBA] [GO:0005737 "cytoplasm" evidence=ISO] [GO:0005794 "Golgi apparatus" evidence=IEA;ISO] [GO:0005829 "cytosol" evidence=ISO;IDA] [GO:0005856 "cytoskeleton" evidence=IDA] [GO:0005901 "caveola" evidence=IDA] [GO:0006469 "negative regulation of protein kinase activity" evidence=ISO] [GO:0006606 "protein import into nucleus" evidence=IEA;ISO] [GO:0006953 "acute-phase response" evidence=IMP] [GO:0007165 "signal transduction" evidence=IDA] [GO:0007507 "heart development" evidence=IEA;ISO] [GO:0008104 "protein localization" evidence=ISO] [GO:0008285 "negative regulation of cell proliferation" evidence=ISO;IMP] [GO:0014065 "phosphatidylinositol 3-kinase signaling" evidence=IEA;ISO] [GO:0014067 "negative regulation of phosphatidylinositol 3-kinase signaling" evidence=IEA;ISO] [GO:0016020 "membrane" evidence=ISO] [GO:0019902 "phosphatase binding" evidence=ISO;ISS] [GO:0030030 "cell projection organization" evidence=IGI;IMP] [GO:0030100 "regulation of endocytosis" evidence=IEA;ISO] [GO:0030178 "negative regulation of Wnt signaling pathway" evidence=IDA] [GO:0030425 "dendrite" evidence=IDA] [GO:0030426 "growth cone" evidence=IDA] [GO:0032007 "negative regulation of TOR signaling" evidence=IEA;ISO;IDA] [GO:0032851 "positive regulation of Rab GTPase activity" evidence=TAS] [GO:0033596 "TSC1-TSC2 complex" evidence=IEA;ISO;IBA] [GO:0042803 "protein homodimerization activity" evidence=IEA;ISO] [GO:0043005 "neuron projection" evidence=IDA] [GO:0043025 "neuronal cell body" evidence=IDA] [GO:0043231 "intracellular membrane-bounded organelle" evidence=IDA] [GO:0043234 "protein complex" evidence=IDA] [GO:0043407 "negative regulation of MAP kinase activity" evidence=IMP] [GO:0043491 "protein kinase B signaling" evidence=ISO;IBA] [GO:0045121 "membrane raft" evidence=IDA] [GO:0045184 "establishment of protein localization" evidence=IMP] [GO:0045792 "negative regulation of cell size" evidence=IMP] [GO:0046626 "regulation of insulin receptor signaling pathway" evidence=ISO;IBA] [GO:0046982 "protein heterodimerization activity" evidence=IPI] [GO:0048009 "insulin-like growth factor receptor signaling pathway" evidence=ISO;IBA] [GO:0048471 "perinuclear region of cytoplasm" evidence=ISO;IDA] [GO:0050680 "negative regulation of epithelial cell proliferation" evidence=IDA] [GO:0050918 "positive chemotaxis" evidence=IEA;ISO] [GO:0051056 "regulation of small GTPase mediated signal transduction" evidence=IEA] [GO:0051260 "protein homooligomerization" evidence=IMP;IDA] [GO:0051291 "protein heterooligomerization" evidence=IMP;IDA] [GO:0051726 "regulation of cell cycle" evidence=IMP] [GO:0051898 "negative regulation of protein kinase B signaling" evidence=IEA;ISO] [GO:0071889 "14-3-3 protein binding" evidence=IEA;ISO] InterPro:IPR000331 InterPro:IPR003913 InterPro:IPR016024 InterPro:IPR018515 InterPro:IPR027107 Pfam:PF02145 Pfam:PF03542 PRINTS:PR01431 PROSITE:PS50085 RGD:3908 EMBL:D50413 GO:GO:0005634 GO:GO:0005794 GO:GO:0048471 GO:GO:0051291 GO:GO:0007507 SUPFAM:SSF48371 Gene3D:1.25.10.10 InterPro:IPR011989 GO:GO:0005856 GO:GO:0005901 GO:GO:0014065 GO:GO:0043025 GO:GO:0030425 GO:GO:0051260 GO:GO:0006953 GO:GO:0001666 GO:GO:0050680 GO:GO:0032007 GO:GO:0030426 GO:GO:0045184 GO:GO:0051726 GO:GO:0030100 GO:GO:0006606 GO:GO:0043407 GO:GO:0001843 GO:GO:0030030 GO:GO:0048009 GO:GO:0045792 GO:GO:0043491 GO:GO:0050918 GO:GO:0030178 GO:GO:0005097 GO:GO:0019902 GO:GO:0051898 GO:GO:0014067 GO:GO:0046626 TreeFam:TF324484 PANTHER:PTHR10063 GeneTree:ENSGT00390000017866 GO:GO:0033596 CTD:7249 eggNOG:NOG290929 HOVERGEN:HBG018005 KO:K07207 OrthoDB:EOG7KM5S3 InterPro:IPR024584 PANTHER:PTHR10063:SF1 Pfam:PF11864 EMBL:U24150 PIR:S57329 RefSeq:NP_036812.2 UniGene:Rn.5875 ProteinModelPortal:P49816 BioGrid:246972 IntAct:P49816 MINT:MINT-4783802 STRING:10116.ENSRNOP00000016221 PhosphoSite:P49816 PaxDb:P49816 PRIDE:P49816 Ensembl:ENSRNOT00000016221 GeneID:24855 KEGG:rno:24855 UCSC:RGD:3908 HOGENOM:HOG000045987 InParanoid:P49816 NextBio:604654 PRO:PR:P49816 ArrayExpress:P49816 Genevestigator:P49816 Uniprot:P49816)

HSP 1 Score: 476.478 bits (1225), Expect = 2.068e-138
Identity = 323/1093 (29.55%), Postives = 540/1093 (49.41%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIR-----LSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRND-SPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKW------------------------LNI--FSELVERYYKKEKRVN-----------------IRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAER--DSLLK-----------------------DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPE-----EEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSA-------ARRPNVAL-------AAKKDVAADVPKXTQI-------------------------HPLA--------FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTML 967
            M+K +  +    EK K       S   P   E +     ++ E++  +S     L  R++++  + +  +  +L++  VE L     DLL+ +  P+ RH VL+  +++V GQ  RLG++R+ FF +IK+  S     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YLD + +  ++ +I  LC  T    + +  L+VL++++CY+ +P   L LFI  LCR VN++   +  W + R L+GTHLGHSA+Y +  I++      D  L+RGA+FF+G +LWG +R+ SL  S  ++LP+F  A+ C + +V +E++  + +L+  Y KEL A   D ++++IE LL +++ L+      + + +H ++T +E L +   + GS+++ + +++  AD RPE S++ LIT+R +SIH AK  W                        L++  F  L+ R + +E+ +N                 +R     +++D+ +  H      L+D  II  + +  + P   +  R + V +      +T   L LL IL   +       A R  ++L+                        D L+++  D  + LGL   DG   FSPY +C   E     E++ +  L       +  P+ P+   G    S+L   L       ++CL++ETDW VL LVL+++P  L+ + LI     S+++  + L S           L  TP  FS++D H  + PVL AL SY+ +L    Q  ++  LE GL+ + C   C+VAL   ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV +I   L SN+ + F++  + KD   R RSTSL+        AR P   L         K+  AA+  +   I                         + +A         H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L
Sbjct:    1 MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECG-LNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNE---DLHERLEVFKALTDNGRHITYLEEELAEFVLQWM-----DVGLSSEFLLVLVNLVKFNSCYLD-EYIAPMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTVNVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSP---ELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLD--IIEKVMARSLSPPLELEERDLAVYSASLEDVKT-AVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCAELDRASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVL-------LQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLE-RLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYR-CASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPE-KDKF-RARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKL 1066          

HSP 2 Score: 238.039 bits (606), Expect = 9.116e-62
Identity = 128/312 (41.03%), Postives = 188/312 (60.26%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELK----EPLGHVMS---LSILVRQWALHCNLAAQIQKSLSNKMP-YSSNWLZXLRAIKRLKEKLQKE 1534
            R +TIS S+P  R            V R       +    +KV GI+P FVFL LY S   G+ ++  KP+LLP N+  E S++ LD+IP+++THKI VLY+G  Q + E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ +FH+ATLMP  D D     KKRH+GN++ +I+YNDS    E +K+GT++G+F    ++ITPLD   N + + C+ +++      +  ++S   LS + RQ ALH N+A+Q+    SN    Y S W+  LR IKRL++++++E
Sbjct: 1463 RGYTISDSAPSRR---------GKRVERDNFKSRTAASSAEKVPGINPSFVFLQLYHSPFCGDESN--KPILLP-NESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDS---GEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHRRSNPTDIYPSKWIARLRHIKRLRQRIREE 1759          
BLAST of EMLSAG00000002599 vs. GO
Match: - (symbol:TSC2 "Tuberin" species:9606 "Homo sapiens" [GO:0001666 "response to hypoxia" evidence=IEA] [GO:0001843 "neural tube closure" evidence=ISS] [GO:0005096 "GTPase activator activity" evidence=IDA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005634 "nucleus" evidence=IDA] [GO:0005737 "cytoplasm" evidence=IDA] [GO:0005794 "Golgi apparatus" evidence=IDA] [GO:0005829 "cytosol" evidence=IDA;TAS] [GO:0005901 "caveola" evidence=IEA] [GO:0006469 "negative regulation of protein kinase activity" evidence=ISS] [GO:0006606 "protein import into nucleus" evidence=ISS] [GO:0006897 "endocytosis" evidence=TAS] [GO:0006953 "acute-phase response" evidence=IEA] [GO:0007050 "cell cycle arrest" evidence=TAS] [GO:0007173 "epidermal growth factor receptor signaling pathway" evidence=TAS] [GO:0007507 "heart development" evidence=ISS] [GO:0008104 "protein localization" evidence=ISS] [GO:0008285 "negative regulation of cell proliferation" evidence=ISS;IBA] [GO:0008286 "insulin receptor signaling pathway" evidence=TAS] [GO:0008543 "fibroblast growth factor receptor signaling pathway" evidence=TAS] [GO:0014065 "phosphatidylinositol 3-kinase signaling" evidence=IEA] [GO:0014067 "negative regulation of phosphatidylinositol 3-kinase signaling" evidence=ISS] [GO:0016020 "membrane" evidence=IDA] [GO:0016192 "vesicle-mediated transport" evidence=TAS] [GO:0019902 "phosphatase binding" evidence=IDA] [GO:0030030 "cell projection organization" evidence=IEA] [GO:0030100 "regulation of endocytosis" evidence=ISS] [GO:0030178 "negative regulation of Wnt signaling pathway" evidence=IBA] [GO:0030425 "dendrite" evidence=IEA] [GO:0030426 "growth cone" evidence=IEA] [GO:0032007 "negative regulation of TOR signaling" evidence=IBA] [GO:0033596 "TSC1-TSC2 complex" evidence=IDA] [GO:0038095 "Fc-epsilon receptor signaling pathway" evidence=TAS] [GO:0042803 "protein homodimerization activity" evidence=IPI] [GO:0043025 "neuronal cell body" evidence=IEA] [GO:0043407 "negative regulation of MAP kinase activity" evidence=IEA] [GO:0043491 "protein kinase B signaling" evidence=ISS;IBA] [GO:0045087 "innate immune response" evidence=TAS] [GO:0045792 "negative regulation of cell size" evidence=IBA] [GO:0046626 "regulation of insulin receptor signaling pathway" evidence=ISS;IBA] [GO:0046982 "protein heterodimerization activity" evidence=IEA] [GO:0048009 "insulin-like growth factor receptor signaling pathway" evidence=ISS;IBA] [GO:0048011 "neurotrophin TRK receptor signaling pathway" evidence=TAS] [GO:0048015 "phosphatidylinositol-mediated signaling" evidence=TAS] [GO:0048471 "perinuclear region of cytoplasm" evidence=IDA] [GO:0050680 "negative regulation of epithelial cell proliferation" evidence=IEA] [GO:0050918 "positive chemotaxis" evidence=ISS] [GO:0051056 "regulation of small GTPase mediated signal transduction" evidence=IEA] [GO:0051260 "protein homooligomerization" evidence=IEA] [GO:0051291 "protein heterooligomerization" evidence=IEA] [GO:0051726 "regulation of cell cycle" evidence=IBA] [GO:0051898 "negative regulation of protein kinase B signaling" evidence=ISS] [GO:0071889 "14-3-3 protein binding" evidence=IEA] InterPro:IPR000331 InterPro:IPR003913 InterPro:IPR016024 InterPro:IPR018515 InterPro:IPR027107 Pfam:PF02145 Pfam:PF03542 PRINTS:PR01431 PROSITE:PS50085 GO:GO:0005634 GO:GO:0005794 GO:GO:0048471 Reactome:REACT_111102 Reactome:REACT_116125 Reactome:REACT_6900 GO:GO:0007173 GO:GO:0038095 GO:GO:0008543 GO:GO:0045087 GO:GO:0008286 GO:GO:0048011 GO:GO:0051291 GO:GO:0008285 GO:GO:0007507 SUPFAM:SSF48371 GO:GO:0016020 Gene3D:1.25.10.10 InterPro:IPR011989 GO:GO:0005096 GO:GO:0006469 EMBL:CH471112 GO:GO:0005901 GO:GO:0014065 GO:GO:0043025 GO:GO:0030425 GO:GO:0051260 GO:GO:0006953 GO:GO:0001666 GO:GO:0051056 GO:GO:0050680 GO:GO:0006897 GO:GO:0007050 GO:GO:0032007 GO:GO:0030426 GO:GO:0030100 GO:GO:0006606 GO:GO:0043407 GO:GO:0001843 GO:GO:0048015 GO:GO:0030030 GO:GO:0048009 GO:GO:0045792 GO:GO:0043491 GO:GO:0050918 GO:GO:0030178 GO:GO:0019902 GO:GO:0051898 GO:GO:0014067 GO:GO:0046626 EMBL:AB014460 EMBL:AC005600 EMBL:AC093513 Orphanet:88924 TreeFam:TF324484 PANTHER:PTHR10063 Orphanet:538 Orphanet:805 GO:GO:0033596 EMBL:X75621 EMBL:L48546 EMBL:L48517 EMBL:L48518 EMBL:L48519 EMBL:L48521 EMBL:L48522 EMBL:L48523 EMBL:L48524 EMBL:L48525 EMBL:L48526 EMBL:L48527 EMBL:L48528 EMBL:L48529 EMBL:L48530 EMBL:L48531 EMBL:L48532 EMBL:L48533 EMBL:L48534 EMBL:L48535 EMBL:L48536 EMBL:L48537 EMBL:L48538 EMBL:L48539 EMBL:L48540 EMBL:L48541 EMBL:L48542 EMBL:L48543 EMBL:L48544 EMBL:L48545 EMBL:AB210000 EMBL:AK295728 EMBL:AK299343 EMBL:BC150300 EMBL:BC025364 EMBL:BC046929 PIR:A49420 RefSeq:NP_000539.2 RefSeq:NP_001070651.1 RefSeq:NP_001107854.1 UniGene:Hs.90303 ProteinModelPortal:P49815 SMR:P49815 BioGrid:113100 IntAct:P49815 MINT:MINT-250244 STRING:9606.ENSP00000219476 PhosphoSite:P49815 DMDM:269849475 PaxDb:P49815 PRIDE:P49815 Ensembl:ENST00000219476 Ensembl:ENST00000350773 Ensembl:ENST00000353929 Ensembl:ENST00000401874 Ensembl:ENST00000439673 GeneID:7249 KEGG:hsa:7249 UCSC:uc002con.3 UCSC:uc002coo.3 UCSC:uc002cop.3 UCSC:uc010bsd.3 UCSC:uc010uvv.2 CTD:7249 GeneCards:GC16P002097 HGNC:HGNC:12363 HPA:CAB002225 HPA:HPA030409 HPA:HPA049679 MIM:191092 MIM:606690 MIM:613254 neXtProt:NX_P49815 PharmGKB:PA37035 eggNOG:NOG290929 HOVERGEN:HBG018005 InParanoid:P49815 KO:K07207 OMA:VDKHRCD OrthoDB:EOG7KM5S3 PhylomeDB:P49815 SignaLink:P49815 GeneWiki:TSC2 GenomeRNAi:7249 NextBio:28347 PRO:PR:P49815 ArrayExpress:P49815 Bgee:P49815 CleanEx:HS_TSC2 Genevestigator:P49815 InterPro:IPR024584 PANTHER:PTHR10063:SF1 Pfam:PF11864 Uniprot:P49815)

HSP 1 Score: 475.707 bits (1223), Expect = 3.148e-138
Identity = 311/1039 (29.93%), Postives = 515/1039 (49.57%), Query Frame = 0
Query:   50 LQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIH------------------------AAKSKWLNI--FSELVERYYKKEKRVN-----------------IRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAER--DSLLK-----------------------DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACL-----LVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSA-------ARRPNVAL-------AAKKDVAADVPKXTQI----HPLA-----------------------------FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTML 967
            L  R++++  + E  +  + ++  VE L     DLL+ + P + RH VL+  +++V GQ  RLG++R+ FF +IK+  S     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YLD + +  ++ +I  LC  T    + +  L+VL++++CY+ +P   L LFI  LCR +N++   +  W + R L+GTHLGHSA+Y +  +++      D  L+RGA+FF+G +LWG +R+ SL  S  ++LP+F  A+ C + +V +E++  + +L+  Y KEL   A D ++ +IE LL +++ L+      + + +H ++T +E L +   + GS+++ F +++ CAD RPE S++ LI++R +SIH                        A + K L++  F  L+ R + +E+ +N                 +R     +++D+ +  H      L+D  II  + +  + P   +  R +   +      +T   L LL IL   +       A R  + L+                        D L+++  D  + LGL   DG   FSPY +C   E E  +E       +  S P SP         +   V L          ++++CL++E+DW VL LVL R+P  L+ + LI     S+++  + L S           L   P  FS++D H  + PVL AL SY+ +L    Q  ++  LE GL+ + C   C+VAL+  ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV FI   L SN+ + F++  + KDS  R RSTSL+        AR P   L         K+  AA+  +   I    H +                               H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L
Sbjct:   54 LNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNE---DLHERLEVFKALTDNGRHITYLEEELADFVLQWM-----DVGLSSEFLLVLVNLVKFNSCYLD-EYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSP---ELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLD--IIEKVMARSLSPPPELEERDVAAYSASLEDVKT-AVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSE-------KKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLE-RLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHR-CASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPE-KDSF-RARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKL 1066          

HSP 2 Score: 243.817 bits (621), Expect = 1.588e-63
Identity = 130/313 (41.53%), Postives = 189/313 (60.38%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKIT-ENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELK----EPLGHVMS---LSILVRQWALHCNLAAQIQKSLSNKMP-YSSNWLZXLRAIKRLKEKLQKE 1534
            R +TIS S+P  R            V R  +    +    +KV GI+P FVFL LY S  FG+ ++  KP+LLP+   + E S++ LD+IP+++THKI VLY+G  Q N E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ +FH+ATLMP  D D     KKRH+GN++ +IVYNDS    E +K+GT++G+F    +++TPLD   N V + C+ +++      +  ++S   L  + RQ ALH N+A+Q+  S SN    Y S W+  LR IKRL++++ +E
Sbjct: 1459 RGYTISDSAPSRR---------GKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESN--KPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDS---GEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEE 1757          
BLAST of EMLSAG00000002599 vs. GO
Match: - (symbol:TSC2 "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0001666 "response to hypoxia" evidence=IEA] [GO:0001843 "neural tube closure" evidence=IEA] [GO:0005096 "GTPase activator activity" evidence=IEA] [GO:0005634 "nucleus" evidence=IEA] [GO:0005794 "Golgi apparatus" evidence=IEA] [GO:0006469 "negative regulation of protein kinase activity" evidence=IEA] [GO:0006606 "protein import into nucleus" evidence=IEA] [GO:0007507 "heart development" evidence=IEA] [GO:0008285 "negative regulation of cell proliferation" evidence=IEA] [GO:0014065 "phosphatidylinositol 3-kinase signaling" evidence=IEA] [GO:0014067 "negative regulation of phosphatidylinositol 3-kinase signaling" evidence=IEA] [GO:0016020 "membrane" evidence=IEA] [GO:0019902 "phosphatase binding" evidence=IEA] [GO:0030100 "regulation of endocytosis" evidence=IEA] [GO:0032007 "negative regulation of TOR signaling" evidence=IEA] [GO:0033596 "TSC1-TSC2 complex" evidence=IEA] [GO:0042803 "protein homodimerization activity" evidence=IEA] [GO:0043491 "protein kinase B signaling" evidence=IEA] [GO:0046626 "regulation of insulin receptor signaling pathway" evidence=IEA] [GO:0048009 "insulin-like growth factor receptor signaling pathway" evidence=IEA] [GO:0048471 "perinuclear region of cytoplasm" evidence=IEA] [GO:0050918 "positive chemotaxis" evidence=IEA] [GO:0051056 "regulation of small GTPase mediated signal transduction" evidence=IEA] [GO:0051898 "negative regulation of protein kinase B signaling" evidence=IEA] [GO:0071889 "14-3-3 protein binding" evidence=IEA] InterPro:IPR000331 InterPro:IPR003913 InterPro:IPR016024 InterPro:IPR018515 InterPro:IPR027107 Pfam:PF02145 Pfam:PF03542 PRINTS:PR01431 PROSITE:PS50085 GO:GO:0005634 GO:GO:0005794 GO:GO:0048471 GO:GO:0008285 GO:GO:0007507 SUPFAM:SSF48371 GO:GO:0016020 Gene3D:1.25.10.10 InterPro:IPR011989 GO:GO:0005096 GO:GO:0006469 GO:GO:0014065 GO:GO:0001666 GO:GO:0051056 GO:GO:0032007 GO:GO:0030100 GO:GO:0006606 GO:GO:0001843 GO:GO:0048009 GO:GO:0043491 GO:GO:0050918 GO:GO:0051898 GO:GO:0014067 GO:GO:0046626 TreeFam:TF324484 PANTHER:PTHR10063 GeneTree:ENSGT00390000017866 GO:GO:0033596 OMA:VDKHRCD InterPro:IPR024584 PANTHER:PTHR10063:SF1 Pfam:PF11864 EMBL:AAEX03004629 EMBL:AAEX03004628 Ensembl:ENSCAFT00000030906 Uniprot:F1Q054)

HSP 1 Score: 473.396 bits (1217), Expect = 2.438e-137
Identity = 308/1039 (29.64%), Postives = 520/1039 (50.05%), Query Frame = 0
Query:   50 LQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRND-SPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIH------------------------AAKSKWLNIFSELV---ERYYKKE----------------KRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAER--DSLLK-----------------------DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACL-----LVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSA-------ARRPNVAL-------AAKKDVAADVPKXTQI-------------------------HPLA--------FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTML 967
            +  R++++  + E  +  + ++  VE L     DLL+ +  P+ RH VL+  +++V GQ  RLG++R+ FF  IK+  +     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YLD + + +++ +I  LC  T    + +  L+VL++++CY+ +P   L LFI  LCR +N++   +  W + R L+GTHLGHSA+Y +  I++      D  L+RGA+FF+G +LWG +R+ SL  S  ++LP+F  A+ C + +V +E++  + +L+  Y +EL A   D ++ ++E LL +++ L+      + + +H ++T +E L +   + GS+++ F +++ CAD RPE S++ LIT+R +SIH                        A + K L++ S ++    ++Y++E                K   +R     +++D+ +  H      L+D  I+  + +  + P   +  R + V +      +T   L LL IL   +       A R  ++L+                        D L+++  D  + LGL   DG+  FSPY +C + E E  +E       + AS P SP         +   V L          ++++CL+ E DW VL LVL+++P  L+ + LI     S+++  + L S           L  TP  FS++D H  + PVL AL SY+ +L    Q  ++  LE GL+ + C   C+VAL   ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV +I   L SN+ + F++  + KDS  R RSTSL+        AR P   L         K+  AA+  +   I                         + +A         H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L
Sbjct:   54 INNRIRVIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFRAIKDYPANE---DLHERLEVFKALTDNGRHITYLEEELAGFVLQWM-----DIGLSSEFLLVLVNLVKFNSCYLD-EYIASMVHMICLLCVQTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRRELQAVTWDILLNIMERLLHQLQSLDSP---ELRAIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNESRSAVRIKVLDVLSFVLLINRQFYEEELISSVVISQLSHVPEDKDPQVRKLATQLLVDLAEGCHTHHFNSLLD--IVEKVIARSLSPPPELEERDVAVYSASLEDVKT-AVLGLLVILQTKLYALPASHAMRVYETLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPSKDGAVRFSPYCVCDAMEPERGSE-------KKASGPLSPPTGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKLEVDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSGPRTLE-RLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYR-CASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPE-KDSF-RARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKL 1066          

HSP 2 Score: 242.276 bits (617), Expect = 5.021e-63
Identity = 136/343 (39.65%), Postives = 198/343 (57.73%), Query Frame = 0
Query: 1202 KKNQNNIPLNSSPNSSFESNL-NEPAMRTTRSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSN-KITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELK----EPLGHVMS---LSILVRQWALHCNLAAQIQKSLSNKMP-YSSNWLZXLRAIKRLKEKLQKE 1534
            ++NQ   P  + P     S+    P+    R +TIS S+P  R            V R            +KV GI+P FVFL LY S  FG+ ++  KP+LLP+  +  E S++ LD+IP+++THKI VLY+G  Q + E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ +FH+ATLMP  + D     KKRH+GN++ +IVYNDS    E +K+GT+RG+F    ++ITPLD   N V + C+ +++      +  ++S   L  + RQ ALH N+A+Q+  S SN    Y S W+  LR IKRL+ ++++E
Sbjct: 1437 EENQGQGP--AQPEGPLPSSCPRSPSGLRPRGYTISDSAPSRR---------GKRVERDAFRSRAGASNTEKVPGINPSFVFLQLYHSPFFGDESN--KPILLPNELQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGKLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKEVDKHRCDKKRHLGNDFVSIVYNDS---GEDFKLGTIRGQFNFVHVIITPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRHRIREE 1763          
BLAST of EMLSAG00000002599 vs. C. finmarchicus
Match: gi|592925097|gb|GAXK01033318.1| (TSA: Calanus finmarchicus comp83597_c1_seq1 transcribed RNA sequence)

HSP 1 Score: 422.935 bits (1086), Expect = 1.623e-124
Identity = 240/606 (39.60%), Postives = 338/606 (55.78%), Query Frame = 0
Query:  534 LLNILGCIMKRS--LEQEAE-RDSLLKDLLIVVAEDESYHLGLEESDG---SXXFSPYIICRSPXXXXXXXLLWNRQQEXXXXXXXXXXXXGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVS-FTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEEND------------KNKDSMGRMRSTSL-----SAARRPNXXXXXXXXXXXXXPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIR-DGTLSKDDITKNSDRRRHMSDFYSGTNKSQLSN--------LRSDKELHRHTSLERMESSGDDYRVKKVLPE------TCSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNL 1100
            L+N+L  + +R   LE   E R  + +  L + A +  YHLGL +  G      FSP+++C+            N+ +               +   V  +SL +ACL V+ CL+ E DW VL LVL +VP  LQN+ ++ RYGK I+KF   L S FT  +   PL   NTP KF + +F + ++PVLAALASYN  L    Q  LI+  E GLL+ +CN++CIVALT   LEM  S   L+PEVLLN SKIS+T  I+ P+LEFLST++ LP+VF+ F    +++VFAI LP+TNPFKF+ YTVSLAHHVI+MWFLKCRL+ R++FV+FII  L SN+  PFEE +             N+DS  R RS+SL     S   R N  + ++  +        +   L FHQELTETC+D++ARY + N  V  +R   + ++L  G S +WL+G ++IT++ S C     R G CD CY YC + DG  + + + +   ++RH S+      +  +          +R D  + +     R E  G   R +  LP+       C CWC GW E+ +RRPSG  SW+ RVQN  L +  +  T+L
Sbjct:  187 LVNVLDSVYERPAVLEHTGEVRLEIFRTFLSIRA-NRDYHLGLPKEGGRGEDYSFSPFVVCQ------------NKDE--------------GKVDGVTEMSLTRACLCVLRCLKEEKDWRVLGLVLDKVPRTLQNKGMLTRYGKDISKFAAVLCSLFTPSH---PLPNINTPPKFGRPEFQSAVYPVLAALASYNRSLEQDLQKKLIKCFEYGLLTSKCNQVCIVALTACILEMSGSMYTLLPEVLLNLSKISSTVHIAIPVLEFLSTLISLPKVFASFNTEQFLSVFAITLPYTNPFKFNHYTVSLAHHVIIMWFLKCRLATRKDFVKFIIKGLGSNVLQPFEEGNFRKVESGSSLASLNQDSSNRQRSSSLKEETTSRRVRHNTGVPSRPTINRLSGPDERQALLTFHQELTETCVDLMARYSFANCGVAPKRGPVAQYLLAQGQSNSWLLGNVIITITVSGCAQSGNRGGFCDSCYAYCRQEDGGENSEGVVEEGGKKRHQSEQSQRVRRELVGGEVEVVQPLVRDDSHVAQLKDDIRKEGGG---RKRGQLPDIPQSVGGCGCWCQGWCEVMVRRPSGVTSWVARVQNGLLSNQPSQETDL 1905          

HSP 2 Score: 290.812 bits (743), Expect = 6.779e-80
Identity = 173/356 (48.60%), Postives = 230/356 (64.61%), Query Frame = 0
Query: 1219 ESNLNEPA-MRTTRSHTISVSSPRNRVLNKPLQPTTXXXXXXXXXXXXXXEKNDKVSG--ISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHVMS--------LSILVRQWALHCNLAAQI-QKSLSNKM-PYSSNWLZXLRAIKRLXXXXXXXXXXXXXXXXXQGSGRPQLNDFTEFVIM 1561
            E  ++ PA +  TR+ TIS +SP     + PL P                  +D+  G  ISPQF+FL LYQ++    + + EKPLLLP+ K  E+S++NLDRI  +ETHK+GVLY+GP Q NDE  IL N  GSSRY+ FL GLG L++I   D +  F+GGLD  + DG F Y+W DD MQ ++H ATLMP +++DPQ NKKKRHIGN+Y AIVYNDS    ++Y MGTV+G+FI A I++ PLD GSNR+ V CK EL+  LGH+ +        LS +VRQ ALHCNLAA I  KS   K+ PY+SNWL+ LR IKR       + ++ +E++ E+ SG  Q++DFT +V M
Sbjct: 2101 EDAVDSPAPIPRTRAATIS-TSPTKLPRSAPLPPA----------------PSDRGGGAAISPQFLFLQLYQAAGIPTSYT-EKPLLLPATKSIESSLRNLDRIFCYETHKVGVLYVGPGQQNDEKAILSNQFGSSRYSAFLSGLGTLLDITTTDPNQVFLGGLDPRD-DGQFHYVWQDDAMQVVYHAATLMPRNESDPQFNKKKRHIGNDYVAIVYNDS---GDTYAMGTVKGQFIYAHIIVEPLDFGSNRISVVCKQELEGELGHLSTSRIVSDPNLSRVVRQIALHCNLAAIIFSKSKMTKVDPYASNWLERLRHIKRF------KGKVLKEQEEEETSG--QIHDFTGYV*M 3078          
BLAST of EMLSAG00000002599 vs. C. finmarchicus
Match: gi|592925091|gb|GAXK01033324.1| (TSA: Calanus finmarchicus comp83597_c9_seq1 transcribed RNA sequence)

