BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000000839 (supercontig:LSalAtl2s:LSalAtl2s1141:106424:108541:-1 gene:EMLSAG00000000839 transcript:EMLSAT00000000839 description:"maker-LSalAtl2s1141-augustus-gene-0.8")

HSP 1 Score: 344.739 bits (883), Expect = 1.829e-110
Identity = 221/648 (34.10%), Postives = 338/648 (52.16%), Query Frame = 0

Query:   45 VKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR-------------------------------------LHHDNITWTSRVTLTQYEW-EKED-ILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVK-PSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYS 652
            VK+R     P N  PI +P +++E+    G   AL  +  +G   +WTF+DY  DVR+ A A +  GL+   GV I+  NSPEW I+ + +I+AGG+  GIY T++  + +Y+A++   +I+ +ENQ   + L + + ++  LP ++KI+    +   P     V+SW   + LG                                      L HDNIT+T++ T   Y+  EKE+  ++YLPLSH+AA++VD ++      TVYFADK ALKG+L + LRE  PT F  VPRV+EK+Q K+  A+    G+KK I +WAKK G RY       P +   + Y +A+K+V  +V   LG D+ V      S  A L I+T EYF SL + + +L G +E  GP   N      KPG VG+   G    I D D    GEI  +                              G LD  G L + GR KELI+ +GG+NI P+ IE ++K +LP ++SN +V+G+ + +L+C +T +V  D  +++P++ L      W   N +   + T  +++E       +   IQ G+   N +A+S  +K+QK+ ++P++FSV   ELGPTLK+KR  I +KY  ++  MYS
Sbjct:   22 VKLRTGSEVPSNYDPISIPQLLEETASEAGYIPALRVR-RNGDLITWTFRDYLKDVRTAAKAMLKCGLEEGNGVCILGFNSPEWFIADLGAIYAGGIPSGIYPTNNPDSCKYIASHCHCTIMFVENQ---EQLQKIQSIKTQLPHLRKIIQYIGEPTDPD----VLSWNAFISLGTEPELEXLLEISLSKIXANKCCHLVYTSGTTGNPKGVMLSHDNITFTAKNTGEHYKLKEKEERFVSYLPLSHIAAVIVDIFIPITFKVTVYFADKNALKGTLTETLRECHPTLFFGVPRVYEKMQEKIMGASKSTKGLKKHIGRWAKKTGYRYNKSLITHPNQDKDIQYKVANKVVFQKVKALLGFDKCVH---FVSSGAPLSIETLEYFMSLDILIMELYGMSECCGPHXANTLR-AWKPGKVGRDLPGFLTKI-DKD----GEIHMK------------------------------GELDSSGFLRVTGRRKELIITAGGENIPPVLIEDKLKASLP-ILSNAMVIGDNKKYLSCFLTLKVNEDSETHEPSDRLSPLSEEWC--NKLGSHVRTVSEILE--NPEEQVLAAIQEGINKYNINATSNAQKIQKWIIIPKDFSVSTGELGPTLKMKRYFILKKYEALVKKMYS 617          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000003336 (supercontig:LSalAtl2s:LSalAtl2s182:521806:564306:1 gene:EMLSAG00000003336 transcript:EMLSAT00000003336 description:"maker-LSalAtl2s182-augustus-gene-5.28")

HSP 1 Score: 306.605 bits (784), Expect = 1.200e-90
Identity = 209/619 (33.76%), Postives = 317/619 (51.21%), Query Frame = 0

Query:   62 VPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSRVTLTQYEW--EKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTET---TGPQTTNLAGPKCK---PGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHH----LRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTL 632
            VP M+K      G + AL+  + +G+W  W +  YY D++  A AF+ LGL     V I + N PEW IS++ +I+AGGLS G+Y T+S  T +Y+ NN+   ILV+EN   L  +     + K + T+KKIV++E D  +P +   ++ WK L+ELGR                                    L HD IT+ ++  + +++W   K  +++YLPLSH+AA  +D Y++      VYFADK ALKGSL D L+EVRPT F  VPRVWEK Q  +++ +      +  ++Q  K     Y      G  K  + + +  KL+  +V QALGLDR +      SG A L   T ++F SL + +Q+  G +E       Q ++     C    P T  K        ++  D +  GEI  + R V MGY+  E  T + F  EG+F +               GR+K+L+L SGG+N+AP PIE  IK  LP ++SN VVVG+   F++  +T +V+ +    +PT+ L +SV    K  N+    TT  +++      +H    + + IQ G++ AN  A S   KV+K+ +LP+E S+   E+G TL
Sbjct:   47 VPNMVKNHCLAGGSKTALAI-MRNGNWVKWNYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTI---SHILKNVTTLKKIVVIEEDGFRPGEN--LILWKDLLELGRKEGIDRLNDRLSHISVNQCCSLIYTSGTTGNPKGVMLSHDIITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHY---YENGDQK--VIHKIGDKLIFQKVKQALGLDRCLE---FISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFKTGTCGTIIPSTHAK-------VVKSSDDDIGGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHT---------------GRLKDLLLTSGGENVAPAPIENAIKHYLP-VVSNAVVVGDAEKFISVXLTIKVKSNE-DGEPTSELTDSVKKDFK--NLGCNYTTVGEIL------YHKPPGVMKAIQKGIDEANKRAISNAAKVKKWCILPRELSLEAGEIGITL 619          

HSP 2 Score: 229.18 bits (583), Expect = 4.497e-64
Identity = 178/637 (27.94%), Postives = 300/637 (47.10%), Query Frame = 0

Query:   56 NLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVK---KIVLVETDIIQPHQKSFVMSWKQLVELG----------RLH--------------------------HDNITWTSRVTLTQYEWEKE-DILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYS 652
            +L P+ VP+ +K +  + G + A++ K  +G W  WT+  YY ++R  A AF+ LGL+    V I + N PEWMIS++ +I+AGG + G+Y ++S    +Y+ NN+   ILV+EN   L      ++++ +L  +K   K+V++E D     + S +  W+ L+ELG          RLH                          HD +T+T++  + ++ W K+  I++YLPLSH+AA  +D Y+       +YFADK ALKGSL   L+EVRPT F  VPRV+EK Q  +++  S+    +K I+   K     Y        +K      L ++++  ++  +LGLD+         G A L   T ++F SL + ++   G +E      ++ A  + K GT G  +         P + G      R + + +    E +  I                                ++    KN  P+             +SNVV +G+ + F++  +T +V  D  + QP+  L  SV  W   +++    +T  ++I        +   IQ G++MAN    S   +V+K+ ++P+E S+   E+GPTLKLKR     +++K+  N+YS
Sbjct:  659 DLQPMSVPSFVKHNCLKGGSQIAMAIK-RNGDWMRWTYSQYYREIRLAAKAFIKLGLKPFHTVSIYSFNCPEWMISNLGAIFAGGFATGLYPSNSASMNEYLMNNSRCQILVVENNXVL------QKVQPILENIKHLLKVVVIEEDGFIADKLSIL--WRDLIELGKTEEEDQLKQRLHNIAANQCCSIIYTSGTTGNPKGVMISHDMLTYTAKTVVEKHGWTKKISIVSYLPLSHIAANALDIYVVICCRGELYFADKSALKGSLVRTLKEVRPTVFFGVPRVYEKFQEGIEEKISKLKKSQKFITNILKDAAFCYNTTGNKKFIK-----NLGNQMLYKKIKDSLGLDQC---QEFSCGGAPLMPTTTDFFLSLDIVIKVAYGLSECG--IFSDFAPSEYKKGTCGSIF---------PSTQG------RKKELLITAGGENIAPI--------------------------------LIEDNIKNFLPV-------------LSNVVAIGDLKKFISVFLTFKVNSD-TNGQPSTELSNSVKEWC--HSLGYEASTVTNIIR--DQPPEVMSAIQKGIDMANEIXVSNAARVKKWSLIPRELSMDNGEMGPTLKLKR----NEFSKIHFNIYS 1207          

HSP 3 Score: 75.0998 bits (183), Expect = 3.625e-14
Identity = 51/151 (33.77%), Postives = 85/151 (56.29%), Query Frame = 0

Query:  487 LIICGR---IKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQI--IQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTL 632
            +++C R    K+L + +G +NIAPI IE +IK+ LP ++SNVV +G+ + F++  +T +V  D  + QP+  L   V  W        +++     +  I  +  L  I  IQ G++ AN  A S   KV+K+ ++P+E S+   E+GPTL
Sbjct: 1304 VVMCCRGRKEKQLFITAGVENIAPILIEDKIKNFLP-VLSNVVAIGDLKKFISVFLTFKVNSDK-NGQPSTELSNLVKDWC------HSLSYEASTVTNIIRDKPLEVISAIQKGIDKANKIAVSNAAKVKKWSLIPRELSMDNGEMGPTL 1446          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000006219 (supercontig:LSalAtl2s:LSalAtl2s33:201552:203709:-1 gene:EMLSAG00000006219 transcript:EMLSAT00000006219 description:"maker-LSalAtl2s33-augustus-gene-2.8")

HSP 1 Score: 273.478 bits (698), Expect = 4.540e-85
Identity = 164/467 (35.12%), Postives = 251/467 (53.75%), Query Frame = 0

Query:   33 ATDFISWNKHVPVKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSF-----VMSWKQLVELGR--------------------------------------LHHDNITWTSRVTLTQYEWEKEDI-LNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMG 455
            A  + + + +  VK+R   + P ++ P+ VPT++K + + +    A++ K +D  W  WT+++YY + +  A  F+ LGL+    VGI+  N+PEW +S V +I+AGG   GIY T+S + V+YVAN+  V +LV+E+          ++L+KV    +++  ++  I+  +Q+ F     + SW++ ++L R                                      + HDN+TWT+R +      +  D  ++YLPLSH AA M+D +M    G ++YF D+ ALKGSL   LREVRP  F  VPRV+EKI+ KM++    N+G KK+  +WAKK G  + ++   G     + Y LA KL+  +V + LGLD+A    G+  GAA +   T EYF SL + L D  G +E++GPQT N  G   +  TVG    G    I +PD NG GEI   SRNV MG
Sbjct:   10 ANSYTNSDPNGAVKLR---SSPDSILPLSVPTLLKNASDSYPKSVAMAVKRAD-HWNKWTYKEYYEEAQLAAEGFIELGLEPFHSVGILGFNAPEWSLSLVGAIFAGGFGAGIYATNSAEAVKYVANDCRVQVLVVEDS---------KQLQKVNAIKEQLTFLKAIIVYGYQEKFEGGDNIYSWREFMDLARNAQKKGENVQNRLENISINQCCSLIYTSGTTGPPKGVMMSHDNVTWTARTSNHYLGLKHGDRGVSYLPLSHSAAQMMDIWMPIAQGMSMYFCDRNALKGSLVSTLREVRPAYFFGVPRVYEKIEEKMRELGKANTGFKKAFGEWAKKTGLNHNMKSLEGQQPGGISYPLAKKLIFKKVKEGLGLDKA-RMCGI--GAAPVSRKTLEYFLSLDIPLYDCYGMSESSGPQTGNRPGNH-RFMTVGGNVGGCVTKIYEPDENGEGEICMSSRNVMMG 459          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000010718 (supercontig:LSalAtl2s:LSalAtl2s70:458722:474012:1 gene:EMLSAG00000010718 transcript:EMLSAT00000010718 description:"augustus_masked-LSalAtl2s70-processed-gene-4.2")

HSP 1 Score: 150.984 bits (380), Expect = 2.877e-39
Identity = 110/466 (23.61%), Postives = 212/466 (45.49%), Query Frame = 0

Query:   33 ATDFISWNKHVPVKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETD-------IIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSRVTL--------TQYEWEKEDIL-NYLPLSHVAALMVDCYMSWLGGNTVYFADK---LALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAK--------KEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQ 435
            A +F S  ++  VK+R  ++  G+  PI VPT+++   E + D  A   K  D  W SWT++ Y  +V +VA +F+  GL     V I+ +NSPEW++S VA+I++GG++ G+    S   +  +  +    ++V++N+ FL  +L    ++  LP ++ I+ ++ D        +    K  ++SW++++ LG                                     + HDN+TW ++ ++         +     E+IL + LPLS ++A ++D Y +     T+  +     L    +  D + +V+PT   A   ++E+I  K+++     SG++K +  W+          E ++    R+L   + +    LA   V  +  + LG     ++   +S  A +  +   +     + + +  G TE  G  T N+    CK G+ G+P  GV+ +I+
Sbjct:   47 AEEFSSTRRNGCVKLRKVEHGLGSEEPISVPTLLQNIAENYPDVVAFKIK-RDEEWISWTYEQYLKEVNTVAKSFIXXGLHRHXSVAILGNNSPEWIVSDVAAIFSGGIAVGLNKWLSPDEIAKICIDCKADVIVVQNEAFLKKILL---IQHKLPELRTIIQIDGDPPLSDKRRLHRTHKKIILSWEEMIVLGHNLTDDRLRDRLKKVAINQVCTIIYTSGANETPKGVMISHDNLTWCAKTSMGLIRAPGFNRMPAPGEEILISVLPLSSISAQIIDIYYTLCVVGTLCISSNKNLLTQGDAFFDTVFDVQPTVLFAPTLIYERIFHKLRNLKRSMSGIQKIVLDWSSCTLRDKHLNEQKKIKATRKLNQWQTA----LAKNTVTKKYKEMLGF---TSRTVFFSRGAPMAREVLRFLSGYDIVVHETFGQTENCGLLTANIPKRYCKIGSTGRPVPGVKMNIE 501          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000010073 (supercontig:LSalAtl2s:LSalAtl2s663:674899:681644:1 gene:EMLSAG00000010073 transcript:EMLSAT00000010073 description:"maker-LSalAtl2s663-augustus-gene-6.10")

HSP 1 Score: 100.138 bits (248), Expect = 4.033e-22
Identity = 151/670 (22.54%), Postives = 263/670 (39.25%), Query Frame = 0

Query:   67 KESVERFGDRKALSTK---------LSDGS-WKSWTFQDY----YHDVRSVALAF----VALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSF-----VMSWKQLVELGRLHHDN-----------ITWTSRVT----------------------LTQYEWEKEDILNYLPLSHVAALMVDCYM-------SWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQ-SLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGV---------GEISTRSRNVFMGYINEELQTIQTFTPEG----WFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGE-GRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGP-------TLKLKRPVIHEKYAKVIDNMY 651
            K +V   GD+  L T+           DG  +K W   +Y    ++++  ++  F      LGL++KE + I A +  EWM S++           +Y    +  + +  N T VS L+    T  D L + + + +  P++K I+  E  +    +  +     ++S+++++ELG                 I +TS  T                      + + +  +E  L YLPL+H+  L+ +  M        +   NT+       +KGS  D    +RPT   AVP V ++I   + +  S+ S   +S+  +       Y   RRLG   P     + + L+   +   +G    V    + SG A L  +  +Y   +L + +    G TE+    +  +   +   G VG P  GV   + + +  G          GEI     N+  GY     +T + F  +     WFK+GD+G   E+G L I  R K+L+    G+ ++   +ET IK     L+ NV   G+  + +   L+ P        N+ T  L ES+ ++   N   E +  +  +I  +Q                   +  R   ++KF V P   ++  E   P         KL+R  I + Y K +  MY
Sbjct:  101 KLAVSLHGDKDCLGTRHVLEEKDEVQPDGKVFKKWAMGEYQWKSFNEIEELSTDFGKGLRELGLETKENICIFAESKAEWMXSALGCFKQTFPLVTLYANLGEDAIAHGVNQTQVSHLI----TTHDLLPKLKNVLERTPSIKYIIFFEDQLNATDRSGYKXGVRILSFQEVIELGSKSETKATPPTKDDPAIIMYTSGSTGVPKGVILPHESLIGTIAAFHLVVKDKIPEETYLGYLPLAHIYELLAEMTMLIHGIKVGYSSPNTMIDKSTKVMKGSKGD-CSVLRPTLMCAVPLVIDRIYKGIHENLSKKSKFMRSLLHFCYHYKSHY---RRLGMRTP-----IMNALIFRPMRAIVGGKVRV----IVSGGAPLSHNIHDYVSCALDIPIHQGYGLTESCAGGSV-MHREELSTGRVGPPGQGVLIRLINWEDGGYRVTDKPRPRGEIVIGGPNIAHGYYKLPQKTAEDFYKDENNVRWFKTGDVGEFAEDGTLKIIDRKKDLVKLQYGEYVSLGKVETVIKTC--PLVENVCAYGDPTKSYCVALVVPD------RNKLTE-LGESIGIY---NASLEDLCMNEKVIATMQK---------------ETASYGRKNGLEKFEV-PGAITLCTEMWTPESGLVTAAFKLRRKPIQDFYQKELKKMY 724          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000004934 (supercontig:LSalAtl2s:LSalAtl2s259:871050:904452:1 gene:EMLSAG00000004934 transcript:EMLSAT00000004934 description:"augustus_masked-LSalAtl2s259-processed-gene-9.9")

HSP 1 Score: 94.7449 bits (234), Expect = 2.167e-20
Identity = 149/653 (22.82%), Postives = 263/653 (40.28%), Query Frame = 0

Query:   81 TKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPT----VKKIVLVETDIIQ-----PHQKSFVMSWKQLVELG---RLHHDN-------------ITWTSR-------VTLTQYE------------------WEKEDILNYLPLSHVAALMVDCYM-------SWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQ-SLGMELQDLLGCTETTGPQTTNLAGPK--CKPGTVGKPYLGVRNSIQDPDSNGV---------GEISTRSRNVFMGYINEELQTIQTFTPEG---WFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGE-GRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLG---EELG---PTLKLKRPVIHEKYAKVIDNMYSEI 654
            TK   G ++  T++D     R+ +     L    K+ + + A +S +W++S+            IYT    + V +    T   +++        NLL  + L+K+L +    ++ I+ +E +        P Q   V S+ +++E G      +DN             I +TS        V L+ +                    +KE  + YLPL+H+  L V+  +        + G NT+  +  +  +G + D + E++PT    VP + ++I   +    S N    + +  +  +   ++   R+LG   P     + + L   ++ Q LG + +     + SG A L     E+ +    ++L    G TET G     + G K     G VG P+ G    + + D             GEI      + MGY     +T + F  E    WFK+GD+G+L   G L I  R K++I    G+ ++   +ET +      L+  + V GE  R+ +  LI P       + +   +L+ES+ +   RN             E++  N+ LR       ++  A A+    K+ KF +    + + G    E G    + KLKR  I  KY + I+ MY+EI
Sbjct:  204 TKYDQGEYEWLTYEDVNTISRNFSKGLRHLKFLPKDKILLYAESSAKWLVSAFGCFKNSMTVVTIYTNLGDEGVYHCIEQTDAKVIITS-----QNLL--KRLQKILASKGGLIQTIIYLEKEEFSLAQTTPEQDVNVYSFNEILEKGLKSYSQNDNEEDWRPNSEDIAVIMYTSGSSGVPKGVVLSHFNIMSNIFGIMSFLNQNFVRLDKESYIGYLPLAHIFELSVEIALFGKGIPIGFSGPNTLMNSGSMVKEGQIGD-ICELKPTYMAVVPLIADRIYKIIIQKVSENGPFSELLFNFCYEYKSKW---RKLGMNTP-----IINLLFFKKIQQFLGGNVST----IVSGGAPLSAQAHEFMEIVFDLKLSQGYGLTETCGGF---ILGRKEDFSLGEVGHPFPGPFVKLTNWDEGEYTVKDKPYPRGEIHVGGGLIAMGYFELMDETEENFYEENGVRWFKTGDIGQLQSSGALKIIDRKKDIIKLQNGEYVSLGKVETYL--GTHPLVDIICVYGEPERNCIVGLIIP-------NEKSIKILKESLGL---RN-------------ESVYDNNLLRS------QVRQALANHSEAKLLKFEIPKAVWLIHGPWTAENGLITTSYKLKRKAIQNKYQEKINIMYNEI 802          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000009272 (supercontig:LSalAtl2s:LSalAtl2s591:175091:177076:1 gene:EMLSAG00000009272 transcript:EMLSAT00000009272 description:"augustus_masked-LSalAtl2s591-processed-gene-1.0")

HSP 1 Score: 80.8777 bits (198), Expect = 3.714e-16
Identity = 98/435 (22.53%), Postives = 181/435 (41.61%), Query Frame = 0

Query:  240 EKEDILNYLPLSHVAALMVDCYM-------SWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQS-LGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGV---------GEISTRSRNVFMGYINEELQTIQTFTPEG---WFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYSEI 654
            E++  + YLPL+H+    ++  M        + G NT+  A  +  +G + D  R ++PT  +AVP + ++I   +Q   S+    K+++          +C Q R    K      + +KL+  +V ++LG +       +  G A L  +  ++ ++ L +++    G TE+ G   T         G VG P  G    + + +  G          GEI      V MGY N   +T ++F  +    WF +GD+G+L   G   I  R K+L+    G+ ++   +E   K  +   +  +   G    F    +    +   +++   N  EE     L R+               I S+  +  I    L++ +A    + E  ++  + P+ ++     +   LKLKR  I EKY + ID MY+++
Sbjct:  243 EEDRYIAYLPLAHIFEFTLEMTMILRGTSIGYSGPNTLTDAGXMIKRGHMGD-ARALKPTSMIAVPVILDRIFKGIQFKVSQEGAFKENLFT--------FCYQYRSMWRKLGFDTPIMNKLIFKKVRESLGGEI----QSIIGGGAPLSPEVHDFLETVLCVKISQGYGLTESCG-GITLCHYDDYHVGEVGYPLPGAYIKLVNWEEGGYKVNNSTAPRGEIHVGGNVVAMGYYNMSEKTKESFYEKDGIRWFPTGDIGQLQPTGAFKIIDRKKDLVKLQMGEYVSLGKVEGAWK--IHPFVDAICSYGNSFKFDIVAVVVPDKYKFLNHLKNNHAEED----LNRD------------FSTICSDPEIVIIFLKTLQVYSAKKLEKFEIPKRXLLEPEPWTPDCGLVTAALKLKRRTIQEKYQEDIDRMYTQL 645          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000007052 (supercontig:LSalAtl2s:LSalAtl2s3:1758018:1760492:-1 gene:EMLSAG00000007052 transcript:EMLSAT00000007052 description:"maker-LSalAtl2s3-snap-gene-17.37")

HSP 1 Score: 75.485 bits (184), Expect = 2.490e-14
Identity = 107/463 (23.11%), Postives = 189/463 (40.82%), Query Frame = 0

