BLAST of neutral sphingomyelinase vs. L. salmonis genes
Match: EMLSAG00000012239 (supercontig:LSalAtl2s:LSalAtl2s890:157027:158965:1 gene:EMLSAG00000012239 transcript:EMLSAT00000012239 description:"maker-LSalAtl2s890-augustus-gene-0.11")

HSP 1 Score: 217.238 bits (552), Expect = 1.301e-67
Identity = 140/360 (38.89%), Postives = 197/360 (54.72%), Query Frame = 0

Query:   52 DRRQLLDDEGSAR-SRSRVR-ALCHSWRWCVGIWIILACIILGHALSIYY-IYFRCNHSNCVGVKVLTLNTWGMPASLGSQFKTERMKAIAQELSRGDH-DIYLLQELWMNPDWNTLHAALPSGYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPG-MVVDVFVTHTAADPDPSHGYDNRYYRIRQVQELMDTYVTHSEADVVLLGGDFNAGPNQTAGEPYAMVKDHMQNCIEEVFYKLLEWLNPQYATYGNQKNTFSNHYT-PIIYDYIFHHTRDPNVVRVWTNLFELPFFKFFLKKDHEISFSDHEAVEATIYMWKK 405
            DR  LL+ +   R  R ++R  +C      + + IIL   I+G  L+ Y+  YF  +H+    +K+L+LN WG+P   GS  K ER+  IA+ L +    D+   QELWM  D  T+   L   Y MT F  +    C     P  CSGL ++SKYPF E EF++Y   G   + +ID E    KGVGRVR+  LPG    D F+THT AD  P   Y N   R+ QV EL++  V  S AD+V+LGGD NA P+    +PY  +K  M+    ++F  L    +P  +TY N KNT+S + T PI+YD+IF+   +P+ +  WTN+F +P F F      + S SDHE + AT+Y  +K
Sbjct:    4 DRFXLLNTDYPQRIPRHKLRHVMC-----ILFLAIILVLFIIG--LAXYFGSYFGSHHT----IKILSLNVWGLPYKFGSLDKEERILKIAEMLRKDPQFDVVFFQELWMPADHATIELELNGDYSMTSFDSMN-NDCMHLTAPFQCSGLAVLSKYPFHEVEFHAYNESGHSSEEFIDGENFVNKGVGRVRLL-LPGNTTADFFLTHTIADAPPGKSYTNEDIRLNQVSELVNNGVKKSTADIVILGGDLNASPDS---KPYQFIKRCMRXL--DIFKILKALFDPVISTYANPKNTWSVNITDPIMYDHIFYKVNNPDYL-AWTNIFSVPIFHFLDDSGKKKSVSDHEGIIATLYYRRK 344          

BLAST of neutral sphingomyelinase vs. L. salmonis genes
Match: EMLSAG00000006794 (supercontig:LSalAtl2s:LSalAtl2s380:480697:482683:1 gene:EMLSAG00000006794 transcript:EMLSAT00000006794 description:"maker-LSalAtl2s380-augustus-gene-5.11")

HSP 1 Score: 142.124 bits (357), Expect = 1.367e-39
Identity = 98/311 (31.51%), Postives = 145/311 (46.62%), Query Frame = 0

Query:   97 IYYIYFRCNHSNCVGVKVLTLNTWGMPASLGSQFKTERMKAIAQELSRG-DHDIYLLQELWMNPDWNTLHAALPSGYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPGMVVDVFVTHTAADPDPSHGYDNRYYRIRQVQELMDTYVTHSEADVVLLGGDFNAGPNQTAGEPYAMVKDHMQNCIEEVFYKLLEWLNPQYATYGNQKNTFS-NHYTPIIYDYIFHHTRDPNVVRVWTNLFELPFFKFFLKKDHEISFSDHEAVEATIYMWKK 405
            +Y+ Y+  +H     +K+L+LN WG+P + GS  K ER+  IA+ L +  + D+   +ELWM  D  T+   L   Y MT F   ++  C     P  CS                                                G+ V +      AD  P   Y N   R+ QV EL+   V  S ADVV+LGGDFNA P+    +PY  +K +M++   ++   L    +P  +TY N KNT+S N   PI+YD+IF+   + +++ VWTN+F +P F F      + SFSDHE + AT+Y  +K
Sbjct:   44 VYFGYYFGSHHT---LKILSLNVWGLPYAFGSLDKEERILKIAEMLHKDPEFDVVFFEELWMPADHATIELELEGVYIMTSFE--SMNPCMHLTAPLKCS------------------------------------------------GLAVLI------ADAPPGKNYTNDDIRLDQVSELVRNGVQRSTADVVILGGDFNASPD---SKPYEFIKRYMRSTALDILKILKALFDPVISTYANPKNTWSYNISDPIMYDHIFYKINNHDLL-VWTNIFSVPLFHFLDDSGKKKSFSDHEGITATLYYRRK 291          