HSP 1 Score: 422.935 bits (1086), Expect = 1.933e-124
Identity = 240/606 (39.60%), Postives = 338/606 (55.78%), Query Frame = 0
Query:  534 LLNILGCIMKRS--LEQEAE-RDSLLKDLLIVVAEDESYHLGLEESDG---SXXFSPYIICRSPXXXXXXXLLWNRQQEXXXXXXXXXXXXGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVS-FTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEEND------------KNKDSMGRMRSTSL-----SAARRPNXXXXXXXXXXXXXPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIR-DGTLSKDDITKNSDRRRHMSDFYSGTNKSQLSN--------LRSDKELHRHTSLERMESSGDDYRVKKVLPE------TCSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNL 1100
            L+N+L  + +R   LE   E R  + +  L + A +  YHLGL +  G      FSP+++C+            N+ +               +   V  +SL +ACL V+ CL+ E DW VL LVL +VP  LQN+ ++ RYGK I+KF   L S FT  +   PL   NTP KF + +F + ++PVLAALASYN  L    Q  LI+  E GLL+ +CN++CIVALT   LEM  S   L+PEVLLN SKIS+T  I+ P+LEFLST++ LP+VF+ F    +++VFAI LP+TNPFKF+ YTVSLAHHVI+MWFLKCRL+ R++FV+FII  L SN+  PFEE +             N+DS  R RS+SL     S   R N  + ++  +        +   L FHQELTETC+D++ARY + N  V  +R   + ++L  G S +WL+G ++IT++ S C     R G CD CY YC + DG  + + + +   ++RH S+      +  +          +R D  + +     R E  G   R +  LP+       C CWC GW E+ +RRPSG  SW+ RVQN  L +  +  T+L
Sbjct: 1427 LVNVLDSVYERPAVLEHTGEVRLEIFRTFLSIRA-NRDYHLGLPKEGGRGEDYSFSPFVVCQ------------NKDE--------------GKVDGVTEMSLTRACLCVLRCLKEEKDWRVLGLVLDKVPRTLQNKGMLTRYGKDISKFAAVLCSLFTPSH---PLPNINTPPKFGRPEFQSAVYPVLAALASYNRSLEQDLQKKLIKCFEYGLLTSKCNQVCIVALTACILEMSGSMYTLLPEVLLNLSKISSTVHIAIPVLEFLSTLISLPKVFASFNTEQFLSVFAITLPYTNPFKFNHYTVSLAHHVIIMWFLKCRLATRKDFVKFIIKGLGSNVLQPFEEGNFRKVESGSSLASLNQDSSNRQRSSSLKEETTSRRVRHNTGVPSRPTINRLSGPDERQALLTFHQELTETCVDLMARYSFANCGVAPKRGPVAQYLLAQGQSNSWLLGNVIITITVSGCAQSGNRGGFCDSCYAYCRQEDGGENSEGVVEEGGKKRHQSEQSQRVRRELVGGEVEVVQPLVRDDSHVAQLKDDIRKEGGG---RKRGQLPDIPQSVGGCGCWCQGWCEVMVRRPSGVTSWVARVQNGLLSNQPSQETDL 3145          

HSP 2 Score: 291.197 bits (744), Expect = 7.404e-80
Identity = 173/356 (48.60%), Postives = 230/356 (64.61%), Query Frame = 0
Query: 1219 ESNLNEPA-MRTTRSHTISVSSPRNRVLNKPLQPTTXXXXXXXXXXXXXXEKNDKVSG--ISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHVMS--------LSILVRQWALHCNLAAQI-QKSLSNKM-PYSSNWLZXLRAIKRLXXXXXXXXXXXXXXXXXQGSGRPQLNDFTEFVIM 1561
            E  ++ PA +  TR+ TIS +SP     + PL P                  +D+  G  ISPQF+FL LYQ++    + + EKPLLLP+ K  E+S++NLDRI  +ETHK+GVLY+GP Q NDE  IL N  GSSRY+ FL GLG L++I   D +  F+GGLD  + DG F Y+W DD MQ ++H ATLMP +++DPQ NKKKRHIGN+Y AIVYNDS    ++Y MGTV+G+FI A I++ PLD GSNR+ V CK EL+  LGH+ +        LS +VRQ ALHCNLAA I  KS   K+ PY+SNWL+ LR IKR       + ++ +E++ E+ SG  Q++DFT +V M
Sbjct:  254 EDAVDSPAPIPRTRAATIS-TSPTKLPRSAPLPPA----------------PSDRGGGAAISPQFLFLQLYQAAGIPTSYT-EKPLLLPATKSIESSLRNLDRIFCYETHKVGVLYVGPGQQNDEKAILSNQFGSSRYSAFLSGLGTLLDITTTDPNQVFLGGLDPRD-DGQFHYVWQDDAMQVVYHAATLMPRNESDPQFNKKKRHIGNDYVAIVYNDS---GDTYAMGTVKGQFIYAHIIVEPLDFGSNRISVVCKQELEGELGHLSTSRIVSDPNLSRVVRQIALHCNLAAIIFSKSKMTKVDPYASNWLERLRHIKRF------KGKVLKEQEEEETSG--QIHDFTGYV*M 1231          
BLAST of EMLSAG00000002599 vs. C. finmarchicus
Match: gi|592925096|gb|GAXK01033319.1| (TSA: Calanus finmarchicus comp83597_c1_seq2 transcribed RNA sequence)

HSP 1 Score: 288.886 bits (738), Expect = 4.580e-82
Identity = 175/356 (49.16%), Postives = 227/356 (63.76%), Query Frame = 0
Query: 1219 ESNLNEPA-MRTTRSHTISVSSPRNRVLNKPLQPTTXXXXXXXXXXXXXXEKNDKVSG--ISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHVMS--------LSILVRQWALHCNLAAQI-QKSLSNKM-PYSSNWLZXLRAIKRLXXXXXXXXXXXXXXXXXQGSGRPQLNDFTEFVIM 1561
            E  ++ PA +  TR+ TIS +SP     + PL P                  +D+  G  ISPQF+FL LYQ++    + + EKPLLLP+ K  E+S++NLDRI  +ETHK+GVLY+GP Q NDE  IL N  GSSRY+ FL GLG L++I   D +  F+GGLD  + DG F Y+W DD MQ ++H ATLMP +++DPQ NKKKRHIGN+Y AIVYNDS    ++Y MGTV+G+FI A I++ PLD GSNR+ V CK EL+  LGH+ +        LS +VRQ ALHCNLAA I  KS   K+ PY+SNWL+ LR IKR K K+ KE E +E           Q++DFT +V M
Sbjct:  778 EDAVDSPAPIPRTRAATIS-TSPTKLPRSAPLPPA----------------PSDRGGGAAISPQFLFLQLYQAAGIPTSYT-EKPLLLPATKSIESSLRNLDRIFCYETHKVGVLYVGPGQQNDEKAILSNQFGSSRYSAFLSGLGTLLDITTTDPNQVFLGGLDPRD-DGQFHYVWQDDAMQVVYHAATLMPRNESDPQFNKKKRHIGNDYVAIVYNDS---GDTYAMGTVKGQFIYAHIIVEPLDFGSNRISVVCKQELEGELGHLSTSRIVSDPNLSRVVRQIALHCNLAAIIFSKSKMTKVDPYASNWLERLRHIKRFKGKVLKEQEEEETSG--------QIHDFTGYV*M 1755          

HSP 2 Score: 141.354 bits (355), Expect = 1.975e-33
Identity = 68/197 (34.52%), Postives = 103/197 (52.28%), Query Frame = 0
Query:  919 FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIR-DGTLSKDDITKNSDRRRHMSDFYSGTNKSQLSN--------LRSDKELHRHTSLERMESSGDDYRVKKVLPE------TCSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNL 1100
            FHQELTETC+D++ARY + N  V  +R   + ++L  G S +WL+G ++IT++ S C     R G CD CY YC + DG  + + + +   ++RH S+      +  +          +R D  + +     R E  G   R +  LP+       C CWC GW E+ +RRPSG  SW+ RVQN  L +  +  T+L
Sbjct:    1 FHQELTETCVDLMARYSFANCGVAPKRGPVAQYLLAQGQSNSWLLGNVIITITVSGCAQSGNRGGFCDSCYAYCRQEDGGENSEGVVEEGGKKRHQSEQSQRVRRELVGGEVEVVQPLVRDDSHVAQLKDDIRKEGGG---RKRGQLPDIPQSVGGCGCWCQGWCEVMVRRPSGVTSWVARVQNGLLSNQPSQETDL 582          
BLAST of EMLSAG00000002599 vs. C. finmarchicus
Match: gi|592925093|gb|GAXK01033322.1| (TSA: Calanus finmarchicus comp83597_c1_seq5 transcribed RNA sequence)

HSP 1 Score: 118.627 bits (296), Expect = 1.065e-28
Identity = 67/157 (42.68%), Postives = 101/157 (64.33%), Query Frame = 0
Query:  277 LYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIXXXXXXXXXXXXXXXXXXQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRL 433
            LY L  I+Q  ENR + AL+RGA+FFIG SLWG  RI SL YS +T+LP F  AL   H LV++EV+ QV++LVT + + + A + +  V ++ELL+  +  LE  ++  + +HL A I  LE L+    Y+GS+ +L+ +++  +D  PE SV+ L
Sbjct:    2 LYQLCQIIQNPENREETALVRGAVFFIGHSLWGAQRIPSLKYSPMTVLPVFCTALSTSHQLVLYEVVVQVERLVTKHQETITAPSWEEAVSVLELLVTRLGILEDDMKQLVHTHLQATIGQLEQLAMTDSYAGSRSRLYSLVESVSDISPEASVVHL 472          
BLAST of EMLSAG00000002599 vs. C. finmarchicus
Match: gi|592925092|gb|GAXK01033323.1| (TSA: Calanus finmarchicus comp83597_c1_seq6 transcribed RNA sequence)

HSP 1 Score: 110.153 bits (274), Expect = 5.958e-26
Identity = 62/149 (41.61%), Postives = 96/149 (64.43%), Query Frame = 0
Query:  285 QTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIXXXXXXXXXXXXXXXXXXQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRL 433
            Q  +NR + AL+RGA+FFIG SLWG  RI SL YS +T+LP F  AL   H LV++EV+ QV++LVT + + + A + +  V ++ELL+  +  LE  ++  + +HL A I  LE L+    Y+GS+ +L+ +++  +D  PE SV+ L
Sbjct:    1 QNPDNREETALVRGAVFFIGHSLWGAQRIPSLKYSPMTVLPVFCTALSTSHQLVLYEVVVQVERLVTKHQETITAPSWEEAVSVLELLVTRLGILEDDMKQLVHTHLQATIGQLEQLAMTDSYAGSRSRLYSLVESVSDISPEASVVHL 447          
BLAST of EMLSAG00000002599 vs. C. finmarchicus
Match: gi|592925088|gb|GAXK01033327.1| (TSA: Calanus finmarchicus comp83597_c9_seq4 transcribed RNA sequence)

HSP 1 Score: 106.686 bits (265), Expect = 4.546e-25
Identity = 51/88 (57.95%), Postives = 63/88 (71.59%), Query Frame = 0
Query:  813 TVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEEND------------KNKDSMGRMRSTSL 888
            +VFAI LP+TNPFKF+ YTVSLAHHVI+MWFLKCRL+ R++FV+FII  L SN+  PFEE +             N+DS  R RS+SL
Sbjct:  105 SVFAITLPYTNPFKFNHYTVSLAHHVIIMWFLKCRLATRKDFVKFIIKGLGSNVLQPFEEGNFRKVESGSSLASLNQDSSNRQRSSSL 368          
BLAST of EMLSAG00000002599 vs. C. finmarchicus
Match: gi|592925090|gb|GAXK01033325.1| (TSA: Calanus finmarchicus comp83597_c9_seq2 transcribed RNA sequence)

HSP 1 Score: 105.531 bits (262), Expect = 1.425e-23
Identity = 71/195 (36.41%), Postives = 102/195 (52.31%), Query Frame = 0
Query:  534 LLNILGCIMKRS--LEQEAE-RDSLLKDLLIVVAEDESYHLGLEESDG---SXXFSPYIICRSPXXXXXXXLLWNRQQEXXXXXXXXXXXXGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVS-FTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASY 721
            L+N+L  + +R   LE   E R  + +  L + A +  YHLGL +  G      FSP+++C+            N+ +               +   V  +SL +ACL V+ CL+ E DW VL LVL +VP  LQN+ ++ RYGK I+KF   L S FT  +   PL   NTP KF + +F + ++PVLAALASY
Sbjct:    3 LVNVLDSVYERPAVLEHTGEVRLEIFRTFLSIRA-NRDYHLGLPKEGGRGEDYSFSPFVVCQ------------NKDE--------------GKVDGVTEMSLTRACLCVLRCLKEEKDWRVLGLVLDKVPRTLQNKGMLTRYGKDISKFAAVLCSLFTPSH---PLPNINTPPKFGRPEFQSAVYPVLAALASY 497          
BLAST of EMLSAG00000002599 vs. C. finmarchicus
Match: gi|592925094|gb|GAXK01033321.1| (TSA: Calanus finmarchicus comp83597_c1_seq4 transcribed RNA sequence)

HSP 1 Score: 105.531 bits (262), Expect = 1.425e-23
Identity = 71/195 (36.41%), Postives = 102/195 (52.31%), Query Frame = 0
Query:  534 LLNILGCIMKRS--LEQEAE-RDSLLKDLLIVVAEDESYHLGLEESDG---SXXFSPYIICRSPXXXXXXXLLWNRQQEXXXXXXXXXXXXGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVS-FTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASY 721
            L+N+L  + +R   LE   E R  + +  L + A +  YHLGL +  G      FSP+++C+            N+ +               +   V  +SL +ACL V+ CL+ E DW VL LVL +VP  LQN+ ++ RYGK I+KF   L S FT  +   PL   NTP KF + +F + ++PVLAALASY
Sbjct:  203 LVNVLDSVYERPAVLEHTGEVRLEIFRTFLSIRA-NRDYHLGLPKEGGRGEDYSFSPFVVCQ------------NKDE--------------GKVDGVTEMSLTRACLCVLRCLKEEKDWRVLGLVLDKVPRTLQNKGMLTRYGKDISKFAAVLCSLFTPSH---PLPNINTPPKFGRPEFQSAVYPVLAALASY 697          
BLAST of EMLSAG00000002599 vs. C. finmarchicus
Match: gi|592925095|gb|GAXK01033320.1| (TSA: Calanus finmarchicus comp83597_c1_seq3 transcribed RNA sequence)

HSP 1 Score: 105.531 bits (262), Expect = 1.425e-23
Identity = 71/195 (36.41%), Postives = 102/195 (52.31%), Query Frame = 0
Query:  534 LLNILGCIMKRS--LEQEAE-RDSLLKDLLIVVAEDESYHLGLEESDG---SXXFSPYIICRSPXXXXXXXLLWNRQQEXXXXXXXXXXXXGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVS-FTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASY 721
            L+N+L  + +R   LE   E R  + +  L + A +  YHLGL +  G      FSP+++C+            N+ +               +   V  +SL +ACL V+ CL+ E DW VL LVL +VP  LQN+ ++ RYGK I+KF   L S FT  +   PL   NTP KF + +F + ++PVLAALASY
Sbjct:  203 LVNVLDSVYERPAVLEHTGEVRLEIFRTFLSIRA-NRDYHLGLPKEGGRGEDYSFSPFVVCQ------------NKDE--------------GKVDGVTEMSLTRACLCVLRCLKEEKDWRVLGLVLDKVPRTLQNKGMLTRYGKDISKFAAVLCSLFTPSH---PLPNINTPPKFGRPEFQSAVYPVLAALASY 697          
BLAST of EMLSAG00000002599 vs. C. finmarchicus
Match: gi|592925089|gb|GAXK01033326.1| (TSA: Calanus finmarchicus comp83597_c9_seq3 transcribed RNA sequence)

HSP 1 Score: 94.3597 bits (233), Expect = 1.914e-20
Identity = 58/165 (35.15%), Postives = 89/165 (53.94%), Query Frame = 0
Query:   77 LLYKTKDLLRN-DSPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTL----KFLEMITNCFIFNATYLDSDVVLALITLISSL-CTSTNDKNETKSCLEVLNSIICYSYI 235
            L YK +D+L +    + RH V  F   LV GQY+ L ++R   F  I+E+   +   D    + LL  LT  GKDIV+ E+++GPF+ E +  +T           + L + +N   FN+ YL   VV+  I  ++ + CTSTNDK E   CL++L  ++ YSY+
Sbjct:    1 LWYKVQDMLESSQEKETRHTVFEFLVQLVVGQYNSLDMMRPQLFLFIREH---KVQEDMAMRMSLLVALTENGKDIVHIEEQVGPFLLELLELVTEGGDGGDEVVGQLLSITSNMVKFNSAYLSQAVVVRFIVKLAQISCTSTNDK-EILQCLDILKCVVMYSYV 483          
BLAST of EMLSAG00000002599 vs. L. salmonis peptides
Match: EMLSAP00000002599 (pep:novel supercontig:LSalAtl2s:LSalAtl2s151:403390:412004:-1 gene:EMLSAG00000002599 transcript:EMLSAT00000002599 description:"maker-LSalAtl2s151-augustus-gene-4.14")

HSP 1 Score: 3237.97 bits (8394), Expect = 0.000e+0
Identity = 1576/1576 (100.00%), Postives = 1576/1576 (100.00%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAERDSLLKDLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSAARRPNVALAAKKDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKDDITKNSDRRRHMSDFYSGTNKSQLSNLRSDKELHRHTSLERMESSGDDYRVKKVLPETCSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNLRELTALFDTSLKKDTFNFDVSTESDKINVDNLTEDEYKKLSSEFVPSSVSSPSAETDKTVSGNRSHDEEMIVMSPKSPVKKTKSSPPDTSCQPLEVSDIKRKKNQNNIPLNSSPNSSFESNLNEPAMRTTRSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHVMSLSILVRQWALHCNLAAQIQKSLSNKMPYSSNWLZXLRAIKRLKEKLQKENEMKEEEDREQGSGRPQLNDFTEFVIMRKKKGNGGSGNSVGD 1576
            MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAERDSLLKDLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSAARRPNVALAAKKDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKDDITKNSDRRRHMSDFYSGTNKSQLSNLRSDKELHRHTSLERMESSGDDYRVKKVLPETCSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNLRELTALFDTSLKKDTFNFDVSTESDKINVDNLTEDEYKKLSSEFVPSSVSSPSAETDKTVSGNRSHDEEMIVMSPKSPVKKTKSSPPDTSCQPLEVSDIKRKKNQNNIPLNSSPNSSFESNLNEPAMRTTRSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHVMSLSILVRQWALHCNLAAQIQKSLSNKMPYSSNWLZXLRAIKRLKEKLQKENEMKEEEDREQGSGRPQLNDFTEFVIMRKKKGNGGSGNSVGD
Sbjct:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAERDSLLKDLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSAARRPNVALAAKKDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKDDITKNSDRRRHMSDFYSGTNKSQLSNLRSDKELHRHTSLERMESSGDDYRVKKVLPETCSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNLRELTALFDTSLKKDTFNFDVSTESDKINVDNLTEDEYKKLSSEFVPSSVSSPSAETDKTVSGNRSHDEEMIVMSPKSPVKKTKSSPPDTSCQPLEVSDIKRKKNQNNIPLNSSPNSSFESNLNEPAMRTTRSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHVMSLSILVRQWALHCNLAAQIQKSLSNKMPYSSNWLZXLRAIKRLKEKLQKENEMKEEEDREQGSGRPQLNDFTEFVIMRKKKGNGGSGNSVGD 1576          
BLAST of EMLSAG00000002599 vs. L. salmonis peptides
Match: EMLSAP00000004974 (pep:novel supercontig:LSalAtl2s:LSalAtl2s260:1491039:1496975:-1 gene:EMLSAG00000004974 transcript:EMLSAT00000004974 description:"maker-LSalAtl2s260-augustus-gene-14.34")

HSP 1 Score: 87.0409 bits (214), Expect = 3.947e-17
Identity = 70/236 (29.66%), Postives = 117/236 (49.58%), Query Frame = 0
Query: 1313 IKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCK-------PELKEPLGHVMSLSILVRQWALHCNLAAQIQKSLSNKMPY-SSNWLZXLRAIKRLKEKLQKENEMKEE 1540
            +KNLD I   ETHKI ++Y+   Q  D+  IL N SGS  Y +F+ GL        ++  T F+ GL   +  G     +    ++ +FHVAT MP+   +    +K RH+GN+   IV+++   H   Y+ G +  +F    I+I PL     R+ +  K       P   E +    +L  LVR  A++   A++ ++S+   +PY  S++ +  +A++ +  K  KE+   EE
Sbjct: 1594 LKNLDNIRGRETHKIAIIYVAAGQ-EDKHSILDNSSGSQAYEEFISGLA---WXXELESHTGFMAGLXKNKSTGETXPYYATSFIEVMFHVATRMPSCTEE-SLLQKTRHLGNDEIHIVWSE---HYRDYRRGILPTEFCDVLIIIYPLPNKLFRIQISRKLEVPFFGPLFNESIVDGKALPGLVRATAIN---ASRAKRSM---IPYFQSHYEERAKALEDII-KNHKESTTYEE 1814          
BLAST of EMLSAG00000002599 vs. L. salmonis peptides
Match: EMLSAP00000009951 (pep:novel supercontig:LSalAtl2s:LSalAtl2s64:61995:90540:1 gene:EMLSAG00000009951 transcript:EMLSAT00000009951 description:"maker-LSalAtl2s64-augustus-gene-1.7")

HSP 1 Score: 76.2554 bits (186), Expect = 5.589e-14
Identity = 50/154 (32.47%), Postives = 81/154 (52.60%), Query Frame = 0
Query: 1325 HKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGD-GNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSN-RVCVDCKPEL 1476
            +K GV+Y    QV +E  +  N   S  + +FLD LGN + +    +   F GGLD   G  G+ +        + +FHV+TL+P  DNDPQ  ++KRHIGN+  A+V+ D       +    +   F+ A +V+ P+  GS+ +V V  + ++
Sbjct:  387 NKFGVIYQKQGQVTEEE-LFANREHSPAFEEFLDILGNRVLL---TEHKGFRGGLDTTYGQTGDVSVYETFHNREIMFHVSTLLPYTDNDPQQLQRKRHIGNDIVALVFQDDET---PFCPDMITSHFLHAYVVVRPIKDGSDYKVSVSARKDV 533          
BLAST of EMLSAG00000002599 vs. L. salmonis peptides
Match: EMLSAP00000009942 (pep:novel supercontig:LSalAtl2s:LSalAtl2s64:423747:425954:-1 gene:EMLSAG00000009942 transcript:EMLSAT00000009942 description:"augustus_masked-LSalAtl2s64-processed-gene-4.0")

HSP 1 Score: 74.7146 bits (182), Expect = 1.260e-13
Identity = 57/200 (28.50%), Postives = 91/200 (45.50%), Query Frame = 0
Query: 1270 NDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGD-GNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRV 1468
            +D VSG+SP  +   +    +         P+L PS       I   D       +K G++Y    Q  +E  +  N S       FL+ LG  + + +      + GGLD + G  G  +Y       + +FHV+TL+P  DNDPQ  ++KRHIGN+  AI++ +       +    +   F+ A IV+ P+   SN+V
Sbjct:  166 SDLVSGVSPLKIMNFVQPEHSVNQFV----PILCPS---ASELIMKYDEHLLVNHNKFGIVYQKVGQTTEE-ALFGNKSHCPAMETFLNLLGKKVMLXS---HKGYRGGLDTQYGQTGKHSYFETFQGREIMFHVSTLLPXXDNDPQQLQRKRHIGNDIVAIIFQEGET---PFSPDMITSHFLHAYIVVRPVQXSSNKV 351          
BLAST of EMLSAG00000002599 vs. SwissProt
Match: gi|9297076|sp|Q61037.1|TSC2_MOUSE (RecName: Full=Tuberin; AltName: Full=Tuberous sclerosis 2 protein homolog)

HSP 1 Score: 476.863 bits (1226), Expect = 1.635e-139
Identity = 324/1088 (29.78%), Postives = 541/1088 (49.72%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIR-----LSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRND-SPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKW------------------------LNI--FSELVERYYKKEKRVN-----------------IRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAER--DSLLK-----------------------DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSA-------ARRPNVAL-------AAKKDVAADVPKXTQI-------------------------HPLA--------FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTML 967
            M+K +  +    EK K       S   P   E +     ++ E++  +S     L  R++++  + +  +  +L++  VE L     DLL+ +  P+ RH VL+  +++V GQ  RLG++R+ FF +IK+  S     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YLD + + +++ +I  LC  T    + +  L+VL++++CY+ +P   L LFI  LCR +N++   +  W + R L+GTHLGHSA+Y +  I++      D  L+RGA+FF+G +LWG +R+ SL  S  ++LP+F  A+ C + +V +E++  + +L+  Y KEL A   D ++++IE LL +++ L+      + + +H ++T +E L +   + GS+++ + +++  AD RPE S++ LI++R +SIH AK  W                        L++  F  L+ R + +E+ +N                 +R     +++D+ +  H      L+D  II  + +  + P   +  R + V +      +T   L LL IL   +       A R  +SL+                        D L+++  D  + LGL   DG   FSPY +C   E +  +E   +      + P SP  +    +   L  SL    LL  +CL++E+DW VL LVL+R+P  L+ + LI     S+++  + L S           L  TP  FS++D H  + PVL AL SY+ +L    Q  ++  LE GL+ + C   C+VAL   ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV +I   L SN+ + F++  + KDS  R RSTSL+        AR P   L         K+  AA+  +   I                         + +A         H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L
Sbjct:    1 MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITSEILRELSGECG-LNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLTLLKAIVQGQGDRLGVLRALFFKVIKDYPSNE---DLHERLEVFKALTDNGRHITYLEEELAEFVLQWM-----DVGLSSEFLLVLVNLVKFNSCYLD-EYIASMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMEDRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSP---ELKTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLISYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVAIKVLDVLSFVLLIIRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLD--IIEKVMARSLSPPPELEERDLAVHSASLEDVKT-AVLGLLVILQTKLYTLPASHATRVYESLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCMELDRASEKKASGPLSPPTGPPSPVPMGPAVRLGYLPYSLLFRVLL--QCLKQESDWKVLKLVLSRLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLE-RLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYR-CASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFVPEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPE-KDSF-RARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKL 1066          

HSP 2 Score: 224.557 bits (571), Expect = 8.976e-58
Identity = 127/321 (39.56%), Postives = 186/321 (57.94%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVR-GKFISACIVITPLDQGSNRVCVDCKPE--------LKEP-------LGHVMSLSILVRQWALHCNLAAQIQKSLSNKMP-YSSNWLZXLRAIKRLKEKLQKE 1534
            R +TIS S+P  R            V R       +    +KV GI+P FVFL LY S  FG+ ++  KP+LLP N+  E S++ LD+IP+++THKI VLY+G  Q + E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ +FH+ATLMP  D D     KKRH+GN++ +I+YNDS    E +K+GT++ G+F    ++ITPLD   N + + C+ +         ++P       L  VM L++ +     H  +A+Q+  S SN    Y S W+  LR IKRL++++++E
Sbjct: 1462 RGYTISDSAPSRR---------GKRVERDNFKSRAAASSAEKVPGINPSFVFLQLYHSPFFGDESN--KPILLP-NESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDS---GEDFKLGTIKQGQFNFVHVIITPLDYKCNLLTLQCRKDGPACKCEWWRQPGEIVVWALPVVMELTVTI--LLCHLQMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRIREE 1765          
BLAST of EMLSAG00000002599 vs. SwissProt
Match: gi|1717800|sp|P49816.1|TSC2_RAT (RecName: Full=Tuberin; AltName: Full=Tuberous sclerosis 2 protein homolog)

HSP 1 Score: 476.478 bits (1225), Expect = 2.218e-139
Identity = 323/1093 (29.55%), Postives = 540/1093 (49.41%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIR-----LSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRND-SPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKW------------------------LNI--FSELVERYYKKEKRVN-----------------IRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAER--DSLLK-----------------------DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPE-----EEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSA-------ARRPNVAL-------AAKKDVAADVPKXTQI-------------------------HPLA--------FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTML 967
            M+K +  +    EK K       S   P   E +     ++ E++  +S     L  R++++  + +  +  +L++  VE L     DLL+ +  P+ RH VL+  +++V GQ  RLG++R+ FF +IK+  S     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YLD + +  ++ +I  LC  T    + +  L+VL++++CY+ +P   L LFI  LCR VN++   +  W + R L+GTHLGHSA+Y +  I++      D  L+RGA+FF+G +LWG +R+ SL  S  ++LP+F  A+ C + +V +E++  + +L+  Y KEL A   D ++++IE LL +++ L+      + + +H ++T +E L +   + GS+++ + +++  AD RPE S++ LIT+R +SIH AK  W                        L++  F  L+ R + +E+ +N                 +R     +++D+ +  H      L+D  II  + +  + P   +  R + V +      +T   L LL IL   +       A R  ++L+                        D L+++  D  + LGL   DG   FSPY +C   E     E++ +  L       +  P+ P+   G    S+L   L       ++CL++ETDW VL LVL+++P  L+ + LI     S+++  + L S           L  TP  FS++D H  + PVL AL SY+ +L    Q  ++  LE GL+ + C   C+VAL   ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV +I   L SN+ + F++  + KD   R RSTSL+        AR P   L         K+  AA+  +   I                         + +A         H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L
Sbjct:    1 MAKPTSKDSGLKEKFKILLGLGTSRPNPRCAEGKQTEFIITAEILRELSGECG-LNNRIRMIGQICDVAKTKKLEEHAVEALWKAVSDLLQPERPPEARHAVLALLKAIVQGQGDRLGVLRALFFKVIKDYPSNE---DLHERLEVFKALTDNGRHITYLEEELAEFVLQWM-----DVGLSSEFLLVLVNLVKFNSCYLD-EYIAPMVHMICLLCIRTVSSVDIEVSLQVLDAVVCYNCLPAESLPLFIITLCRTVNVKELCEPCWKLMRNLLGTHLGHSAIYNMCRIMENRSYMEDAPLLRGAVFFVGMALWGAHRLYSLKNSPTSVLPSFYEAMTCPNEVVSYEIVLSITRLIKKYRKELQAVTWDILLDIIERLLQQLQNLDSP---ELRTIVHDLLTTVEELCDQNEFHGSQERYYELVESYADQRPESSLLNLITYRAQSIHPAKDGWIQNLQLLMERFFRNECRSAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLD--IIEKVMARSLSPPLELEERDLAVYSASLEDVKT-AVLGLLVILQTKLYTLPASHATRVYETLISHIQLHYKHGYSLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCLCDCAELDRASEKKASGPLSPPTGPPSPVPTGPAVRLGHLPYSLLFRVL-------LQCLKQETDWKVLKLVLSKLPESLRYKVLIFTSPCSVDQLSSALCSMLSAPKTLE-RLRGTPEGFSRTDLHLAVVPVLTALISYHNYLDKTRQREMVYCLEQGLIYR-CASQCVVALAICSVEMPDIIIKALPVLVVKLTHISATASMAIPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPYITKGLRSNVLLSFDDTPE-KDKF-RARSTSLNERPKSLRIARAPKQGLNNSPPVKEFKESCAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSMAQADDNLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKL 1066          

