low quality protein: twitchin-like, maker-scaffold175_size286436-snap-gene-1.53 (gene) Tigriopus kingsejongensis

Overview
Namelow quality protein: twitchin-like
Unique Namemaker-scaffold175_size286436-snap-gene-1.53
Typegene
OrganismTigriopus kingsejongensis (Tigriopus kingsejongensis)
Associated RNAi Experiments

Nothing found

Homology
[Close]
BLAST of low quality protein: twitchin-like vs. L. salmonis genes
Match: EMLSAG00000000141 (supercontig:LSalAtl2s:LSalAtl2s1021:93859:109614:1 gene:EMLSAG00000000141 transcript:EMLSAT00000000141 description:"maker-LSalAtl2s1021-augustus-gene-0.30")

HSP 1 Score: 345.125 bits (884), Expect = 2.256e-107
Identity = 153/225 (68.00%), Postives = 187/225 (83.11%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMDDGDCNMFKHGMRGCTCSAGFRHNELSAQQILTVDFGD 469
            RW+KP DDGGRPITHYIIQKKDKFGGWFDAL TDD +C A ID+LEA++PGLSEGKWYQFR++A NKAG+S PS ETKPHLCRHKNL P IDKG++ SK+++V+R+AIW IKVKGEPPP+F+WYKNG ++ ++DE +I  +EYQGGATA L V +SQM DAGTY+LVAENRNG DK DLDL+VL  M + +C+MFK G   C CS GFRH E+  + +L +DFG+
Sbjct: 1048 RWQKPSDDGGRPITHYIIQKKDKFGGWFDALKTDDASCEARIDELEARIPGLSEGKWYQFRIIAVNKAGESSPSFETKPHLCRHKNLTPSIDKGSSSSKTIKVNRTAIWQIKVKGEPPPEFTWYKNGIQIASTDEVLITREEYQGGATAKLCVSKSQMIDAGTYSLVAENRNGQDKTDLDLIVLDLMQECECDMFKTGDLECNCSNGFRHGEVGGKHLLEIDFGN 1272

HSP 2 Score: 70.4774 bits (171), Expect = 4.373e-13
Identity = 53/176 (30.11%), Postives = 81/176 (46.02%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWF---DALVTDDKACTATIDD---LEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLI-NSDEFVI----ATDEYQGGATAMLQVFRSQMHDAGTYTL 410
            W  P  DGG PITHY I+ K+K  G +   + L T D      ++    +     GL EG  Y FR+ A NK G S PS  +   L + + L+P + +    +  ++  R+  + I   GEP P  +W +NG+ L+ +SD   I        Y    T +     ++  D G YT+
Sbjct:  734 WALPESDGGAPITHYEIEYKEKSMGLWQQGEILNTSDIEIKTDVNGKPMVHGTCKGLVEGNEYVFRIKAVNKGGASLPSETSDSVLAKTRYLKPFLHQPGIYNIEIKKGRTFRYDIWFGGEPAPSVTWERNGSLLLGDSDRISIELFSKKTVYCEKNTVLTVTKANRSIDTGHYTI 909

HSP 3 Score: 57.3806 bits (137), Expect = 6.356e-9
Identity = 33/79 (41.77%), Postives = 39/79 (49.37%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKP 323
            W  P DDGG PIT Y+I+  D   G W  A+ T D   T         V GL  G  YQF V+A NK G+S P    +P
Sbjct:  950 WATPHDDGGTPITSYVIKMMDIDNGDWLXAMETKDTKAT---------VKGLKPGHIYQFEVIAVNKEGESEPCRTLEP 1019
[Close]
BLAST of low quality protein: twitchin-like vs. L. salmonis genes
Match: EMLSAG00000002571 (supercontig:LSalAtl2s:LSalAtl2s1518:43667:45350:1 gene:EMLSAG00000002571 transcript:EMLSAT00000002571 description:"maker-LSalAtl2s1518-augustus-gene-0.8")

HSP 1 Score: 139.813 bits (351), Expect = 4.157e-38
Identity = 76/147 (51.70%), Postives = 90/147 (61.22%), Query Frame = 0 
Query:  188 DDELDVGEEAGRDLMAHHHVRNGRLELNGLLG----QLGEELLAEEHVLSRLQLLLPMHLGRWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKN 330
            D  L  G     DL      R    + + L+     QLGE ++A+        + L     +W KP +DGGRPI+ YIIQKKDK G  FDALVTDD  C  TIDDLEA V GLSEGKWYQFRV+A NK+G+S PS ET+PHLC HKN
Sbjct:  157 DITLKKGRPIRYDLCKDCIKRKSDFKCDSLISEPPSQLGEPIIAD---CDNKYVTL-----KWAKPKNDGGRPISDYIIQKKDKLGDCFDALVTDDHNCVITIDDLEAXVAGLSEGKWYQFRVIAVNKSGESDPSPETRPHLCCHKN 295
[Close]
BLAST of low quality protein: twitchin-like vs. L. salmonis genes
Match: EMLSAG00000001065 (supercontig:LSalAtl2s:LSalAtl2s1177:93008:93841:-1 gene:EMLSAG00000001065 transcript:EMLSAT00000001065 description:"maker-LSalAtl2s1177-snap-gene-0.8")

HSP 1 Score: 126.716 bits (317), Expect = 2.673e-34
Identity = 64/107 (59.81%), Postives = 71/107 (66.36%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSK-SVRVHRS 350
            +W KP +D             DK GGWFDALVTDD  C  TIDDLEA VPGLSEGK YQFR +A NKAG+  PS ET+PHLCRHKNL P IDKG    K S R+ +S
Sbjct:   84 KWAKPKND-------------DKLGGWFDALVTDDHNCVVTIDDLEAXVPGLSEGKLYQFRFIAVNKAGEPDPSPETRPHLCRHKNLSPSIDKGKLAQKQSERIEQS 177
[Close]
BLAST of low quality protein: twitchin-like vs. L. salmonis genes
Match: EMLSAG00000008721 (supercontig:LSalAtl2s:LSalAtl2s541:438808:485939:-1 gene:EMLSAG00000008721 transcript:EMLSAT00000008721 description:"maker-LSalAtl2s541-augustus-gene-3.15")

HSP 1 Score: 117.857 bits (294), Expect = 3.758e-28
Identity = 65/185 (35.14%), Postives = 98/185 (52.97%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            W  PL DGG  ITH+II+KK++F   +F  + T+   C       + KV  L+E   Y+FRV A NK G  PPS  ++   C+ +N  P ID+    +  VR+      +IKV GEPP +  W+ N   + NS    I  ++Y         +  ++  D+GTY L A N+NG D+ DL+++V+ 
Sbjct: 4637 WSPPLSDGGSRITHFIIEKKEEFSSKYFKHMETETDEC-------KIKVTDLTENSKYKFRVKAVNKVGAGPPSEPSEEVTCKIRNAPPVIDRTNMDNIRVRIGEPIKLNIKVTGEPPAEKVWFLNNTIVKNSPILSITNEDYM----TRFYITNAKRGDSGTYMLTATNKNGEDEADLNILVVG 4810

HSP 2 Score: 115.546 bits (288), Expect = 1.952e-27
Identity = 68/188 (36.17%), Postives = 104/188 (55.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            +W  P+ DGGR ITHY+I++K K+   + + + T+D +       LEA V GL E + Y+FRV A NKAG S PS  T  H+C++KN +P I +      +++V +S IW + V GEP    +W ++    +I   E +  T+E      ++    R    D G Y + AEN NG D+  ++LVVL +
Sbjct: 3136 KWLAPISDGGRTITHYVIEQKGKYDLDFVEVMKTEDPS------KLEATVMGLKEKQTYEFRVKAVNKAGPSLPSEPTPKHICKYKNRKPSIIRDNLRIATIKVGKSHIWSVDVIGEPITDKTWRHEKETDVIKDHERIAITNEDYNTTFSIKNALRK---DTGKYLVKAENVNGFDEEWVELVVLGR 3314

HSP 3 Score: 112.464 bits (280), Expect = 2.308e-26
Identity = 68/185 (36.76%), Postives = 94/185 (50.81%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            W+ P  DGG PITHYIIQKK K  G  D     +       + L  KV  L E    QFRV+A NK G+SPPS  T  H  +H+ L+P ID+       V+V +  I  + + GEP P+  W Y +    I+ D   I  +EY         V +S   ++  Y + A N++GSD+  ++LVVL 
Sbjct: 3439 WKAPTSDGGSPITHYIIQKKLK--GQNDWENCGEHNTPTGQEPLTYKVESLKEKTQLQFRVIAVNKGGESPPSDPTGMHKVKHRKLKPRIDRTNLVRTVVKVGKDIILDVDIIGEPAPEIKWVYYDSKIAIDDDNIEIVNEEYN----TKFVVKKSVRKNSKRYKVTATNKHGSDEETVELVVLG 3617

HSP 4 Score: 105.916 bits (263), Expect = 2.782e-24
Identity = 62/183 (33.88%), Postives = 97/183 (53.01%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            +W KPL + G P+T+Y ++ +  FG   D  V   +       D E  + GL  GK Y+FRV A NKAG   PS  T PH  + +   P ID+    +K+V+V++  +  + V GEP P  +W+ NG ++ N+D    A   Y      ML   + +M   G YT+ A+N +G DK D+++++
Sbjct: 4040 KWAKPLRENGAPVTNYTMEYR-IFGT--DEWVKGPRVKAKKFPDGE--ITGLEPGKKYEFRVKAENKAGLGDPSEHTNPHFMKARFAPPKIDRTNLDTKTVKVNQQVVIEVDVTGEPAPDTAWFFNGEEIKNTDTKKCAHSSYH--TKLMLVPAKREM--IGKYTITAKNNSGEDKADVEIII 4213

HSP 5 Score: 103.99 bits (258), Expect = 1.185e-23
Identity = 63/183 (34.43%), Postives = 95/183 (51.91%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W+KP  DGG  IT YII+K+DK    ++   + +   T        +   + +G  Y+FRV A NKAG+S PS+ + PH  R KN  P ID+ A    ++        ++ V GEPPP+  W K+ + L +     I  +EY+      +++  S+  D G Y LVA N NG+DK   ++ VL
Sbjct: 5774 WDKPEFDGGNKITGYIIEKRDKNNPRWEVCGSTEGPGTI------GRAKKIIDGVVYEFRVKAVNKAGESDPSNPSYPHRARAKNSLPKIDRMAMMDITILAGTPLTINVPVDGEPPPKKKWTKDCSSLEDGIRVTIVNEEYK----TSIRISESKRSDKGIYELVATNINGTDKAICNVTVL 5946

HSP 6 Score: 103.99 bits (258), Expect = 1.320e-23
Identity = 62/187 (33.16%), Postives = 97/187 (51.87%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            +W+ P DDGG PIT Y+I+ +DK    W + L T+    T  I+      P + E   Y+FRV+A NKAG S  S  +     R + L+P ID+     K +RV +         GEP P  +W + NG+K+   D  ++ TD+Y       L +   +  DAG Y ++A+N  G+D  D+++ V++
Sbjct: 4336 KWKSPKDDGGSPITGYVIEMRDKDKRAWKEVLKTNGTTLTGRIES-----PAIQEDHEYEFRVIAENKAGLSEASDPSFTIKARVRFLKPRIDRSTLIKKVLRVGQLMRIDADYIGEPDPTITWFFPNGDKIKGDDRNILETDDYH----TSLVIKHVEKKDAGIYKIIAKNDQGTDTADVEVAVVS 4513

HSP 7 Score: 92.4337 bits (228), Expect = 6.329e-20
Identity = 55/173 (31.79%), Postives = 82/173 (47.40%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGS 418
            W+ P  DGG PI  +II+KK +FG W       DKAC     + + +V GL+EG  Y+FR++A N+AG   P   + P     + + P ID  A     V   +   + + + G P P+ +W  N + +  S+     T   Q      L +  S+  DAG YTL   N  G+
Sbjct: 6069 WQTPKYDGGSPIKKWIIEKKTRFGIW-------DKACEIHGSEPKGRVTGLTEGTEYEFRIIAVNEAGPGEPGEPSDPITAEARFVHPSIDLTAMQDMVVCAGQCINYTVPILGSPKPEVTWKINDSIVKQSERMDTQTSRRQ----TYLNIPFSKRTDAGYYTLEVRNEIGT 6230

HSP 8 Score: 90.5077 bits (223), Expect = 2.689e-19
Identity = 59/184 (32.07%), Postives = 85/184 (46.20%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P+D+GG  IT Y+I+KKDK+G W       ++A        +  VP L EG+ Y+FRV A N  G    S+   P  C+ +NL P ID+            S  + + V GEP P+  W  N NK I S E +            +    R +    GT  + AEN NG D  ++ + V+
Sbjct: 5476 KWIPPIDNGGSAITGYLIEKKDKYGKW-------ERAHEVPGSQNKCTVPNLVEGETYEFRVRAINAGGIGDSSNVVGPVTCKPRNLPPRIDRSTLLEVHCHAGDSFNFDVNVSGEPAPETQWLMN-NKEIESSESIKLVHHPNNTKIFVRSATRKE---NGTLLITAENVNGFDSAEILITVI 5648

HSP 9 Score: 89.7373 bits (221), Expect = 4.868e-19
Identity = 56/186 (30.11%), Postives = 89/186 (47.85%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDE--FVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W++P+DDGG PIT YII+K+ +    W      D   C  T + L        EG+ YQFR++A NKAG S P   ++    R + + P I++      +V       +   + GEPP   +W K G ++ +  +   VI    Y       L +   +  D G+Y ++A+N  G D + ++L VL
Sbjct: 5180 WKEPIDDGGAPITGYIIEKRSRNDPEWVHVQQIDGNRCKGTANHL-------IEGEEYQFRIIALNKAGPSEPGQPSRYKEARARFMPPKIERKHLHDITVSAGEMLKFEANIIGEPPADVTWKKEGKEIKSKSDKSLVITNVPYN----TKLIIRSCKKSDEGSYNVIAKNSVGKDSVTVNLKVL 5354

HSP 10 Score: 76.2554 bits (186), Expect = 8.348e-15
Identity = 55/195 (28.21%), Postives = 87/195 (44.62%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKD--KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIW----------HIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            +W+ PL DG   I HY+I+ ++    G W D   +D      +         GL +GK YQFRV + NKAG S PS  T     + + L P I++        +V R  IW           I ++GEP P+ +W  NG      +   I   EY+      + +  ++    G Y + A N +G D+ +++ +V
Sbjct: 3740 QWDFPLSDGRAAIQHYLIEMREGKAEGAWTDVGKSDGPKRFFSKG-------GLVKGKKYQFRVRSVNKAGPSEPSEPTPLVAAKPRKLAPIINRD-------QVFRDKIWCRKGQALKLEDIDIEGEPAPKVTWTFNGLDQSKLNNVTITNPEYK----TSIVIKNAERKMTGMYKITAVNEHGKDECEVEFIV 3916

HSP 11 Score: 74.3294 bits (181), Expect = 4.116e-14
Identity = 53/182 (29.12%), Postives = 76/182 (41.76%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDK-FGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSS-----ETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDK 420
            RW +P+ DGG PI  YII+K+ K    W     TD K     I+  +     L E ++YQFR++A N AG S P       + +P     K L P  D       +  +    I      G P P  +WY  G  L+  DE +  +            +   +  D+G  T+   N +GSDK
Sbjct: 6362 RWSRPISDGGSPIEGYIIEKRPKGTNSW-----TDCKNINGDIN--KGTASNLYEDEFYQFRIIAFNDAGQSEPGEPSGYIQARPRYLAPKILTPLKDINVKAGNNFTIDVEYI------GSPDPSVNWYIEGCPLVTDDERITIS---AIAPVTTFHIVNCKRTDSGETTIKLVNESGSDK 6527

HSP 12 Score: 73.1738 bits (178), Expect = 9.577e-14
Identity = 56/179 (31.28%), Postives = 81/179 (45.25%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCI--DKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFV-IATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDK 420
            +W +P +DGG  IT YI++K+   G W        KAC  T+ D+  KV GL E   Y+F V A N AG  P    ++P  C      P I  D        +  H  +I  +     P P+  W  NG ++  SDE V I  ++++    A     R++  D G Y +   N  GSD+
Sbjct: 7310 QWTRPRNDGGSYITGYIVEKRQVSGSW-------SKACHQTVSDITLKVIGLQENNDYEFHVAAINAAGQGPWGPVSEPIRCSPAKCAPKITSDLNLRDMTVIAGHDISI-TVPFFAIPQPKAKWSINGYEV--SDERVRIEINDHE----AHFYNKRAKRSDTGNYNIQLINSEGSDQ 7474

HSP 13 Score: 70.0922 bits (170), Expect = 7.126e-13
Identity = 42/129 (32.56%), Postives = 62/129 (48.06%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDD--KACTATIDDLE-AKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKN 370
            +WE+P  DGG  IT YII+K          L  DD    C      LE     G+  G+ Y FR+ A N AG   P  E+    CR+  L+  I++      +V+V  +  + + ++GEPPP  +W K+
Sbjct: 4931 KWEEPRYDGGSNITSYIIEK---------MLSNDDLWTKCGEIKPKLEFGAAKGVDLGETYNFRIRAVNAAGPGNPGPESDNLTCRYXKLKXXINRKCLIELTVKVGETIQFDVDIQGEPPPDVTWCKD 5050

HSP 14 Score: 68.9366 bits (167), Expect = 1.949e-12
Identity = 49/187 (26.20%), Postives = 77/187 (41.18%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            +WE P  DGG P+  Y+I+ +++F   W    +T +     +I +++    GL E K Y+FR+ A NKAG+  PS  TK                                      P P+  W+ N N ++  DE +      +     + +  R    D GTY LV  N +G  + + D VVL +
Sbjct: 2869 QWEPPEWDGGSPVLQYLIECREQFSTQWVKCHLTKNNGTKTSITEVQI-TEGLEENKSYEFRIKAINKAGEGVPSEPTK-------------------------------------LPDPEAQWFLNDNPIVPDDEKITIAQYTKNIVITIRKCLRK---DQGTYKLVLTNSSGKCEEEADGVVLGK 3014

HSP 15 Score: 62.3882 bits (150), Expect = 2.166e-10
Identity = 53/184 (28.80%), Postives = 76/184 (41.30%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVI-ATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P DDGG  I  YI+Q+K K    F  + T         ++L   V  LSE + Y FRV+A N+ G S PS  T       +  +P ID G      V+       ++   G P P    +     L      ++  T+E+       + V +S   DAG Y L   N +G D    ++ VL
Sbjct: 7017 WRPPRDDGGCKIKGYIVQQKHKDQSDFIDINTYPH------NELSYTVSRLSERQEYSFRVIAVNEMGQSEPSKATPNLKLGEQPNQPKIDLGYTKDIRVKAGEDFSLNLNFTGYPKPTAQLWNEDTDLKGDSRVIMQVTEEF-----VSIIVKKSTTSDAGHYRLKLANDSGYDTATFNVSVL 7189

HSP 16 Score: 60.8474 bits (146), Expect = 5.837e-10
Identity = 52/189 (27.51%), Postives = 80/189 (42.33%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKN-------LRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            WE+P  DGG  I  Y I+KK+     +  +     + T      +  +  L E + Y+FRV A N+AG S PSS ++  + +          L P  +      K+ R      +  K+ G P P+  WYK   +L +S +  I +     G T  L +      D  TY++ A N  GS     DL +
Sbjct: 8098 WERPEFDGGSRIKGYWIEKKEIGSDLWQKVNQFINSST------QINICNLIENRRYEFRVFAENEAGLSKPSSNSQSVIVKDPEDPEAPEILTPLKNTSCVEEKNAR------FTCKITGVPKPKVLWYKGARELFDSGKHEITSS----GNTYELTIKGVFGEDEDTYSVRASNSGGSSTSKADLKI 8270

HSP 17 Score: 59.6918 bits (143), Expect = 1.346e-9
Identity = 54/180 (30.00%), Postives = 81/180 (45.00%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSA-------IWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNG 417
            +W KP  DGG  +T YI+++K+ +G        D +     I  LE  V GL+E   Y+FRV+A N AG S PS+ T      +  +   +D G+A   +  +H +        I     +G+P P++ W +NG  +      +I T E  G  T  L +      D G Y   A N  G
Sbjct: 7706 KWSKPSYDGGAKVTGYIVEQKE-YGSSIWTRCNDYE-----IPTLEYGVFGLNENMEYEFRVIAVNSAGKSDPSTSTG-----YVRIGEVLD-GSAPDFTKYLHNAGAGLGQKIILACVAEGKPMPKYKWMRNGRDVSEQPGRIIMT-ESNGKFT--LTITELWEIDEGEYVCQAYNSFG 7870

HSP 18 Score: 56.6102 bits (135), Expect = 1.432e-8
Identity = 32/74 (43.24%), Postives = 40/74 (54.05%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKF--GGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPP 317
            W+ P D+GG PI +YI++K D+   G W  A  TD   C   ID       GL+E   Y+FRV A NK G S P
Sbjct: 5672 WKPPKDNGGMPIQYYIVEKCDESMGGRWTHAGQTDGPLCDFDID-------GLTENHRYKFRVRAVNKQGKSEP 5738

HSP 19 Score: 55.8398 bits (133), Expect = 1.968e-8
Identity = 34/89 (38.20%), Postives = 45/89 (50.56%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W + LDDGG PITHY+I+K D   G W +  +T          DL+  V GL   K Y  RV A N  G+S P    K  + R+++  P
Sbjct: 6264 WNESLDDGGSPITHYLIEKMDLSRGSWVEVEIT---------TDLKTIVTGLVFKKEYLMRVKAVNAIGESDPLPLDKSFIARNESDVP 6343

HSP 20 Score: 55.0694 bits (131), Expect = 4.049e-8
Identity = 33/74 (44.59%), Postives = 39/74 (52.70%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTAT-IDDLEAKVPGLSEGKWYQFRVVAANKAGDSPP 317
            W  P DDGG PITH+ I+K D   G W          C  T  DD   +V GL+  K Y+FRV A NK G+S P
Sbjct: 3036 WNYPKDDGGTPITHFSIEKMDIDTGRWV--------PCGETGPDDDTFRVDGLTRNKKYKFRVRAVNKEGESDP 3101

HSP 21 Score: 53.9138 bits (128), Expect = 8.114e-8
Identity = 32/74 (43.24%), Postives = 38/74 (51.35%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDK-FGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPP 317
            +W    DDGG+PIT YI++K DK  G W     TD        D  + KV GL EG  Y FRV A    G+S P
Sbjct: 3639 KWRPSEDDGGKPITGYIVEKIDKATGKWTPVSRTD-------ADVTDCKVKGLQEGHEYLFRVKAITDEGESEP 3705

HSP 22 Score: 53.5286 bits (127), Expect = 1.068e-7
Identity = 28/79 (35.44%), Postives = 42/79 (53.16%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKP 323
            W  P  DGG  ITHY+++++D +F  W    +     C +T       V GL+EG+ Y FR++A+N  G+ PP     P
Sbjct: 7998 WHLPEYDGGSKITHYVVERQDIRFKEW----IIIASFCKST----HFAVQGLTEGQEYNFRILASNANGNGPPLDGINP 8068

HSP 23 Score: 53.1434 bits (126), Expect = 1.518e-7
Identity = 27/88 (30.68%), Postives = 42/88 (47.73%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W  P + GGR IT Y ++K++    W        +        L   VPGL +G  Y+FR++A N+AG   PS  ++P     +  +P
Sbjct: 6917 WTPPTETGGREITGYFVEKRELGSSWV-------RVNQYPSGKLNYIVPGLIDGTRYEFRIIACNEAGPGLPSKSSEPITASVQKFKP 6997

HSP 24 Score: 52.7582 bits (125), Expect = 1.897e-7
Identity = 32/68 (47.06%), Postives = 37/68 (54.41%), Query Frame = 0 
Query:  251 DDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPP 317
            DDGG PI +Y I+K D   G W  A          T    EA+V GL EGK Y+FRV A NK G+S P
Sbjct: 4243 DDGGSPIEYYEIEKLDPNSGMWVPA---------GTSPTCEAEVKGLGEGKEYKFRVRAVNKDGESAP 4301

HSP 25 Score: 52.7582 bits (125), Expect = 2.084e-7
Identity = 35/84 (41.67%), Postives = 44/84 (52.38%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKK--DKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCR 327
            W+KP D+GG  IT Y+I+KK  D  GGW  A V   +  T T      +VP L EG   +FRV A N  G S P +  +P   R
Sbjct: 6559 WKKPKDNGGSDITSYVIEKKDLDHAGGWVPA-VNFVEPYTFT-----HRVPRLLEGTSXEFRVFAVNAQGRSIPLASDEPVTAR 6636

HSP 26 Score: 50.8322 bits (120), Expect = 6.920e-7
Identity = 32/78 (41.03%), Postives = 39/78 (50.00%), Query Frame = 0 
Query:  242 HLGRWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDL-EAKVPGLSEGKWYQFRVVAANKAGDSPP 317
            HL  W+ P DDGG PI +Y ++K D   G W          C  T+    E  V GL+EG  Y FRV A N  G+S P
Sbjct: 4533 HL-EWKPPKDDGGDPIKYYTVEKMDVDKGIWL--------PCGETLGKTPEFDVNGLTEGSLYMFRVRAVNNEGESXP 4601
[Close]
BLAST of low quality protein: twitchin-like vs. L. salmonis genes
Match: EMLSAG00000006690 (supercontig:LSalAtl2s:LSalAtl2s371:395392:401754:-1 gene:EMLSAG00000006690 transcript:EMLSAT00000006690 description:"maker-LSalAtl2s371-snap-gene-4.25")

HSP 1 Score: 87.4261 bits (215), Expect = 2.464e-18
Identity = 55/154 (35.71%), Postives = 79/154 (51.30%), Query Frame = 0 
Query:  271 WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKS-VRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDL 423
            W   LVT+D +C       EAK+  LSEG  Y+FRV A N AG S PS ET   +CR K  RP ID  +  S+  V   ++ I  +KV+G+P P+  W   G   I      ++ D      ++ L ++  +  D+G YT  A N +G D +D+
Sbjct:  737 WTKVLVTEDDSC-------EAKISDLSEGTKYRFRVRAVNSAGISMPSEETDEIICRIKKQRPTIDYSSLPSEVYVPCGQNVIIPVKVEGQPIPKKVWTYGGKIEIKQS---MSIDITNKDTSSKLVLYGGRKDDSGIYTFKASNIHGEDSVDI 880

HSP 2 Score: 80.4925 bits (197), Expect = 3.858e-16
Identity = 56/184 (30.43%), Postives = 88/184 (47.83%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG---WFDA-LVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAA-GSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDL 423
            +W+ PL DGG PI  Y +Q  +  G    W     +   K    T++DL +       G  Y+FRV+A N+ G  PPS  T P L R +   P I + A   SKSV+V++  +  I V G P P  +W+    +++ S+   I+  +     TA +    +    +G YT+ A N +G D ++ 
Sbjct:   39 KWKAPLSDGGNPINGYFLQYHEIKGNDTEWTSGEKIKPTKFPNGTLNDLIS-------GTRYEFRVLAQNRVGLGPPSEVTIPLLVRAQKSPPEISRKAVPESKSVKVNQQFVIEIPVHGVPEPDITWWHKEAQVLPSNNIKISYHQ----DTAKIIFVHAVRALSGVYTIKANNIHGEDLVEF 211

HSP 3 Score: 75.8702 bits (185), Expect = 1.158e-14
Identity = 61/193 (31.61%), Postives = 89/193 (46.11%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDD--LEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINS---DEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMD 432
            W++P+DDGG  I  Y I+ K K  G       D+     TID      K+  L +G +YQFRV+A NKAG S P             + P ID  +    +V V     + + + GEP P   W +K+ + +++S      VI T +          V RS   DAGTY L  +N +G+D    ++ VL + D
Sbjct: 1268 WKEPIDDGGAYIHGYHIEAKSKDTG-------DEWQLWETIDTNTCNGKLQNLRQGVFYQFRVIAINKAGKSEP-------------ILPYIDTKSLHDVTVEVGSRVKFDLPITGEPKPSIEWLHKDTDTVLSSTTEKSLVIETTD-NSSKIIFNSVTRS---DAGTYVLNIKNSSGTDTAKFNINVLDKPD 1436

HSP 4 Score: 63.929 bits (154), Expect = 5.236e-11
Identity = 51/193 (26.42%), Postives = 88/193 (45.60%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD----SPPSSETKPHLCRHKNLRPCIDKGA-AGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDE---FVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P +DGG  I  Y ++ ++     W   +VT   +C      + +    +S G  YQFRV A NK G+    S PSS  K  +   + ++P I++      K++   ++    I+V   P  + +W Y NG+++    E        DEY     ++  + RS   D+G +  +A N  G D++ + + VL
Sbjct:  342 WIXPQEDGGTEILEYFVEVRNTCKRNWIQGVVTKSTSC-----QISSNSGLISPGNDYQFRVAAKNKGGEISEFSSPSSTIKAEV---RFIKPKINRDILIAEKNIHAGQTLRLEIEVTSAPLAKVTWYYPNGSEVCQLQEGPKINSGIDEYGLANLSIENILRS---DSGIFKCIASNSEGLDEVQVRVEVL 523
[Close]
BLAST of low quality protein: twitchin-like vs. SwissProt
Match: gi|74966877|sp|Q23551.3|UNC22_CAEEL (RecName: Full=Twitchin; AltName: Full=Uncoordinated protein 22)

HSP 1 Score: 111.309 bits (277), Expect = 1.410e-24
Identity = 65/185 (35.14%), Postives = 101/185 (54.59%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLI-NSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+ PL DGG PI  Y I+K+ K+G W  A+       TAT       VP L+  + Y+FRVVA NK G S PS  +K  + + +NL+P ID+ A  + +++  +S  + + V GEP P  +W+   N+ I N     +   EYQ    + L V + +  D+GT+T+ A N NG D+  + + V+
Sbjct: 2891 KWDPPLSDGGAPIEEYQIEKRTKYGRWEPAITVPGGQTTAT-------VPDLTPNEEYEFRVVAVNKGGPSDPSDASKAVIAKPRNLKPHIDRDALKNLTIKAGQSISFDVPVSGEPAPTVTWHWPDNREIRNGGRVKLDNPEYQ----SKLVVKQMERGDSGTFTIKAVNANGEDEATVKINVI 3064

HSP 2 Score: 110.153 bits (274), Expect = 3.584e-24
Identity = 67/186 (36.02%), Postives = 105/186 (56.45%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKAC-TATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            WE P  DGG PIT Y+I+KK K G        D + C   + D   A++ GL EG+ YQFRV A NKAG    S  ++  + + +NL+P ID+ A  + +++V     + + V+GEPPP+  W  N  K ++  +  I +++Y+   T  +    ++ H AG YTL A N +GSDK  ++++VL +
Sbjct: 2306 WEPPKSDGGAPITQYVIEKKGKHG-------RDWQECGKVSGDQTNAEILGLKEGEEYQFRVKAVNKAGPGEASDPSRKVVAKPRNLKPWIDREAMKTITIKVGNDVEFDVPVRGEPPPKKEWIFN-EKPVDDQKIRIESEDYK---TRFVLRGATRKH-AGLYTLTATNASGSDKHSVEVIVLGK 2479

HSP 3 Score: 102.064 bits (253), Expect = 1.439e-21
Identity = 57/184 (30.98%), Postives = 96/184 (52.17%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P DDGG PI  Y+++ +   G W  A+       TAT+D       GL  G+ YQFRV A NKAG+S PS  ++  + + ++L P I++    ++ V+  ++  + + V+GEP P+  W+ NG+ L +     I  +             R+   D+G Y +VA N +G D+ ++D+ +L
Sbjct: 3482 KWTPPADDGGAPIEGYLVEMRTPSGDWVPAVTVGAGELTATVD-------GLKPGQTYQFRVKALNKAGESTPSDPSRTMVAKPRHLAPKINRDMFVAQRVKAGQTLNFDVNVEGEPAPKIEWFLNGSPLSSGGNTHIDNNTDNNTKLTTKSTARA---DSGKYKIVATNESGKDEHEVDVNIL 3655

HSP 4 Score: 101.293 bits (251), Expect = 2.510e-21
Identity = 66/182 (36.26%), Postives = 95/182 (52.20%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            W  P DDGG PI  ++I+KKDK G W +ALV      TAT       VP L EG+ YQFR+ A NKAG   PS  +   + + +NL P I +      +V+V  +  + + + GEP P  +W  NG K I   +  I  + Y     A+ +  R Q   +G YT+ A N NG+D + +++ V
Sbjct: 2600 WNAP-DDGGAPIEAFVIEKKDKNGRWEEALVVPGDQKTAT-------VPNLKEGEEYQFRISARNKAGTGDPSDPSDRVVAKPRNLAPRIHREDLSDTTVKVGATLKFIVHIDGEPAPDVTWSFNG-KGIGESKAQIENEPYI-SRFALPKALRKQ---SGKYTITATNINGTDSVTINIKV 2768