BLAST of neutral sphingomyelinase vs. L. salmonis genes
Match: EMLSAG00000008458 (supercontig:LSalAtl2s:LSalAtl2s519:4376:14325:1 gene:EMLSAG00000008458 transcript:EMLSAT00000008458 description:"augustus_masked-LSalAtl2s519-processed-gene-0.0")

HSP 1 Score: 140.969 bits (354), Expect = 1.548e-38
Identity = 97/290 (33.45%), Postives = 154/290 (53.10%), Query Frame = 0

Query:  108 NCVGVKVLTLNTWGMPASLGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWNTLHAALPSGYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSW-IDAEWLARKGVGRVRIEPLPGMVVDVFVTHTAADPDPSHGYDNRYYRIRQVQELMDTYVTHSEADVVLLGGDFNAGPNQTAGEPYAMVKDHMQNCIEEVFYKLLEWLNPQYATYGNQKNTFSNHYTPIIYDYIFHHTRDPNVVRVWTNLFELPFFKFFLKKDHEISFSDHEAV 396
            N   +KVL LNTWG+     S+ +  R  A+  E+  G++DI LLQE+W+  D++ L  ALP   Y+T ++ L  G C G   P  CSGLTI+SK+P  + +   + + G     W  D E    KGV + RI    G  VDVF TH  +  +  +  DN  +R  Q  E +  ++ +S+AD+ L GGD N  P   + EPY ++   + + + + F +     +P Y+T+GN+KNT++  + P   DY+  +   P  +++ T  + +P F       ++IS SDHE +
Sbjct:   25 NASYLKVLNLNTWGLSWPW-SKDREHRFIALRDEIVNGNYDIVLLQEVWLRKDYDVLRPALP---YVTYYQTLN-GNCFGTFLPLGCSGLTILSKHPMQDAKMYPFQFKG---TFWNFDGEVFVGKGVAKARIL-WNGFTVDVFTTHLISYTNHPNQ-DNAKFRYMQALETV-RHIRNSDADIKLFGGDLNTLPITDSREPYGILSTVLTDSLIDKFPR--ASFSPYYSTFGNKKNTYTRDFVPERIDYLM-YANSPK-IKMKTFDYHVPNFYTVSSHGYKISLSDHEPI 299          

BLAST of neutral sphingomyelinase vs. L. salmonis genes
Match: EMLSAG00000002075 (supercontig:LSalAtl2s:LSalAtl2s1389:10797:15464:-1 gene:EMLSAG00000002075 transcript:EMLSAT00000002075 description:"maker-LSalAtl2s1389-augustus-gene-0.10")

HSP 1 Score: 109.383 bits (272), Expect = 6.291e-27
Identity = 96/334 (28.74%), Postives = 157/334 (47.01%), Query Frame = 0