HSP 2 Score: 238.039 bits (606), Expect = 7.135e-62
Identity = 128/312 (41.03%), Postives = 188/312 (60.26%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELK----EPLGHVMS---LSILVRQWALHCNLAAQIQKSLSNKMP-YSSNWLZXLRAIKRLKEKLQKE 1534
            R +TIS S+P  R            V R       +    +KV GI+P FVFL LY S   G+ ++  KP+LLP N+  E S++ LD+IP+++THKI VLY+G  Q + E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ +FH+ATLMP  D D     KKRH+GN++ +I+YNDS    E +K+GT++G+F    ++ITPLD   N + + C+ +++      +  ++S   LS + RQ ALH N+A+Q+    SN    Y S W+  LR IKRL++++++E
Sbjct: 1463 RGYTISDSAPSRR---------GKRVERDNFKSRTAASSAEKVPGINPSFVFLQLYHSPFCGDESN--KPILLP-NESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDS---GEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQVHHRRSNPTDIYPSKWIARLRHIKRLRQRIREE 1759          
BLAST of EMLSAG00000002599 vs. SwissProt
Match: gi|269849475|sp|P49815.2|TSC2_HUMAN (RecName: Full=Tuberin; AltName: Full=Tuberous sclerosis 2 protein)

HSP 1 Score: 475.707 bits (1223), Expect = 3.390e-139
Identity = 311/1039 (29.93%), Postives = 515/1039 (49.57%), Query Frame = 0
Query:   50 LQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIH------------------------AAKSKWLNI--FSELVERYYKKEKRVN-----------------IRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAER--DSLLK-----------------------DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACL-----LVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSA-------ARRPNVAL-------AAKKDVAADVPKXTQI----HPLA-----------------------------FHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTML 967
            L  R++++  + E  +  + ++  VE L     DLL+ + P + RH VL+  +++V GQ  RLG++R+ FF +IK+  S     D    +++   LT  G+ I Y E+EL  F+ + +      +  + +FL ++ N   FN+ YLD + +  ++ +I  LC  T    + +  L+VL++++CY+ +P   L LFI  LCR +N++   +  W + R L+GTHLGHSA+Y +  +++      D  L+RGA+FF+G +LWG +R+ SL  S  ++LP+F  A+ C + +V +E++  + +L+  Y KEL   A D ++ +IE LL +++ L+      + + +H ++T +E L +   + GS+++ F +++ CAD RPE S++ LI++R +SIH                        A + K L++  F  L+ R + +E+ +N                 +R     +++D+ +  H      L+D  II  + +  + P   +  R +   +      +T   L LL IL   +       A R  + L+                        D L+++  D  + LGL   DG   FSPY +C   E E  +E       +  S P SP         +   V L          ++++CL++E+DW VL LVL R+P  L+ + LI     S+++  + L S           L   P  FS++D H  + PVL AL SY+ +L    Q  ++  LE GL+ + C   C+VAL+  ++EM       +P +++  + ISAT  ++ P+LEFLST+ RLP ++  F    Y +VFAI+LP+TNP KF+ Y V LAHHVI MWF++CRL +R++FV FI   L SN+ + F++  + KDS  R RSTSL+        AR P   L         K+  AA+  +   I    H +                               H ELTETCLD++ARY++ N +   +R    +F+L  G + TWL+G  L
Sbjct:   54 LNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNE---DLHERLEVFKALTDNGRHITYLEEELADFVLQWM-----DVGLSSEFLLVLVNLVKFNSCYLD-EYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSP---ELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLD--IIEKVMARSLSPPPELEERDVAAYSASLEDVKT-AVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSE-------KKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLE-RLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHR-CASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPE-KDSF-RARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKL 1066          

HSP 2 Score: 243.817 bits (621), Expect = 1.240e-63
Identity = 130/313 (41.53%), Postives = 189/313 (60.38%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKIT-ENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELK----EPLGHVMS---LSILVRQWALHCNLAAQIQKSLSNKMP-YSSNWLZXLRAIKRLKEKLQKE 1534
            R +TIS S+P  R            V R  +    +    +KV GI+P FVFL LY S  FG+ ++  KP+LLP+   + E S++ LD+IP+++THKI VLY+G  Q N E+ IL N  GS RYT+FL GLG LI +++      ++GGLD    DG F Y W DD+MQ +FH+ATLMP  D D     KKRH+GN++ +IVYNDS    E +K+GT++G+F    +++TPLD   N V + C+ +++      +  ++S   L  + RQ ALH N+A+Q+  S SN    Y S W+  LR IKRL++++ +E
Sbjct: 1459 RGYTISDSAPSRR---------GKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESN--KPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDS---GEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEE 1757          
BLAST of EMLSAG00000002599 vs. SwissProt
Match: gi|62287828|sp|Q9UUG9.1|TSC2_SCHPO (RecName: Full=Tuberous sclerosis 2 protein homolog)

HSP 1 Score: 133.265 bits (334), Expect = 7.094e-30
Identity = 93/283 (32.86%), Postives = 137/283 (48.41%), Query Frame = 0
Query: 1299 KPLLLPSNKIT-ENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEG-DGNFAYMWXDDVMQXIFHVATLMP-NHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVC---------------VDCKPELKEPLGHVMSLSILVRQWALHCNLAAQIQKSLSNKMPYSSNWLZXLRAIKRLKEKLQK----ENEMKEEEDREQGSGRPQLNDFTEFV 1559
            +PLLLPSN  +   +I   DRIP  E+ K G++Y+G  Q   E  IL N + S  +  FL+GLG L  ++   K   F GGLD E   DG FAY W D V Q +FH  T+MP N ++DP    KKRHIGN++  I++N+S      Y   T+  +F    IVITP  +   R                 +D     +  +    +L  +VR   L+  + + I    +    Y   W + LR +KRL+EK Q     E+   +E+ + +       +DFT ++
Sbjct: 1065 QPLLLPSNDDSVRRAISVFDRIPVIESLKAGLVYVGYQQ-RREADILANTNPSEDFLTFLNGLGTLFELKTDQKV--FAGGLDRENDIDGAFAYCWKDKVTQMVFHCTTMMPTNIEHDPGCTLKKRHIGNDFVTIIFNES---GLEYDFDTIPSQFNFVNIVITPESESIRRTGRQIKFYKVKALTKYDIDFSLFRRYKIVSSDALPAIVRDVTLNAAVFSHIYHRSAGD--YVHIWAERLRQLKRLREKFQASVLPEDYNLDEQTKTKLQNGTNFSDFTSYL 1339          

HSP 2 Score: 81.6481 bits (200), Expect = 3.883e-14
Identity = 86/398 (21.61%), Postives = 160/398 (40.20%), Query Frame = 0
Query:  600 LWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSAL---EMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALP------FTNPFK------------FDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSAARRPNVALAAKKDVAADVPKXTQIHPLAFHQELTETCL---DILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTS 973
            +W R++    + S+ S   G   K  +    +       + +  E++W +L  ++T +   + NR +     + I   +  +     +  +    +   PS   K++    I   +  L  Y++    A +  L+     GL  ++ N  C V++ +  L   E+  S R  +P +L+  S++     +S  ILEFL ++ RLP++ + FTD +Y  +FAIAL       FT   K            +  Y ++LA+ V+ +WFL  RL+ R+ FV +I                        +R   L++  +P                            L + CL   D++ ++ Y N  +     +TS F+  +  S TW+ G  L T++ S
Sbjct:  663 IWERKENDLIKISNHSTEYG---KDFVTFPTSSLLRFYRKSMATESNWTILMFMITHLADQISNRSMFIGALEEIYNLLDFMCDIVFERVSISAEI---PSNIRKANIMIPILQNVQMLFVYHDQFSRAQEDELVSVFFAGL--QKWNEACHVSIHSLMLCCYELPVSIRKQLPAILVTLSRLITKPDLSVHILEFLCSLARLPDLIANFTDADYRQIFAIALKYIQHRDFTKESKDSNDTESILKNSYSSYVLALAYSVLQIWFLSLRLTERKKFVPWI------------------------LRGLKLASEGKP----------------------------LEDLCLVQYDMMQQFCYSNSDINN---QTSTFVDSDVESETWIRGNSLFTINVS 997          
BLAST of EMLSAG00000002599 vs. SwissProt
Match: gi|284022067|sp|P86411.1|RGPA2_RAT (RecName: Full=Ral GTPase-activating protein subunit alpha-2; AltName: Full=250 kDa substrate of Akt; Short=AS250; AltName: Full=p220)

HSP 1 Score: 103.605 bits (257), Expect = 9.851e-21
Identity = 66/177 (37.29%), Postives = 89/177 (50.28%), Query Frame = 0
Query: 1302 LLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTH--FIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPEL 1476
            LL  N      +KNLD     ETHKI V YI   Q  D+  IL N  GS  Y DF+ GLG       +D STH  F+GGL      G  A  +    ++ IFHV+T MP+ D+D    KK RH+GN+   IV+++   HS  Y+ G +   F    I+I P+      + +  KPE+
Sbjct: 1623 LLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQ-EDKCSILANERGSQAYEDFVAGLG-----WEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPS-DSDDSLTKKLRHLGNDEVHIVWSE---HSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEV 1789          
BLAST of EMLSAG00000002599 vs. SwissProt
Match: gi|147742932|sp|Q2PPJ7.2|RGPA2_HUMAN (RecName: Full=Ral GTPase-activating protein subunit alpha-2; AltName: Full=250 kDa substrate of Akt; Short=AS250; AltName: Full=p220)

HSP 1 Score: 103.605 bits (257), Expect = 1.090e-20
Identity = 66/177 (37.29%), Postives = 89/177 (50.28%), Query Frame = 0
Query: 1302 LLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTH--FIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPEL 1476
            LL  N      +KNLD     ETHKI V YI   Q  D+  IL N  GS  Y DF+ GLG       +D STH  F+GGL      G  A  +    ++ IFHV+T MP+ D+D    KK RH+GN+   IV+++   HS  Y+ G +   F    I+I P+      + +  KPE+
Sbjct: 1624 LLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQ-EDKCSILSNERGSQAYEDFVAGLG-----WEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPS-DSDDSLTKKLRHLGNDEVHIVWSE---HSRDYRRGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEV 1790          
BLAST of EMLSAG00000002599 vs. SwissProt
Match: gi|147742933|sp|A3KGS3.2|RGPA2_MOUSE (RecName: Full=Ral GTPase-activating protein subunit alpha-2; AltName: Full=250 kDa substrate of Akt; Short=AS250; AltName: Full=P220)

HSP 1 Score: 103.219 bits (256), Expect = 1.227e-20
Identity = 66/177 (37.29%), Postives = 89/177 (50.28%), Query Frame = 0
Query: 1302 LLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTH--FIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPEL 1476
            LL  N      +KNLD     ETHKI V YI   Q  D+  IL N  GS  Y DF+ GLG       +D STH  F+GGL      G  A  +    ++ IFHV+T MP+ D+D    KK RH+GN+   IV+++   HS  Y+ G +   F    I+I P+      + +  KPE+
Sbjct: 1623 LLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQ-EDKCSILANERGSQAYEDFVAGLG-----WEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPS-DSDDSLTKKLRHLGNDEVHIVWSE---HSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEV 1789          
BLAST of EMLSAG00000002599 vs. SwissProt
Match: gi|51315849|sp|Q6GYP7.1|RGPA1_MOUSE (RecName: Full=Ral GTPase-activating protein subunit alpha-1; AltName: Full=GAP-related-interacting partner to E12; Short=GRIPE; AltName: Full=GTPase-activating RapGAP domain-like 1; AltName: Full=Tuberin-like protein 1; AltName: Full=p240)

HSP 1 Score: 102.449 bits (254), Expect = 2.069e-20
Identity = 73/225 (32.44%), Postives = 114/225 (50.67%), Query Frame = 0
Query: 1302 LLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELK--EPL--GHVMS---LSILVRQWALHCNLAAQIQKSLSNKMPYSSNWLZ 1519
            LL  N+     ++NLD     ETHKI V Y+   Q  D+  IL NI GS  Y DF+ GLG  +N+ N      F+GGL      G     +    ++ IFHV+T MP+ D+D    KK RH+GN+   IV+++   H+  Y+ G +  +F    IVI P+      + +  KPE+    PL  G +++   L I+VR  A++ +      ++L + +P   N+ +
Sbjct: 1784 LLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQ-EDKYSILTNIGGSQAYEDFVAGLGWEVNLTN---HCGFMGGLQKNRSTGLTTPYFATSTVEVIFHVSTRMPS-DSDDSLTKKLRHLGNDEVHIVWSE---HTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINAS------RALKSLIPLYQNFYE 1994          
BLAST of EMLSAG00000002599 vs. SwissProt
Match: gi|703556455|sp|O55007.2|RGPA1_RAT (RecName: Full=Ral GTPase-activating protein subunit alpha-1; AltName: Full=GAP-related-interacting partner to E12; Short=GRIPE; AltName: Full=GTPase-activating RapGAP domain-like 1; AltName: Full=Tuberin-like protein 1; AltName: Full=p240)

HSP 1 Score: 100.908 bits (250), Expect = 5.837e-20
Identity = 70/206 (33.98%), Postives = 106/206 (51.46%), Query Frame = 0
Query: 1302 LLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELK--EPL--GHVMS---LSILVRQWALHCNLA 1500
            LL  N+     ++NLD     ETHKI V Y+   Q  D+  IL NI GS  Y DF+ GLG  +N+ N      F+GGL   +  G     +    ++ IFHV+T MP+  +D    KK RH+GN+   IV+++   H+  Y+ G +  +F    IVI P+      + +  KPE+    PL  G +++   L I+VR  A++ + A
Sbjct: 1784 LLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQ-EDKYSILTNIGGSQAYEDFVAGLGWEVNLTN---HCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSESDD-SLTKKLRHLGNDEVHIVWSE---HTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRA 1981          
BLAST of EMLSAG00000002599 vs. SwissProt
Match: gi|51315850|sp|Q6GYQ0.1|RGPA1_HUMAN (RecName: Full=Ral GTPase-activating protein subunit alpha-1; AltName: Full=GAP-related-interacting partner to E12; Short=GRIPE; AltName: Full=GTPase-activating Rap/Ran-GAP domain-like 1; AltName: Full=Tuberin-like protein 1; AltName: Full=p240)

HSP 1 Score: 99.3673 bits (246), Expect = 1.838e-19
Identity = 72/225 (32.00%), Postives = 114/225 (50.67%), Query Frame = 0
Query: 1302 LLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELK--EPL--GHVMS---LSILVRQWALHCNLAAQIQKSLSNKMPYSSNWLZ 1519
            LL  N+     ++NLD     ETHKI V Y+   Q  D+  IL N  GS  Y DF+ GLG  +N+ N      F+GGL   +  G     +    ++ IFHV+T MP+ D+D    KK RH+GN+   IV+++   H+  Y+ G +  +F    IVI P+      + +  KPE+    PL  G +++   L I+VR  A++ +      ++L + +P   N+ +
Sbjct: 1785 LLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQ-EDKHSILTNTGGSQAYEDFVAGLGWEVNLTN---HCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPS-DSDDSLTKKLRHLGNDEVHIVWSE---HTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINAS------RALKSLIPLYQNFYE 1995          
BLAST of EMLSAG00000002599 vs. Select Arthropod Genomes
Match: XP_006572241.1 (PREDICTED: tuberin isoform X1 [Apis mellifera])

HSP 1 Score: 775.778 bits (2002), Expect = 0.000e+0
Identity = 469/1315 (35.67%), Postives = 706/1315 (53.69%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLG-LVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP-QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRS-------------------------------------------------LEQEAERDSLLKDL----------LIVVAEDESYHLGLEESD--GSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAK---KDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKD---------------DITKNSDRRRHMSDFYSGTNKSQLSN-----LRSDKE----LHRHTSLERME-SSGDDYRVKKVLPET-------CSCWCSGWAEIYIRRPSGDVSWMCRVQNT------------QLLSAHTPSTNLRELTALFDTSLKKDTFNFDVSTESDKINVD-NLTEDEYKKLSSE--FVPSSVSSPSAETDKTVSGNRS-HDEEMIVMSPKSPVKKTKSSP 1186
            MS   K  K   +KLKQ+F+  K N Y   E+  L+ +L   ISP  SP+  R K +KDL ++V N + +DK  E L Y  KDLL  D P + RH+  +F   LV GQYS+L  L+R  FF ++KE++      D    ++LL  LT  GKDI++ E+ +GPF+ + +P +T        +FL ++ N   FN+ Y+D D++  L+  I  LC  +N       CLE L++I+CYS +  + L  FI  LC  VN+E Y + +W I R L+GTH+GHSALY +  ++Q T  + DV L+RGA+F++   LWG ++I  L+ +  ++LP+F  ALKC H +V++EVI  +++LV  Y  EL       I+++IE ++       +     +S +LH  I  +E L ++  Y+G  Q+ + +++ C+D RPE SV++LI +R +SI      W    + L+ERYYK E R NIR+K LDV+ ++ + N   +EEELI+RII+P+   V M+ +  IR     +L  LC  C T + L+LL+IL  ++ +                                                  LEQ  +  ++  D+           + +  +  YHLG  ++       FSPY+I     E    E + +    ++  P +P+ L     + +  +SL  AC  VI C++ E DW VL LVL  +P V+QNR LI  R+   I+ F   L S  +DK+   P  L N P KF+ S+F  H+FPVLA+LASY+  L P  Q  LI+ LE+GL +K C   C+ +LTT  LEM+ +   L+ EVLLN SKISAT  I+ PILEFLST+ RLP+VF+ F    Y++VFAI LP+TNPFK+D YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N+ VPFEE        N  N+DS  R RS+SL    S  RR    +  +    +  +D+        + FH ELTETC+D++ARY +   S + +RL ++DF+LK G S TWL+G  LITV+TS C++ AMRNG+CD C+  C + G  S +               +  K     R  S  Y  +  +  +N     L+   E    + +  SLE+++    +  +++++L          C+CWC GWAEIY+RRP+GD+SW+ R+QN+             +++ + P+ +L++    F +    D          D+I  D N  +     +SS    +P S + PS     +     S  D E  +   ++PV+++ SSP
Sbjct:    4 MSSKDKDNKTLHDKLKQFFRINKGN-YKSREDFTLTHDLEKEISPE-SPVHHRTKAIKDLCDAVLNQQWEDKAAERLWYLVKDLLEKDVPREHRHVTFNFLRCLVQGQYSKLSSLMRVKFFMVVKEHNIPE---DIGPRLELLQSLTENGKDILHLEERMGPFLLDWMPIVTAGDGKRGAEFLLLLVNVIKFNSAYIDEDIISGLVQYICHLCYYSNSSEVVSGCLEALDAIVCYSNLQSDSLQTFIIALCGSVNVETYCQISWKIMRNLLGTHMGHSALYTMCRLLQDTNFQRDVRLLRGAVFYVNMGLWGTHKIPKLECTPTSVLPSFYQALKCNHPVVMYEVILSIQRLVNKYGTELWDPTWSIILDIIEEVISHTETSNQPATRQVSVNLHETINSIENLLDINHYNGCIQRFYDLVERCSDARPESSVLKLIEYRARSIGPTHYHWQFKLANLMERYYKIETRTNIRMKVLDVLTNVVQINRSRYEEELIERIIVPYFQHVDMDFDITIRNGVAHLLIDLCLECDTKRCLELLDILEKVINKPFTSDIPITKDVDIKDIKTAVVGVIKILISKIYYLPSSHAIRAYKVLVNYLEQHYKEPTVFYDIPTIRYLIFECFLKIRANTLYHLGFPDTQNLSVIKFSPYLIL----EHTTTERINSGGGGNSPPPVNPAPLQHLSCQ-ITYMSLALACKAVISCIKLEKDWKVLQLVLKELPQVMQNRALILSRHTNDIDYFANALCSMVSDKSLRLPESLYNVPPKFTLSEFRVHVFPVLASLASYHAHLEPNLQQRLIKCLEVGLTAK-CASQCVTSLTTCILEMRDAMNKLLSEVLLNLSKISATVHIAIPILEFLSTLTRLPKVFASFIGDQYMSVFAILLPYTNPFKYDHYTVSLAHHVIAVWFLKCRLPFRRDFVRFITTGLKANVIVPFEEGHLMKSDFNFINEDSSNRKRSSSLTEQGSRGRRERPIMTNRMIGDNKVSDLKPPIDEALMTFHVELTETCIDLMARYTFSTYSARPKRLPSADFLLKEGQSMTWLLGNKLITVTTSGCSNKAMRNGLCDNCWVVC-KSGPQSPEHSKALQSNLRRASSIETAKEDKLSRQFSGGYGNSTVNTAANSPTEELKKTSEDLDSIKKDHSLEKIDKDQAESSKLEQILNSEKQEEHVLCACWCQGWAEIYVRRPTGDMSWIMRIQNSMQFETNIDFPVYDIMALYRPNQDLKQKQQEFTSEYSGDETEDIADKNMDQIKNDHNNMKSMISSVSSGPIAIPGSPARPSPSRQSSRDSLESLEDGEDDLRRSRNPVRRSNSSP 1306          

HSP 2 Score: 275.789 bits (704), Expect = 6.499e-74
Identity = 152/346 (43.93%), Postives = 209/346 (60.40%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGE----KNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNLAAQIQKSLSNKM--PYSSNWLZXLRAIKRLKEKLQKEN-----EMKEEEDREQGSGRPQLNDFTEF 1558
            R HTISV SP  +              R     I  G     K    +GI+P FVFL LY ++ FG+ +  EKPLL+P     + ++ NLDRI  +ETHKIGVLY+GP Q ++E  IL N  GS RYT+FL  LG L+ ++++D+S  F+GGLD    +GNFAY+W DDV Q  FHVATLMP   +DP+   KK+HIGNNY  +VYN+S    ESY + TV+G+F  AC+VI PLD G+N+V V  K EL + + H         +L+IL RQ ALH NLA+ +  SL      PY+SNWL+ LR IKRL+ ++ +E+     +   ++   + + R  ++DFTE+
Sbjct: 1498 RGHTISVMSPVKK-------------SRGEWDNIRRGNSPRIKETPKTGINPSFVFLQLYHTAHFGSPS--EKPLLVPQTTAVQRAVTNLDRIQPYETHKIGVLYVGPGQASNETEILANQHGSLRYTEFLQRLGTLVRLKDVDESV-FLGGLDRNGENGNFAYIWQDDVTQVAFHVATLMPTKLSDPKCTSKKQHIGNNYVTVVYNES---GESYNIQTVKGQFNYACVVIQPLDHGTNQVTVQVKEELAKHIRHSEPKIISDQNLAILSRQLALHANLASMVSSSLEQNSHNPYASNWLERLRHIKRLRNRVLQESTNNNPDGTTDDLSPRSNKRIYMDDFTEY 1824          
BLAST of EMLSAG00000002599 vs. Select Arthropod Genomes
Match: XP_016772912.1 (PREDICTED: tuberin isoform X2 [Apis mellifera])

HSP 1 Score: 775.393 bits (2001), Expect = 0.000e+0
Identity = 469/1315 (35.67%), Postives = 706/1315 (53.69%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLG-LVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP-QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRS-------------------------------------------------LEQEAERDSLLKDL----------LIVVAEDESYHLGLEESD--GSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAK---KDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKD---------------DITKNSDRRRHMSDFYSGTNKSQLSN-----LRSDKE----LHRHTSLERME-SSGDDYRVKKVLPET-------CSCWCSGWAEIYIRRPSGDVSWMCRVQNT------------QLLSAHTPSTNLRELTALFDTSLKKDTFNFDVSTESDKINVD-NLTEDEYKKLSSE--FVPSSVSSPSAETDKTVSGNRS-HDEEMIVMSPKSPVKKTKSSP 1186
            MS   K  K   +KLKQ+F+  K N Y   E+  L+ +L   ISP  SP+  R K +KDL ++V N + +DK  E L Y  KDLL  D P + RH+  +F   LV GQYS+L  L+R  FF ++KE++      D    ++LL  LT  GKDI++ E+ +GPF+ + +P +T        +FL ++ N   FN+ Y+D D++  L+  I  LC  +N       CLE L++I+CYS +  + L  FI  LC  VN+E Y + +W I R L+GTH+GHSALY +  ++Q T  + DV L+RGA+F++   LWG ++I  L+ +  ++LP+F  ALKC H +V++EVI  +++LV  Y  EL       I+++IE ++       +     +S +LH  I  +E L ++  Y+G  Q+ + +++ C+D RPE SV++LI +R +SI      W    + L+ERYYK E R NIR+K LDV+ ++ + N   +EEELI+RII+P+   V M+ +  IR     +L  LC  C T + L+LL+IL  ++ +                                                  LEQ  +  ++  D+           + +  +  YHLG  ++       FSPY+I     E    E + +    ++  P +P+ L     + +  +SL  AC  VI C++ E DW VL LVL  +P V+QNR LI  R+   I+ F   L S  +DK+   P  L N P KF+ S+F  H+FPVLA+LASY+  L P  Q  LI+ LE+GL +K C   C+ +LTT  LEM+ +   L+ EVLLN SKISAT  I+ PILEFLST+ RLP+VF+ F    Y++VFAI LP+TNPFK+D YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N+ VPFEE        N  N+DS  R RS+SL    S  RR    +  +    +  +D+        + FH ELTETC+D++ARY +   S + +RL ++DF+LK G S TWL+G  LITV+TS C++ AMRNG+CD C+  C + G  S +               +  K     R  S  Y  +  +  +N     L+   E    + +  SLE+++    +  +++++L          C+CWC GWAEIY+RRP+GD+SW+ R+QN+             +++ + P+ +L++    F +    D          D+I  D N  +     +SS    +P S + PS     +     S  D E  +   ++PV+++ SSP
Sbjct:    4 MSSKDKDNKTLHDKLKQFFRINKGN-YKSREDFTLTHDLEKEISPE-SPVHHRTKAIKDLCDAVLNQQWEDKAAERLWYLVKDLLEKDVPREHRHVTFNFLRCLVQGQYSKLSSLMRVKFFMVVKEHNIPE---DIGPRLELLQSLTENGKDILHLEERMGPFLLDWMPIVTAGDGKRGAEFLLLLVNVIKFNSAYIDEDIISGLVQYICHLCYYSNSSEVVSGCLEALDAIVCYSNLQSDSLQTFIIALCGSVNVETYCQISWKIMRNLLGTHMGHSALYTMCRLLQDTNFQRDVRLLRGAVFYVNMGLWGTHKIPKLECTPTSVLPSFYQALKCNHPVVMYEVILSIQRLVNKYGTELWDPTWSIILDIIEEVISHTETSNQPATRQVSVNLHETINSIENLLDINHYNGCIQRFYDLVERCSDARPESSVLKLIEYRARSIGPTHYHWQFKLANLMERYYKIETRTNIRMKVLDVLTNVVQINRSRYEEELIERIIVPYFQHVDMDFDITIRNGVAHLLIDLCLECDTKRCLELLDILEKVINKPFTSDIPITKDVDIKDIKTAVVGVIKILISKIYYLPSSHAIRAYKVLVNYLEQHYKEPTVFYDIPTIRYLIFECFLKIRANTLYHLGFPDTQNLSVIKFSPYLIL----EHTTTERINSGGGGNSPPPVNPAPLQHLSCQ-ITYMSLALACKAVISCIKLEKDWKVLQLVLKELPQVMQNRALILSRHTNDIDYFANALCSMVSDKSLRLPESLYNVPPKFTLSEFRVHVFPVLASLASYHAHLEPNLQQRLIKCLEVGLTAK-CASQCVTSLTTCILEMRDAMNKLLSEVLLNLSKISATVHIAIPILEFLSTLTRLPKVFASFIGDQYMSVFAILLPYTNPFKYDHYTVSLAHHVIAVWFLKCRLPFRRDFVRFITTGLKANVIVPFEEGHLMKSDFNFINEDSSNRKRSSSLTEQGSRGRRERPIMTNRMIGDNKVSDLKPPIDEALMTFHVELTETCIDLMARYTFSTYSARPKRLPSADFLLKEGQSMTWLLGNKLITVTTSGCSNKAMRNGLCDNCWVVC-KSGPQSPEHSKALQSNLRRASSIETAKEDKLSRQFSGGYGNSTVNTAANSPTEELKKTSEDLDSIKKDHSLEKIDKDQAESSKLEQILNSEKQEEHVLCACWCQGWAEIYVRRPTGDMSWIMRIQNSMQFETNIDFPVYDIMALYRPNQDLKQKQQEFTSEYSGDETEDIADKNMDQIKNDHNNMKSMISSVSSGPIAIPGSPARPSPSRQSSRDSLESLEDGEDDLRRSRNPVRRSNSSP 1306          

HSP 2 Score: 241.121 bits (614), Expect = 3.343e-63
Identity = 130/280 (46.43%), Postives = 171/280 (61.07%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGE----KNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNL 1499
            R HTISV SP  +              R     I  G     K    +GI+P FVFL LY ++ FG+ +  EKPLL+P     + ++ NLDRI  +ETHKIGVLY+GP Q ++E  IL N  GS RYT+FL  LG L+ ++++D+S  F+GGLD    +GNFAY+W DDV Q  FHVATLMP   +DP+   KK+HIGNNY  +VYN+S    ESY + TV+G+F  AC+VI PLD G+N+V V  K EL + + H         +L+IL RQ ALH N+
Sbjct: 1498 RGHTISVMSPVKK-------------SRGEWDNIRRGNSPRIKETPKTGINPSFVFLQLYHTAHFGSPS--EKPLLVPQTTAVQRAVTNLDRIQPYETHKIGVLYVGPGQASNETEILANQHGSLRYTEFLQRLGTLVRLKDVDESV-FLGGLDRNGENGNFAYIWQDDVTQVAFHVATLMPTKLSDPKCTSKKQHIGNNYVTVVYNES---GESYNIQTVKGQFNYACVVIQPLDHGTNQVTVQVKEELAKHIRHSEPKIISDQNLAILSRQLALHANV 1758          
BLAST of EMLSAG00000002599 vs. Select Arthropod Genomes
Match: XP_006572242.1 (PREDICTED: tuberin isoform X3 [Apis mellifera])