HSP 5 Score: 97.4413 bits (241), Expect = 4.947e-20
Identity = 59/183 (32.24%), Postives = 87/183 (47.54%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            WE P  DGG PI  YI++ +D          T +      + D  A + GL EGK YQFRV A NKAG   PS  ++  L + K +   +      S +V+   +  W +K+ GEP P+  W+K   +L N  +  I T + +     +    RS   D G Y L  +N +G+D+   +L VL
Sbjct: 2012 WEPPHKDGGAPIEEYIVEVRDP--------DTKEWKEVKRVPDTNASISGLKEGKEYQFRVRAVNKAGPGQPSEPSEKQLAKPKFIPAWLKHDNLKSITVKAGATVRWEVKIGGEPIPEVKWFKGNQQLENGIQLTIDTRKNEHTILCIPSAMRS---DVGEYRLTVKNSHGADEEKANLTVL 2183

HSP 6 Score: 93.2041 bits (230), Expect = 1.006e-18
Identity = 55/183 (30.05%), Postives = 83/183 (45.36%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W+ P +DGG PI  YI++KKDKFG W +    D K   AT D+       L+ G+ YQFRV A NKAG   PS  T   + + + + P ++        ++        I  +GEP P   W  N   +          D      ++ + +F +   D G Y ++ EN +G D    ++ VL
Sbjct: 3777 WKPPANDGGAPIDAYIVEKKDKFGDWVECARVDGKTTKATADN-------LTPGETYQFRVKAVNKAGPGKPSDPTGNVVAKPRRMAPKLNLAGLLDLRIKAGTPIKLDIAFEGEPAPVAKWKANDATIDTGAR----ADVTNTPTSSAIHIFSAVRGDTGVYKIIVENEHGKDTAQCNVTVL 3948

HSP 7 Score: 92.0485 bits (227), Expect = 2.651e-18
Identity = 56/184 (30.43%), Postives = 90/184 (48.91%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W+ P +DGG PI  Y+++ KD+F   W D          AT+ +L+       EG  Y+FR+ A NKAG   P S++   + + +N+ P ID+ +     V+  +    +I V GEP P  +W   G  + + D   +  ++          V R+   D GTY + AEN NG+D  ++ + VL
Sbjct: 3189 WKPPANDGGAPIEEYVVEMKDEFSPFWNDVAHVPAGQTNATVGNLK-------EGSKYEFRIRAKNKAGLGDP-SDSASAVAKARNVPPVIDRNSIQEIKVKAGQDFSLNIPVSGEPTPTITWTFEGTPVESDDRMKLNNED----GKTKFHVKRALRSDTGTYIIKAENENGTDTAEVKVTVL 3360

HSP 8 Score: 71.633 bits (174), Expect = 8.428e-12
Identity = 50/174 (28.74%), Postives = 79/174 (45.40%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKF-GGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGS 418
            WE P +DGG PIT YII++++K    W     T    C  T    + KV    E K Y +RV A NKAG   P    KP   + K   P    G      ++V  +  + + + GEP P+  W  NG  L       ++++        ++++  +   D+G +T+  +N +GS
Sbjct: 1714 WEPPEEDGGAPITGYIIERREKSEKDWHQVGQTKPDCCELT----DKKV---VEDKEYLYRVKAVNKAGPGDPCDHGKPIKMKAKKASPEFTGGGIKDLRLKVGETIKYDVPISGEPLPECLWVVNGKPLKAVGRVKMSSER----GKHIMKIENAVRADSGKFTITLKNSSGS 1876

HSP 9 Score: 70.0922 bits (170), Expect = 2.583e-11
Identity = 59/183 (32.24%), Postives = 82/183 (44.81%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W +PL DGG PI  YI++KK K G   D    +DK     + D   +V  L E + Y+FRV+A N AG+  PS  +   L   +  RP  D       +VR   +    I   G  P       NGN  I  +E  +  D   G    ++    S+  DAG Y + A N+ G D   L++ VL
Sbjct: 4757 WNRPLRDGGAPIDGYIVEKK-KLGD-NDWTRCNDKP----VRDTAFEVKNLGEKEEYEFRVIAVNSAGEGEPSKPSDLVLIEEQPGRPIFDINNLKDITVRAGETIQIRIPYAGGNPKPIIDLFNGNSPIFENERTV-VDVNPG--EIVITTTGSKRSDAGPYKISATNKYGKDTCKLNVFVL 4930

HSP 10 Score: 68.5514 bits (166), Expect = 7.063e-11
Identity = 56/186 (30.11%), Postives = 86/186 (46.24%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKV--KGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W +P  DGG PI  Y+I+K++     +       KA    I D + +V GL+  K Y+FRV A N AG    S  + P    +   RP I+ G   ++ +  +      I V     P P+ ++ K  NK+  +D  V    EY     A L + +S++ D G Y +  EN  GSD   + L V+
Sbjct: 5051 QWTRPTSDGGAPIQGYVIEKREVGSTEWT------KAAFGNILDTKHRVTGLTPKKTYEFRVAAYNAAGQGEYSVNSVPITADNAPTRPKINMGML-TRDILAYAGERAKILVPFAASPAPKVTFSKGENKISPTDPRVKV--EYS-DFLATLTIEKSELTDGGLYFVELENSQGSDSASIRLKVV 5226

HSP 11 Score: 67.0106 bits (162), Expect = 2.178e-10
Identity = 50/186 (26.88%), Postives = 90/186 (48.39%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVK--GEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            +W    ++GG P+T YI++KK+K    W +A  T     T + D+L+   PG+     Y+FRV+A N AG S PS  T P + + + L+P I      S+ +++      +++V   G P P  +W    +    + E ++        +T  +    ++  D+G Y L  +N  G D+   +++V
Sbjct: 4070 KWNPTANNGGAPVTGYIVEKKEKGSAIWTEAGKT--PGTTFSADNLK---PGVE----YEFRVIAVNAAGPSDPSDPTDPQITKARYLKPKI---LTASRKIKIKAGFTHNLEVDFIGAPDPTATWTVGDSGAALAPELLVDAKS----STTSIFFPSAKRADSGNYKLKVKNELGEDEAIFEVIV 4239

HSP 12 Score: 64.6994 bits (156), Expect = 1.304e-9
Identity = 50/186 (26.88%), Postives = 80/186 (43.01%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSK-SVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+ P D+GG P+ HY I++KD K G W        K  T+ +         + +G  Y++RVVA NKAG   PS  +     +  +  P  D    G +  V+     +  I     P P  SW K G K +   E    TD     +   L +  ++  D+G   + A N  G  + ++ + V+
Sbjct: 4363 KWDPPRDNGGSPVDHYDIERKDAKTGRWI-------KVNTSPVQGTAFSDTRVQKGHTYEYRVVAVNKAGPGQPSDSSAAATAKPMHEAPKFDLDLDGKEFRVKAGEPLVITIPFTASPQPDISWTKEGGKPLAGVE---TTD-----SQTKLVIPSTRRSDSGPVKIKAVNPYGEAEANIKITVI 4533

HSP 13 Score: 62.3882 bits (150), Expect = 6.114e-9
Identity = 32/73 (43.84%), Postives = 43/73 (58.90%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPP 317
            +W++PLDDGG PIT+Y+++K++  G W          C  T  D   KV  LSEG  Y+FRV A N+ G S P
Sbjct: 3678 KWDEPLDDGGSPITNYVVEKQEDGGRWV--------PCGET-SDTSLKVNKLSEGHEYKFRVKAVNRQGTSAP 3741

HSP 14 Score: 61.6178 bits (148), Expect = 1.157e-8
Identity = 37/95 (38.95%), Postives = 49/95 (51.58%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAA 340
            W  P DDGG PI  Y++++ +K GG   A V         +   E  VPGL E + YQFRV A N AG   PS+ ++P  C     RP ++K  A
Sbjct: 4655 WNPPRDDGGSPILGYVVERFEKRGGGDWAPVK-----MPMVKGTECIVPGLHENETYQFRVRAVNAAGHGEPSNGSEPVTC-----RPYVEKPGA 4739

HSP 15 Score: 59.3066 bits (142), Expect = 4.919e-8
Identity = 57/204 (27.94%), Postives = 86/204 (42.16%), Query Frame = 0 
Query:  240 PMHLG-----------RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKP-HLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDE--YQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            P+H+G            W  PL+DGG  IT Y ++ ++ +G     + +D       + + E  V  L E   Y+FRVVA N AG   PS  + P  +      RP I      +     +R A++  +  G P P   W +NG +L  S  +     +  Y+     +  +      DAG YT+   N  GSD    +LVV A
Sbjct: 5434 PIHVGAKSIGRNHCTITWMAPLEDGGSKITGYNVEIRE-YGSTLWTVASD-----YNVREPEFTVDKLREFNDYEFRVVAINAAGKGIPSLPSGPIKIQESGGSRPQIVVKPEDTAQ-PYNRRAVFTCEAVGRPEPTARWLRNGRELPESSRYRFEASDGVYKFTIKEVWDI------DAGEYTVEVSNPYGSDTATANLVVQA 5624
[Close]
BLAST of low quality protein: twitchin-like vs. SwissProt
Match: gi|160358754|sp|A2ASS6.1|TITIN_MOUSE (RecName: Full=Titin; AltName: Full=Connectin)

HSP 1 Score: 82.0333 bits (201), Expect = 4.002e-15
Identity = 54/178 (30.34%), Postives = 83/178 (46.63%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAK---VPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDK 420
            W  P  DGG  I +Y+++ +         L+  +K    T D+L ++   V GL EG  Y++RV A N  G   PS  TKP  C+ +   P +D       +VRV  S     +  G+P P+  W+K+   ++  D   I T       T  L+  +++  D+G Y +V EN  GS K
Sbjct: 18526 WNPPKYDGGSEIINYVLESR---------LIGTEKFHKVTNDNLLSRKYTVKGLKEGDTYEYRVSAVNIVGQGKPSFCTKPITCKDELAPPTLDLDFRDKLTVRVGESFALTGRYSGKPKPKIDWFKDEADVLEDDRTHIKTTP----TTLALEKTKAKRSDSGKYCVVVENSTGSRK 18690

HSP 2 Score: 79.7221 bits (195), Expect = 2.251e-14
Identity = 56/185 (30.27%), Postives = 86/185 (46.49%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            WEKP  DGG  I  Y+++ + K G    ++V + K C A        V GLS G+ YQFRV A N+ G S P     P + +   ++P   K    + SV+        I V G P P+ SW K+G  L  +    +     +   + +L +  S   D G Y++ A N  G+   +L ++VL +
Sbjct: 26022 WEKPEHDGGSRILGYVVEMQPK-GTEKWSVVAESKVCNAV-------VSGLSSGQEYQFRVKAYNEKGKSDPRVLGIPVIAKDLTIQPSF-KLPFNTYSVQAGEDLKIEIPVIGRPRPKISWVKDGEPLKQTTRVNVE----ETATSTILHIKESSKDDFGKYSVTATNSAGTATENLSVIVLEK 26193

HSP 3 Score: 78.9518 bits (193), Expect = 4.340e-14
Identity = 58/186 (31.18%), Postives = 90/186 (48.39%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIK--VKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            +W  PL DGG  I  Y I+K+     W     TD   C  T       V GLS G  Y+FR++A N  G  SPPS  +   + R +N+ P ++ G      + +       IK  V+G P P+ +W+K+G ++       I TD    G+T++     ++ H  G YT+ A+N +GS K ++ + V
Sbjct: 29663 KWNPPLRDGGSKIVAYSIEKRQGSDRWVRCNFTDVSECQYT-------VTGLSPGDRYEFRIIARNAVGTISPPSQSSGLIMTRDENVPPTVEFGPEYFDGLVIKSGDSLRIKALVQGRPVPRVTWFKDGVEIERRMNMEI-TDVL--GSTSLFVRDATRDH-RGVYTVEAKNVSGSTKAEVTVKV 29837

HSP 4 Score: 78.5666 bits (192), Expect = 5.036e-14
Identity = 57/186 (30.65%), Postives = 82/186 (44.09%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQK-KDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRV--HRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            WEKPL DGG  +T Y+++  K     W        K  T     LE  V  L+EG+ Y FRV A N+ G S P     P   +   + P ID      K++ V   R     I + G PPP  SW+  G+KL  S+   + T          L +  + + D G YTL  +N  G+    + +++L
Sbjct: 31444 WEKPLYDGGSRLTGYVLEACKAGTERWM-------KVVTLKPTVLEHTVISLNEGEQYLFRVRAQNEKGVSEPREIVTPVTVQDLRVLPTIDLSTMPQKTIHVPAGRPIELVIPITGRPPPTASWFFAGSKLRESERVTVETHT----KVTKLTIRETTIRDTGEYTLELKNVTGTTSETIKVIIL 31618

HSP 5 Score: 77.411 bits (189), Expect = 1.116e-13
Identity = 52/185 (28.11%), Postives = 84/185 (45.41%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +WEKP  DGG  IT Y+++ + K    W         ACT  +  LE  + GL+ G+ Y FRV A N+ G S P     P + +   ++P ++     + +V+ +      I  KG P    +W K+G  L  +    +A+ +        L +  +   D GTY L   N  GS  + + ++VL
Sbjct: 27104 KWEKPESDGGSKITGYVVEMQTKGSEKW--------SACT-QVKTLETTISGLTAGEEYVFRVAAVNEKGRSDPRQLGVPVIAKDIEIKPSVEL-PFNTFNVKANDQLKIDIPFKGRPQATVAWKKDGQVLRETTRVNVASSK----TVTTLSIKEASREDVGTYELCVSNTAGSITVPITVIVL 27274

HSP 6 Score: 77.0258 bits (188), Expect = 1.667e-13
Identity = 61/198 (30.81%), Postives = 96/198 (48.48%), Query Frame = 0 
Query:  241 MHLGRWEKPLDDGGRPITHYIIQ--KKDKFGGWFDALVTDDKACTA-TIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHI--KVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGA---TAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            +HL  W+ P +DGG P+THYI++    D  G   +A     + C    +++LE  V  L EG  Y+FRV A N+AG S PS+   P + + +   P I+      + + V      +I  K+KG P P  +W+K   K  +S E V+            T  L + +S+  D G Y++ A N  G+   ++ L VL +
Sbjct: 17817 VHLS-WKPPKNDGGSPVTHYIVECLAWDPTGKKKEAW----RQCNRRDVEELEFTVEDLIEGGEYEFRVKAVNEAGVSKPSATVGPVIVKDQTCPPAIEL----KEFMEVEEGTDVNIVAKIKGVPFPTLTWFKAPPKKPDSKEPVVYDTHVNKQVVDDTCTLVIPQSRRSDTGLYSITAVNNLGTASKEMRLNVLGR 18005

HSP 7 Score: 77.0258 bits (188), Expect = 1.901e-13
Identity = 51/171 (29.82%), Postives = 78/171 (45.61%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKS--VRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAEN 414
            W KPL DGG PIT Y ++ K        +  TD K    +    E  + GL+ G  Y FRV + NK G S PS  + P + + +   P  D  +   K+  V+   S    +  +G P P  SW K    L  +  ++ +TD     +  +L +  +  +D+G YTL  +N
Sbjct: 27893 WVKPLFDGGAPITGYTVEYKK-------SEETDWKVAIQSFRGTEYTMSGLTTGDEYVFRVRSLNKMGASDPSDSSDPQVAKEREEEPVFDVDSEMRKTLIVKAGSSFTMTVPFRGRPIPNVSWSKPDTDL-RTRAYIDSTD-----SRTLLTIENANRNDSGKYTLTIQN 28050

HSP 8 Score: 75.485 bits (184), Expect = 4.919e-13
Identity = 52/187 (27.81%), Postives = 84/187 (44.92%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMD 432
            W KP  DGG  I  YI++ + K         TD  +  A +  L+A +  L++G+ Y FRV+A N+ G S P S   P + +   + P + + A  S SV+V +     + + G P P  SW K+G  L  +    + TD         L +  +   D G Y +   N  G     ++++ L + D
Sbjct: 24940 WTKPEHDGGSKIIQYIVEMQAK--------NTDKWSECARVKSLDAVITNLTQGEEYLFRVIAVNEKGRSDPRSLAVPIIAKDLVIEPDV-RPAFSSYSVQVGQDLKIEVPISGRPKPSISWTKDGMPLKQTTRINV-TDSLD---LTTLSIKETHKDDGGQYGITVSNVVGQKTAAIEIITLDKPD 25113

HSP 9 Score: 75.485 bits (184), Expect = 5.320e-13
Identity = 58/188 (30.85%), Postives = 89/188 (47.34%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRH--KNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W KP   GG  IT Y+++K+D   G W        KA  + I + E  V GL+E   Y+FRV+A N AG  SPPS  +    CR   +  R  +D     + +++   +      V G PPP   W K+G +L  + +  I   ++   +T ++    S+  D+G Y L A N  G  K   ++ VL
Sbjct: 23165 KWAKPEYTGGFKITSYVVEKRDLPNGRWL-------KANFSNILENEFTVSGLTEDAAYEFRVIAKNAAGAISPPSEPSDAITCRDDLEAPRIMVDVRFKDTITLKAGEAFKLEADVSGRPPPTMEWTKDGKELEGTGKLEIKIADF---STHLINKDSSRT-DSGAYILTATNPGGFAKHIFNVKVL 23341

HSP 10 Score: 74.7146 bits (182), Expect = 8.013e-13
Identity = 53/189 (28.04%), Postives = 85/189 (44.97%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCID------KGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG PIT Y+++ +++           D     T+ +L+  V GL +GK Y+FRV A N  G   P + T P  C+ K + P ++      +G        V   AI    ++G P P   W  +G ++   D + + TD +    +++L +      D G Y L   N  G+  + + L VL
Sbjct: 19219 WNKPDRDGGSPITGYLVEYQEEG--------DKDWIKFKTVKNLDCVVTGLQQGKTYRFRVKAENIIGLGLPDT-TIPIECQEKLVPPSVELDVKLIEGLVVKAGTTVRFPAI----IRGVPVPTAKWTTDGTEIKTDDHYTVETDSF----SSVLTIKNCLRKDTGEYQLTVSNAAGTKTVAVHLTVL 19390

HSP 11 Score: 73.1738 bits (178), Expect = 2.807e-12
Identity = 56/188 (29.79%), Postives = 89/188 (47.34%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIK--VKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W KP+ DGG  IT YI++K+D   G W  A  T+       + + +  V GL+E + Y+FRV+A N AG  S PS  T P   + +   P I        ++ V+    + ++  V G+P P   W +   ++  S    I   +++    A+L V  +   D G Y L A N  GS    +++ VL
Sbjct: 25329 QWTKPVYDGGSMITGYIVEKRDLPEGRWMKASFTN-------VIETQFTVSGLTEDQRYEFRVIAKNAAGAISKPSDSTGPITAKDEVELPRISMDPKFRDTIVVNAGETFRLEADVHGKPLPTIEWLRGDKEIEESARCEIKNTDFK----ALLIVKDAIRIDGGQYILRASNVAGSKSFPVNVKVL 25505

HSP 12 Score: 72.7886 bits (177), Expect = 3.488e-12
Identity = 58/188 (30.85%), Postives = 87/188 (46.28%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCI--DKGAAGSKSVRVHRSAIWH-IKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W +P  DGG  +T YI++K+D     W  A   +   C  T+ D       L EG  Y+FR+ A N AG  S PS  T   +C+ +   P I  D       +V+   S +   I + G+P P+ SW + G  +  SD   I +       ++ML V  +   DAG YT+ A N  G+ +  + + VL
Sbjct: 22083 WTEPKYDGGHKLTGYIVEKRDLPSKSWMKANHVNVPDCAFTVTD-------LVEGGKYEFRIRAKNTAGAISAPSESTGTIICKDEYEAPTIVLDPTIKDGLTVKAGDSIVLSAISILGKPLPKSSWSRAGKDIRPSDIAQITSTP----TSSMLTVKYATRKDAGEYTITATNPFGTKEEHVKVSVL 22259

HSP 13 Score: 72.0182 bits (175), Expect = 6.811e-12
Identity = 56/188 (29.79%), Postives = 85/188 (45.21%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVH--RSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+KP+ DGG  IT YI++KKD   G W  A  T+       + + E  V GL E + Y+FRV+A N A + S PS  +     R +   P           + VH   + +    ++G+P P   W K+GN+L  +   +      Q     +    R+   D G YTL   N  G+  I + + VL
Sbjct: 26411 KWKKPVYDGGSKITGYIVEKKDLPDGRWMKASFTN-------VVETEFTVTGLVEDQRYEFRVIARNAADNFSEPSESSGAITARDEIDAPNASLDPKYRDVIIVHAGETFVLEADIRGKPIPDIIWSKDGNELEETAARMEIKSTLQKTTLIVKDCIRT---DGGQYTLKLSNVGGTKTIPITVKVL 26588

HSP 14 Score: 71.633 bits (174), Expect = 7.175e-12
Identity = 54/188 (28.72%), Postives = 91/188 (48.40%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG--WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP---CIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W+ P  DGG  I  YI++ + K G   W  A  T + +C  T    + KV GL +G+ Y+FRV+A N+AG+S P+   +P L + + L P    +D   A  + +RV  +      +KG P P+ +W K   +     +  +          + L++  +   D G Y+L  EN  G+  + + ++VL
Sbjct: 18922 WQPPRHDGGSKILGYIVEYQ-KVGDEEWRRANHTPE-SCPET----KYKVTGLRDGQSYKFRVLAVNEAGESDPAHVPEPVLVKDR-LEPPELILDANMAREQHIRVGDTLRLSAIIKGVPFPKVTWKKEDREAPTKAQIDVTP------VGSKLEIRNAAHEDGGIYSLTVENPAGTKTVSVKVLVL 19096

HSP 15 Score: 71.633 bits (174), Expect = 8.174e-12
Identity = 52/174 (29.89%), Postives = 82/174 (47.13%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSV--RVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNG 417
            W KP  DGG  IT Y+I+ + K         +D     +T+  LE  V  L+EG+ Y F+V+A N AG S P  E++P + + + + P +D      K V  R   +    I V G P P  +W K G++++   + V   +      + +L +      D+G Y L A+N  G
Sbjct: 22774 WPKPRHDGGSKITGYVIEAQRKG--------SDQWTHISTVKGLECVVRNLTEGEEYTFQVMAVNSAGRSAP-RESRPVIVKEQTMLPELDLRGIYQKLVIARAGDNIKVEIPVLGRPKPTVTW-KKGDQILKQTQRVNVENT---ATSTILNINECVRSDSGPYPLTAKNTVG 22934

HSP 16 Score: 71.633 bits (174), Expect = 8.537e-12
Identity = 59/189 (31.22%), Postives = 82/189 (43.39%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSS-----ETKPHLCRHKNLRPCIDKGAAGSK-SVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  PL DGG  I  YII  ++      +    D     + + DL   V GL EGK Y+FRV A N  G S P       E K  L   K L P      AG K  V  H        V G+P P   W K  ++++ S    I    ++   +++L +      D+G Y+L AEN +GSD   + + V+
Sbjct: 20608 WLPPLRDGGAKIDGYIISYRE------EDQPADRWTEYSVVKDLSLIVTGLKEGKKYKFRVAARNAVGVSMPREAEGVYEAKEQLLPPKILMPEQITIKAGKKLRVEAH--------VYGKPNPICKWKKGDDEVVTSSHLAI----HKADGSSVLIIKDVTRKDSGYYSLTAENSSGSDTQKIKVTVM 20778

HSP 17 Score: 71.2478 bits (173), Expect = 9.724e-12
Identity = 52/175 (29.71%), Postives = 77/175 (44.00%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPC---IDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNG 417
            W KPL DGG PI  Y+++ +      +D +  D+      I     +VPGL EG  Y+FR+ AAN  G+  P  E    +     L P    +D       +VRV ++     +VKG P P  +W K G  L+      +  D         LQ+  +   D G Y + A+N +G
Sbjct: 19613 WTKPLSDGGSPILGYVVEYQKPGTAQWDRINKDE-----LIRQCAFRVPGLIEGNEYRFRIRAANIVGEGEP-RELAESVIAKDILHPPEVELDVTCRDVITVRVGQTIRILARVKGRPEPDITWSKEGKVLVKDKRVDLIHDL----PRVELQIKEAVRADHGKYIISAKNSSG 19777

HSP 18 Score: 71.2478 bits (173), Expect = 1.079e-11
Identity = 60/188 (31.91%), Postives = 89/188 (47.34%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHI--KVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+KP  DGG  IT YI++KK+   G W  A  T+       I D + +V GL E   Y+FRV+A N AG  S PS  T     R +   P I        +V V     + I   + G+P P   W K   +L ++    I + ++   AT+ L V  +   D+G Y L A+N  G   + +++ VL
Sbjct: 24247 QWKKPTYDGGSKITGYIVEKKELPDGRWMKASFTN-------IIDTQFEVTGLLEDHRYEFRVIARNAAGVFSEPSESTGAITARDEVEPPRISMDPKYRDTVVVQAGESFKIDADIYGKPIPTTQWVKGDQELSSTARLEIKSTDF---ATS-LSVKDAVRVDSGNYILKAKNVAGEKSVTINVKVL 24423

HSP 19 Score: 70.4774 bits (171), Expect = 1.739e-11
Identity = 53/177 (29.94%), Postives = 81/177 (45.76%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQ-KKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPP---SSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNG 417
            +WEKP  DGG+ I  Y ++ ++     W        K+    I D +  + GL E   Y+FRV A N+ G S P   +   K  L   +   P + K  A   + ++  +A    ++ G P P   WY+ G +LI S ++ +++D    G T  L V   +  D G YT VA N  G
Sbjct: 32239 QWEKPECDGGKEILGYWVEYRQSGDSAW-------KKSNKERIKDRQFTIGGLLEATEYEFRVFAENETGLSRPRRTAMSVKTKLTSGE--APGVRKEMA-DVTTKLGEAAQLSCQIVGRPLPDIKWYRFGKELIQSRKYKMSSD----GRTHTLTVMTDEQEDEGVYTCVATNEVG 32401

HSP 20 Score: 68.9366 bits (167), Expect = 5.907e-11
Identity = 38/109 (34.86%), Postives = 54/109 (49.54%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIW 353
            W++P DDGG  IT Y +++++K G  W        +A    + DL  KV GL EG  Y+FRV A N+AG  PPS  + P L +     P     A  + + +   S  W
Sbjct: 22381 WKRPTDDGGSEITGYHVERREKKGLRWV-------RATKTPVSDLRCKVTGLQEGNTYEFRVSAENRAGIGPPSDASNPVLMKDVAYPPGPPSNAHVTDTTKKSASLAW 22482

HSP 21 Score: 68.1662 bits (165), Expect = 9.429e-11
Identity = 58/179 (32.40%), Postives = 83/179 (46.37%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKAC----TATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLR-PCIDKGAAGSKSVRVHRSAIWH--IKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNG 417
            W KP+ DGG  I  Y+++ ++K           D+ C     ATI + E  VP L  G+ Y FRV A N  G S   SET   +   + L  P ++      K+V V   A     + V G P P  +W K G  L N    +  T+ Y     ++L V +   +DAG YT+ AEN++G
Sbjct: 31151 WTKPMYDGGTDIIGYVLEMQEK---------DTDQWCRVHTNATIRNNEFTVPDLKMGQKYSFRVAAVNAKGMS-DYSETTAEIEPVERLEIPDLELADDLKKTVIVRAGASLRLMVSVSGRPSPVITWSKKGIDLANR-AIIDNTESY-----SLLIVDKVNRYDAGKYTIEAENQSG 31313

HSP 22 Score: 67.0106 bits (162), Expect = 2.299e-10
Identity = 51/183 (27.87%), Postives = 80/183 (43.72%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            WEKP  DGG  I  YI++ + K         +D  A  AT+   EA + GL +G+ Y FRV A N+ G S P   + P + +   + P   K    + +V         +   G PPP  +W+K+   L  +      + E      ++L +  +   D G YT+   N  G     L+++VL
Sbjct: 23858 WEKPEHDGGSRILGYIVEMQSKG--------SDRWATCATVKVTEATITGLIQGEEYSFRVSAQNEKGISDPRQLSVPVIAKDLVIPPAF-KLLFNTFTVLAGEDLKIDVPFIGRPPPAVTWHKDDIPLKQTTRVNAESTE----NNSLLTIKEACREDVGHYTVKLTNSAGEATETLNVIVL 24027

HSP 23 Score: 66.6254 bits (161), Expect = 2.854e-10
Identity = 54/187 (28.88%), Postives = 81/187 (43.32%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDK--ACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCI--DKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W+ P +DGG  I  YI++K  +           DK  AC   + D + +V GL EGKWY +RV A N+ G S PS  T+          P I  D       +V+          V G+P P+ +W K  + L+  D+ +   +  +     +    RS   D GTY + A N  G     +++ VL
Sbjct: 15397 WDPPKNDGGSRIKGYIVEKCPR---------GSDKWVACGEPVPDTKMEVTGLEEGKWYAYRVKALNRQGASKPSKPTEEIQAVDTQEAPEIFLDVKLLAGITVKAGTKIELPATVTGKPEPKITWTK-ADTLLKPDQRITIENVPKKSTVTITDSKRS---DTGTYIIEAVNVCGRATAVVEVNVL 15570

HSP 24 Score: 65.855 bits (159), Expect = 4.753e-10
Identity = 56/179 (31.28%), Postives = 85/179 (47.49%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCID-KGAAGSKSVRVHRSAIWHI--KVKGEPPPQFSWYKNGNKLIN--SDEFVIATDEY-QGGATAMLQVFRSQMHDAGTYTLVAENRNGS 418
            W++P  DGG PI  YII+K+      F+ +  + + C  T       V  L E + Y+FRV A N  G+S PS      + +   + P I  + A    +++V      +I   V G P P+  W KN  K+I+  +D   I  +E  +  A   L + ++   D GTYT+ A NR GS
Sbjct: 17104 WKEPRSDGGSPIQGYIIEKRRHDKPDFERV--NKRLCPTT----SFLVENLDEHQMYEFRVKAVNDVGESEPSLPLNV-VIQDDEVPPTIKLRLAVRGDTIKVKAGEPVNIPADVTGLPMPKIEWSKN-EKVIDKPTDTLNITKEEVSRSEAKTELSIPKAAREDKGTYTITASNRLGS 17274

HSP 25 Score: 65.855 bits (159), Expect = 4.921e-10
Identity = 54/187 (28.88%), Postives = 79/187 (42.25%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQ-KKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAI---WHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG PI  Y+++ K+     W    + +       +     +V GL E   YQFRV A NK G S PS     H C    L P   +  A  +   + R+ +    ++ VKG PPP+ +W K    L       I + ++       L+      +DAG Y L  EN  G  +  + + VL
Sbjct: 21398 WGKPAYDGGSPIIGYLVEVKRADSDHWVRCNLPE------KLQKTRFEVTGLMENTEYQFRVYAVNKIGYSDPSDVPDKH-CPKDILIPPEGELDAELRKTLILRAGVTMRLYVPVKGRPPPKITWSKPNVNLRERIGLDIKSTDFD----TFLRCENVNKYDAGKYILTLENSCGKKEYTIVVKVL 21573

HSP 26 Score: 65.855 bits (159), Expect = 5.094e-10
Identity = 62/207 (29.95%), Postives = 86/207 (41.55%), Query Frame = 0 
Query:  225 LLAEEHVLSRLQLLLPMHLGRWEKPLDDGGRPITHYIIQKKDK-FGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGA-AGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            L A E   S +QL        WE PL DGG PI  YII+++++    W        +     + +L  KV GL +G  Y +RV A N AG S PS    P       + P +D  A      V V       +   G P P+ +W      L   D   + T      A A L +  S+  D G YTL  EN   S   ++++ V+A
Sbjct: 15087 LNASEQTQSSVQL-------TWEPPLKDGGSPILGYIIERREEGKDNWI-------RCNMKPVPELTYKVTGLQKGNKYLYRVSAENAAGVSDPSEILGPLTADDAFVEPTMDLSAFKDGLEVIVPNPIKILVPSTGYPRPKATWTFGDQVLEEGDRVKMKTIS----AYAELVISPSERTDKGIYTLTLENPVKSISGEINVNVIA 15275

HSP 27 Score: 65.0846 bits (157), Expect = 9.993e-10
Identity = 61/193 (31.61%), Postives = 90/193 (46.63%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKF----GGWFDALVTDDKACTATI-DDLEAKVPGLSEGKWYQFRVVAANKAG-DSPPSSETKPHLCRHKNLRPC--IDKGAAGS-KSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGG-ATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P+ DGG  +  YII++K       G W          C  TI  D    V  LSEG  Y+FRV+A N AG  S  S  T P  CR +   P   +D    G   ++R     +    V G+P P+  W K G+K ++  E +  + +Y G  ATA+++       D+G YTL  +N +G+  + + + VL
Sbjct: 30748 WSAPVYDGGSKVVGYIIERKPVSEVGDGRWLK--------CNYTIVSDNFFTVTALSEGDTYEFRVLAKNAAGVISKGSESTGPVTCRDEYAPPKAELDARLQGDLVTIRAGSDLVLDAAVGGKPEPKIIWTK-GDKELDLCEKI--SLQYTGKRATAVIKY--CDRSDSGKYTLTVKNASGTKSVSVMVKVL 30927