Query:  221 LHHDNITWTSRVTLTQYEWE---KEDILNYLPLSHVAALMVDCYMSWLG-------GNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQ-SLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKP--YLGVR-------NSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEG---WFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEG--RHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGP-------TLKLKRPVIHEKYAKVIDNMY 651
            L H N+  T +   + +E +    +  + +LPL+HV  L+ +  M   G        NT+     +   G+  D    ++PT    VP V  +I   +         +  SI         R+C   RL  ++  L   L +K++  ++H+ +G    +    + SG A L  +  ++ +  LG+++    G TETT   T  +       GTVG P  +L ++       N          GEI      V  GY     +T + F  E    WF++GD+G + E G + I  R K+L+    G+ I+   +E+ +K     ++ N+ + G+    + +A ++  +  +   ++Q   +  +  T     NN  E       +I+ +     LR++          H S +  K+Q+F   P    +  E  GP         K+KR  I E Y K +D MY
Sbjct:  324 LTHRNLVSTLKSLNSTFEIKISSNDKYIAFLPLAHVLELLCEMMMVIRGVRIGYSSPNTLLDTSTMIKSGTHGD-AAVLQPTVMAGVPLVLTRIYKGISQKMDSRGELFNSIF--------RFCYDYRLKSIRNGLPTPLLNKILFGKIHKLIGGKVKL----IISGGAPLSDEXHDFXRVCLGVDVLQGYGLTETTASATV-MDINDISTGTVGPPNQFLDIKLVNWEDGNYKVTEQPRPRGEILIGGDCVSNGYYKMPEKTKEDFLEERGKRWFRTGDIGEVTELGTIKIIDRKKDLVKLQYGEYISLGKVESALKTC--PVVDNICIYGDSFKSNCVALIVPNKTVLGKWADQKLGMYSQ--TEIEHNNNAFERCCKDERVIDMV-----LREV--------RLHCSKK--KLQRFET-PVAVYLCKENWGPETGLVTAAFKIKRRPIQEFYQKDLDQMY 752          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000011396 (supercontig:LSalAtl2s:LSalAtl2s787:62044:87799:1 gene:EMLSAG00000011396 transcript:EMLSAT00000011396 description:"maker-LSalAtl2s787-augustus-gene-0.76")

HSP 1 Score: 73.1738 bits (178), Expect = 1.650e-13
Identity = 111/530 (20.94%), Postives = 220/530 (41.51%), Query Frame = 0

Query:   66 MKESVERFGDRKALSTK---------LSDGS-WKSWTFQDY----YHDVR----SVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVE------TDIIQPHQKSF-VMSWKQLVE--------------------------LGR-----LHHDNITWTSRVTLTQYEWEKED-ILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLAL--------KGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQS-LGMELQDLLGCTETTGPQ--TTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGV---------GEISTRSRNVFMGYINEELQTIQTFTPEG---WFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIK 515
             ++ V+++G+R  L T+           DG  +K +   DY    Y+ +R     +A   + LG++ +  + I++ +  EW++S  A+         +YT      V+   N T V ++++ ++     L + + + +  P +K +V++E      +D+   +Q +F  + ++ L++                           GR     ++H +     R  ++  ++   D  L YLPL+H+  ++ +    + G   + ++  L L        KG   D     +PT   AVP + ++I   + D   R  G       WA +E   Y +  ++          L +K +   V   LG    +    L +G A +  +T  + ++ L + +    G TE +G    +       C+   VG P +GV+  + + +  G          GEI     ++  GY     +T   F  E    WFK+GD+G+++ +G L +  R K+L+  SGG+ I+   +E+ +K
Sbjct: 1904 FEDVVDKYGERDVLGTREIFSEENELQPDGKVFKKYLLGDYSYISYNGLRRQTSDLADGLMDLGIEPRSRIAILSESRQEWVVSMYAAFKNNYTLVTLYTNLGNDAVEEGLNETEVPLVIVSDEV----LPKFKGILEACPLIKYVVVIEAGNGKKSDLSVNYQNNFQFIMYRDLLKRRSKKKYNRSIPSTNDTAIIMYTSGSTGRPKGVMVNHGDAIAGLRSVISYTDFTTSDRYLAYLPLAHIMEIVAEMACLYCGVK-IGYSSPLTLTDNGSKVKKGCKGD-ANVFKPTLMCAVPLLLQRIYKGIIDNM-RKKG-------WAMEELFNYFVAYKIKWQXRGFDXPLLNKTLFRTVRYFLGGRVRL----LLNGGAPISHETHLFTRTTLSVPVLQAYGLTELSGAAAISNEYDFSTCR---VGPPLIGVKVKLVNWEEGGYTINDKPFPRGEIHCGGESLCQGYFKNPEKTADDFYEEDGIRWFKTGDIGQMEADGVLKLVDRKKDLVKLSGGEYISYGKVESILK 2412          

HSP 2 Score: 66.2402 bits (160), Expect = 2.210e-11
Identity = 102/489 (20.86%), Postives = 195/489 (39.88%), Query Frame = 0

Query:   86 GSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDI-----IQPHQKS---FVMSWKQLVELGR------------------------------LHHDNITWTSRVTLTQYEWEKED-ILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVR-------PTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRL-----GPVKPSLGYTLAHK---LVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGP--QTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEG---WFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIK 515
            G++K  T+ +   +  ++A  F +L L +K  + + A    EW++++ A+         +YT   +  V +  N T V ++V      +D L + +++ K  P +  ++++E +      ++P++ +   F+M ++ L+                                  + H NI    +  +    ++  D  + YLPL+HV  L+ +  +  + G  + ++  L L        R  +       PT   +VP V E+I   + D   R          WA +E   Y +  ++     G   P L  TL  K    +  RV   +     ++ D        L +   +     G  L +L G    + P  +TT   GP      +        N   +  S   GEI     ++ +GY     +T + F  E    WFK+GD+G+++ +G L +  R K+L+  SGG+ I+   +E+ +K
Sbjct: 1415 GAYKFMTYDELKEESYNLAEGFRSLKLPTKTHIAMFAETRKEWILTAYAAFINNFTLVTLYTNLGEDGVIHGINETEVKLVVCT----VDMLPKIKKVIKSCPQIGVVIVMEANTGKTIDLKPYENNNVEFIM-FRDLITRKSNEDRYPAPTPRDPAIIMYTSGSTGTPKGVVISHKNIISGVKALMNVVTFKPSDRYMGYLPLAHVLELISE-MVCLICGVKIGYSSPLTLTNKSSKVKRGCKGDANVFKPTLMCSVPLVLERIYKSVVDTMRRQG--------WAIEELFHYFVAYKMKWQXRGFDTPLLNKTLFRKXRYFIGGRVRLLISGGXPISHDTQSVSRTALCVPVLQ-----GYGLTELTGGVTMSDPYDRTTCRVGPPLLGLKLKLVDWEEGNYTINDKSFPRGEIHCGGDSISIGYFKNPEKTAEDFYEEDGIRWFKTGDIGQMEADGVLKLVDRKKDLVKLSGGEYISYGKVESILK 1884          

BLAST of amp dependent ligase vs. L. salmonis genes
Match: EMLSAG00000009904 (supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-1 gene:EMLSAG00000009904 transcript:EMLSAT00000009904 description:"maker-LSalAtl2s645-snap-gene-2.24")

HSP 1 Score: 60.8474 bits (146), Expect = 8.087e-10
Identity = 57/186 (30.65%), Postives = 83/186 (44.62%), Query Frame = 0

Query:  377 GAATLPIDTFEYFQSL-GMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPD---------------SNGVGEISTRSRNVFMGYINEELQTIQTFTPEG-WFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVG-EGRHF---LACLITP 541
            G+A+LP      +Q++ G  L +  G TE       +L   K  PG VG P+  V   I   +               S+  GE+  R  +VF  Y   E  T ++F P G WF +GD   ++  G   I GR    I+ SGG  ++ + IE R   + P+ IS + VVG E   F   +A +I P
Sbjct:  280 GSASLPEPLLRKWQTISGHILLERYGMTEIGMGLGNSLEEDKRLPGFVGTPFPNVEARIMSEEGSVLAYSNDVEFSYLSDKSGELQIRGPSVFSFYYGNEKATKESFDPNGDWFLTGDTASMNSRGVFKILGRTSADIIKSGGFKLSALEIE-REYLSYPD-ISEIAVVGLEDEIFGEIVAAIIVP 463          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|82209800|sp|Q7ZYC4.1|ACBG2_XENLA (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG2; AltName: Full=Acyl-CoA synthetase bubblegum family member 2)

HSP 1 Score: 432.95 bits (1112), Expect = 1.570e-141
Identity = 239/650 (36.77%), Postives = 377/650 (58.00%), Query Frame = 0

Query:   45 VKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSR-----VTLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYSE 653
            VK+R+ED+   +LPP+ +  + +E+V ++GD  AL++K  D  W   +++ YY   R  A  F+ LGL+   GVGI+  NS EW I+ V +I+AGG + GIYTT+S +   YVA N   +I+V+ENQ  L  +LQ   ++  LP +K I+  + ++ +  ++  + +WK+ ++LG+                                    L HDNITWT+      V L +    +E +++YLPLSH+AA M+D +++   G   YFA   ALKGSL   LREVRPT F+ VPRVWEK+Q KM+   +++S +K+ ++ WAK  G    +++  G     + Y +A+KLV  +V +ALGLDR       Y+GAA +  DT E+F SL + + +L G +E++GP T +L     +  + GK   G +  I  PDS+G GEI    R+VFMGY+N E +T ++   EGW  SGD+G+ DE G L I GRIKELI+ +GG+NI P+P E  +K+ +P +ISN +++G+ + FL+ L+T +  V+  + +P + L      + ++     T+ +  D++     +  +   IQ G+   N  ++S  +KVQK+ +L ++FS+ G ELGPT+KLKRPV+ + Y   ID+ Y +
Sbjct:   93 VKLRLEDSDVASLPPVTIHQLFQETVNKYGDYVALASKQGD-QWHKMSYKQYYEQCRIAAKGFIKLGLERYHGVGILGFNSAEWFIADVGAIFAGGFAVGIYTTNSAEACHYVAQNCEANIIVVENQKQLQKILQ---VQDQLPHLKAIIQYKDELKE--KRPNLYTWKEFMQLGKDIPDSQLDQIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNITWTAAAAGKTVRLREATDLQEIVVSYLPLSHIAAQMIDIWLTMKYGGATYFAQPDALKGSLAITLREVRPTAFMGVPRVWEKMQEKMKAVGAKSSTIKRKMATWAKGVGLETNLKKMNGSTPHPMKYHVANKLVFKKVRKALGLDRCTK---CYTGAAPITKDTLEFFLSLNIPVYELYGMSESSGPHTISLPD-AFRITSCGKVISGCKTKIHQPDSDGSGEILFWGRHVFMGYLNMEDKTHESLDEEGWLHSGDIGKHDENGFLYITGRIKELIITAGGENIPPVPTEDAVKEQVP-IISNAMLIGDKKKFLSMLLTLKCNVNADTGEPEDELTPEAIQFCRQIGSKATLVS--DIVGG--KDTAVYAAIQEGVNSVNEKSTSNAQKVQKWLILEKDFSITGGELGPTMKLKRPVVAKMYKDQIDSFYQD 727          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|166198360|sp|Q5ZKR7.2|ACBG2_CHICK (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG2; AltName: Full=Acyl-CoA synthetase bubblegum family member 2)

HSP 1 Score: 414.846 bits (1065), Expect = 2.885e-134
Identity = 237/656 (36.13%), Postives = 372/656 (56.71%), Query Frame = 0