Query:   83 WIILACIILGHALSIYYIYFRCNH-----SNCVGVKVLTLNTWGMPASLGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWNTLHAALPSGYYMTGFRDLA-LGTCDGRVGPEFCSGLTIVSKYPFLEK---EFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPGMVVDVFVTHTAADPDPSHGYDNR-YYRIRQVQELMDTYVTHSEADVVLLGGDFNAGPNQTAGEPYAMVKD---HMQNCIEEVFYKLLEWLNPQ-YATYGNQKNTFS-NHYTPIIYDYIFHHTR---DPNVVRVWTNLFE--LPFFKFFLKKDHEISFSDHEAV 396
            +++L   +L    S  ++    N+     +  + +K+ +LN W +P   G+  K ER+K +   L     DI++LQ LWM  D  ++  ALPS   MT FR+L     CDGR+    CSGL+IV K     K    F+ YTY G    S    +    KG+GR+R++ +   +V++F TH          +D+  YY   Q +ELM +Y + S AD+++LG    + P +  G    M  D    + N  ++ F     W +P  + T+   +N F+     P I D+ F  +      +V++ +  +FE   PF          +S S+HE +
Sbjct:   61 YVLLTIGLLFAIFSTVFVLLNQNNHIRPVTKSIQLKIYSLNNWAIP---GNPDKEERLKELRTVLRNESFDIFILQGLWMEYDHTSIREALPSDKSMTDFRELXNSNICDGRITRGGCSGLSIVYKNNGTTKRSSSFDLYTYRG----SIFTTDGTLGKGIGRLRLK-IQDFIVEIFTTHV---------HDSEVYYXDLQTRELM-SYXSKSPADLIILGAAIQSTPXE-RGYRNIMTYDATAKISNTAQKYFPS--NWNDPNAHGTFNMPQNKFTPKGNVPKITDFAFAASALDYSVSVIQFYNPIFEKKYPF---------NVSLSNHEVI 364          

BLAST of neutral sphingomyelinase vs. L. salmonis genes
Match: EMLSAG00000000343 (supercontig:LSalAtl2s:LSalAtl2s104:671412:673075:-1 gene:EMLSAG00000000343 transcript:EMLSAT00000000343 description:"maker-LSalAtl2s104-snap-gene-6.30")

HSP 1 Score: 72.0182 bits (175), Expect = 4.360e-14
Identity = 81/295 (27.46%), Postives = 132/295 (44.75%), Query Frame = 0

Query:  133 ERMKAIAQELS-RGDHDIYLLQELWMNPDWNTLHAALP--------SGYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPGMVVDVFVTHTAADPDPSHGYDNRYYRIRQVQELMDTYVTHSEADVVLLGGDFNAGPNQTAGEPYAMVK--DHMQNCIEEVFYKLLEWLNPQYATYGNQKNTFSNHYTP----------IIYDYIFHHT-RDPNVVRVWTNLFELPFFKFFLKKDHEISFSDHEAVEATIYMWKK 405
            ER++A++  LS + D+DI LLQE+W + D+ TL   L         S Y+  G   +  GTC             I SK       F+S+  +G P +S    +W A KG+G + +  + G  + V+V+H  A+ +P +     +  +  ++      +T + AD+ +  GDFN  P++    P  ++K    +++  EEV               G    T+ N +TP             DYI +    D +VV   T    LP  K    K++  S+SDHEAVE+T  + +K
Sbjct:   15 ERVQALSHYLSAKEDYDIVLLQEVWTDEDYKTLKKNLILSGGPFKFSHYFDNGI--IGTGTC-------------IFSKSRIRFTTFHSFGLNGYP-QSISHGDWFAAKGLG-IAVLYIDGFEIHVYVSHYHAEYNPENDIYLGHRVVHALESAQWIQLTSASADLTIYAGDFNTEPDKV---PLKLLKYITSLKDSWEEVH-------GSNANEEGATCETYYNSFTPESVKQACPEGKRIDYIMYQAGADTDVV---TKDCILPLSKRVPGKNY--SYSDHEAVESTFRIRRK 277          

BLAST of neutral sphingomyelinase vs. SwissProt
Match: gi|20138888|sp|O74369.2|CSS1_SCHPO (RecName: Full=Inositol phosphosphingolipids phospholipase C; Short=IPS phospholipase C; Short=IPS-PLC)

HSP 1 Score: 86.6557 bits (213), Expect = 2.073e-17
Identity = 60/212 (28.30%), Postives = 97/212 (45.75%), Query Frame = 0

Query:  112 VKVLTLNTWGMPASLGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWNTLHAALPSGY-YMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPGMVVDVFVTH------TAADPDPSHGYDNRYYRIRQVQELMDTYVTHSEADVVLLGGDFNAGPNQTAGE---PYAMVKD 313
            ++VL+ N WG+     S+++TER+KA+ ++L++ D+DI LLQE+W   D+  +   +     Y   F   A+G           +GL + SK+P +E   N Y  +G P   W   +W   KGV    ++   G ++ +F TH        AD    H     +Y    + +L+   V      +V+  GDFN  P     E    Y +V D
Sbjct:    9 LRVLSFNCWGL--RFVSKYRTERLKAVGEKLAKCDYDIVLLQEVWSIYDFQEIRNLVSCNLVYSRFFHSAAMG-----------AGLAMFSKFPIIESSMNKYPLNGRPQAFW-RGDWYVGKGVATASLQHPSGRIISLFNTHLHAPYGKGADTYLCHRLSQAWY----ISKLLRAAV--QRGHIVIAAGDFNIQPLSVPHEIITSYGLVND 200          

BLAST of neutral sphingomyelinase vs. SwissProt
Match: gi|32172461|sp|Q9VZS6.2|NSMA_DROME (RecName: Full=Putative neutral sphingomyelinase)

HSP 1 Score: 85.5001 bits (210), Expect = 5.741e-17
Identity = 82/295 (27.80%), Postives = 129/295 (43.73%), Query Frame = 0

Query:  118 NTWGMPASLGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWNTLH----AALPSGYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPGMVVDVFVTHTAADPDPSHGYDNRYYRIRQVQELMDTY----VTHSEADVVLLGGDFNAGPNQTAGEPYAMVKDHMQNCIEEVFYKLLEWLNPQYATYGNQKNTFSNHYTPIIYDYIFHHTRDPNVVRVWTNLFELPFFKFFLKKDHEISFSDHEAVEATIYMWK 404
            N WG+P    S  +  R+ AI +EL+ G +DI  LQE+W   D   L     A LP  +Y   F    +G           +GL ++SKYP L   F++++ +G        A+W   KGVG  RI  + G +V ++  H  A+ D +    N  Y+  +V +  DT      T   + + +L GD NA P   + +        + +C  + F       N   +    ++N        I  D+IF   R  + V      ++LPF +       + SFSDHEAV A + ++K
Sbjct:   12 NIWGIPYV--SSDRRPRIDAICKELASGKYDIVSLQEVWAQEDSELLQKGTEAVLPHSHY---FHSGVMG-----------AGLLVLSKYPILGTLFHAWSVNG-YFHRIQHADWFGGKGVGLCRI-LVGGQMVHLYNAHLHAEYDNA----NDEYKTHRVIQAFDTAQFIEATRGNSALQILAGDLNAQPQDISYKVLLYTSKMLDSCDSDSFRTNECEHNSYTSKQARERNPLG-----IRIDHIF--VRGGDHVNAEIAEYKLPFPERV--PGEKFSFSDHEAVMAKLKLFK 275          

BLAST of neutral sphingomyelinase vs. SwissProt
Match: gi|160332206|sp|O60906.2|NSMA_HUMAN (RecName: Full=Sphingomyelin phosphodiesterase 2; AltName: Full=Lyso-platelet-activating factor-phospholipase C; Short=Lyso-PAF-PLC; AltName: Full=Neutral sphingomyelinase; Short=N-SMase; Short=nSMase)

HSP 1 Score: 73.1738 bits (178), Expect = 6.622e-13
Identity = 60/196 (30.61%), Postives = 94/196 (47.96%), Query Frame = 0

Query:  112 VKVLTLNTWGMPASLGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWNTLHAALPSGYYMT-GFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPGMVVDVFVTHTAADPDPSHGYDNR-----YYRIRQVQELMD-TYVTHSEADVVLLGGDFNAGP 300
            +++  LN WG+P    S+ + +RM+ +   L++   D+ LL+E+W   D+  L   L   Y     FR   +G           SGL + SK+P  E   + YT +G P       +W + K VG + +  L GMV++ +VTH  A+      Y+ +      +R+ Q  EL    + T  +ADVVLL GD N  P
Sbjct:    9 LRIFNLNCWGIPYL--SKHRADRMRRLGDFLNQESFDLALLEEVWSEQDFQYLRQKLSPTYPAAHHFRSGIIG-----------SGLCVFSKHPIQELTQHIYTLNGYPYMIH-HGDWFSGKAVGLLVLH-LSGMVLNAYVTHLHAE------YNRQKDIYLAHRVAQAWELAQFIHHTSKKADVVLLCGDLNMHP 183          