HSP 28 Score: 63.5438 bits (153), Expect = 2.673e-9
Identity = 53/177 (29.94%), Postives = 77/177 (43.50%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHI--KVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDK 420
            W  PL DGG  IT YI++ K++     +     DK    T    +  V GL EG +Y+FRV A N AG   P   T     + + + P +   A+    + VH   +  I   V G+PPP  +W  N   L    E  I T       ++ + +   Q    G Y+L+A+N  G  K
Sbjct: 18230 WSPPLKDGGSKITGYIVEYKEEGKE--EWEKGKDKEVRGT----KLVVTGLKEGAFYKFRVRAVNVAGVGEPGEVTDVIEMKDRIVSPDLQLDASVRDRIVVHAGGVIRIIAYVSGKPPPTVTWSMNERAL--PQEAAIETT----AISSSMVIKNCQRSHQGVYSLLAKNEGGERK 18394

HSP 29 Score: 63.5438 bits (153), Expect = 3.122e-9
Identity = 53/188 (28.19%), Postives = 80/188 (42.55%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKV--KGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            W KP  DGG  IT Y+++ + K    W +A  T  K  T T++        L E   Y+FRV A N AG S P       + +   + P  D     ++ +     + + I V   G P P+ +W     +L  +D   IAT + +      L V  S   D+G Y L  EN  G     + +VV+ +
Sbjct: 30355 WLKPDHDGGSRITSYLLEMRQKGSDFWVEAGHT--KQLTFTVER-------LVENTEYEFRVKAKNDAGYSEPREAFSSVIIKEPQIEPTADLTGITNQLITCKAGSTFTIDVPISGRPAPKVTWKLEEMRLKETDRMSIATTKDR----TTLTVKDSMRGDSGRYFLTLENTAGVKTFTITVVVIGR 30529

HSP 30 Score: 63.1586 bits (152), Expect = 3.374e-9
Identity = 52/189 (27.51%), Postives = 85/189 (44.97%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQK----KDKFGGWFDALVTDDKACTATIDDLEAKVP-GLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            W+ P  +GG PI  Y ++      +K   W        +  +  I DL+ KV  G+   K Y  RV A N  G S PS  ++  + +  + +P ID        +   +  I  +  +  P P  SW+K+G ++  SD   +  D      +A L+V +S   DAG YT+  EN+ GS    +++ V+ 
Sbjct: 16094 WKPPEKNGGSPIIGYHVEMCPVGTEK---WM-------RVNSRPIKDLKFKVEEGIVPDKEYVLRVRAVNAVGVSEPSEISENVVAKDPDCKPTIDLETHDIVVIEGEKLNI-PVPFRAVPVPTVSWHKDGKEVKASDRLTMKNDHI----SAHLEVPKSVHADAGVYTITLENKLGSATASINVKVIG 16267

HSP 31 Score: 62.7734 bits (151), Expect = 4.563e-9
Identity = 32/78 (41.03%), Postives = 45/78 (57.69%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKP 323
            W  PLD+GG PIT Y ++K+++ GG + + V+        +  +E  VP L EG  YQFR +A N AG  PPS  + P
Sbjct: 17408 WTPPLDNGGSPITGYWLEKREE-GGTYWSRVSRAPITKVGLKGVEFNVPRLIEGVKYQFRAMAINAAGIGPPSEPSDP 17484

HSP 32 Score: 62.7734 bits (151), Expect = 5.193e-9
Identity = 51/187 (27.27%), Postives = 82/187 (43.85%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSV--RVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W++P  DGG  IT YI++++D   G W        KA    + + +  V GL++   Y+FRV A N  G  S PS    P  C      P ID     ++ V  R   S      + G+P P   WYK+ +K + ++  V   +       A + +  +   ++G+Y L   N  GS    + + +L
Sbjct: 28577 WQQPAFDGGSKITGYIVERRDLPDGRW-------TKASFTNVIETQFTVSGLTQNSQYEFRVFARNAVGSVSNPSEVVGPITCIDSYGGPVIDLPLEYTEVVKYRAGTSVKLRAGISGKPEPTIEWYKD-DKELQTNALVCVENSTD---LASILIKDANRLNSGSYELKLRNAMGSASATIRVQIL 28752

HSP 33 Score: 62.3882 bits (150), Expect = 6.610e-9
Identity = 50/177 (28.25%), Postives = 75/177 (42.37%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSV--RVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDK 420
            W KP+ DGG  +T Y+++ K      +  + T  +  T      E  V  L  G  Y F+V A N AG   P + T+P   +     P ID   A   SV  +        I  KG PPP  +W K+   L +   + I   +    ++++L + +   +D G Y L  EN  G  K
Sbjct: 28976 WSKPVYDGGSDVTGYVVEMKQGDEEEWTIVSTRGEVRTT-----EYVVSNLKPGVNYYFQVSAVNCAGQGEPITMTEPAQAKDVLEEPEIDLDVALRTSVIAKAGEDVQLLIPFKGRPPPTVTWRKDEKNLGSDTRYSIQNTD----SSSLLVIPQVTRNDTGKYILTIENGVGQPK 29143

HSP 34 Score: 62.003 bits (149), Expect = 8.415e-9
Identity = 59/187 (31.55%), Postives = 78/187 (41.71%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQK-KDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAG--DSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYK-NGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W +P+ DGG  I  YII+  K   G W             T+ DL+A       G+ Y+FRV A N AG  DS   + T   + R       ID     +  VR   S    I  +G P P   W K + N  I +D  +  TD +     + L V     +DAG YTL  EN +G   I   + VL
Sbjct: 30063 WARPVFDGGMEIIGYIIEMCKADLGDWHKVNTEPCVKTRYTVTDLQA-------GEEYKFRVSAVNGAGKGDSCEVTGTIKAVDRLSAPELDIDANFKQTHIVRAGVSIRLFIAYQGRPTPTAVWSKPDSNLSIRAD--IHTTDSF-----STLTVENCNRNDAGKYTLTVENNSGKKSITFTVKVL 30235

HSP 35 Score: 61.6178 bits (148), Expect = 9.826e-9
Identity = 32/76 (42.11%), Postives = 43/76 (56.58%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSET 321
            W +P+DDGG  I  YI++K+DK G      +   K    T+ DL  +V GL+EG  Y+FRV A N AG   PS  +
Sbjct: 26711 WTRPVDDGGAEIEGYILEKRDKEG------IRWTKCNKKTLTDLRFRVTGLTEGHSYEFRVAAENAAGVGEPSEPS 26780

HSP 36 Score: 61.6178 bits (148), Expect = 1.090e-8
Identity = 50/186 (26.88%), Postives = 74/186 (39.78%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSK--SVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W +P  DGG  I  Y ++     G  W     T  +     I   E    GL E   YQFR +A      S PS  + P     +N+ P I+         +V+   +      V G+P P  SW K+   +  ++   +A           L++F     D+G YT+ AEN +GS    + L VL
Sbjct: 20999 WARPKHDGGSRIIGYFVEACKLPGDKWVRCNTTPHQ-----IPQEEYTATGLEENAQYQFRAIAKTAVNISQPSEPSDPVTILAENVPPRIELSVEMKSLLTVKAGTNVCLDATVFGKPMPTVSWKKDTTPIKQAEGIKMAMKR----NLCTLELFSVNRKDSGDYTITAENSSGSKSATIKLKVL 21175

HSP 37 Score: 60.4622 bits (145), Expect = 2.285e-8
Identity = 51/178 (28.65%), Postives = 79/178 (44.38%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETK--PHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNG--NKLINSDEFVIATDEYQGGA--TAMLQVFRSQMHDAGTYTLVAENRNG 417
            W+ P  DGG PI  YI++ +++    +  +   DK  TA     E  VP L E + Y+FRV A N+AG+S PS  T   P     +     ID GA      +          +KG P P+ SW  +G   K +      I  D     A  ++++ +       +G Y++ A+N+ G
Sbjct: 17514 WKPPAKDGGSPIKGYIVEMQEEGTTDWKPVNEPDKLLTAC----ECVVPNLKELRKYRFRVKAVNEAGESEPSDTTGEIPATDIQEVPEVFIDIGAQDCLVCKAGSQVKIPAVIKGRPTPKSSWEFDGKAKKAMKDGVHDIPEDAQLETAENSSVIIIPECTRAHSGKYSITAKNKAG 17687

HSP 38 Score: 60.4622 bits (145), Expect = 2.345e-8
Identity = 50/181 (27.62%), Postives = 81/181 (44.75%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCID--------KGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGS 418
            W+ P  DGG  I +Y +++++K    + A+ T++      I +    V  L EG  Y+FRV   N  G+S  S  ++P       + P  D        K    + +VR   +A    KV G P P   WY+ G ++I +D       E++GG   ++ +      DA  Y + A N+ GS
Sbjct: 32634 WKPPASDGGAKIRNYYLERREKKQNKWIAVTTEE------IRETVFSVQNLIEGLEYEFRVKCENLGGESEWSEISEP-------VTPKSDVPIQAPHFKEELRNLNVRYQSNATLVCKVTGHPKPIVKWYRQGKEII-ADGLKYRIQEFKGGYHQLI-IASVTDDDATVYQVRATNQGGS 32799

HSP 39 Score: 60.4622 bits (145), Expect = 2.810e-8
Identity = 55/189 (29.10%), Postives = 81/189 (42.86%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKF-GGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWH--IKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATA---MLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG  I HY+++  +K    W            A +      V GL E   Y FRV A N+AG S P     P L + +   P ID     S +V V   +     I + G+P P+ +  ++G  L        AT  +    TA    + +  S   DAG Y + A N +G+ K  ++++VL
Sbjct: 28184 WLKPEHDGGSRIIHYVVEALEKGQKTWVKC---------AVVKTTHHVVSGLRESHEYFFRVFAENQAGLSDPRELLLPVLIKDQLEPPEIDMKNFPSHTVYVRAGSNLKVDIPISGKPLPKVTLSRDGVPL-------KATMRFNTEITAENLTINLKESVTTDAGRYEITAANSSGTTKTFINIIVL 28356

HSP 40 Score: 60.077 bits (144), Expect = 2.984e-8
Identity = 33/83 (39.76%), Postives = 43/83 (51.81%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKP-HLCR 327
            W  P  DGG PIT Y +++++  G W        +     I DL+ +V GL EG  Y+FRV A N AG S PS  + P   CR
Sbjct: 19513 WTLPKSDGGSPITGYYVERREITGKWV-------RVNKTPIADLKFRVTGLYEGNTYEFRVFAENLAGLSNPSPSSDPIKACR 19588

HSP 41 Score: 60.077 bits (144), Expect = 3.142e-8
Identity = 50/187 (26.74%), Postives = 81/187 (43.32%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKF-GGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKG--AAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            W KP  DGG  IT Y++++K K    W  A ++  K C       E ++  L E    +FRV A N+ G S P +   P   +   + P +D         SVR+  +    +  KG+P P  SW K+G  L  S+    +  E +      L +  S+    G YT++ +N    +   + ++ L 
Sbjct: 29272 WTKPDFDGGSIITDYLVERKGKGEQAWSHAGIS--KTC-------EIEIGQLKEQSVLEFRVSARNEKGQSDPVT-IGPLTVKELVITPEVDLSEIPGAQISVRIGHNVHLELPYKGKPKPSISWLKDGLPLKESEYVRFSKTENK----ITLSIKNSKKEHGGKYTVILDNAVCRNSFPITIITLG 29444

HSP 42 Score: 60.077 bits (144), Expect = 3.425e-8
Identity = 36/85 (42.35%), Postives = 48/85 (56.47%), Query Frame = 0 
Query:  243 LGRWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCR 327
            L +WE PLDDGG  I +Y ++KKDK     D +V      T+T+ + +  V  L EGK Y FRV A N+ G  PP   +KP L +
Sbjct: 16608 LCKWEPPLDDGGSEIINYTLEKKDKTKPDSDWIVI-----TSTLRNCKYSVTKLIEGKEYLFRVRAENRFGPGPPCV-SKPLLAK 16686

HSP 43 Score: 60.077 bits (144), Expect = 3.575e-8
Identity = 52/188 (27.66%), Postives = 80/188 (42.55%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIK--VKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W KP  DGG  IT YI+++++   G W     T+       + +   +V  L+E + Y+FRV A N A   S PS  T P   +     P I         + V    +  I   + G P P  SW K+G ++    +  I + +Y    T  L V      D G Y L  +N  G+  + ++  VL
Sbjct: 27493 KWSKPRYDGGAKITGYIVERRELPDGRWLKCNFTN-------VQETYFEVTELTEDQRYEFRVFARNAADSVSEPSESTGPITVKDDVEAPRIMMDVKFRDVIIVKAGEVLKINADIAGRPLPVISWAKDGVEIEERAKTEIVSTDY----TTTLTVKDCVRRDTGQYVLTLKNVAGTRTMAVNCKVL 27669

HSP 44 Score: 60.077 bits (144), Expect = 3.733e-8
Identity = 49/187 (26.20%), Postives = 78/187 (41.71%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACT--ATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWH--IKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP+ DGG P+  Y+I+ K        A   +   CT  + +   +  V  L E   Y FR+ A N  G   P++     + + +   P I+  A   K V +  SA     + +KG P P+  W K    L    +  + +      +  ML +      D+G Y L  EN +GS    +++ VL
Sbjct: 26812 WNKPIYDGGAPVRGYVIELK-------KAAADEWTTCTPPSGLQGKQFTVTKLKENTEYNFRICAFNTEGVGEPATIPGSVVAQERMEAPEIELDADLRKVVTLRASATLRLFVTIKGRPEPEVKWEKAEGILTERAQIEVTS------SYTMLVIDNVTRFDSGRYNLTLENNSGSKTAFVNVRVL 26985

HSP 45 Score: 59.3066 bits (142), Expect = 5.176e-8
Identity = 32/77 (41.56%), Postives = 45/77 (58.44%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETK 322
            W++P  DGG PIT Y+I++K++     ++L+   KA    +   E    GL EG  Y FR+ A NKAG SPPS  T+
Sbjct: 20901 WDRPDSDGGSPITGYLIERKER-----NSLLWV-KANDTIVRSTEYPCAGLVEGLEYSFRIYALNKAGSSPPSKPTE 20971

HSP 46 Score: 59.3066 bits (142), Expect = 5.839e-8
Identity = 33/85 (38.82%), Postives = 47/85 (55.29%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHK 329
            W+ P DDGG P+ +YI++K+D + G W           +A    L+ KVP L +G  Y FRV A NK G  PP  ++ P + +HK
Sbjct: 19414 WQPPKDDGGSPVINYIVEKQDTRKGTW--------GVVSAGSSKLKLKVPHLQKGCEYVFRVKAENKMGVGPP-LDSIPTVAKHK 19489

HSP 47 Score: 59.3066 bits (142), Expect = 5.991e-8
Identity = 32/81 (39.51%), Postives = 42/81 (51.85%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATID---DLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKP 323
            WE P DDGG P+T Y+++K+D             K  T  +D   DLE  VP L +GK Y F+V A NK G   P+   +P
Sbjct: 15297 WEPPDDDGGSPLTGYVVEKRD----------MSRKTWTKVMDFVTDLEFTVPDLVQGKEYLFKVCARNKCGPGEPAYTDEP 15367

HSP 48 Score: 58.9214 bits (141), Expect = 7.689e-8
Identity = 57/194 (29.38%), Postives = 83/194 (42.78%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG------GWFDALVTDDKACTAT--IDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPC-IDKGAAGSKSVRVHRSAIWHI--KVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP+ DGG PI  Y+++ + K        GW        K C A   +  +E  V  L E + Y+FRV A N+ G   P +E K  +   + L P  ID  A+  K V V       +   V+G P P+ +W K G   +     V   D       A L +  S   D+G Y+L   N  G   + +++ VL
Sbjct: 20306 WGKPVYDGGAPIIGYVVEMRPKIADASPDEGW--------KRCNAAAQLIRMEFTVTSLDENQEYEFRVCAQNQVGIGRP-AELKEAIKPKEILEPPEIDLDASMRKLVVVRAGCPIRLFAIVRGRPAPKVTWRKVGIDNVVRKGQVDLVD-----TMAFLVIPNSTRDDSGKYSLTLVNPAGEKAVFVNVKVL 20485

HSP 49 Score: 58.9214 bits (141), Expect = 8.236e-8
Identity = 33/78 (42.31%), Postives = 41/78 (52.56%), Query Frame = 0 
Query:  241 MHLGRWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPP 317
            M L  W  P DDGG  IT +II++KD K   W   + T+   C  T         GL EG+ Y+FRV+A NK G  PP
Sbjct: 17001 MCLLNWSDPADDGGSDITGFIIERKDAKMHTWRQPIETERSKCDIT---------GLIEGQEYKFRVIAKNKFGCGPP 17069

HSP 50 Score: 58.5362 bits (140), Expect = 9.370e-8
Identity = 35/79 (44.30%), Postives = 46/79 (58.23%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKP 323
            W  P  DGG  I +YI++++DK G  W      + KA T    DL  KV GL+EG  Y+FR++A N AG S PS+ T P
Sbjct: 23464 WGHPDSDGGSEIINYIVERRDKAGQRWVKC---NKKALT----DLRFKVSGLTEGHEYEFRIMAENAAGISAPSA-TSP 23534

HSP 51 Score: 58.5362 bits (140), Expect = 1.030e-7
Identity = 33/79 (41.77%), Postives = 42/79 (53.16%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETK 322
            +WE P +DGGRPI  YII+KK+K G  W  A  T    C   + D       + EG   QF+V A N+AG   PS  T+
Sbjct: 15992 KWEPPKNDGGRPIQRYIIEKKEKLGTRWVKAGKTSGPDCNFRVTD-------VIEGTEVQFQVRAENEAGVGHPSEPTE 16063

HSP 52 Score: 58.151 bits (139), Expect = 1.244e-7
Identity = 52/178 (29.21%), Postives = 76/178 (42.70%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACT--ATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSK--SVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGS 418
            W KP+ DGG  I  YI++K D   G W          CT    I+    +V  L E   Y FR+ A NKAG    +    P +   K   P ID      K  ++R   S    + +KG P P+  W K     ++ D    A  +     T+++ +     +D+G YTL  EN +G+
Sbjct: 24647 WTKPIYDGGCEIQGYIVEKCDVSVGEW--------TMCTPPTGINKTNLEVEKLLEKHEYNFRICAINKAGVGEHADVPGPVMVEEKLEAPDIDLDLELRKVINIRAGGSLRLFVPIKGRPTPEVKWGK-----VDGDIRDAAIIDVTSSFTSLV-LDNVNRYDSGKYTLTLENSSGT 24810

HSP 53 Score: 57.7658 bits (138), Expect = 1.929e-7
Identity = 46/185 (24.86%), Postives = 73/185 (39.46%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            W+KP  DGG  IT Y++    +   W   +    K+ +     L+     L EGK Y FRV A N+ G+  PS                +          + + +    I +KG+P P  SW K  + L       + +          L V+  Q  DAG YT+  +N  G+ +  L + V+ +
Sbjct: 21693 WKKPRSDGGSRITGYVVDFLTEENKWQRVM----KSLS-----LQYSTKDLKEGKEYTFRVSAENENGEGTPSEIVVVAKDDVVAPDLDLKDLPDLCYLAKENSNFRLKIPIKGKPAPSVSWKKGEDPLATDTRVSVEST----AVNTTLVVYDCQKSDAGKYTITLKNVAGTKEGTLSIKVVGK 21864

HSP 54 Score: 56.9954 bits (136), Expect = 2.697e-7
Identity = 30/77 (38.96%), Postives = 40/77 (51.95%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETK 322
            W+ P DDGG PIT Y+++K++         V   K     + +   K  GL EG  Y+FRV A NKAG   PS  +K
Sbjct: 21297 WKAPADDGGSPITGYLVEKRET------QAVNWTKVNRKPVIERTLKATGLQEGTEYEFRVTAINKAGPGKPSDASK 21367

HSP 55 Score: 56.225 bits (134), Expect = 5.644e-7
Identity = 32/89 (35.96%), Postives = 45/89 (50.56%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            +W KP  DGG  I++Y++ K++K    +  +  D       + D   KV  L EG  YQFRV A N AG+S PS  +    CR  +  P
Sbjct: 31048 QWTKPESDGGNEISNYLVDKREKKSLRWTRVNKD-----YVVYDTRLKVTSLMEGCDYQFRVTAVNAAGNSEPSEASNFISCREPSYTP 31131

HSP 56 Score: 56.225 bits (134), Expect = 5.742e-7
Identity = 37/89 (41.57%), Postives = 45/89 (50.56%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTAT-IDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W KP  DGG  I  Y+I+   K G       TD+    A  +   E  + GL EG+ Y FRV A NKAG+S PS  + P LCR K   P
Sbjct: 15793 WTKPEHDGGAKIESYVIEML-KTG-------TDEWVRVAEGVPTTEHLLTGLMEGQEYSFRVRAVNKAGESEPSEPSDPVLCREKLYPP 15873

HSP 57 Score: 55.4546 bits (132), Expect = 8.306e-7
Identity = 30/78 (38.46%), Postives = 43/78 (55.13%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKP 323
            W+ PL DGG  IT+YI++K++     +  + T    CT        KV GL+EG  Y FRV+A N+ G   P+  T+P
Sbjct: 22677 WDLPLIDGGSRITNYIVEKREATRKSYSTVTTKCHKCT-------YKVTGLTEGCEYFFRVMAENEYGVGEPTETTEP 22747

HSP 58 Score: 55.4546 bits (132), Expect = 8.522e-7
Identity = 31/76 (40.79%), Postives = 41/76 (53.95%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSET 321
            WE P  DGG  IT+Y+I+ +DK        +  D A T   +DL A V  + EG+ Y FRV A N+ G   PS+ T
Sbjct: 15000 WEPPEYDGGAEITNYVIELRDKTS------IRWDTAMTVRAEDLSATVTDVVEGQEYSFRVRAQNRIGVGKPSAAT 15069
[Close]
BLAST of low quality protein: twitchin-like vs. SwissProt
Match: gi|384872704|sp|Q8WZ42.4|TITIN_HUMAN (RecName: Full=Titin; AltName: Full=Connectin; AltName: Full=Rhabdomyosarcoma antigen MU-RMS-40.14)

HSP 1 Score: 81.6481 bits (200), Expect = 5.735e-15
Identity = 53/178 (29.78%), Postives = 84/178 (47.19%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAK---VPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDK 420
            W  P  DGG  I +Y+++ +         L+  +K    T D+L ++   V GL EG  Y++RV A N  G   PS  TKP  C+ +   P +        ++RV  +     +  G+P P+ SW+K+   ++  D   I T      AT  L+  +++  D+G Y +V EN  GS K
Sbjct: 17664 WNPPKYDGGSEIINYVLESR---------LIGTEKFHKVTNDNLLSRKYTVKGLKEGDTYEYRVSAVNIVGQGKPSFCTKPITCKDELAPPTLHLDFRDKLTIRVGEAFALTGRYSGKPKPKVSWFKDEADVLEDDRTHIKTTP----ATLALEKIKAKRSDSGKYCVVVENSTGSRK 17828

HSP 2 Score: 78.9518 bits (193), Expect = 3.907e-14
Identity = 58/186 (31.18%), Postives = 90/186 (48.39%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIK--VKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            +W  PL DGG  I  Y I+K+     W     TD   C  T       V GLS G  Y+FR++A N  G  SPPS  +   + R +N+ P ++ G      + +       IK  V+G P P+ +W+K+G ++       I TD    G+T++     ++ H  G YT+ A+N +GS K ++ + V
Sbjct: 28801 KWNPPLRDGGSKIVGYSIEKRQGNERWVRCNFTDVSECQYT-------VTGLSPGDRYEFRIIARNAVGTISPPSQSSGIIMTRDENVPPIVEFGPEYFDGLIIKSGESLRIKALVQGRPVPRVTWFKDGVEIEKRMNMEI-TDVL--GSTSLFVRDATRDH-RGVYTVEAKNASGSAKAEIKVKV 28975

HSP 3 Score: 78.9518 bits (193), Expect = 4.046e-14
Identity = 61/188 (32.45%), Postives = 86/188 (45.74%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIK--VKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W KP   GG  IT YI++K+D   G W        KA  + I + E  V GL+E   Y+FRV+A N AG  SPPS  +    CR     P I        +V +     + ++  V G PPP   W K+G +L  + +  I   ++    +  L    S   D+G YTL A N  G  K   ++ VL
Sbjct: 22303 KWAKPEYTGGFKITSYIVEKRDLPNGRWL-------KANFSNILENEFTVSGLTEDAAYEFRVIAKNAAGAISPPSEPSDAITCRDDVEAPKIKVDVKFKDTVILKAGEAFRLEADVSGRPPPTMEWSKDGKELEGTAKLEIKIADF----STNLVNKDSTRRDSGAYTLTATNPGGFAKHIFNVKVL 22479

HSP 4 Score: 78.5666 bits (192), Expect = 4.778e-14
Identity = 54/185 (29.19%), Postives = 85/185 (45.95%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            WEKP  DGG  +  Y+++ + K G    ++V + K C A        V GLS G+ YQFRV A N+ G S P     P + +   ++P + K    + S++        I V G P P  SW K+G  L  +    +     +   + +L +      D G YT+ A N  G+   +L ++VL +
Sbjct: 25160 WEKPEHDGGSRVLGYVVEMQPK-GTEKWSIVAESKVCNAV-------VTGLSSGQEYQFRVKAYNEKGKSDPRVLGVPVIAKDLTIQPSL-KLPFNTYSIQAGEDLKIEIPVIGRPRPNISWVKDGEPLKQTTRVNVE----ETATSTVLHIKEGNKDDFGKYTVTATNSAGTATENLSVIVLEK 25331

HSP 5 Score: 78.5666 bits (192), Expect = 5.496e-14
Identity = 56/186 (30.11%), Postives = 82/186 (44.09%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQK-KDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRV--HRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            WEKPL DGG  +T Y+++  K     W        K  T     LE  V  L+EG+ Y FR+ A N+ G S P         +   + P ID      K++ V   R     I + G PPP  SW+  G+KL  S+   + T        A L +  + + D G YTL  +N  G+    + +++L
Sbjct: 30582 WEKPLYDGGSRLTGYVLEACKAGTERWM-------KVVTLKPTVLEHTVTSLNEGEQYLFRIRAQNEKGVSEPRETVTAVTVQDLRVLPTIDLSTMPQKTIHVPAGRPVELVIPIAGRPPPAASWFFAGSKLRESERVTVETHT----KVAKLTIRETTIRDTGEYTLELKNVTGTTSETIKVIIL 30756

HSP 6 Score: 78.5666 bits (192), Expect = 5.998e-14
Identity = 51/185 (27.57%), Postives = 84/185 (45.41%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTAT-IDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +WEKP  DGG  IT Y+++ + K           +K  T T +  LEA + GL+ G+ Y FRV A N+ G S P     P + R   ++P ++     + +V+        +  KG P    +W K+G  L  +    +++ +        L +  +   D GTY L   N  GS  + + ++VL
Sbjct: 26242 KWEKPESDGGSKITGYVVEMQTK---------GSEKWSTCTQVKTLEATISGLTAGEEYVFRVAAVNEKGRSDPRQLGVPVIARDIEIKPSVEL-PFHTFNVKAREQLKIDVPFKGRPQATVNWRKDGQTLKETTRVNVSSSK----TVTSLSIKEASKEDVGTYELCVSNSAGSITVPITIIVL 26412

HSP 7 Score: 77.7962 bits (190), Expect = 8.811e-14
Identity = 54/189 (28.57%), Postives = 87/189 (46.03%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCID------KGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG PIT Y+++ +++         T D     T+ +LE  V GL +GK Y+FRV A N  G   P + T P  C+ K + P ++      +G        V   AI    ++G P P   W  +G+++   + + + TD +    +++L +      D G Y +   N  GS  + + L VL
Sbjct: 18357 WNKPDRDGGSPITGYLVEYQEEG--------TQDWIKFKTVTNLECVVTGLQQGKTYRFRVKAENIVGLGLPDT-TIPIECQEKLVPPSVELDVKLIEGLVVKAGTTVRFPAI----IRGVPVPTAKWTTDGSEIKTDEHYTVETDNF----SSVLTIKNCLRRDTGEYQITVSNAAGSKTVAVHLTVL 18528

HSP 8 Score: 76.2554 bits (186), Expect = 2.940e-13
Identity = 55/185 (29.73%), Postives = 83/185 (44.86%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKV--KGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KPL DGG PIT Y ++ K           TD K    ++   E  + GL+ G  Y FRV + NK G S PS  + P + + +   P  D  +   K++ V   A + + V  +G P P   W K    L  +  +V  TD     +   L +  +  +D+G YTL  +N   +  + L + VL
Sbjct: 27031 WVKPLFDGGAPITGYTVEYKKSDD-------TDWKTSIQSLRGTEYTISGLTTGAEYVFRVKSVNKVGASDPSDSSDPQIAKEREEEPLFDIDSEMRKTLIVKAGASFTMTVPFRGRPVPNVLWSKPDTDL-RTRAYVDTTD-----SRTSLTIENANRNDSGKYTLTIQNVLSAASLTLVVKVL 27202

HSP 9 Score: 75.8702 bits (185), Expect = 3.819e-13
Identity = 61/197 (30.96%), Postives = 96/197 (48.73%), Query Frame = 0 
Query:  241 MHLGRWEKPLDDGGRPITHYIIQ--KKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHI--KVKGEPPPQFSWYKNGNKLINSDEFVI---ATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            +HL  W+ P +DGG P+THYI++    D  G   +A    +K     +++L+  V  L EG  Y+FRV A N AG S PS+   P   + +   P ID      + + V      +I  K+KG P P  +W+K   K  ++ E V+     ++     T  L + +S+  D G YT+ A N  G+   ++ L VL +
Sbjct: 16955 VHLS-WKPPKNDGGSPVTHYIVECLAWDPTGTKKEAWRQCNKR---DVEELQFTVEDLVEGGEYEFRVKAVNAAGVSKPSATVGPVTVKDQTCPPSIDL----KEFMEVEEGTNVNIVAKIKGVPFPTLTWFKAPPKKPDNKEPVLYDTHVNKLVVDDTCTLVIPQSRRSDTGLYTITAVNNLGTASKEMRLNVLGR 17143

HSP 10 Score: 74.7146 bits (182), Expect = 7.805e-13
Identity = 60/188 (31.91%), Postives = 89/188 (47.34%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHI--KVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+KP  DGG  IT YI++KK+   G W  A  T+       I D   +V GL E   Y+FRV+A N AG  S PS  T     R +   P I        ++ VH    + +   + G+P P   W K   +L N+    I + ++   AT+ L V  +   D+G Y L A+N  G   + +++ VL
Sbjct: 23385 QWKKPTYDGGSKITGYIVEKKELPEGRWMKASFTN-------IIDTHFEVTGLVEDHRYEFRVIARNAAGVFSEPSESTGAITARDEVDPPRISMDPKYKDTIVVHAGESFKVDADIYGKPIPTIQWIKGDQELSNTARLEIKSTDF---ATS-LSVKDAVRVDSGNYILKAKNVAGERSVTVNVKVL 23561

HSP 11 Score: 73.559 bits (179), Expect = 2.052e-12
Identity = 52/187 (27.81%), Postives = 84/187 (44.92%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMD 432
            W KP  DGG  I  YI++ + K         ++  +  A +  L+A +  L++G+ Y FRVVA N+ G S P S   P + +   + P + K A  S SV+V +     + + G P P  +W K+G  L  +    + TD         L +  +   D G Y +   N  G     +++V L + D
Sbjct: 24078 WTKPEHDGGSKIIQYIVEMQAKH--------SEKWSECARVKSLQAVITNLTQGEEYLFRVVAVNEKGRSDPRSLAVPIVAKDLVIEPDV-KPAFSSYSVQVGQDLKIEVPISGRPKPTITWTKDGLPLKQTTRINV-TDSLD---LTTLSIKETHKDDGGQYGITVANVVGQKTASIEIVTLDKPD 24251

HSP 12 Score: 73.1738 bits (178), Expect = 2.640e-12
Identity = 56/188 (29.79%), Postives = 88/188 (46.81%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCI--DKGAAGSKSVRVHRSAIWH-IKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W +P  DGG  +T YI++K+D     W  A   +   C  T+ D       L EG  Y+FR+ A N AG  S PS  T+  +C+ +   P I  D       +++   + + + I + G+P P+ SW K G  +  SD   I +       ++ML +  +   DAG YT+ A N  G+    + + VL
Sbjct: 21221 WTEPKYDGGHKLTGYIVEKRDLPSKSWMKANHVNVPECAFTVTD-------LVEGGKYEFRIRAKNTAGAISAPSESTETIICKDEYEAPTIVLDPTIKDGLTIKAGDTIVLNAISILGKPLPKSSWSKAGKDIRPSDITQITSTP----TSSMLTIKYATRKDAGEYTITATNPFGTKVEHVKVTVL 21397

