BLAST of nephrin- partial vs. L. salmonis genes
Match: EMLSAG00000000795 (supercontig:LSalAtl2s:LSalAtl2s1133:22645:64219:1 gene:EMLSAG00000000795 transcript:EMLSAT00000000795 description:"maker-LSalAtl2s1133-augustus-gene-0.5")

HSP 1 Score: 121.709 bits (304), Expect = 2.054e-28
Identity = 109/459 (23.75%), Postives = 196/459 (42.70%), Query Frame = 0

Query:  158 PPVMAVIINGDVVDAKMIEVGHMVTLICKAQSGHPEPELAFYRNSEQMGTARKMR---------------NKFTFRASQRDNNAVFTCTAYNGF--SLMSRSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAKIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGD-VTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMNEAIGVPV--TQTIRLDLTSNERFIEA 596
            PP    I   D     +I     V L C      P+  + +YR + +  T  ++                +    R +Q+DN+  + C A +    +   R+ + L+V+ PPG  EI G        + + VT+ C+S   NP+  + +      Y N    D S      E       SRN ++             ++ T      +  +  C   NE S+  M   + ++ +F P+++ + GP + +  +SV  +C +  + P A + W ++    S    S    Q  P+  G  +TV ++++K ++             +  V C   +  L  D+   + ++ V++PPG P+I GL   + L  G  + L C S  AG+PP  ++WY G +++ S   +    A A + F+P  + N  +LRCEA+N A+  PV  +Q + +D + N   IE+
Sbjct:  126 PPKRIEITGLDSGSRIIIRENEEVELKCVVFEAKPKATIIWYRENSKFFTESRIDEDTIGEDAEHRVTSISSIRIRPTQKDNHVTWACEAVHPALKNAPMRASVLLSVQHPPGRPEITGYIEGETIRVAQSVTLICTSRGGNPLADVIW------YRNGELADKSYTSSGRE-------SRNTFT------------FIAETD----DDNARFRCEASNEFSAEPMDAEIVLSVQFAPSKIYLYGPNEARRDDSVDXSCSTXNSNPPAIIQWIID---NSXLDASSYFTQKLPSPMGGWITVSNISVKXEE----------NKKNKIVSCHVLNSELN-DVKTKSQILSVIYPPGTPTIQGLSFDDLLIAGKLKRLKCTSM-AGNPPPTLRWYRGKELLHSRTVITDQFASAELEFIPARKDNLGDLRCEAINSALRFPVYASQNLSVDFSPNFVKIES 540          

BLAST of nephrin- partial vs. L. salmonis genes
Match: EMLSAG00000004534 (supercontig:LSalAtl2s:LSalAtl2s237:371452:419819:1 gene:EMLSAG00000004534 transcript:EMLSAT00000004534 description:"maker-LSalAtl2s237-augustus-gene-4.26")

HSP 1 Score: 99.3673 bits (246), Expect = 1.568e-21
Identity = 67/247 (27.13%), Postives = 119/247 (48.18%), Query Frame = 0

Query:  349 NGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAKIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDT-----ALAHITFVPQLEYNGSELRCEAMNEAIGV---PVTQTIRLDL 587
            N  +  C  +N VS   +   + ++ +F P++V++ GP +    +++ F C ++ + P A + W V+      ++ + EN   R  +E          +++  +L  P       +  + C+A +  LGE ++   H + VL+PPG PSI+G+  G  ++ G  R LTC S  AG+P A + WY G ++++   Q D +T     A + +        NG   RCEA N A      P++ TI L +
Sbjct:   53 NNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEANVGDTLSFDCITTNSNPPAIVQWVVD------SRSAQENFTVRTESERG----GWETRSNISVLIGPNDR----NKIISCYAINNDLGETVVQ-THTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISM-AGNPLATLTWYRGDEIIQGTTQDDDETETKNYAQSELHLTANYSDNGMVYRCEASNPATAQESGPLSSTITLSV 283          

HSP 2 Score: 93.9745 bits (232), Expect = 7.175e-20
Identity = 73/251 (29.08%), Postives = 115/251 (45.82%), Query Frame = 0

Query:  177 VGHMVTLICKAQSGHPEPELAFYRNSEQMG-----TARKMRNKFTFRASQRDNNAVFTCTAYNGFSLMS-RSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSV----EQGGWITSANI--LLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTG--PAKI-KASESVVFTCQSSPAQPAARLAW 412
            +G  V+LIC +  G+P  E+ +YR  EQ+      T R+ RN +TF AS  DNN  + C A N  S+    +D+ L+V+F P  V I+GP      N+ + ++  C +  SNP   + +                        +V S+S++  ++V    E+GGW T +NI  L+      K+     I C   N      ++ + +I+  +PP + S+TG  P  I    +S   TC S    P A L W
Sbjct:    1 MGQTVSLICVSHGGNPLAEVVWYRGDEQIDFSYYTTGRESRNMYTFVASSEDNNKRYRCKAKNTVSVEPLTADIVLSVQFAPDKVTIEGPTEA---NVGDTLSFDCITTNSNPPAIVQW------------------------VVDSRSAQENFTVRTESERGGWETRSNISVLIGPNDRNKI-----ISCYAINNDLGETVVQTHTISVLYPPGKPSITGIDPGTIMDEGQSRRLTCISMAGNPLATLTW 219          

BLAST of nephrin- partial vs. L. salmonis genes
Match: EMLSAG00000008056 (supercontig:LSalAtl2s:LSalAtl2s479:94211:96038:-1 gene:EMLSAG00000008056 transcript:EMLSAT00000008056 description:"maker-LSalAtl2s479-snap-gene-1.12")

HSP 1 Score: 61.6178 bits (148), Expect = 5.292e-12
Identity = 27/63 (42.86%), Postives = 37/63 (58.73%), Query Frame = 0

Query:   37 VGGCNPDDLAEIGNGRKIVVRRFRGSVVRYRCLGGYTIYGQSRAHCLG-SEWSQTHPPICARI 98
            + GC+   L EI NG +  ++ +RGSVVRY C  GY +YG+ R HC     W+   PPIC  +
Sbjct:   26 LSGCDIATLPEIENGWRRPMKLYRGSVVRYTCAKGYKLYGRKRVHCDSYGHWNLDRPPICVPV 88          

BLAST of nephrin- partial vs. L. salmonis genes
Match: EMLSAG00000004704 (supercontig:LSalAtl2s:LSalAtl2s247:383895:431889:-1 gene:EMLSAG00000004704 transcript:EMLSAT00000004704 description:"maker-LSalAtl2s247-augustus-gene-3.26")

HSP 1 Score: 62.003 bits (149), Expect = 6.565e-10
Identity = 104/452 (23.01%), Postives = 174/452 (38.50%), Query Frame = 0