HSP 1 Score: 774.622 bits (1999), Expect = 0.000e+0
Identity = 467/1315 (35.51%), Postives = 706/1315 (53.69%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLG-LVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP-QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRS-------------------------------------------------LEQEAERDSLLKDL----------LIVVAEDESYHLGLEESD--GSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAK---KDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKD---------------DITKNSDRRRHMSDFYSGTNKSQLSNLRSDK---------ELHRHTSLERME-SSGDDYRVKKVLPET-------CSCWCSGWAEIYIRRPSGDVSWMCRVQNT------------QLLSAHTPSTNLRELTALFDTSLKKDTFNFDVSTESDKINVD-NLTEDEYKKLSSE--FVPSSVSSPSAETDKTVSGNRS-HDEEMIVMSPKSPVKKTKSSP 1186
            MS   K  K   +KLKQ+F+  K N Y   E+  L+ +L   ISP  SP+  R K +KDL ++V N + +DK  E L Y  KDLL  D P + RH+  +F   LV GQYS+L  L+R  FF ++KE++      D    ++LL  LT  GKDI++ E+ +GPF+ + +P +T        +FL ++ N   FN+ Y+D D++  L+  I  LC  +N       CLE L++I+CYS +  + L  FI  LC  VN+E Y + +W I R L+GTH+GHSALY +  ++Q T  + DV L+RGA+F++   LWG ++I  L+ +  ++LP+F  ALKC H +V++EVI  +++LV  Y  EL       I+++IE ++       +     +S +LH  I  +E L ++  Y+G  Q+ + +++ C+D RPE SV++LI +R +SI      W    + L+ERYYK E R NIR+K LDV+ ++ + N   +EEELI+RII+P+   V M+ +  IR     +L  LC  C T + L+LL+IL  ++ +                                                  LEQ  +  ++  D+           + +  +  YHLG  ++       FSPY+I     E    E + +    ++  P +P+ L     + +  +SL  AC  VI C++ E DW VL LVL  +P V+QNR LI  R+   I+ F   L S  +DK+   P  L N P KF+ S+F  H+FPVLA+LASY+  L P  Q  LI+ LE+GL +K C   C+ +LTT  LEM+ +   L+ EVLLN SKISAT  I+ PILEFLST+ RLP+VF+ F    Y++VFAI LP+TNPFK+D YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N+ VPFEE        N  N+DS  R RS+SL    S  RR    +  +    +  +D+        + FH ELTETC+D++ARY +   S + +RL ++DF+LK G S TWL+G  LITV+TS C++ AMRNG+CD C+  C + G  S +               +  K     R  S  Y  +  +  +N  +++          + +  SLE+++    +  +++++L          C+CWC GWAEIY+RRP+GD+SW+ R+QN+             +++ + P+ +L++    F +    D          D+I  D N  +     +SS    +P S + PS     +     S  D E  +   ++PV+++ SSP
Sbjct:    4 MSSKDKDNKTLHDKLKQFFRINKGN-YKSREDFTLTHDLEKEISPE-SPVHHRTKAIKDLCDAVLNQQWEDKAAERLWYLVKDLLEKDVPREHRHVTFNFLRCLVQGQYSKLSSLMRVKFFMVVKEHNIPE---DIGPRLELLQSLTENGKDILHLEERMGPFLLDWMPIVTAGDGKRGAEFLLLLVNVIKFNSAYIDEDIISGLVQYICHLCYYSNSSEVVSGCLEALDAIVCYSNLQSDSLQTFIIALCGSVNVETYCQISWKIMRNLLGTHMGHSALYTMCRLLQDTNFQRDVRLLRGAVFYVNMGLWGTHKIPKLECTPTSVLPSFYQALKCNHPVVMYEVILSIQRLVNKYGTELWDPTWSIILDIIEEVISHTETSNQPATRQVSVNLHETINSIENLLDINHYNGCIQRFYDLVERCSDARPESSVLKLIEYRARSIGPTHYHWQFKLANLMERYYKIETRTNIRMKVLDVLTNVVQINRSRYEEELIERIIVPYFQHVDMDFDITIRNGVAHLLIDLCLECDTKRCLELLDILEKVINKPFTSDIPITKDVDIKDIKTAVVGVIKILISKIYYLPSSHAIRAYKVLVNYLEQHYKEPTVFYDIPTIRYLIFECFLKIRANTLYHLGFPDTQNLSVIKFSPYLIL----EHTTTERINSGGGGNSPPPVNPAPLQHLSCQ-ITYMSLALACKAVISCIKLEKDWKVLQLVLKELPQVMQNRALILSRHTNDIDYFANALCSMVSDKSLRLPESLYNVPPKFTLSEFRVHVFPVLASLASYHAHLEPNLQQRLIKCLEVGLTAK-CASQCVTSLTTCILEMRDAMNKLLSEVLLNLSKISATVHIAIPILEFLSTLTRLPKVFASFIGDQYMSVFAILLPYTNPFKYDHYTVSLAHHVIAVWFLKCRLPFRRDFVRFITTGLKANVIVPFEEGHLMKSDFNFINEDSSNRKRSSSLTEQGSRGRRERPIMTNRMIGDNKVSDLKPPIDEALMTFHVELTETCIDLMARYTFSTYSARPKRLPSADFLLKEGQSMTWLLGNKLITVTTSGCSNKAMRNGLCDNCWVVC-KSGPQSPEHSKALQSNLRRASSIETAKEDKLSRQFSGGYGNSTVNTAANSPTEELKKTSEDLDSIKKDHSLEKIDKDQAESSKLEQILNSEKQEEHVLCACWCQGWAEIYVRRPTGDMSWIMRIQNSMQFETNIDFPVYDIMALYRPNQDLKQKQQEFTSEYSGDETEDIADKNMDQIKNDHNNMKSMISSVSSGPIAIPGSPARPSPSRQSSRDSLESLEDGEDDLRRSRNPVRRSNSSP 1306          
BLAST of EMLSAG00000002599 vs. Select Arthropod Genomes
Match: EEB16407.1 (tuberin, putative [Pediculus humanus corporis])

HSP 1 Score: 710.294 bits (1832), Expect = 0.000e+0
Identity = 421/1213 (34.71%), Postives = 667/1213 (54.99%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNL--YPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRND-SPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIR----------------RRAIEVLTTLCSL------------------CQTNKNLD------------------------------LLNILGCIMKRSLEQEAERD--SLLKDLLIVVAEDESYHLGLEESDGSXXFSPYIICRSP-EEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPL-ICRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSLS--AARRPNVALAAKKDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRD--------GTLSKDDITKNSDRRRHMSDFYSGTNKSQLSN--LRSDKEL-----------HRHTSLERMESSGDDYRVKKVLPETCSCWCSGWAEIYIRRPSGDVSWMCRVQNT---QLLSAHTPSTNLRELTALF 1107
            MS   K  KP  EKLKQ+F+  KS+   Y    +  ++ E+  SIS   + + +R++ +K+L E V   RL+D  VE L    +DLL+ +   + R   L F + LV GQY +LG++R+ FFNIIKE+     + D +Y +DLL  LT  G+DI++ EDE   F+ +  P +   +    + L +  N   +NA+Y+D + +  ++ L   LC+ +N      + L+VL++I+CYS +P + L +FIS LCR VN+  Y  + W I R L+GTH+GHS LY +  I+Q     +DV L+RG +F+I   LW + R+ SL  +  ++LP+ + A+ C H +V++E+   + +LV  +  EL   A D ++ ++E ++  V   +  L  ++ + LH  +  +E L E+ +++G+ +++F ++++ +  RPE SVIRLI F   SI   + +WL     L+E+Y+K++ R N+R+KTL+V+  +  SN   +E+ELI+RI+IP+L ++  +P+  +R                ++ +E+LT L  L                  C+ N  L+                              L+  L    K S  Q+  ++   ++ + L+ +  ++  HLGL ++  S  +S Y+I  S    ++      +    S +  + PSC+       V   S   A  ++I  L+ E DW VLCL+L  +P++LQ++ L + ++G  I      L S  +DK  N P  L N P K ++++F +++FPV+AA++SY+  L P+ Q   I+ LE GL S+ C   C++ALTT  LEM+ +   L+PEVLLN SKISAT  I+ PILEFLST+  LP+VF+ F    Y++VFAIALP+TNPFK++ YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N   PFEE        +  N+DS  R RS+SL+   +RR    ++    + AD+          FH ELTETC+D++ARY + + S + +R   ++F+L  G S TWL+G  LITV+TS C+   ++N +CDRC+  C  D          + KDD+ K S +     +     ++SQ  N  + S  E            H  + LE+     +D   +  L   C+CWC GWAEIY++RP+GD+SW+ R+QN    Q LS   P   L ++T LF
Sbjct:    1 MSGRDKEIKPL-EKLKQFFRAAKSSSVNYKGKADFVITYEIEKSISAD-TQIHQRIRTIKELSEIVATCRLEDHAVEKLWTLVRDLLQKEVEKEHRQACLHFLKCLVQGQYEKLGIMRTHFFNIIKEHDL---TDDVSYLLDLLQALTDNGRDILHLEDEFSEFLLKWTPKVI-GVGKAAEVLSLFLNVIKYNASYIDDNNMADIVDLACFLCSWSNKDEVISTSLQVLDTIVCYSNLPPDSLSVFISALCRKVNVHEYSHNCWKIMRNLLGTHMGHSTLYTMCKILQDNAYYNDVGLLRGTVFYINMGLWSNKRVTSLRCTLTSVLPSLLQAMHCNHTVVIYEITISIHRLVNKFGLELQDPAWDVVLSILEAIIEHVETKDVSLLGSVITKLHETLCTIEQLIELGQFNGNVRRVFSLIEMSSCMRPESSVIRLINFLSGSIVPTRYEWLTKLDGLLEKYFKQDTRPNVRVKTLEVLSLVVNSNKLIYEDELIERIVIPYLQNLDQDPDINVRNVGAQILVNLCIGCESKKCLELLTVLEKLLNRKEQHSFENIQKEGNGCE-NDGLEIKSVVSGLIKIFTLKMYHLPSNHALKAYKILIGFLESCYKNSYHQDYNQNIKFMIFECLLKLRVNKIGHLGLLDATNSVRYSSYLIIESKLNSDKGGPGTISPPPISPAPSNYPSCV-------VTPFSFATASKVIISALKNEKDWKVLCLLLQELPSILQSKSLMLSKHGTDIGLLADALCSMVSDKTLNLPESLRNIPQKLNRTEFQSYVFPVIAAISSYHLNLEPSQQQRCIKCLEYGLASR-CASQCVMALTTCTLEMRDAMYKLLPEVLLNLSKISATVVIAIPILEFLSTLTSLPKVFASFVGDQYMSVFAIALPYTNPFKYNHYTVSLAHHVIAVWFLKCRLPFRRDFVRFITTGLKANAIAPFEEEKLLKCDLSSLNEDSTSRKRSSSLTEQGSRRRERPISTAPRIGADLKPPLDEALFNFHVELTETCIDLMARYTFSSCSARPKRSPIAEFLLSGGQSQTWLLGHKLITVTTSGCSQKPLKNNLCDRCWMVCKSDKKFMSPDGDEIEKDDLLKLSRQNSGEKNLDQNISESQKFNRDMSSGGESPTTDDNKKCANHETSKLEKFIFGNNDNTSRNDL---CACWCQGWAEIYVKRPTGDMSWVLRIQNETNYQNLSQELP---LPDVTTLF 1192          

HSP 2 Score: 279.641 bits (714), Expect = 4.347e-75
Identity = 181/472 (38.35%), Postives = 251/472 (53.18%), Query Frame = 0
Query: 1137 YKKLSSEFVPSSVSSPSAETDKTVSGNRSHDEEMIVMSPKSPVKK-TKSSPPDTSCQPLEVSDIKRKK---------NQNNIPLNS----SPNSSF---------ESN--LNEPAMR--------TTRSHTISVSSP--RNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNLAAQIQKSL--SNKMPYSSNWLZXLRAIKRLKEKLQKENEMKEEEDREQGSGRPQ------LNDFTEF 1558
            Y K+S E +P  +S        +V     H    I +S K P    ++ SP      P     IKR           ++ N+ ++S    +P+ S+         ESN  LN    R          RS TISV SP  ++R  N  L+P                 K+   SG+SP FVFL LY S+ FG+    EKP+L+  N + + +I  LD IP  ETHK+GV+Y+GP Q N E  ILRN  GS RY  FL  LG LIN++N D  + F+GGL     DG FAY+W DDV Q  FHVATLMPN ++DP  + KK HIGN++  IVYN+S    E Y + TV+G+F  A ++I PLD  +N+V V  + EL + +GH         +L+IL RQ ALH NLA+ + +SL   N+ PY+SNWL+ LR IKRL++K+ +    +E  D ++    P+      ++DFTE+
Sbjct: 1330 YGKVSCEAIPEEMSGMGTTPPDSV-----HQPLTIAISSKPPPHSFSQQSPTQKKTYPKMGGGIKRDSPKFDGKSFADKQNLNIDSLGDKNPSKSYSSSGEIGKCESNRSLNSDENRLDLSQTYKRDRSRTISVMSPARKSRYDNDHLKPKLS----------SPRTKDAPRSGVSPSFVFLQLYHSAHFGSTE--EKPILISQNSVIQRAILTLDFIPPFETHKVGVIYVGPGQANCEAEILRNEFGSLRYAIFLQSLGTLINLKNADPKSTFLGGLGKSGVDGKFAYIWQDDVTQVTFHVATLMPNKESDPNCHAKKAHIGNDFVTIVYNES---GEEYNIQTVKGQFNYANVIIQPLDHATNKVVVKVREELADQIGHSEQKIVSDQNLAILARQLALHANLASLVSRSLKAKNQDPYASNWLERLRKIKRLRQKVLQLEISEENADHKEFLNPPKSRKTDYMDDFTEY 1781          
BLAST of EMLSAG00000002599 vs. Select Arthropod Genomes
Match: EFX68537.1 (putative tuberous sclerosis 2 isoform 3-like protein [Daphnia pulex])

HSP 1 Score: 610.527 bits (1573), Expect = 0.000e+0
Identity = 406/1077 (37.70%), Postives = 560/1077 (52.00%), Query Frame = 0
Query:  558 DLLIVVAEDESYHLGLEESDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEEN----DKNKDSMG---------RMRSTSLSAARRPNVALAAKKDVAADVPKXTQIHP-LAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKDDITKNSD------------RRRHMSDFYSGTNKSQLSNLRSDKELHRHTSLERMESSGDDYRV---------KKVLPET--------CSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNLRELTALFDTSLKKDTFNFDVSTESDKINVDNLTEDEYKKL-----SSEFVPSSVSSPSAETDKTVSGNRSHDEEMIVMSPKSPVKKTKSSPPDTSCQPLEVSDIKRKKNQNNIPLNSSPNSSFESNLNEPAMRTTRSHTISVSS----PRNRV-----LNKPLQPTTGIVGRPGIGGIGSGEKN-DKVSGISPQFVFLMLYQSSTFGNAASLEK--------PLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHVM--------SLSILVRQWALHCNLAAQIQKSLSNKMPYSSNWLZXLRAIKRLKEKLQKENEMKEEEDREQGSGRPQLNDFTEF 1558
            D ++ +  D  Y LG         +SPYI+      E          + +   P+S      T    V  +SL +AC+ VI CL +E DW VL LVL R+P VL N+ L+  R+   I+     L S  +D   N P  L N P KFS+++F  ++F VL +LASY+ +L P +Q  L++ LE+GL  K+  + C+VALT SALEMK +   L+P VLL+ SKISAT  I+  ILEFLST+ RLPEV+S F +  Y+++FAI LPF NPFKF+ Y VSLAHHV+ +WFL C+L  R+NFV +I+    +N+ +P EE      +N+ + G         R+RS S S  R  NV    +   A D  K TQ    + FH+EL ETC D+++RY YGN +   ++  T   ILK+G S TWLIG  L+T++TS C  +A + G+CDRC   C      +    T+  D            RRRH S             + S  E  +     R +SS DD R+         ++  PE+        C C+CSGWA+I+IRRP+GD SW+ R+ N        P  ++ ++  L D S +                V NL      KL     +S F P    S SA        NR   +E+ V    S  + ++  PP           I R+ +   IP  SS   S E+ + E +       T  ++     PR+RV     LN   + T     R  +  I S  K+ +  SG +P F+FL LY S       +  K        P+L+P+N     +IKNLDRIP +ETHKIGVLY+G  Q   E  IL N  GS RY +FL  LG LI++  +D    +IGGL+    DG FAY+W DDV+Q IFHVATLMP    DP    KKRHIGNNY  IVYNDS    E ++M T+RG+F  AC+++ PLD  ++RV + C+ +L E +G  +        ++S+L RQ A H NLAA I +S SN  PY+SNWL+ LR IKR K +L+KE+  + E++           DFTEF
Sbjct:  619 DFILNITADGDYRLGYAPLP---RYSPYILIDHKHGERVGGTNRKEIRSATEDPTS------TVHSQVTQMSLGEACVAVIRCLNQERDWRVLKLVLERIPRVLNNKALVLSRHNTDIDYVGRALCSLISDTTLNNPETLRNAPPKFSRAEFQLYVFRVLTSLASYHAYLKPETQQKLVKCLEVGLKFKDTAKTCVVALTISALEMKETMYKLLPGVLLSLSKISATVSIAPNILEFLSTLNRLPEVYSSFVEEQYLSIFAITLPFINPFKFNHYVVSLAHHVVSLWFLSCKLPLRKNFVCYIVKGFKNNVLIPCEEGLSQMAQNQSASGQSSAEELSSRVRSGSFS--RTGNV----RSTGAGDATKVTQDETRIMFHRELMETCADLMSRYTYGNSAPYAQQSDTVGKILKDGPSQTWLIGHKLVTITTSPCLRVANQRGLCDRCSLLCKLGSGETSVPATETDDPPSPVVSDVIGFRRRHRSAMSPAALSPGEHGIDSRTEDIQAKLRSRHKSSIDDRRLTIDMDDPNREQQDPESKASIAQIPCVCYCSGWAKIHIRRPTGDTSWVTRLLN--------PPQSINDVFLLSDWSEEP---------------VPNLATVLIPKLDTLKEASNFHPKLDRSVSASAALRSPSNR---DEIEVGDGISIEEDSRERPP-----------IARQTSLKEIP--SSSRKSCEA-IPEESKEVEGVKTDDITDFHPHPRDRVYTMPALNMQRRGTNDPNLRRNVPNIDSSLKDANASSGSNPSFIFLQLYYSPMTSAVTAANKEGSEAGLPPILVPNN--IHTAIKNLDRIPPYETHKIGVLYVGKGQAGKESEILANEHGSVRYMEFLHKLGQLISLNEVDTRGTYIGGLEVSGADGKFAYLWKDDVLQVIFHVATLMPTLPKDPAGTNKKRHIGNNYVTIVYNDS---GEDHQMNTIRGQFNHACVIVEPLDHATHRVTLKCRDDLHELMGGPVEPKLVSDRNVSLLSRQMAFHSNLAALILQSKSN-YPYASNWLERLRQIKRTKARLEKEDVKEREKNPWLQYNLSSTLDFTEF 1634          

HSP 2 Score: 248.44 bits (633), Expect = 1.854e-65
Identity = 169/554 (30.51%), Postives = 298/554 (53.79%), Query Frame = 0
Query:    4 SSKAEKPFSEKLKQWFKGTKSNL----------YPH--GEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALK-CKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKR 544
            S+  +K F EKLKQ+FK +  +           +P    E+  L+ ++  S++  I P+ +RLK L+++ E V + R++D G E L     DL    S + R   L F ++L+ GQY RLG++R   F+I+KE  S+    +F   I+LL  +T  GKD++Y E+ +G F+ + +  ++       + L  + N   FN+ Y+D +++  +++ +  L  S++D  E    + V ++IICYS +P   +++F S LCR+VN+ +Y   +W I R L+GTHLG S LY L   +QT+E   D +L+RG IFFIG  LW              +LP    ALK  K+ LV+FE    +++LV      L   A D I+E+++  L       K  Q  +      +++ ++ L E   Y GS +  F  ++    +  E SV+ L+ F+ + I  ++  W++  ++ ++R+Y +EKR+  R + LD+I     +     +EEL++ +++P   ++H+E +  +R +A++++ +LC  C +     LL++L  IM+R
Sbjct:    2 SNNRDKTFPEKLKQFFKFSSKSPASSQSQTCGSFPRLKVEKFVLTRDVQRSLTCEI-PVGQRLKTLQEIGEEVASKRIEDDGAETLWLLINDLFVISSTEERQTALMFLKNLITGQYERLGMLRVYIFDIVKE--SQFNEDNFIL-IELLKAMTDNGKDLLYLEESIGGFLLQFLLQVSQLQ-LLKQLLPFLLNVIKFNSAYVDGNMIGGVVSTLCGLAISSSDPEEILLIISVFDAIICYSNLPNESIVVFTSTLCRLVNVSSYSNLSWKIMRNLIGTHLGTSTLYTLIHSLQTSE---DPSLLRGGIFFIGMVLWSDRPANCPICPPAVVLPALSEALKHSKNPLVIFETGLTMQKLVRLDGANLVGLAWDWILEILDHFLQFGEEYPKVRQVFLE-----LLSDVQDLVERGVYMGSVELFFATMERGLPYLNESSVVLLMNFQLEKIKPSQPNWISNLNKYIQRFYVEEKRIQPRFRILDMIESTVVTQRRLHDEELLELVLLPLFQNLHLEHDVSVREKAVDLIVSLCHHCNSKHCTGLLDVLERIMER 542          
BLAST of EMLSAG00000002599 vs. Select Arthropod Genomes
Match: gb|EFA09042.2| (Tuberin-like Protein [Tribolium castaneum])

HSP 1 Score: 512.301 bits (1318), Expect = 5.159e-153
Identity = 349/1194 (29.23%), Postives = 594/1194 (49.75%), Query Frame = 0
Query:    8 EKPFSEKLKQWFKGTKSNLY--PHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISS-HLHAVITILEGLSEMKRYSGSKQKLFGMLDLCA-----------------DHRPEES-----VIRLIT--FRQKSIHAAKSKWLNIFSELVERY--YKKE----------------KRVNIRIKTLDVILDIFKSNHYSF--------------------EEELID-RIIIP-----FLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQEAERDSLLKDLLIVVAEDESYHLGLEESD-GSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKS--INKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGL-LSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRS-----------TSLSAARRPNVALAAKKDVAADVPKXTQIHP-----LAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKDDITKNSDRRRHMSDFYSGTNKSQ--LSNLRSDKELHRHTSLERMESSGDDYRVKKVLPETCSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNLRELTALF 1107
            +K F E+LK  FK  K +L   P   E  L+ EL+  +    +P   R+K +K+L   +   +L+  GVE L    +D+   + P DVRH    F +S++  Q+ R+ ++R+ FF  IK + + +   D  + +DLL  LT  GK++ +FE+E+GPF+   I  ++ ++    +FL+++ N   FN+ +LD   +   +  I  LC +TN       CL++L +++ YS +P   L  F+  LCR VN++ Y   +W   + L+GTHLGHS L+ +   +Q    + D  L+RG +F+I  +LW  N +++L     ++LP+ + A++C H  V +EVI  +++L+     +L     D I+E+I  ++ +V     ++ ++ ++  LH ++  +E L E+  Y GS  + F ++  C+                 + RP ES     +  L++  F+ +     + K LN+  + +  Y  Y+ E                  V +R +   +++D+ K+ + +                     E + +D  +++P     F   +H  P S     A+ +     +  + N           ++K   ++  +   ++ D L+ +  D    LGL + D     +SPY+ C S +  E           + S   +P C        V  +++  A  L++  LE E DW +L LV+ ++P +L N+ ++     +  +N  +  L +     S   L+L+    K ++ +F   +  V+ AL+SY   L    Q  LI  L         C+R  I +LT   ++M+ +   L+P+VLL  SKISATK+I+ P+LEFLST+ +LP V++ F    Y+ VFAIALP+TNPFK+D Y VSLA+HVI +WFLKCRL +R++FV+FI++ L +N+ VPF+E  + K S+  M             T   + RR       K D     P   +  P     L F++EL ETC+D++ARY Y   S    RL T++F+L  G S +W++G  LIT++TS CT   +++GVCD+C+  C           + N+ +    +   SG  + +  L+   S +E  R+ S    +  G D R + ++   C+CWC GWAE+ IRRP+G+VSW+ +VQN      H     L E++ L+
Sbjct:    4 DKTFQERLKNIFK-KKPDLVNVPPNREFILTDELLRDLGKD-APTHTRVKAMKELAPHL--AKLEPNGVEKLWSCIQDMFLREYPNDVRHATFGFLQSVIRSQFERIDVMRAQFFRFIKHHDNPQ---DVAHRVDLLAALTNDGKEVEHFEEEIGPFLLNWITDVS-RVGKLHEFLKILDNVIKFNSPHLDEYTMTGYVKHICVLCCNTNQFPIVILCLQILGTVVAYSILPPESLSQFVGTLCRTVNIKCYCPASWKTMKNLLGTHLGHSTLFTMCRFLQEPNLKHDAGLMRGCVFYIHMALWDINSMQNLCCPPSSVLPSVLQAVRCNHPSVAYEVILGIQKLINKQGLDLLEPTWDIILEIILHIVKQVELAPNEVPNSSTALTLHEILNTIENLIEVGNYKGSMNQFFAVIHECSRDRPETSVLKLIKYQSKNIRPTESAWLQNLFNLLSNYFKPEVRTNIRLKVLNVLEDTIRLYGLYEDELINHIVIPHMQNIAQDDNVVVRSEVAKLLVDLCKNYNSNICTELLEILEKLLQRPPNANNESDFLDVGVLVPGLVQVFARKIHKLPSSH----AVLIYKIFVNFLERN-----------LLKPPFKEFNQSRLMIFDCLLNMRADSYCRLGLPDPDTKQMKYSPYL-CVSYKPNEPIV----TSPPTTSPSQTPVC-------KVTFLAIESAFKLLLNYLENEKDWEILKLVMEKLPKMLGNKAIVLTKSGNPDLNNLVDVLCTMLQNRS---LNLSEV--KVTRLEFQTAVVSVMVALSSYGNSLDQYHQQLLINTLTRSTGTDPRCSRQSINSLTICMVDMRETMMKLLPDVLLRLSKISATKYIAMPVLEFLSTLSQLPNVYANFVGDQYMAVFAIALPYTNPFKYDNYIVSLAYHVISVWFLKCRLPFRRDFVKFIVSGLQTNVMVPFQELSQFKLSLSSMNQDSSDRKRSSSLTEQGSRRR------VKPDPVKKSPFIQKTTPRDKTVLTFYEELNETCIDLMARYTYSPCSALPSRLPTAEFLLNGGISKSWMLGNKLITITTSGCTERVLKHGVCDKCWSLC----------SSHNNSKIARYARSNSGNEEKESFLTRQGSSEEGKRYDSANLPKLIGGDDRREALV---CACWCQGWAEVLIRRPTGNVSWVMKVQNQVSFKQHIFEFPLNEMSTLY 1138          

HSP 2 Score: 229.18 bits (583), Expect = 1.661e-59
Identity = 122/294 (41.50%), Postives = 176/294 (59.86%), Query Frame = 0
Query: 1274 SGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNLAAQIQKSLSNKM--PYSSNWLZXLRAIKRLKEKLQKENEMKEEEDREQGSGRPQLNDFTEF 1558
            SG++P FVFL LY ++ FG+ +  E PL +PS  + + +I  LDR+  +E HKIGVLYI   QV  E  ILRN  G  RY +FL  LG LI + +++    ++GG+D    DG + Y   DD++   FHVATLMPN D+DP  N KK H+GN+   IVYN+S    + + + T R +   A I++ PLD   N+V V  K +L + +G          S+SILVRQ A+H +LAA +  SL +    PY+SNWL+ LR +K L+EK+ +EN  ++E  +     +  + DFT+F
Sbjct: 1419 SGLNPSFVFLQLYHTACFGSTS--EVPLYVPSCTVIDRAIGILDRMYPYEVHKIGVLYINEGQVEKEAEILRNTCGCVRYMEFLQNLGTLIRLADVNPRDIYLGGVDQGGADGKYMYTHQDDLVMLAFHVATLMPNKDSDPNGNYKKAHVGNDNVVIVYNNS---GQEFDISTFRSQVTLAYIIVEPLDYQCNKVEVKVKSDLLKIIGSNEVIVVSDQSVSILVRQLAIHADLAATVLNSLRSHATDPYASNWLERLRKLKNLREKVVQENNKRKEAQKGFSEEKDIVEDFTDF 1707          
BLAST of EMLSAG00000002599 vs. Select Arthropod Genomes
Match: EAA05274.5 (AGAP011123-PA, partial [Anopheles gambiae str. PEST])

HSP 1 Score: 507.679 bits (1306), Expect = 8.071e-150
Identity = 333/1061 (31.39%), Postives = 548/1061 (51.65%), Query Frame = 0
Query:   14 KLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLL-RNDSPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTL--KFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLL--IEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSL--------EQEAERDSLLKDLLI----------------------------------VVAE-----------------DESYHLGL-EESDGS-XXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNK--ACLLVIECLERETDWNVLC-----------LVLTRVPTVLQNRPLICRYGKSINKFITPLVS-FTDKNSNFPLHLT-NTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEV-FSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEENDKNKDSMGRMRSTSLSAARRPNVALAAKKDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYC 992
            ++KQ+F+ +K  +     ++ +S EL   + P  +P+ +R K L+D  + V   RL+   V+ L   TKDLL  N   + R   L+FY  L+ GQY RLGL+R+ F+ +I E+HS+    D +Y +++L +LT  GK+I +FE E+G F+   + +I P     L    LE+I N   +NA  L+   ++ +++ I  +  +  + N    CL VL+  ICY+ IP   L LFI ILCR+VN EAY + +W I + L GT LGH+ L  +  I+       D AL+RGAIF     LWG + +  L  S   +L +F +ALK +H +V +EVI  + +L+     EL+    D I +++  +   + + RL       + +H H  + ++E L +    +   +K++G+++  +  RPE SV +LI +R   I A +S+WL    + ++R+Y+  K  N+RI  +  +  I  +N  ++E+E+++RI+I  LG+V +E +  +R+     L      C+T + ++LL I+  +++R          EQEAE   LL D LI                                  V+A                  + S+H+G  +  DG+   FS Y+    P + +  +     Q   +S+ +S   L+G         ++++  A  L    ++R     VLC           LVLT +P+V+QN+ LI   G  ++     L   +TDK     L ++ N   K   SD+   I P L++LA Y++ L   +Q ++I AL+ GL+S+  +  CI  LT   LEM       + ++LL  SK++ TK ++ P+LEFLST++ +P   F  FT   Y+ V A++LPFTNPF++D YTVSLAH+V+  WF+KC+L  R   V+FI+  L S + VPF++  ++   +              N   + +K    + P    ++   FH EL +TC+D + R+ +   S  ++RL +++F+L  G S TWL+G  LIT++TS C+ +  RNG+CDRC+ YC
Sbjct:   12 RIKQFFRMSKPGMSSSRHDLPISPELERELRPE-TPVTQRCKALRDFGDQVLTSRLEPDAVQSLWELTKDLLVGNKLMEQRQCALAFYCRLIQGQYDRLGLMRAQFYRVI-ESHSE--PEDISYRLEMLKLLTETGKNIQHFEKEIGAFL---LDWIKPIHEAGLIDSLLELIVNLIKYNAASLEPKFLVGVVSYIFDMTCNKQETNTILLCLSVLDCFICYAIIPNESLTLFIIILCRMVNQEAYCQHSWKIMKNLFGTALGHATLLTMCNILNNPAFHQDDALLRGAIFHTNVGLWGISVVPILHCSPSLVLTSFWNALKSRHVIVTYEVILSMNRLIQKSGNELNEPTWDIICDIMTEISHNLAIHRLPA--DHTVVTHFHETLNMVEQLLQQGTLNADPEKVYGLIEHVSAERPEASVRQLIDYRASKISATRSEWLKQLCQFMDRFYRM-KNSNVRIYAVQALERIMTANRAAYEDEILERIVIVHLGTVPLEKDPEVRKAVARTLIEFTIHCETKRCIELLEIVEKLLQRPYEENQVVRSEQEAEDIILLVDGLIRLFIVKLYRLPSLHAIRVYSMLIGLLEQHYQRPHVLANVVTIRYRIFAWMMKARANGSFHIGYPDPEDGNRVRFSHYLGLEGPYQHQSLQ----PQPYLSSQSTSQLNLTGAGGSEAGSKAMDRIPATNLTTISIKRGCQVIVLCLKEEKDWEVIQLVLTELPSVMQNKALI--QGNDVDSLARTLYRMYTDKMQLEKLQVSANVAPKM--SDYMELILPALSSLAMYHQHLDTNTQKNIIDALKQGLISRRPHE-CIRMLTILLLEMPEQLMPKMADLLLELSKMTGTKMVALPVLEFLSTLILVPSFRFGNFTMRTYMCVMAMSLPFTNPFRYDHYTVSLAHYVVAAWFIKCKLPMRHQLVKFIVQGLESYVHVPFQDGSRSFSQV--------------NEDSSERKQGILNSPLNDDMY--RFHCELADTCVDFMVRHTFSPCSGISKRLPSAEFLLSGGQSMTWLVGHDLITITTSGCSGVTFRNGLCDRCHQYC 1037          