Query:   45 VKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTS-----RVTLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKC-KPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWL-----KRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYSE 653
            VK+RM++   G+  P  V  + +E+V ++GD  AL++K  +G W   T++ YY      A +F+ L L+   GV I+  NSPEW I+ + +I+AGGL+ GIYTT+S +   YVA N + +ILV+EN T      +  E+   LP +K I+    ++ +     +  SW++ ++LG                                     L HDN+TWTS      + L +   ++E +++YLPLSHVAA M+D ++    G  V+FA   ALKG+L D LREVRPT FL VPRVWEKI+ KM+   +++S +++ ++ WAK  G +  ++   G  +  + + LA +LV  +V +A+GLDR       ++GAA +  +T E+F SL + + +L G +E++GP T ++  P+  +  + GK   G R  I  PD++G+GEI    R++FMGY+N E +T +    +GW  SGDLG+ D++G + I GRIKELI+ +GG+N+ P+PIE  +K+A P +ISN ++VG+   FLA L+T +  ++  S +P + L      +      K   V E I++    + A          IQ  +   N  A S  +K+QK+ VL ++FSV G ELGPT+KLKRPV+ +KY  +ID  Y++
Sbjct:   86 VKLRMDEEGMGSEAPKTVHEVFQEAVSKYGDYYALASK-KNGQWVKLTYKMYYDKCWKAAKSFLKLVLERFHGVCILGFNSPEWFIADIGAIFAGGLAVGIYTTNSPEACHYVAENCSANILVVENHT--QACRKSLEIEHKLPHMKAIIQYGEELKEKRPNQY--SWREFLDLGEDIPDSQLREIIESQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTSIAAGRSLMLLEATEKQELVVSYLPLSHVAAQMIDIWLPVTFGGQVFFAQPDALKGTLVDTLREVRPTAFLGVPRVWEKIEEKMKSVGAKSSTLRRKVASWAKGVGLQTNLKWMNGHSEVPMNFRLARQLVYKKVRKAIGLDRCTK---CFTGAAPISRETLEFFLSLNIPVFELYGMSESSGPHTVSI--PQAFRLTSCGKEMAGCRTLIHKPDADGIGEICFAGRHIFMGYLNMEEKTKEAIDKDGWLHSGDLGKCDKDGFIYITGRIKELIITAGGENVPPVPIEDAVKEACP-IISNAMLVGDKAKFLAMLLTLKCIINTESGEPGDDLTAEAIEYCQKLGSKATKVSEIISSKDKAVYA---------AIQAAVSEVNKRAVSNAQKIQKWVVLEKDFSVGGGELGPTMKLKRPVVAQKYKDLIDEFYAD 721          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|166198274|sp|A1L1K7.1|ACBG2_RAT (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG2; AltName: Full=Acyl-CoA synthetase bubblegum family member 2)

HSP 1 Score: 384.415 bits (986), Expect = 1.471e-123
Identity = 228/662 (34.44%), Postives = 365/662 (55.14%), Query Frame = 0

Query:   39 WNKHV--PVKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTS-----RVTLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKP----GTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLE-ESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYS 652
            W+ H    V +R+  + PG+  P+ +P + +ESVERFG   AL++K +   W + TF  YY   R  A + + LGLQ   GVGI+  NS EW+++++ +I AGGL  GIY T+S +  QYV     V++L++EN   L  +L     +  + TVK IV     +++    + + SW+  +ELG                                     L HDNITWT+      + L     +++ I++YLPLSH+AA ++D ++    G   +FA   AL+G+L   L+EV+PT FL VPRVWEK+Q  +++  +++S ++K    WAK  G +   ++ LG     + Y +A  LV ++V  +LGLD        +SGA+ L  D  E+F SL + + ++ G TE +GP T +     CK      + GK   G +N +   + +GVGE+    R+VFMGY+ +E  T++    +GW  SGD+GRLD    L I GRIKE+++ +GG+NI PIPIET +K+ +P +IS+ ++VG+   FL+ L+T + + D +S +P + L  E+++      +   T++    + + +     +   IQ G+++ N  A S   +++K+ +L ++FS+ G ELGPT KLKR +I +KY   IDNMYS
Sbjct:   24 WSTHCDGEVLLRLSKHGPGHETPMTIPELFQESVERFGAYPALASK-NGKKWDTLTFSQYYDVCRKAARSLIKLGLQRFHGVGILGFNSVEWVVAALGAILAGGLCVGIYATNSAEACQYVIKQANVNVLIVENDQQLQKILSIPPDK--METVKAIVQYRLPLME--NSTNLYSWQDFMELGNAIPNIQLDRVILSQKANQCAVIIYTSGTTGSPKGVMLSHDNITWTAGAMAREIELIHVSGKQDTIVSYLPLSHIAAQLMDIWIPIKVGVLTFFAQPDALRGTLVYTLQEVKPTYFLGVPRVWEKMQDTIKENVAKSSNLRKKAFAWAKMLGLKVNTKKMLGKRDIPMNYRMAKALVFTKVRTSLGLDNC---HTFFSGASPLSQDVSEFFLSLDIPIGEIYGMTECSGPHTVS-----CKSIYRVLSCGKVLNGCKNMLYKQNKDGVGEVCMWGRHVFMGYLGKEDATLEVLDEDGWLHSGDIGRLDSHDFLYITGRIKEVLITAGGENIWPIPIETLVKEKIP-IISHAMLVGDKAKFLSMLLTLKCETDQMSGEPLDKLNLEAISFCQMLGSQAVTVSDILKIRDPV-----VYTAIQYGIDIVNQQAVSDSHRIRKWIILEKDFSIQGGELGPTSKLKRDLITQKYKAQIDNMYS 666          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|123779881|sp|Q2XU92.1|ACBG2_MOUSE (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG2; AltName: Full=Acyl-CoA synthetase bubblegum family member 2; AltName: Full=Bubblegum-related protein)

HSP 1 Score: 379.793 bits (974), Expect = 8.021e-122
Identity = 225/661 (34.04%), Postives = 359/661 (54.31%), Query Frame = 0

Query:   39 WNKHV--PVKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLP-----TVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTS-----RVTLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMY 651
            W+ H    V +R+  + PG+  P+ +P + +ES ERF    AL++K +   W + TF  YY   R  A + + LGLQ  + VGI+  NS EW+++++ +I AGGL  GIY T+S +  QYV     VSIL++EN   L  +L       ++P     TVK IV  +  +++     +  SW   +ELG                                     L HDNITWT+      + + +   ++  I++YLPLSH+AA + D ++    G   +FA   AL+G+L   L+EV+PT F+ VPR+WEK+Q  +++  +R+S ++K    WAK  G +   +R LG     + Y +A  LV ++V  +LGLD   A    +S A+ L  D  E+F SL + + ++ G +E +GP T +      +  + GK   G +N + + +  GVGE+    R+VFMGY+N+E  T++     GW  SGD+GRLD    L I GRIKE+++ +GG+N++PIPIET +K+ +P +IS+ ++VG+   FL  L+T + + D  S +P N L      + +   +    TT  D++++   +  +   IQ G+++ N  A S   +++K+ +L ++FS+ G ELGPT KLKR VI +KY   ID+MY
Sbjct:   24 WSTHCDGEVLLRLSKHGPGHETPMTIPELFQESAERFSAYPALASK-NGKKWDTLTFSQYYEMCRKAAKSLIKLGLQRFQCVGILGFNSVEWVVTALGTILAGGLCVGIYATNSAEACQYVIQQANVSILIVENDQQLQKIL-------LIPPDKMETVKAIVQYKLPLMESMANLY--SWNDFMELGNDIPNIQLDRVILSQKANQCAVILYTSGTTGTPKGVLLSHDNITWTAGAMSQEMEINRVSGKQNTIVSYLPLSHIAAQLTDIWIPIKIGALTFFAQPDALRGTLVYTLQEVKPTLFMGVPRIWEKMQDTIKENVARSSRLRKKAFAWAKMLGLKVNTKRMLGKRDIPMNYRMAKALVFAKVRTSLGLDNCHA---FFSSASPLSQDVSEFFLSLDIPIGEIYGMSECSGPHTVSNKSVY-RVLSCGKVLSGCKNMLYNQNKEGVGEVCMWGRHVFMGYLNKEEATLEALDENGWLHSGDIGRLDSHDFLYITGRIKEILITAGGENVSPIPIETLVKEKIP-IISHAMLVGDKAKFLCMLLTLKCETDRKSGEPLNKLSVEAKSFCQM--LGSQATTVSDILKS--RDQVVYTAIQYGIDIVNQQAMSDSHRIRKWIILEKDFSIQGGELGPTSKLKRSVITQKYKAQIDSMY 665          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|296434386|sp|Q5FVE4.2|ACBG2_HUMAN (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG2; AltName: Full=Acyl-CoA synthetase bubblegum family member 2; AltName: Full=Bubblegum-related protein; AltName: Full=PRTD-NY3)

HSP 1 Score: 373.244 bits (957), Expect = 2.607e-119
Identity = 220/650 (33.85%), Postives = 357/650 (54.92%), Query Frame = 0

Query:   45 VKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQ---PHQKSF-VMSWKQLVELGR------------------------------------LHHDNITWTSRVTLTQYEW--EKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLE-ESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMY 651
            V +R+  + PG+  P+ +P   +ESV RFG   AL++K +   W+   F  YY   R  A + + LGL+   GVGI+  NS EW I++V +I AGGL  GIY T+S +  QYV  +  V+IL++EN          ++L+K+L   +  +     IIQ   P +K+  + SW   +ELGR                                    L HDNITW +      ++   + E +++YLPLSH+AA M+D ++    G   YFA   ALKG+L   L+EV+PT F+ VP++WEKI   ++  ++++ G+KK    WA+  G +   ++ LG     + Y +A  LV S+V  +LGLD         SG A L  +T E+F SL + + +L G +E++GP T +      +  + GK   G +N +   + +G+GEI    R++FMGY+  E +T +    EGW  SGDLG+LD  G L + G IKE+++ +GG+N+ PIP+ET +K  +P +ISN ++VG+   FL+ L+T + +++ +S +P + L  E++       +   T+T   ++++  Q +  + + IQ G+   N  A +  ++++K+ +L ++FS+ G ELGP +KLKR  + +KY K ID+MY
Sbjct:   36 VLLRLSKHGPGHETPMTIPEFFRESVNRFGTYPALASK-NGKKWEILNFNQYYEACRKAAKSLIKLGLERFHGVGILGFNSAEWFITAVGAILAGGLCVGIYATNSAEVCQYVITHAKVNILLVEND---------QQLQKILSIPQSSLEPLKAIIQYRLPMKKNNNLYSWDDFMELGRSIPDTQLEQVIESQKANQCAVLIYTSGTTGIPKGVMLSHDNITWIAGAVTKDFKLTDKHETVVSYLPLSHIAAQMMDIWVPIKIGALTYFAQADALKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKTLVFSKVKTSLGLDHC---HSFISGTAPLNQETAEFFLSLDIPIGELYGLSESSGPHTISNQN-NYRLLSCGKILTGCKNMLFQQNKDGIGEICLWGRHIFMGYLESETETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIP-IISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVT---EIVK--QQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELGPMMKLKRHFVAQKYKKQIDHMY 665          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|81903322|sp|Q99PU5.1|ACBG1_MOUSE (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG1; AltName: Full=Acyl-CoA synthetase bubblegum family member 1; Short=mBG1; AltName: Full=Gonadotropin-regulated long chain acyl CoA synthetase; Short=GR-LACS; AltName: Full=Lipidosin)

HSP 1 Score: 370.548 bits (950), Expect = 1.262e-117
Identity = 225/660 (34.09%), Postives = 362/660 (54.85%), Query Frame = 0