HSP 13 Score: 73.1738 bits (178), Expect = 2.905e-12
Identity = 56/188 (29.79%), Postives = 89/188 (47.34%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIK--VKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W KP+ DGG  IT YI++K+D   G W  A  T+       + + +  V GL+E + Y+FRV+A N AG  S PS  T P   + +   P I        ++ V+    + ++  V G+P P   W +   ++  S    I   +++    A+L V  +   D G Y L A N  GS    +++ VL
Sbjct: 24467 QWTKPVYDGGSMITGYIVEKRDLPDGRWMKASFTN-------VIETQFTVSGLTEDQRYEFRVIAKNAAGAISKPSDSTGPITAKDEVELPRISMDPKFRDTIVVNAGETFRLEADVHGKPLPTIEWLRGDKEIEESARCEIKNTDFK----ALLIVKDAIRIDGGQYILRASNVAGSKSFPVNVKVL 24643

HSP 14 Score: 71.2478 bits (173), Expect = 1.157e-11
Identity = 56/185 (30.27%), Postives = 78/185 (42.16%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSK--SVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W +P  DGG  I  Y ++     G   D  V  + A    I   E    GL E   YQFR +A      SPPS  + P     +N+ P ID   A     +V+   +      V G+P P  SW K+G  L  ++   +A           L++F     D+G YT+ AEN +GS    + L VL
Sbjct: 20137 WARPKHDGGSKIIGYFVEACKLPG---DKWVRCNTA-PHQIPQEEYTATGLEEKAQYQFRAIARTAVNISPPSEPSDPVTILAENVPPRIDLSVAMKSLLTVKAGTNVCLDATVFGKPMPTVSWKKDGTLLKPAEGIKMAMQR----NLCTLELFSVNRKDSGDYTITAENSSGSKSATIKLKVL 20313

HSP 15 Score: 70.8626 bits (172), Expect = 1.261e-11
Identity = 52/175 (29.71%), Postives = 77/175 (44.00%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPC---IDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNG 417
            W KPL DGG PI  Y+++ +      ++ +  D+      I     +VPGL EG  Y+FR+ AAN  G+  P  E    +     L P    +D       +VRV ++     +VKG P P  +W K G  L+         D  Q      LQ+  +   D G Y + A+N +G
Sbjct: 18751 WTKPLSDGGSPILGYVVECQKPGTAQWNRINKDE-----LIRQCAFRVPGLIEGNEYRFRIKAANIVGEGEP-RELAESVIAKDILHPPEVELDVTCRDVITVRVGQTIRILARVKGRPEPDITWTKEGKVLVREKR----VDLIQDLPRVELQIKEAVRADHGKYIISAKNSSG 18915

HSP 16 Score: 70.4774 bits (171), Expect = 1.709e-11
Identity = 56/189 (29.63%), Postives = 83/189 (43.92%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSS-----ETKPHLCRHKNLRPCIDKGAAGSK-SVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  PL DGG  I  YI   ++      +    D     + + DL   V GL EGK Y+FRV A N  G S P       E K  L   K L P      AG K  +  H        V G+P P   W K  ++++ S    +    ++  ++++L +      D+G Y+L AEN +G+D   + +VV+
Sbjct: 19746 WLPPLRDGGAKIDGYITSYRE------EEQPADRWTEYSVVKDLSLVVTGLKEGKKYKFRVAARNAVGVSLPREAEGVYEAKEQLLPPKILMPEQITIKAGKKLRIEAH--------VYGKPHPTCKWKKGEDEVVTSSHLAV----HKADSSSILIIKDVTRKDSGYYSLTAENSSGTDTQKIKVVVM 19916

HSP 17 Score: 70.0922 bits (170), Expect = 2.418e-11
Identity = 55/188 (29.26%), Postives = 83/188 (44.15%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVH--RSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+KP  DGG  IT YI++KKD   G W  A  T+       + + E  V GL E + Y+FRV+A N AG+ S PS  +     R +   P           + VH   + +    ++G+P P   W K+G +L  +   +      Q     +    R+   D G Y L   N  G+  I + + VL
Sbjct: 25549 KWKKPAYDGGSKITGYIVEKKDLPDGRWMKASFTN-------VLETEFTVSGLVEDQRYEFRVIARNAAGNFSEPSDSSGAITARDEIDAPNASLDPKYKDVIVVHAGETFVLEADIRGKPIPDVVWSKDGKELEETAARMEIKSTIQKTTLVVKDCIRT---DGGQYILKLSNVGGTKSIPITVKVL 25726

HSP 18 Score: 69.707 bits (169), Expect = 3.219e-11
Identity = 55/183 (30.05%), Postives = 80/183 (43.72%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDK-FGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETK----------PHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNG 417
            W KP+ DGG  I  Y+++ ++K    W+           ATI + E  VP L  G+ Y FRV A N  G S  S              P L    +L+  +        ++R   S    + V G PPP  +W K G  L  S   +  T+ Y     ++L V +   +DAG YT+ AEN++G
Sbjct: 30289 WTKPMYDGGTDIVGYVLEMQEKDTDQWYRV------HTNATIRNTEFTVPDLKMGQKYSFRVAAVNVKGMSEYSESIAEIEPVERIEIPDLELADDLKKTV--------TIRAGASLRLMVSVSGRPPPVITWSKQGIDLA-SRAIIDTTESY-----SLLIVDKVNRYDAGKYTIEAENQSG 30451

HSP 19 Score: 69.707 bits (169), Expect = 3.451e-11
Identity = 58/188 (30.85%), Postives = 81/188 (43.09%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQK-KDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGA-AGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            +WE PL DGG PI  YII++ ++    W        +     + +L  KV GL +G  Y +RV A NKAG S PS    P       + P +D  A      V V       +   G P P  +W      L   D   + T      A A L +  S+  D G YTL  ENR  +   ++D+ V+A+
Sbjct: 14238 KWEPPLKDGGSPILGYIIERCEEGKDNWI-------RCNMKLVPELTYKVTGLEKGNKYLYRVSAENKAGVSDPSEILGPLTADDAFVEPTMDLSAFKDGLEVIVPNPITILVPSTGYPRPTATWCFGDKVLETGDRVKMKTLS----AYAELVISPSERSDKGIYTLKLENRVKTISGEIDVNVIAR 14414

HSP 20 Score: 69.707 bits (169), Expect = 3.481e-11
Identity = 54/188 (28.72%), Postives = 88/188 (46.81%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG--WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP---CIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W+ P  DGG  I  YI++ + K G   W  A  T + +C  T    + KV GL +G+ Y+FRV+A N AG+S P+   +P L + + L P    +D   A  + ++V  +      +KG P P+ +W K            +          + L++  +   D G Y+L  EN  GS  + + ++VL
Sbjct: 18060 WQPPRHDGGSKILGYIVEYQ-KVGDEEWRRANHTPE-SCPET----KYKVTGLRDGQTYKFRVLAVNAAGESDPAHVPEPVLVKDR-LEPPELILDANMAREQHIKVGDTLRLSAIIKGVPFPKVTWKKEDRDAPTKARIDVTP------VGSKLEIRNAAHEDGGIYSLTVENPAGSKTVSVKVLVL 18234

HSP 21 Score: 69.3218 bits (168), Expect = 4.554e-11
Identity = 52/172 (30.23%), Postives = 82/172 (47.67%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSV--RVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATA-MLQVFRSQMHDAGTYTLVAEN 414
            W KP  DGG  IT Y+I+ + K         +D      T+  LE  V  L+EG+ Y F+V+A N AG S P  E++P + + + + P +D      K V  +   +    I V G P P  +W K G++++   + V     ++  AT+ +L +      D+G Y L A N
Sbjct: 21912 WPKPKHDGGSKITGYVIEAQRKG--------SDQWTHITTVKGLECVVRNLTEGEEYTFQVMAVNSAGRSAP-RESRPVIVKEQTMLPELDLRGIYQKLVIAKAGDNIKVEIPVLGRPKPTVTW-KKGDQILKQTQRV----NFETTATSTILNINECVRSDSGPYPLTARN 22069

HSP 22 Score: 68.9366 bits (167), Expect = 5.905e-11
Identity = 54/189 (28.57%), Postives = 85/189 (44.97%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQ-KKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPP---SSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            +WEKP  DGG+ I  Y ++ ++     W        K+    I D +  + GL E   Y+FRV A N+ G S P   +   K  L   +   P I K      + ++  +A    ++ G P P   WY+ G +LI S ++ +++D    G T  L V   +  D G YT +A N  G  +    L++ A
Sbjct: 31377 QWEKPECDGGKEILGYWVEYRQSGDSAW-------KKSNKERIKDKQFTIGGLLEATEYEFRVFAENETGLSRPRRTAMSIKTKLTSGE--APGIRK-EMKDVTTKLGEAAQLSCQIVGRPLPDIKWYRFGKELIQSRKYKMSSD----GRTHTLTVMTEEQEDEGVYTCIATNEVGEVETSSKLLLQA 31551

HSP 23 Score: 65.855 bits (159), Expect = 4.712e-10
Identity = 53/186 (28.49%), Postives = 80/186 (43.01%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQ-KKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKS--VRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG PI  Y+++ K+     W    +  +      +     +V GL E   YQFRV A NK G S PS     H  +   + P  +  A   K+  +R   +   ++ VKG PPP+ +W K    L +     I + ++       L+      +DAG Y L  EN  G  +  + + VL
Sbjct: 20536 WGKPAYDGGSPIIGYLVEVKRADSDNWVRCNLPQN------LQKTRFEVTGLMEDTQYQFRVYAVNKIGYSDPSDVPDKHYPKDILIPPEGELDADLRKTLILRAGVTMRLYVPVKGRPPPKITWSKPNVNLRDRIGLDIKSTDFD----TFLRCENVNKYDAGKYILTLENSCGKKEYTIVVKVL 20711

HSP 24 Score: 65.0846 bits (157), Expect = 7.981e-10
Identity = 61/187 (32.62%), Postives = 80/187 (42.78%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQK-KDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAG--DSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYK-NGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP+ DGG  I  YII+  K   G W      + +AC  T       V  L  G+ Y+FRV A N AG  DS   + T   + R       ID     +  VR   S    I  +G P P   W K + N  + +D  +  TD +     + L V     +DAG YTL  EN +GS  I   + VL
Sbjct: 29201 WSKPVFDGGMEIIGYIIEMCKADLGDWHK---VNAEACVKT----RYTVTDLQAGEEYKFRVSAINGAGKGDSCEVTGTIKAVDRLTAPELDIDANFKQTHVVRAGASIRLFIAYQGRPTPTAVWSKPDSNLSLRAD--IHTTDSF-----STLTVENCNRNDAGKYTLTVENNSGSKSITFTVKVL 29373

HSP 25 Score: 64.3142 bits (155), Expect = 1.461e-9
Identity = 61/193 (31.61%), Postives = 88/193 (45.60%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKF----GGWFDALVTDDKACTATI-DDLEAKVPGLSEGKWYQFRVVAANKAG-DSPPSSETKPHLCRHKNLRPCIDKGA---AGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGG-ATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG  +  YII++K       G W          C  TI  D    V  LSEG  Y+FRV+A N AG  S  S  T P  CR +   P  +  A       ++R     +    V G+P P+  W K G+K ++  E V  + +Y G  ATA+++       D+G YTL  +N +G+  + + + VL
Sbjct: 29886 WSAPAYDGGSKVVGYIIERKPVSEVGDGRWLK--------CNYTIVSDNFFTVTALSEGDTYEFRVLAKNAAGVISKGSESTGPVTCRDEYAPPKAELDARLHGDLVTIRAGSDLVLDAAVGGKPEPKIIWTK-GDKELDLCEKV--SLQYTGKRATAVIKF--CDRSDSGKYTLTVKNASGTKAVSVMVKVL 30065

HSP 26 Score: 63.929 bits (154), Expect = 1.844e-9
Identity = 57/184 (30.98%), Postives = 82/184 (44.57%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPS---------SETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLIN-SDEFVIATDEY-QGGATAMLQVFRSQMHDAGTYTLVAENRNGS 418
            W++P  +GG PI  YII+K+      F+ +  + + C  T       V  L E + Y+FRV A N+ G+S PS          E  P +    ++R    K  AG     VH  A     V G P P+  W KN   +   +D   I  +E  +  A   L + ++   D GTYT+ A NR GS
Sbjct: 16242 WKEPRSNGGSPIQGYIIEKRRHDKPDFERV--NKRLCPTT----SFLVENLDEHQMYEFRVKAVNEIGESEPSLPLNVVIQDDEVPPTIKLRLSVRGDTIKVKAGEP---VHIPA----DVTGLPMPKIEWSKNETVIEKPTDALQITKEEVSRSEAKTELSIPKAVREDKGTYTVTASNRLGS 16412

HSP 27 Score: 63.929 bits (154), Expect = 2.154e-9
Identity = 34/89 (38.20%), Postives = 48/89 (53.93%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W++P+DDGG  IT Y +++++K    W  A+ T        + DL  KV GL EG  Y+FRV A N+AG  PPS  +   L +     P
Sbjct: 21519 WKRPVDDGGSEITGYHVERREKKSLRWVRAIKT-------PVSDLRCKVTGLQEGSTYEFRVSAENRAGIGPPSEASDSVLMKDAAYPP 21600

HSP 28 Score: 63.5438 bits (153), Expect = 2.452e-9
Identity = 53/177 (29.94%), Postives = 79/177 (44.63%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHI--KVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDK 420
            W  PL DGG  +T YI++ K++     +     DK    T    +  V GL EG +Y+FRV A N AG   P   T     + + + P +   A+    + VH   +  I   V G+PPP  +W  N   L        AT E    +++M+ +   Q    G Y+L+A+N  G  K
Sbjct: 17368 WSPPLKDGGSKVTGYIVEYKEEGKE--EWEKGKDKEVRGT----KLVVTGLKEGAFYKFRVRAVNIAGIGEPGEVTDVIEMKDRLVSPDLQLDASVRDRIVVHAGGVIRIIAYVSGKPPPTVTWNMNERTLPQE-----ATIETTAISSSMV-IKNCQRSHQGVYSLLAKNEAGERK 17532

HSP 29 Score: 63.1586 bits (152), Expect = 3.203e-9
Identity = 52/189 (27.51%), Postives = 86/189 (45.50%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQK----KDKFGGWFDALVTDDKACTATIDDLEAKVP-GLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            W+ P  +GG PI  Y ++      +K   W        +  +  I DL+ KV  G+   K Y  RV A N  G S PS  ++  + +  + +P ID        +   + +I  +  +  P P  SW+K+G ++  SD   +  D      +A L+V +S   DAG YT+  EN+ GS    +++ V+ 
Sbjct: 15232 WKPPEKNGGSPIIGYHVEMCPVGTEK---WM-------RVNSRPIKDLKFKVEEGVVPDKEYVLRVRAVNAIGVSEPSEISENVVAKDPDCKPTIDLETHDIIVIEGEKLSI-PVPFRAVPVPTVSWHKDGKEVKASDRLTMKNDHI----SAHLEVPKSVRADAGIYTITLENKLGSATASINVKVIG 15405

HSP 30 Score: 63.1586 bits (152), Expect = 3.492e-9
Identity = 51/187 (27.27%), Postives = 82/187 (43.85%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDK--ACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCI--DKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W+ P +DGG  I  YI+++  +           DK  AC   + + + +V GL EGKWY +RV A N+ G S PS  T+          P I  D       +V+          V G+P P+ +W K  + ++  D+ +   +  +     ++   RS   D GTY + A N  G     +++ VL
Sbjct: 14535 WDPPKNDGGSRIKGYIVERCPR---------GSDKWVACGEPVAETKMEVTGLEEGKWYAYRVKALNRQGASKPSRPTEEIQAVDTQEAPEIFLDVKLLAGLTVKAGTKIELPATVTGKPEPKITWTK-ADMILKQDKRITIENVPKKSTVTIVDSKRS---DTGTYIIEAVNVCGRATAVVEVNVL 14708

HSP 31 Score: 62.7734 bits (151), Expect = 4.846e-9
Identity = 33/76 (43.42%), Postives = 43/76 (56.58%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSET 321
            W +P+DDGG  I  YI++K+DK G      V   K    T+ DL  +V GL+EG  Y+FRV A N AG   PS  +
Sbjct: 25849 WARPVDDGGTEIEGYILEKRDKEG------VRWTKCNKKTLTDLRLRVTGLTEGHSYEFRVAAENAAGVGEPSEPS 25918

HSP 32 Score: 61.2326 bits (147), Expect = 1.375e-8
Identity = 58/219 (26.48%), Postives = 92/219 (42.01%), Query Frame = 0 
Query:  216 GLLGQLGEELLAEEHVLSRLQLLLPMHLGR------WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            GL  +  E + A E  L   ++ L M + R      WEKP  DGG  I  YI++ + K         +D  A  AT+   EA + GL +G+ Y FRV A N+ G S P   + P + +   + P   K    + +V         +   G P P  +W+K+   L  +      + E      ++L +  +   D G Y +   N  G     L+++VL
Sbjct: 22961 GLPAETAESVKASERPLPPGKITL-MDVTRNSVSLSWEKPEHDGGSRILGYIVEMQTKG--------SDKWATCATVKVTEATITGLIQGEEYSFRVSAQNEKGISDPRQLSVPVIAKDLVIPPAF-KLLFNTFTVLAGEDLKVDVPFIGRPTPAVTWHKDNVPLKQTTRVNAESTE----NNSLLTIKDACREDVGHYVVKLTNSAGEAIETLNVIVL 23165

HSP 33 Score: 61.2326 bits (147), Expect = 1.460e-8
Identity = 41/133 (30.83%), Postives = 60/133 (45.11%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSV--RVHRSAIWHIKVKGEPPPQFSWYKNGNKL 374
            W++P  DGG  IT YI++++D   G W        KA    + + +  + GL++   Y+FRV A N  G  S PS    P  C      P ID     ++ V  R   S      + G+P P   WYK+  +L
Sbjct: 27715 WQQPAFDGGSKITGYIVERRDLPDGRW-------TKASFTNVTETQFIISGLTQNSQYEFRVFARNAVGSISNPSEVVGPITCIDSYGGPVIDLPLEYTEVVKYRAGTSVKLRAGISGKPAPTIEWYKDDKEL 27840

HSP 34 Score: 60.8474 bits (146), Expect = 1.842e-8
Identity = 50/181 (27.62%), Postives = 81/181 (44.75%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCID--------KGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGS 418
            W+ P  DGG  I +Y ++K++K    + ++ T++      I +    V  L EG  Y+FRV   N  G+S  S  ++P       + P  D        K    + +VR   +A    KV G P P   WY+ G ++I +D       E++GG   ++ +      DA  Y + A N+ GS
Sbjct: 31772 WKPPASDGGAKIRNYYLEKREKKQNKWISVTTEE------IRETVFSVKNLIEGLEYEFRVKCENLGGESEWSEISEP-------ITPKSDVPIQAPHFKEELRNLNVRYQSNATLVCKVTGHPKPIVKWYRQGKEII-ADGLKYRIQEFKGGYHQLI-IASVTDDDATVYQVRATNQGGS 31937

HSP 35 Score: 60.8474 bits (146), Expect = 2.008e-8
Identity = 56/189 (29.63%), Postives = 80/189 (42.33%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKF-GGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWH--IKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATA---MLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG  I HY+++  +K    W    V                V GL E   Y FRV A N+AG S P     P L + +   P ID     S +V V   +     I + G+P P+ +  ++G  L        AT  +    TA    + +  S   DAG Y + A N +G+ K  +++VVL
Sbjct: 27322 WLKPEHDGGSRIVHYVVEALEKGQKNWVKCAVAKST---------HHVVSGLRENSEYFFRVFAENQAGLSDPRELLLPVLIKEQLEPPEIDMKNFPSHTVYVRAGSNLKVDIPISGKPLPKVTLSRDGVPL-------KATMRFNTEITAENLTINLKESVTADAGRYEITAANSSGTTKAFINIVVL 27494

HSP 36 Score: 60.8474 bits (146), Expect = 2.133e-8
Identity = 32/78 (41.03%), Postives = 42/78 (53.85%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETK 322
            W +P+ DGG  I+ Y ++K+DK   GWF       K    TI D   KV GL+E   YQ+RV A N AG  P S  ++
Sbjct: 28013 WSRPIADGGSDISGYFLEKRDKKSLGWF-------KVLKETIRDTRQKVTGLTENSDYQYRVCAVNAAGQGPFSEPSE 28083

HSP 37 Score: 60.077 bits (144), Expect = 3.010e-8
Identity = 33/83 (39.76%), Postives = 43/83 (51.81%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKP-HLCR 327
            W  P  DGG PIT Y +++++  G W        +     I DL+ +V GL EG  Y+FRV A N AG S PS  + P   CR
Sbjct: 18651 WTLPKSDGGSPITGYYMERREVTGKWV-------RVNKTPIADLKFRVTGLYEGNTYEFRVFAENLAGLSKPSPSSDPIKACR 18726

HSP 38 Score: 60.077 bits (144), Expect = 3.062e-8
Identity = 31/80 (38.75%), Postives = 45/80 (56.25%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHL 325
            W  PLD+GG PIT Y ++K+++ G  + + V+        +  +E  VP L EG  YQFR +A N AG  PPS  + P +
Sbjct: 16546 WTPPLDNGGSPITGYWLEKREE-GSPYWSRVSRAPITKVGLKGVEFNVPRLLEGVKYQFRAMAINAAGIGPPSEPSDPEV 16624

HSP 39 Score: 60.077 bits (144), Expect = 3.142e-8
Identity = 32/74 (43.24%), Postives = 42/74 (56.76%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPS 318
            W  P  DGG  I +YI++++DK G  W        K    T+ DL  KV GL+EG  Y+FR++A N AG S PS
Sbjct: 22602 WGHPDSDGGSEIINYIVERRDKAGQRWI-------KCNKKTLTDLRYKVSGLTEGHEYEFRIMAENAAGISAPS 22668

HSP 40 Score: 60.077 bits (144), Expect = 3.637e-8
Identity = 48/187 (25.67%), Postives = 80/187 (42.78%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIK--VKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            W+KP  DGG  I  Y++    +   W   +    K+ +     L+     L+EGK Y FRV A N+ G+  PS  T   + R   + P +D             ++ + +K  +KG+P P  SW K  + L       + +          L V+  Q  DAG YT+  +N  G+ +  + + V+ +
Sbjct: 20831 WKKPHSDGGSRIIGYVVDFLTEENKWQRVM----KSLS-----LQYSAKDLTEGKEYTFRVSAENENGEGTPSEITV--VARDDVVAPDLDLKGLPDLCYLAKENSNFRLKIPIKGKPAPSVSWKKGEDPLATDTRVSVESS----AVNTTLIVYDCQKSDAGKYTITLKNVAGTKEGTISIKVVGK 21002

HSP 41 Score: 60.077 bits (144), Expect = 3.668e-8
Identity = 49/177 (27.68%), Postives = 73/177 (41.24%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSV--RVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDK 420
            W KP+ DGG  +T Y+++ +      +  + T  +  T      E  V  L  G  Y FRV A N AG   P    +P   +     P ID   A   SV  +        I  KG PPP  +W K+   L +   + I   +    ++++L + +   +D G Y L  EN  G  K
Sbjct: 28114 WSKPVYDGGSAVTGYVVEIRQGEEEEWTTVSTKGEVRTT-----EYVVSNLKPGVNYYFRVSAVNCAGQGEPIEMNEPVQAKDILEAPEIDLDVALRTSVIAKAGEDVQVLIPFKGRPPPTVTWRKDEKNLGSDARYSIENTD----SSSLLTIPQVTRNDTGKYILTIENGVGEPK 28281

HSP 42 Score: 60.077 bits (144), Expect = 3.700e-8
Identity = 48/187 (25.67%), Postives = 79/187 (42.25%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACT--ATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWH--IKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP+ DGG P+  Y+++ K       +A   +   CT    +   +  V  L E   Y FR+ A N  G   P++     + + +   P I+  A   K V +  SA     + +KG P P+  W K    L +  +  + +      +  ML +      D+G Y L  EN +GS    +++ VL
Sbjct: 25950 WSKPIYDGGAPVKGYVVEVK-------EAAADEWTTCTPPTGLQGKQFTVTKLKENTEYNFRICAINSEGVGEPATLPGSVVAQERIEPPEIELDADLRKVVVLRASATLRLFVTIKGRPEPEVKWEKAEGILTDRAQIEVTS------SFTMLVIDNVTRFDSGRYNLTLENNSGSKTAFVNVRVL 26123

HSP 43 Score: 59.6918 bits (143), Expect = 3.896e-8
Identity = 50/178 (28.09%), Postives = 78/178 (43.82%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETK--PHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNG--NKLINSDEFVIATDEYQGGA--TAMLQVFRSQMHDAGTYTLVAENRNG 417
            W+ P  DGG PI  YI++ +++    +  +   DK  T      E  VP L E + Y+FRV A N+AG+S PS  T   P     +     ID GA      +          +KG P P+ SW  +G   K +      I  D     A  ++++ +   +    G Y++ A+N+ G
Sbjct: 16652 WKPPAKDGGSPIKGYIVEMQEEGTTDWKRVNEPDKLITTC----ECVVPNLKELRKYRFRVKAVNEAGESEPSDTTGEIPATDIQEEPEVFIDIGAQDCLVCKAGSQIRIPAVIKGRPTPKSSWEFDGKAKKAMKDGVHDIPEDAQLETAENSSVIIIPECKRSHTGKYSITAKNKAG 16825

HSP 44 Score: 58.9214 bits (141), Expect = 7.238e-8
Identity = 52/189 (27.51%), Postives = 83/189 (43.92%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGD-SPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIK--VKGEPPPQFSWYKNGNKLIN-SDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W KP  DGG  IT YI+++++   G W     T+       I +   +V  L+E + Y+FRV A N A   S PS  T P + +     P +         + V    +  I   + G P P  SW K+G ++   +   +I+TD +      +L V      D G Y L  +N  G+  + ++  VL
Sbjct: 26631 KWSKPHYDGGAKITGYIVERRELPDGRWLKCNYTN-------IQETYFEVTELTEDQRYEFRVFARNAADSVSEPSESTGPIIVKDDVEPPRVMMDVKFRDVIVVKAGEVLKINADIAGRPLPVISWAKDGIEIEERARTEIISTDNH-----TLLTVKDCIRRDTGQYVLTLKNVAGTRSVAVNCKVL 26807

HSP 45 Score: 58.9214 bits (141), Expect = 8.450e-8
Identity = 33/88 (37.50%), Postives = 42/88 (47.73%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W+ P DDGG PIT Y+++K++         V   K     I +   K  GL EG  Y+FRV A NKAG   PS  +K    R     P
Sbjct: 20435 WKPPADDGGSPITGYLLEKRET------QAVNWTKVNRKPIIERTLKATGLQEGTEYEFRVTAINKAGPGKPSDASKAAYARDPQYPP 20516

HSP 46 Score: 58.5362 bits (140), Expect = 1.075e-7
Identity = 33/78 (42.31%), Postives = 41/78 (52.56%), Query Frame = 0 
Query:  241 MHLGRWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPP 317
            M L  W  P DDGG  IT +II++KD K   W   + T+   C  T         GL EG+ Y+FRV+A NK G  PP
Sbjct: 16139 MCLLNWSDPEDDGGSEITGFIIERKDAKMHTWRQPIETERSKCDIT---------GLLEGQEYKFRVIAKNKFGCGPP 16207

HSP 47 Score: 58.5362 bits (140), Expect = 1.103e-7
Identity = 37/89 (41.57%), Postives = 46/89 (51.69%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTAT-IDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W KP  DGG  I  Y+I+   K G       TD+    A  +   +  +PGL EG+ Y FRV A NKAG+S PS  + P LCR K   P
Sbjct: 14931 WTKPEHDGGAKIESYVIEML-KTG-------TDEWVRVAEGVPTTQHLLPGLMEGQEYSFRVRAVNKAGESEPSEPSDPVLCREKLYPP 15011

HSP 48 Score: 58.151 bits (139), Expect = 1.244e-7
Identity = 52/178 (29.21%), Postives = 73/178 (41.01%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACT--ATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSK--SVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGS 418
            W KP+ DGG  I  YI++K D   G W          CT    I+    +V  L E   Y FR+ A NKAG    +    P +   K   P ID      K  ++R   S    + +KG P P+  W K   + I     +  T  +       L +     +D+G YTL  EN +G+
Sbjct: 23785 WSKPIYDGGCEIQGYIVEKCDVSVGEW--------TMCTPPTGINKTNIEVEKLLEKHEYNFRICAINKAGVGEHADVPGPIIVEEKLEAPDIDLDLELRKIINIRAGGSLRLFVPIKGRPTPEVKWGKVDGE-IRDAAIIDVTSSFTS-----LVLDNVNRYDSGKYTLTLENSSGT 23948

HSP 49 Score: 57.7658 bits (138), Expect = 1.638e-7
Identity = 48/172 (27.91%), Postives = 76/172 (44.19%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKF-GGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKG--AAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAEN 414
            W KP  DGG  IT Y++++K K    W  A ++  K C       E +V  L E    +FRV A N+ G S P +   P   +   + P +D         +VR+  +    +  KG+P P  SW K+G  L  S+    +  E +      L +  ++    G YT++ +N
Sbjct: 28410 WTKPDFDGGSVITEYVVERKGKGEQTWSHAGIS--KTC-------EIEVSQLKEQSVLEFRVFAKNEKGLSDPVT-IGPITVKELIITPEVDLSDIPGAQVTVRIGHNVHLELPYKGKPKPSISWLKDGLPLKESEFVRFSKTENK----ITLSIKNAKKEHGGKYTVILDN 28567

HSP 50 Score: 57.3806 bits (137), Expect = 2.330e-7
Identity = 57/194 (29.38%), Postives = 82/194 (42.27%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG------GWFDALVTDDKACTATIDDL--EAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPC-IDKGAAGSKSVRVHRSAIWHI--KVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP+ DGG PI  Y+++ + K        GW        K C A    +  E  V  L E + Y+FRV A N+ G   P +E K  +   + L P  ID  A+  K V V       +   V+G P P+ +W K G   +     V   D       A L +  S   D+G Y+L   N  G   + +++ VL
Sbjct: 19444 WGKPVYDGGAPIIGYVVEMRPKIADASPDEGW--------KRCNAAAQLVRKEFTVTSLDENQEYEFRVCAQNQVGIGRP-AELKEAIKPKEILEPPEIDLDASMRKLVIVRAGCPIRLFAIVRGRPAPKVTWRKVGIDNVVRKGQVDLVD-----TMAFLVIPNSTRDDSGKYSLTLVNPAGEKAVFVNVRVL 19623

HSP 51 Score: 57.3806 bits (137), Expect = 2.453e-7
Identity = 53/174 (30.46%), Postives = 70/174 (40.23%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTA-TIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHR--SAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENR 415
            W+ PL DGG  I  YII+K  K    W        K C    +  L     GL EGK YQFRV A N AG S PS  T P     K + P          S+ V R         + G P P  +W K        DE VI  +E +  A  +++  + ++ +   + L    R
Sbjct: 15527 WKPPLYDGGSKIMGYIIEKIAKGEERW--------KRCNEHLVPILTYTAKGLEEGKEYQFRVRAENAAGISEPSRATPP----TKAVDPIDAPKVILRTSLEVKRGDEIALDASISGSPYPTITWIK--------DENVIVPEEIKKRAAPLVRRRKGEVQEEEPFVLPLTQR 15680

HSP 52 Score: 56.9954 bits (136), Expect = 2.650e-7
Identity = 31/81 (38.27%), Postives = 42/81 (51.85%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATID---DLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKP 323
            WE P DDGG P+T Y+++K++             K  T  +D   DLE  VP L +GK Y F+V A NK G   P+   +P
Sbjct: 14435 WEPPDDDGGSPLTGYVVEKRE----------VSRKTWTKVMDFVTDLEFTVPDLVQGKEYLFKVCARNKCGPGEPAYVDEP 14505

HSP 53 Score: 56.9954 bits (136), Expect = 2.815e-7
Identity = 31/77 (40.26%), Postives = 44/77 (57.14%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETK 322
            W++P  DGG PI  Y+I++K++     ++L+   KA    +   E    GL EG  Y FR+ A NKAG SPPS  T+
Sbjct: 20039 WDRPDSDGGSPIIGYLIERKER-----NSLLWV-KANDTLVRSTEYPCAGLVEGLEYSFRIYALNKAGSSPPSKPTE 20109

HSP 54 Score: 56.6102 bits (135), Expect = 3.868e-7
Identity = 32/75 (42.67%), Postives = 42/75 (56.00%), Query Frame = 0 
Query:  243 LGRWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPP 317
            L +WE PLDDGG  I +Y ++KKDK     + +V      T+T+   +  V  L EGK Y FRV A N+ G  PP
Sbjct: 15746 LCKWEPPLDDGGSEIINYTLEKKDKTKPDSEWIVV-----TSTLRHCKYSVTKLIEGKEYLFRVRAENRFGPGPP 15815