HSP 2 Score: 219.164 bits (557), Expect = 2.678e-56
Identity = 117/266 (43.98%), Postives = 162/266 (60.90%), Query Frame = 0
Query: 1276 ISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHVM---------SLSILVRQWALHCNLAAQIQKSL--SNKMPYSSNWLZXLRAIKRLKEK 1530
            +SP FVFL LY    FG+    E PLLL  +K  E +I  LD IP  E HKIGVLY+GP Q  +E  IL+N  GS RY +FL  LG L+ I++  +   FI  L+    DG F Y++ DD++Q  FHVATLMPN   DP  N+KK+HIGN++  IVYN+S    E Y + T+RG++  AC+++ P++  SNRV +  K ++ +   HV          +  +L RQ ALH NLA+ + +SL   N  PY+SNWL+ LR IK ++ +
Sbjct: 1752 VSPSFVFLQLYHHGQFGS----ECPLLL--DKDPEKAIALLDLIPPFEMHKIGVLYVGPGQAGNESEILKNRYGSLRYAEFLSSLGTLVAIKDAKEKNIFID-LESNGKDGAFTYIYQDDIVQLTFHVATLMPNKRQDPHCNEKKKHIGNDFVTIVYNES---GEEYDLKTIRGQYNYACVIVEPIELNSNRVFIRSKDDIAQ---HVTRETKIVSDHTAPLLARQMALHANLASLVAQSLKTKNSSPYASNWLERLRKIKNIRSR 2004          
BLAST of EMLSAG00000002599 vs. Select Arthropod Genomes
Match: gb|KFM71943.1| (Tuberin, partial [Stegodyphus mimosarum])

HSP 1 Score: 348.591 bits (893), Expect = 9.486e-103
Identity = 255/757 (33.69%), Postives = 370/757 (48.88%), Query Frame = 0
Query:  917 LAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYC-------------------------------------------------IRDGTLSKDDITKN----SDRRRHMSDFYSG--TNKSQLSNLRSDKELHRHTSLE-RMESSGDDYRVKKVLPETCSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNLRELTALFDTSLKKDTFNFDV---STESDKINVDNLTEDEYKKLSS-----EFVP---------SSVSSPSAET-----------------DKTVSGNRSHDEEMIVMSPKS-PVKKTKSSPPDTSCQPLEVSDIKRKK-------NQNNIPLNSSPNSSFESNLNEPAMRTT-----RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCK-PELKEPLGHV-------MSLSILVRQWALHCNLAAQI--QKSLSNKMPYSSNWLZXLRAIKRLKEKLQKENEMKEEE-DREQGSGRPQLNDFTEFV 1559
            +A H EL ET LD++AR+ +G  S   +R   S+F+L+ G  ++WL+G  +IT++TS C   + ++G+C++CY  C                                                  R  TLS++ +T       D   H   F S    N+ Q S L SD+    H  ++   E+ G + +   +    CSCWCS WAE++IRRP+G+ SWM ++QN   L +  P   L  LT+L      +D    D    STE D +      E E K++S      E  P          + SSP   T                 + ++  N+   E + + S    P KK +      S +P    D+ R K       N  +  LNS+P     +N  EP    +     R HTISV SP      +PL     ++ +P +         ++ SG+SP FVFL L+  S FG  A   KPLLL   +    ++  LD  P ++THKIGV+Y+GP QV+ EV IL N+ GS+RY +FL GLG+++ + ++D  T ++GGLD +  DG FA +W DDVMQ +FHVATLM N ++D   N+KK+HI N+   IVYN+S    E YK+ T++G   +AC+VI P D  SN V V  K  EL++  GH         SL + VRQ ALH NLA+ I  ++  S K  + SNWL+ LR IKRL+ K+ +E + K E    E  S     +DFT++V
Sbjct:    7 IALHTELMETGLDLMARFTFGMSSNVPKRSPVSEFLLEKGQKSSWLVGNKIITITTSGCGTKSFKHGLCEQCYSSCHDKCKSIESDENDSIFLEHPVRESQQEISSKRESENSFFERKRHRSAIQRRSATLSREMLTSTKQTKDDMLLHCQRFESSDPNNRFQTSYLNSDENQFSHFQVDWTSENKGREDKSNHL----CSCWCSSWAEVHIRRPTGNTSWMMKMQNHLFLPSAPPDIPLSVLTSLVLPQKIEDQDADDYFLSSTEDDVL----AAESELKEVSCDRGGIEISPCPKSNPPMRRTNSSPDMNTSWSNFQPLMQEERKESFESSMESNQDQLENLTIGSTMEFPSKKDERDV--QSSKPSLKLDLSRTKTCKLKSLNFRSDSLNSAPIEKLLNNNTEPQNVVSGSFRDRGHTISVMSPI-----RPLGSEKSVM-KPDV---------NRRSGMSPSFVFLQLFHDSYFGPPA--HKPLLLLPTETNNRALNVLDHTPPYDTHKIGVVYVGPGQVDKEVAILSNVYGSTRYVNFLHGLGSMVRLTDVDPHTTYLGGLDTKGEDGKFALIWHDDVMQVVFHVATLMLNKESDKMRNEKKKHIANDNVIIVYNES---GEDYKLSTIKGHVTNACVVIHPEDFNSNVVLVKLKTKELQDMFGHTESYIVSDESLPVFVRQLALHANLASLIMSRRQHSAKYSHVSNWLERLRTIKRLRAKVVEELKSKAENLGFENFSDSSNFDDFTDYV 733          
BLAST of EMLSAG00000002599 vs. Select Arthropod Genomes
Match: AAF49066.1 (gigas, isoform A [Drosophila melanogaster])

HSP 1 Score: 251.136 bits (640), Expect = 3.606e-66
Identity = 152/512 (29.69%), Postives = 272/512 (53.12%), Query Frame = 0
Query:   49 PLQERLKILKDLKES-VRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHN---RIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLE-----KKLQDNISSHLHAVITILEGLSEMKRYS--GSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQ 548
            P+ +R ++LK+L ++ + N  L +  + +L   T DL+  + P + R I LSFY+ L++ QY  L ++R  FF +I +NH  R   D  + ++LL  LT  GKDI  FE+++G F+   IP IT +      +L+++ N   FNA +LD D+++ ++     L  S         CL +L  +I Y+  P   L   I+ LCR VN   Y   ++ I + L+GT LG+ ++  +  I+       D  L+RGA+F +  +++G N   ++  + Y A  +L  F+ AL  +  +V FEVI  V+ ++T   ++L     D I +++  ++  +   E     K    ++  + H  I  +E L +  R    G+ ++++ +++  AD RPE SV+ LI +R + + A +  WL + ++ V RYY+    VN+RIKT++ ++ I   N   +EEE++ R+++  L  +H+E   ++R      L+   + C T + +DLL+IL  ++ R  E 
Sbjct:   39 PVAQRCRMLKELGDTQLHNFNLDESAITILFNLTNDLIVPNKPAETRQIALSFYKRLIHTQYKNLTIMREKFFLVI-QNHEAR--EDLRHLLELLDTLTDNGKDITNFEEKIGKFMLLWIPAIT-EANLLTPYLDILVNLIKFNAAHLDKDILVGIVQNACDLSCSVTVNEIGLQCLTILEMVIGYTIFPSEPLPQCITTLCRTVNHAPYCPSSFKIMKNLLGTQLGYHSMKMMCSILNDRALYDDAHLLRGAVFHLNMNIFGSNIIFQVSPMTY-ATNVLTAFLRALDSRQVIVTFEVILSVRMVIT--KRQLSEIIWDLICDIMSSIVSNIEYYEEVNINKDRLHHLQINFHENIDCIEKLLQQDRSQILGNVERIYDLIERVADRRPEASVLALIEYRSRRVTATRPDWLQVLAQFVRRYYRMSN-VNVRIKTIEALVQIMDQNRACYEEEILSRVVLVHLSHIHLESSVQVRVAVARALSNFATHCDTKRCMDLLDILEALINRPFEH 542          

HSP 2 Score: 241.506 bits (615), Expect = 2.966e-63
Identity = 156/437 (35.70%), Postives = 228/437 (52.17%), Query Frame = 0
Query:  560 LIVVAEDESYHLGLEE-SDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPE-VFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE----NDKNKDSMGRMRSTSLSAARRPNVALAAKKDVAADVPKXTQIHP--LAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRC 988
            L+    + SYH+G  E S     FSPY+   SP       LL                     Q     +S+ + C L++ CLE +TD+ V  LV+  +P VLQN+ L+   G  I +    L+            ++N   K    +FH  + P +A+L  Y+E L P     +I AL   +L+   + +CI  +T   LEM  +    +P+VLL  SK+S T  ++TP+LEFLS ++ L + +F+ FT  + + VFAI LP+T P ++D YTVSLAHHVI  WFLKC+L  R+N V +I +S+ SN  +   +    N  N+DS  R RSTSL+     N A A       D+    Q++     FH EL ETC D LAR+ Y       +RL  ++F+LK+G S TWL+G  L+T++TS C     R+G+C+RC
Sbjct:  629 LLKARANGSYHIGYPEGSTEVVKFSPYLGIDSP-------LL--------------------PQAHPTAISIRRVCKLIVRCLEHDTDYQVFQLVIRELPKVLQNKALV--QGNDIEELANTLLKIN--------LVSNNKFKRPTDEFHALVLPAIASLVIYHESLQPQQHYGIITALNSRVLTGIAS-VCINTMTILILEMPEALMRKLPDVLLQMSKMSDTNALATPVLEFLSLLIHLSKHLFANFTSMHNMYVFAITLPYTKPHRYDHYTVSLAHHVIAGWFLKCKLELRRNCVNYIKSSIQSNAKMLSSDIVNLNSLNEDSSNRKRSTSLTERGSRNNANAWN-----DLEMRPQMNNGLRNFHAELAETCFDFLARHTYSPCPSMPKRLPAAEFLLKDGVSQTWLVGNNLVTITTSGCPSAPARSGLCERC 1022          

HSP 3 Score: 225.713 bits (574), Expect = 2.289e-58
Identity = 116/265 (43.77%), Postives = 168/265 (63.40%), Query Frame = 0
Query: 1276 ISPQFVFLMLYQSSTFGNAASLEKPLLL-PSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMP-NHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNLAAQIQKSLSNKMPYSSNWLZXLRAIKRLKEKL 1531
            ++P F+F+ LY   T G     ++PL + P N    +++  +D +P  ETHKIGVLY+G  Q N+EV ILRN  GS+RY +FL  +G L++++  +++  FI  LD    DG FAY+W DD++Q  FHVATLMP N  +DP  N+KK HIGN++  I+YN+S    E Y + T+ G+F  AC+++ PLD  SNRV V  + E+ + + H         S  +L RQ ALH NLA+ + +S+  K PY+SNWL+ LR +KRL+ KL
Sbjct: 1554 MNPSFIFMQLY---TTGQLGVTDEPLKVGPENS---SAVSLIDLVPPFETHKIGVLYVGQGQCNNEVEILRNSHGSARYVEFLRNIGTLVSLKEAEQNNLFI-MLDRNGADGKFAYIWKDDILQVTFHVATLMPTNLQDDPNCNEKKSHIGNDFVKIIYNES---GEEYNLNTISGQFNYACVIVEPLDLNSNRVYVKARSEISKFVCHAEYRIVSDRSAPLLARQMALHANLASLVYQSVQKKHPYASNWLERLRKLKRLRSKL 1808          
BLAST of EMLSAG00000002599 vs. Select Arthropod Genomes
Match: AGB94782.1 (gigas, isoform B [Drosophila melanogaster])

HSP 1 Score: 250.751 bits (639), Expect = 3.786e-66
Identity = 152/512 (29.69%), Postives = 272/512 (53.12%), Query Frame = 0
Query:   49 PLQERLKILKDLKES-VRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITPQLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHN---RIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLE-----KKLQDNISSHLHAVITILEGLSEMKRYS--GSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSLEQ 548
            P+ +R ++LK+L ++ + N  L +  + +L   T DL+  + P + R I LSFY+ L++ QY  L ++R  FF +I +NH  R   D  + ++LL  LT  GKDI  FE+++G F+   IP IT +      +L+++ N   FNA +LD D+++ ++     L  S         CL +L  +I Y+  P   L   I+ LCR VN   Y   ++ I + L+GT LG+ ++  +  I+       D  L+RGA+F +  +++G N   ++  + Y A  +L  F+ AL  +  +V FEVI  V+ ++T   ++L     D I +++  ++  +   E     K    ++  + H  I  +E L +  R    G+ ++++ +++  AD RPE SV+ LI +R + + A +  WL + ++ V RYY+    VN+RIKT++ ++ I   N   +EEE++ R+++  L  +H+E   ++R      L+   + C T + +DLL+IL  ++ R  E 
Sbjct:   39 PVAQRCRMLKELGDTQLHNFNLDESAITILFNLTNDLIVPNKPAETRQIALSFYKRLIHTQYKNLTIMREKFFLVI-QNHEAR--EDLRHLLELLDTLTDNGKDITNFEEKIGKFMLLWIPAIT-EANLLTPYLDILVNLIKFNAAHLDKDILVGIVQNACDLSCSVTVNEIGLQCLTILEMVIGYTIFPSEPLPQCITTLCRTVNHAPYCPSSFKIMKNLLGTQLGYHSMKMMCSILNDRALYDDAHLLRGAVFHLNMNIFGSNIIFQVSPMTY-ATNVLTAFLRALDSRQVIVTFEVILSVRMVIT--KRQLSEIIWDLICDIMSSIVSNIEYYEEVNINKDRLHHLQINFHENIDCIEKLLQQDRSQILGNVERIYDLIERVADRRPEASVLALIEYRSRRVTATRPDWLQVLAQFVRRYYRMSN-VNVRIKTIEALVQIMDQNRACYEEEILSRVVLVHLSHIHLESSVQVRVAVARALSNFATHCDTKRCMDLLDILEALINRPFEH 542          

HSP 2 Score: 238.81 bits (608), Expect = 2.279e-62
Identity = 157/437 (35.93%), Postives = 228/437 (52.17%), Query Frame = 0
Query:  560 LIVVAEDESYHLGLEE-SDGSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICRYGKSINKFITPLVSFTDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPE-VFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITS----LNSNIFVPFEENDKNKDSMGRMRSTSLSAARRPNVALAAKKDVAADVPKXTQIHP--LAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRC 988
            L+    + SYH+G  E S     FSPY+   SP       LL                     Q     +S+ + C L++ CLE +TD+ V  LV+  +P VLQN+ L+   G  I +    L+            ++N   K    +FH  + P +A+L  Y+E L P     +I AL   +L+   + +CI  +T   LEM  +    +P+VLL  SK+S T  ++TP+LEFLS ++ L + +F+ FT  + + VFAI LP+T P ++D YTVSLAHHVI  WFLKC+L  R+N V +I +S    L+S+I      N  N+DS  R RSTSL+     N A A       D+    Q++     FH EL ETC D LAR+ Y       +RL  ++F+LK+G S TWL+G  L+T++TS C     R+G+C+RC
Sbjct:  629 LLKARANGSYHIGYPEGSTEVVKFSPYLGIDSP-------LL--------------------PQAHPTAISIRRVCKLIVRCLEHDTDYQVFQLVIRELPKVLQNKALV--QGNDIEELANTLLKIN--------LVSNNKFKRPTDEFHALVLPAIASLVIYHESLQPQQHYGIITALNSRVLTGIAS-VCINTMTILILEMPEALMRKLPDVLLQMSKMSDTNALATPVLEFLSLLIHLSKHLFANFTSMHNMYVFAITLPYTKPHRYDHYTVSLAHHVIAGWFLKCKLELRRNCVNYIKSSNAKMLSSDIV---NLNSLNEDSSNRKRSTSLTERGSRNNANAWN-----DLEMRPQMNNGLRNFHAELAETCFDFLARHTYSPCPSMPKRLPAAEFLLKDGVSQTWLVGNNLVTITTSGCPSAPARSGLCERC 1019          

HSP 3 Score: 225.713 bits (574), Expect = 2.424e-58
Identity = 116/265 (43.77%), Postives = 168/265 (63.40%), Query Frame = 0
Query: 1276 ISPQFVFLMLYQSSTFGNAASLEKPLLL-PSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMP-NHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNLAAQIQKSLSNKMPYSSNWLZXLRAIKRLKEKL 1531
            ++P F+F+ LY   T G     ++PL + P N    +++  +D +P  ETHKIGVLY+G  Q N+EV ILRN  GS+RY +FL  +G L++++  +++  FI  LD    DG FAY+W DD++Q  FHVATLMP N  +DP  N+KK HIGN++  I+YN+S    E Y + T+ G+F  AC+++ PLD  SNRV V  + E+ + + H         S  +L RQ ALH NLA+ + +S+  K PY+SNWL+ LR +KRL+ KL
Sbjct: 1551 MNPSFIFMQLY---TTGQLGVTDEPLKVGPENS---SAVSLIDLVPPFETHKIGVLYVGQGQCNNEVEILRNSHGSARYVEFLRNIGTLVSLKEAEQNNLFI-MLDRNGADGKFAYIWKDDILQVTFHVATLMPTNLQDDPNCNEKKSHIGNDFVKIIYNES---GEEYNLNTISGQFNYACVIVEPLDLNSNRVYVKARSEISKFVCHAEYRIVSDRSAPLLARQMALHANLASLVYQSVQKKHPYASNWLERLRKLKRLRSKL 1805          
BLAST of EMLSAG00000002599 vs. nr
Match: gi|939636378|ref|XP_014294198.1| (PREDICTED: tuberin [Halyomorpha halys])

HSP 1 Score: 864.374 bits (2232), Expect = 0.000e+0
Identity = 569/1666 (34.15%), Postives = 879/1666 (52.76%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHG----EEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSPDVRHIVLSFYESLVYGQYSRLGLVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP--QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDN--------ISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRSL--------EQEAERDSLLKDLLIVVAEDESYHLG--------------LEESD-GSXXFSPYIICRSPEEEEEAEL---LWNRQQESASRPSSPSCLSGTRQKS-----------VLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLICR--YGKSINKFITPLVSFT-DKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAKKDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYC-IRDG--TLSKDDITKNSDRRRHMSDFYSGTNKSQLSNLRSDKELHRHTSLERMESSGDDYRVKKVLPETCSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNL-RELTALFDTSLKKDTFNFDVSTESDKI--------------NVDNLTE-DEYKKLSSEFVPSSVSS-------------PSAETDKTVSGNRSHDEEMIVMSPKSPVKKTKSSPPDTSCQPLEVSDIKRKKNQNNIPLNSSPNSSFESNLNEPAMRTTRSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNLAAQIQKSLS--NKMPYSSNWLZXLRAIKRLKEKLQKENEMKEE-EDREQGSGRPQLNDFTEF 1558
            MS   K  K   +KLKQ+F+  K    P      EE  L  E+   +S S +PL  R+K +++L E+V N +L++     L    +D+ R +S + RH V  F+ +++ GQY R+G++R+  F +IK NH+   S D    ++L   LT  GKDI+Y E+ +G F+   + ++TP   +   + +L ++  C  +NA+Y   D++  ++    +LC+ T ++    +CL++L+ I+CY+ +P   L  F+S LCR  N+E Y   +W I R L+GTHLGHSA+  +  ++Q+    +D  L+RGAIF+I  +LWG+ +I ++  + V +L     A+ CKH +++ EV+  ++++V      L       ++ ++  +++     +  +  N        + SHLH  +  +E L E  +++G  Q +F +++ C+  RPE SV RLI +  KSI  ++ +WL+  + L+ R+Y +++RV+IR K L ++     S  + FE +L  +I +  L +V  EP   IR    ++L  L   C+  + + ++  L  ++ R          E++AE   L    L+       + L               LEE        +P I  R    E   +L    + R   +         L  ++Q S           V  + +++A  +++  L+ E DW V+ ++L  +P +L++  L  +  Y   I+     + S   D   N P  LTNTP KF++SDFH+++FPV+ +L  Y+  L  A Q  LI+ LE GL  +   R C+ ALT   LEM+ +   L+PEVLLN SKISAT  I+ PILEFLST+ +LP+VF+ F    Y++VFAI+LP+TNPFK++ YTVSLAHHVI  WFLKCRL +R++FV+FIIT L +NIF+ FE+           N+DS  R RS+SL    S  R    +++++ D+   + +      ++FH+ELTETCLD++A Y +   S   +R   ++ IL  G S TWL+G  L+TV+TS CT  A+++G CD CY  C +R    T  K + +K  +     S   +  +        S+ E  + T   R E             + C+CWC  WAEI I+R +G VSW  R+ N +L S   P   +  E+ +     L   ++   VS  ++++              N+ +  E +E    S   V  S SS             P +E D T +  +    + +  + ++  ++  ++PP +    L   DI   K        S P S   S L     +  R HTISV SP  +       P +G    P        E     SG++P F FL LY S  FG A   EKP+L+ +    + ++KNLD IP +E HKIGVLY+ P Q   EV ILRN  GS+RY  FL+GLG+LIN+   D+   F+GGL+    DG FAY+W DDVMQ IFHVATLMPN+ NDP+ N+KK HIGNNY  IVYNDS    E Y   TV+ +F  A +VI PLD G+N+V V  K EL + + H         S++IL RQ A+H NLA+ I   L+  ++ PY+SNWL+ LR IKR++ KLQ+E    ++ + ++Q S R  ++DFTE+
Sbjct:    1 MSAKDKEVKTL-DKLKQFFRINKGA--PGSTKCKEEFSLPVEIEKYLS-SETPLHLRIKTVRELHETVLNNKLEENAPAKLWCLLEDIFREESKENRHAVFEFFRAVIQGQYERIGMIRAQVFKLIK-NHTI--SDDIVVRLELFQSLTDNGKDILYLEENVGMFL---LDWMTPIVNVGKEVDYLNILITCIKYNASYFHEDIICGIVQKTCALCSQTKEEQIVINCLDLLDIIVCYTNLPHESLPPFVSTLCRTANIELYCPLSWKIMRNLLGTHLGHSAMNTMCNLLQSRNTHTDPGLLRGAIFYINMALWGNKKIINIVCTPVNVLSALHTAIGCKHTVIMCEVMLAIQRMVKKQGPFLLDPTWAVVLRIMMYIVLNTENKKTIVDKNRPSLWTHLVFSHLHDTLNRIELLIEENKFNGRVQMVFDIVEKCSSSRPEISVERLIVYISKSISPSRCRWLDKLNSLMLRFYVQDERVSIRRKVLVILDSCIHSYRHMFESDLT-KIALDHLSNVANEPNLSIRTAGTQILVELFVNCEPARAMWVIEALEKVLNRPFSRVDFALTEEDAEDIKLAAHGLVNGLNASMFQLPPTPAIRAFNLITDHLEEHYLKPSLLAPVITVRYIMLECLLKLRADCYYRLGVAGQNKRHSIFLLASKQTSDIRNNVFQPNQVFTLDISRAVTMILTALKFENDWKVMLMLLQELPQMLKSCALFLQRDYSSDIDLIANTISSLVMDATWNLPDRLTNTPPKFTRSDFHSYVFPVVTSLIPYHGQLESALQQKLIKTLECGLAIRSA-RQCVFALTLCVLEMQDAMVKLLPEVLLNLSKISATVHIAIPILEFLSTLTQLPKVFANFVGDQYMSVFAISLPYTNPFKYNHYTVSLAHHVIAAWFLKCRLPFRRDFVKFIITGLKTNIFLCFEDLHLGVRSSELVNEDSSNRKRSSSLTEQGSRKRENRPSVSSRLDLKPPMDEAL----VSFHKELTETCLDLMAMYTFSTCSPIPKRSSCAEKILNGGQSMTWLVGNKLVTVTTSGCTLKALKHGFCDHCYQDCRVRPAPPTAMKKEDSKPPETIATSSTATTAPDGELPPVPPSNIEFPKVTGFHRPER------------QVCACWCKTWAEILIKRATGAVSWTMRLSN-ELHSLQCPKDYVTSEICSYTLPPLSSISYEDQVSVRNERVASEPVSIPCSPIRKNLHDFEEFEEGSGRSRNPVRRSNSSPEMSSNWKNPLLTPESEFDFTENKKKQTYSKDMRGNCEAIPEEMGTTPPTSEALMLAPCDIPDAK-------LSKPESD-PSGLPPLGFKRDRGHTISVMSPARK-------PGSGW-SDPRPRSPKPREITPTKSGVNPAFAFLQLYHSPYFGIAH--EKPILISNIPSVQATVKNLDWIPPYEVHKIGVLYVAPGQGTSEVEILRNEYGSARYMAFLEGLGSLINLTEADRQQVFLGGLETNGNDGKFAYIWQDDVMQVIFHVATLMPNNPNDPKGNRKKTHIGNNYVTIVYNDS---GEEYNFQTVKAQFNYAVVVIEPLDLGTNQVIVKAKEELHQHINHTEYKVVSDHSVAILARQLAVHSNLASMISCRLNSPSREPYASNWLERLRQIKRIRLKLQQEQGCGDDRQPKQQPSRRLLIDDFTEY 1616          
BLAST of EMLSAG00000002599 vs. nr
Match: gi|1035616060|ref|XP_016915834.1| (PREDICTED: tuberin isoform X1 [Apis cerana])

HSP 1 Score: 783.867 bits (2023), Expect = 0.000e+0
Identity = 476/1317 (36.14%), Postives = 711/1317 (53.99%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLG-LVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP-QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRS-------------------------------------------------LEQEAERDSLLKDL----------LIVVAEDESYHLGLEESD--GSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAK---KDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKD---------------DITKNSDRRRHMSDFYSGTNKSQLSN-----LRSDKE----LHRHTSLERME-SSGDDYRVKKVLPET-------CSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNLRELTALF----DTSLKKDTFNFDVSTE-----SDKINVDNLTEDE--YKKLSSEF------VPSSVSSPSAETDKTVSGNRS-HDEEMIVMSPKSPVKKTKSSP 1186
            MS   K  K   +KLKQ+F+  K N Y   E+  L+ +L   ISP  SP+  R K +KDL ++V N + +DK  E L Y  KDLL  D P + RH+  +F   LV GQYS+L  L+R  FF ++KE++      D    ++LL  LT  GKDI++ E+ +GPF+ + +P +T        +FL ++ N   FN+ Y+D D++  L+  I  LC  +N       CLE L++I+CYS +  + L  FI  LC  VN+E Y + +W I R L+GTH+GHSALY +  ++Q T  + DV L+RGA+F++   LWG ++I  L+ +  ++LP+F  ALKC H +V++EVI  +++LV  Y  EL       I+++IE ++       +     +S +LH  I  +E L ++  Y+G  Q+ + +++ C+D RPE SV++LI +R +SI      W    + L+ERYYK E R NIR+K LDV+ ++ + N   +EEELI+RII+P+   V M+ +  IR     +L  LC  C T + L+LL+IL  ++ +                                                  LEQ  +  ++  D+           + +  +  YHLG  ++       FSPY+I     E    E + +    ++  P +P+ L     + +  +SL  AC  VI C++ E DW VL LVL  +P V+QNR LI  R+   I+ F   L S  +DK+   P  L N P KF+ S+F  H+FPVLA+LASY+  L P  Q  LI+ LE+GL +K C   C+ +LTT  LEM+ +   L+ EVLLN SKISAT  I+ PILEFLST+ RLP+VF+ F    Y++VFAI LP+TNPFK+D YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N+ VPFEE        N  N+DS  R RS+SL    S  RR    +  +    + A+D+        + FH ELTETC+D++ARY +   S + +RL T+DF+LK G S TWL+G  LITV+TS C++ AMRNG+CD C+  C + G  S +               +I K     R  S  Y  +  +  +N     L+   E    + +  SLE+M+    +  +++++L          C+CWC GWAEIY+RRP+GD+SW+ R+QN+     +     + ++ AL+    D   K+  F  + S +     +DK N+D +  D    K + S        +P S + PS     +     S  D E  +   ++PV+++ SSP
Sbjct:    4 MSSKDKDNKTLHDKLKQFFRINKGN-YKSREDFTLTHDLEKEISPE-SPVHHRTKAIKDLCDAVLNQQWEDKAAERLWYLVKDLLEKDVPREHRHVTFNFLRCLVQGQYSKLSSLMRVKFFMVVKEHNIPE---DIGPRLELLQSLTENGKDILHLEERMGPFLLDWMPIVTAGDGKRGAEFLSLLVNVIKFNSAYIDEDIISGLVQYICHLCYYSNSSEVVSGCLEALDAIVCYSNLQSDSLQTFIIALCGSVNVETYCQISWKIMRNLLGTHMGHSALYTMCRLLQDTNFQRDVRLLRGAVFYVNMGLWGTHKIPKLECTPTSVLPSFYQALKCNHPVVMYEVILSIQRLVNKYGTELWDPTWSIILDIIEEVISHTETSNQPATRQVSVNLHETINSIENLLDINHYNGCIQRFYDLVERCSDARPESSVLKLIEYRARSIGPTHYHWQFKLANLMERYYKIETRTNIRMKVLDVLTNVVQINRSRYEEELIERIIVPYFQHVDMDFDITIRNGVAHLLIDLCLECDTKRCLELLDILEKVINKPFTSDIPVTKDIDIKDIKTAVVGVIKILISKIYYLPSSHAIRAYKVLVNYLEQHYKEPTIFYDIPTIRYLIFECFLKIRANTLYHLGFPDTQNLSVIKFSPYLIL----EHTTTERINSGGSGNSPPPVNPAPLQHLSCQ-ITYMSLALACKAVISCIKLEKDWKVLQLVLKELPQVMQNRALILSRHTNDIDYFANALCSMVSDKSLRLPESLYNVPPKFTLSEFRVHVFPVLASLASYHAHLEPNLQQRLIKCLEVGLTAK-CASQCVTSLTTCILEMRDAMNKLLSEVLLNLSKISATVHIAIPILEFLSTLTRLPKVFASFIGDQYMSVFAILLPYTNPFKYDHYTVSLAHHVIAVWFLKCRLPFRRDFVRFITTGLKANVIVPFEEGHLMKSDFNFINEDSSNRKRSSSLTEQGSRGRRERPIMTNRMIGDNKASDLKPPIDEALMTFHVELTETCIDLMARYTFSTYSARPKRLPTADFLLKEGQSMTWLLGNKLITVTTSGCSNKAMRNGLCDNCWVVC-KSGPQSPEHSKALQSNLRRASTIEIAKEDKLSRQFSGGYGSSTVNTAANSPTEELKKTSEDLDSIKKDHSLEKMDKDQAESSKLEQILNSEKQEEHVLCACWCQGWAEIYVRRPTGDMSWIMRIQNSMQFETNI-DFPVYDIMALYRPNQDLKQKQQEFTLEYSGDETEDIADK-NMDQIKNDHNNMKSMISSVSSGPIAIPGSPARPSPSRQSSRDSLESLEDGEDDLRRSRNPVRRSNSSP 1306          