Query:  173 KMIEVGHMVTLICKAQSGHPEPELAFYRNSEQMGTAR--------------KMRNKFTFRASQRDNNAVFTCTAYNGFSLMSRSDL-------TLNVKFPPGNVEIQGPKRLILDNLK------EDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAKIKASESVV-FTCQSSPAQPAARLAWRV---EMGGKSV---TKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDTALAH--ITFVPQLEYNGSELRCEAMNEA-IGVPVTQTIRLDL 587
            K +  G+   + C + S +P  +L +Y+N E +  A                  ++   R  + DN     C A    ++ SRSD         LNV +PP     +GP  +IL +L+      E +T++C S   NP+  +++                G + ++                           +S  QLK + +  K     + E +S++++  ++  RE   N +S +       S S++ F C +    PA R+ W +   +  G S+   TK       Y  T++  ++V  L   A+++ L     +A+S     E          D I+    + VL PP  P ISGL   + L  G   +L C SSP G P  ++ WY+G         V     + H  I        N    RC A N+A +  P + TI L +
Sbjct:   91 KSVVAGNQEIIACTSSSSNPATKLLWYKNEELIQNAELEEEILLGDRPGTYTTVSRIKIRLVKEDNGVFIICVAXQP-AIFSRSDREYFQYKSKLNVLYPPE----RGP--IILGDLRPMRTKGEIITLTCLSRGGNPLPDLYWE--------------RGPRKINP--------------------------ISMPQLKSIPSTDK----NKKEGTSQSILKFVATPRE---NNISFS------CSRSLLNFNCTAFNGNPAPRIEWSLNGDDYVGXSINNHTKLDNRKGGYIFTSQ--ISVHILETDAEEISLAC---SAISRSYEGEIMKEI-----DEIS----ITVLKPPSQPRISGLPVSKSLREGERVQLNC-SSPDGSPRPRVDWYLGDSPNVIPESVYAKVPIPHSMINLTASRRDNERIYRCSARNDANVHKPSSTTITLQV 467          

BLAST of nephrin- partial vs. L. salmonis genes
Match: EMLSAG00000011552 (supercontig:LSalAtl2s:LSalAtl2s7:1384693:1561256:-1 gene:EMLSAG00000011552 transcript:EMLSAT00000011552 description:"maker-LSalAtl2s7-augustus-gene-15.10")

HSP 1 Score: 60.077 bits (144), Expect = 1.768e-9
Identity = 94/437 (21.51%), Postives = 165/437 (37.76%), Query Frame = 0

Query:  158 PPVMAVIINGDVVDAKMI----EVGHMVTLICKAQSGHPEPELAFYRN------SEQMGTARKMRNKFTFRASQRDN-NAVFTCTA-YNGFSLMSRSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGEN-EVSSRAMMDSLSITREFPPNRVSVTG----PAKIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHP-PAKIQWYMGS---QMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMNE 573
            PP    I+N +  D   I    EVG  V L C    G P PE+ ++R+      S +   ++ ++N  T  A ++D+    F C A  N  SL + + +TL++ F P +V I  PK  +  +  +   + C S  S P   I +  ++   G   T                         E+G    S+     T   K   NG ++ C  EN ++   A+ D   +T  + P  +   G    P  I     V F C  S   P  ++ WR      +  K  G                 + +    L+L+  ++ A     +  C AS+     D+I+    + V++ P   +I+   +G     G  R    + +   HP P +  W   +    +  S Y    ++  +++ + P  + +  +L C A N+
Sbjct:  122 PPSTPTILNENGEDINFIAGPYEVGSTVILKCVTNGGRPSPEVTWWRDHSLIDSSYEKSFSQTVKNTLTLLAIKKDDLGRKFRCQASNNNVSLPASTSITLDLLFRPTSVRIVTPKEPL--STSKVYKIKCMSLGSRPSATITWWRNNDFLGRTET------------------------WEEGNKTYSS----FTYTPKPNDNGKRLTCRAENTKLKGSAIEDVWKLTIYYIPEIILNFGANLNPNNIAEGNDVYFECNISSNPPVYKIVWRHNNKIVNHNKSKG-----------------IIISGTSLVLQEVKRNATG---SYSCTASN--TEGDIISNTITLRVMYKPVCSNINIQITG----IGKDRLANVSCNVHSHPLPQEFFWTFNNTDKSIDISKYNFSQNSTHSNLLYTPPEDIDYGKLYCWAQNK 502          

BLAST of nephrin- partial vs. L. salmonis genes
Match: EMLSAG00000004130 (supercontig:LSalAtl2s:LSalAtl2s218:18205:205970:-1 gene:EMLSAG00000004130 transcript:EMLSAT00000004130 description:"maker-LSalAtl2s218-augustus-gene-2.15")

HSP 1 Score: 59.3066 bits (142), Expect = 2.963e-9
Identity = 34/110 (30.91%), Postives = 59/110 (53.64%), Query Frame = 0

Query:  469 CFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQV---DGDTALAHITFVPQLEYNGSELRCEAMNEAI 575
            C AS+  L   +++  HL ++  PP   S++  +  +Q   G   E+ C S+    PPA+I WY+  Q ++   +    DG+T +  + F+P++E  G EL C+A+N  I
Sbjct:   58 CIASNTNLTRPIVSIVHL-DMNFPP--TSVNITNKIQQFVVGKKSEVHCRST-GSRPPAQIVWYLNGQRLDGGSERASPDGNTTVGVLQFLPKMEDIGKELGCKALNPLI 163          

BLAST of nephrin- partial vs. L. salmonis genes
Match: EMLSAG00000007484 (supercontig:LSalAtl2s:LSalAtl2s42:755998:900098:-1 gene:EMLSAG00000007484 transcript:EMLSAT00000007484 description:"maker-LSalAtl2s42-augustus-gene-8.16")

HSP 1 Score: 54.6842 bits (130), Expect = 8.759e-8
Identity = 50/180 (27.78%), Postives = 80/180 (44.44%), Query Frame = 0

Query:   24 AGIWSAEIPVCTKVGGCNPDDLAEIGNGRKIVV-------RRFRGSVVRYRCLGGYTIYG--QSRAHCLGS-EWSQTHPPICARIGCD--ESLLRG-IPHGQSKSLMKGEVFRFRCEPGAMLMGSATVFCDGK-QWNDTAPQCIIQPEPPVMAVIINGDVVDAKM-IEVGHMVTLICKAQ 188
            +G WS+ IP C  +      ++ E  N   IV        + + G+   + C  G+ + G   S A CL S +WS    P C  I C   E+   G +  G  K    G++ +FRC+ G M+ G     C+ +  W++ AP C+     P    +I G + D K   +VG  V+  C ++
Sbjct: 1109 SGSWSSPIPACMNI---TCPEIIEPNNSELIVRMSGIVDNKIYSGTKAYFSCSPGFYLEGXLSSEATCLRSGKWSIEPMPKCLPISCSSPENPESGFVNTGGKKFFHTGDMVKFRCKSGYMMEGQPVSRCNERGDWSNLAPTCVRACTYP--GSVIGGTISDVKFYYKVGDTVSFKCSSE 1283          