Query:   39 WNKHVPVKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSRV-----TLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKK---EGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGP-KCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYSE 653
            W      ++R+         P  V  M  E+++++G+  AL  K  D  W+  ++  YY   R VA  F+ LGL+    V I+  NSPEW  S+V +++AGG+  GIYTTSS +  QY++++   +++V++ Q  L+ +L+   + K LP +K +V+ +     P + + V + ++L+ELG+                                    L  DNITWT+R       +   E ++E +++YLPLSH+AA + D +     G  V FAD  ALKG+L + LREV PT  + VPRVWEKI  ++Q+ A+++  +++ +  WA     E    C    L P    L    A  LVL+RV QALG   A  +   Y GAA +  +T  +F  L + L    G +E+TGP    ++ P   +  + G+   G R  + + D++G+GEI    R +FMGY+N E +T +    EGW  +GD+GRLD +G L I GR+KELI+ +GG+N+ P+PIE  +K  LP +IS+ +++G+ R FL+ L+T +  +D  +++PT+ L E    + +R  V    +T  +++   Q +  + Q I  G++  NA+A++RP  +QK+ +L ++FS+ G ELGPT+KLKR  + EKY  +ID+ Y E
Sbjct:   79 WTTRADGRVRLRLEPFCTQRPYTVHQMFYEALDKYGNLSALGFKRKD-KWERISYYQYYLIARKVAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYISHDCRANVIVVDTQKQLEKILK---IWKDLPHLKAVVIYQEP--PPKKMANVYTMEELIELGQEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFADPDALKGTLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPSNDLKPFTSRL----ADYLVLARVRQALGF--AKCQKNFY-GAAPMTAETQRFFLGLNIRLYAGYGLSESTGPHF--MSSPYNYRLYSSGRVVPGCRVKLVNQDADGIGEICLWGRTIFMGYLNMEDKTCEAIDSEGWLHTGDMGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELP-IISSAMLIGDQRKFLSMLLTLKCTLDPETSEPTDSLTEQAVEFCQR--VGSKASTVSEIVG--QRDEAVYQAIHEGIQRVNANAAARPYHIQKWAILQRDFSISGGELGPTMKLKRLTVLEKYKDIIDSFYQE 718          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|74947350|sp|Q9V3S9.1|BGM_DROME (RecName: Full=Very long-chain-fatty-acid--CoA ligase bubblegum)

HSP 1 Score: 367.851 bits (943), Expect = 2.736e-117
Identity = 230/668 (34.43%), Postives = 351/668 (52.54%), Query Frame = 0

Query:   32 KATDFISWNKHVPVKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQK--SFVMSWKQLVELG------------------------------------RLHHDNITWTSRVTLTQYEW---EKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKK-EGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKP-----GTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYS 652
            +A  + + N+   VKIRM  +  G   PI VP ++K +V  +GD  AL TK     + + T++ Y   V  VA AF+ LGL+    VG++A N  EW  S++ +I A G+  GIYTT+S   VQ+V  ++   I+V+++   +D +     +R  LP +K  + ++ +   P+ K       W ++  +                                      L HDNIT+  R  +   +      E I++YLPLSHVAA  VD Y        ++FADK ALKG+L   L++ RPTRF+ VPRV+EK Q +M   AS +  +KK ++ WAK    + Y + +  G       Y +A  L++S+V QALG DR +    L S AA +  +T +YF SL +++ D  G +ET G  T       C P      T+GK   G  +   + D+NG GE+  R R+VFMGYI+ + +T ++   + W  SGDLG +D++G + + GR KE+I+ +GG+NI P+ IE  IK  L + ISN  +VGE R +L  LIT + +VD  S +P + L    +VW+K   V     +    I A      + + I+  ++ AN  + S  +KVQKF +LP +FS+   ELGPTLK+KR V+ + YA  I+ +Y+
Sbjct:   18 QADAYRTTNRQDAVKIRMAKDGIGAEEPISVPGLLKRTVNNYGDYPALRTKNGKNGYHTVTYKQYEQKVHQVAKAFIKLGLEEHHSVGVLAFNCAEWFYSAMGAIHARGIIAGIYTTNSADAVQHVLESSHAQIVVVDDAKQMDKI---HAIRDKLPKLKAAIQIQ-EPYSPYLKKEDGYYRWSEIESMNVSDVEDQYMTRLENVAINECCCLVYTSGTVGMPKGVMLSHDNITFDVRGIVKAMDRVVVGAESIVSYLPLSHVAAQTVDIYTCAFVAGCIWFADKDALKGTLVKSLQDARPTRFMGVPRVYEKFQERMVAVASSSGSLKKMLASWAKGITLKHYMVSQ--GKSSGGFRYKIAKSLIMSKVKQALGFDRVLT---LASAAAPMSPETKKYFLSLDLKIVDAFGMSETAGCHTI------CLPDSVGLNTIGKTLPGCESKFINKDANGHGELCIRGRHVFMGYIDNKEKTEESLDDDCWLHSGDLGFVDDKGYVSLTGRSKEIIITAGGENIPPVHIENTIKKEL-DAISNAFLVGEQRKYLTVLITLKTEVDKDSGEPLDELSHESSVWVKSLGVEHKTVSD---ILAAGPCPKVWKSIEDAIKRANKQSISNAQKVQKFTILPHDFSIPTGELGPTLKVKRNVVSKMYADEIEKLYA 666          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|81902685|sp|Q924N5.1|ACBG1_RAT (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG1; AltName: Full=Acyl-CoA synthetase bubblegum family member 1; AltName: Full=Gonadotropin-regulated long chain acyl CoA synthetase; Short=GR-LACS)

HSP 1 Score: 369.007 bits (946), Expect = 4.155e-117
Identity = 229/679 (33.73%), Postives = 370/679 (54.49%), Query Frame = 0

Query:   25 LSDPVDSKATDFIS-----WNKHVPVKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSRV-----TLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKK---EGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGP-KCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYSE 653
            LS P  ++A    +     W      ++R+         P  V  M  E+++++G+  AL  K  D  W+  ++  YY   R VA  F+ LGL+    V I+  NSPEW  S+V +++AGG+  GIYTTSS +  QY+A++   +++V++ Q  L+ +L+   + K LP +K +V+ +     P + + V + ++L+ELG+                                    L  DNITWT+R       +   E ++E +++YLPLSH+AA + D +     G  V FAD  ALKG+L + LREV PT  + VPRVWEKI  ++Q+ A+++  +++ +  WA     E    C    L P    L    A  LVL+RV QALG   A  +   Y GAA +  +T  +F  L + L    G +E+TGP    ++ P   +  + G+   G R  + + D++G+GEI    R +FMGY+N E +T +    EGW  +GD+GRLD++G L I GR+KELI+ +GG+N+ P+PIE  +K  LP +IS+ +++G+ R FL+ L+T +  ++  +++PT+ L E    + +R  V    +T  +++   Q +  + Q I  G++  NA+A++RP  +QK+ +L ++FS+ G ELGPT+KLKR  + EKY  +ID+ Y E
Sbjct:   60 LSAPEKARAASLDASEEALWTTRADGRVRLRLEPFCTQLPYTVHQMFYEALDKYGNLSALGFKRKD-KWERISYYQYYLIARKVAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYIAHDCRANVIVVDTQKQLEKILK---IWKDLPHLKAVVIYQEP--PPKKMANVYTMEELIELGQEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFADPDALKGTLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPSNDLKPFTSRL----ADYLVLARVRQALGF--AKCQKNFY-GAAPMTAETQRFFLGLNIRLYAGYGLSESTGPHF--MSSPYNYRLYSSGRVVPGCRVKLVNQDADGIGEICLWGRTIFMGYLNMEDKTHEAIDSEGWLHTGDMGRLDDDGFLYITGRLKELIITAGGENVPPVPIEEAVKMELP-IISSAMLIGDQRKFLSMLLTLKCTLNPETSEPTDNLTEQAVEFCQR--VGSKASTVSEIVG--QKDEAVYQAIHEGIQRVNANAAARPYHIQKWAILERDFSISGGELGPTMKLKRLTVLEKYKDIIDSFYQE 718          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|296434385|sp|Q96GR2.2|ACBG1_HUMAN (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG1; AltName: Full=Acyl-CoA synthetase bubblegum family member 1; Short=hBG1; Short=hsBG; Short=hsBGM; AltName: Full=Lipidosin)

HSP 1 Score: 360.147 bits (923), Expect = 1.318e-113
Identity = 226/654 (34.56%), Postives = 358/654 (54.74%), Query Frame = 0

Query:   45 VKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSRV-----TLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKK---EGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGP-KCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYSE 653
            V++R++ + P    P  V  M  E+++++GD  AL  K  D  W+  ++  YY   R  A  F+ LGL+    V I+  NSPEW  S+V +++AGG+  GIYTTSS +  QY+A +   ++++++ Q  L+ +L+   + K LP +K +V+ +     P++ + V + ++ +ELG                                     L  DNITWT+R       +   E ++E +++YLPLSH+AA + D +     G  V FA+  ALKGSL + LREV PT  + VPRVWEKI  ++Q+ A+++  +++ +  WA     E    C    L P    L    A  LVL++V QALG   A  +   Y GAA +  +T  +F  L + L    G +ET+GP    ++ P   +  + GK   G R  + + D+ G+GEI    R +FMGY+N E +T +    EGW  +GD GRLD +G L I GR+KELI+ +GG+N+ P+PIE  +K  LP +ISN +++G+ R FL+ L+T +  +D  ++  T+ L E    + +R  V    TT  ++IE  + +  + Q I+ G+   N +A++RP  +QK+ +L ++FS+ G ELGPT+KLKR  + EKY  +ID+ Y E
Sbjct:   90 VRLRIDPSCPQ--LPYTVHRMFYEALDKYGDLIALGFKRQD-KWEHISYSQYYLLARRAAKGFLKLGLKQAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYIAYDCCANVIMVDTQKQLEKILK---IWKQLPHLKAVVIYKEP--PPNKMANVYTMEEFMELGNEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIRPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPGSDLKPFTTRL----ADYLVLAKVRQALGF--AKCQKNFY-GAAPMMAETQHFFLGLNIRLYAGYGLSETSGPHF--MSSPYNYRLYSSGKLVPGCRVKLVNQDAEGIGEICLWGRTIFMGYLNMEDKTCEAIDEEGWLHTGDAGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELP-IISNAMLIGDQRKFLSMLLTLKCTLDPDTSDQTDNLTEQAMEFCQR--VGSRATTVSEIIE--KKDEAVYQAIEEGIRRVNMNAAARPYHIQKWAILERDFSISGGELGPTMKLKRLTVLEKYKGIIDSFYQE 721          

BLAST of amp dependent ligase vs. SwissProt
Match: gi|166198363|sp|Q4R4P9.2|ACBG1_MACFA (RecName: Full=Long-chain-fatty-acid--CoA ligase ACSBG1; AltName: Full=Acyl-CoA synthetase bubblegum family member 1)

HSP 1 Score: 357.836 bits (917), Expect = 1.138e-112
Identity = 222/655 (33.89%), Postives = 354/655 (54.05%), Query Frame = 0