HSP 55 Score: 56.225 bits (134), Expect = 4.753e-7
Identity = 31/79 (39.24%), Postives = 42/79 (53.16%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETK 322
            +WE P +DGGRPI  Y+I+KK++ G  W  A  T    C   + D       + EG   QF+V A N+AG   PS  T+
Sbjct: 15130 KWEPPKNDGGRPIQRYVIEKKERLGTRWVKAGKTAGPDCNFRVTD-------VIEGTEVQFQVRAENEAGVGHPSEPTE 15201

HSP 56 Score: 56.225 bits (134), Expect = 5.269e-7
Identity = 32/89 (35.96%), Postives = 45/89 (50.56%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            +W KP  DGG  I++Y++ K++K    +  +  D       + D   KV  L EG  YQFRV A N AG+S PS  +    CR  +  P
Sbjct: 30186 QWTKPESDGGNEISNYLVDKREKKSLRWTRVNKD-----YVVYDTRLKVTSLMEGCDYQFRVTAVNAAGNSEPSEASNFISCREPSYTP 30269

HSP 57 Score: 55.8398 bits (133), Expect = 6.530e-7
Identity = 34/92 (36.96%), Postives = 50/92 (54.35%), Query Frame = 0 
Query:  240 PMHLG-RWEKPLDDGGRPITHYIIQKKD-KFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHK 329
            P H+   W+ P DDGG P+ +YI++K+D +   W   +V+   + T      + K+P L +G  Y FRV A NK G  PP   T P + +HK
Sbjct: 18545 PEHMTISWQPPKDDGGSPVINYIVEKQDTRKDTW--GVVSSGSSKT------KLKIPHLQKGCEYVFRVRAENKIGVGPPLDST-PTVAKHK 18627

HSP 58 Score: 55.8398 bits (133), Expect = 7.116e-7
Identity = 52/188 (27.66%), Postives = 78/188 (41.49%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKV--KGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            W KP  DGG  IT Y+++ + K    W +A  T  K  T T++ L  K         Y+FRV A N AG S P       + +   + P  D     ++ +     + + I V   G P P+ +W     +L  +D   I T +        L V  S   D+G Y L  EN  G     + +VV+ +
Sbjct: 29493 WLKPDHDGGSRITGYLLEMRQKGSDFWVEAGHT--KQLTFTVERLVEKTE-------YEFRVKAKNDAGYSEPREAFSSVIIKEPQIEPTADLTGITNQLITCKAGSPFTIDVPISGRPAPKVTWKLEEMRLKETDRVSITTTK----DRTTLTVKDSMRGDSGRYFLTLENTAGVKTFSVTVVVIGR 29667

HSP 59 Score: 55.8398 bits (133), Expect = 7.492e-7
Identity = 31/78 (39.74%), Postives = 42/78 (53.85%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKP 323
            W+ PL DGG  IT+YI++K++     +    T    CT        KV GLSEG  Y FRV+A N+ G   P+  T+P
Sbjct: 21815 WDLPLIDGGSRITNYIVEKREATRKSYSTATTKCHKCTY-------KVTGLSEGCEYFFRVMAENEYGIGEPTETTEP 21885

HSP 60 Score: 55.4546 bits (132), Expect = 8.305e-7
Identity = 31/76 (40.79%), Postives = 41/76 (53.95%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSET 321
            WE P  DGG  IT+Y+I+ +DK        +  D A T   +DL A V  + EG+ Y FRV A N+ G   PS+ T
Sbjct: 14138 WEPPEYDGGAEITNYVIELRDKTS------IRWDTAMTVRAEDLSATVTDVVEGQEYSFRVRAQNRIGVGKPSAAT 14207

HSP 61 Score: 55.4546 bits (132), Expect = 8.305e-7
Identity = 35/89 (39.33%), Postives = 48/89 (53.93%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W KP  DGG+PIT+YI++K++     W  A VT D     T    + +VP L EG  Y+FRV A N+ G   PS  +KP   +    +P
Sbjct: 17959 WNKP-HDGGKPITNYILEKRETMSKRW--ARVTKDPIHPYT----KFRVPDLLEGCQYEFRVSAENEIGIGDPSPPSKPVFAKDPIAKP 18040

HSP 62 Score: 55.4546 bits (132), Expect = 9.365e-7
Identity = 48/189 (25.40%), Postives = 82/189 (43.39%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQ----KKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWH--IKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP+ DGG  IT Y+++    ++D++      +VT      AT       + GL+E + Y+ R+ A N  G   P+          + L P I+  A   K V +         + +KG P P+  W ++  + ++    + +T  Y      +L V      D+G Y L  EN +GS    +++ VL
Sbjct: 22703 WNKPIYDGGSEITGYMVEIALPEEDEW-----QIVTPPAGLKAT----SYTITGLTENQEYKIRIYAMNSEGLGEPALVPGTPKAEDRMLPPEIELDADLRKVVTIRACCTLRLFVPIKGRPAPEVKWARDHGESLDKAS-IESTSSY-----TLLIVGNVNRFDSGKYILTVENSSGSKSAFVNVRVL 22876
[Close]
BLAST of low quality protein: twitchin-like vs. SwissProt
Match: gi|296439237|sp|Q14324.2|MYPC2_HUMAN (RecName: Full=Myosin-binding protein C, fast-type; Short=Fast MyBP-C; AltName: Full=C-protein, skeletal muscle fast isoform)

HSP 1 Score: 64.3142 bits (155), Expect = 1.431e-9
Identity = 63/196 (32.14%), Postives = 80/196 (40.82%), Query Frame = 0 
Query:  243 LGRWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAG--DSPPSS----------------ETKPHLCRH--KNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNG 417
            L  W+ P DDG   I  Y +QK DK    WF+ +   ++  + T+ DL         G  Y FRV   N  G  DSP  S                E K H  R   K L P ID+            SA  +  V+G P P+  W KN  ++    +F+I    YQG  T  L + R    DAGTYT  A N  G
Sbjct:  950 LVEWQAPKDDGNSEIMGYFVQKADKKTMEWFN-VYERNRHTSCTVSDLIV-------GNEYYFRVYTENICGLSDSPGVSKNTARILKTGITFKPFEYKEHDFRMAPKFLTPLIDRVVVAG------YSAALNCAVRGHPKPKVVWMKNKMEIREDPKFLITN--YQGVLT--LNIRRPSPFDAGTYTCRAVNELG 1127
[Close]
BLAST of low quality protein: twitchin-like vs. SwissProt
Match: gi|81910387|sp|Q5XKE0.1|MYPC2_MOUSE (RecName: Full=Myosin-binding protein C, fast-type; Short=Fast MyBP-C; AltName: Full=C-protein, skeletal muscle fast isoform)

HSP 1 Score: 62.003 bits (149), Expect = 7.171e-9
Identity = 60/196 (30.61%), Postives = 82/196 (41.84%), Query Frame = 0 
Query:  243 LGRWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAG--DSPPSS----------------ETKPHLCRH--KNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNG 417
            L  W+ P DDG   IT Y +QK DK    WF+ +   ++  + T+ DL         G  Y FR+ + N  G  DSP  S                E K H  R   K L P +D+            +A  +  V+G P P+  W KN  ++    +F+I    YQG  T  L + R    DAGTY+  A N  G
Sbjct:  945 LVEWQPPKDDGNSEITGYFVQKADKKTMEWFN-VYEHNRHTSCTVSDLIV-------GNEYYFRIFSENICGLSDSPGVSKNTARILKTGITLKPLEYKEHDFRTAPKFLTPLMDRVVVAG------YTAALNCAVRGHPKPKVVWMKNKMEIHEDPKFLITN--YQGILT--LNIRRPSPFDAGTYSCRAFNELG 1122
[Close]
BLAST of low quality protein: twitchin-like vs. SwissProt
Match: gi|1076761195|sp|D3YXG0.1|HMCN1_MOUSE (RecName: Full=Hemicentin-1; AltName: Full=Fibulin-6; Short=FIBL-6; Flags: Precursor)

HSP 1 Score: 62.003 bits (149), Expect = 8.123e-9
Identity = 42/131 (32.06%), Postives = 66/131 (50.38%), Query Frame = 0 
Query:  303 QFRVVAANKAG----DSPPSSETKPHLCRHKNLRPCIDKGAAG-SKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            ++  VA+N AG    D   + +  P   +   +   +D G +G +K V ++     H +    PPP  +WYK+G  L +SD  +I      GG   +LQ+ R+++ DAG YT VA N  G D +  D+ VL
Sbjct: 2741 RYTCVASNLAGEDELDFDVNIQVPPSFQKLWEIGNMLDTGRSGEAKDVIINNPLSLHCETNAAPPPTLTWYKDGRPLTSSDRVLI----LPGG--RVLQIPRAKVEDAGRYTCVAVNEAGEDSLRYDVHVL 2865
[Close]
BLAST of low quality protein: twitchin-like vs. SwissProt
Match: gi|85542049|sp|Q96RW7.2|HMCN1_HUMAN (RecName: Full=Hemicentin-1; AltName: Full=Fibulin-6; Short=FIBL-6; Flags: Precursor)

HSP 1 Score: 60.4622 bits (145), Expect = 2.227e-8
Identity = 41/131 (31.30%), Postives = 67/131 (51.15%), Query Frame = 0 
Query:  303 QFRVVAANKAG----DSPPSSETKPHLCRHKNLRPCIDKGAAG-SKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            ++  VA+N AG    D   + +  P   +   +   +D G  G +K V ++     + +    PPP  +WYK+G+ L +SD+ +I      GG   +LQ+ R+++ DAG YT VA N  G D +  D+ VL
Sbjct: 2742 RYTCVASNIAGEDELDFDVNIQVPPSFQKLWEIGNMLDTGRNGEAKDVIINNPISLYCETNAAPPPTLTWYKDGHPLTSSDKVLI----LPGGR--VLQIPRAKVEDAGRYTCVAVNEAGEDSLQYDVRVL 2866
[Close]
BLAST of low quality protein: twitchin-like vs. SwissProt
Match: gi|6166595|sp|O70468.1|MYPC3_MOUSE (RecName: Full=Myosin-binding protein C, cardiac-type; Short=Cardiac MyBP-C; AltName: Full=C-protein, cardiac muscle isoform)

HSP 1 Score: 55.4546 bits (132), Expect = 8.616e-7
Identity = 57/198 (28.79%), Postives = 78/198 (39.39%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKA-CTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETK-------PHLCRHKNLRPCIDKGAAGS-------KSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            W+ P DDG   I  Y +QK DK    WF  L    +  C          V  L  G  Y FRV + N  G S  ++ TK       P +         +D   A S       +S+    +AI    V+G P P+ SW+KNG  L     F +     QG  T  L++ +   +D G Y   A N  G  + +  L V
Sbjct: 1082 WKPPQDDGNTEIWGYTVQKADKKTMEWFTVLEHYRRTHCV---------VSELIIGNGYYFRVFSHNMVGSSDKAAATKEPVFIPRPGITYEPPKYKALDFSEAPSFTQPLANRSIIAGYNAILCCAVRGSPKPKISWFKNGLDLGEDARFRMFCK--QGVLT--LEIRKPCPYDGGVYVCRATNLQGEAQCECRLEV 1266
[Close]
BLAST of low quality protein: twitchin-like vs. nr
Match: gi|1325263294|ref|XP_023333663.1| (twitchin-like isoform X4 [Eurytemora affinis])

HSP 1 Score: 296.975 bits (759), Expect = 1.623e-85
Identity = 133/209 (63.64%), Postives = 165/209 (78.95%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMDDGDCNMFKHGMRGCTCSAGF 452
            RW+KP  DGGRPI+HYIIQKKDKFGGWFDAL+TDD+ C ATID+LEA+VPGLSEGKWYQFRV+A NKAG+S PS ETKPHLCRHKNL P IDKG AGSK+VR++R+A W I+ KGEPPP F+W +    +L + + + + TDEYQGGAT  L +  S + D GTYTL AENRNG +KIDLDL+VL  + + DC+M++ G + C+C   +
Sbjct: 1112 RWDKPKHDGGRPISHYIIQKKDKFGGWFDALITDDQNCVATIDELEARVPGLSEGKWYQFRVIAVNKAGESDPSPETKPHLCRHKNLSPSIDKGQAGSKTVRINRTAFWQIRCKGEPPPVFTWSHPVHGELKSCENYTVHTDEYQGGATTTLVIHHSTLQDGGTYTLQAENRNGKEKIDLDLIVLDNLPECDCDMYRRGDKQCSCQHSY 1320

HSP 2 Score: 84.7297 bits (208), Expect = 1.693e-13
Identity = 54/171 (31.58%), Postives = 86/171 (50.29%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDK-FGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEF----VIATDEYQGGATAMLQVFRS-QMHDAGTYTL 410
            W+ P  DGG PITHYII+ K+K  G W +A V   K   A  + ++ K  GL EG+ YQFR+ A NK G S P   +   + +H+ + P +        +++  R   + +   GEP P   W +NG + +NSDE     + + +        +L + ++ +  D G YT+
Sbjct:  804 WDPPASDGGAPITHYIIEMKEKNMGQWVEAKVLTLKEVEALGNKIKGKCDGLVEGQEYQFRIKAVNKGGPSKPGPPSDSMIAKHRFIPPHLLGDGIYDITLKKGRPIRYDLWFGGEPAPSVEWIRNG-RTLNSDEHTSIELYSKNTIYTEKNTVLSIPKADRARDTGEYTI 973
[Close]
BLAST of low quality protein: twitchin-like vs. nr
Match: gi|1325263307|ref|XP_023333672.1| (twitchin-like isoform X5 [Eurytemora affinis])

HSP 1 Score: 296.59 bits (758), Expect = 1.800e-85
Identity = 133/209 (63.64%), Postives = 165/209 (78.95%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMDDGDCNMFKHGMRGCTCSAGF 452
            RW+KP  DGGRPI+HYIIQKKDKFGGWFDAL+TDD+ C ATID+LEA+VPGLSEGKWYQFRV+A NKAG+S PS ETKPHLCRHKNL P IDKG AGSK+VR++R+A W I+ KGEPPP F+W +    +L + + + + TDEYQGGAT  L +  S + D GTYTL AENRNG +KIDLDL+VL  + + DC+M++ G + C+C   +
Sbjct: 1093 RWDKPKHDGGRPISHYIIQKKDKFGGWFDALITDDQNCVATIDELEARVPGLSEGKWYQFRVIAVNKAGESDPSPETKPHLCRHKNLSPSIDKGQAGSKTVRINRTAFWQIRCKGEPPPVFTWSHPVHGELKSCENYTVHTDEYQGGATTTLVIHHSTLQDGGTYTLQAENRNGKEKIDLDLIVLDNLPECDCDMYRRGDKQCSCQHSY 1301

HSP 2 Score: 84.7297 bits (208), Expect = 1.687e-13
Identity = 54/171 (31.58%), Postives = 86/171 (50.29%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDK-FGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEF----VIATDEYQGGATAMLQVFRS-QMHDAGTYTL 410
            W+ P  DGG PITHYII+ K+K  G W +A V   K   A  + ++ K  GL EG+ YQFR+ A NK G S P   +   + +H+ + P +        +++  R   + +   GEP P   W +NG + +NSDE     + + +        +L + ++ +  D G YT+
Sbjct:  785 WDPPASDGGAPITHYIIEMKEKNMGQWVEAKVLTLKEVEALGNKIKGKCDGLVEGQEYQFRIKAVNKGGPSKPGPPSDSMIAKHRFIPPHLLGDGIYDITLKKGRPIRYDLWFGGEPAPSVEWIRNG-RTLNSDEHTSIELYSKNTIYTEKNTVLSIPKADRARDTGEYTI 954
[Close]
BLAST of low quality protein: twitchin-like vs. nr
Match: gi|1325307310|ref|XP_023335272.1| (twitchin-like [Eurytemora affinis])

HSP 1 Score: 271.552 bits (693), Expect = 1.257e-76
Identity = 132/228 (57.89%), Postives = 165/228 (72.37%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDE---FVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMDDGDCNMFKHGMRGCTCSAGFRHNELSAQQILTVDFGD 469
            RW KP +DGGRPITHY+IQKKD+FGGW+DAL TD+ +C ATI +LEA++PGLS GK Y FRV+A  KAGDS PS ETKPHLCR+KNL P IDKG+ GSK+V+V+R   + IKVKGEP P+FSW K+G K IN  E     +  +     A A LQ+ R+QM DAGTY+L+AENRNG D +DLDL+VL ++   DC MF +G+  CTC+  FR  E+S Q  L +   D
Sbjct: 1113 RWAKPANDGGRPITHYVIQKKDEFGGWYDALKTDNDSCFATIAELEARIPGLSLGKRYTFRVIAITKAGDSEPSRETKPHLCRYKNLSPAIDKGSGGSKTVKVNRLTCFQIKVKGEPIPEFSWKKDGQK-INPAEGLSITVTHNPEDQSAIATLQILRTQMTDAGTYSLLAENRNGFDSVDLDLIVLDEIHTNDCEMFLNGVLACTCNNRFRA-EISDQDSLKLGLQD 1338
[Close]
BLAST of low quality protein: twitchin-like vs. nr
Match: gi|1068388476|ref|XP_018043887.1| (PREDICTED: twitchin isoform X21 [Atta colombica])

HSP 1 Score: 151.369 bits (381), Expect = 2.849e-35
Identity = 82/190 (43.16%), Postives = 107/190 (56.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMD 432
            +WEKP +DGGRPITHY ++ KDKF   W + + T D          EAKV GL E   YQFRV A NKAG S PS  T  HLC+H+NLRP ID+    S  ++  R+  W + V GEPPP+  W +++   L  ++   I   EY    T    +  +   D G YTLVAEN NG D+  ++L VL + D
Sbjct: 1345 KWEKPQNDGGRPITHYTVEIKDKFAVDWVEVMKTTDSTP-------EAKVEGLKEKMVYQFRVRAHNKAGSSEPSEPTDNHLCKHRNLRPRIDRTNFKSVIIKAGRTHKWSVDVSGEPPPEIRWIWRDNIPLTTTERIKIENVEYHTDFT----IVNAMRKDTGKYTLVAENANGKDEETVELTVLGKPD 1523

HSP 2 Score: 124.79 bits (312), Expect = 2.036e-26
Identity = 67/186 (36.02%), Postives = 102/186 (54.84%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            +W  P  DGG PI  YII+KKD++   W       +KA     + LE KVP L E   YQFRV+A NKAG SPPS  +K  +C+HK L+P ID+       VR  ++  + + V+GEPPP+  WY  GN++ + +   I   +Y       L +  S   + G + + A N +G D+ ++++ +L+
Sbjct: 1645 KWAPPEFDGGAPIEKYIIEKKDRYKPDW-------EKAIEVPGNQLEGKVPDLKERGEYQFRVIAVNKAGPSPPSDASKMQICKHKALKPRIDRTNLKPIIVRAGKTVKYDVDVRGEPPPEIKWYHVGNEVKSGENIEIINIDYN----TKLTITDSLRKNTGVWKIKAVNPHGEDEAEVEVTILS 1819

HSP 3 Score: 116.701 bits (291), Expect = 9.681e-24
Identity = 67/187 (35.83%), Postives = 100/187 (53.48%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFD-ALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            W  P DDGG PI  YI+Q +DK G  W D A V+ D+      D       G+ EG  Y+FR+VA NKAG   PS  +K  + + + L P ID+     K +R+ +       +KGEPPP  +W      L + D   I  ++Y      M  + + Q  D GTYT++A+N +G+D++D++L VL++
Sbjct: 2533 WTPPKDDGGAPIEKYIVQMRDKEGRQWVDVAKVSGDRTAAKVTD-------GIQEGHEYEFRIVAVNKAGPGEPSDTSKSVVAKPRFLAPHIDRKNLHKKIMRIGQMLRLEADIKGEPPPIVTWKLKDKVLKSMDRLKIENEDYH----TMFILNKLQRSDTGTYTVIAKNDSGTDQVDVELQVLSK 2708

HSP 4 Score: 116.316 bits (290), Expect = 1.238e-23
Identity = 67/185 (36.22%), Postives = 98/185 (52.97%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +WE P D GG PIT YII+KK+K    W +ALVTD           + +V GL +G  YQFR+ A NKAG S PS  TKPH+ + + L+P I++    +  VR  +     + V+GEPPP  +W   G  L       I  ++Y       +Q+  +     G YT+ A N +G D+ D+++ +L
Sbjct: 1940 KWEPPKDTGGAPITGYIIEKKEKPSSQWEEALVTDSP-------QPKGRVTGLKKGSTYQFRIRAVNKAGPSEPSDPTKPHVAKARFLKPHINRDKLQTIKVRAGQLVKLEVDVEGEPPPTVTWSFAGAPLSTGANIKIDNEDY----LTKIQLINTSRKLTGKYTIKAVNDSGQDEADVEINIL 2113

HSP 5 Score: 115.931 bits (289), Expect = 1.343e-23
Identity = 65/187 (34.76%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWF-DALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            +WE P+ DGG PIT YII+ KDKFG  F  A       CT T       VP L EG  YQFR+ A NKAG   PS  T PH  + + L+P ID+      +V+V  +    + + GEPPP+ +W     +L++ D   I   +Y         + +++    G Y + A+N  G D  ++++ VL +
Sbjct: 2237 KWEPPIRDGGAPITGYIIEMKDKFGTNFVKAAEVQGPVCTGT-------VPRLEEGNQYQFRIRAVNKAGPGEPSEATNPHTAKARWLKPYIDRTNLQPITVKVGLTVSLDVNIIGEPPPKVTWTFKDKELVSDDTIRIDNVDYN----TKFFILKTKRAHTGKYVINAKNEVGEDTAEVEITVLGK 2412

HSP 6 Score: 115.931 bits (289), Expect = 1.626e-23
Identity = 66/184 (35.87%), Postives = 99/184 (53.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+ P  DGG PI  YII+KKD++G W        KA     +  EAKVP L EG+ YQFRV A NK G S PS  ++  + + +   P ID+      +++        +KV GEPPP  +W+ N  K  + +   I T++Y+   T  +    ++ H  GT TL AEN +G D+  ++++VL
Sbjct: 2828 KWKAPKKDGGAPIEKYIIEKKDQYGKW-------QKAAEVPGNKTEAKVPDLVEGQKYQFRVKAVNKGGQSKPSEPSETLIAKDRYAAPKIDRTNLKDITIKAGSVIRLDVKVTGEPPPSKTWFLNKAKQESGNVLTIETEDYK---TKFVVSSATRAH-CGTLTLKAENSSGKDEASIEILVL 3000

HSP 7 Score: 114.39 bits (285), Expect = 4.797e-23
Identity = 66/184 (35.87%), Postives = 96/184 (52.17%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT Y+I+KKDK+G W       +K      D+  A VP L EG+ Y+FRV A NKAG   PS  T   + + +NL P ID+       ++  ++  +  KV GEPPP   W  NG ++  S+   +   +Y       L V  +   ++G YT+ AEN NG D+  + + VL
Sbjct: 3711 KWTPPENDGGSPITGYVIEKKDKYGEW-------EKTLEVPADETFATVPDLIEGQPYEFRVRAVNKAGPGEPSDATPTIIAKPRNLAPRIDRTNLIEVKIKAGQNFNFDCKVTGEPPPTTKWMLNGKEVRPSERVKVKHVDYN----TSLNVRMATRAESGKYTITAENINGRDEAFVKVTVL 3883

HSP 8 Score: 114.39 bits (285), Expect = 5.497e-23
Identity = 69/184 (37.50%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W +P +DGG PIT YII+K+DK+        T +K      D    +V  L EG  Y+FRV A NKAG    S  +K H+ R KNL P ID+       VR      + + V GEPPP   W    N ++ SD   I  ++Y       ++V  ++  D+G YTLVA+N NG D   L + VL
Sbjct: 4007 QWSRPEEDGGSPITGYIIEKRDKYSP------TWEKCAEIDGDTNRGRVDDLVEGTPYEFRVRAVNKAGPGEASEASKSHIARPKNLPPKIDRKYMLDVKVRAGSFFDFDVPVTGEPPPSKEWSLKDNVVMASDRIKIINEDYN----TKVRVMEAKRSDSGIYTLVAKNVNGKDSATLTVNVL 4180

HSP 9 Score: 103.219 bits (256), Expect = 2.409e-19
Identity = 64/184 (34.78%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP +DGG PIT YII+ K+KFG  W      +     ATID       GL EG  Y+FR+ A NKAG   PS  TKP + + + ++P I      +  V+  +   + IK  GEP P+  W+    ++I      +  D+Y+     +L V ++   D+G Y LV  N +G+ +   D+VVL
Sbjct: 1047 WTKPENDGGAPITGYIIEYKEKFGKEWVTGKEIEGDVTQATID-------GLKEGTQYEFRIRAVNKAGPGEPSDVTKPIIAKCRFVKPYIVGDGLTNLVVKKGQVIKYDIKYAGEPEPEVHWFLGQKEIIQDSAERVTIDKYE--RNTVLTVRKTTRPDSGKYKLVLTNSSGTCESVADVVVL 1221

HSP 10 Score: 100.908 bits (250), Expect = 1.088e-18
Identity = 63/189 (33.33%), Postives = 97/189 (51.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVF--RSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            RW  PL DGG PIT Y+I+K++K    W        KAC  T  D + +V  L EG  Y+FRV A N AG   PS  +KP   + + L P ID+      +VR + +  + +K+ GEPPP  +W  N   +  +    I    Y        + F  + +  D+GTY + A N++GSD  ++++ V+++
Sbjct: 3122 RWTPPLKDGGSPITGYVIEKREKGAPKWI-------KACE-TGPDCKGRVDNLDEGVEYEFRVKAINAAGLGEPSDASKPITAKSRRLPPKIDRRNLRDITVRENEAFHFDVKIIGEPPPDVTWVINNKSIQQTTHRRIQNVPYNS------KFFNDKPERKDSGTYKITAVNQHGSDTAEVEVTVVSK 3296

HSP 11 Score: 96.6709 bits (239), Expect = 2.850e-17
Identity = 55/183 (30.05%), Postives = 86/183 (46.99%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG PIT YII+K+ +FG W       +KA     +    +VP L+EG+ Y+FRV+A NK G   PS  + P + + + + P  D        VR  +   ++I +   P P+ +W ++G +++         + Y        ++  S   D G YTL  EN +GS      + VL
Sbjct: 4303 WTPPKKDGGSPITSYIIEKRPRFGQW-------EKAAEIPGNQTSGRVPDLTEGQEYEFRVIAVNKGGPGEPSEASAPIVAKPRFIAPSFDTHLLHDLVVRAGQKISYNIPIVASPKPKATWNRDGVQIVADARH----EMYTTNTETSFEIPFSVRSDTGRYTLTLENEHGSFSASARVTVL 4474

HSP 12 Score: 94.3597 bits (233), Expect = 1.578e-16
Identity = 64/183 (34.97%), Postives = 90/183 (49.18%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            W++P+ DGG PIT YII+KKDK+   W  AL T            +A V GL EG  YQFRV+A NKAG S P   +K    + + L P ID+      ++       +   V GEPPP   W + G   ++SD  V   +       ++  V R    D+G YT+ A N +G D + + + V
Sbjct: 3417 WKEPVSDGGSPITGYIIEKKDKYSTMWEKALETGTPVT-------KALVHGLIEGNDYQFRVIAVNKAGQSEPGEASKTFTAKPRFLAPKIDRRNLRDVTLSAGSMLKFDANVIGEPPPHIEW-RYGAIPLHSDRKVQIDNIDYNTKFSIRPVSRD---DSGDYTVTASNSSGKDSVTVQVTV 3588

HSP 13 Score: 92.4337 bits (228), Expect = 5.832e-16
Identity = 60/187 (32.09%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVK--GEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT YI+QKK+K    W +A+       + T       VP L+EG+ Y+FRV+A N AG S PS  +     + + L P I       + VR+    I+H+ +   GEP P+ SW    N+L ++D   I +  Y      ++ +  ++  D+G Y L+ +N +G D+    + VL
Sbjct: 4596 QWPVPKNDGGSPITGYIVQKKEKGSPYWVNAVHVPAGQNSTT-------VPDLTEGQDYEFRVIAVNAAGQSEPSEPSDLVTAKPRFLAPKIKTPL---QDVRIKAGLIFHVDIDFIGEPTPEASWSVGSNELTSNDRTTITSIGYH----TIVHIVNAKRSDSGLYHLLLKNSSGIDEGSFQVTVL 4768

HSP 14 Score: 75.485 bits (184), Expect = 1.358e-10
Identity = 58/184 (31.52%), Postives = 76/184 (41.30%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG  I  YIIQKK K    W D          A I      VP L EG+ Y FRV A N  G S PS  + P +   +  +P +D G     +VR       H+   G P P  +W+ N    +  DE      +      A L V  ++  D G Y L   N +G D   +++ VL
Sbjct: 5292 WRSPRSDGGSKIRGYIIQKKAKGDDDWSDV-------NGAPIPSNVYTVPNLKEGEEYLFRVFAVNDVGSSDPSRPSNPIVIEEQPNKPVMDLGGVRDITVRAGEDFSIHVPYIGFPKPTATWFAND---VIKDETDPRVHQQLADDFASLVVKNAKRSDGGQYRLQLRNSSGFDTATVNVKVL 5465

HSP 15 Score: 67.3958 bits (163), Expect = 5.742e-8
Identity = 33/88 (37.50%), Postives = 47/88 (53.41%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W  P++ GG+PIT Y ++K+++ G W           T T+ DL        EG  Y+FRV A N+AG   PS  T+P +  H+ LRP
Sbjct: 5192 WNPPINTGGKPITGYYVEKRERGGEWLKVNNYPTPNTTFTVQDLH-------EGSRYEFRVSAVNEAGPGKPSKPTEPIIAGHQRLRP 5272

HSP 16 Score: 65.0846 bits (157), Expect = 2.677e-7
Identity = 54/234 (23.08%), Postives = 102/234 (43.59%), Query Frame = 0 
Query:  211 RLELNGLLGQLGEELLAEEHVLSRLQLLLPMHLGR--------------WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKV--KGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            R+    L G+  E L  +  V+++ Q ++P   G+              W  P+ +GG  I  Y ++++D+  G +     +        DD       ++EG  Y++RV A N AG    S  +     +    +P +   A   + ++V      ++ +   G P P+  W K+G  L+   E +  + E +   T +L + +S   D G YT+ A N +G D  D++++V+
Sbjct: 4847 RVSAENLQGR-SEPLTTDRSVVAKNQFVVPGQPGKPECVDADKDHIKIKWTPPISNGGSKIIGYDVERRDRATGRWIKQNKEPVRYPEYYDD------HVTEGHQYEYRVSAINAAGAGKASETSSVFTAKPMKEKPKLYLDALIGRKIKVRAGEPINVNIPLSGAPTPKIEWTKDGRSLM---ETLRLSTETKSDRTQLL-IEKSVRDDGGMYTVTATNEHGKDSADIEVIVV 5069

HSP 17 Score: 63.5438 bits (153), Expect = 7.355e-7
Identity = 52/187 (27.81%), Postives = 80/187 (42.78%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDD---KACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKG-AAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG PI  Y+I+K+         L+ +D   KA  A + D   KV GL E   Y+FRV A N AG  P SS +           P I    +    +V         +     P P+ +W  NG +++  +     T +    A+  +   +++  D GTYT+   N  G+D     ++V+
Sbjct: 5588 WTKPRHDGGSPILGYVIEKR---------LLNEDKWVKATPALVHDTTYKVTGLIENHDYEFRVAAENAAGRGPWSSNSDVIRASAAPFPPKITSDLSIRDMTVIAGEPFTITVPYTANPKPRPNWSINGEEVLTGERIKFETTDV---ASQFINK-KAKRSDTGTYTIYLTNTVGTDSASCKVLVV 5761
[Close]
BLAST of low quality protein: twitchin-like vs. nr
Match: gi|1068388472|ref|XP_018043886.1| (PREDICTED: twitchin isoform X20 [Atta colombica])

HSP 1 Score: 151.369 bits (381), Expect = 2.849e-35
Identity = 82/190 (43.16%), Postives = 107/190 (56.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMD 432
            +WEKP +DGGRPITHY ++ KDKF   W + + T D          EAKV GL E   YQFRV A NKAG S PS  T  HLC+H+NLRP ID+    S  ++  R+  W + V GEPPP+  W +++   L  ++   I   EY    T    +  +   D G YTLVAEN NG D+  ++L VL + D
Sbjct: 1361 KWEKPQNDGGRPITHYTVEIKDKFAVDWVEVMKTTDSTP-------EAKVEGLKEKMVYQFRVRAHNKAGSSEPSEPTDNHLCKHRNLRPRIDRTNFKSVIIKAGRTHKWSVDVSGEPPPEIRWIWRDNIPLTTTERIKIENVEYHTDFT----IVNAMRKDTGKYTLVAENANGKDEETVELTVLGKPD 1539