BLAST of neutral sphingomyelinase vs. SwissProt
Match: gi|20138814|sp|Q9ET64.1|NSMA_RAT (RecName: Full=Sphingomyelin phosphodiesterase 2; AltName: Full=Lyso-platelet-activating factor-phospholipase C; Short=Lyso-PAF-PLC; AltName: Full=Neutral sphingomyelinase; Short=N-SMase; Short=nSMase)

HSP 1 Score: 72.7886 bits (177), Expect = 8.050e-13
Identity = 62/203 (30.54%), Postives = 98/203 (48.28%), Query Frame = 0

Query:  105 NHSNCVGVKVLTLNTWGMPASLGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWNTLHAAL----PSGYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPGMVVDVFVTHTAADPDPSHGYDNRY-YRIRQVQELMD-TYVTHSEADVVLLGGDFNAGPN 301
             H+  + ++V  LN W +P    S+ + +RMK +   L+    D+ LL+E+W   D+  L   L    P  +Y   FR   +G           SGL + S++P  E   + YT +G P K +   +W   K VG + +  L G+V++ +VTH  A  + S   D  + +R+ Q  EL    + T  +A+VVLL GD N  P 
Sbjct:    2 KHNFSLRLRVFNLNCWDIPYL--SKHRADRMKRLGDFLNLESFDLALLEEVWSEQDFQYLKQKLSLTYPDAHY---FRSGIIG-----------SGLCVFSRHPIQEIVQHVYTLNGYPYK-FYHGDWFCGKAVGLLVLH-LSGLVLNAYVTHLHA--EYSRQKDIYFAHRVAQAWELAQFIHHTSKKANVVLLCGDLNMHPK 184          

BLAST of neutral sphingomyelinase vs. SwissProt
Match: gi|20138887|sp|O70572.1|NSMA_MOUSE (RecName: Full=Sphingomyelin phosphodiesterase 2; AltName: Full=Lyso-platelet-activating factor-phospholipase C; Short=Lyso-PAF-PLC; AltName: Full=Neutral sphingomyelinase; Short=N-SMase; Short=nSMase)

HSP 1 Score: 70.0922 bits (170), Expect = 7.336e-12
Identity = 61/196 (31.12%), Postives = 94/196 (47.96%), Query Frame = 0

Query:  112 VKVLTLNTWGMPASLGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWNTLHAAL----PSGYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPGMVVDVFVTHTAADPDPSHGYDNRY-YRIRQVQELMD-TYVTHSEADVVLLGGDFNAGPN 301
            ++V  LN W +P    S+ + +RMK +   L+  + D+ LL+E+W   D+  L   L    P  +Y   FR   +G           SGL + SK+P  E   + Y+ +G P   +   +W   K VG + +  L G+V++ +VTH  A  + S   D  + +R+ Q  EL    + T   ADVVLL GD N  P 
Sbjct:    9 LRVFNLNCWDIPYL--SKHRADRMKRLGDFLNLENFDLALLEEVWSEQDFQYLRQRLSLTYPDAHY---FRSGMIG-----------SGLCVFSKHPIQEIFQHVYSLNGYPYM-FHHGDWFCGKSVGLLVLR-LSGLVLNAYVTHLHA--EYSRQKDIYFAHRVAQAWELAQFIHHTSKNADVVLLCGDLNMHPK 184          

BLAST of neutral sphingomyelinase vs. SwissProt
Match: gi|33302616|sp|O45870.2|NSMA_CAEEL (RecName: Full=Putative neutral sphingomyelinase)

HSP 1 Score: 60.4622 bits (145), Expect = 8.017e-9
Identity = 57/212 (26.89%), Postives = 91/212 (42.92%), Query Frame = 0