Query:   45 VKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSRV-----TLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKK---EGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGP-KCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVG-EGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYSE 653
            V++R++ + P    P  V  M  E+++++GD  AL  K  D  W+  ++  YY   R  A  F+ LGL+    V I+  NSPEW  S+V +++AGG+  GIYTTSS +  QY+A +   ++++++ Q  L+ +L+   + K LP +K +V+ +     P++ + V + ++ +ELG                                     L  DNITWT+R       +   E ++E +++YLPLSH+AA + D +     G  V FA+  ALKGSL + LREV PT  + VPRVWEKI  ++Q+ A+++  +++ +  WA     E    C    L P    L    A  LVL++V QALG  +       + GAA +  +T  +F  L + L    G +ET+GP    ++ P   +  + GK   G R  + + D+ G+GEI    R +FMGY+N E +T +    EGW  +GD GRLD +G L I GR+KELI+ +GG+N+ P+PIE  +K  LP +ISN +++G + R FL+ L+T +  +D  ++ PT+ L E    + +R     T  +     E +  +  + Q I+ G+   N +A++RP  +QK+ +L ++FS+ G ELGPT+KLKR  + EKY  +ID+ Y E
Sbjct:   90 VRLRIDPSCPQL--PYTVHRMFYEALDKYGDFSALGFKCQD-KWEHISYSQYYLLARRAAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYIAYDCCANVIMVDTQKQLEKILK---VWKQLPHLKAVVIYKEP--PPNKMANVYTMEEFMELGNEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIRPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIRRKMLLWAMSVTLEQNLTCPGSDLKPFTTRL----ADYLVLAKVRQALGFAKCQKN---FYGAAPMTAETQHFFLGLNIRLYAGYGLSETSGPHF--MSSPCNYRLYSSGKLVPGCRVKLVNQDTEGIGEICLWGRTIFMGYLNMEDKTCEAIDEEGWLHTGDAGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKMELP-IISNAMLIGTDQRKFLSMLLTLKCTLDPDTSDPTDNLTEQAVEFCQRVGSRATTVS-----EIVGKDEAVYQAIEEGIRRVNMNAAARPYHIQKWAILERDFSISGGELGPTMKLKRLTVLEKYKDIIDSFYRE 721          

BLAST of amp dependent ligase vs. nr
Match: gi|1325316200|ref|XP_023339811.1| (long-chain-fatty-acid--CoA ligase ACSBG2-like isoform X1 [Eurytemora affinis])

HSP 1 Score: 475.707 bits (1223), Expect = 6.988e-156
Identity = 264/648 (40.74%), Postives = 387/648 (59.72%), Query Frame = 0

Query:   45 VKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSRVTLTQYEW--EKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKD---GLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMY 651
            V+IR  ++      P  V TM+ +++ R  DR AL+ K  DG W +W+F +Y +D+RSVA AF+ LGL+    V I+  N+PEW IS++AS+ AGGL+ GIYTT+S+ +V+YVA ++  +I+VLE+Q  LD +L    ++  L  ++ IV        P     V+SW++L+++GR                                    +  DNITWT       Y+W  + E  ++YLPLSHVAA ++D Y+S  GG T+ FADK AL+GSL + L+EV PT+FL VPRVWEKI+ K+++    NSG+KK++  WAK    ++   +  G    S+ YT+A  L+L ++H ALGL+ A   D   G +S AA L   TF+YF SL M + +LLG +ET GPQT  L G   + G+VG+ Y      I  PD +G+GEI TR RNV MGY+ +E +T +    +GW  SGDLGR+DE+G + +CGR+KELI+  GG+NIAP+PIE  ++  L E+ S+V+V+G+ R  L+ +IT +  +D   NQPT+ L   V  WL   N+    TT+ +LI+  + N  ++  I   ++  N HA S  ++V KF + P+EFS+ G EL PT+K+KR  + EKY   ID +Y
Sbjct:   70 VEIRTNNHPSSRWKPKSVWTMLNDTLARVPDRTALAVK-RDGIWVTWSFTEYINDIRSVAKAFINLGLKQHHAVNIIGFNAPEWHISNIASVVAGGLATGIYTTNSQDSVRYVAQHSRANIIVLEDQEQLDKILN---IQDQLKELQCIVQYSGTPTVPG----VLSWEELLDIGRGLPDSVLQERLAMQAINQPCMLVYTSGTTGNPKGVMISQDNITWTVNAVQGMYDWKMDCEHGISYLPLSHVAAQIIDIYLSAYGGATISFADKEALQGSLINTLKEVNPTKFLGVPRVWEKIEEKLKELGKNNSGIKKTVMDWAKNAALQHHTDKMAGKRGQSISYTVARWLILRKIHAALGLEVAGHPDNNGGFFSSAAPLSPQTFQYFLSLDMPILELLGSSETGGPQTACLPGAGMRVGSVGRSYPHFETVILKPDKDGIGEICTRGRNVCMGYLWDEEKTKELIDEDGWVHSGDLGRIDEDGFIYVCGRMKELIITGGGENIAPVPIEDLLRAELEEICSHVMVIGDARKHLSVIITLKTVLDS-RNQPTSKLHPDVQTWL--TNLGSEATTAVELIK--EDNDEVKAAILKSIKTVNKHAVSNAQRVHKFMIAPEEFSLQGGELTPTMKVKRHFVLEKYKNEIDALY 704          

BLAST of amp dependent ligase vs. nr
Match: gi|514692819|ref|XP_004993982.1| (AMP dependent ligase [Salpingoeca rosetta] >gi|326428131|gb|EGD73701.1| AMP dependent ligase [Salpingoeca rosetta])

HSP 1 Score: 456.062 bits (1172), Expect = 3.038e-149
Identity = 253/650 (38.92%), Postives = 387/650 (59.54%), Query Frame = 0

Query:   45 VKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLV---------ELGR---------------------------LHHDNITWTSR---VTLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEG--RRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLE-ESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYS 652
            V +++  +   +  PI V    KE+VE+ GDR AL     DG+W+ WT++D+Y DV+    AF+ LGL +   VGI+  NSPEW I+ + +I+AGG + GIYTT+  + ++YV N++   I+V E++  LD +LQ   + +    +K IVL    +        ++ W + +         ELG                            + HDN+TWTS     +L    + KE I++YLPLSH+AA ++D +   + G TV+FA   ALKG+L   L+EVRPT FL VPRVWEKI+ KM+   S  +G+KK I  WAK++G    Y IQ+  G   PS G+ +A KLV S+V +ALGLDR        + AA +  DT EYF S+ + + ++ G +E TGPQ+ N+ G K + G+VG P  G    I +PD +G GEI  R R++  GY+  E +T ++   EGW  SGD+GR+DE+G L I GRIKE+I+ +GG+N+AP+PIE  +K+  P +ISN +++G+ R FL+ L+  + +VD  +  P + L  +++ +   + +  +T+T + D       + H+ + IQ G++ AN HA SR +KVQKF++LP++FSV G ELGPTLKL+RP++ ++Y ++ID+ Y+
Sbjct:   14 VDLKITPDGAASAEPITVMQRFKETVEKVGDRPALCVS-RDGAWQQWTWRDFYEDVKMAGRAFIKLGLDAHHAVGIIGFNSPEWFIADLGAIFAGGFATGIYTTNGPEAIEYVVNHSKTQIIVAEDKKQLDKILQ---VHQNFEHLKAIVLYNDTVPDVECTVPILDWDEFMRHGADVPDFELGHRMSVQKPGQCCMLIYTSGTTGNPKGVMITHDNVTWTSATLAASLPNMTFGKEHIVSYLPLSHIAAQILDMHAPMILGATVWFAQPTALKGTLVQTLQEVRPTVFLGVPRVWEKIEEKMRAVGSETTGLKKKIGTWAKRKGLDGAYAIQQ--GRPVPS-GWGVAKKLVFSKVRKALGLDRC---KFFATAAAPISKDTLEYFLSINIPIMEVYGMSENTGPQSLNIPG-KHRTGSVGLPLAGTELKIHEPDQDGNGEICFRGRHICKGYMYNEEKTRESIDAEGWLHSGDIGRVDEDGYLYITGRIKEIIITAGGENVAPVPIENLVKENCP-IISNCMLIGDRRKFLSLLVCLKSEVDPETTAPLDELTPQTIEILEAQGSKAKTVTEAMD-------DEHVLKYIQAGIDEANKHAVSRAQKVQKFKILPRDFSVPGGELGPTLKLRRPIVVKQYEELIDSFYA 644          

BLAST of amp dependent ligase vs. nr
Match: gi|167534933|ref|XP_001749141.1| (hypothetical protein [Monosiga brevicollis MX1] >gi|163772294|gb|EDQ85947.1| predicted protein [Monosiga brevicollis MX1])

HSP 1 Score: 451.825 bits (1161), Expect = 2.237e-147
Identity = 248/640 (38.75%), Postives = 379/640 (59.22%), Query Frame = 0

Query:   55 GNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSF---VMSWKQLVELGR------------------------------------LHHDNITWTSRVTLTQYEWE---KEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYS 652
             ++ P+ V    KE+VERFGDR AL+ K  DG W +WT++DY+ D+ + A AF+  GL     VGI+  NSPEW I+ + +++AGG + GIYTT+  +++QYVA+++    L +E++  L  +   +E++  LPTVK +V+    + +  +K     V+ W + +  GR                                    + HDNITWT++   T    +   +E I++YLPLSH+AA ++D +   L G  V+FA   ALKGSLP  L EVRPT FL VPRVWEKI+ KM+   ++++G+KK I+ WAK++G    +  + G  +PS G+ LA+KLV  ++   LGLDR      L + AA +  +T +YF SL + + ++ G +E TGPQT N +G     G+VG    G+   I +PD+NG GEI  R R+V  GY+  E +T +T   +GW  +GD+GR+D+ G L I GRIKE+I+ +GG+N+AP+PIE  +K   P ++SNV+V+G+ R FL+ ++T + +VD+ S +P + L + V        +  +I +S   + A + +  + + I    + AN  + SR + VQK  +LP +FSV G ELGPTLKLKRPV++EKY  VID +Y+
Sbjct:   30 ASIKPVTVMERFKETVERFGDRDALAVK-RDGYWHTWTYRDYHRDIITAAKAFIHFGLDRHHSVGIIGFNSPEWFIADLGAVFAGGFASGIYTTNGPESLQYVADHSRSQFLFVEDRKQLAKI---KEVQDQLPTVKLVVMWGEPVEEKDRKDLKIKVIGWDEFMRGGRDVAEHEVHNRMKVQSPGECCTLIYTSGTTGTPKAVMMTHDNITWTAKALSTHARLDDHRQEHIISYLPLSHIAAQILDMHAPILMGAKVWFAQPDALKGSLPTTLAEVRPTIFLGVPRVWEKIEEKMRIVGAQSTGLKKKIATWAKRKGMEASMADQRGERRPS-GFGLANKLVFKKIRAKLGLDRC---HFLATAAAPISRETLDYFLSLYLPIMEVYGMSENTGPQTVNRSGNHTT-GSVGVTMAGLETKIDNPDANGDGEICMRGRHVMKGYLFNEEKTRETIDEDGWLHTGDVGRMDDRGFLFITGRIKEIIITAGGENVAPVPIEDTMKACCP-ILSNVMVIGDKRKFLSQVVTLKCEVDVESGEPLDKLTDQVI------EILSSIGSSATTVSAAREDPKVIEYIDAKRKEANNKSVSRAQNVQKSYILPVDFSVPGGELGPTLKLKRPVVYEKYKDVIDGLYA 653          

BLAST of amp dependent ligase vs. nr
Match: gi|632966016|ref|XP_007899185.1| (PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2 [Callorhinchus milii])

HSP 1 Score: 449.129 bits (1154), Expect = 2.858e-145
Identity = 253/650 (38.92%), Postives = 377/650 (58.00%), Query Frame = 0