HSP 2 Score: 124.79 bits (312), Expect = 2.036e-26
Identity = 67/186 (36.02%), Postives = 102/186 (54.84%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            +W  P  DGG PI  YII+KKD++   W       +KA     + LE KVP L E   YQFRV+A NKAG SPPS  +K  +C+HK L+P ID+       VR  ++  + + V+GEPPP+  WY  GN++ + +   I   +Y       L +  S   + G + + A N +G D+ ++++ +L+
Sbjct: 1661 KWAPPEFDGGAPIEKYIIEKKDRYKPDW-------EKAIEVPGNQLEGKVPDLKERGEYQFRVIAVNKAGPSPPSDASKMQICKHKALKPRIDRTNLKPIIVRAGKTVKYDVDVRGEPPPEIKWYHVGNEVKSGENIEIINIDYN----TKLTITDSLRKNTGVWKIKAVNPHGEDEAEVEVTILS 1835

HSP 3 Score: 116.701 bits (291), Expect = 9.682e-24
Identity = 67/187 (35.83%), Postives = 100/187 (53.48%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFD-ALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            W  P DDGG PI  YI+Q +DK G  W D A V+ D+      D       G+ EG  Y+FR+VA NKAG   PS  +K  + + + L P ID+     K +R+ +       +KGEPPP  +W      L + D   I  ++Y      M  + + Q  D GTYT++A+N +G+D++D++L VL++
Sbjct: 2549 WTPPKDDGGAPIEKYIVQMRDKEGRQWVDVAKVSGDRTAAKVTD-------GIQEGHEYEFRIVAVNKAGPGEPSDTSKSVVAKPRFLAPHIDRKNLHKKIMRIGQMLRLEADIKGEPPPIVTWKLKDKVLKSMDRLKIENEDYH----TMFILNKLQRSDTGTYTVIAKNDSGTDQVDVELQVLSK 2724

HSP 4 Score: 116.316 bits (290), Expect = 1.238e-23
Identity = 67/185 (36.22%), Postives = 98/185 (52.97%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +WE P D GG PIT YII+KK+K    W +ALVTD           + +V GL +G  YQFR+ A NKAG S PS  TKPH+ + + L+P I++    +  VR  +     + V+GEPPP  +W   G  L       I  ++Y       +Q+  +     G YT+ A N +G D+ D+++ +L
Sbjct: 1956 KWEPPKDTGGAPITGYIIEKKEKPSSQWEEALVTDSP-------QPKGRVTGLKKGSTYQFRIRAVNKAGPSEPSDPTKPHVAKARFLKPHINRDKLQTIKVRAGQLVKLEVDVEGEPPPTVTWSFAGAPLSTGANIKIDNEDY----LTKIQLINTSRKLTGKYTIKAVNDSGQDEADVEINIL 2129

HSP 5 Score: 115.931 bits (289), Expect = 1.343e-23
Identity = 65/187 (34.76%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWF-DALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            +WE P+ DGG PIT YII+ KDKFG  F  A       CT T       VP L EG  YQFR+ A NKAG   PS  T PH  + + L+P ID+      +V+V  +    + + GEPPP+ +W     +L++ D   I   +Y         + +++    G Y + A+N  G D  ++++ VL +
Sbjct: 2253 KWEPPIRDGGAPITGYIIEMKDKFGTNFVKAAEVQGPVCTGT-------VPRLEEGNQYQFRIRAVNKAGPGEPSEATNPHTAKARWLKPYIDRTNLQPITVKVGLTVSLDVNIIGEPPPKVTWTFKDKELVSDDTIRIDNVDYN----TKFFILKTKRAHTGKYVINAKNEVGEDTAEVEITVLGK 2428

HSP 6 Score: 115.931 bits (289), Expect = 1.626e-23
Identity = 66/184 (35.87%), Postives = 99/184 (53.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+ P  DGG PI  YII+KKD++G W        KA     +  EAKVP L EG+ YQFRV A NK G S PS  ++  + + +   P ID+      +++        +KV GEPPP  +W+ N  K  + +   I T++Y+   T  +    ++ H  GT TL AEN +G D+  ++++VL
Sbjct: 2844 KWKAPKKDGGAPIEKYIIEKKDQYGKW-------QKAAEVPGNKTEAKVPDLVEGQKYQFRVKAVNKGGQSKPSEPSETLIAKDRYAAPKIDRTNLKDITIKAGSVIRLDVKVTGEPPPSKTWFLNKAKQESGNVLTIETEDYK---TKFVVSSATRAH-CGTLTLKAENSSGKDEASIEILVL 3016

HSP 7 Score: 114.39 bits (285), Expect = 4.797e-23
Identity = 66/184 (35.87%), Postives = 96/184 (52.17%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT Y+I+KKDK+G W       +K      D+  A VP L EG+ Y+FRV A NKAG   PS  T   + + +NL P ID+       ++  ++  +  KV GEPPP   W  NG ++  S+   +   +Y       L V  +   ++G YT+ AEN NG D+  + + VL
Sbjct: 3727 KWTPPENDGGSPITGYVIEKKDKYGEW-------EKTLEVPADETFATVPDLIEGQPYEFRVRAVNKAGPGEPSDATPTIIAKPRNLAPRIDRTNLIEVKIKAGQNFNFDCKVTGEPPPTTKWMLNGKEVRPSERVKVKHVDYN----TSLNVRMATRAESGKYTITAENINGRDEAFVKVTVL 3899

HSP 8 Score: 114.39 bits (285), Expect = 5.498e-23
Identity = 69/184 (37.50%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W +P +DGG PIT YII+K+DK+        T +K      D    +V  L EG  Y+FRV A NKAG    S  +K H+ R KNL P ID+       VR      + + V GEPPP   W    N ++ SD   I  ++Y       ++V  ++  D+G YTLVA+N NG D   L + VL
Sbjct: 4023 QWSRPEEDGGSPITGYIIEKRDKYSP------TWEKCAEIDGDTNRGRVDDLVEGTPYEFRVRAVNKAGPGEASEASKSHIARPKNLPPKIDRKYMLDVKVRAGSFFDFDVPVTGEPPPSKEWSLKDNVVMASDRIKIINEDYN----TKVRVMEAKRSDSGIYTLVAKNVNGKDSATLTVNVL 4196

HSP 9 Score: 103.219 bits (256), Expect = 2.409e-19
Identity = 64/184 (34.78%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP +DGG PIT YII+ K+KFG  W      +     ATID       GL EG  Y+FR+ A NKAG   PS  TKP + + + ++P I      +  V+  +   + IK  GEP P+  W+    ++I      +  D+Y+     +L V ++   D+G Y LV  N +G+ +   D+VVL
Sbjct: 1063 WTKPENDGGAPITGYIIEYKEKFGKEWVTGKEIEGDVTQATID-------GLKEGTQYEFRIRAVNKAGPGEPSDVTKPIIAKCRFVKPYIVGDGLTNLVVKKGQVIKYDIKYAGEPEPEVHWFLGQKEIIQDSAERVTIDKYE--RNTVLTVRKTTRPDSGKYKLVLTNSSGTCESVADVVVL 1237

HSP 10 Score: 100.908 bits (250), Expect = 1.088e-18
Identity = 63/189 (33.33%), Postives = 97/189 (51.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVF--RSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            RW  PL DGG PIT Y+I+K++K    W        KAC  T  D + +V  L EG  Y+FRV A N AG   PS  +KP   + + L P ID+      +VR + +  + +K+ GEPPP  +W  N   +  +    I    Y        + F  + +  D+GTY + A N++GSD  ++++ V+++
Sbjct: 3138 RWTPPLKDGGSPITGYVIEKREKGAPKWI-------KACE-TGPDCKGRVDNLDEGVEYEFRVKAINAAGLGEPSDASKPITAKSRRLPPKIDRRNLRDITVRENEAFHFDVKIIGEPPPDVTWVINNKSIQQTTHRRIQNVPYNS------KFFNDKPERKDSGTYKITAVNQHGSDTAEVEVTVVSK 3312

HSP 11 Score: 96.6709 bits (239), Expect = 2.850e-17
Identity = 55/183 (30.05%), Postives = 86/183 (46.99%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG PIT YII+K+ +FG W       +KA     +    +VP L+EG+ Y+FRV+A NK G   PS  + P + + + + P  D        VR  +   ++I +   P P+ +W ++G +++         + Y        ++  S   D G YTL  EN +GS      + VL
Sbjct: 4319 WTPPKKDGGSPITSYIIEKRPRFGQW-------EKAAEIPGNQTSGRVPDLTEGQEYEFRVIAVNKGGPGEPSEASAPIVAKPRFIAPSFDTHLLHDLVVRAGQKISYNIPIVASPKPKATWNRDGVQIVADARH----EMYTTNTETSFEIPFSVRSDTGRYTLTLENEHGSFSASARVTVL 4490

HSP 12 Score: 94.3597 bits (233), Expect = 1.578e-16
Identity = 64/183 (34.97%), Postives = 90/183 (49.18%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            W++P+ DGG PIT YII+KKDK+   W  AL T            +A V GL EG  YQFRV+A NKAG S P   +K    + + L P ID+      ++       +   V GEPPP   W + G   ++SD  V   +       ++  V R    D+G YT+ A N +G D + + + V
Sbjct: 3433 WKEPVSDGGSPITGYIIEKKDKYSTMWEKALETGTPVT-------KALVHGLIEGNDYQFRVIAVNKAGQSEPGEASKTFTAKPRFLAPKIDRRNLRDVTLSAGSMLKFDANVIGEPPPHIEW-RYGAIPLHSDRKVQIDNIDYNTKFSIRPVSRD---DSGDYTVTASNSSGKDSVTVQVTV 3604

HSP 13 Score: 92.4337 bits (228), Expect = 5.833e-16
Identity = 60/187 (32.09%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVK--GEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT YI+QKK+K    W +A+       + T       VP L+EG+ Y+FRV+A N AG S PS  +     + + L P I       + VR+    I+H+ +   GEP P+ SW    N+L ++D   I +  Y      ++ +  ++  D+G Y L+ +N +G D+    + VL
Sbjct: 4612 QWPVPKNDGGSPITGYIVQKKEKGSPYWVNAVHVPAGQNSTT-------VPDLTEGQDYEFRVIAVNAAGQSEPSEPSDLVTAKPRFLAPKIKTPL---QDVRIKAGLIFHVDIDFIGEPTPEASWSVGSNELTSNDRTTITSIGYH----TIVHIVNAKRSDSGLYHLLLKNSSGIDEGSFQVTVL 4784

HSP 14 Score: 75.485 bits (184), Expect = 1.370e-10
Identity = 58/184 (31.52%), Postives = 76/184 (41.30%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG  I  YIIQKK K    W D          A I      VP L EG+ Y FRV A N  G S PS  + P +   +  +P +D G     +VR       H+   G P P  +W+ N    +  DE      +      A L V  ++  D G Y L   N +G D   +++ VL
Sbjct: 5308 WRSPRSDGGSKIRGYIIQKKAKGDDDWSDV-------NGAPIPSNVYTVPNLKEGEEYLFRVFAVNDVGSSDPSRPSNPIVIEEQPNKPVMDLGGVRDITVRAGEDFSIHVPYIGFPKPTATWFAND---VIKDETDPRVHQQLADDFASLVVKNAKRSDGGQYRLQLRNSSGFDTATVNVKVL 5481

HSP 15 Score: 67.3958 bits (163), Expect = 5.743e-8
Identity = 33/88 (37.50%), Postives = 47/88 (53.41%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W  P++ GG+PIT Y ++K+++ G W           T T+ DL        EG  Y+FRV A N+AG   PS  T+P +  H+ LRP
Sbjct: 5208 WNPPINTGGKPITGYYVEKRERGGEWLKVNNYPTPNTTFTVQDLH-------EGSRYEFRVSAVNEAGPGKPSKPTEPIIAGHQRLRP 5288

HSP 16 Score: 65.0846 bits (157), Expect = 2.701e-7
Identity = 54/234 (23.08%), Postives = 102/234 (43.59%), Query Frame = 0 
Query:  211 RLELNGLLGQLGEELLAEEHVLSRLQLLLPMHLGR--------------WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKV--KGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            R+    L G+  E L  +  V+++ Q ++P   G+              W  P+ +GG  I  Y ++++D+  G +     +        DD       ++EG  Y++RV A N AG    S  +     +    +P +   A   + ++V      ++ +   G P P+  W K+G  L+   E +  + E +   T +L + +S   D G YT+ A N +G D  D++++V+
Sbjct: 4863 RVSAENLQGR-SEPLTTDRSVVAKNQFVVPGQPGKPECVDADKDHIKIKWTPPISNGGSKIIGYDVERRDRATGRWIKQNKEPVRYPEYYDD------HVTEGHQYEYRVSAINAAGAGKASETSSVFTAKPMKEKPKLYLDALIGRKIKVRAGEPINVNIPLSGAPTPKIEWTKDGRSLM---ETLRLSTETKSDRTQLL-IEKSVRDDGGMYTVTATNEHGKDSADIEVIVV 5085

HSP 17 Score: 63.5438 bits (153), Expect = 7.355e-7
Identity = 52/187 (27.81%), Postives = 80/187 (42.78%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDD---KACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKG-AAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG PI  Y+I+K+         L+ +D   KA  A + D   KV GL E   Y+FRV A N AG  P SS +           P I    +    +V         +     P P+ +W  NG +++  +     T +    A+  +   +++  D GTYT+   N  G+D     ++V+
Sbjct: 5604 WTKPRHDGGSPILGYVIEKR---------LLNEDKWVKATPALVHDTTYKVTGLIENHDYEFRVAAENAAGRGPWSSNSDVIRASAAPFPPKITSDLSIRDMTVIAGEPFTITVPYTANPKPRPNWSINGEEVLTGERIKFETTDV---ASQFINK-KAKRSDTGTYTIYLTNTVGTDSASCKVLVV 5777
[Close]
BLAST of low quality protein: twitchin-like vs. nr
Match: gi|1068388465|ref|XP_018043883.1| (PREDICTED: twitchin isoform X18 [Atta colombica])

HSP 1 Score: 151.369 bits (381), Expect = 2.853e-35
Identity = 82/190 (43.16%), Postives = 107/190 (56.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMD 432
            +WEKP +DGGRPITHY ++ KDKF   W + + T D          EAKV GL E   YQFRV A NKAG S PS  T  HLC+H+NLRP ID+    S  ++  R+  W + V GEPPP+  W +++   L  ++   I   EY    T    +  +   D G YTLVAEN NG D+  ++L VL + D
Sbjct: 1589 KWEKPQNDGGRPITHYTVEIKDKFAVDWVEVMKTTDSTP-------EAKVEGLKEKMVYQFRVRAHNKAGSSEPSEPTDNHLCKHRNLRPRIDRTNFKSVIIKAGRTHKWSVDVSGEPPPEIRWIWRDNIPLTTTERIKIENVEYHTDFT----IVNAMRKDTGKYTLVAENANGKDEETVELTVLGKPD 1767

HSP 2 Score: 124.79 bits (312), Expect = 2.039e-26
Identity = 67/186 (36.02%), Postives = 102/186 (54.84%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            +W  P  DGG PI  YII+KKD++   W       +KA     + LE KVP L E   YQFRV+A NKAG SPPS  +K  +C+HK L+P ID+       VR  ++  + + V+GEPPP+  WY  GN++ + +   I   +Y       L +  S   + G + + A N +G D+ ++++ +L+
Sbjct: 1889 KWAPPEFDGGAPIEKYIIEKKDRYKPDW-------EKAIEVPGNQLEGKVPDLKERGEYQFRVIAVNKAGPSPPSDASKMQICKHKALKPRIDRTNLKPIIVRAGKTVKYDVDVRGEPPPEIKWYHVGNEVKSGENIEIINIDYN----TKLTITDSLRKNTGVWKIKAVNPHGEDEAEVEVTILS 2063

HSP 3 Score: 116.701 bits (291), Expect = 9.695e-24
Identity = 67/187 (35.83%), Postives = 100/187 (53.48%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFD-ALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            W  P DDGG PI  YI+Q +DK G  W D A V+ D+      D       G+ EG  Y+FR+VA NKAG   PS  +K  + + + L P ID+     K +R+ +       +KGEPPP  +W      L + D   I  ++Y      M  + + Q  D GTYT++A+N +G+D++D++L VL++
Sbjct: 2777 WTPPKDDGGAPIEKYIVQMRDKEGRQWVDVAKVSGDRTAAKVTD-------GIQEGHEYEFRIVAVNKAGPGEPSDTSKSVVAKPRFLAPHIDRKNLHKKIMRIGQMLRLEADIKGEPPPIVTWKLKDKVLKSMDRLKIENEDYH----TMFILNKLQRSDTGTYTVIAKNDSGTDQVDVELQVLSK 2952

HSP 4 Score: 116.316 bits (290), Expect = 1.240e-23
Identity = 67/185 (36.22%), Postives = 98/185 (52.97%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +WE P D GG PIT YII+KK+K    W +ALVTD           + +V GL +G  YQFR+ A NKAG S PS  TKPH+ + + L+P I++    +  VR  +     + V+GEPPP  +W   G  L       I  ++Y       +Q+  +     G YT+ A N +G D+ D+++ +L
Sbjct: 2184 KWEPPKDTGGAPITGYIIEKKEKPSSQWEEALVTDSP-------QPKGRVTGLKKGSTYQFRIRAVNKAGPSEPSDPTKPHVAKARFLKPHINRDKLQTIKVRAGQLVKLEVDVEGEPPPTVTWSFAGAPLSTGANIKIDNEDY----LTKIQLINTSRKLTGKYTIKAVNDSGQDEADVEINIL 2357

HSP 5 Score: 115.931 bits (289), Expect = 1.345e-23
Identity = 65/187 (34.76%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWF-DALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            +WE P+ DGG PIT YII+ KDKFG  F  A       CT T       VP L EG  YQFR+ A NKAG   PS  T PH  + + L+P ID+      +V+V  +    + + GEPPP+ +W     +L++ D   I   +Y         + +++    G Y + A+N  G D  ++++ VL +
Sbjct: 2481 KWEPPIRDGGAPITGYIIEMKDKFGTNFVKAAEVQGPVCTGT-------VPRLEEGNQYQFRIRAVNKAGPGEPSEATNPHTAKARWLKPYIDRTNLQPITVKVGLTVSLDVNIIGEPPPKVTWTFKDKELVSDDTIRIDNVDYN----TKFFILKTKRAHTGKYVINAKNEVGEDTAEVEITVLGK 2656

HSP 6 Score: 115.931 bits (289), Expect = 1.628e-23
Identity = 66/184 (35.87%), Postives = 99/184 (53.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+ P  DGG PI  YII+KKD++G W        KA     +  EAKVP L EG+ YQFRV A NK G S PS  ++  + + +   P ID+      +++        +KV GEPPP  +W+ N  K  + +   I T++Y+   T  +    ++ H  GT TL AEN +G D+  ++++VL
Sbjct: 3072 KWKAPKKDGGAPIEKYIIEKKDQYGKW-------QKAAEVPGNKTEAKVPDLVEGQKYQFRVKAVNKGGQSKPSEPSETLIAKDRYAAPKIDRTNLKDITIKAGSVIRLDVKVTGEPPPSKTWFLNKAKQESGNVLTIETEDYK---TKFVVSSATRAH-CGTLTLKAENSSGKDEASIEILVL 3244

HSP 7 Score: 114.39 bits (285), Expect = 4.760e-23
Identity = 66/184 (35.87%), Postives = 96/184 (52.17%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT Y+I+KKDK+G W       +K      D+  A VP L EG+ Y+FRV A NKAG   PS  T   + + +NL P ID+       ++  ++  +  KV GEPPP   W  NG ++  S+   +   +Y       L V  +   ++G YT+ AEN NG D+  + + VL
Sbjct: 3955 KWTPPENDGGSPITGYVIEKKDKYGEW-------EKTLEVPADETFATVPDLIEGQPYEFRVRAVNKAGPGEPSDATPTIIAKPRNLAPRIDRTNLIEVKIKAGQNFNFDCKVTGEPPPTTKWMLNGKEVRPSERVKVKHVDYN----TSLNVRMATRAESGKYTITAENINGRDEAFVKVTVL 4127

HSP 8 Score: 114.39 bits (285), Expect = 5.505e-23
Identity = 69/184 (37.50%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W +P +DGG PIT YII+K+DK+        T +K      D    +V  L EG  Y+FRV A NKAG    S  +K H+ R KNL P ID+       VR      + + V GEPPP   W    N ++ SD   I  ++Y       ++V  ++  D+G YTLVA+N NG D   L + VL
Sbjct: 4251 QWSRPEEDGGSPITGYIIEKRDKYSP------TWEKCAEIDGDTNRGRVDDLVEGTPYEFRVRAVNKAGPGEASEASKSHIARPKNLPPKIDRKYMLDVKVRAGSFFDFDVPVTGEPPPSKEWSLKDNVVMASDRIKIINEDYN----TKVRVMEAKRSDSGIYTLVAKNVNGKDSATLTVNVL 4424

HSP 9 Score: 103.219 bits (256), Expect = 2.390e-19
Identity = 64/184 (34.78%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP +DGG PIT YII+ K+KFG  W      +     ATID       GL EG  Y+FR+ A NKAG   PS  TKP + + + ++P I      +  V+  +   + IK  GEP P+  W+    ++I      +  D+Y+     +L V ++   D+G Y LV  N +G+ +   D+VVL
Sbjct: 1291 WTKPENDGGAPITGYIIEYKEKFGKEWVTGKEIEGDVTQATID-------GLKEGTQYEFRIRAVNKAGPGEPSDVTKPIIAKCRFVKPYIVGDGLTNLVVKKGQVIKYDIKYAGEPEPEVHWFLGQKEIIQDSAERVTIDKYE--RNTVLTVRKTTRPDSGKYKLVLTNSSGTCESVADVVVL 1465

HSP 10 Score: 100.908 bits (250), Expect = 1.080e-18
Identity = 63/189 (33.33%), Postives = 97/189 (51.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVF--RSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            RW  PL DGG PIT Y+I+K++K    W        KAC  T  D + +V  L EG  Y+FRV A N AG   PS  +KP   + + L P ID+      +VR + +  + +K+ GEPPP  +W  N   +  +    I    Y        + F  + +  D+GTY + A N++GSD  ++++ V+++
Sbjct: 3366 RWTPPLKDGGSPITGYVIEKREKGAPKWI-------KACE-TGPDCKGRVDNLDEGVEYEFRVKAINAAGLGEPSDASKPITAKSRRLPPKIDRRNLRDITVRENEAFHFDVKIIGEPPPDVTWVINNKSIQQTTHRRIQNVPYNS------KFFNDKPERKDSGTYKITAVNQHGSDTAEVEVTVVSK 3540

HSP 11 Score: 96.6709 bits (239), Expect = 2.828e-17
Identity = 55/183 (30.05%), Postives = 86/183 (46.99%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG PIT YII+K+ +FG W       +KA     +    +VP L+EG+ Y+FRV+A NK G   PS  + P + + + + P  D        VR  +   ++I +   P P+ +W ++G +++         + Y        ++  S   D G YTL  EN +GS      + VL
Sbjct: 4547 WTPPKKDGGSPITSYIIEKRPRFGQW-------EKAAEIPGNQTSGRVPDLTEGQEYEFRVIAVNKGGPGEPSEASAPIVAKPRFIAPSFDTHLLHDLVVRAGQKISYNIPIVASPKPKATWNRDGVQIVADARH----EMYTTNTETSFEIPFSVRSDTGRYTLTLENEHGSFSASARVTVL 4718

HSP 12 Score: 94.3597 bits (233), Expect = 1.580e-16
Identity = 64/183 (34.97%), Postives = 90/183 (49.18%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            W++P+ DGG PIT YII+KKDK+   W  AL T            +A V GL EG  YQFRV+A NKAG S P   +K    + + L P ID+      ++       +   V GEPPP   W + G   ++SD  V   +       ++  V R    D+G YT+ A N +G D + + + V
Sbjct: 3661 WKEPVSDGGSPITGYIIEKKDKYSTMWEKALETGTPVT-------KALVHGLIEGNDYQFRVIAVNKAGQSEPGEASKTFTAKPRFLAPKIDRRNLRDVTLSAGSMLKFDANVIGEPPPHIEW-RYGAIPLHSDRKVQIDNIDYNTKFSIRPVSRD---DSGDYTVTASNSSGKDSVTVQVTV 3832

HSP 13 Score: 92.4337 bits (228), Expect = 5.840e-16
Identity = 60/187 (32.09%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVK--GEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT YI+QKK+K    W +A+       + T       VP L+EG+ Y+FRV+A N AG S PS  +     + + L P I       + VR+    I+H+ +   GEP P+ SW    N+L ++D   I +  Y      ++ +  ++  D+G Y L+ +N +G D+    + VL
Sbjct: 4840 QWPVPKNDGGSPITGYIVQKKEKGSPYWVNAVHVPAGQNSTT-------VPDLTEGQDYEFRVIAVNAAGQSEPSEPSDLVTAKPRFLAPKIKTPL---QDVRIKAGLIFHVDIDFIGEPTPEASWSVGSNELTSNDRTTITSIGYH----TIVHIVNAKRSDSGLYHLLLKNSSGIDEGSFQVTVL 5012

HSP 14 Score: 75.485 bits (184), Expect = 1.360e-10
Identity = 58/184 (31.52%), Postives = 76/184 (41.30%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG  I  YIIQKK K    W D          A I      VP L EG+ Y FRV A N  G S PS  + P +   +  +P +D G     +VR       H+   G P P  +W+ N    +  DE      +      A L V  ++  D G Y L   N +G D   +++ VL
Sbjct: 5536 WRSPRSDGGSKIRGYIIQKKAKGDDDWSDV-------NGAPIPSNVYTVPNLKEGEEYLFRVFAVNDVGSSDPSRPSNPIVIEEQPNKPVMDLGGVRDITVRAGEDFSIHVPYIGFPKPTATWFAND---VIKDETDPRVHQQLADDFASLVVKNAKRSDGGQYRLQLRNSSGFDTATVNVKVL 5709

HSP 15 Score: 67.3958 bits (163), Expect = 5.748e-8
Identity = 33/88 (37.50%), Postives = 47/88 (53.41%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W  P++ GG+PIT Y ++K+++ G W           T T+ DL        EG  Y+FRV A N+AG   PS  T+P +  H+ LRP
Sbjct: 5436 WNPPINTGGKPITGYYVEKRERGGEWLKVNNYPTPNTTFTVQDLH-------EGSRYEFRVSAVNEAGPGKPSKPTEPIIAGHQRLRP 5516

HSP 16 Score: 65.0846 bits (157), Expect = 2.680e-7
Identity = 54/234 (23.08%), Postives = 102/234 (43.59%), Query Frame = 0 
Query:  211 RLELNGLLGQLGEELLAEEHVLSRLQLLLPMHLGR--------------WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKV--KGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            R+    L G+  E L  +  V+++ Q ++P   G+              W  P+ +GG  I  Y ++++D+  G +     +        DD       ++EG  Y++RV A N AG    S  +     +    +P +   A   + ++V      ++ +   G P P+  W K+G  L+   E +  + E +   T +L + +S   D G YT+ A N +G D  D++++V+
Sbjct: 5091 RVSAENLQGR-SEPLTTDRSVVAKNQFVVPGQPGKPECVDADKDHIKIKWTPPISNGGSKIIGYDVERRDRATGRWIKQNKEPVRYPEYYDD------HVTEGHQYEYRVSAINAAGAGKASETSSVFTAKPMKEKPKLYLDALIGRKIKVRAGEPINVNIPLSGAPTPKIEWTKDGRSLM---ETLRLSTETKSDRTQLL-IEKSVRDDGGMYTVTATNEHGKDSADIEVIVV 5313

HSP 17 Score: 63.5438 bits (153), Expect = 7.362e-7
Identity = 52/187 (27.81%), Postives = 80/187 (42.78%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDD---KACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKG-AAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG PI  Y+I+K+         L+ +D   KA  A + D   KV GL E   Y+FRV A N AG  P SS +           P I    +    +V         +     P P+ +W  NG +++  +     T +    A+  +   +++  D GTYT+   N  G+D     ++V+
Sbjct: 5832 WTKPRHDGGSPILGYVIEKR---------LLNEDKWVKATPALVHDTTYKVTGLIENHDYEFRVAAENAAGRGPWSSNSDVIRASAAPFPPKITSDLSIRDMTVIAGEPFTITVPYTANPKPRPNWSINGEEVLTGERIKFETTDV---ASQFINK-KAKRSDTGTYTIYLTNTVGTDSASCKVLVV 6005
[Close]
BLAST of low quality protein: twitchin-like vs. nr
Match: gi|1068388462|ref|XP_018043882.1| (PREDICTED: twitchin isoform X17 [Atta colombica])

HSP 1 Score: 151.369 bits (381), Expect = 2.854e-35
Identity = 82/190 (43.16%), Postives = 107/190 (56.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMD 432
            +WEKP +DGGRPITHY ++ KDKF   W + + T D          EAKV GL E   YQFRV A NKAG S PS  T  HLC+H+NLRP ID+    S  ++  R+  W + V GEPPP+  W +++   L  ++   I   EY    T    +  +   D G YTLVAEN NG D+  ++L VL + D
Sbjct: 1610 KWEKPQNDGGRPITHYTVEIKDKFAVDWVEVMKTTDSTP-------EAKVEGLKEKMVYQFRVRAHNKAGSSEPSEPTDNHLCKHRNLRPRIDRTNFKSVIIKAGRTHKWSVDVSGEPPPEIRWIWRDNIPLTTTERIKIENVEYHTDFT----IVNAMRKDTGKYTLVAENANGKDEETVELTVLGKPD 1788

HSP 2 Score: 124.79 bits (312), Expect = 2.039e-26
Identity = 67/186 (36.02%), Postives = 102/186 (54.84%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            +W  P  DGG PI  YII+KKD++   W       +KA     + LE KVP L E   YQFRV+A NKAG SPPS  +K  +C+HK L+P ID+       VR  ++  + + V+GEPPP+  WY  GN++ + +   I   +Y       L +  S   + G + + A N +G D+ ++++ +L+
Sbjct: 1910 KWAPPEFDGGAPIEKYIIEKKDRYKPDW-------EKAIEVPGNQLEGKVPDLKERGEYQFRVIAVNKAGPSPPSDASKMQICKHKALKPRIDRTNLKPIIVRAGKTVKYDVDVRGEPPPEIKWYHVGNEVKSGENIEIINIDYN----TKLTITDSLRKNTGVWKIKAVNPHGEDEAEVEVTILS 2084

HSP 3 Score: 116.701 bits (291), Expect = 9.696e-24
Identity = 67/187 (35.83%), Postives = 100/187 (53.48%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFD-ALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            W  P DDGG PI  YI+Q +DK G  W D A V+ D+      D       G+ EG  Y+FR+VA NKAG   PS  +K  + + + L P ID+     K +R+ +       +KGEPPP  +W      L + D   I  ++Y      M  + + Q  D GTYT++A+N +G+D++D++L VL++
Sbjct: 2798 WTPPKDDGGAPIEKYIVQMRDKEGRQWVDVAKVSGDRTAAKVTD-------GIQEGHEYEFRIVAVNKAGPGEPSDTSKSVVAKPRFLAPHIDRKNLHKKIMRIGQMLRLEADIKGEPPPIVTWKLKDKVLKSMDRLKIENEDYH----TMFILNKLQRSDTGTYTVIAKNDSGTDQVDVELQVLSK 2973

HSP 4 Score: 116.316 bits (290), Expect = 1.240e-23
Identity = 67/185 (36.22%), Postives = 98/185 (52.97%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +WE P D GG PIT YII+KK+K    W +ALVTD           + +V GL +G  YQFR+ A NKAG S PS  TKPH+ + + L+P I++    +  VR  +     + V+GEPPP  +W   G  L       I  ++Y       +Q+  +     G YT+ A N +G D+ D+++ +L
Sbjct: 2205 KWEPPKDTGGAPITGYIIEKKEKPSSQWEEALVTDSP-------QPKGRVTGLKKGSTYQFRIRAVNKAGPSEPSDPTKPHVAKARFLKPHINRDKLQTIKVRAGQLVKLEVDVEGEPPPTVTWSFAGAPLSTGANIKIDNEDY----LTKIQLINTSRKLTGKYTIKAVNDSGQDEADVEINIL 2378

HSP 5 Score: 115.931 bits (289), Expect = 1.345e-23
Identity = 65/187 (34.76%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWF-DALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            +WE P+ DGG PIT YII+ KDKFG  F  A       CT T       VP L EG  YQFR+ A NKAG   PS  T PH  + + L+P ID+      +V+V  +    + + GEPPP+ +W     +L++ D   I   +Y         + +++    G Y + A+N  G D  ++++ VL +
Sbjct: 2502 KWEPPIRDGGAPITGYIIEMKDKFGTNFVKAAEVQGPVCTGT-------VPRLEEGNQYQFRIRAVNKAGPGEPSEATNPHTAKARWLKPYIDRTNLQPITVKVGLTVSLDVNIIGEPPPKVTWTFKDKELVSDDTIRIDNVDYN----TKFFILKTKRAHTGKYVINAKNEVGEDTAEVEITVLGK 2677