BLAST of nephrin- partial vs. SwissProt
Match: gi|1375381487|sp|Q96PZ7.3|CSMD1_HUMAN (RecName: Full=CUB and sushi domain-containing protein 1; AltName: Full=CUB and sushi multiple domains protein 1; Flags: Precursor)

HSP 1 Score: 66.2402 bits (160), Expect = 9.128e-10
Identity = 52/193 (26.94%), Postives = 83/193 (43.01%), Query Frame = 0

Query:    7 KCDPDFELVGKDLIKC-QAGIWSAEIPVCTKVGGCNPDDLAE-IGNGRKIVVRRFRGSVVRYRCLGGYTIYGQSRAHCLGSEWSQTHPPICARIGCDES--LLRGIPHGQS---KSLMKGEVFRFRCEPGAMLMGSATVFCDGKQ-WNDTAPQCII----QPEPPVMAVIINGDVVDAKMIEVGHMVTLICKA 187
            +C+P F LVG  +  C Q   WS + PVC  +   +P + A    NG +  +      VV + C  GY + G SRA C  +    +  P C  + C +   +   I HGQ    +S   G    + C+ G  L+GS+ + C     W+ + P+C+      P  P  AV+        ++   G +V   C+ 
Sbjct: 2705 QCNPGFRLVGTSVRICLQDHKWSGQTPVCVPITCGHPGNPAHGFTNGSEFNLN----DVVNFTCNTGYLLQGVSRAQCRSNGQWSSPLPTCRVVNCSDPGFVENAIRHGQQNFPESFEYGMSILYHCKKGFYLLGSSALTCMANGLWDRSLPKCLAISCGHPGVPANAVLT------GELFTYGAVVHYSCRG 2887          

BLAST of nephrin- partial vs. SwissProt
Match: gi|81885099|sp|Q6P1D5.1|SE6L1_MOUSE (RecName: Full=Seizure 6-like protein; AltName: Full=Acupuncture-induced protein 1-L; AltName: Full=Brain-specific receptor-like protein B; Short=BSRP-B; Flags: Precursor)

HSP 1 Score: 65.855 bits (159), Expect = 9.540e-10
Identity = 46/166 (27.71%), Postives = 73/166 (43.98%), Query Frame = 0

Query:    7 KCDPDFELVGKDLIKCQAGI-WSAEIPVCTKVGGCNPDDLAEIGNGRKIVVR--RFRGSVVRYRCLGGYTIYGQSRAHCLGSE-----WSQTHPPICAR--IGCDESLLRGIPHGQSKSLMK-----GEVFRFRCEPGAMLMGSATVFCDGKQ---WNDTAPQCII 154
            +CDP +++VG D + CQ  + WS++ P C K+  C   D  E+ +  +++       G+ ++Y C  G+ + G S   C   E     W+   P   +   + CD     G+P    + L K     GE   F C  G  LMG  T+ C   Q   W+   P C +
Sbjct:  712 QCDPGYDIVGSDTLTCQWDLSWSSDPPFCEKIMYCT--DPGEVEHSTRLISDPVLLVGTTIQYTCSPGFVLEGSSLLTCYSRETGTPIWTSRLPHCVSEESLACDNP---GLPENGYQILYKRLYLPGESLTFMCYEGFELMGEVTIRCILGQPSHWSGPLPICKV 872          

BLAST of nephrin- partial vs. SwissProt
Match: gi|341940567|sp|P08607.3|C4BPA_MOUSE (RecName: Full=C4b-binding protein; Short=C4bp; Flags: Precursor)

HSP 1 Score: 64.6994 bits (156), Expect = 1.247e-9
Identity = 52/179 (29.05%), Postives = 77/179 (43.02%), Query Frame = 0

Query:    8 CDPDFELVGKDLIKCQA-----GIWSAEIPVCTKVGGCNPDDLAEIGNGRKIVVRRFRGSV-----VRYRCLGGYTIYGQSRAHCLGS-EWSQTHPPICARIGCDESLLRGIPHGQSKSLMKGEV--FRFRCEPGAMLMGSATVFCDGKQWNDTAPQCIIQPEPPVMAVIINGDVVDAK 173
            CDP F LVG   I C        +WS+  P C K+    P+ L        ++V  ++ +      VR  CL G  + G+    C G+  WS    P C    CD  L   I +G   S++  ++    + C+ G  L+G A + C   +W  TAPQC    + P +    NG + D K
Sbjct:  209 CDPGFRLVGSPFIGCTVVNKTVPVWSSSPPTCEKIICSQPNIL------HGVIVSGYKATYTHRDSVRLACLNGTVLRGRHVIECQGNGNWSSL--PTC-EFDCD--LPPAIVNGYYTSMVYSKITLVTYECDKGYRLVGKAIISCSFSKWKGTAPQCKALCQKPEVG---NGTLSDEK 373          

BLAST of nephrin- partial vs. SwissProt
Match: gi|145559473|sp|P05160.3|F13B_HUMAN (RecName: Full=Coagulation factor XIII B chain; AltName: Full=Fibrin-stabilizing factor B subunit; AltName: Full=Protein-glutamine gamma-glutamyltransferase B chain; AltName: Full=Transglutaminase B chain; Flags: Precursor)

HSP 1 Score: 63.929 bits (154), Expect = 2.990e-9
Identity = 51/177 (28.81%), Postives = 79/177 (44.63%), Query Frame = 0

Query:    8 CDPDFELVGKDLIKCQAGIWSAEIPVCT-KVGGCNPDDLAEIGNGRKIVVRRFR-GSVVRYRCLGGYTIYGQSRAHCLGSEWSQTHPPIC----ARIGCDES--LLRGIPHGQSKSLMKGEVFRFRCEPGAMLMGSATVFCDGKQWNDTAPQCIIQPE----PPVMAVIINGDVVDA 172
            C  ++ L G DLI+C    W  E PVC  +   C P  L  I +  +     +R G +V   C   + I+G +   C   +W  T PP C     ++ C+E   +  G  +  SK    G+   + C+ G +L GS  + C+  +W    P+C+   E    PP   V++NG V D 
Sbjct:  241 CHENYYLSGSDLIQCYNFGWYPESPVCEGRRNRCPPPPLP-INSKIQTHSTTYRHGEIVHIECELNFEIHGSAEIRCEDGKW--TEPPKCIEGQEKVACEEPPFIENGAANLHSKIYYNGDKVTYACKSGYLLHGSNEITCNRGKWT-LPPECVENNENCKHPP---VVMNGAVADG 410          

BLAST of nephrin- partial vs. SwissProt
Match: gi|182705236|sp|Q61475.2|DAF1_MOUSE (RecName: Full=Complement decay-accelerating factor, GPI-anchored; Short=DAF-GPI; AltName: CD_antigen=CD55; Flags: Precursor)