Query:   45 VKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTS-----RVTLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYSE 653
            VK+RME +     PPI V  M  E+V ++GD  AL++K  D  WK  TF+ YY + R+ A +FV LGL+   GVGI+  NSPEW I+ + +I AGGL+ GIYTT+S +   YVA N+  +ILV+EN   L  +LQ   ++  LP +K IV  + ++ +  ++  V +W   +++GR                                    L HDN+TWT+      V L      +E +++YLPLSH+AA M+D ++S   G   YFA   ALKGSL + +REVRPT F+ VPRVWEK+Q KM+   +++S V++ ++ WAK  G +  + R  G +   L + +A KLV  RV +ALGL R       Y+GAA +  DT E+F SL + + +L G +E++GP T ++    C+  + GK   G +  I  PDS+  GEI    R+VFMGY+N E +T ++   EGW  SGDLG+ DE+G L I GRIKELI+ +GG+NI P+PIE  IK+ +  +ISN +V+G+ R FL+ L+T +  V+  +  P + L   VT + KR  +    T   +++ +   +  +   IQ G+   N  A+S  +++QK+ +L ++FS+ G ELGPT+KLKRPVI + Y   ID+ Y +
Sbjct:   94 VKLRMESSGLAADPPITVHQMFVETVTKYGDYMALASKQGD-QWKKLTFKQYYDECRNAAKSFVKLGLERFHGVGILGFNSPEWFIADIGAILAGGLAVGIYTTNSPEACHYVAENSEANILVVENNKQLQKILQ---IQDQLPHLKAIVQYKDEVKE--KRPNVYTWADFMQVGRSVSDSELDEIIASQKPNQCCTLVYTSGTTGKPKGVMLSHDNLTWTAGVAGNTVNLRAATDSQESVISYLPLSHIAAQMIDIWLSIKCGGATYFAQPDALKGSLVNTMREVRPTAFMGVPRVWEKMQEKMKTVGAKSSTVRRKVASWAKDVGLQTNLNRMNGNMSQPLNFRVAKKLVFKRVRKALGLSRCTK---CYTGAAPITKDTIEFFLSLNIPVYELYGMSESSGPHTISVP-ESCRLSSCGKVMPGCKTLIYKPDSDSCGEICFWGRHVFMGYLNMEDKTEESIDEEGWLHSGDLGKHDEDGFLYITGRIKELIITAGGENIPPVPIEDAIKEQI-SVISNAMVIGDKRKFLSVLLTLKCNVNSDTGDPLDDLTPEVTQFCKR--IGSKATKVSEILAS--KDRDVFNAIQEGINRVNEKATSNAQRIQKWIILEKDFSIPGGELGPTMKLKRPVIMKMYPDQIDDFYKD 728          

BLAST of amp dependent ligase vs. nr
Match: gi|1007783546|ref|XP_015828743.1| (PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2-like isoform X2 [Nothobranchius furzeri])

HSP 1 Score: 445.277 bits (1144), Expect = 8.676e-144
Identity = 254/660 (38.48%), Postives = 391/660 (59.24%), Query Frame = 0

Query:   33 ATDFISWNKHVPVKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSRVT--LTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHH--LRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYS 652
            A +  S ++   V++RME++ PG+  P+ +  M  E+VE  GD+ AL +K +DG W + T+++YY + R+ A +F+ LGL+   GVGI+  NSPEW IS V  I+AGGL+ GIYTT+S +  QYVA N    +LV+ENQ  LD +L+   ++  LP +K I+  + ++ Q  +  F+ +W + ++LG                                     L HDNI+WT+R    +    + +E +++YLPLSHVAA M+D +     G T+YFAD  ALKGSL + L+EVRPT FL VPRVWEK+Q KM+ A S+ S +KK ++ WAK  G +Y      G      G+ L++ LV  RV   LGLDR        +GAA +  DT EYF SL + +++L G +E++GP T ++   + + G+ GK   G +  +  PD +G GEI    R+VFMGY+N   +T++    +GW  SGDLG+ D++  L I GRIKELI+ +GG+NI P+PIE  +K  +P +ISN +++G+   FL+ ++T + ++D  + +PT+ L   V  + +++ V  T       +  + +N    + + IQ G+E  NA ++S  +KVQK+ VL ++FSV+G ELGPTLKL+RPV+ + Y   I+ +Y+
Sbjct:   91 AKELWSSSRDKAVRLRMENSGPGSETPMTIHQMFLETVEWCGDKPALVSK-TDGRWVTLTWREYYEECRTAAKSFLKLGLERFHGVGILGFNSPEWFISDVGCIFAGGLATGIYTTNSPEACQYVAANCEADVLVVENQKQLDKILK---VKGQLPHLKAIIQYKGELQQ--KAPFLYTWAEFMKLGEDVSNEQLNAVIDSLRSNECCSLIYTSGTTGNPKGVMLSHDNISWTARTVFKMINENYGEEVLVSYLPLSHVAAQMIDIWGCMSLGGTIYFADPDALKGSLVNTLKEVRPTGFLGVPRVWEKMQEKMKAAGSKASPLKKRLADWAKSVGLQYNYSVMNGENAVPWGFMLSNNLVFKRVRAGLGLDRCTF---CCTGAAPITKDTLEYFMSLNIPVKELYGMSESSGPHTVSIN--QYRIGSCGKVMPGCKTKLDHPDEDGNGEICFWGRHVFMGYLNMPDKTVEALDEDGWLHSGDLGKHDKDDFLYITGRIKELIITAGGENIPPVPIEDAVKTEVP-IISNAMLIGDKLKFLSMMLTLKCEMD-DNGEPTDDLSPEVLDFCQQHGVKST------KVSEVLANKEPAIYKAIQEGMERVNARSTSNAQKVQKWIVLGRDFSVVGGELGPTLKLRRPVVVKMYQDEINELYT 731          

BLAST of amp dependent ligase vs. nr
Match: gi|847085637|ref|XP_012812199.1| (PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2 isoform X3 [Xenopus tropicalis] >gi|847085640|ref|XP_012812203.1| PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2 isoform X3 [Xenopus tropicalis] >gi|1043436686|gb|OCA49312.1| hypothetical protein XENTR_v90001538mg [Xenopus tropicalis] >gi|1043436687|gb|OCA49313.1| hypothetical protein XENTR_v90001538mg [Xenopus tropicalis])

HSP 1 Score: 439.499 bits (1129), Expect = 1.343e-142
Identity = 242/650 (37.23%), Postives = 379/650 (58.31%), Query Frame = 0

Query:   45 VKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSR-----VTLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYSE 653
            V++RMED+   +LPP+ +  + +++V+++GD  AL++K  D  W   T++ YY   R  A  F+ LGL+   GVGI+  NS EW I+ V +I+AGG + GIYTT+S +   YVA N   +I+V+ENQ  L  +LQ   ++  LP +K I+  + ++ +  ++  + +WK+ ++LG+                                    L HDNITWT+      V L +    +E +++YLPLSH+AA M+D +++   G   YFA   ALKGSL + LREVRPT F+ VPRVWEK+Q KM+   +++S +K+ ++ WAK  G    +++  G     + Y +A KLV  +V +ALGLDR       Y+GAA +  DT E+F SL + + +L G +E++GP T +L     +  + GK   G +  I  PD++G GEI    R+VFMGY+N E +T ++   EGW  SGD+G+ DE G L I GRIKELI+ +GG+NI P+PIE  +K+ +P +ISN +V+G+ + FL+ L+T +  V+  + +P + L      + ++     T+ +  D++     +  +   IQ G+   N  A+S  +KVQK+ +L Q+FS+ G ELGPT+KLKRPV+ + Y   ID+ Y +
Sbjct:   12 VELRMEDSGVASLPPVTIHQLFQDTVKKYGDYVALASKQGD-QWHKMTYEQYYEQCRIAAKGFLKLGLERYHGVGILGFNSAEWFIADVGAIFAGGFAVGIYTTNSAEACHYVAQNCEANIIVVENQKQLQKILQ---IQDQLPHLKAIIQYKDELKE--KRPNLYTWKEFMQLGKDIPDSQLDQIISSQKPNQCCTLIYTSGTTGQPKGVMLSHDNITWTAASAGKTVRLREATDMQEIVVSYLPLSHIAAQMIDIWLTMKHGGATYFAQPDALKGSLANTLREVRPTAFMGVPRVWEKMQEKMKAVGAKSSTIKRKVATWAKGVGLETNLKKMNGSTPHPMKYHVAKKLVFKKVRKALGLDRCTK---CYTGAAPITKDTLEFFLSLNIPVYELYGMSESSGPHTISLPDA-FRITSCGKVISGCKTKIHQPDNDGSGEILFWGRHVFMGYLNMEDKTQESLDEEGWLHSGDIGKHDENGFLYITGRIKELIITAGGENIPPVPIEDALKEQVP-IISNAMVIGDKKKFLSMLLTLKCNVNADTGEPEDELTPEAIEFCRQIGSKATLVS--DIVGG--KDTAVYAAIQDGVNSVNQKATSNAQKVQKWLILDQDFSIAGGELGPTMKLKRPVVAKMYKDQIDSFYQD 646          

BLAST of amp dependent ligase vs. nr
Match: gi|542246312|ref|XP_003455309.2| (PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2 [Oreochromis niloticus] >gi|542246314|ref|XP_005459838.1| PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2 [Oreochromis niloticus])

HSP 1 Score: 442.58 bits (1137), Expect = 1.348e-142
Identity = 251/648 (38.73%), Postives = 384/648 (59.26%), Query Frame = 0

Query:   45 VKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSRV--TLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHH--LRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYS 652
            V++RMED+ PG+  PI +  M  E+VE++GD  AL +K  DG W + T+  YY   R+ A +F+ LGL+   GVGI+  NS EW IS +  I AGGL+ GIYTT+S +  QYVA N   ++LV+ENQ  LD +LQ   ++  LP ++ IV  + ++ Q  +  F+ +W++ ++LG                                     L HDNITWT+ +  T+ + +  +E +++YLPLSHVAA MVD ++      T+YFAD  ALKGSL + L+EVRPT FL VPRVWEKI+ KM+ A ++ S +KK ++ +AK  G +Y      G  +   G+ LA+ LV  +V  ALGLDR        +GAA +  DT +YF SL + +++L G +E+ GP T ++   + +  + GK  +G +  + + D++G GEI    RN+FMGY+N   +T +    +GW  +GDLGR DE+  L I GRIKELI+ +GG+NI P+PIE  +K  LP +ISN +++G+   FL+ L+T +  VD  + +PT+ L +    + +++ V  T       +  I +N    +   IQ G+E  NA ++S  +KVQK+ ++ ++FSV G ELGPT+KL+RP++ + Y + I+ MY+
Sbjct:  111 VRLRMEDSGPGSETPITIHQMFLETVEKYGDHPALVSK-KDGQWMTLTWNQYYEQCRAAAKSFLKLGLERYHGVGILGFNSAEWFISDIGCILAGGLAAGIYTTNSPEACQYVAANCEANVLVVENQKQLDKILQ---VKDHLPHLRAIVQYKGELQQ--KAPFLYTWEEFMKLGEDVPDEQLNAVIDSLRPNECCTLIYTSGTTGNPKGVMLSHDNITWTAHMGCTILKAKGGEEILVSYLPLSHVAAQMVDIWVCMGFAGTIYFADPDALKGSLVNTLKEVRPTCFLGVPRVWEKIEEKMKAAGAKASPMKKRVADFAKSIGLQYSYSVMNGKNQVPWGFMLANNLVFKKVRAALGLDRCKI---CCTGAAPITKDTLDYFMSLNIPVKELYGMSESCGPHTVSIN--EYRVTSCGKVMIGCKVKLVNTDADGNGEICFWGRNIFMGYLNMPDKTAEALDEDGWLHTGDLGRHDEQDFLYITGRIKELIITAGGENIPPVPIEDAVKSELP-IISNAMLIGDKLKFLSMLLTLKCVVD-DNGEPTDELNQEALEFCQQHGVKAT------KVSEITANKEPAIYSAIQAGIERVNARSTSNAQKVQKWDIVERDFSVTGGELGPTMKLRRPIVVKMYQEKINEMYA 739          