HSP 2 Score: 275.789 bits (704), Expect = 3.180e-71
Identity = 152/346 (43.93%), Postives = 209/346 (60.40%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGE----KNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNLAAQIQKSLSNKM--PYSSNWLZXLRAIKRLKEKLQKEN-----EMKEEEDREQGSGRPQLNDFTEF 1558
            R HTISV SP  +              R     I  G     K    +GI+P FVFL LY ++ FG+ +  EKPLL+P     + ++ NLDRI  +ETHKIGVLY+GP Q ++E  IL N  GS RYT+FL  LG L+ ++++D+S  F+GGLD    +GNFAY+W DDV Q  FHVATLMP   +DP+   KK+HIGNNY  +VYN+S    ESY + TV+G+F  AC+VI PLD G+N+V V  K EL + + H         +L+IL RQ ALH NLA+ +  SL      PY+SNWL+ LR IKRL+ ++ +E+     +   ++   + + R  ++DFTE+
Sbjct: 1498 RGHTISVMSPVKK-------------SRGEWDNIRRGNSPRVKETPKTGINPSFVFLQLYHTAHFGSPS--EKPLLVPQTTAVQRAVTNLDRIQPYETHKIGVLYVGPGQASNETEILANQHGSLRYTEFLQRLGTLVRLKDVDESV-FLGGLDRNGENGNFAYIWQDDVTQVAFHVATLMPTKLSDPKCTSKKQHIGNNYVTVVYNES---GESYNIQTVKGQFNYACVVIQPLDHGTNQVTVQVKEELAKHIRHSEPKIISDQNLAILSRQLALHANLASMVSSSLEQNSHNPYASNWLERLRHIKRLRNRVLQESTNNNPDGTTDDLSPRSNKRIYMDDFTEY 1824          
BLAST of EMLSAG00000002599 vs. nr
Match: gi|380029021|ref|XP_003698181.1| (PREDICTED: LOW QUALITY PROTEIN: tuberin [Apis florea])

HSP 1 Score: 776.933 bits (2005), Expect = 0.000e+0
Identity = 469/1318 (35.58%), Postives = 702/1318 (53.26%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLG-LVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP-QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRS-------------------------------------------------LEQEAERDSLLKDL----------LIVVAEDESYHLGLEESD--GSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLC----VSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAK---KDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKD---------------DITKNSDRRRHMSDFYSGTN--------KSQLSNLRSDKELHRHTSLERME-SSGDDYRVKKVLPET-------CSCWCSGWAEIYIRRPSGDVSWMCRVQNT------------QLLSAHTPSTNLRELTALFDTSLKKDTFNFDVSTESDKINVD-NLTEDEYKKLSSE--FVPSSVSSPSAETDKTVSGNRS-HDEEMIVMSPKSPVKKTKSSP 1186
            MS   K  K   +KLKQ+F+  K N Y   E+  L+ +L   ISP  SP+  R K +KDL ++V N + +DK  E L Y  KDLL  D P + RH+ L+F   LV GQYS+L  L+R  FF ++KE++      D    ++LL  LT  GKDI++ ED +GPF+ + +P +T        +FL ++ N   FN+ Y+D D++  L+  I  LC  +N       CLE L++I+CYS +  + L  FI  LC  VN+E Y + +W I R L+GTH+GHSALY +  ++Q T  + DV L+RGA+F++   LWG ++I  L+ +  ++LP+F  ALKC H +V++EVI  +++LV  Y  EL       I+++IE ++       +     +S +LH  I  +E L ++  Y+G  Q+ + +++ C+D RPE SV++LI +R +SI      W    + L+ERYYK E R NIR+K LDV+ ++ + N   +EEELI+RII+P+   + M+ +  IR     +L  LC  C T + L+LL+IL  ++ +                                                  LEQ  +  ++  D+           + +  +  YHLG  ++       FSPY++      E         +  S    +SP  ++    + + C    +SL  AC  VI C++ E DW VL LVL  +P V+QNR LI  R+   I+ F   L S  +DK+   P  L N P KF+ S+F  H+FPVLA+LASY+  L P  Q  LI+ LE+GL +K C   C+ +LTT  LEM+ +   L+ EVLLN SKISAT  I+ PILEFLST+ RLP+VF+ F    Y++VFAI LP+TNPFK+D YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N+ VPFEE        N  N+DS  R RS+SL    S  RR    +  +    + A+D+        + FH ELTETC+D++ARY +   S + +RL  +DF+LK G S TWL+G  LITV+TS C++ AMRNG+CD C+  C + G  S +               +  K     R  S  Y  +           +L     D +  +  SLE+++    +  +++++L          C+CWC GWAEIY+RRP+GD+SW+ R+QN+             +++ + P+ +L++    F +    D          D+I  D N  +     +SS    +P S + PS     +     S  D E  +   ++PV+++ SSP
Sbjct:    4 MSSKDKDNKTLHDKLKQFFRINKGN-YKSREDFTLTHDLEKEISPE-SPVHHRTKAIKDLCDAVLNQQWEDKAAERLWYLVKDLLEKDVPREHRHVTLNFLRCLVQGQYSKLSSLMRVKFFMVVKEHNIPE---DIGPRLELLQSLTENGKDILHLEDVMGPFLLDWMPIVTAGDGKRGAEFLSLLVNVIKFNSAYIDEDIISGLVQYICHLCYYSNSSEVVSGCLEALDAIVCYSNLQSDSLQTFIIALCGSVNVETYCQISWKIMRNLLGTHMGHSALYTMCRLLQDTNFQRDVRLLRGAVFYVNMGLWGTHKIPKLECTPTSVLPSFYQALKCNHPVVMYEVILSIQRLVNKYGTELWDPTWSIILDIIEEVISHTETSNQPATKQVSVNLHETINSIENLLDINHYNGCIQRFYDLVERCSDARPEGSVLKLIEYRARSIGPTHYHWQFKLANLMERYYKIETRTNIRMKVLDVLTNVVQINRSRYEEELIERIIVPYFQHIDMDFDITIRNGVAHLLIDLCLECDTKRCLELLDILEKVINKPFTSDIPITKDVDIKDIKTAVVGVIKILISKIYYLPSSHAIRAYKVLVNYLEQHYKEPTVFYDIPTIRYLIFECFLKIRANTLYHLGFPDTQNLSMIKFSPYLVLEHTTTE---------RINSGGGGNSPPPINPVPLQHLSCQITHMSLALACKAVISCIKLEKDWKVLQLVLKELPQVMQNRALILSRHTNDIDYFANALCSMVSDKSLRLPESLYNVPPKFTLSEFRVHVFPVLASLASYHAHLEPNLQQRLIKCLEVGLTAK-CASQCVTSLTTCILEMRDAMNKLLSEVLLNLSKISATVHIAIPILEFLSTLTRLPKVFASFIGDQYMSVFAILLPYTNPFKYDHYTVSLAHHVIAVWFLKCRLPFRRDFVRFITTGLKANVIVPFEEGHLMKSDFNFINEDSSNRKRSSSLTEQGSRGRRERPIMTNRMIGDNKASDLKPPIDEALMTFHVELTETCIDLMARYTFSTYSARPKRLPAADFLLKEGQSMTWLLGNKLITVTTSGCSNKAMRNGLCDNCWVVC-KSGPQSPEHSKALQSNLRRASSIETAKEDKLSRQFSGGYGSSTVNTAANSPTEELKKTSEDLDSIKKDSLEKVDKDQAESSKLEQILNSEKQEEHVLCACWCQGWAEIYVRRPTGDMSWIMRIQNSMQFETNIDFPVYDIMALYRPNQDLKQKQQEFTSEYSGDETEDIADKNMDQIKNDHNNMKSMISSVSSGPIAIPGSPARPSPSRQSSRDSLESLEDGEDDLRRSRNPVRRSNSSP 1305          

HSP 2 Score: 276.559 bits (706), Expect = 1.974e-71
Identity = 154/345 (44.64%), Postives = 210/345 (60.87%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGE----KNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNLAAQIQKSL--SNKMPYSSNWLZXLRAIKRLKEKLQKE----NEMKEEEDREQGSGRPQLNDFTEF 1558
            R HTISV SP  +              R     I  G     K    +GI+P FVFL LY ++ FG+ +  EKPLL+P     + ++ NLDRI  +ETHKIGVLY+GP Q ++E  IL N  GS RYT+FL  LG L+ ++++D+S  F+GGLD    +GNFAY+W DDV Q  FHVATLMP   +DP+   KK+HIGNNY  +VYN+S    ESY + TV+G+F  AC+VI PLD G+N+V V  K EL + + H         +L+IL RQ ALH NLA+ +  SL  ++  PY+SNWL+ LR IKRL+ ++ +E    N    ++   + S R  ++DFTE+
Sbjct: 1496 RGHTISVMSPVKK-------------SRGEWDNIRRGNSPRIKETPKTGINPSFVFLQLYHTAHFGSPS--EKPLLVPQTTAVQRAVTNLDRIQPYETHKIGVLYVGPGQASNETEILANQHGSLRYTEFLQRLGTLVRLKDVDESV-FLGGLDRNGENGNFAYIWQDDVTQVAFHVATLMPTKLSDPKCTSKKQHIGNNYVTVVYNES---GESYNIQTVKGQFNYACVVIQPLDHGTNQVTVQVKEELAKHIRHSEPKIISDQNLAILSRQLALHANLASMVSSSLEQNSHNPYASNWLERLRHIKRLRNRVLQESTNNNPDGTDDLSPRLSKRIYMDDFTEY 1821          
BLAST of EMLSAG00000002599 vs. nr
Match: gi|572315473|ref|XP_006623386.1| (PREDICTED: tuberin-like isoform X1 [Apis dorsata])

HSP 1 Score: 776.548 bits (2004), Expect = 0.000e+0
Identity = 472/1315 (35.89%), Postives = 710/1315 (53.99%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLG-LVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP-QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRS-------------------------------------------------LEQEAERDSLLKDL----------LIVVAEDESYHLGLEESD--GSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAK---KDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKD---------------DITKNSDRRRHMSDFYSGTNKSQLSN-----LRSDKE----LHRHTSLERME-SSGDDYRVKKVLPET-------CSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNLRELTALF--DTSLKKDTFNFDVSTE-----SDKINVDNLTEDE--YKKLSSEF------VPSSVSSPSAETDKTVSGNRS-HDEEMIVMSPKSPVKKTKSSP 1186
            MS   K  K   +KLKQ+F+  K N Y   E+  L+ +L   ISP  SP+  R K +KDL ++V N + +DK  E L Y  KDLL  D P + RH+ L+F   LV GQYS+L  L+R  FF ++KE++      D    ++LL  LT  GKDI++ E+ +GPF+ + +P +T        +FL ++ N   FN+ Y+D D++  L+  I  LC  +N       CLE L++I+CYS +  + L  FI  LC  VN+E Y + +W I R L+GTH+GHSALY +  ++Q T  + DV L+RGA+F++   LWG ++I  L+ +  ++LP+F  ALKC H +V++EVI  +++LV  Y  EL       I+++IE ++       +     +S +LH  I  +E L ++  Y+G  Q+ + +++ C+D RPE SV++LI +R +SI      W    + L+ERYYK E R NIR+K LDV+ ++ + N   +EEELI+RII+P+   V M+ +  IR     +L  LC  C T + L+LL+IL  ++ +                                                  LEQ  +  ++  D+           + +  +  YHLG  ++       FSPY++     E    E + +    ++  P +P+ L     + +  +SL  AC  VI C++ E DW VL LVL  +P V+QNR LI  R+   I+ F   L S  +DK+   P  L N P KF+ S+F  H+FPVLA+LASY+  L P  Q  LI+ LE+GL +K C   C+ +LTT  LEM+ +   L+ EVLLN SKISAT  I+ PILEFLST+ RLP+VF+ F    Y++VFAI LP+TNPFK+D YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N+ VPFEE        N  N+DS  R RS+SL    S  RR    +  +    + A+D+        + FH ELTETC+D++ARY +   S + +RL  +DF+LK   S TWL+G  LITV+TS C++ AMRNG+CD C+  C + G  S +               +  K     R  S  Y  +  +  +N     L+   E    + +   LE+M+    +  +++++L          C+CWC GWAEIY+RRP+GD+SW+ R+QN+     +     + ++ AL+  + +LK+  F  + S +     +DK N+D +  D    K + S        +P S + PS     +     S  D E  +   ++PV+++ SSP
Sbjct:    4 MSSKDKDNKTLHDKLKQFFRINKGN-YKSREDFTLTHDLEKEISPE-SPVHHRTKAIKDLCDAVLNQQWEDKAAERLWYLVKDLLEKDIPREHRHVTLNFLRCLVQGQYSKLSSLMRVKFFMVVKEHNIPE---DIGPRLELLQSLTENGKDILHLEERMGPFLLDWMPIVTAGDGKRGAEFLSLLVNVIKFNSAYIDEDIISGLVQYICHLCYYSNSSEVVSGCLEALDAIVCYSNLQSDSLQTFIIALCGSVNVETYCQISWKIMRNLLGTHMGHSALYTMCRLLQDTNFQRDVRLLRGAVFYVNMGLWGTHKIPKLECTPTSVLPSFYQALKCNHPVVMYEVILSIQRLVNKYGTELWDPTWSIILDIIEEVISHTETSNQPATRQVSVNLHETINSIENLLDINHYNGCIQRFYDLVERCSDARPEGSVLKLIEYRARSIGPTHYHWQFKLANLMERYYKIETRTNIRMKVLDVLTNVVQINRSRYEEELIERIIVPYFQHVDMDFDITIRNGVAHLLIDLCLECDTKRCLELLDILEKVINKPFTSDIPVTKDVDIKDIKTAVVGVIKILISKIYYLPSSHAIRAYKVLVNYLEQHYKEPTVFYDIPTIRYLIFECFLKIRANTLYHLGFPDTQNLSVIKFSPYLVL----EHTTIERINSGGGGNSPPPVNPTPLQHLSCQ-ITYMSLALACKAVISCIKLEKDWKVLQLVLKELPQVMQNRALILSRHTNDIDYFANALCSMVSDKSLRLPESLYNVPPKFTLSEFRVHVFPVLASLASYHAHLEPNLQQRLIKCLEVGLTAK-CASQCVTSLTTCILEMRDAMNKLLSEVLLNLSKISATVHIAIPILEFLSTLTRLPKVFASFIGDQYMSVFAILLPYTNPFKYDHYTVSLAHHVIAVWFLKCRLPFRRDFVKFITTGLKANVIVPFEEGHLMKSDFNFINEDSSNRKRSSSLTEQGSRGRRERPIMTNRMIGDNKASDLKPPIDEALMTFHVELTETCIDLMARYTFSTYSARPKRLPAADFLLKEAQSMTWLLGNKLITVTTSGCSNKAMRNGLCDNCWVVC-KSGPQSPEHSKALQSNLRRASSIETAKEDKLSRQFSGGYGSSTVNTAANSPTEELKKTSEDLDSIKKDHPLEKMDKDQAESSKLEQILNSEKQEEHVLCACWCQGWAEIYVRRPTGDMSWIMRIQNSMQFETNI-DFPVYDIMALYRPNQNLKQQEFTSEYSGDETEDIADK-NMDQIKNDHNNMKSMISSVSSGPIAIPGSPARPSPSKQSSRDSLESLEDGEDDLRRSRNPVRRSNSSP 1304          

HSP 2 Score: 275.018 bits (702), Expect = 6.001e-71
Identity = 151/346 (43.64%), Postives = 210/346 (60.69%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGE----KNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNLAAQIQKSLSNKM--PYSSNWLZXLRAIKRLKEKLQKEN-----EMKEEEDREQGSGRPQLNDFTEF 1558
            R HTISV SP  +              R     I  G     K+   +GI+P FVFL LY ++ FG+ +  EKPLL+P     + ++ NLDRI  +ETHKIGVL++GP Q ++E  IL N  GS RYT+FL  LG L+ ++++D+S  F+GGLD    +GNFAY+W DDV Q  FHVATLMP   +DP+   KK+HIGNNY  +VYN+S    ESY + TV+G+F  AC+VI PLD G+N+V V  K EL + + H         +L+IL RQ ALH NLA+ +  SL      PY+SNWL+ LR IKRL+ ++ +E+     +   ++   + + R  ++DFTE+
Sbjct: 1496 RGHTISVMSPVKK-------------SRGEWDNIRRGNSPRVKDTPKTGINPSFVFLQLYHTAHFGSPS--EKPLLVPQTTAVQRAVTNLDRIQPYETHKIGVLFVGPGQASNETEILANQHGSLRYTEFLQRLGTLVRLKDVDESV-FLGGLDRNGENGNFAYIWQDDVTQVAFHVATLMPTKLSDPKCTSKKQHIGNNYVTVVYNES---GESYNIQTVKGQFNYACVVIQPLDHGTNQVTVQVKEELAKHIRHSEPKIISDQNLAILSRQLALHANLASMVSSSLEQNSHNPYASNWLERLRHIKRLRNRVLQESTNNNPDGTTDDLSPRSNKRIYMDDFTEY 1822          
BLAST of EMLSAG00000002599 vs. nr
Match: gi|571577989|ref|XP_006572241.1| (PREDICTED: tuberin isoform X1 [Apis mellifera])

HSP 1 Score: 775.778 bits (2002), Expect = 0.000e+0
Identity = 469/1315 (35.67%), Postives = 706/1315 (53.69%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLG-LVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP-QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRS-------------------------------------------------LEQEAERDSLLKDL----------LIVVAEDESYHLGLEESD--GSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAK---KDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKD---------------DITKNSDRRRHMSDFYSGTNKSQLSN-----LRSDKE----LHRHTSLERME-SSGDDYRVKKVLPET-------CSCWCSGWAEIYIRRPSGDVSWMCRVQNT------------QLLSAHTPSTNLRELTALFDTSLKKDTFNFDVSTESDKINVD-NLTEDEYKKLSSE--FVPSSVSSPSAETDKTVSGNRS-HDEEMIVMSPKSPVKKTKSSP 1186
            MS   K  K   +KLKQ+F+  K N Y   E+  L+ +L   ISP  SP+  R K +KDL ++V N + +DK  E L Y  KDLL  D P + RH+  +F   LV GQYS+L  L+R  FF ++KE++      D    ++LL  LT  GKDI++ E+ +GPF+ + +P +T        +FL ++ N   FN+ Y+D D++  L+  I  LC  +N       CLE L++I+CYS +  + L  FI  LC  VN+E Y + +W I R L+GTH+GHSALY +  ++Q T  + DV L+RGA+F++   LWG ++I  L+ +  ++LP+F  ALKC H +V++EVI  +++LV  Y  EL       I+++IE ++       +     +S +LH  I  +E L ++  Y+G  Q+ + +++ C+D RPE SV++LI +R +SI      W    + L+ERYYK E R NIR+K LDV+ ++ + N   +EEELI+RII+P+   V M+ +  IR     +L  LC  C T + L+LL+IL  ++ +                                                  LEQ  +  ++  D+           + +  +  YHLG  ++       FSPY+I     E    E + +    ++  P +P+ L     + +  +SL  AC  VI C++ E DW VL LVL  +P V+QNR LI  R+   I+ F   L S  +DK+   P  L N P KF+ S+F  H+FPVLA+LASY+  L P  Q  LI+ LE+GL +K C   C+ +LTT  LEM+ +   L+ EVLLN SKISAT  I+ PILEFLST+ RLP+VF+ F    Y++VFAI LP+TNPFK+D YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N+ VPFEE        N  N+DS  R RS+SL    S  RR    +  +    +  +D+        + FH ELTETC+D++ARY +   S + +RL ++DF+LK G S TWL+G  LITV+TS C++ AMRNG+CD C+  C + G  S +               +  K     R  S  Y  +  +  +N     L+   E    + +  SLE+++    +  +++++L          C+CWC GWAEIY+RRP+GD+SW+ R+QN+             +++ + P+ +L++    F +    D          D+I  D N  +     +SS    +P S + PS     +     S  D E  +   ++PV+++ SSP
Sbjct:    4 MSSKDKDNKTLHDKLKQFFRINKGN-YKSREDFTLTHDLEKEISPE-SPVHHRTKAIKDLCDAVLNQQWEDKAAERLWYLVKDLLEKDVPREHRHVTFNFLRCLVQGQYSKLSSLMRVKFFMVVKEHNIPE---DIGPRLELLQSLTENGKDILHLEERMGPFLLDWMPIVTAGDGKRGAEFLLLLVNVIKFNSAYIDEDIISGLVQYICHLCYYSNSSEVVSGCLEALDAIVCYSNLQSDSLQTFIIALCGSVNVETYCQISWKIMRNLLGTHMGHSALYTMCRLLQDTNFQRDVRLLRGAVFYVNMGLWGTHKIPKLECTPTSVLPSFYQALKCNHPVVMYEVILSIQRLVNKYGTELWDPTWSIILDIIEEVISHTETSNQPATRQVSVNLHETINSIENLLDINHYNGCIQRFYDLVERCSDARPESSVLKLIEYRARSIGPTHYHWQFKLANLMERYYKIETRTNIRMKVLDVLTNVVQINRSRYEEELIERIIVPYFQHVDMDFDITIRNGVAHLLIDLCLECDTKRCLELLDILEKVINKPFTSDIPITKDVDIKDIKTAVVGVIKILISKIYYLPSSHAIRAYKVLVNYLEQHYKEPTVFYDIPTIRYLIFECFLKIRANTLYHLGFPDTQNLSVIKFSPYLIL----EHTTTERINSGGGGNSPPPVNPAPLQHLSCQ-ITYMSLALACKAVISCIKLEKDWKVLQLVLKELPQVMQNRALILSRHTNDIDYFANALCSMVSDKSLRLPESLYNVPPKFTLSEFRVHVFPVLASLASYHAHLEPNLQQRLIKCLEVGLTAK-CASQCVTSLTTCILEMRDAMNKLLSEVLLNLSKISATVHIAIPILEFLSTLTRLPKVFASFIGDQYMSVFAILLPYTNPFKYDHYTVSLAHHVIAVWFLKCRLPFRRDFVRFITTGLKANVIVPFEEGHLMKSDFNFINEDSSNRKRSSSLTEQGSRGRRERPIMTNRMIGDNKVSDLKPPIDEALMTFHVELTETCIDLMARYTFSTYSARPKRLPSADFLLKEGQSMTWLLGNKLITVTTSGCSNKAMRNGLCDNCWVVC-KSGPQSPEHSKALQSNLRRASSIETAKEDKLSRQFSGGYGNSTVNTAANSPTEELKKTSEDLDSIKKDHSLEKIDKDQAESSKLEQILNSEKQEEHVLCACWCQGWAEIYVRRPTGDMSWIMRIQNSMQFETNIDFPVYDIMALYRPNQDLKQKQQEFTSEYSGDETEDIADKNMDQIKNDHNNMKSMISSVSSGPIAIPGSPARPSPSRQSSRDSLESLEDGEDDLRRSRNPVRRSNSSP 1306          

HSP 2 Score: 275.789 bits (704), Expect = 3.180e-71
Identity = 152/346 (43.93%), Postives = 209/346 (60.40%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGE----KNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNLAAQIQKSLSNKM--PYSSNWLZXLRAIKRLKEKLQKEN-----EMKEEEDREQGSGRPQLNDFTEF 1558
            R HTISV SP  +              R     I  G     K    +GI+P FVFL LY ++ FG+ +  EKPLL+P     + ++ NLDRI  +ETHKIGVLY+GP Q ++E  IL N  GS RYT+FL  LG L+ ++++D+S  F+GGLD    +GNFAY+W DDV Q  FHVATLMP   +DP+   KK+HIGNNY  +VYN+S    ESY + TV+G+F  AC+VI PLD G+N+V V  K EL + + H         +L+IL RQ ALH NLA+ +  SL      PY+SNWL+ LR IKRL+ ++ +E+     +   ++   + + R  ++DFTE+
Sbjct: 1498 RGHTISVMSPVKK-------------SRGEWDNIRRGNSPRIKETPKTGINPSFVFLQLYHTAHFGSPS--EKPLLVPQTTAVQRAVTNLDRIQPYETHKIGVLYVGPGQASNETEILANQHGSLRYTEFLQRLGTLVRLKDVDESV-FLGGLDRNGENGNFAYIWQDDVTQVAFHVATLMPTKLSDPKCTSKKQHIGNNYVTVVYNES---GESYNIQTVKGQFNYACVVIQPLDHGTNQVTVQVKEELAKHIRHSEPKIISDQNLAILSRQLALHANLASMVSSSLEQNSHNPYASNWLERLRHIKRLRNRVLQESTNNNPDGTTDDLSPRSNKRIYMDDFTEY 1824          
BLAST of EMLSAG00000002599 vs. nr
Match: gi|1031995841|ref|XP_016772912.1| (PREDICTED: tuberin isoform X2 [Apis mellifera])

HSP 1 Score: 775.393 bits (2001), Expect = 0.000e+0
Identity = 469/1315 (35.67%), Postives = 706/1315 (53.69%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLG-LVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP-QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRS-------------------------------------------------LEQEAERDSLLKDL----------LIVVAEDESYHLGLEESD--GSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAK---KDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKD---------------DITKNSDRRRHMSDFYSGTNKSQLSN-----LRSDKE----LHRHTSLERME-SSGDDYRVKKVLPET-------CSCWCSGWAEIYIRRPSGDVSWMCRVQNT------------QLLSAHTPSTNLRELTALFDTSLKKDTFNFDVSTESDKINVD-NLTEDEYKKLSSE--FVPSSVSSPSAETDKTVSGNRS-HDEEMIVMSPKSPVKKTKSSP 1186
            MS   K  K   +KLKQ+F+  K N Y   E+  L+ +L   ISP  SP+  R K +KDL ++V N + +DK  E L Y  KDLL  D P + RH+  +F   LV GQYS+L  L+R  FF ++KE++      D    ++LL  LT  GKDI++ E+ +GPF+ + +P +T        +FL ++ N   FN+ Y+D D++  L+  I  LC  +N       CLE L++I+CYS +  + L  FI  LC  VN+E Y + +W I R L+GTH+GHSALY +  ++Q T  + DV L+RGA+F++   LWG ++I  L+ +  ++LP+F  ALKC H +V++EVI  +++LV  Y  EL       I+++IE ++       +     +S +LH  I  +E L ++  Y+G  Q+ + +++ C+D RPE SV++LI +R +SI      W    + L+ERYYK E R NIR+K LDV+ ++ + N   +EEELI+RII+P+   V M+ +  IR     +L  LC  C T + L+LL+IL  ++ +                                                  LEQ  +  ++  D+           + +  +  YHLG  ++       FSPY+I     E    E + +    ++  P +P+ L     + +  +SL  AC  VI C++ E DW VL LVL  +P V+QNR LI  R+   I+ F   L S  +DK+   P  L N P KF+ S+F  H+FPVLA+LASY+  L P  Q  LI+ LE+GL +K C   C+ +LTT  LEM+ +   L+ EVLLN SKISAT  I+ PILEFLST+ RLP+VF+ F    Y++VFAI LP+TNPFK+D YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N+ VPFEE        N  N+DS  R RS+SL    S  RR    +  +    +  +D+        + FH ELTETC+D++ARY +   S + +RL ++DF+LK G S TWL+G  LITV+TS C++ AMRNG+CD C+  C + G  S +               +  K     R  S  Y  +  +  +N     L+   E    + +  SLE+++    +  +++++L          C+CWC GWAEIY+RRP+GD+SW+ R+QN+             +++ + P+ +L++    F +    D          D+I  D N  +     +SS    +P S + PS     +     S  D E  +   ++PV+++ SSP
Sbjct:    4 MSSKDKDNKTLHDKLKQFFRINKGN-YKSREDFTLTHDLEKEISPE-SPVHHRTKAIKDLCDAVLNQQWEDKAAERLWYLVKDLLEKDVPREHRHVTFNFLRCLVQGQYSKLSSLMRVKFFMVVKEHNIPE---DIGPRLELLQSLTENGKDILHLEERMGPFLLDWMPIVTAGDGKRGAEFLLLLVNVIKFNSAYIDEDIISGLVQYICHLCYYSNSSEVVSGCLEALDAIVCYSNLQSDSLQTFIIALCGSVNVETYCQISWKIMRNLLGTHMGHSALYTMCRLLQDTNFQRDVRLLRGAVFYVNMGLWGTHKIPKLECTPTSVLPSFYQALKCNHPVVMYEVILSIQRLVNKYGTELWDPTWSIILDIIEEVISHTETSNQPATRQVSVNLHETINSIENLLDINHYNGCIQRFYDLVERCSDARPESSVLKLIEYRARSIGPTHYHWQFKLANLMERYYKIETRTNIRMKVLDVLTNVVQINRSRYEEELIERIIVPYFQHVDMDFDITIRNGVAHLLIDLCLECDTKRCLELLDILEKVINKPFTSDIPITKDVDIKDIKTAVVGVIKILISKIYYLPSSHAIRAYKVLVNYLEQHYKEPTVFYDIPTIRYLIFECFLKIRANTLYHLGFPDTQNLSVIKFSPYLIL----EHTTTERINSGGGGNSPPPVNPAPLQHLSCQ-ITYMSLALACKAVISCIKLEKDWKVLQLVLKELPQVMQNRALILSRHTNDIDYFANALCSMVSDKSLRLPESLYNVPPKFTLSEFRVHVFPVLASLASYHAHLEPNLQQRLIKCLEVGLTAK-CASQCVTSLTTCILEMRDAMNKLLSEVLLNLSKISATVHIAIPILEFLSTLTRLPKVFASFIGDQYMSVFAILLPYTNPFKYDHYTVSLAHHVIAVWFLKCRLPFRRDFVRFITTGLKANVIVPFEEGHLMKSDFNFINEDSSNRKRSSSLTEQGSRGRRERPIMTNRMIGDNKVSDLKPPIDEALMTFHVELTETCIDLMARYTFSTYSARPKRLPSADFLLKEGQSMTWLLGNKLITVTTSGCSNKAMRNGLCDNCWVVC-KSGPQSPEHSKALQSNLRRASSIETAKEDKLSRQFSGGYGNSTVNTAANSPTEELKKTSEDLDSIKKDHSLEKIDKDQAESSKLEQILNSEKQEEHVLCACWCQGWAEIYVRRPTGDMSWIMRIQNSMQFETNIDFPVYDIMALYRPNQDLKQKQQEFTSEYSGDETEDIADKNMDQIKNDHNNMKSMISSVSSGPIAIPGSPARPSPSRQSSRDSLESLEDGEDDLRRSRNPVRRSNSSP 1306          

HSP 2 Score: 241.121 bits (614), Expect = 1.636e-60
Identity = 130/280 (46.43%), Postives = 171/280 (61.07%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGE----KNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNL 1499
            R HTISV SP  +              R     I  G     K    +GI+P FVFL LY ++ FG+ +  EKPLL+P     + ++ NLDRI  +ETHKIGVLY+GP Q ++E  IL N  GS RYT+FL  LG L+ ++++D+S  F+GGLD    +GNFAY+W DDV Q  FHVATLMP   +DP+   KK+HIGNNY  +VYN+S    ESY + TV+G+F  AC+VI PLD G+N+V V  K EL + + H         +L+IL RQ ALH N+
Sbjct: 1498 RGHTISVMSPVKK-------------SRGEWDNIRRGNSPRIKETPKTGINPSFVFLQLYHTAHFGSPS--EKPLLVPQTTAVQRAVTNLDRIQPYETHKIGVLYVGPGQASNETEILANQHGSLRYTEFLQRLGTLVRLKDVDESV-FLGGLDRNGENGNFAYIWQDDVTQVAFHVATLMPTKLSDPKCTSKKQHIGNNYVTVVYNES---GESYNIQTVKGQFNYACVVIQPLDHGTNQVTVQVKEELAKHIRHSEPKIISDQNLAILSRQLALHANV 1758          
BLAST of EMLSAG00000002599 vs. nr
Match: gi|815895494|ref|XP_012243202.1| (PREDICTED: tuberin [Bombus impatiens])