HSP 1 Score: 63.1586 bits (152), Expect = 3.031e-9
Identity = 46/153 (30.07%), Postives = 68/153 (44.44%), Query Frame = 0

Query:   26 IWSAEIPVCTKVGGCNPDDLAEIGNGR-KIVVRRFRGSVVRYRCLGGYTIYGQSRAHCLGS----EWSQTHPPICARIGCDE--SLLRGIPHGQSKSLMKGEVFRFRCEPGAMLMGSATVFC-----DGKQWNDTAPQCIIQPEPPVMAVIIN 166
            +WS     C K    NP DL    NG   I      GS + + C  GY + G S   C  +    +W    P +C  I C E   +  GI  G+S S    +V  + C+ G +L+G+A+++C     D  QW+   P+CI + + P     IN
Sbjct:  150 VWSPVAQFCKKKSCPNPKDL---DNGHINIPTGILFGSEINFSCNPGYRLVGVSSTFCSVTGNTVDWDDEFP-VCTEIHCPEPPKINNGIMRGESDSYTYSQVVTYSCDKGFILVGNASIYCTVSKSDVGQWSSPPPRCIEKSKVPTKKPTIN 298          

BLAST of nephrin- partial vs. SwissProt
Match: gi|75052179|sp|O02839.1|MCP_PIG (RecName: Full=Membrane cofactor protein; AltName: CD_antigen=CD46; Flags: Precursor)

HSP 1 Score: 62.3882 bits (150), Expect = 5.600e-9
Identity = 56/180 (31.11%), Postives = 81/180 (45.00%), Query Frame = 0

Query:    1 MFVGKAK--CDPDFELVGKDLIKCQAG----IWSAEIPVCTKVGGCNPDDLAEIGNGRKIVVRR--FR-GSVVRYRCLGG-----YTIYGQSRAHCLGS-EWSQTHPPICARIGCDESLLRGIPHGQ------SKSLMKGEVFRFRCEPGAMLMGSATVFCDGKQ-WNDTAPQCIIQPEP 158
            +F  KA+  C+  F ++G + + CQ       WS   P+C K+  C P    EI NG+     +  F    VV Y CL       +++ G+S   C+G  EWS + PP C  + C   +   +P+G+      SK   K EV  F+C  G  L G  T+ C     W    PQCI   +P
Sbjct:  127 LFGSKAQFTCNTGFYIIGAETVYCQVSGNVMAWSEPSPLCEKIL-CKPP--GEIPNGKYTNSHKDVFEYNEVVTYSCLSSTGPDEFSLVGESSLFCIGKDEWS-SDPPECKVVKCPYPV---VPNGEIVSGFGSKFYYKAEVV-FKCNAGFTLHGRDTIVCGANSTWEPEMPQCIKDSKP 298          

BLAST of nephrin- partial vs. SwissProt
Match: gi|296439386|sp|Q2VPA4.3|CR1L_HUMAN (RecName: Full=Complement component receptor 1-like protein; AltName: Full=Complement C4b-binding protein CR-1-like protein; Flags: Precursor)

HSP 1 Score: 62.7734 bits (151), Expect = 7.432e-9
Identity = 52/186 (27.96%), Postives = 79/186 (42.47%), Query Frame = 0

Query:   12 FELVGKDLIKC-----QAGIWSAEIPVCTKVGGCNPDDLAEIGNGRKIVVRRFRGS---VVRYRCLGGYTIYGQSRAHCLGSEWSQTHPPICARIGCD---ESLLRGIPHGQSKSLMKGEVFRFRCEPGAMLMGSATVFCDGK-QWNDTAPQCIIQPEPPVMAVIINGDVVDAKMIEVGHMVTLIC 185
            FELVG+  I C     Q GIWS   P C     C P ++    NG  +   R   S   VV +RC  G+ + G S   C      +   P C+R+ C    + L          +   G+   + CEPG  L GS  + C  +  W+  AP+C ++     +  + NG V+    +++G  V  +C
Sbjct:  197 FELVGEPSIYCTSKDDQVGIWSGPAPQCIIPNKCTPPNVE---NGILVSDNRSLFSLNEVVEFRCQPGFGMKGPSHVKCQALNKWEPELPSCSRV-CQPPPDVLHAERTQRDKDNFSPGQEVFYSCEPGYDLRGSTYLHCTPQGDWSPAAPRCEVKSCDDFLGQLPNGHVLFPLNLQLGAKVDFVC 378          

HSP 2 Score: 60.077 bits (144), Expect = 4.279e-8
Identity = 61/201 (30.35%), Postives = 87/201 (43.28%), Query Frame = 0

Query:    3 VGKAKCDPDFELVGKDLIKCQA-GIWSAEIPVCTKVGGCN-PDDLAEIGNGRKIVVRRFRGSVVRYRCLGGYTIYGQSRAHCL-GSEWSQTHPPICARIGCDESLLRGIPHGQ---SKSLMKGEVFRFRCEPGAMLMGSATVFC--DGKQ--WNDTAPQCIIQP--EPPVMAVIINGDVVDAKMIEVGHMVTLICKAQSGH 191
            V + +C P F + G   +KCQA   W  E+P C++V  C  P D+      ++       G  V Y C  GY + G +  HC    +WS    P C    CD+  L  +P+G      +L  G    F C+ G  L GS+  +C   G +  WN + P C  +    PPV    +NG V     I VG  +   C   +GH
Sbjct:  253 VVEFRCQPGFGMKGPSHVKCQALNKWEPELPSCSRV--CQPPPDVLHAERTQRDKDNFSPGQEVFYSCEPGYDLRGSTYLHCTPQGDWSPAA-PRCEVKSCDD-FLGQLPNGHVLFPLNLQLGAKVDFVCDEGFQLKGSSASYCVLAGMESLWNSSVPVCERKSCETPPVP---VNGMVHVITDIHVGSRINYSC--TTGH 444          

BLAST of nephrin- partial vs. SwissProt
Match: gi|408360087|sp|Q07968.2|F13B_MOUSE (RecName: Full=Coagulation factor XIII B chain; AltName: Full=Protein-glutamine gamma-glutamyltransferase B chain; AltName: Full=Transglutaminase B chain; Flags: Precursor)

HSP 1 Score: 62.3882 bits (150), Expect = 9.937e-9
Identity = 50/178 (28.09%), Postives = 76/178 (42.70%), Query Frame = 0

Query:    8 CDPDFELVGKDLIKCQAGIWSAEIPVCT-KVGGCNPDDLAEIGNGRKIVVRRFRGSVVRYRCLGGYTIYGQSRAHCLGSEWSQTHPPIC----ARIGCDE--SLLRGIPHGQSKSLMKGEVFRFRCEPGAMLMGSATVFCDGKQWNDTAPQCIIQPE----PPVMAVIINGDVVDAKM 174
            C  ++ L G DLI+C    W  E P+C  +   C P  +      +        G  V   C   + I G     C   +W  T PP C     ++ C++  S+  G+ H  S+    G+   +RC  G  L GS+T+ C+  +W    P+C+   E    PP +A   NG VVD  +
Sbjct:  242 CHENYYLSGSDLIQCYNFGWYPESPICEGRRNRCPPPPVPLNSKIQPHSTTYRHGERVHIECELNFVIQGSEELLCENGKW--TEPPKCIEEKEKVACEQPPSVENGVAHPHSEIYYSGDKVTYRCGGGYSLRGSSTITCNRGRWT-LPPECVENIENCKPPPDIA---NGVVVDGLL 413          