BLAST of amp dependent ligase vs. nr
Match: gi|1042357281|ref|XP_017322514.1| (PREDICTED: LOW QUALITY PROTEIN: long-chain-fatty-acid--CoA ligase ACSBG2 [Ictalurus punctatus])

HSP 1 Score: 441.81 bits (1135), Expect = 1.789e-142
Identity = 258/657 (39.27%), Postives = 375/657 (57.08%), Query Frame = 0

Query:   45 VKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGS-WKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSR---VTLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCI---QRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKR-----NNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYSE 653
            VK+RM ++ P +  P+ V  +   +VE+FGDR AL  K  DG  W+  T++DYY D R  A +F+ LGLQ   GVGI+  NSPEW IS + +I AGG + GIYTT+S     YVA N   +ILV+EN   L  +LQ   ++  LP +K IV  +  + +  +K  + SW++ +ELGR                                    L HDN+TWT+     T+   E  +E +++YLPLSH+AA MVD +++   G   YFA   ALKGSL + LREVRPT FL VPRVWEK+Q KM+   +++S V++ ++ WAK  G +  +   ++     +  L Y LA +LV  +V +ALGLDR V     ++GAA +  DT E+F SL + L +L G +E+TGP + +L     +  + GK   G +  I +PDS+  GEI    R++FMGY+N   +T +    +GW  SGDLG+ D++  L I GRIKELI+ +GG+NI P+PIE  +K+A+P +ISN +++G+ R FL+ L+T + QV+  +  P + L      W  +       V E       L+ A          IQ G+   N +A+S  ++VQKF VL Q+FS+ G ELGPT+KLKRPV+ + Y + I+N Y E
Sbjct:   92 VKLRMAESGPASAAPLTVNQLFTRTVEQFGDRTALGWK--DGEQWRQSTYRDYYRDCRIAAKSFLKLGLQRHHGVGILGFNSPEWFISDIGAILAGGFAVGIYTTNSPDACHYVAENCQANILVVENHKQLQKILQ---IQDKLPHLKAIVQYKDALKE--KKPNLYSWEEFMELGRDEPDAPLDDIIASQKXNQCCMLIYTSGTTGQPKGVMLSHDNLTWTAFSTVYTVNLSETSQETVVSYLPLSHIAAQMVDIWITMRVGGATYFAQPDALKGSLVNTLREVRPTAFLGVPRVWEKMQEKMKAVGAKSSTVRRKVASWAKDVGLQTNLSKMEQNGAMTRTPLNYRLAKRLVFRKVRKALGLDRCVH---CFTGAAPITKDTLEFFFSLDIPLYELYGMSESTGPHSMSLPD-ALRLTSCGKVIAGCQTKIFNPDSDENGEICFWGRHIFMGYLNMVDKTEEALDEDGWLHSGDLGKHDKDNFLYITGRIKELIITAGGENIPPVPIEDAVKEAVP-IISNAMLIGDKRKFLSMLLTLKCQVNSETGVPEDELTPEAVEWCSKLGSSSTRVSEICGGRDRLVHA---------AIQDGINRVNENATSNAQRVQKFTVLEQDFSLPGGELGPTMKLKRPVVMKMYKEQIENFYKE 727          

BLAST of amp dependent ligase vs. nr
Match: gi|499050331|ref|XP_004575689.1| (long-chain-fatty-acid--CoA ligase ACSBG2 isoform X1 [Maylandia zebra] >gi|836009637|ref|XP_012771619.1| long-chain-fatty-acid--CoA ligase ACSBG2 isoform X1 [Maylandia zebra])

HSP 1 Score: 441.81 bits (1135), Expect = 3.004e-142
Identity = 253/660 (38.33%), Postives = 389/660 (58.94%), Query Frame = 0

Query:   33 ATDFISWNKHVPVKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSRV--TLTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTNVLEESVTVWLKRNNVPETITTSHDLIEAIQSNHH--LRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYS 652
            A    S  +   V++RME++ PG+  PI V  M  E+VE++G+  AL +K  DG W + T+  YY   R+ A +F+ LGL+   GVGI+  NS EW IS +  I AGGL+ GIYTT+S +  QYVA N   ++LV+ENQ  LD +LQ   ++  LP ++ IV  + ++ Q  +  F+ +W++ ++LG                                     L HDNITWT+ V  T+ + +  +E +++YLPLSHVAA M+D ++      T+YFA+  ALKGSL + L+EVRPT FL VPRVWEKI+ KM+   ++ S +KK ++ +AK  G +Y      G  +   G+ LA+ LV  +V  ALGLDR        +GAA +  DT +YF SL + L++L G +E++GP T ++   + +  + GK  +G +  + +PD++G GEI    RN+FMGY+N   +T +    +GW  +GDLGR DE+  L I GRIKELI+ +GG+NI P+PIE  +K  LP +ISN +++G+   FL+ L+T +  VD  + +PT+ L +    + ++++V  T       +  I +N    +   IQ G+E  NA ++S  +KVQK+ ++ Q+FSV G ELGPT+KL+RP++ + Y + I+ MY+
Sbjct:  103 ADQLWSTGREQAVRLRMEESGPGSEAPITVHQMFLETVEKYGNHPALVSK-KDGQWMTLTWNQYYQQCRAAAKSFLKLGLERYHGVGILGFNSAEWFISDIGCILAGGLAAGIYTTNSPEACQYVAANCEANVLVVENQKQLDKILQ---VKDHLPHLRAIVQYKGELQQ--KAPFLYTWEEFMKLGEDVPDEQLNAVIDSLRPNECCTLIYTSGTTGNPKGVMLSHDNITWTAHVGCTILKAKGGEEILVSYLPLSHVAAQMIDIWVCMGFAGTIYFAEPDALKGSLVNTLKEVRPTCFLGVPRVWEKIEEKMKAVGAKASPMKKRVAGFAKSIGLQYNYSVMNGQNQVPWGFMLANNLVFKKVRAALGLDRCRI---CCTGAAPITKDTLDYFMSLNIPLKELYGMSESSGPHTISI--DEYRVTSCGKVMIGCKIKLVNPDADGNGEICFWGRNIFMGYLNMPDKTAEALDEDGWLHTGDLGRYDEQDFLYITGRIKELIITAGGENIPPVPIEDAVKSELP-IISNAMLIGDKLKFLSMLLTLKCVVD-DNGEPTDELNQEALEFCRQHSVKAT------KVSEIIANKEPAIYSAIQAGIERVNARSTSNAQKVQKWVIVEQDFSVTGGELGPTMKLRRPIVVKMYQEKINEMYA 743          

BLAST of amp dependent ligase vs. nr
Match: gi|1062780192|ref|XP_017944171.1| (PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2 isoform X5 [Manacus vitellinus] >gi|1062780195|ref|XP_017944172.1| PREDICTED: long-chain-fatty-acid--CoA ligase ACSBG2 isoform X5 [Manacus vitellinus])

HSP 1 Score: 439.113 bits (1128), Expect = 3.180e-142
Identity = 258/679 (38.00%), Postives = 382/679 (56.26%), Query Frame = 0

Query:   27 DPVDSKATDFIS---WNKHV--PVKIRMEDNYPGNLPPILVPTMMKESVERFGDRKALSTKLSDGSWKSWTFQDYYHDVRSVALAFVALGLQSKEGVGIMASNSPEWMISSVASIWAGGLSCGIYTTSSKQTVQYVANNTTVSILVLENQTFLDNLLQGRELRKVLPTVKKIVLVETDIIQPHQKSFVMSWKQLVELGR------------------------------------LHHDNITWTSRVT-----LTQYEWEKEDILNYLPLSHVAALMVDCYMSWLGGNTVYFADKLALKGSLPDYLREVRPTRFLAVPRVWEKIQAKMQDAASRNSGVKKSISQWAKKEGRRYCIQRRLGPVKPSLGYTLAHKLVLSRVHQALGLDRAVAKDGLYSGAATLPIDTFEYFQSLGMELQDLLGCTETTGPQTTNLAGPKCKPGTVGKPYLGVRNSIQDPDSNGVGEISTRSRNVFMGYINEELQTIQTFTPEGWFKSGDLGRLDEEGNLIICGRIKELILPSGGKNIAPIPIETRIKDALPELISNVVVVGEGRHFLACLITPRVQVDMVSNQPTN-----VLEESVTVWLKRNNVPETITTSHDLIEAIQSNHHLRQIIQGGLEMANAHASSRPEKVQKFRVLPQEFSVLGEELGPTLKLKRPVIHEKYAKVIDNMYSEI 654
            D VD KA+   +   W  H    VK+RMED   G+ PP  V  + +E+V ++GD  AL++K   G W   T++ YY +    A +F+ LGLQ   GVGI+  NS EW I+ + +I AGG + GIYTT+S +   YVA N +  ++V+ENQ  L  +L   E+   LP +K IV    +I +  ++  + SW + +ELGR                                    L HDN+TWT+ V      LT  +  +E +++YLPLSH+AA M D + +   G  VYFA   ALKGSL + LREVRPT FL VPRVWEK++ KM+    ++S +++ ++ WAK  G +  ++R  G  +  + + LA  LV  +V +ALGLDR       Y+GAA +  +T E+F SL + + +L G +E++GP T +L     K  + GK   G R  I  PD +G+GEI    R++FMGY+N E +T +    +GW  SGDLG+ D++G L I GRIKELI+ +GG+N+ P+PIE  +K+A+P +ISN ++VG+   FL+ L+T + +VD  + +P +      LE    +  K   V E I++    I A          IQ G+   N  A+S  +KVQK+ +L ++FS+ G ELGPT+KLKRPV+ +KY   I   Y ++
Sbjct:    7 DSVDPKASSSPASRVWTTHRDGEVKLRMEDQGIGSEPPKTVHQLFQETVNKYGDYYALASK-KGGQWVKLTYKMYYDECCKAAKSFLKLGLQRFHGVGILGFNSAEWFIADIGAILAGGFAVGIYTTNSPEACHYVAENCSADVIVVENQKQLQKIL---EIEDRLPQLKAIVQYGEEIKE--KRPNLYSWSEFMELGRDVPDTLLHLAIAAQKPNQCCTLIYTSGTTGQPKGVMLSHDNLTWTAAVAGRSIMLTDPKERQEQVISYLPLSHIAAQMSDIWSAMTFGVQVYFAQPDALKGSLVETLREVRPTAFLGVPRVWEKMEEKMKSVGMKSSALRRKVASWAKGVGLQTNLKRMNGYSEVPVNFRLAKHLVYRKVRKALGLDRCTK---CYTGAAPITRETLEFFLSLNIPVLELYGMSESSGPHTISLPHA-FKLTSCGKELSGCRTLIHKPDRDGIGEICFSGRHIFMGYLNMEDKTKEAIDEDGWLHSGDLGKHDKDGFLYITGRIKELIITAGGENVPPVPIEDAVKNAVP-IISNAMLVGDKAKFLSMLLTLKCKVDAETGEPGDELTPEALEYCQKLGSKATKVSEIISSKDKAIYA---------AIQKGITAVNEGATSNAQKVQKWVLLEKDFSLFGGELGPTMKLKRPVVAQKYKAQIAQFYGDV 665