HSP 1 Score: 775.007 bits (2000), Expect = 0.000e+0
Identity = 467/1317 (35.46%), Postives = 700/1317 (53.15%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLG-LVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP-QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRS-------------------------------------------------LEQEAERDSLLKDL----------LIVVAEDESYHLGLEESDGSX--XFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQK---SVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAK---KDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKDDITKNSDRRR----------HMSDFYSGTNKSQLSNLRS------------DKELHR--HTSLERMESSGDDYRVKKVLPET-------CSCWCSGWAEIYIRRPSGDVSWMCRVQNT------------QLLSAHTPSTNLRELTALFDTSLKKDTFNFDVSTESDKINVD-NLTEDEYKKLSSE--FVPSSVSSPSAETDKTVSGNRS-HDEEMIVMSPKSPVKKTKSSP 1186
            M+   K  K   +KLKQ+F+  K N Y   E++ L+ +L   ISP  SP+  R K++KDL ++V N + +DK  E L Y  +DLL  D P + RH+ L+F   LV GQYS+L  L+R  FF ++KE++      D    +DLL  LT  GKDI++ E+ +GPF+ + +P +T        +FL ++ N   FN+ Y+D D++  L+  I  LC  +N       CLE L++I+CYS +  + L  FI  LC  VN+E Y + +W I R L+GTH+GHSALY +  ++Q T  + DV L+RGA+F++   LWG +RI  L+ +  ++LP+F  ALKC H +V++EV   +++LV  Y  EL       I+++IE ++       +     +S  LH  I  +E L +   Y+G  Q+ + +++ C+D RPE S+++LI +R ++I A    W +  + L+ERYYK E R N+R+K LDV+ ++ + N   +E+ELI+RII+P+   V M+ +  IR     +L  LC  C T + L+LL+IL  ++ +                                                  LEQ  +  ++  D+           + +  +  YHLG  +   +    FSPY++      E        R        S P       Q     +  +SL  AC  VI C++ E DW VL LVL  +P V+QNR LI  R+   I+ F T L S  +DK+   P  L N P KF+ S+F  H+FPVLA+LASY+  L P  Q  LI+ LE+GL +K C   C+ +LTT  LEM+ +   L+ EVLLN SKISAT  I+ PILEFLST+ RLP+VF+ F    Y++VFAI LP+TNPFK+D YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N+ VPFEE        N  N+DS  R RS+SL    S  RR    +A +    +  +D+        + FH ELTETC+D++ARY +   S + +RL  +DF+LK G S TWL+G  LITV+TS C++ AMRNG+CD C+  C       +   T  S  RR           +S   SG + S  +N  +            D +L +  H+S +  +   +  +++++L          C+CWC GWAEIY+RRP+GD+SW+ R+QN+             +++ + P+ +L +      +    D          D+I  D N+ +     +SS    +P S + PS     +     S  D E  +   ++PV+++ SSP
Sbjct:    4 MNSKDKDNKTLHDKLKQFFRINKGN-YKSREDVTLTHDLEKEISPE-SPVHHRTKVIKDLCDAVLNQQWEDKAAERLWYLVQDLLEKDVPREHRHVTLNFLRCLVQGQYSKLSSLMRVKFFMVVKEHNIPE---DIGPRLDLLQSLTENGKDILHLEERMGPFLLDWMPVVTAGDGKRGAEFLSLLVNVIKFNSAYIDEDIISGLVQYICHLCYYSNSSEVVSGCLEALDAIVCYSNLQSDSLQTFIIALCGSVNVETYCQISWKIMRNLLGTHMGHSALYTMCRLLQDTNFQRDVRLLRGAVFYVNMGLWGTHRIPKLECTPTSVLPSFYQALKCNHPIVMYEVTLSIQRLVNKYGAELWDPTWSIILDIIEEVISHTETSNQPATRQVSVSLHETINSIENLLDSNYYNGCIQRFYDLVERCSDARPEGSILKLIEYRARTIGATHHLWQSRLANLMERYYKIETRTNVRMKVLDVLTNVVQVNRSRYEDELIERIIVPYFQHVDMDSDITIRNGVAHLLVDLCLECDTKRCLELLDILEKVINKPFTSDTPVMKDVDIKDVKTAIVGVIKILTSKIYYLPSSHAIRAYKVLVNHLEQHYKEPTVFHDISTIRYLIFECFLKIRANTLYHLGFPDPQNASVIKFSPYLVLEHATTE--------RMNSGGGGSSPPPVSPAPLQHLSCQITHMSLAHACKAVISCIKSEKDWKVLQLVLKELPQVMQNRALILSRHTNDIDFFATALCSMVSDKSLRLPESLYNVPPKFTLSEFRVHVFPVLASLASYHAHLEPNLQQRLIKCLEVGLTAK-CASQCVTSLTTCILEMRDAMNKLLSEVLLNLSKISATVHIAIPILEFLSTLTRLPKVFASFIGDQYMSVFAILLPYTNPFKYDHYTVSLAHHVIAVWFLKCRLPFRRDFVRFITTGLKANVIVPFEEGHLMKSDFNFINEDSSNRKRSSSLTEQGSRGRRERPIMANRMIGDNKVSDLKPPIDEALMTFHVELTETCIDLMARYTFSTYSARPKRLAAADFLLKEGQSMTWLLGNKLITVTTSGCSNKAMRNGLCDNCWVVCKPGPQSPEHSKTSQSTLRRASSTETAKEDKLSRQSSGGHGSSTANTAANSPTEESKKASEDLDLTKKDHSSEKMEKDQAESSKLEQILNSEKQEEHVLCACWCQGWAEIYVRRPTGDMSWIMRIQNSMQFETNVDFPVYDIMALYRPNQDLLQKQQESTSECSGDEAEDTADKSMDQIRCDHNIMKSVISSVSSGPIAIPGSPARPSPSRQSSRDSLESLEDGEDDLRRSRNPVRRSNSSP 1306          

HSP 2 Score: 273.092 bits (697), Expect = 2.715e-70
Identity = 151/341 (44.28%), Postives = 207/341 (60.70%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNLAAQIQKSLSNKM--PYSSNWLZXLRAIKRLKEKLQKENEMKEEEDREQGSGRPQ----LNDFTEF 1558
            R HTISV SP        ++ + G       G     ++  K S I+P FVFL LY ++ FG+ +  EKPLL+P     + ++ NLDRI  +ETHKIGVLY+GP Q ++E  IL N  GS RYT+FL  LG L+ ++++D+S  F+GGLD    +GNFAY+W DDV Q  FHVATLMP   +DP+   KK+HIGNNY  +VYN+S    E Y + TV+G+F  AC+VI PLD G+N+V V  K EL + + H         +L+IL RQ ALH NLA+ +  SL      PY+SNWL+ LR IKRL+ ++ +E     +   +  S R      ++DFTE+
Sbjct: 1500 RGHTISVMSP--------VKKSRGEWDNIRRGNSPRVKETPKTS-INPSFVFLQLYHTAHFGSPS--EKPLLVPQTTAVQRAVTNLDRIQPYETHKIGVLYVGPGQASNETEILANQHGSLRYTEFLQRLGTLVRLKDVDESV-FLGGLDRNGENGNFAYIWQDDVTQVAFHVATLMPTKLSDPKCTSKKQHIGNNYVTVVYNES---GEPYNIQTVKGQFNYACVVIQPLDHGTNQVTVQVKEELVKHIRHSEPKIISDQNLAILSRQLALHANLASMVSSSLEQNSHNPYASNWLERLRHIKRLRNRVLQETNNNPDRTTDDLSPRSNKRIYMDDFTEY 1825          
BLAST of EMLSAG00000002599 vs. nr
Match: gi|572315475|ref|XP_006623387.1| (PREDICTED: tuberin-like isoform X2 [Apis dorsata])

HSP 1 Score: 774.622 bits (1999), Expect = 0.000e+0
Identity = 470/1315 (35.74%), Postives = 710/1315 (53.99%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLG-LVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP-QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRS-------------------------------------------------LEQEAERDSLLKDL----------LIVVAEDESYHLGLEESD--GSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAK---KDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKD---------------DITKNSDRRRHMSDFYSGTNKSQLSNLRSDK---------ELHRHTSLERME-SSGDDYRVKKVLPET-------CSCWCSGWAEIYIRRPSGDVSWMCRVQNTQLLSAHTPSTNLRELTALF--DTSLKKDTFNFDVSTE-----SDKINVDNLTEDE--YKKLSSEF------VPSSVSSPSAETDKTVSGNRS-HDEEMIVMSPKSPVKKTKSSP 1186
            MS   K  K   +KLKQ+F+  K N Y   E+  L+ +L   ISP  SP+  R K +KDL ++V N + +DK  E L Y  KDLL  D P + RH+ L+F   LV GQYS+L  L+R  FF ++KE++      D    ++LL  LT  GKDI++ E+ +GPF+ + +P +T        +FL ++ N   FN+ Y+D D++  L+  I  LC  +N       CLE L++I+CYS +  + L  FI  LC  VN+E Y + +W I R L+GTH+GHSALY +  ++Q T  + DV L+RGA+F++   LWG ++I  L+ +  ++LP+F  ALKC H +V++EVI  +++LV  Y  EL       I+++IE ++       +     +S +LH  I  +E L ++  Y+G  Q+ + +++ C+D RPE SV++LI +R +SI      W    + L+ERYYK E R NIR+K LDV+ ++ + N   +EEELI+RII+P+   V M+ +  IR     +L  LC  C T + L+LL+IL  ++ +                                                  LEQ  +  ++  D+           + +  +  YHLG  ++       FSPY++     E    E + +    ++  P +P+ L     + +  +SL  AC  VI C++ E DW VL LVL  +P V+QNR LI  R+   I+ F   L S  +DK+   P  L N P KF+ S+F  H+FPVLA+LASY+  L P  Q  LI+ LE+GL +K C   C+ +LTT  LEM+ +   L+ EVLLN SKISAT  I+ PILEFLST+ RLP+VF+ F    Y++VFAI LP+TNPFK+D YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N+ VPFEE        N  N+DS  R RS+SL    S  RR    +  +    + A+D+        + FH ELTETC+D++ARY +   S + +RL  +DF+LK   S TWL+G  LITV+TS C++ AMRNG+CD C+  C + G  S +               +  K     R  S  Y  +  +  +N  +++          + +   LE+M+    +  +++++L          C+CWC GWAEIY+RRP+GD+SW+ R+QN+     +     + ++ AL+  + +LK+  F  + S +     +DK N+D +  D    K + S        +P S + PS     +     S  D E  +   ++PV+++ SSP
Sbjct:    4 MSSKDKDNKTLHDKLKQFFRINKGN-YKSREDFTLTHDLEKEISPE-SPVHHRTKAIKDLCDAVLNQQWEDKAAERLWYLVKDLLEKDIPREHRHVTLNFLRCLVQGQYSKLSSLMRVKFFMVVKEHNIPE---DIGPRLELLQSLTENGKDILHLEERMGPFLLDWMPIVTAGDGKRGAEFLSLLVNVIKFNSAYIDEDIISGLVQYICHLCYYSNSSEVVSGCLEALDAIVCYSNLQSDSLQTFIIALCGSVNVETYCQISWKIMRNLLGTHMGHSALYTMCRLLQDTNFQRDVRLLRGAVFYVNMGLWGTHKIPKLECTPTSVLPSFYQALKCNHPVVMYEVILSIQRLVNKYGTELWDPTWSIILDIIEEVISHTETSNQPATRQVSVNLHETINSIENLLDINHYNGCIQRFYDLVERCSDARPEGSVLKLIEYRARSIGPTHYHWQFKLANLMERYYKIETRTNIRMKVLDVLTNVVQINRSRYEEELIERIIVPYFQHVDMDFDITIRNGVAHLLIDLCLECDTKRCLELLDILEKVINKPFTSDIPVTKDVDIKDIKTAVVGVIKILISKIYYLPSSHAIRAYKVLVNYLEQHYKEPTVFYDIPTIRYLIFECFLKIRANTLYHLGFPDTQNLSVIKFSPYLVL----EHTTIERINSGGGGNSPPPVNPTPLQHLSCQ-ITYMSLALACKAVISCIKLEKDWKVLQLVLKELPQVMQNRALILSRHTNDIDYFANALCSMVSDKSLRLPESLYNVPPKFTLSEFRVHVFPVLASLASYHAHLEPNLQQRLIKCLEVGLTAK-CASQCVTSLTTCILEMRDAMNKLLSEVLLNLSKISATVHIAIPILEFLSTLTRLPKVFASFIGDQYMSVFAILLPYTNPFKYDHYTVSLAHHVIAVWFLKCRLPFRRDFVKFITTGLKANVIVPFEEGHLMKSDFNFINEDSSNRKRSSSLTEQGSRGRRERPIMTNRMIGDNKASDLKPPIDEALMTFHVELTETCIDLMARYTFSTYSARPKRLPAADFLLKEAQSMTWLLGNKLITVTTSGCSNKAMRNGLCDNCWVVC-KSGPQSPEHSKALQSNLRRASSIETAKEDKLSRQFSGGYGSSTVNTAANSPTEELKKTSEDLDSIKKDHPLEKMDKDQAESSKLEQILNSEKQEEHVLCACWCQGWAEIYVRRPTGDMSWIMRIQNSMQFETNI-DFPVYDIMALYRPNQNLKQQEFTSEYSGDETEDIADK-NMDQIKNDHNNMKSMISSVSSGPIAIPGSPARPSPSKQSSRDSLESLEDGEDDLRRSRNPVRRSNSSP 1304          
BLAST of EMLSAG00000002599 vs. nr
Match: gi|571577991|ref|XP_006572242.1| (PREDICTED: tuberin isoform X3 [Apis mellifera])

HSP 1 Score: 774.622 bits (1999), Expect = 0.000e+0
Identity = 467/1315 (35.51%), Postives = 706/1315 (53.69%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLG-LVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP-QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRS-------------------------------------------------LEQEAERDSLLKDL----------LIVVAEDESYHLGLEESD--GSXXFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAK---KDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKD---------------DITKNSDRRRHMSDFYSGTNKSQLSNLRSDK---------ELHRHTSLERME-SSGDDYRVKKVLPET-------CSCWCSGWAEIYIRRPSGDVSWMCRVQNT------------QLLSAHTPSTNLRELTALFDTSLKKDTFNFDVSTESDKINVD-NLTEDEYKKLSSE--FVPSSVSSPSAETDKTVSGNRS-HDEEMIVMSPKSPVKKTKSSP 1186
            MS   K  K   +KLKQ+F+  K N Y   E+  L+ +L   ISP  SP+  R K +KDL ++V N + +DK  E L Y  KDLL  D P + RH+  +F   LV GQYS+L  L+R  FF ++KE++      D    ++LL  LT  GKDI++ E+ +GPF+ + +P +T        +FL ++ N   FN+ Y+D D++  L+  I  LC  +N       CLE L++I+CYS +  + L  FI  LC  VN+E Y + +W I R L+GTH+GHSALY +  ++Q T  + DV L+RGA+F++   LWG ++I  L+ +  ++LP+F  ALKC H +V++EVI  +++LV  Y  EL       I+++IE ++       +     +S +LH  I  +E L ++  Y+G  Q+ + +++ C+D RPE SV++LI +R +SI      W    + L+ERYYK E R NIR+K LDV+ ++ + N   +EEELI+RII+P+   V M+ +  IR     +L  LC  C T + L+LL+IL  ++ +                                                  LEQ  +  ++  D+           + +  +  YHLG  ++       FSPY+I     E    E + +    ++  P +P+ L     + +  +SL  AC  VI C++ E DW VL LVL  +P V+QNR LI  R+   I+ F   L S  +DK+   P  L N P KF+ S+F  H+FPVLA+LASY+  L P  Q  LI+ LE+GL +K C   C+ +LTT  LEM+ +   L+ EVLLN SKISAT  I+ PILEFLST+ RLP+VF+ F    Y++VFAI LP+TNPFK+D YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N+ VPFEE        N  N+DS  R RS+SL    S  RR    +  +    +  +D+        + FH ELTETC+D++ARY +   S + +RL ++DF+LK G S TWL+G  LITV+TS C++ AMRNG+CD C+  C + G  S +               +  K     R  S  Y  +  +  +N  +++          + +  SLE+++    +  +++++L          C+CWC GWAEIY+RRP+GD+SW+ R+QN+             +++ + P+ +L++    F +    D          D+I  D N  +     +SS    +P S + PS     +     S  D E  +   ++PV+++ SSP
Sbjct:    4 MSSKDKDNKTLHDKLKQFFRINKGN-YKSREDFTLTHDLEKEISPE-SPVHHRTKAIKDLCDAVLNQQWEDKAAERLWYLVKDLLEKDVPREHRHVTFNFLRCLVQGQYSKLSSLMRVKFFMVVKEHNIPE---DIGPRLELLQSLTENGKDILHLEERMGPFLLDWMPIVTAGDGKRGAEFLLLLVNVIKFNSAYIDEDIISGLVQYICHLCYYSNSSEVVSGCLEALDAIVCYSNLQSDSLQTFIIALCGSVNVETYCQISWKIMRNLLGTHMGHSALYTMCRLLQDTNFQRDVRLLRGAVFYVNMGLWGTHKIPKLECTPTSVLPSFYQALKCNHPVVMYEVILSIQRLVNKYGTELWDPTWSIILDIIEEVISHTETSNQPATRQVSVNLHETINSIENLLDINHYNGCIQRFYDLVERCSDARPESSVLKLIEYRARSIGPTHYHWQFKLANLMERYYKIETRTNIRMKVLDVLTNVVQINRSRYEEELIERIIVPYFQHVDMDFDITIRNGVAHLLIDLCLECDTKRCLELLDILEKVINKPFTSDIPITKDVDIKDIKTAVVGVIKILISKIYYLPSSHAIRAYKVLVNYLEQHYKEPTVFYDIPTIRYLIFECFLKIRANTLYHLGFPDTQNLSVIKFSPYLIL----EHTTTERINSGGGGNSPPPVNPAPLQHLSCQ-ITYMSLALACKAVISCIKLEKDWKVLQLVLKELPQVMQNRALILSRHTNDIDYFANALCSMVSDKSLRLPESLYNVPPKFTLSEFRVHVFPVLASLASYHAHLEPNLQQRLIKCLEVGLTAK-CASQCVTSLTTCILEMRDAMNKLLSEVLLNLSKISATVHIAIPILEFLSTLTRLPKVFASFIGDQYMSVFAILLPYTNPFKYDHYTVSLAHHVIAVWFLKCRLPFRRDFVRFITTGLKANVIVPFEEGHLMKSDFNFINEDSSNRKRSSSLTEQGSRGRRERPIMTNRMIGDNKVSDLKPPIDEALMTFHVELTETCIDLMARYTFSTYSARPKRLPSADFLLKEGQSMTWLLGNKLITVTTSGCSNKAMRNGLCDNCWVVC-KSGPQSPEHSKALQSNLRRASSIETAKEDKLSRQFSGGYGNSTVNTAANSPTEELKKTSEDLDSIKKDHSLEKIDKDQAESSKLEQILNSEKQEEHVLCACWCQGWAEIYVRRPTGDMSWIMRIQNSMQFETNIDFPVYDIMALYRPNQDLKQKQQEFTSEYSGDETEDIADKNMDQIKNDHNNMKSMISSVSSGPIAIPGSPARPSPSRQSSRDSLESLEDGEDDLRRSRNPVRRSNSSP 1306          
BLAST of EMLSAG00000002599 vs. nr
Match: gi|808146060|ref|XP_003402526.2| (PREDICTED: tuberin [Bombus terrestris])

HSP 1 Score: 773.081 bits (1995), Expect = 0.000e+0
Identity = 450/1201 (37.47%), Postives = 670/1201 (55.79%), Query Frame = 0
Query:    1 MSKSSKAEKPFSEKLKQWFKGTKSNLYPHGEEIRLSGELVDSISPSISPLQERLKILKDLKESVRNGRLQDKGVEVLLYKTKDLLRNDSP-DVRHIVLSFYESLVYGQYSRLG-LVRSAFFNIIKENHSKRGSADFTYHIDLLYVLTGKGKDIVYFEDELGPFIKETIPFITP-QLPCTLKFLEMITNCFIFNATYLDSDVVLALITLISSLCTSTNDKNETKSCLEVLNSIICYSYIPKNGLLLFISILCRVVNLEAYGKDAWDITRRLMGTHLGHSALYCLSMIVQTTENRSDVALIRGAIFFIGFSLWGHNRIKSLDYSAVTILPTFVHALKCKHHLVVFEVIQQVKQLVTFYDKELHAAACDAIVELIELLLIEVRRLEKKLQDNISSHLHAVITILEGLSEMKRYSGSKQKLFGMLDLCADHRPEESVIRLITFRQKSIHAAKSKWLNIFSELVERYYKKEKRVNIRIKTLDVILDIFKSNHYSFEEELIDRIIIPFLGSVHMEPESRIRRRAIEVLTTLCSLCQTNKNLDLLNILGCIMKRS-------------------------------------------------LEQEAERDSLLKDL----------LIVVAEDESYHLGLEESDGSX--XFSPYIICRSPEEEEEAELLWNRQQESASRPSSPSCLSGTRQKSVLCVSLNKACLLVIECLERETDWNVLCLVLTRVPTVLQNRPLI-CRYGKSINKFITPLVSF-TDKNSNFPLHLTNTPSKFSKSDFHNHIFPVLAALASYNEFLVPASQSSLIRALELGLLSKECNRMCIVALTTSALEMKRSTRNLIPEVLLNFSKISATKFISTPILEFLSTMVRLPEVFSCFTDTNYITVFAIALPFTNPFKFDPYTVSLAHHVIVMWFLKCRLSYRQNFVQFIITSLNSNIFVPFEE--------NDKNKDSMGRMRSTSL----SAARRPNVALAAK---KDVAADVPKXTQIHPLAFHQELTETCLDILARYMYGNVSVKTRRLKTSDFILKNGHSATWLIGTMLITVSTSCCTHIAMRNGVCDRCYFYCIRDGTLSKDDI-TKNSDRRR----------HMSDFYSGTNKSQLSNLRS------------DKELHR--HTSLERMESSGDDYRVKKVLPET-------CSCWCSGWAEIYIRRPSGDVSWMCRVQNT 1088
            M+   K  K   +KLKQ+F+  K N Y   E++ L+ +L   ISP  SP+  R K++KDL ++V N + +DK  E L Y  +DLL  D P + RH+ L+F   LV GQYS+L  L+R  FF ++KE++      D    +DLL  LT  GKDI++ E+ +GPF+ + +P +T        +FL ++ N   FN+ Y+D D++  L+  I  LC  +N       CLE L++I+CYS +  + L  FI  LC  VN+E Y + +W I R L+GTH+GHSALY +  ++Q T  + DV L+RGA+F++   LWG +RI  L+ +  ++LP+F  ALKC H +V++EV   +++LV  Y  EL       I+++IE ++       +     +S  LH  I  +E L +   Y+G  Q+ + +++ C+D RPE S+++LI +R ++I A    W +  + L+ERYYK E R N+R+K LDV+ ++ + N   +E+ELI+RII+P+   V M+ +  IR     +L  LC  C T + L+LL+IL  ++ +                                                  LEQ  +  ++  D+           + +  +  YHLG  ++  S    F+PY++     E    E + +    S+  P SP+ L     + +  +SL  AC  VI C++ E DW VL LVL  +P V+QNR LI  R+   I+ F T L S  +DK+   P  L N P KF+ S+F  H+FPVLA+LASY+  L P  Q  LI+ LE+GL +K C   C+ +LTT  LEM+ +   L+ EVLLN SKISAT  I+ PILEFLST+ RLP+VF+ F    Y++VFAI LP+TNPFK+D YTVSLAHHVI +WFLKCRL +R++FV+FI T L +N+ VPFEE        N  N+DS  R RS+SL    S  RR    +A +    +  +D+        + FH ELTETC+D++ARY +   S + +RL  +DF+LK G S TWL+G  LITV+TS C++ AMRNG+CD C+  C + G  S + + T  S  RR           +S   SG + S  +N  +            D +L +  H+S +  +   +  +++++L          C+CWC GWAEIY+RRP+GD+SW+ R+QN+
Sbjct:    4 MNSKDKDNKTLHDKLKQFFRINKGN-YKSREDVTLTHDLEKEISPE-SPVHHRTKVIKDLCDAVLNQQWEDKAAERLWYLVQDLLEKDVPREHRHVTLNFLRCLVQGQYSKLSSLMRVKFFMVVKEHNIPE---DIGPRLDLLQSLTENGKDILHLEERMGPFLLDWMPVVTAGDGKRGAEFLSLLVNVIKFNSAYIDEDIISGLVQYICHLCYYSNSSEVVSGCLEALDAIVCYSNLQSDSLQTFIIALCGSVNVETYCQISWKIMRNLLGTHMGHSALYTMCRLLQDTNFQRDVRLLRGAVFYVNMGLWGTHRIPKLECTPTSVLPSFYQALKCNHPIVMYEVTLSIQRLVNKYGAELWDPTWSIILDIIEEVISHTETSNQPATRQVSVSLHETINSIENLLDSNYYNGCIQRFYDLVERCSDARPEGSILKLIEYRARTIGATHHLWQSRLANLMERYYKIETRTNVRMKVLDVLTNVVQVNRSRYEDELIERIIVPYFQHVDMDSDITIRNGVAHLLVDLCLECDTKRCLELLDILEKVINKPFTSDTPVTKDVDIKDVKTAVVGVIKILTSKIYYLPSSHAIRAYKVLVNHLEQHYKEPTVFHDISTIRYLIFECFLKIRANTLYHLGFPDAQNSSVIKFTPYLVL----EHATTERINSGGGGSSPPPVSPAPLQHLSCQ-ITHMSLAHACKAVISCIKSEKDWKVLQLVLKELPQVMQNRALILSRHTNDIDFFATALCSMVSDKSLRLPESLYNVPPKFTLSEFRVHVFPVLASLASYHAHLEPNLQQRLIKCLEVGLTAK-CASQCVTSLTTCILEMRDAMNKLLSEVLLNLSKISATVHIAIPILEFLSTLTRLPKVFASFIGDQYMSVFAILLPYTNPFKYDHYTVSLAHHVIAVWFLKCRLPFRRDFVRFITTGLKANVIVPFEEGHLMKSDFNFINEDSSNRKRSSSLTEQGSRGRRERPIMANRMIGDNKVSDLKPPIDEALMTFHVELTETCIDLMARYTFSTYSARPKRLAAADFLLKEGQSMTWLLGNKLITVTTSGCSNKAMRNGLCDNCWVVC-KPGPQSPEHLKTSQSTLRRASSTETAKEDKLSRQSSGGHGSSTANTAANSPTEESKKTSEDSDLTKKDHSSEKMEKDQAESSKLEQILNSEKQEEHVLCACWCQGWAEIYVRRPTGDMSWIMRIQNS 1192          

HSP 2 Score: 273.092 bits (697), Expect = 2.556e-70
Identity = 151/341 (44.28%), Postives = 207/341 (60.70%), Query Frame = 0
Query: 1231 RSHTISVSSPRNRVLNKPLQPTTGIVGRPGIGGIGSGEKNDKVSGISPQFVFLMLYQSSTFGNAASLEKPLLLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPELKEPLGHV-------MSLSILVRQWALHCNLAAQIQKSLSNKM--PYSSNWLZXLRAIKRLKEKLQKENEMKEEEDREQGSGRPQ----LNDFTEF 1558
            R HTISV SP        ++ + G       G     ++  K S I+P FVFL LY ++ FG+ +  EKPLL+P     + ++ NLDRI  +ETHKIGVLY+GP Q ++E  IL N  GS RYT+FL  LG L+ ++++D+S  F+GGLD    +GNFAY+W DDV Q  FHVATLMP   +DP+   KK+HIGNNY  +VYN+S    E Y + TV+G+F  AC+VI PLD G+N+V V  K EL + + H         +L+IL RQ ALH NLA+ +  SL      PY+SNWL+ LR IKRL+ ++ +E     +   +  S R      ++DFTE+
Sbjct: 1500 RGHTISVMSP--------VKKSRGEWDNIRRGNSPRVKETPKTS-INPSFVFLQLYHTAHFGSPS--EKPLLVPQTTAVQRAVTNLDRIQPYETHKIGVLYVGPGQASNETEILANQHGSLRYTEFLQRLGTLVRLKDVDESV-FLGGLDRNGENGNFAYIWQDDVTQVAFHVATLMPTKLSDPKCTSKKQHIGNNYVTVVYNES---GEPYNIQTVKGQFNYACVVIQPLDHGTNQVTVQVKEELVKHIRHSEPKIISDQNLAILSRQLALHANLASMVSSSLEQNSHNPYASNWLERLRHIKRLRNRVLQETNNNPDRTTDDLSPRSNKRIYMDDFTEY 1825          
BLAST of EMLSAG00000002599 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold1916_size24942-snap-gene-0.4 (protein:Tk07384 transcript:maker-scaffold1916_size24942-snap-gene-0.4-mRNA-1 annotation:"250 kda substrate of akt")

HSP 1 Score: 94.7449 bits (234), Expect = 1.928e-19
Identity = 60/175 (34.29%), Postives = 90/175 (51.43%), Query Frame = 0
Query: 1302 LLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPEL 1476
            L+  N+     +KNLD     E HKI V+++G  Q  D+  IL N SGS  Y +F+ GL   + +   +  T F+GGL   +  G  A  +    ++ +FHVAT MP+   +    +K RHIGN+   IV+++   H   Y+ G +  +F    IVI PL     R+ V  KPE+
Sbjct: 1600 LMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQ-EDKQSILSNASGSQAYEEFVSGLAWEVEL---ETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESML-QKTRHIGNDEIHIVWSE---HWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEV 1766          
BLAST of EMLSAG00000002599 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold198_size266703-processed-gene-0.0 (protein:Tk12339 transcript:snap_masked-scaffold198_size266703-processed-gene-0.0-mRNA-1 annotation:"250 kda substrate of akt")

HSP 1 Score: 93.5893 bits (231), Expect = 3.767e-19
Identity = 60/175 (34.29%), Postives = 90/175 (51.43%), Query Frame = 0
Query: 1302 LLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPEL 1476
            L+  N+     +KNLD     E HKI V+++G  Q  D+  IL N SGS  Y +F+ GL   + +   +  T F+GGL   +  G  A  +    ++ +FHVAT MP+   +    +K RHIGN+   IV+++   H   Y+ G +  +F    IVI PL     R+ V  KPE+
Sbjct:  909 LMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQ-EDKQSILSNASGSQAYEEFVSGLAWEVEL---ETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESML-QKTRHIGNDEIHIVWSE---HWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEV 1075          
BLAST of EMLSAG00000002599 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold90_size386344-snap-gene-0.18 (protein:Tk12038 transcript:maker-scaffold90_size386344-snap-gene-0.18-mRNA-1 annotation:"hypothetical protein DAPPUDRAFT_319317")