BLAST of nephrin- partial vs. SwissProt
Match: gi|56405215|sp|P68638.1|VCP_VACCW (RecName: Full=Complement control protein C3; AltName: Full=28 kDa protein; AltName: Full=Secretory protein 35; Short=Protein C3; AltName: Full=VCP; Flags: Precursor >gi|56405216|sp|P68639.1|VCP_VACCC RecName: Full=Complement control protein C3; AltName: Full=28 kDa protein; AltName: Full=Secretory protein 35; Short=Protein C3; AltName: Full=VCP; Flags: Precursor)

HSP 1 Score: 60.077 bits (144), Expect = 1.327e-8
Identity = 48/156 (30.77%), Postives = 70/156 (44.87%), Query Frame = 0

Query:    8 CDPDFELVGKDLIKCQAG-----IWSAEIPVCTKVGGCNPDDLAEIGNGRKIVVRRFR--GSVVRYRCLGGYTIYGQSRAHCLGSEWSQTHPPICARIGCDESLLRG--IPHGQSKSLMKGEVFRFRCEPGAMLMGSATVFCD-GKQWNDTAPQCI 153
            C+  + L+G+    C+ G     +W+ E P+C  V   +P     I NGR      F   GSVV Y C  GY++ G S   C G EWS   PP C  + C    +    +  G  +S    +   F+C+ G  L GS++  C  G  W    P+C+
Sbjct:  112 CNSGYHLIGESKSYCELGSTGSMVWNPEAPICESVKCQSP---PSISNGRHNGYEDFYTDGSVVTYSCNSGYSLIGNSGVLCSGGEWSD--PPTCQIVKCPHPTISNGYLSSGFKRSYSYNDNVDFKCKYGYKLSGSSSSTCSPGNTWKPELPKCV 262          

HSP 2 Score: 54.6842 bits (130), Expect = 8.823e-7
Identity = 38/118 (32.20%), Postives = 56/118 (47.46%), Query Frame = 0

Query:   47 EIGNGRKIVVRRFRGSVVRYRCLGGYTIYGQSRAHC-LGSEWSQT---HPPICARIGCDE--SLLRGIPHGQSKSLMKGEVFRFRCEPGAMLMGSATVFCDGKQWNDTAPQCIIQPEP 158
            +I NG+  +     GS + Y C  GY + G+S+++C LGS  S       PIC  + C    S+  G  +G       G V  + C  G  L+G++ V C G +W+D  P C I   P
Sbjct:   91 DIDNGQLDIGGVDFGSSITYSCNSGYHLIGESKSYCELGSTGSMVWNPEAPICESVKCQSPPSISNGRHNGYEDFYTDGSVVTYSCNSGYSLIGNSGVLCSGGEWSD-PPTCQIVKCP 207          

BLAST of nephrin- partial vs. SwissProt
Match: gi|171769535|sp|A2AVA0.1|SVEP1_MOUSE (RecName: Full=Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1; AltName: Full=Polydom; Flags: Precursor)

HSP 1 Score: 62.003 bits (149), Expect = 1.771e-8
Identity = 44/149 (29.53%), Postives = 62/149 (41.61%), Query Frame = 0

Query:    7 KCDPDFELVGKDLIKCQA-GIWSAEIPVCTKVGGCNPDDLAEIGNGRKIVVRRFRGSVVRYRCLGGYTIYGQSRAHCLGSEWSQTHPPICARIGCDESLLRGIPHGQSKSLMKGEVFRFRCEPGAMLMGSATVFCDGK-QWNDTAPQCI 153
            +CD  + L G +   C A G WS   PVC  V    P+D   I NG+ I+      S+  Y C  GY++ G S   C  S      PP C  + C E  +         +   G    + C+ G  L G  T+ C    +WN +  QC+
Sbjct: 1871 RCDKGYTLSGDEESACLASGSWSHSSPVCELVKCSQPED---INNGKYILSGLTYLSIASYSCENGYSLQGPSLLECTASGSWDRAPPSCQLVSCGEPPIVKDAVITGSNFTFGNTVAYTCKEGYTLAGPDTIVCQANGKWNSSNHQCL 2016          

HSP 2 Score: 59.3066 bits (142), Expect = 1.434e-7
Identity = 56/185 (30.27%), Postives = 78/185 (42.16%), Query Frame = 0

Query:    8 CDPDFELVGKDLIKC-QAGIWSAEIPVCTKVGGCNPDDLAEIGNGRKIVVRRFRGSVVRYRCLGGYTIYGQSRAHCLG-SEWSQTHPPICARIGC--DESLLRGIPHGQSKSLMKGEVFRFRCEPGAMLMGSATVFC-DGKQWNDTAPQC--IIQPEPPVMAVIINGDVVDAKMIEVGHMVTLIC 185
            C     L G  ++KC  +G W+   P+C  V  C    L   G         F GS V+Y C+ G+ + G     C   S WS   P  C  + C   E +L GI H Q  + +   +  + C+PG  L+G+AT  C +  QW    P C  I  PEP     I+NG        + G  +T  C
Sbjct: 2348 CKEGHALQGPSVLKCLPSGQWNGSFPICKMVL-CPSPPLIPFGVPASSGALHF-GSTVKYLCVDGFFLRGSPTILCQADSTWSSPLPE-CVPVECPQPEEILNGIIHVQGLAYLSTTL--YTCKPGFELVGNATTLCGENGQWLGGKPMCKPIECPEP---KEILNGQFSSVSF-QYGQTITYFC 2523          

BLAST of nephrin- partial vs. nr
Match: gi|1325321669|ref|XP_023342649.1| (uncharacterized protein LOC111712306 [Eurytemora affinis])

HSP 1 Score: 221.861 bits (564), Expect = 6.333e-57
Identity = 164/613 (26.75%), Postives = 280/613 (45.68%), Query Frame = 0