Query:   99 YIYFRCNHSNCVGVKVLTLNTWGMPAS--LGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWNTLHAALPS-----GYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPGMVVDVFVTHTAADPDPSHGYDNRYYRIRQVQEL-MDTYVTHSE--ADVVLLGGDFNAGP 300
            +   R N+     ++V+TLN W +P    +GS  +  R+  I Q +    +DI  LQELW   D+  L   + S      Y+ +GF                 SG+ + S++P +    N Y+ +G         +W   K VG   IE    + V+ + TH  A+ D     ++ Y   R  Q   +  +V H+   ADVV++ GD N  P
Sbjct:   26 FAALRRNNVAARQLRVVTLNAWCLPQPWPIGSTDRVHRLNKIGQYMIDELYDIVGLQELWSYYDFVRLSEQVSSVYPYFHYFHSGFTG---------------SGVCVFSRHPIVSTLTNRYSLNGFA-HHIHRGDWFGGKVVGLTEIEIDGDLRVNFYTTHLHAEYDRE---NDLYLPHRTAQAFELAQFVRHTARGADVVIVTGDLNMEP 218          

BLAST of neutral sphingomyelinase vs. nr
Match: gi|566019844|gb|ETI42755.1| (hypothetical protein F443_12181 [Phytophthora parasitica P1569] >gi|567985826|gb|ETL36162.1| hypothetical protein L916_11829 [Phytophthora parasitica] >gi|568015234|gb|ETL89411.1| hypothetical protein L917_11671 [Phytophthora parasitica] >gi|568045512|gb|ETM42665.1| hypothetical protein L914_11734 [Phytophthora parasitica] >gi|970631406|gb|KUF78017.1| hypothetical protein AM587_10008712 [Phytophthora nicotianae])

HSP 1 Score: 97.4413 bits (241), Expect = 2.695e-18
Identity = 88/307 (28.66%), Postives = 141/307 (45.93%), Query Frame = 0

Query:  110 VGVKVLTLNTWGMPASLGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWN-TLHAALPSGYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPGMVVDVFVTHTAAD------PDPSHGYDNRYYRIRQVQELMDTYVTHSEADVVLLGGDFNAGPNQTAGEPYAMVKDHMQNCIEEVFYKLLEWLNPQYA--------TYGNQKNTFSNHYTPIIYDYIFHHT-RDPNVVRVWTNLFELPFFKFFL--KKDHEISFSDHEAVEA 398
            V ++VL+LN WG+P +      TER   IA  +S  D D+ +LQE+W   + N  +  A  SG++   + + A+G         F +GL I+SK+P     ++S++  G P  +  +++++A KGVG +R+E  P   +DV+VTH  A+      P P   Y  R +R  Q  EL       +   + ++ GDFN+ P                +C+E    K L  L   Y         T+ +  N +S+   P+  DY+ +   + P     W +L E   FK F    K  +   SDH  V+A
Sbjct:   44 VKLRVLSLNAWGLPVAPKC---TERAAEIASAIS-SDFDLVVLQEIWHIRERNLVISKAQKSGFHYYHYFNPAVGFPFPMGADSFGTGLLILSKFPISNALYHSFSLSGRPY-ALHESDFVANKGVGLLRVE-TPAGQIDVYVTHLLANYNVGGKPGPGDFY--RGHRTGQSYELTQFISATNRNSLTIVCGDFNSSP----------------DCLELKIPKQLLSLRDAYTDTNDEDGLTFASPDNKYSHGEHPMRMDYVMYKIDKRPKGQSDW-HLVESSIFKGFFTDAKGEKTPLSDHFGVKA 325          

BLAST of neutral sphingomyelinase vs. nr
Match: gi|1059204733|ref|XP_017757020.1| (PREDICTED: putative neutral sphingomyelinase, partial [Eufriesea mexicana])

HSP 1 Score: 96.6709 bits (239), Expect = 2.867e-18
Identity = 92/312 (29.49%), Postives = 135/312 (43.27%), Query Frame = 0