HSP 1 Score: 75.0998 bits (183), Expect = 1.228e-13
Identity = 44/136 (32.35%), Postives = 72/136 (52.94%), Query Frame = 0
Query: 1326 KIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEE---GDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVI 1458
            K+GV+Y+  NQ  +E  IL N   S  + +FL  LG  + +R  D+   + GGLD      G  +    W    ++ +FHV+TL+P   NDPQ  ++KRHIGN+   +V+ ++ N    +    ++  F+   IV+
Sbjct:    2 KVGVIYVKENQYTEE-EILANNDHSDNFDEFLTILGEKVRLRGFDR---YKGGLDTVHDLTGHHSVCTHWRS--IEVMFHVSTLLPFEQNDPQKLQRKRHIGNDIVCVVFLEADN--TQFSPSCIKSHFLHTFIVV 129          
BLAST of EMLSAG00000002599 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold33_size549341-snap-gene-3.3 (protein:Tk11541 transcript:maker-scaffold33_size549341-snap-gene-3.3-mRNA-1 annotation:"hypothetical protein TcasGA2_TC006305")

HSP 1 Score: 68.5514 bits (166), Expect = 1.305e-11
Identity = 45/154 (29.22%), Postives = 78/154 (50.65%), Query Frame = 0
Query: 1313 IKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGD-GNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGS 1465
            I N D      + K G++Y    Q+ +E  +  N   S    +F+D LG  I++ +      + GGLD + G  G+ +        + +FHV+T++P  +ND Q  ++KRHIGN+  AI++ D    S  +    +   F+ A I++ P+D G+
Sbjct:  411 IVNYDEHVLDNSFKFGLIYQKVGQITEEA-LFGNRQPSQAMEEFMDILGQKISLSD---HKGYRGGLDTQYGQTGDESLYEKFHGREIMFHVSTMLPYTENDSQQLQRKRHIGNDIVAIIFQDG---STPFTPDMITSHFLHAYILVQPIDPGT 557          
BLAST of EMLSAG00000002599 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold33_size549341-snap-gene-4.30 (protein:Tk11552 transcript:maker-scaffold33_size549341-snap-gene-4.30-mRNA-1 annotation:"hypothetical protein TcasGA2_TC006305")

HSP 1 Score: 67.0106 bits (162), Expect = 4.641e-11
Identity = 42/140 (30.00%), Postives = 74/140 (52.86%), Query Frame = 0
Query: 1326 KIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVM---QXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLD 1462
            K G++Y    Q+ +E  +  N S SS + +F+  +G  I++    +   + GGLD + G      ++  DV    + +FHV+T++P  +ND Q  ++KRHIGN+  AIV+ D       +    +   F+ A I++ P+D
Sbjct:  574 KFGLIYQKGGQITEE-SMFNNQSHSSAFDEFISLMGQRISLA---QHKGYRGGLDTQYGQTGDESLY--DVFHGREIMFHVSTMLPFTENDVQQVQRKRHIGNDIVAIVFQDGNT---PFTPDMITSHFLHAYILVQPID 704          
BLAST of EMLSAG00000002599 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold506_size152672-snap-gene-0.30 (protein:Tk07585 transcript:maker-scaffold506_size152672-snap-gene-0.30-mRNA-1 annotation:"gtpase-activating rap ran-gap domain-like protein 3-like")

HSP 1 Score: 58.5362 bits (140), Expect = 1.318e-8
Identity = 34/111 (30.63%), Postives = 61/111 (54.95%), Query Frame = 0
Query: 1326 KIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGD-GNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDS 1435
            K GV+Y    Q+ D+  +  N  G   +  FL+ LG  I +++  K   + GGLD ++   G+ +     +  + +FHV+TL+P  ++D Q  ++KRHIGN+   +++ D 
Sbjct:   13 KFGVIYAKAGQILDD-ELFSNAEGGEDFDRFLNLLGQRIRLKDWKK---YKGGLDVKKDQTGHNSVFTEFEEHEIMFHVSTLLPYSEDDKQQIERKRHIGNDIVNVIFYDG 119          
The following BLAST results are available for this feature:
BLAST of EMLSAG00000002599 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO)
Total hits: 25
Match NameE-valueIdentityDescription
-2.568e-14330.85symbol:TSC2 "Tuberin" species:9606 "Homo sapiens" ... [more]
-5.216e-14330.82symbol:TSC2 "Tuberin" species:9606 "Homo sapiens" ... [more]
-2.927e-14230.55symbol:TSC2 "Uncharacterized protein" species:9615... [more]
-6.505e-14029.19symbol:TSC2 "Uncharacterized protein" species:9031... [more]
-8.759e-14030.03symbol:TSC2 "Uncharacterized protein" species:9913... [more]
-1.518e-13829.78symbol:Tsc2 "tuberous sclerosis 2" species:10090 "... [more]
-2.068e-13829.55symbol:Tsc2 "Tuberin" species:10116 "Rattus norveg... [more]
-2.068e-13829.55symbol:Tsc2 "tuberous sclerosis 2" species:10116 "... [more]
-3.148e-13829.93symbol:TSC2 "Tuberin" species:9606 "Homo sapiens" ... [more]
-2.438e-13729.64symbol:TSC2 "Uncharacterized protein" species:9615... [more]

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BLAST of EMLSAG00000002599 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA)
Total hits: 25
Match NameE-valueIdentityDescription
gi|592925097|gb|GAXK01033318.1|1.623e-12439.60TSA: Calanus finmarchicus comp83597_c1_seq1 transc... [more]
gi|592925091|gb|GAXK01033324.1|1.933e-12439.60TSA: Calanus finmarchicus comp83597_c9_seq1 transc... [more]
gi|592925096|gb|GAXK01033319.1|4.580e-8249.16TSA: Calanus finmarchicus comp83597_c1_seq2 transc... [more]
gi|592925093|gb|GAXK01033322.1|1.065e-2842.68TSA: Calanus finmarchicus comp83597_c1_seq5 transc... [more]
gi|592925092|gb|GAXK01033323.1|5.958e-2641.61TSA: Calanus finmarchicus comp83597_c1_seq6 transc... [more]
gi|592925088|gb|GAXK01033327.1|4.546e-2557.95TSA: Calanus finmarchicus comp83597_c9_seq4 transc... [more]
gi|592925090|gb|GAXK01033325.1|1.425e-2336.41TSA: Calanus finmarchicus comp83597_c9_seq2 transc... [more]
gi|592925094|gb|GAXK01033321.1|1.425e-2336.41TSA: Calanus finmarchicus comp83597_c1_seq4 transc... [more]
gi|592925095|gb|GAXK01033320.1|1.425e-2336.41TSA: Calanus finmarchicus comp83597_c1_seq3 transc... [more]
gi|592925089|gb|GAXK01033326.1|1.914e-2035.15TSA: Calanus finmarchicus comp83597_c9_seq3 transc... [more]

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BLAST of EMLSAG00000002599 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self)
Total hits: 4
Match NameE-valueIdentityDescription
EMLSAP000000025990.000e+0100.00pep:novel supercontig:LSalAtl2s:LSalAtl2s151:40339... [more]
EMLSAP000000049743.947e-1729.66pep:novel supercontig:LSalAtl2s:LSalAtl2s260:14910... [more]
EMLSAP000000099515.589e-1432.47pep:novel supercontig:LSalAtl2s:LSalAtl2s64:61995:... [more]
EMLSAP000000099421.260e-1328.50pep:novel supercontig:LSalAtl2s:LSalAtl2s64:423747... [more]
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BLAST of EMLSAG00000002599 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt)
Total hits: 21
Match NameE-valueIdentityDescription
gi|9297076|sp|Q61037.1|TSC2_MOUSE1.635e-13929.78RecName: Full=Tuberin; AltName: Full=Tuberous scle... [more]
gi|1717800|sp|P49816.1|TSC2_RAT2.218e-13929.55RecName: Full=Tuberin; AltName: Full=Tuberous scle... [more]
gi|269849475|sp|P49815.2|TSC2_HUMAN3.390e-13929.93RecName: Full=Tuberin; AltName: Full=Tuberous scle... [more]
gi|62287828|sp|Q9UUG9.1|TSC2_SCHPO7.094e-3032.86RecName: Full=Tuberous sclerosis 2 protein homolog[more]
gi|284022067|sp|P86411.1|RGPA2_RAT9.851e-2137.29RecName: Full=Ral GTPase-activating protein subuni... [more]
gi|147742932|sp|Q2PPJ7.2|RGPA2_HUMAN1.090e-2037.29RecName: Full=Ral GTPase-activating protein subuni... [more]
gi|147742933|sp|A3KGS3.2|RGPA2_MOUSE1.227e-2037.29RecName: Full=Ral GTPase-activating protein subuni... [more]
gi|51315849|sp|Q6GYP7.1|RGPA1_MOUSE2.069e-2032.44RecName: Full=Ral GTPase-activating protein subuni... [more]
gi|703556455|sp|O55007.2|RGPA1_RAT5.837e-2033.98RecName: Full=Ral GTPase-activating protein subuni... [more]
gi|51315850|sp|Q6GYQ0.1|RGPA1_HUMAN1.838e-1932.00RecName: Full=Ral GTPase-activating protein subuni... [more]

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BLAST of EMLSAG00000002599 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods)
Total hits: 25
Match NameE-valueIdentityDescription
XP_006572241.16.499e-7435.67PREDICTED: tuberin isoform X1 [Apis mellifera][more]
XP_016772912.13.343e-6335.67PREDICTED: tuberin isoform X2 [Apis mellifera][more]
XP_006572242.10.000e+035.51PREDICTED: tuberin isoform X3 [Apis mellifera][more]
EEB16407.14.347e-7534.71tuberin, putative [Pediculus humanus corporis][more]
EFX68537.11.854e-6537.70putative tuberous sclerosis 2 isoform 3-like prote... [more]
gb|EFA09042.2|5.159e-15329.23Tuberin-like Protein [Tribolium castaneum][more]
EAA05274.58.071e-15031.39AGAP011123-PA, partial [Anopheles gambiae str. PES... [more]
gb|KFM71943.1|9.486e-10333.69Tuberin, partial [Stegodyphus mimosarum][more]
AAF49066.13.606e-6629.69gigas, isoform A [Drosophila melanogaster][more]
AGB94782.13.786e-6629.69gigas, isoform B [Drosophila melanogaster][more]

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BLAST of EMLSAG00000002599 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017))
Total hits: 25
Match NameE-valueIdentityDescription
gi|939636378|ref|XP_014294198.1|0.000e+034.15PREDICTED: tuberin [Halyomorpha halys][more]
gi|1035616060|ref|XP_016915834.1|3.180e-7136.14PREDICTED: tuberin isoform X1 [Apis cerana][more]
gi|380029021|ref|XP_003698181.1|1.974e-7135.58PREDICTED: LOW QUALITY PROTEIN: tuberin [Apis flor... [more]
gi|572315473|ref|XP_006623386.1|6.001e-7135.89PREDICTED: tuberin-like isoform X1 [Apis dorsata][more]
gi|571577989|ref|XP_006572241.1|3.180e-7135.67PREDICTED: tuberin isoform X1 [Apis mellifera][more]
gi|1031995841|ref|XP_016772912.1|1.636e-6035.67PREDICTED: tuberin isoform X2 [Apis mellifera][more]
gi|815895494|ref|XP_012243202.1|2.715e-7035.46PREDICTED: tuberin [Bombus impatiens][more]
gi|572315475|ref|XP_006623387.1|0.000e+035.74PREDICTED: tuberin-like isoform X2 [Apis dorsata][more]
gi|571577991|ref|XP_006572242.1|0.000e+035.51PREDICTED: tuberin isoform X3 [Apis mellifera][more]
gi|808146060|ref|XP_003402526.2|2.556e-7037.47PREDICTED: tuberin [Bombus terrestris][more]

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BLAST of EMLSAG00000002599 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins)
Total hits: 6
Match NameE-valueIdentityDescription
maker-scaffold1916_size24942-snap-gene-0.41.928e-1934.29protein:Tk07384 transcript:maker-scaffold1916_size... [more]
snap_masked-scaffold198_size266703-processed-gene-0.03.767e-1934.29protein:Tk12339 transcript:snap_masked-scaffold198... [more]
maker-scaffold90_size386344-snap-gene-0.181.228e-1332.35protein:Tk12038 transcript:maker-scaffold90_size38... [more]
maker-scaffold33_size549341-snap-gene-3.31.305e-1129.22protein:Tk11541 transcript:maker-scaffold33_size54... [more]
maker-scaffold33_size549341-snap-gene-4.304.641e-1130.00protein:Tk11552 transcript:maker-scaffold33_size54... [more]
maker-scaffold506_size152672-snap-gene-0.301.318e-830.63protein:Tk07585 transcript:maker-scaffold506_size1... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
LSalAtl2s151supercontigLSalAtl2s151:403390..412004 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
ensembl2013-09-26 .965016
Blast vs. GO2014-04-02
TblastN vs C. finmarchicus TSA2014-05-09
Blastp vs. self2014-05-10
Blastp vs. SwissProt2017-02-10
Blastp vs. Selected Arthropods2017-02-20
Blastp vs. NR (2/2017)2017-02-20
Blastp vs. Tigriopus kingsejongensis proteins2018-04-18
Properties
Property NameValue
Logic nameensemblgenomes
Descriptionmaker-LSalAtl2s151-augustus-gene-4.14
Biotypeprotein_coding
EvidenceIEA
NoteTuberin
Cross References
External references for this gene
DatabaseAccession
Ensembl Metazoa (gene)EMLSAG00000002599 (primary cross-reference)
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
EMLSAT00000002599EMLSAT00000002599-698446Lepeophtheirus salmonismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at LSalAtl2s151:403390..412004-

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>EMLSAG00000002599-685365 ID=EMLSAG00000002599-685365|Name=EMLSAG00000002599|organism=Lepeophtheirus salmonis|type=gene|length=8615bp|location=Sequence derived from alignment at LSalAtl2s151:403390..412004- (Lepeophtheirus salmonis)
ATGTCCAAGTCCAGCAAAGCCGAGAAACCTTTCTCAGAGAAACTTAAACA ATGGTTCAAGTAAGAATATGCTTGGATTGATATTATTATTTAAGTTCCAT CATCATCATCATTTTCTTTTGACTTTAAAGGGGCACGAAATCCAATTTAT ATCCACATGGAGAGGAAATCCGCCTTAGTGGAGAGCTGGTCGACTCTATT TCCCCTTCCATATCCCCACTCCAGGAACGTTTAAAGATCCTTAAAGACCT GAAGGAAAGCGTGAGGAATGGGCGACTTCAAGACAAAGGAGTTGAGGTCC TTCTCTACAAAACAAAGGATTTACTGCGAAATGATTCCCCTGACGTACGT CATATTGTGCTATCCTTTTATGAATCCCTTGTCTATGGGCAATACTCTCG TTTGGGCCTAGTTCGATCCGCATTCTTCAATATCATCAAGGAAAATCATT CAAAGAGAGGGAGTGCGGACTTTACATATCATATCGATCTATTGTACGTA CTAACAGGGAAAGGGAAGGACATTGTGTATTTCGAAGATGAGTTGGGCCC ATTTATCAAGGAAACGATTCCCTTTATTACGCCGCAATTACCCTGTACTC TGAAATTCTTGGAGATGATTACAAATTGTTTTATATTCAACGCAACCTAT CTGGATTCGGATGTTGTTTTAGCACTTATTACATTAATAAGTAGTCTTTG CACGTCTACGAATGATAAAAATGAAACCAAATCCTGTCTTGAAGTAAGGA CCACCCTTTTAAAATCTCCTTTATCATAGTCAACACTTTTGAATTTCAGG TTTTGAATTCCATCATTTGCTACAGTTACATCCCCAAAAATGGACTCTTA CTCTTCATTAGCATACTTTGTCGAGTTGTAAATTTAGAAGCGTATGGAAA GGATGCTTGGGACATTACTCGTCGTTTAATGGGTACACATCTGGGTCACT CAGCCTTGTATTGTTTGAGCATGATTGTTCAAACTACTGAGAATCGTAGT GATGTTGCTCTTATACGTGGAGCAATATTCTTTATTGGATTTAGTTTGTG GGGACACAATCGAATCAAATCTTTGGATTATTCCGCTGTCACCATCTTAC CTACCTTTGTACATGCTCTTAAATGTAAGCATCATTTGGTTGTTTTTGAA GTGATTCAGCAGGTAATATATATATTATAAGTAATTGACTTGAACATGAT CCAACAATAATCATTTTCATGGCAATTTACAGGTCAAACAATTAGTCACG TTCTATGATAAAGAACTTCATGCCGCCGCTTGTGATGCCATTGTCGAACT CATTGAACTGCTTCTCATTGAAGTTCGTCGATTGGAAAAAAAACTTCAGG ACAACATATCCTCACATTTGCATGCAGTTATCACCATATTAGAGGGTCTA TCAGAAATGAAAAGGTATGATGCCTTTAAAGAAGAAAATATATAACATTT TAGGGATTTTTATTTAATCATATATTGCTTATAGATATAGTGGAAGCAAA CAAAAGTTGTTTGGAATGCTGGACTTGTGTGCTGATCACCGCCCTGAAGA ATCTGTAATCAGGTTAATAACTTTCCGTCAGAAATCAATACATGCAGCAA AGTCTAAATGGCTTAACATCTTTTCTGAGCTTGTGGAGCGCTATTACAAA AAGGAGAAACGTGTTAACATTCGAATCAAAACTTTAGATGTGATTTTGGA TATTTTCAAGTCTAATCATTATTCATTTGAAGAGGAACTAATAGATAGAA TAATTATTCCCTTCTTGGGTAGTGTTCATATGGAACCTGAATCTCGAATA AGAAGAAGGGCTATTGAAGTTTTAACTACTTTATGTTCCCTTTGTCAAAC CAACAAGAATTTAGATCTTTTGAATATCCTGGGTTGTATAATGAAAAGAT CCTTAGAACAAGAAGCTGAAAGAGATTCGCTATTGAAAGATTTACTCATC GTTGTGTATGGTTTAATTAATGTATTTAAGGACAAATTGCGCCAGCTACC AAGTACTTTTATACTGAGAGTTTTAAGTTTATTAACAACGCATTTGGAGT ATCACTATGATCACCCTAACATATTTGGTGGAAAGTCGGATATACGATAT GTAATATTTCGCTTATTTTTNNNNCTTAAGAGCTGAAGATGAGAGTTATC ATTTGGGATTGGAAGAAAGTGATGGCTCGGKGKATTTTAGTCCTTATATT ATATGCCGTAGTCCTGAAGAAGAGGAGGAAGCGGAGTTGTTGTGGAATAG ACAACAGGAAAGTGCTAGTCGTCCATCCTCTCCTTCTTGTTTATCTGGAA CAAGGCAAAAGAGCGTTTTATGTGTATCTTTGAATAAGGCCTGTTTGTTA GTGATAGAGTGTTTGGAAAGGGAAACTGATTGGAACGTTCTATGTCTCGT TCTTACCCGAGTTCCTACAGTGTTGCAAAATAGACCTCTGATATGTCGTT ATGGCAAAAGTATCAATAAATTTATCACACCACTCGTCTCTTTCACGGAT AAAAACTCAAACTTTCCCCTCCATCTGACTAATACTCCTAGTAAGTTCAG CAAATCCGATTTTCATAACCATATATTCCCTGTGCTGGCAGCTTTAGCCT CTTATAATGAATTCCTTGTCCCTGCATCACAAAGCTCTCTTATAAGAGCA TTGGAGTTAGGCTTATTATCCAAAGAATGCAATCGAATGTGTATTGTTGC CCTCACTACATCAGCCCTCGAAATGAAACGCTCAACGCGCAACCTTATAC CAGAAGTTTTATTAAACTTTTCCAAGATATCTGCAACAAAGTTTATTTCA ACACCCATTCTAGAGTTTTTATCAACAATGGTTCGATTACCCGAAGTCTT CAGTTGTTTTACTGATACAAACTATATAACTGTCTTTGCTATTGCTCTTC CTTTCACGAATCCCTTTAAGTTTGATCCCTATACAGTTTCACTGGCTCAC CATGTTATAGTGATGTGGTTTTTGAAGTGTAGACTCTCATATAGACAGAA CTTTGTTCAATTTATTATCACAAGTCTTAATTCAAACATATTCGTACCTT TTGAAGAAAATGATAAAAATAAGGATTCCATGGGACGGATGCGGAGTACG AGCTTATCAGCTGCTCGGAGGCCAAATGTGGCTCTTGCAGCTAAAAAGGA TGTTGCAGCTGATGTTCCAAAAYCCACTCAAATCCATCCATTAGCTTTTC ATCAGGAACTGACAGAAACGTGTCTCGATATTCTGGCTCGATACATGTAC GGCAATGTCTCAGTAAAGACCCGTCGCCTAAAAACATCGGATTTTATTCT TAAAAATGGGCATTCAGCGACTTGGTTGATTGGCACAATGCTTATAACTG TTTCTACGAGCTGTTGTACCCATATTGCAATGAGAAATGGTGTATGTGAT AGATGTTATTTTTATTGCATTCGAGATGGAACTTTAAGTAAGGATGATAT TACTAAAAATTCGGATAGAAGAAGACACATGTCGGATTTTTACGTAAAGC ACTTTATTTTTTATTTTTGTATTTAATAATTATTTATTTTCATTACAGTC CGGTACAAATAAGAGTCAGTTATCAAACTTAAGAAGTGATAAGGAACTTC ATAGGCACACTTCTCTGGAGAGAATGGAGTCTTCTGGAGATGATTATAGA GTAAAAAAAGTGCTACCTGAAACATGCAGTTGTTGGTGCTCTGGCTGGGC TGAAGTAATACAACCCCATGTGTACACTACCACATATTTTGCTCATGAAT TTAATTTCAGATTTACATTCGGCGTCCAAGTGGGGATGTATCCTGGATGT GTAGAGTGCAAAATACTCAGTTGCTTAGTGCGCATACACCCTCAACTAAT TTGAGAGAATTGACAGCACTCTTTGATACCTCTTTGAAAAAGGATACATT CAACTTTGATGTGTCGACTGAAAGTGATAAGATTAATGTGGATAATTTAA CGGAAGACGAATATAAGAAGTTAAGCTCGGAATTCGTACCCTCTTCAGTT TCCTCTGTATGCTTAATTCACCTATGTTTGATCTTTGTATGGAATCTTTA TTAATATATATTTGTAATTATTCTAGCCTTCTGCAGAGACGGATAAAACA GTTTCGGGGAATAGGTCGCACGATGAGGAAATGATCGTTATGTCACCTAA ATCTCCAGTTAAAAAGACAAAATCTTCACCTCCTGACACATCATGTCAAC CCCTAGAAGTCTCAGATATTAAAAGAAAGAGTTGTGATCCTATACCAGAG GTTGAAGAGGATTTACGCTCCGGAACCGATTTAAGGCGTCGACAATCTGT ACGTAGATACAACATTAATTCTTATATAAATATTAGAGTTGGATTCGAGT TAAAATTATCGAGTTCAAGATTTCGATGATTGAAGTAGAGTGAGGTTCAA GTAAATTGATGATACTCATATTTTATTTCGAGTTCACTATCAAGAAATGA AATAAATAAGATATGTTAGGTTGTTTATTAATTGGATATATACATATTTC ATATAATACCACTACTGATAATTATTGAATTCTATACAGTTAAATATTAT ATTAGGTTACCATGACGTAGTAATTAATCCTGTCTCTCTTCTTAGAAACT ATTTCCCAGTTAAATGGCTAACTTAAATAGGTTAGAAAATACGAATTTTC CTTCAGTGCTCCAATACTTAAGTGGGTCTTTATATCGATCGATACAGGGC AATTCTAAATATTGATTCTTAAATAAACATAACGTTTATAACATAAAAAT ATTATGTGGCCCATGGCCTGCGCAGTTAAAAATGTCAGTTATATACTATA AGAGTCTGGCGGCCTTAGAGCGAGTTACAATTAGTTTGAATAATTACATA AAAAATATTTGAAAATTTGTATTCCGCAGGAATAATGAAATACTCCTTGA TATTGAAAATAAATAACCGGATATTTAATTAGTGATTATTCGAGACTCAT ATAAATATTTTGAGTTTCCGTGGCTCCAAGTCAAACTATATCGAGTTTTT TCAAAAGATTTTTTGACGAGTTCGAGTAATTTACAAAAAAAAAAATAATA AAAGAAATGACGTAACTTATGTCGAGTATTTTTCGAGTTTTAGTTATAAT CGAATCCAACTCTAATAAATATATTATTCTGGCTCTTAAATTATTATTCT TCGAAATTTTTGAGGAACATGGTCCAAAAATGTTGGTTTGAATAAAAAAA TGAAGAAATATACGAAAGTTTTAATCATAATAAACCTTGTTTAGAAGATG TGCAGGCCACTTCTAAAAAAACAAAAAAAACATAGAATTCTGAACTGAAA ATAGTCCACATAAGTCGAAATAGAGCAATATATAATCAATGTGAGAAATT AAATAAGGGTACCGGTATTAATTTCAAATTTCAATGCCGAATGGATATAC AAAATTAAATGAGTTAACAAACTTTCAAAAGCTATAATGTTATATCATTA TGTCGACTATTTTCAGGTGATAATTCTATTTTTTTCTATTTAATCGTATG TTAACCGTAAAGAACTGTTGGACCATGTTCCCTGAACATTTCAAAAATAA ATAAATAATAATAATTTAAGAAACAGTCTAATATAAACATATATTTTCAT ACTAAAACTATAGGATGCATGGAGAAAATCCTTACCACCGGGTAATGAAG AAAATCAAAATAATATCCCATTGAATAGCTCTCCCAATTCTTCATTTGAA AGTAATTTAAATGAACCTGCAATGAGAACAACGCGCTCTCATACTATATC CGTATCGAGTCCGCGGAATCGGGTATTAAACAAACCTTTGCAACCTACCA CAGGAATAGTCGGTCGTCCTGGAATTGGTGGCATTGGATCTGGAGAAAAG AATGATAAAGTTTCTGGCATTTCACCGCAATTTGTATTTCTTATGCTTTA TCAATCTTCCACATTTGGAAATGCTGCTTCCCTAGAAAAACCTCTTTTAC TTCCCTCAAATAAAATTACTGAGAATAGTATTAAAAATCTTGATAGGATC CCGGCGTAAGTAAAGTTTTCTCTATTCTAGATGCATTTAAAAAGTTTAGA CGAATGTTAATTATGAATAGGAATATTTTTTTTGGGATTAAGGGGTTGAT AGTAAATTTAAAGGTTGAATAGTCATGATTGGATTACTAAACAGGATTGT CTTTTGGACACTAAGGAAAAGGACAATAAAGTTTTAGTGTATTTTTCTAT ACCAGGGGTCACGAAATGGCTCAGTGCATATTGGATTCAAAAAGAATATG CGCGCCATTTGCATATCCCAAAAAAGTACCTATAACTTGTTATGTCTTTT TGATAATGAATCGTTGATTTAGGGGTATACTTCTTATAATATTACTTAAA GATCAAACACTCAACTGCAGTATTTATTTCATCATAAGTGCTGTATCGTT ATGTATTTTGTAGATTAAAAGGAATTATGCCAACAATCCGTAAATATGGA AACTTTAGGATCGATGTGCCACCTCTAAATATTTATTGATCGAGTTCAAA TTTTGTAAAACATACATATTTTATTATCAAAAGAAACAATTTGCCGCTTT GGGAGCACTCTTTTTCCAGAAAAATTTGTTAAAAAACCCTCTAATACAAT ATTTTTTATTAATCTTCTGTTTCAATATGTAGTCTTGATCCAATGGACAA TGTCCATTAGATGGGGCCAGAGGCGCGAATATTCTTTTCAAACTAGGAGA GAACCACTACTAATATAGGGGGGGGGCTGAAGGGGTTGTAGCGCCTCCAA ATCGAGGAATTTAAAAAAAATTATAAATATAGAAGAAAAATTAGCTCCCA AAAAAATCAATTTAAGAATTCATTTTCTAAAAAAAAAATACGGGAGTTTT TTTTAGGATTTCTTTTATAAAAAGAAGGTTATTAGATCAAATTATGCTGT GAAATTAAGCACCCCTTAAAAAATATAATCCGGCGGACGCCCCTGACTTG TTATTCTTTCAATTATCCGGCCCATTATAGAAAAAGAATAGGTTACTCCT ATTCTAAACTATGTATTAATGTATACGAATCAACTCATTTTTGGAGCCGT GCCACTAACATGGCTGGAGCCTGTCTCTTGATACTCTACTTTTTCCGGCT TTTGTTTTTGTTGTTGACCCTAGGAAATTAAGAGAAGGATCCACCAGGGT ATCGCTCCCAAGGAGCCTATCCCAATTGTATATCTACAAAGCGACGTGAA ATAATTATTGATTTTTATTATATTAATTAGTCACGAAACTCACAAGATTG GAGTTCTATACATTGGACCTAATCAAGTCAATGACGAAGTTGGAATTTTA CGCAATATAAGCGGTTCTTCTAGATACACGGATTTTTTGGATGGTCTCGG AAATCTAATCAATATTAGAAATATTGATAAATCCACTCATTTCATTGGTG GATTGGATARCGAAGAGGGKGATGGKAACTTTGCTTATATGTGGGRAGAC GACGTCATGCAGGKCATTTTTCATGTTGCAACTTTAATGCCTAATCATGA TAACGATCCACAAGCCAATAAAAAGAAAAGGCATATTGGTAATAATTATK TAGCCATTGTGTACAATGATTCGCGAAATCATAGTGAGTCTTACAAAATG GGGACTGTGAGGGGGAAATTCATTTCTGCATGTATAGTCATCACTCCTCT AGATCAAGGCTCCAATAGGGTATGTGTGGATTGCAAACCAGAGCTAAAGG AACCTTTGGGACATGTCATGGTAAGTTAAAGTCAAATAATTAATTAGTTA CACAGGTCAAGAAAAAGTTACATTTCCAAAAATCGTTTACGGTCATAGCT GTATTAAGGGGTCAGGTAAGGGAAACTGAAAAAATGACCGCTTATAGCAG GTGACTAGTGTTGTGTCGATCCTTATTTAGGAGTGAAGACTGGGTGCAGT CCAGTTCAGTCCTAAAACTTGCAAAAGTTTGGTCTTTGATGATGTTATTC AACTTTTTTTGAAATCAGTTCGTGTACTGACAGTCCGAAGGACTAATAGG AATCGTCCTAAAGACTGGACTGACAACACTACAAGGGGCTTATTTTGTAA CAATTCTAAATTTGATGACTGTGTATGGCCGCTTAGTGCGGTGACACGCT TAGAACAGACCAGAATTCTACCTATTAAAATCAAATCCTCTTTTATCAAT GAAACCCTAAATATTTACACTTTAAATAAGTTCTTTTGGCATAAAACCAA CTGAAAAACCCAGATGTTATTCCTTTTACAAACAAAATTNNGTACAATTT GCGTATTCTAGCTGCAACTTGTACACAAAACAACTATTCAAAATGACACA TTTAATCTTTAGGATCCTAAAATAGTATCTGACAAGAGTTTGTCAATATT AGTTCGCCAATGGGCTCTTCATTGTAATTTGGCGGCGCAGATTCAAAAAT CTTTGAGCAATAAAATGCCTTACTCTTCAAACTGGCTCSAAAYACTTCGA GCTATTAAGCGTTTGAAAGAGAAGCTNCAAAAGGAAAATGAAATGAAGGA AGAAGAGGATCGTGAACAAGGGTCTGGGCGTCCACAACTGAATGATTTCA CAGAATTTGTCATTATGCGAAAAAAGAAAGGGAATGGCGGAAGTGGAAAT AGTGTTGGTGACTAA
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