Query:    2 FVGKAKCDPDFELVGKDLIKCQAGIWSAEIPVCTKVGGCNPDDLAEIGNGRKIVVRRFRGSVVRYRCLGGYTIYGQSRAHCLGSEWSQTHPPICARIGCDESLLRGIPHGQSKSLMKGEVFRFRCEPGAMLMGSATVFCDGKQWNDTAPQCIIQPEPPVMAVIIN---------GDV----VDAKMIEVGHMVTLICKAQSGHPEPELAFYRNSEQMGTARKMRNKFTFRASQRDNNAVFTCTAYNGFSLMSR---SDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVT-----GPA-KIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLET---GSPRELTCA---SSPAGHPPAKIQWYM-GSQMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRL 585
            +VG  KC   F LVG    KC+ G+WS+  PVCT VG C+P  L ++   R +  R FRGSV +Y C+ GY +YG+++ +C G+ W+ +  P+CA+ GCDE  +  + +G S+ L+ G V+RFRCE  A L+G   +FC+G  WN++ P C++    P + + IN         G+V    +   +++ G  V++ CKA  G+P+P +  Y + +Q+G  R+ RN F+F AS   +     C A N  S+M+    S + + VK+   ++ I  P   +  +L+   T++C++ PS P T++ +                  QD+ E  V+     N YS   GG+ TS+ + L             I C  ++      + +S +I    PP+   +      GP  K+K  +     C+     P   L  R+ +  + V      N  +  T E                         + D  +EC   +  L  + ++  HL  +L+P   P++     G ++ T   G+  E+ C        G+P A +   + G  ++E+  +   D  L    F  + ++ GS + C A+NE +    T +  +
Sbjct:   43 WVGTFKCQAGFVLVGAKRSKCRDGVWSSNPPVCTAVGACDPLSLPKVKFARLMEQRGFRGSVYKYTCMKGYRMYGKAKVYCDGTTWNFSTAPVCAKSGCDEDQVSVLRYGYSRRLLDGAVYRFRCEKEAELLGPDLIFCNGTLWNESTPTCLMPASSPNLTITINTEEEEVDKVGEVGEGDLKTVLVKKGDTVSVQCKAVDGNPDPLILIYFDEQQVGFPREFRNTFSFTASADQHGMRVRCEARN--SIMAAPAVSFMVIKVKYSASSLLISAPDFGLQGSLQ---TLTCTAGPSFPQTKLNWVYQ---------------QDVSESEVIID---NTYSEVDGGYYTSSKLQLRLPSSPLQSRSSNISCRSDDH--GDLLENSTTIQIYEPPSSPELLVLVDGGPVDKVKVGQQFTIECKVFGGNPDPIL--RLLVDKQEVGLSQNRNIIHTMTVE-----------------------ERNSDCEIECSVWNSALESEDVSKTHLA-ILYPAIQPTLRLAVDGIKVNTVTAGNTIEINCGYIDDKDRGNPEACLSLSIDGLSLVENSSE---DIVL---IFQSERDHQGSNIECSAINEVMETAATSSTEV 598          

BLAST of nephrin- partial vs. nr
Match: gi|1325328663|ref|XP_023346185.1| (complement receptor type 2-like, partial [Eurytemora affinis])

HSP 1 Score: 178.333 bits (451), Expect = 1.687e-47
Identity = 72/149 (48.32%), Postives = 101/149 (67.79%), Query Frame = 0

Query:    8 CDPDFELVGKDLIKCQAGIWSAEIPVCTKVGGCNPDDLAEIGNGRKIVVRRFRGSVVRYRCLGGYTIYGQSRAHCLGSEWSQTHPPICARIGCDESLLRGIPHGQSKSLMKGEVFRFRCEPGAMLMGSATVFCDGKQWNDTAPQCIIQP 156
            C P + LVG  L+KC  G+WS++ P C  +G C+P+DL EI NG+K+ V++FRG+V RY C  G+ IYGQ + HC G+ W     P+C+  GC+ESL+  +  G SK L +G ++R+ C+  A L+GS  +FCDGK WNDTAP+C I P
Sbjct:    4 CKPGYLLVGNRLLKCGRGVWSSKSPFCAAIGRCDPNDLPEIVNGQKVRVKQFRGAVYRYECENGFYIYGQKQVHCNGNSWDLRAAPVCSNTGCNESLVENLEFGFSKRLAEGGIYRYECKREATLIGSPMIFCDGKHWNDTAPRCTIGP 152          

BLAST of nephrin- partial vs. nr
Match: gi|1325328665|ref|XP_023346186.1| (uncharacterized protein LOC111715150 [Eurytemora affinis])

HSP 1 Score: 184.111 bits (466), Expect = 5.717e-45
Identity = 132/472 (27.97%), Postives = 221/472 (46.82%), Query Frame = 0

Query:    2 FVGKAKCDPDFELVGKDLIKCQAGIWSAEIPVCTKVGGCNPDDLAEIGNGRKIVVRRFRGSVVRYRCLGGYTIYGQSRAHCLGSEWSQTHPPICARIGCDESLLRGIPHGQSKSLMKGEVFRFRCEPGAMLMGSATVFCDGKQWNDTAPQCIIQPEPPVMAVIINGDVVDAKMIEVGHMVTLICKAQSGHPEPELAF----YR--NSEQMGTARKMR-----------NKFTFRASQRDNNAVFTCTAY--NGFSLMSRSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREF----PPNRVSVTGPAK-IKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGEN---PQYRPTTEGDVTVVSLTL 446
            ++G+  C P + LVG  L+KC  G+WS++ P C  +G C+P+DL EI NG+K+ V++FRG+V RY C  G+ IYGQ + HC G+ W     P+C+  GC+ESL+  +  G SK L +G ++R+ C+  A L+GS  +FCDGK WNDTAP+C I P         N  +     +++     L C+++   P  E+++    +R  N   +G   + +           +  T +  Q   + + TC+    N  +L+  + L LN ++PP  V + G     +    E + + C S+PS P + + + +               +QD    +VL Q        + G ++T +  LLS        + M + C   + +      D +S T       PP    ++ P K ++       +C +    P A L W           ++G+     QYR   EGD+ V +++ 
Sbjct:   37 WMGEFSCKPGYLLVGNRLLKCGRGVWSSKSPFCAAIGRCDPNDLPEIVNGQKVRVKQFRGAVYRYECENGFYIYGQKQVHCNGNSWDLRAAPVCSNTGCNESLVENLEFGFSKRLAEGGIYRYECKREATLIGSPMIFCDGKHWNDTAPRCTIGP--------ANVKISGPNDLKLNIKSELNCESEESVPPAEISWTIKDFRGNNLSHLGEVSQSKVYWVEKGWRTISTVTLKPMQAFPSLLVTCSGSTENEVNLVIDTKL-LNTQYPPETVRVYGQD---MGKDGEILLLECDSSPSYPPSSLSWRI---------------EQDGKSSIVLGQEILKRK--DDGSFMTRS--LLSIVASHDSNHSMVVKCSASHPL---LYDDQVSYTHTIQILCPPGVPKISSPVKSLEVGRQQQLSCYTKAGNPPALLQW-----------YNGDQLLESQYR--LEGDIAVANVSF 461          

HSP 2 Score: 176.407 bits (446), Expect = 2.085e-42
Identity = 190/701 (27.10%), Postives = 297/701 (42.37%), Query Frame = 0