Query:  110 VGVKVLTLNTWGMPASLGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWNTLHAA----LPSGYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWI----DAEWLARKGVGRVRIEPLPGMVVDVFVTHTAADPDPSHGYDNRYYRIRQVQELMDTY----VTHSEADVVLLGGDFNAGPNQTAGEPYAMVKDHMQNCIEEVFYKLLEWLNPQYATYGNQKNTFSNHYTPIIY----------DYIFHHTRDPNVVRVWTNLFELPFFKFFLKKDHEISFSDHEAVEAT 399
            V + +LTLN WG+P    SQ ++ RM AIA++L+  ++DI  LQE+W   D+  + A     LP  +Y             G VG    SG+ I+S++P          YH  PL  +I     A+W   KGVG  R++ +  M ++V++ H  A+ D      N  Y   +V +  DT     +T+  AD V+LGGD N  P   A      V      C                ++     N  +N YT              DYI +  ++   +++    F  PF K    KD   S+SDHEAV AT
Sbjct:   21 VSINILTLNCWGIPYI--SQNRSARMTAIAEKLATENYDIICLQEVWSINDFKMIRAKTQEQLPYSHYFY----------SGVVG----SGICILSRFP-----IKDVIYHKWPLNGYIHKIHHADWFGGKGVGLCRLQ-IHNMNINVYIVHLHAEYDR----HNDEYIAHRVLQAFDTAQFIRMTYGGADSVILGGDLNTEPQDLAYRIICGVAGLTDAC-------------SNSSSNLGTNNCANNSYTSSKLARTLPEGKRIDYILY--QNAKNIKIEVTDFRHPFPKRIPYKD--FSYSDHEAVMAT 289          

BLAST of neutral sphingomyelinase vs. nr
Match: gi|1133426353|ref|XP_019868993.1| (PREDICTED: putative neutral sphingomyelinase [Aethina tumida])

HSP 1 Score: 96.2857 bits (238), Expect = 3.007e-18
Identity = 89/303 (29.37%), Postives = 142/303 (46.86%), Query Frame = 0

Query:  112 VKVLTLNTWGMPASLGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWNTLH----AALPSGYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVG--RVRIEPLPGMVVDVFVTHTAADPDPSHGYDNRYYRIRQVQELMDTY----VTHSEADVVLLGGDFNAGPNQTAGEPYAMVKDHMQNC-----IEEVFYKLLEWLNPQYATYGNQKNTFSNHYTPIIYDYIFHHTRDPNVVRVWTNLFELPFFKFFLKKDHEISFSDHEAVEAT 399
            +KV TLN WG+     S+ + ER++AIA+EL+ G +DI  LQE+W+  D+N +       LP  +Y             G  G    SG+ I+SKYP  E  F+ +  +G  +      +W   KGVG  ++RIE     +V+V+  H  A+ + +    +  Y + ++ +  +T      T + AD+V+L GD N  PN  A      V     +      + +      E L+  Y +    K+  S +   I  DYI +HT      +V  + ++LP  KF    + + S+SDHEAV  T
Sbjct:    3 LKVFTLNCWGIKYV--SKDREERIRAIAEELATGKYDIVCLQEVWLTCDYNLIKDKSSGVLPYAHYFYS----------GVTG----SGVCILSKYPIEEVFFHKWAING-YIHKIHHGDWFGGKGVGLCQLRIE---NFIVNVYSAHLHAEYNRA----SDEYLVHRILQAFNTAQFIKQTSAHADLVILAGDLNTEPNDLAYRIITSVGGLTDSFGEAGPVPDSMVATNEALDNSYTS----KSRISKNIPGIRIDYIMYHTGKNACAKV--DKYQLPLPKFV--PNTQYSYSDHEAVCVT 273          

BLAST of neutral sphingomyelinase vs. nr
Match: gi|570323401|gb|ETO71349.1| (hypothetical protein F444_12280 [Phytophthora parasitica P1976] >gi|570979686|gb|ETP40591.1| hypothetical protein F442_12094 [Phytophthora parasitica P10297])

HSP 1 Score: 97.0561 bits (240), Expect = 3.070e-18
Identity = 88/307 (28.66%), Postives = 141/307 (45.93%), Query Frame = 0