HSP 6 Score: 115.931 bits (289), Expect = 1.629e-23
Identity = 66/184 (35.87%), Postives = 99/184 (53.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+ P  DGG PI  YII+KKD++G W        KA     +  EAKVP L EG+ YQFRV A NK G S PS  ++  + + +   P ID+      +++        +KV GEPPP  +W+ N  K  + +   I T++Y+   T  +    ++ H  GT TL AEN +G D+  ++++VL
Sbjct: 3093 KWKAPKKDGGAPIEKYIIEKKDQYGKW-------QKAAEVPGNKTEAKVPDLVEGQKYQFRVKAVNKGGQSKPSEPSETLIAKDRYAAPKIDRTNLKDITIKAGSVIRLDVKVTGEPPPSKTWFLNKAKQESGNVLTIETEDYK---TKFVVSSATRAH-CGTLTLKAENSSGKDEASIEILVL 3265

HSP 7 Score: 114.39 bits (285), Expect = 4.804e-23
Identity = 66/184 (35.87%), Postives = 96/184 (52.17%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT Y+I+KKDK+G W       +K      D+  A VP L EG+ Y+FRV A NKAG   PS  T   + + +NL P ID+       ++  ++  +  KV GEPPP   W  NG ++  S+   +   +Y       L V  +   ++G YT+ AEN NG D+  + + VL
Sbjct: 3976 KWTPPENDGGSPITGYVIEKKDKYGEW-------EKTLEVPADETFATVPDLIEGQPYEFRVRAVNKAGPGEPSDATPTIIAKPRNLAPRIDRTNLIEVKIKAGQNFNFDCKVTGEPPPTTKWMLNGKEVRPSERVKVKHVDYN----TSLNVRMATRAESGKYTITAENINGRDEAFVKVTVL 4148

HSP 8 Score: 114.39 bits (285), Expect = 5.506e-23
Identity = 69/184 (37.50%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W +P +DGG PIT YII+K+DK+        T +K      D    +V  L EG  Y+FRV A NKAG    S  +K H+ R KNL P ID+       VR      + + V GEPPP   W    N ++ SD   I  ++Y       ++V  ++  D+G YTLVA+N NG D   L + VL
Sbjct: 4272 QWSRPEEDGGSPITGYIIEKRDKYSP------TWEKCAEIDGDTNRGRVDDLVEGTPYEFRVRAVNKAGPGEASEASKSHIARPKNLPPKIDRKYMLDVKVRAGSFFDFDVPVTGEPPPSKEWSLKDNVVMASDRIKIINEDYN----TKVRVMEAKRSDSGIYTLVAKNVNGKDSATLTVNVL 4445

HSP 9 Score: 103.219 bits (256), Expect = 2.412e-19
Identity = 64/184 (34.78%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP +DGG PIT YII+ K+KFG  W      +     ATID       GL EG  Y+FR+ A NKAG   PS  TKP + + + ++P I      +  V+  +   + IK  GEP P+  W+    ++I      +  D+Y+     +L V ++   D+G Y LV  N +G+ +   D+VVL
Sbjct: 1312 WTKPENDGGAPITGYIIEYKEKFGKEWVTGKEIEGDVTQATID-------GLKEGTQYEFRIRAVNKAGPGEPSDVTKPIIAKCRFVKPYIVGDGLTNLVVKKGQVIKYDIKYAGEPEPEVHWFLGQKEIIQDSAERVTIDKYE--RNTVLTVRKTTRPDSGKYKLVLTNSSGTCESVADVVVL 1486

HSP 10 Score: 100.908 bits (250), Expect = 1.090e-18
Identity = 63/189 (33.33%), Postives = 97/189 (51.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVF--RSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            RW  PL DGG PIT Y+I+K++K    W        KAC  T  D + +V  L EG  Y+FRV A N AG   PS  +KP   + + L P ID+      +VR + +  + +K+ GEPPP  +W  N   +  +    I    Y        + F  + +  D+GTY + A N++GSD  ++++ V+++
Sbjct: 3387 RWTPPLKDGGSPITGYVIEKREKGAPKWI-------KACE-TGPDCKGRVDNLDEGVEYEFRVKAINAAGLGEPSDASKPITAKSRRLPPKIDRRNLRDITVRENEAFHFDVKIIGEPPPDVTWVINNKSIQQTTHRRIQNVPYNS------KFFNDKPERKDSGTYKITAVNQHGSDTAEVEVTVVSK 3561

HSP 11 Score: 96.6709 bits (239), Expect = 2.854e-17
Identity = 55/183 (30.05%), Postives = 86/183 (46.99%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG PIT YII+K+ +FG W       +KA     +    +VP L+EG+ Y+FRV+A NK G   PS  + P + + + + P  D        VR  +   ++I +   P P+ +W ++G +++         + Y        ++  S   D G YTL  EN +GS      + VL
Sbjct: 4568 WTPPKKDGGSPITSYIIEKRPRFGQW-------EKAAEIPGNQTSGRVPDLTEGQEYEFRVIAVNKGGPGEPSEASAPIVAKPRFIAPSFDTHLLHDLVVRAGQKISYNIPIVASPKPKATWNRDGVQIVADARH----EMYTTNTETSFEIPFSVRSDTGRYTLTLENEHGSFSASARVTVL 4739

HSP 12 Score: 94.3597 bits (233), Expect = 1.580e-16
Identity = 64/183 (34.97%), Postives = 90/183 (49.18%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            W++P+ DGG PIT YII+KKDK+   W  AL T            +A V GL EG  YQFRV+A NKAG S P   +K    + + L P ID+      ++       +   V GEPPP   W + G   ++SD  V   +       ++  V R    D+G YT+ A N +G D + + + V
Sbjct: 3682 WKEPVSDGGSPITGYIIEKKDKYSTMWEKALETGTPVT-------KALVHGLIEGNDYQFRVIAVNKAGQSEPGEASKTFTAKPRFLAPKIDRRNLRDVTLSAGSMLKFDANVIGEPPPHIEW-RYGAIPLHSDRKVQIDNIDYNTKFSIRPVSRD---DSGDYTVTASNSSGKDSVTVQVTV 3853

HSP 13 Score: 92.4337 bits (228), Expect = 5.841e-16
Identity = 60/187 (32.09%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVK--GEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT YI+QKK+K    W +A+       + T       VP L+EG+ Y+FRV+A N AG S PS  +     + + L P I       + VR+    I+H+ +   GEP P+ SW    N+L ++D   I +  Y      ++ +  ++  D+G Y L+ +N +G D+    + VL
Sbjct: 4861 QWPVPKNDGGSPITGYIVQKKEKGSPYWVNAVHVPAGQNSTT-------VPDLTEGQDYEFRVIAVNAAGQSEPSEPSDLVTAKPRFLAPKIKTPL---QDVRIKAGLIFHVDIDFIGEPTPEASWSVGSNELTSNDRTTITSIGYH----TIVHIVNAKRSDSGLYHLLLKNSSGIDEGSFQVTVL 5033

HSP 14 Score: 75.485 bits (184), Expect = 1.372e-10
Identity = 58/184 (31.52%), Postives = 76/184 (41.30%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG  I  YIIQKK K    W D          A I      VP L EG+ Y FRV A N  G S PS  + P +   +  +P +D G     +VR       H+   G P P  +W+ N    +  DE      +      A L V  ++  D G Y L   N +G D   +++ VL
Sbjct: 5557 WRSPRSDGGSKIRGYIIQKKAKGDDDWSDV-------NGAPIPSNVYTVPNLKEGEEYLFRVFAVNDVGSSDPSRPSNPIVIEEQPNKPVMDLGGVRDITVRAGEDFSIHVPYIGFPKPTATWFAND---VIKDETDPRVHQQLADDFASLVVKNAKRSDGGQYRLQLRNSSGFDTATVNVKVL 5730

HSP 15 Score: 67.3958 bits (163), Expect = 5.749e-8
Identity = 33/88 (37.50%), Postives = 47/88 (53.41%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W  P++ GG+PIT Y ++K+++ G W           T T+ DL        EG  Y+FRV A N+AG   PS  T+P +  H+ LRP
Sbjct: 5457 WNPPINTGGKPITGYYVEKRERGGEWLKVNNYPTPNTTFTVQDLH-------EGSRYEFRVSAVNEAGPGKPSKPTEPIIAGHQRLRP 5537

HSP 16 Score: 65.0846 bits (157), Expect = 2.703e-7
Identity = 54/234 (23.08%), Postives = 102/234 (43.59%), Query Frame = 0 
Query:  211 RLELNGLLGQLGEELLAEEHVLSRLQLLLPMHLGR--------------WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKV--KGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            R+    L G+  E L  +  V+++ Q ++P   G+              W  P+ +GG  I  Y ++++D+  G +     +        DD       ++EG  Y++RV A N AG    S  +     +    +P +   A   + ++V      ++ +   G P P+  W K+G  L+   E +  + E +   T +L + +S   D G YT+ A N +G D  D++++V+
Sbjct: 5112 RVSAENLQGR-SEPLTTDRSVVAKNQFVVPGQPGKPECVDADKDHIKIKWTPPISNGGSKIIGYDVERRDRATGRWIKQNKEPVRYPEYYDD------HVTEGHQYEYRVSAINAAGAGKASETSSVFTAKPMKEKPKLYLDALIGRKIKVRAGEPINVNIPLSGAPTPKIEWTKDGRSLM---ETLRLSTETKSDRTQLL-IEKSVRDDGGMYTVTATNEHGKDSADIEVIVV 5334

HSP 17 Score: 63.5438 bits (153), Expect = 7.362e-7
Identity = 52/187 (27.81%), Postives = 80/187 (42.78%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDD---KACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKG-AAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG PI  Y+I+K+         L+ +D   KA  A + D   KV GL E   Y+FRV A N AG  P SS +           P I    +    +V         +     P P+ +W  NG +++  +     T +    A+  +   +++  D GTYT+   N  G+D     ++V+
Sbjct: 5853 WTKPRHDGGSPILGYVIEKR---------LLNEDKWVKATPALVHDTTYKVTGLIENHDYEFRVAAENAAGRGPWSSNSDVIRASAAPFPPKITSDLSIRDMTVIAGEPFTITVPYTANPKPRPNWSINGEEVLTGERIKFETTDV---ASQFINK-KAKRSDTGTYTIYLTNTVGTDSASCKVLVV 6026
[Close]
BLAST of low quality protein: twitchin-like vs. nr
Match: gi|1009363002|gb|KYM88060.1| (Titin [Atta colombica])

HSP 1 Score: 151.369 bits (381), Expect = 2.875e-35
Identity = 82/190 (43.16%), Postives = 107/190 (56.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMD 432
            +WEKP +DGGRPITHY ++ KDKF   W + + T D          EAKV GL E   YQFRV A NKAG S PS  T  HLC+H+NLRP ID+    S  ++  R+  W + V GEPPP+  W +++   L  ++   I   EY    T    +  +   D G YTLVAEN NG D+  ++L VL + D
Sbjct: 3054 KWEKPQNDGGRPITHYTVEIKDKFAVDWVEVMKTTDSTP-------EAKVEGLKEKMVYQFRVRAHNKAGSSEPSEPTDNHLCKHRNLRPRIDRTNFKSVIIKAGRTHKWSVDVSGEPPPEIRWIWRDNIPLTTTERIKIENVEYHTDFT----IVNAMRKDTGKYTLVAENANGKDEETVELTVLGKPD 3232

HSP 2 Score: 124.79 bits (312), Expect = 2.073e-26
Identity = 67/186 (36.02%), Postives = 102/186 (54.84%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            +W  P  DGG PI  YII+KKD++   W       +KA     + LE KVP L E   YQFRV+A NKAG SPPS  +K  +C+HK L+P ID+       VR  ++  + + V+GEPPP+  WY  GN++ + +   I   +Y       L +  S   + G + + A N +G D+ ++++ +L+
Sbjct: 3354 KWAPPEFDGGAPIEKYIIEKKDRYKPDW-------EKAIEVPGNQLEGKVPDLKERGEYQFRVIAVNKAGPSPPSDASKMQICKHKALKPRIDRTNLKPIIVRAGKTVKYDVDVRGEPPPEIKWYHVGNEVKSGENIEIINIDYN----TKLTITDSLRKNTGVWKIKAVNPHGEDEAEVEVTILS 3528

HSP 3 Score: 116.701 bits (291), Expect = 9.765e-24
Identity = 67/187 (35.83%), Postives = 100/187 (53.48%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFD-ALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            W  P DDGG PI  YI+Q +DK G  W D A V+ D+      D       G+ EG  Y+FR+VA NKAG   PS  +K  + + + L P ID+     K +R+ +       +KGEPPP  +W      L + D   I  ++Y      M  + + Q  D GTYT++A+N +G+D++D++L VL++
Sbjct: 4242 WTPPKDDGGAPIEKYIVQMRDKEGRQWVDVAKVSGDRTAAKVTD-------GIQEGHEYEFRIVAVNKAGPGEPSDTSKSVVAKPRFLAPHIDRKNLHKKIMRIGQMLRLEADIKGEPPPIVTWKLKDKVLKSMDRLKIENEDYH----TMFILNKLQRSDTGTYTVIAKNDSGTDQVDVELQVLSK 4417

HSP 4 Score: 116.316 bits (290), Expect = 1.271e-23
Identity = 67/185 (36.22%), Postives = 98/185 (52.97%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +WE P D GG PIT YII+KK+K    W +ALVTD           + +V GL +G  YQFR+ A NKAG S PS  TKPH+ + + L+P I++    +  VR  +     + V+GEPPP  +W   G  L       I  ++Y       +Q+  +     G YT+ A N +G D+ D+++ +L
Sbjct: 3649 KWEPPKDTGGAPITGYIIEKKEKPSSQWEEALVTDSP-------QPKGRVTGLKKGSTYQFRIRAVNKAGPSEPSDPTKPHVAKARFLKPHINRDKLQTIKVRAGQLVKLEVDVEGEPPPTVTWSFAGAPLSTGANIKIDNEDY----LTKIQLINTSRKLTGKYTIKAVNDSGQDEADVEINIL 3822

HSP 5 Score: 115.931 bits (289), Expect = 1.380e-23
Identity = 65/187 (34.76%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWF-DALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            +WE P+ DGG PIT YII+ KDKFG  F  A       CT T       VP L EG  YQFR+ A NKAG   PS  T PH  + + L+P ID+      +V+V  +    + + GEPPP+ +W     +L++ D   I   +Y         + +++    G Y + A+N  G D  ++++ VL +
Sbjct: 3946 KWEPPIRDGGAPITGYIIEMKDKFGTNFVKAAEVQGPVCTGT-------VPRLEEGNQYQFRIRAVNKAGPGEPSEATNPHTAKARWLKPYIDRTNLQPITVKVGLTVSLDVNIIGEPPPKVTWTFKDKELVSDDTIRIDNVDYN----TKFFILKTKRAHTGKYVINAKNEVGEDTAEVEITVLGK 4121

HSP 6 Score: 115.931 bits (289), Expect = 1.640e-23
Identity = 66/184 (35.87%), Postives = 99/184 (53.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+ P  DGG PI  YII+KKD++G W        KA     +  EAKVP L EG+ YQFRV A NK G S PS  ++  + + +   P ID+      +++        +KV GEPPP  +W+ N  K  + +   I T++Y+   T  +    ++ H  GT TL AEN +G D+  ++++VL
Sbjct: 4537 KWKAPKKDGGAPIEKYIIEKKDQYGKW-------QKAAEVPGNKTEAKVPDLVEGQKYQFRVKAVNKGGQSKPSEPSETLIAKDRYAAPKIDRTNLKDITIKAGSVIRLDVKVTGEPPPSKTWFLNKAKQESGNVLTIETEDYK---TKFVVSSATRAH-CGTLTLKAENSSGKDEASIEILVL 4709

HSP 7 Score: 114.39 bits (285), Expect = 4.882e-23
Identity = 66/184 (35.87%), Postives = 96/184 (52.17%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT Y+I+KKDK+G W       +K      D+  A VP L EG+ Y+FRV A NKAG   PS  T   + + +NL P ID+       ++  ++  +  KV GEPPP   W  NG ++  S+   +   +Y       L V  +   ++G YT+ AEN NG D+  + + VL
Sbjct: 5420 KWTPPENDGGSPITGYVIEKKDKYGEW-------EKTLEVPADETFATVPDLIEGQPYEFRVRAVNKAGPGEPSDATPTIIAKPRNLAPRIDRTNLIEVKIKAGQNFNFDCKVTGEPPPTTKWMLNGKEVRPSERVKVKHVDYN----TSLNVRMATRAESGKYTITAENINGRDEAFVKVTVL 5592

HSP 8 Score: 114.39 bits (285), Expect = 5.544e-23
Identity = 69/184 (37.50%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W +P +DGG PIT YII+K+DK+        T +K      D    +V  L EG  Y+FRV A NKAG    S  +K H+ R KNL P ID+       VR      + + V GEPPP   W    N ++ SD   I  ++Y       ++V  ++  D+G YTLVA+N NG D   L + VL
Sbjct: 5716 QWSRPEEDGGSPITGYIIEKRDKYSP------TWEKCAEIDGDTNRGRVDDLVEGTPYEFRVRAVNKAGPGEASEASKSHIARPKNLPPKIDRKYMLDVKVRAGSFFDFDVPVTGEPPPSKEWSLKDNVVMASDRIKIINEDYN----TKVRVMEAKRSDSGIYTLVAKNVNGKDSATLTVNVL 5889

HSP 9 Score: 103.219 bits (256), Expect = 2.428e-19
Identity = 64/184 (34.78%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP +DGG PIT YII+ K+KFG  W      +     ATID       GL EG  Y+FR+ A NKAG   PS  TKP + + + ++P I      +  V+  +   + IK  GEP P+  W+    ++I      +  D+Y+     +L V ++   D+G Y LV  N +G+ +   D+VVL
Sbjct: 2756 WTKPENDGGAPITGYIIEYKEKFGKEWVTGKEIEGDVTQATID-------GLKEGTQYEFRIRAVNKAGPGEPSDVTKPIIAKCRFVKPYIVGDGLTNLVVKKGQVIKYDIKYAGEPEPEVHWFLGQKEIIQDSAERVTIDKYE--RNTVLTVRKTTRPDSGKYKLVLTNSSGTCESVADVVVL 2930

HSP 10 Score: 100.908 bits (250), Expect = 1.097e-18
Identity = 63/189 (33.33%), Postives = 97/189 (51.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVF--RSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            RW  PL DGG PIT Y+I+K++K    W        KAC  T  D + +V  L EG  Y+FRV A N AG   PS  +KP   + + L P ID+      +VR + +  + +K+ GEPPP  +W  N   +  +    I    Y        + F  + +  D+GTY + A N++GSD  ++++ V+++
Sbjct: 4831 RWTPPLKDGGSPITGYVIEKREKGAPKWI-------KACE-TGPDCKGRVDNLDEGVEYEFRVKAINAAGLGEPSDASKPITAKSRRLPPKIDRRNLRDITVRENEAFHFDVKIIGEPPPDVTWVINNKSIQQTTHRRIQNVPYNS------KFFNDKPERKDSGTYKITAVNQHGSDTAEVEVTVVSK 5005

HSP 11 Score: 96.6709 bits (239), Expect = 2.898e-17
Identity = 55/183 (30.05%), Postives = 86/183 (46.99%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG PIT YII+K+ +FG W       +KA     +    +VP L+EG+ Y+FRV+A NK G   PS  + P + + + + P  D        VR  +   ++I +   P P+ +W ++G +++         + Y        ++  S   D G YTL  EN +GS      + VL
Sbjct: 6012 WTPPKKDGGSPITSYIIEKRPRFGQW-------EKAAEIPGNQTSGRVPDLTEGQEYEFRVIAVNKGGPGEPSEASAPIVAKPRFIAPSFDTHLLHDLVVRAGQKISYNIPIVASPKPKATWNRDGVQIVADARH----EMYTTNTETSFEIPFSVRSDTGRYTLTLENEHGSFSASARVTVL 6183

HSP 12 Score: 94.3597 bits (233), Expect = 1.619e-16
Identity = 64/183 (34.97%), Postives = 90/183 (49.18%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            W++P+ DGG PIT YII+KKDK+   W  AL T            +A V GL EG  YQFRV+A NKAG S P   +K    + + L P ID+      ++       +   V GEPPP   W + G   ++SD  V   +       ++  V R    D+G YT+ A N +G D + + + V
Sbjct: 5126 WKEPVSDGGSPITGYIIEKKDKYSTMWEKALETGTPVT-------KALVHGLIEGNDYQFRVIAVNKAGQSEPGEASKTFTAKPRFLAPKIDRRNLRDVTLSAGSMLKFDANVIGEPPPHIEW-RYGAIPLHSDRKVQIDNIDYNTKFSIRPVSRD---DSGDYTVTASNSSGKDSVTVQVTV 5297

HSP 13 Score: 92.4337 bits (228), Expect = 5.983e-16
Identity = 60/187 (32.09%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVK--GEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT YI+QKK+K    W +A+       + T       VP L+EG+ Y+FRV+A N AG S PS  +     + + L P I       + VR+    I+H+ +   GEP P+ SW    N+L ++D   I +  Y      ++ +  ++  D+G Y L+ +N +G D+    + VL
Sbjct: 6305 QWPVPKNDGGSPITGYIVQKKEKGSPYWVNAVHVPAGQNSTT-------VPDLTEGQDYEFRVIAVNAAGQSEPSEPSDLVTAKPRFLAPKIKTPL---QDVRIKAGLIFHVDIDFIGEPTPEASWSVGSNELTSNDRTTITSIGYH----TIVHIVNAKRSDSGLYHLLLKNSSGIDEGSFQVTVL 6477

HSP 14 Score: 75.485 bits (184), Expect = 1.428e-10
Identity = 58/184 (31.52%), Postives = 76/184 (41.30%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG  I  YIIQKK K    W D          A I      VP L EG+ Y FRV A N  G S PS  + P +   +  +P +D G     +VR       H+   G P P  +W+ N    +  DE      +      A L V  ++  D G Y L   N +G D   +++ VL
Sbjct: 7001 WRSPRSDGGSKIRGYIIQKKAKGDDDWSDV-------NGAPIPSNVYTVPNLKEGEEYLFRVFAVNDVGSSDPSRPSNPIVIEEQPNKPVMDLGGVRDITVRAGEDFSIHVPYIGFPKPTATWFAND---VIKDETDPRVHQQLADDFASLVVKNAKRSDGGQYRLQLRNSSGFDTATVNVKVL 7174

HSP 15 Score: 67.3958 bits (163), Expect = 5.827e-8
Identity = 33/88 (37.50%), Postives = 47/88 (53.41%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W  P++ GG+PIT Y ++K+++ G W           T T+ DL        EG  Y+FRV A N+AG   PS  T+P +  H+ LRP
Sbjct: 6901 WNPPINTGGKPITGYYVEKRERGGEWLKVNNYPTPNTTFTVQDLH-------EGSRYEFRVSAVNEAGPGKPSKPTEPIIAGHQRLRP 6981

HSP 16 Score: 65.0846 bits (157), Expect = 2.836e-7
Identity = 54/234 (23.08%), Postives = 102/234 (43.59%), Query Frame = 0 
Query:  211 RLELNGLLGQLGEELLAEEHVLSRLQLLLPMHLGR--------------WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKV--KGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            R+    L G+  E L  +  V+++ Q ++P   G+              W  P+ +GG  I  Y ++++D+  G +     +        DD       ++EG  Y++RV A N AG    S  +     +    +P +   A   + ++V      ++ +   G P P+  W K+G  L+   E +  + E +   T +L + +S   D G YT+ A N +G D  D++++V+
Sbjct: 6556 RVSAENLQGR-SEPLTTDRSVVAKNQFVVPGQPGKPECVDADKDHIKIKWTPPISNGGSKIIGYDVERRDRATGRWIKQNKEPVRYPEYYDD------HVTEGHQYEYRVSAINAAGAGKASETSSVFTAKPMKEKPKLYLDALIGRKIKVRAGEPINVNIPLSGAPTPKIEWTKDGRSLM---ETLRLSTETKSDRTQLL-IEKSVRDDGGMYTVTATNEHGKDSADIEVIVV 6778

HSP 17 Score: 63.5438 bits (153), Expect = 7.397e-7
Identity = 52/187 (27.81%), Postives = 80/187 (42.78%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDD---KACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKG-AAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG PI  Y+I+K+         L+ +D   KA  A + D   KV GL E   Y+FRV A N AG  P SS +           P I    +    +V         +     P P+ +W  NG +++  +     T +    A+  +   +++  D GTYT+   N  G+D     ++V+
Sbjct: 7297 WTKPRHDGGSPILGYVIEKR---------LLNEDKWVKATPALVHDTTYKVTGLIENHDYEFRVAAENAAGRGPWSSNSDVIRASAAPFPPKITSDLSIRDMTVIAGEPFTITVPYTANPKPRPNWSINGEEVLTGERIKFETTDV---ASQFINK-KAKRSDTGTYTIYLTNTVGTDSASCKVLVV 7470
[Close]
BLAST of low quality protein: twitchin-like vs. nr
Match: gi|1068388479|ref|XP_018043888.1| (PREDICTED: twitchin isoform X22 [Atta colombica])

HSP 1 Score: 150.984 bits (380), Expect = 3.013e-35
Identity = 82/190 (43.16%), Postives = 107/190 (56.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMD 432
            +WEKP +DGGRPITHY ++ KDKF   W + + T D          EAKV GL E   YQFRV A NKAG S PS  T  HLC+H+NLRP ID+    S  ++  R+  W + V GEPPP+  W +++   L  ++   I   EY    T    +  +   D G YTLVAEN NG D+  ++L VL + D
Sbjct: 1398 KWEKPQNDGGRPITHYTVEIKDKFAVDWVEVMKTTDSTP-------EAKVEGLKEKMVYQFRVRAHNKAGSSEPSEPTDNHLCKHRNLRPRIDRTNFKSVIIKAGRTHKWSVDVSGEPPPEIRWIWRDNIPLTTTERIKIENVEYHTDFT----IVNAMRKDTGKYTLVAENANGKDEETVELTVLGKPD 1576

HSP 2 Score: 124.405 bits (311), Expect = 2.152e-26
Identity = 67/186 (36.02%), Postives = 102/186 (54.84%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            +W  P  DGG PI  YII+KKD++   W       +KA     + LE KVP L E   YQFRV+A NKAG SPPS  +K  +C+HK L+P ID+       VR  ++  + + V+GEPPP+  WY  GN++ + +   I   +Y       L +  S   + G + + A N +G D+ ++++ +L+
Sbjct: 1698 KWAPPEFDGGAPIEKYIIEKKDRYKPDW-------EKAIEVPGNQLEGKVPDLKERGEYQFRVIAVNKAGPSPPSDASKMQICKHKALKPRIDRTNLKPIIVRAGKTVKYDVDVRGEPPPEIKWYHVGNEVKSGENIEIINIDYN----TKLTITDSLRKNTGVWKIKAVNPHGEDEAEVEVTILS 1872

HSP 3 Score: 116.316 bits (290), Expect = 1.023e-23
Identity = 67/187 (35.83%), Postives = 100/187 (53.48%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFD-ALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            W  P DDGG PI  YI+Q +DK G  W D A V+ D+      D       G+ EG  Y+FR+VA NKAG   PS  +K  + + + L P ID+     K +R+ +       +KGEPPP  +W      L + D   I  ++Y      M  + + Q  D GTYT++A+N +G+D++D++L VL++
Sbjct: 2586 WTPPKDDGGAPIEKYIVQMRDKEGRQWVDVAKVSGDRTAAKVTD-------GIQEGHEYEFRIVAVNKAGPGEPSDTSKSVVAKPRFLAPHIDRKNLHKKIMRIGQMLRLEADIKGEPPPIVTWKLKDKVLKSMDRLKIENEDYH----TMFILNKLQRSDTGTYTVIAKNDSGTDQVDVELQVLSK 2761

HSP 4 Score: 115.931 bits (289), Expect = 1.308e-23
Identity = 67/185 (36.22%), Postives = 98/185 (52.97%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +WE P D GG PIT YII+KK+K    W +ALVTD           + +V GL +G  YQFR+ A NKAG S PS  TKPH+ + + L+P I++    +  VR  +     + V+GEPPP  +W   G  L       I  ++Y       +Q+  +     G YT+ A N +G D+ D+++ +L
Sbjct: 1993 KWEPPKDTGGAPITGYIIEKKEKPSSQWEEALVTDSP-------QPKGRVTGLKKGSTYQFRIRAVNKAGPSEPSDPTKPHVAKARFLKPHINRDKLQTIKVRAGQLVKLEVDVEGEPPPTVTWSFAGAPLSTGANIKIDNEDY----LTKIQLINTSRKLTGKYTIKAVNDSGQDEADVEINIL 2166

HSP 5 Score: 115.931 bits (289), Expect = 1.432e-23
Identity = 65/187 (34.76%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWF-DALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            +WE P+ DGG PIT YII+ KDKFG  F  A       CT T       VP L EG  YQFR+ A NKAG   PS  T PH  + + L+P ID+      +V+V  +    + + GEPPP+ +W     +L++ D   I   +Y         + +++    G Y + A+N  G D  ++++ VL +
Sbjct: 2290 KWEPPIRDGGAPITGYIIEMKDKFGTNFVKAAEVQGPVCTGT-------VPRLEEGNQYQFRIRAVNKAGPGEPSEATNPHTAKARWLKPYIDRTNLQPITVKVGLTVSLDVNIIGEPPPKVTWTFKDKELVSDDTIRIDNVDYN----TKFFILKTKRAHTGKYVINAKNEVGEDTAEVEITVLGK 2465

HSP 6 Score: 115.931 bits (289), Expect = 1.687e-23
Identity = 66/184 (35.87%), Postives = 99/184 (53.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+ P  DGG PI  YII+KKD++G W        KA     +  EAKVP L EG+ YQFRV A NK G S PS  ++  + + +   P ID+      +++        +KV GEPPP  +W+ N  K  + +   I T++Y+   T  +    ++ H  GT TL AEN +G D+  ++++VL
Sbjct: 2881 KWKAPKKDGGAPIEKYIIEKKDQYGKW-------QKAAEVPGNKTEAKVPDLVEGQKYQFRVKAVNKGGQSKPSEPSETLIAKDRYAAPKIDRTNLKDITIKAGSVIRLDVKVTGEPPPSKTWFLNKAKQESGNVLTIETEDYK---TKFVVSSATRAH-CGTLTLKAENSSGKDEASIEILVL 3053

HSP 7 Score: 114.39 bits (285), Expect = 4.976e-23
Identity = 66/184 (35.87%), Postives = 96/184 (52.17%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT Y+I+KKDK+G W       +K      D+  A VP L EG+ Y+FRV A NKAG   PS  T   + + +NL P ID+       ++  ++  +  KV GEPPP   W  NG ++  S+   +   +Y       L V  +   ++G YT+ AEN NG D+  + + VL
Sbjct: 3764 KWTPPENDGGSPITGYVIEKKDKYGEW-------EKTLEVPADETFATVPDLIEGQPYEFRVRAVNKAGPGEPSDATPTIIAKPRNLAPRIDRTNLIEVKIKAGQNFNFDCKVTGEPPPTTKWMLNGKEVRPSERVKVKHVDYN----TSLNVRMATRAESGKYTITAENINGRDEAFVKVTVL 3936

HSP 8 Score: 114.005 bits (284), Expect = 5.651e-23
Identity = 69/184 (37.50%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W +P +DGG PIT YII+K+DK+        T +K      D    +V  L EG  Y+FRV A NKAG    S  +K H+ R KNL P ID+       VR      + + V GEPPP   W    N ++ SD   I  ++Y       ++V  ++  D+G YTLVA+N NG D   L + VL
Sbjct: 4060 QWSRPEEDGGSPITGYIIEKRDKYSP------TWEKCAEIDGDTNRGRVDDLVEGTPYEFRVRAVNKAGPGEASEASKSHIARPKNLPPKIDRKYMLDVKVRAGSFFDFDVPVTGEPPPSKEWSLKDNVVMASDRIKIINEDYN----TKVRVMEAKRSDSGIYTLVAKNVNGKDSATLTVNVL 4233

HSP 9 Score: 102.834 bits (255), Expect = 2.497e-19
Identity = 64/184 (34.78%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP +DGG PIT YII+ K+KFG  W      +     ATID       GL EG  Y+FR+ A NKAG   PS  TKP + + + ++P I      +  V+  +   + IK  GEP P+  W+    ++I      +  D+Y+     +L V ++   D+G Y LV  N +G+ +   D+VVL
Sbjct: 1100 WTKPENDGGAPITGYIIEYKEKFGKEWVTGKEIEGDVTQATID-------GLKEGTQYEFRIRAVNKAGPGEPSDVTKPIIAKCRFVKPYIVGDGLTNLVVKKGQVIKYDIKYAGEPEPEVHWFLGQKEIIQDSAERVTIDKYE--RNTVLTVRKTTRPDSGKYKLVLTNSSGTCESVADVVVL 1274