Query:   66 YRCLGGYTIYGQSRAHCLGSEWSQTHPPICARIG-CDESLLRGIPHGQSKSL--MKGEVFRFRCEPGAMLMGSATVFCDGKQWN-DTAPQCIIQPEPPVMAVIINGDVVDAKMIEVGHMVTLICKAQSGHPEPELAFYRNSEQMGTARKMRNKFTFRASQRDNNAVFTCTAYNGFSLMSRSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAKIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRLDLTSNERFIEASTIGTTSTT--------------------STATTTTTTQSVPTTEETTTSSTTTTSRPIFPETSSSDHIYSNEHL------------ESYYDSNMAD-SGTEDIFEFLQSQTTASRQDLAPAENASDAYYYSDFHYDEYQ-DYDDEAYAY-PEDN----FSLEDKD 723
            + C  GY + G     C    WS +  P CA IG CD + L  I +GQ   +   +G V+R+ CE G  + G   V C+G  W+   AP C         +++ N +   +K +  G +    CK             R +  +G+         F   +  N+    CT                    P NV+I GP  L L N+K ++  +C S  S P   I + + D    N + +             +SQS    Y VE+G W T + + L   Q       + + C G  E     ++D+  +  ++PP  V V G    K  E ++  C SSP+ P + L+WR+E  GKS      E    +   +G     SL        L +      +  + V+C ASHP L +D ++Y H +++L PPG+P IS     + LE G  ++L+C +  AG+PPA +QWY G Q++ES Y+++GD A+A+++FV    Y   +  CEA N A+  PV ++I +++           I TTS+T                    +T   +TT       ++   +S+T  SR I+ E   S   Y NE +            + YY+  + +  G ++++E LQ  +        P EN           YDEYQ DY      Y P+ N    FS E K+
Sbjct:   41 FSCKPGYLLVGNRLLKCGRGVWS-SKSPFCAAIGRCDPNDLPEIVNGQKVRVKQFRGAVYRYECENGFYIYGQKQVHCNGNSWDLRAAPVC--SNTGCNESLVENLEFGFSKRLAEGGIYRYECK-------------REATLIGSP------MIFCDGKHWNDTAPRCT------------------IGPANVKISGPNDLKL-NIKSEL--NCESEESVPPAEISWTIKDFRGNNLSHLGE-----------VSQSKV--YWVEKG-WRTISTVTLKPMQA---FPSLLVTCSGSTENEVNLVIDTKLLNTQYPPETVRVYGQDMGKDGEILLLECDSSPSYPPSSLSWRIEQDGKSSIVLGQE--ILKRKDDGSFMTRSL--------LSIVASHDSNHSMVVKCSASHPLLYDDQVSYTHTIQILCPPGVPKISS--PVKSLEVGRQQQLSCYTK-AGNPPALLQWYNGDQLLESQYRLEGDIAVANVSFVL---YKPEKFTCEATNAAVNEPVRKSIEINV---------QYISTTSSTPEKKTTTFTTTTSTMISLRATTINNSTTLNGDLFKDQFRQNSSTKRSR-IYDEYDYSHFNYVNELMNQSFVETILEVGDEYYEDMLGEYDGNQEVYEDLQENSK------EPKEN-----------YDEYQKDYKTGQEVYQPKYNELLKFSEESKN 638          

BLAST of nephrin- partial vs. nr
Match: gi|1041547443|ref|XP_017302371.1| (PREDICTED: synaptogenesis protein syg-2-like, partial [Diaphorina citri])

HSP 1 Score: 155.221 bits (391), Expect = 6.402e-35
Identity = 126/441 (28.57%), Postives = 211/441 (47.85%), Query Frame = 0

Query:  156 PEPPVMAVIINGDVVDAKMIEVGHMVTLICKAQSGHPEPELAFYRNSE----QMGTARKM-RNKFTFRASQRDNNAVFTCTAYNGFSLMS-RSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHED--LVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAKIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMES-HYQVDGDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRLDL 587
            P+PP +     G+      I     V L+C+++ G+P  +L +Y+N E    Q  T+ +M  +  +F A   DN A F C A N  S    +++LTL V F P  V I GPK   + ++   V ++C++APSNP  +I + +      N T       Q+  E    VLS+S+ + +  E G                   N  K  C   N +S   +   L++T  F P  VS+TGP + +  + V  TC ++P+ P A++ W++  GG  V + + +N Q   ++EG  T  S T     +++     TA   +L V C+  +  L E      H + VL+PPG P I+G  + + L  GS  +++C SS  G+P A + WY   + + S   + D +T  + +T +  +  N +  +CEA N A  +P+ +T++  +
Sbjct:  214 PDPPYIEGYTEGET-----IRRAQTVELVCRSRGGNPPAQLTWYKNDETRRVQYRTSNRMSESTLSFVAEAGDNKAKFRCEASNVMSHTPLKTELTLTVFFAPATVSITGPKEARVGDI---VPLTCTTAPSNPPAQITWQIGGAKVRNAT-------QNTQEKTTCVLSESTLS-FVAEAGD------------------NKAKFRCEASNVMSHTPLKTELTLTVFFAPATVSITGPKEARVGDIVPLTCTTAPSNPPAQITWQI--GGAKV-RNATQNTQV--SSEGGWTSSSNT----SVVI-----TADMRNLVVVCYGHNKQLTEHATV-THTISVLYPPGPPIITGFDTSKPLIAGSIHKISCLSS-GGNPLANLVWYKNDKKINSVTKETDKNTVSSELTLLINVTDNEAIYKCEASNPATEIPLIETVQFSV 604          

HSP 2 Score: 90.8929 bits (224), Expect = 6.613e-15
Identity = 108/449 (24.05%), Postives = 178/449 (39.64%), Query Frame = 0

Query:  169 VVDAKMIEVGHMVTLICKAQSGHPEPELAFY----------RNSEQMGTARKMRNKFTFRASQRDNNAVFTCTAYNGFSLMS-RSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTG---PAKIKASESVVFTCQSSPAQPAARLAW-RVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYM-------GSQMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRLDLTSNERFIE 595
            +   K   VG +V L C     +P  ++ +           +N+++  T     +  +F A   DN A F C A N  S    +++LTL V F P  V I GPK   + ++   V ++C++APSNP  +I + +      N T                    +N     +GGW +S+N   ++  +   M  + + C G N+  +     + +I+  +PP    +TG      + A      +C SS   P A L W + +    SVTK            E D   VS  L    L++ +    A+      +C AS+P     LI       V  PP    IS     E L  G+   LTC +S + +PPA++ WY        G + +       G  +   +      + NG    C+A N A+   +   ++L++     F+E
Sbjct:  316 ITGPKEARVGDIVPLTCTTAPSNPPAQITWQIGGAKVRNATQNTQEKTTCVLSESTLSFVAEAGDNKAKFRCEASNVMSHTPLKTELTLTVFFAPATVSITGPKEARVGDI---VPLTCTTAPSNPPAQITWQIGGAKVRNAT--------------------QNTQVSSEGGWTSSSN---TSVVITADMRNLVVVCYGHNKQLTEHATVTHTISVLYPPGPPIITGFDTSKPLIAGSIHKISCLSSGGNPLANLVWYKNDKKINSVTK------------ETDKNTVSSELT---LLINVTDNEAI-----YKCEASNPATEIPLIETVQF-SVYFPPDHVHIS--RDPENLRAGTQATLTCDAS-SSNPPAQMSWYREGIPVLDGVRNLTKPGLHGGVISSIVLGLNVSHDVNGVTYTCQATNPALMRSINDAVQLNVMYKPVFLE 714          