Query:  110 VGVKVLTLNTWGMPASLGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWN-TLHAALPSGYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPGMVVDVFVTHTAAD------PDPSHGYDNRYYRIRQVQELMDTYVTHSEADVVLLGGDFNAGPNQTAGEPYAMVKDHMQNCIEEVFYKLLEWLNPQYA--------TYGNQKNTFSNHYTPIIYDYIFHHT-RDPNVVRVWTNLFELPFFKFFL--KKDHEISFSDHEAVEA 398
            V ++VL+LN WG+P +      TER   IA  +S  D D+ +LQE+W   + N  +  A  SG++   + + A+G         F +GL I+SK+P     ++S++  G P  +  +++++A KGVG +R+E  P   +DV+VTH  A+      P P   Y  R +R  Q  EL       +   + ++ GDFN+ P                +C+E    K L  L   Y         T+ +  N +S+   P+  DY+ +   + P     W +L E   FK F    K  +   SDH  V+A
Sbjct:   44 VKLRVLSLNAWGLPVAPKC---TERAAEIASAIS-SDFDLVVLQEIWHIRERNLVISKAQKSGFHYYHYFNPAVGFPFPMGADSFGTGLLILSKFPISNALYHSFSLSGRPY-ALHESDFVANKGVGLLRVE-TPAGQIDVYVTHLLANYNVGGKPGPGDFY--RGHRTGQSYELTQFISATNRNSLTIVCGDFNSSP----------------DCLELKIPKQLLSLRDAYTDTNDEDGLTFASPDNKYSHGEHPMRMDYVMYKIDKRPKGQSDW-HLVESSIFKGFFTDAKGEKTPLSDHFGVKA 325          

BLAST of neutral sphingomyelinase vs. nr
Match: gi|675199143|ref|XP_008908456.1| (hypothetical protein PPTG_13802 [Phytophthora parasitica INRA-310] >gi|568091130|gb|ETN05954.1| hypothetical protein PPTG_13802 [Phytophthora parasitica INRA-310])

HSP 1 Score: 97.0561 bits (240), Expect = 3.339e-18
Identity = 88/307 (28.66%), Postives = 141/307 (45.93%), Query Frame = 0

Query:  110 VGVKVLTLNTWGMPASLGSQFKTERMKAIAQELSRGDHDIYLLQELWMNPDWN-TLHAALPSGYYMTGFRDLALGTCDGRVGPEFCSGLTIVSKYPFLEKEFNSYTYHGDPLKSWIDAEWLARKGVGRVRIEPLPGMVVDVFVTHTAAD------PDPSHGYDNRYYRIRQVQELMDTYVTHSEADVVLLGGDFNAGPNQTAGEPYAMVKDHMQNCIEEVFYKLLEWLNPQYA--------TYGNQKNTFSNHYTPIIYDYIFHHT-RDPNVVRVWTNLFELPFFKFFL--KKDHEISFSDHEAVEA 398
            V ++VL+LN WG+P +      TER   IA  +S  D D+ +LQE+W   + N  +  A  SG++   + + A+G         F +GL I+SK+P     ++S++  G P  +  +++++A KGVG +R+E  P   +DV+VTH  A+      P P   Y  R +R  Q  EL       +   + ++ GDFN+ P                +C+E    K L  L   Y         T+ +  N +S+   P+  DY+ +   + P     W +L E   FK F    K  +   SDH  V+A
Sbjct:   44 VKLRVLSLNAWGLPVAPKC---TERAAEIASAIS-SDFDLVVLQEIWHIRERNLVISKAQKSGFHYYHYFNPAVGFPFPMGADSFGTGLLILSKFPISNALYHSFSLSGRPY-ALHESDFVANKGVGLLRVE-TPAGQIDVYVTHLLANYNVGGKPGPGDFY--RGHRTGQSYELTQFISATNRNSLTIVCGDFNSSP----------------DCLELKIPKQLLSLRDAYTDTNDEDGLTFASPDNKYSHGEHPMRMDYVMYKIDKRPKGQSDW-HLVESSIFKGFFTDAKGEKTPLSDHFGVKA 325