HSP 10 Score: 100.908 bits (250), Expect = 1.128e-18
Identity = 63/189 (33.33%), Postives = 97/189 (51.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVF--RSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            RW  PL DGG PIT Y+I+K++K    W        KAC  T  D + +V  L EG  Y+FRV A N AG   PS  +KP   + + L P ID+      +VR + +  + +K+ GEPPP  +W  N   +  +    I    Y        + F  + +  D+GTY + A N++GSD  ++++ V+++
Sbjct: 3175 RWTPPLKDGGSPITGYVIEKREKGAPKWI-------KACE-TGPDCKGRVDNLDEGVEYEFRVKAINAAGLGEPSDASKPITAKSRRLPPKIDRRNLRDITVRENEAFHFDVKIIGEPPPDVTWVINNKSIQQTTHRRIQNVPYNS------KFFNDKPERKDSGTYKITAVNQHGSDTAEVEVTVVSK 3349

HSP 11 Score: 96.6709 bits (239), Expect = 2.981e-17
Identity = 55/183 (30.05%), Postives = 86/183 (46.99%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG PIT YII+K+ +FG W       +KA     +    +VP L+EG+ Y+FRV+A NK G   PS  + P + + + + P  D        VR  +   ++I +   P P+ +W ++G +++         + Y        ++  S   D G YTL  EN +GS      + VL
Sbjct: 4356 WTPPKKDGGSPITSYIIEKRPRFGQW-------EKAAEIPGNQTSGRVPDLTEGQEYEFRVIAVNKGGPGEPSEASAPIVAKPRFIAPSFDTHLLHDLVVRAGQKISYNIPIVASPKPKATWNRDGVQIVADARH----EMYTTNTETSFEIPFSVRSDTGRYTLTLENEHGSFSASARVTVL 4527

HSP 12 Score: 94.3597 bits (233), Expect = 1.636e-16
Identity = 64/183 (34.97%), Postives = 90/183 (49.18%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            W++P+ DGG PIT YII+KKDK+   W  AL T            +A V GL EG  YQFRV+A NKAG S P   +K    + + L P ID+      ++       +   V GEPPP   W + G   ++SD  V   +       ++  V R    D+G YT+ A N +G D + + + V
Sbjct: 3470 WKEPVSDGGSPITGYIIEKKDKYSTMWEKALETGTPVT-------KALVHGLIEGNDYQFRVIAVNKAGQSEPGEASKTFTAKPRFLAPKIDRRNLRDVTLSAGSMLKFDANVIGEPPPHIEW-RYGAIPLHSDRKVQIDNIDYNTKFSIRPVSRD---DSGDYTVTASNSSGKDSVTVQVTV 3641

HSP 13 Score: 92.4337 bits (228), Expect = 5.939e-16
Identity = 60/187 (32.09%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVK--GEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT YI+QKK+K    W +A+       + T       VP L+EG+ Y+FRV+A N AG S PS  +     + + L P I       + VR+    I+H+ +   GEP P+ SW    N+L ++D   I +  Y      ++ +  ++  D+G Y L+ +N +G D+    + VL
Sbjct: 4649 QWPVPKNDGGSPITGYIVQKKEKGSPYWVNAVHVPAGQNSTT-------VPDLTEGQDYEFRVIAVNAAGQSEPSEPSDLVTAKPRFLAPKIKTPL---QDVRIKAGLIFHVDIDFIGEPTPEASWSVGSNELTSNDRTTITSIGYH----TIVHIVNAKRSDSGLYHLLLKNSSGIDEGSFQVTVL 4821

HSP 14 Score: 75.485 bits (184), Expect = 1.358e-10
Identity = 58/184 (31.52%), Postives = 76/184 (41.30%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG  I  YIIQKK K    W D          A I      VP L EG+ Y FRV A N  G S PS  + P +   +  +P +D G     +VR       H+   G P P  +W+ N    +  DE      +      A L V  ++  D G Y L   N +G D   +++ VL
Sbjct: 5345 WRSPRSDGGSKIRGYIIQKKAKGDDDWSDV-------NGAPIPSNVYTVPNLKEGEEYLFRVFAVNDVGSSDPSRPSNPIVIEEQPNKPVMDLGGVRDITVRAGEDFSIHVPYIGFPKPTATWFAND---VIKDETDPRVHQQLADDFASLVVKNAKRSDGGQYRLQLRNSSGFDTATVNVKVL 5518

HSP 15 Score: 67.3958 bits (163), Expect = 5.895e-8
Identity = 33/88 (37.50%), Postives = 47/88 (53.41%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W  P++ GG+PIT Y ++K+++ G W           T T+ DL        EG  Y+FRV A N+AG   PS  T+P +  H+ LRP
Sbjct: 5245 WNPPINTGGKPITGYYVEKRERGGEWLKVNNYPTPNTTFTVQDLH-------EGSRYEFRVSAVNEAGPGKPSKPTEPIIAGHQRLRP 5325

HSP 16 Score: 65.0846 bits (157), Expect = 2.894e-7
Identity = 54/234 (23.08%), Postives = 102/234 (43.59%), Query Frame = 0 
Query:  211 RLELNGLLGQLGEELLAEEHVLSRLQLLLPMHLGR--------------WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKV--KGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            R+    L G+  E L  +  V+++ Q ++P   G+              W  P+ +GG  I  Y ++++D+  G +     +        DD       ++EG  Y++RV A N AG    S  +     +    +P +   A   + ++V      ++ +   G P P+  W K+G  L+   E +  + E +   T +L + +S   D G YT+ A N +G D  D++++V+
Sbjct: 4900 RVSAENLQGR-SEPLTTDRSVVAKNQFVVPGQPGKPECVDADKDHIKIKWTPPISNGGSKIIGYDVERRDRATGRWIKQNKEPVRYPEYYDD------HVTEGHQYEYRVSAINAAGAGKASETSSVFTAKPMKEKPKLYLDALIGRKIKVRAGEPINVNIPLSGAPTPKIEWTKDGRSLM---ETLRLSTETKSDRTQLL-IEKSVRDDGGMYTVTATNEHGKDSADIEVIVV 5122

HSP 17 Score: 63.5438 bits (153), Expect = 7.356e-7
Identity = 52/187 (27.81%), Postives = 80/187 (42.78%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDD---KACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKG-AAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG PI  Y+I+K+         L+ +D   KA  A + D   KV GL E   Y+FRV A N AG  P SS +           P I    +    +V         +     P P+ +W  NG +++  +     T +    A+  +   +++  D GTYT+   N  G+D     ++V+
Sbjct: 5641 WTKPRHDGGSPILGYVIEKR---------LLNEDKWVKATPALVHDTTYKVTGLIENHDYEFRVAAENAAGRGPWSSNSDVIRASAAPFPPKITSDLSIRDMTVIAGEPFTITVPYTANPKPRPNWSINGEEVLTGERIKFETTDV---ASQFINK-KAKRSDTGTYTIYLTNTVGTDSASCKVLVV 5814
[Close]
BLAST of low quality protein: twitchin-like vs. nr
Match: gi|1068388468|ref|XP_018043884.1| (PREDICTED: twitchin isoform X19 [Atta colombica])

HSP 1 Score: 150.984 bits (380), Expect = 3.218e-35
Identity = 82/190 (43.16%), Postives = 107/190 (56.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSW-YKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQMD 432
            +WEKP +DGGRPITHY ++ KDKF   W + + T D          EAKV GL E   YQFRV A NKAG S PS  T  HLC+H+NLRP ID+    S  ++  R+  W + V GEPPP+  W +++   L  ++   I   EY    T    +  +   D G YTLVAEN NG D+  ++L VL + D
Sbjct: 1464 KWEKPQNDGGRPITHYTVEIKDKFAVDWVEVMKTTDSTP-------EAKVEGLKEKMVYQFRVRAHNKAGSSEPSEPTDNHLCKHRNLRPRIDRTNFKSVIIKAGRTHKWSVDVSGEPPPEIRWIWRDNIPLTTTERIKIENVEYHTDFT----IVNAMRKDTGKYTLVAENANGKDEETVELTVLGKPD 1642

HSP 2 Score: 124.405 bits (311), Expect = 2.233e-26
Identity = 67/186 (36.02%), Postives = 102/186 (54.84%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLA 429
            +W  P  DGG PI  YII+KKD++   W       +KA     + LE KVP L E   YQFRV+A NKAG SPPS  +K  +C+HK L+P ID+       VR  ++  + + V+GEPPP+  WY  GN++ + +   I   +Y       L +  S   + G + + A N +G D+ ++++ +L+
Sbjct: 1764 KWAPPEFDGGAPIEKYIIEKKDRYKPDW-------EKAIEVPGNQLEGKVPDLKERGEYQFRVIAVNKAGPSPPSDASKMQICKHKALKPRIDRTNLKPIIVRAGKTVKYDVDVRGEPPPEIKWYHVGNEVKSGENIEIINIDYN----TKLTITDSLRKNTGVWKIKAVNPHGEDEAEVEVTILS 1938

HSP 3 Score: 116.316 bits (290), Expect = 1.042e-23
Identity = 67/187 (35.83%), Postives = 100/187 (53.48%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFG-GWFD-ALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            W  P DDGG PI  YI+Q +DK G  W D A V+ D+      D       G+ EG  Y+FR+VA NKAG   PS  +K  + + + L P ID+     K +R+ +       +KGEPPP  +W      L + D   I  ++Y      M  + + Q  D GTYT++A+N +G+D++D++L VL++
Sbjct: 2652 WTPPKDDGGAPIEKYIVQMRDKEGRQWVDVAKVSGDRTAAKVTD-------GIQEGHEYEFRIVAVNKAGPGEPSDTSKSVVAKPRFLAPHIDRKNLHKKIMRIGQMLRLEADIKGEPPPIVTWKLKDKVLKSMDRLKIENEDYH----TMFILNKLQRSDTGTYTVIAKNDSGTDQVDVELQVLSK 2827

HSP 4 Score: 115.931 bits (289), Expect = 1.357e-23
Identity = 67/185 (36.22%), Postives = 98/185 (52.97%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +WE P D GG PIT YII+KK+K    W +ALVTD           + +V GL +G  YQFR+ A NKAG S PS  TKPH+ + + L+P I++    +  VR  +     + V+GEPPP  +W   G  L       I  ++Y       +Q+  +     G YT+ A N +G D+ D+++ +L
Sbjct: 2059 KWEPPKDTGGAPITGYIIEKKEKPSSQWEEALVTDSP-------QPKGRVTGLKKGSTYQFRIRAVNKAGPSEPSDPTKPHVAKARFLKPHINRDKLQTIKVRAGQLVKLEVDVEGEPPPTVTWSFAGAPLSTGANIKIDNEDY----LTKIQLINTSRKLTGKYTIKAVNDSGQDEADVEINIL 2232

HSP 5 Score: 115.931 bits (289), Expect = 1.486e-23
Identity = 65/187 (34.76%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWF-DALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            +WE P+ DGG PIT YII+ KDKFG  F  A       CT T       VP L EG  YQFR+ A NKAG   PS  T PH  + + L+P ID+      +V+V  +    + + GEPPP+ +W     +L++ D   I   +Y         + +++    G Y + A+N  G D  ++++ VL +
Sbjct: 2356 KWEPPIRDGGAPITGYIIEMKDKFGTNFVKAAEVQGPVCTGT-------VPRLEEGNQYQFRIRAVNKAGPGEPSEATNPHTAKARWLKPYIDRTNLQPITVKVGLTVSLDVNIIGEPPPKVTWTFKDKELVSDDTIRIDNVDYN----TKFFILKTKRAHTGKYVINAKNEVGEDTAEVEITVLGK 2531

HSP 6 Score: 115.931 bits (289), Expect = 1.734e-23
Identity = 66/184 (35.87%), Postives = 99/184 (53.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W+ P  DGG PI  YII+KKD++G W        KA     +  EAKVP L EG+ YQFRV A NK G S PS  ++  + + +   P ID+      +++        +KV GEPPP  +W+ N  K  + +   I T++Y+   T  +    ++ H  GT TL AEN +G D+  ++++VL
Sbjct: 2947 KWKAPKKDGGAPIEKYIIEKKDQYGKW-------QKAAEVPGNKTEAKVPDLVEGQKYQFRVKAVNKGGQSKPSEPSETLIAKDRYAAPKIDRTNLKDITIKAGSVIRLDVKVTGEPPPSKTWFLNKAKQESGNVLTIETEDYK---TKFVVSSATRAH-CGTLTLKAENSSGKDEASIEILVL 3119

HSP 7 Score: 114.39 bits (285), Expect = 5.115e-23
Identity = 66/184 (35.87%), Postives = 96/184 (52.17%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT Y+I+KKDK+G W       +K      D+  A VP L EG+ Y+FRV A NKAG   PS  T   + + +NL P ID+       ++  ++  +  KV GEPPP   W  NG ++  S+   +   +Y       L V  +   ++G YT+ AEN NG D+  + + VL
Sbjct: 3830 KWTPPENDGGSPITGYVIEKKDKYGEW-------EKTLEVPADETFATVPDLIEGQPYEFRVRAVNKAGPGEPSDATPTIIAKPRNLAPRIDRTNLIEVKIKAGQNFNFDCKVTGEPPPTTKWMLNGKEVRPSERVKVKHVDYN----TSLNVRMATRAESGKYTITAENINGRDEAFVKVTVL 4002

HSP 8 Score: 114.005 bits (284), Expect = 5.862e-23
Identity = 69/184 (37.50%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W +P +DGG PIT YII+K+DK+        T +K      D    +V  L EG  Y+FRV A NKAG    S  +K H+ R KNL P ID+       VR      + + V GEPPP   W    N ++ SD   I  ++Y       ++V  ++  D+G YTLVA+N NG D   L + VL
Sbjct: 4126 QWSRPEEDGGSPITGYIIEKRDKYSP------TWEKCAEIDGDTNRGRVDDLVEGTPYEFRVRAVNKAGPGEASEASKSHIARPKNLPPKIDRKYMLDVKVRAGSFFDFDVPVTGEPPPSKEWSLKDNVVMASDRIKIINEDYN----TKVRVMEAKRSDSGIYTLVAKNVNGKDSATLTVNVL 4299

HSP 9 Score: 102.834 bits (255), Expect = 2.544e-19
Identity = 64/184 (34.78%), Postives = 94/184 (51.09%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP +DGG PIT YII+ K+KFG  W      +     ATID       GL EG  Y+FR+ A NKAG   PS  TKP + + + ++P I      +  V+  +   + IK  GEP P+  W+    ++I      +  D+Y+     +L V ++   D+G Y LV  N +G+ +   D+VVL
Sbjct: 1166 WTKPENDGGAPITGYIIEYKEKFGKEWVTGKEIEGDVTQATID-------GLKEGTQYEFRIRAVNKAGPGEPSDVTKPIIAKCRFVKPYIVGDGLTNLVVKKGQVIKYDIKYAGEPEPEVHWFLGQKEIIQDSAERVTIDKYE--RNTVLTVRKTTRPDSGKYKLVLTNSSGTCESVADVVVL 1340

HSP 10 Score: 100.908 bits (250), Expect = 1.159e-18
Identity = 63/189 (33.33%), Postives = 97/189 (51.32%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFG-GWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVF--RSQMHDAGTYTLVAENRNGSDKIDLDLVVLAQ 430
            RW  PL DGG PIT Y+I+K++K    W        KAC  T  D + +V  L EG  Y+FRV A N AG   PS  +KP   + + L P ID+      +VR + +  + +K+ GEPPP  +W  N   +  +    I    Y        + F  + +  D+GTY + A N++GSD  ++++ V+++
Sbjct: 3241 RWTPPLKDGGSPITGYVIEKREKGAPKWI-------KACE-TGPDCKGRVDNLDEGVEYEFRVKAINAAGLGEPSDASKPITAKSRRLPPKIDRRNLRDITVRENEAFHFDVKIIGEPPPDVTWVINNKSIQQTTHRRIQNVPYNS------KFFNDKPERKDSGTYKITAVNQHGSDTAEVEVTVVSK 3415

HSP 11 Score: 96.6709 bits (239), Expect = 3.036e-17
Identity = 53/173 (30.64%), Postives = 83/173 (47.98%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGS 418
            W  P  DGG PIT YII+K+ +FG W       +KA     +    +VP L+EG+ Y+FRV+A NK G   PS  + P + + + + P  D        VR  +   ++I +   P P+ +W ++G +++         + Y        ++  S   D G YTL  EN +GS
Sbjct: 4422 WTPPKKDGGSPITSYIIEKRPRFGQW-------EKAAEIPGNQTSGRVPDLTEGQEYEFRVIAVNKGGPGEPSEASAPIVAKPRFIAPSFDTHLLHDLVVRAGQKISYNIPIVASPKPKATWNRDGVQIVADARH----EMYTTNTETSFEIPFSVRSDTGRYTLTLENEHGS 4583

HSP 12 Score: 94.3597 bits (233), Expect = 1.666e-16
Identity = 64/183 (34.97%), Postives = 90/183 (49.18%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVV 427
            W++P+ DGG PIT YII+KKDK+   W  AL T            +A V GL EG  YQFRV+A NKAG S P   +K    + + L P ID+      ++       +   V GEPPP   W + G   ++SD  V   +       ++  V R    D+G YT+ A N +G D + + + V
Sbjct: 3536 WKEPVSDGGSPITGYIIEKKDKYSTMWEKALETGTPVT-------KALVHGLIEGNDYQFRVIAVNKAGQSEPGEASKTFTAKPRFLAPKIDRRNLRDVTLSAGSMLKFDANVIGEPPPHIEW-RYGAIPLHSDRKVQIDNIDYNTKFSIRPVSRD---DSGDYTVTASNSSGKDSVTVQVTV 3707

HSP 13 Score: 92.4337 bits (228), Expect = 6.156e-16
Identity = 60/187 (32.09%), Postives = 95/187 (50.80%), Query Frame = 0 
Query:  245 RWEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVK--GEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            +W  P +DGG PIT YI+QKK+K    W +A+       + T       VP L+EG+ Y+FRV+A N AG S PS  +     + + L P I       + VR+    I+H+ +   GEP P+ SW    N+L ++D   I +  Y      ++ +  ++  D+G Y L+ +N +G D+    + VL
Sbjct: 4715 QWPVPKNDGGSPITGYIVQKKEKGSPYWVNAVHVPAGQNSTT-------VPDLTEGQDYEFRVIAVNAAGQSEPSEPSDLVTAKPRFLAPKIKTPL---QDVRIKAGLIFHVDIDFIGEPTPEASWSVGSNELTSNDRTTITSIGYH----TIVHIVNAKRSDSGLYHLLLKNSSGIDEGSFQVTVL 4887

HSP 14 Score: 75.485 bits (184), Expect = 1.407e-10
Identity = 58/184 (31.52%), Postives = 76/184 (41.30%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGG-WFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W  P  DGG  I  YIIQKK K    W D          A I      VP L EG+ Y FRV A N  G S PS  + P +   +  +P +D G     +VR       H+   G P P  +W+ N    +  DE      +      A L V  ++  D G Y L   N +G D   +++ VL
Sbjct: 5411 WRSPRSDGGSKIRGYIIQKKAKGDDDWSDV-------NGAPIPSNVYTVPNLKEGEEYLFRVFAVNDVGSSDPSRPSNPIVIEEQPNKPVMDLGGVRDITVRAGEDFSIHVPYIGFPKPTATWFAND---VIKDETDPRVHQQLADDFASLVVKNAKRSDGGQYRLQLRNSSGFDTATVNVKVL 5584

HSP 15 Score: 67.3958 bits (163), Expect = 5.999e-8
Identity = 33/88 (37.50%), Postives = 47/88 (53.41%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRP 333
            W  P++ GG+PIT Y ++K+++ G W           T T+ DL        EG  Y+FRV A N+AG   PS  T+P +  H+ LRP
Sbjct: 5311 WNPPINTGGKPITGYYVEKRERGGEWLKVNNYPTPNTTFTVQDLH-------EGSRYEFRVSAVNEAGPGKPSKPTEPIIAGHQRLRP 5391

HSP 16 Score: 65.0846 bits (157), Expect = 2.971e-7
Identity = 54/234 (23.08%), Postives = 102/234 (43.59%), Query Frame = 0 
Query:  211 RLELNGLLGQLGEELLAEEHVLSRLQLLLPMHLGR--------------WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDDKACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKGAAGSKSVRVHRSAIWHIKV--KGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            R+    L G+  E L  +  V+++ Q ++P   G+              W  P+ +GG  I  Y ++++D+  G +     +        DD       ++EG  Y++RV A N AG    S  +     +    +P +   A   + ++V      ++ +   G P P+  W K+G  L+   E +  + E +   T +L + +S   D G YT+ A N +G D  D++++V+
Sbjct: 4966 RVSAENLQGR-SEPLTTDRSVVAKNQFVVPGQPGKPECVDADKDHIKIKWTPPISNGGSKIIGYDVERRDRATGRWIKQNKEPVRYPEYYDD------HVTEGHQYEYRVSAINAAGAGKASETSSVFTAKPMKEKPKLYLDALIGRKIKVRAGEPINVNIPLSGAPTPKIEWTKDGRSLM---ETLRLSTETKSDRTQLL-IEKSVRDDGGMYTVTATNEHGKDSADIEVIVV 5188

HSP 17 Score: 63.5438 bits (153), Expect = 7.486e-7
Identity = 52/187 (27.81%), Postives = 80/187 (42.78%), Query Frame = 0 
Query:  246 WEKPLDDGGRPITHYIIQKKDKFGGWFDALVTDD---KACTATIDDLEAKVPGLSEGKWYQFRVVAANKAGDSPPSSETKPHLCRHKNLRPCIDKG-AAGSKSVRVHRSAIWHIKVKGEPPPQFSWYKNGNKLINSDEFVIATDEYQGGATAMLQVFRSQMHDAGTYTLVAENRNGSDKIDLDLVVL 428
            W KP  DGG PI  Y+I+K+         L+ +D   KA  A + D   KV GL E   Y+FRV A N AG  P SS +           P I    +    +V         +     P P+ +W  NG +++  +     T +    A+  +   +++  D GTYT+   N  G+D     ++V+
Sbjct: 5707 WTKPRHDGGSPILGYVIEKR---------LLNEDKWVKATPALVHDTTYKVTGLIENHDYEFRVAAENAAGRGPWSSNSDVIRASAAPFPPKITSDLSIRDMTVIAGEPFTITVPYTANPKPRPNWSINGEEVLTGERIKFETTDV---ASQFINK-KAKRSDTGTYTIYLTNTVGTDSASCKVLVV 5880
The following BLAST results are available for this feature:
BLAST of low quality protein: twitchin-like vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides)
Total hits: 5
Match NameE-valueIdentityDescription
EMLSAG000000001412.256e-10768.00supercontig:LSalAtl2s:LSalAtl2s1021:93859:109614:1... [more]
EMLSAG000000025714.157e-3851.70supercontig:LSalAtl2s:LSalAtl2s1518:43667:45350:1 ... [more]
EMLSAG000000010652.673e-3459.81supercontig:LSalAtl2s:LSalAtl2s1177:93008:93841:-1... [more]
EMLSAG000000087213.758e-2835.14supercontig:LSalAtl2s:LSalAtl2s541:438808:485939:-... [more]
EMLSAG000000066902.464e-1835.71supercontig:LSalAtl2s:LSalAtl2s371:395392:401754:-... [more]
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BLAST of low quality protein: twitchin-like vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt)
Total hits: 8
Match NameE-valueIdentityDescription
gi|74966877|sp|Q23551.3|UNC22_CAEEL1.410e-2435.14RecName: Full=Twitchin; AltName: Full=Uncoordinate... [more]
gi|160358754|sp|A2ASS6.1|TITIN_MOUSE4.002e-1530.34RecName: Full=Titin; AltName: Full=Connectin[more]
gi|384872704|sp|Q8WZ42.4|TITIN_HUMAN5.735e-1529.78RecName: Full=Titin; AltName: Full=Connectin; AltN... [more]
gi|296439237|sp|Q14324.2|MYPC2_HUMAN1.431e-932.14RecName: Full=Myosin-binding protein C, fast-type;... [more]
gi|81910387|sp|Q5XKE0.1|MYPC2_MOUSE7.171e-930.61RecName: Full=Myosin-binding protein C, fast-type;... [more]
gi|1076761195|sp|D3YXG0.1|HMCN1_MOUSE8.123e-932.06RecName: Full=Hemicentin-1; AltName: Full=Fibulin-... [more]
gi|85542049|sp|Q96RW7.2|HMCN1_HUMAN2.227e-831.30RecName: Full=Hemicentin-1; AltName: Full=Fibulin-... [more]
gi|6166595|sp|O70468.1|MYPC3_MOUSE8.616e-728.79RecName: Full=Myosin-binding protein C, cardiac-ty... [more]
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BLAST of low quality protein: twitchin-like vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR)
Total hits: 25
Match NameE-valueIdentityDescription
gi|1325263294|ref|XP_023333663.1|1.623e-8563.64twitchin-like isoform X4 [Eurytemora affinis][more]
gi|1325263307|ref|XP_023333672.1|1.800e-8563.64twitchin-like isoform X5 [Eurytemora affinis][more]
gi|1325307310|ref|XP_023335272.1|1.257e-7657.89twitchin-like [Eurytemora affinis][more]
gi|1068388476|ref|XP_018043887.1|2.849e-3543.16PREDICTED: twitchin isoform X21 [Atta colombica][more]
gi|1068388472|ref|XP_018043886.1|2.849e-3543.16PREDICTED: twitchin isoform X20 [Atta colombica][more]
gi|1068388465|ref|XP_018043883.1|2.853e-3543.16PREDICTED: twitchin isoform X18 [Atta colombica][more]
gi|1068388462|ref|XP_018043882.1|2.854e-3543.16PREDICTED: twitchin isoform X17 [Atta colombica][more]
gi|1009363002|gb|KYM88060.1|2.875e-3543.16Titin [Atta colombica][more]
gi|1068388479|ref|XP_018043888.1|3.013e-3543.16PREDICTED: twitchin isoform X22 [Atta colombica][more]
gi|1068388468|ref|XP_018043884.1|3.218e-3543.16PREDICTED: twitchin isoform X19 [Atta colombica][more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
scaffold175_size286436supercontigscaffold175_size286436:176561..181441 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
maker2018-02-12 .496401
T. kinsejongensis vs L. Salmonis peptides2018-04-19
T. kingejongensis peptided Blastp vs. SwissProt2018-04-19
T. kingsejongensis proteins Blastp vs. NR2018-05-15
Properties
Property NameValue
Note-
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
maker-scaffold175_size286436-snap-gene-1.53-mRNA-1maker-scaffold175_size286436-snap-gene-1.53-mRNA-1Tigriopus kingsejongensismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at scaffold175_size286436:176561..181441-

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>maker-scaffold175_size286436-snap-gene-1.53 ID=maker-scaffold175_size286436-snap-gene-1.53|Name=low quality protein: twitchin-like|organism=Tigriopus kingsejongensis|type=gene|length=4881bp|location=Sequence derived from alignment at scaffold175_size286436:176561..181441- (Tigriopus kingsejongensis)
ATGCTGCGAGATCTGACAGAGGATAAAGTCGCGGAACTCTTGGGGCTCGT
GGCTGAAGTGAAGCACGCGTCCGTCCCGACACACGACAGTGAGTTGTCGC
TCACCCACATGGATCACGAGCTGAGTGCGGAACATGGAGTCGTAGCCCGA
GGTCACGATGCTCACCTTGACCGAGGTGCGGGTGGGCGAGCTCAGGGTGC
CCAAGTGGCACGAGATGAGCGGGTCCTTGAGCTCCCGGGCCATGGTATCC
AGGACGAACATGGTCCTCAGTCGGAGCACCACATGCTGGGCCTGCTGAAT
GATCTGTTCCAACAGTGTGGGCTGGGCGGCCATCTCGAGCAAGGCCTGGC
GATCCGCGGCCCGCGGTCCAGCCAATCGGCGGCGCTTGGAGATGCCCAAG
GGGGCCGAGCTGAGGGCGGGCGCGTCCGGGTTGATATTGCGACTGTGCCG
GAGGCGAAGGAGCTGGTGGAGGGAGTGCTCGAGCACGTGGGAGTGGACCA
ACTGGGACTGCATGGCCCGGGCCGGCCCCTTCTTCTCGGCCCCGGTCGAG
TGGCAGAGCGAGATGATGAGCTGGATGTCGGTGAAGAGGCTGGCCGAGAT
CTGATGGCCCACCACCATGTGCGGAATGGGCGCCTGGAGCTGAATGGCCT
CCTTGGCCAGCTGGGAGAAGAGCTCCTGGCAGAAGAGCACGTTCTGAGCC
GCCTCCAGCTTCTTCTGCCAATGCATCTCGGGCGGGTTGGCGCCGAAGGC
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
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NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCAGAACCACC
CTCGGCTCCAAGGGATCCGGATATCATTGATTTCGACAACAAATCTGTCA
CGTTGAAGTGGGAGAAACCTCTAGATGACGGAGGAAGACCCATCACCCAC
TACATCATCCAGAAGAAGGACAAGTTTGGTGGCTGGTTTGATGCCCTTGT
GACTGACGATAAGGCATGCACGGCCACCATTGACGACCTCGAGGCCAAAG
TTCCAGGCCTTAGCGAGGGCAAGTGGTACCAATTCAGAGTGGTGGCAGCC
AACAAAGCCGGAGATTCTCCTCCCAGCAGCGAGACCAAACCTCACCTCTG
CCGGCACAAGAACCGTAAGATTCCTTCGGAGCTTATTTTCTTCAGTATAT
TTTGTTAGCGAGTCCAGAGCTGAGCCATTGCTCCTCGCCATGAAGCCATA
GCTACTGCCAAATGAGCGGTTCCAGAGTCAGATCAGGAACATTAACATAA
ATTGTGCTTTGCAGTTCGCCCGTGCATTGACAAGGGAGCCGCAGGTTCCA
AATCCGTGCGAGTCCACCGTTCAGCCATCTGGCACATCAAGGTCAAAGGT
GAGCCGCCACCCCAGTTCTCGTGGTACAAGAACGGGAACAAACTGATCAA
TTCGGACGAGTTCGTCATCGCCACGGACGAGTATCAGGGGGGAGCCACGG
CCATGTTGCAAGTCTTCCGGTCCCAGGTGAGGTTGAACCACGGATATACA
TACTAAAGTCCCTATATGAATACTTTGTCTTTAATACGTATACTAATCCA
GACGGATTATTCGGCAAAAGTTTCAGGCACGCTCGCAACCTTTCAAGCTT
TTGTGGTCGCGAGGATGTAAACCATCCGGGTCTATGTACTGCTATGGAGT
GGCCTTTCAGGGGGTGGCCAGCGATCCCTGATCAGGCCATTGGGCGGGAA
ATGAGCCCATCGAGCACTTATCCGTGGTGGCTTAGAGTTTCCCTAGAACC
AAACCTGCGAAAGATGCACCTTTATCAAATTGGAAGGCTATACTTATCAG
AACATAGCTTATGGACTGAAAAGCCAGGCCTCCAGAATCGAAAGCGGGCG
ATTTGGAAATATTCCTTCCTTCTATGACACTGAGTGGGTCTCAAAAACTG
TCCAATCAGCGGCCATCCAAAGCCAATGGGTGATAAGATTGACACTCACG
ACCCTGGTTAAGTTAGCTATATAGCAGTACATAAACTCAGTCTCGTTGGC
ATCCCGGCGATCGTGGTCCAGTGCGACCATGGTTGTTCTTTAGGGGTAGG
AGTTTGCCCGATTAGTATACGTATACGTACATACGGTAAAGGGCTGGGCT
GGAGAGCAACCAGTTCGTAGAGGGTGACGGCATTCTGACGGGTTCGTTTC
TCCGCAGATGCACGACGCTGGCACTTACACTTTGGTGGCCGAGAATCGGA
ATGGCTCGGATAAGATCGACCTGGACCTCGTTGTGTTGGCCCAAATGGAC
GATGGGGACTGCAACATGTTCAAACATGGAATGCGGGGATGCACGTGCTC
AGCCGGTTTCAGGTTTGGTCCCGTGTGATTTGACAGAGGGCGCTGTCGCC
AATGGGCTTCTTCCCTTTCCTTTGCAGACATAACGAGTTGAGCGCCCAGC
AAATTCTGACGGTGGACTTTGGCGACCCTCCGCCCAGTCTCTGAGGCCTG
GATGTCTTCCAACCTTTCAAAACTGAACTCATGTTGTCTTCCAAATACCA
CTTGGTCCCATGAGAAATGCTCTTTGTTATTCTGTTGTGTCGCTCTGATT
CCCTTAGTTTGTAAGCCTTTCTACGTTGTCCCGTGCTTTTGAGTATGCAA
ACTGTTCTCATCATGGCTTTAGACAATGCTCGCATTGATTTCATTGTCGC
CAATAAAACGCTTGTGGATTTTCAAGATTTA
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Synonyms
The feature 'low quality protein: twitchin-like' has the following synonyms
Synonym
Tk09301
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