BLAST of nephrin- partial vs. nr
Match: gi|1238842188|ref|XP_022239755.1| (nephrin-like [Limulus polyphemus])

HSP 1 Score: 137.887 bits (346), Expect = 1.777e-29
Identity = 112/470 (23.83%), Postives = 202/470 (42.98%), Query Frame = 0

Query:  146 NDTAPQCIIQPEPPVMAVIING--------------DVVDAKMIEVGHM--VTLICKAQSGHPEPELAFYRNSEQM-------GTARKMRNKF------TFRASQRDNNAVFTCTAYNGFSLMS-RSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAKIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRL 585
            +D   QC + P P   A+  N               D V+   IE+     ++L C    G P  E+ ++RN  ++        T      +F      T   S  DNNA++TC A +   +   +S L L+V +PPG   I G +        + +T+SC S   NP+ ++ +  +D                  + +  S ++    S  +  ++           + K  N     C   N +SS+     + ++  FPP+ V+V GP + K  E+V  TC +S + PAA + W V+        +   +P    TT  ++TV+   +++ D   ++            +CF  +  LGE ++  +H++ +L+PP  P+I G   G+ +  G  + +TC  +  G+P   ++W+ G Q ++S    +G+   + ++   +   NG+E RCEA N A   P+   I+L
Sbjct:   99 DDGEYQCQVGPTPDNHAIRANALLIVLLPPTSANFSDHVNGSTIEIHQFQSLSLTCTVTGGKPAAEIKWFRNDVELRPDDVETDTEEGENQRFNTISTITLTPSPDDNNAIYTCQAVHPALVQPLKSSLILSVLYPPGPPAIDGFQESETVRSGDTLTLSCISRGGNPLAQLVWFKND------------------QQIDFSYTTSGRKSTNKHTFV-----------VDKSDNHAIYRCEASNIISSQPANALVKLSVLFPPSEVAVNGPKEAKPGETVTMTCSASRSNPAAEINWLVDGQRVQGANYITPDPDGGWTTRSNITVI---IRSQDRGSKI-----------FQCFTVNKELGETMVE-SHVLSILYPPDPPTILGYTEGKSIRAGELQRITCVCN-GGNPLPTLKWFRGDQKVKSVTLTNGNVVNSEVSIETKESDNGAEFRCEADNSATDEPLVAKIKL 523          

HSP 2 Score: 101.293 bits (251), Expect = 3.776e-18
Identity = 115/461 (24.95%), Postives = 189/461 (41.00%), Query Frame = 0

Query:  156 PEPPVMAVIINGDVVDAKMIEVGHMVTLICKAQSGHPEPELAFYRNSEQMG-----TARKMRNKFTFRASQRDNNAVFTCTAYNGFSLMSRSDLT-LNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKI-DCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAK---IKASESVVFTCQSSPAQPAARLAW-RVEMGGKSVTKFSGENPQYRPTTEGDV--TVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDTALAHITFVPQL------EYNGSELRCEAMNEAIGVPVTQTIRLDLTSNERFIEAS 597
            P PP     I+G   +++ +  G  +TL C ++ G+P  +L +++N +Q+      + RK  NK TF   + DN+A++ C A N  S    + L  L+V FPP  V + GPK        E VT++CS++ SNP   I + +          + P  D                     GGW T +NI   T  ++    G KI  C   N+     M++S  ++  +PP+  ++ G  +   I+A E    TC  +   P   L W R +   KSVT            T G+V  + VS+  K  D                  C A +    E L+A   L     P G   ++     +  + G   +L C S+   +P + I W+    +++   +   D +    +   QL      E NG+   C+A NE +   V   + L++  N+R  + S
Sbjct:  235 PGPPA----IDG-FQESETVRSGDTLTLSCISRGGNPLAQLVWFKNDQQIDFSYTTSGRKSTNKHTFVVDKSDNHAIYRCEASNIISSQPANALVKLSVLFPPSEVAVNGPKEA---KPGETVTMTCSASRSNPAAEINWLVDGQRVQGANYITPDPD---------------------GGWTTRSNI---TVIIRSQDRGSKIFQCFTVNKELGETMVESHVLSILYPPDPPTILGYTEGKSIRAGELQRITCVCNGGNPLPTLKWFRGDQKVKSVT-----------LTNGNVVNSEVSIETKESDNGAEF------------RCEADNSATDEPLVAKIKLTVYFPPSG---VTIKVKPQNPKAGKKVKLICESA-TSNPASIITWWRDGFLIQGTPKGFSDASHGGKSTRNQLQLNVTSEDNGARYTCQATNEVLERSVHNAVTLNVMLNKRRTKQS 636          

HSP 3 Score: 72.0182 bits (175), Expect = 4.106e-9
Identity = 55/203 (27.09%), Postives = 89/203 (43.84%), Query Frame = 0

Query:  388 KIKASESVVFTCQSSPAQPAARLAW---RVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRLDL 587
            +I   +S+  TC  +  +PAA + W    VE+    V   + E    R  T   ++ ++LT   DD             +    C A HP L + L + + ++ VL+PPG P+I G    E + +G    L+C S   G+P A++ W+   Q ++  Y   G  +    TFV     N +  RCEA N     P    ++L +
Sbjct:  143 EIHQFQSLSLTCTVTGGKPAAEIKWFRNDVELRPDDVETDTEEGENQRFNT---ISTITLTPSPDD------------NNAIYTCQAVHPALVQPLKS-SLILSVLYPPGPPAIDGFQESETVRSGDTLTLSCISR-GGNPLAQLVWFKNDQQIDFSYTTSGRKSTNKHTFVVDKSDNHAIYRCEASNIISSQPANALVKLSV 328          

BLAST of nephrin- partial vs. nr
Match: gi|919086167|ref|XP_013380215.1| (nephrin isoform X3 [Lingula anatina])

HSP 1 Score: 130.183 bits (326), Expect = 4.906e-27
Identity = 117/455 (25.71%), Postives = 203/455 (44.62%), Query Frame = 0

Query:  153 IIQPEPPVMAVIINGDVVDAKMIEVGHMVTLICKAQSGHPEPELAFYRNSEQMG------------TARKMRNK--FTFRASQRDNNAVFTCTAYNGF--SLMSRSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPS---GDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAKIKASESVVFTCQSSPAQPAARLAWRVEMGGK--SVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHL-VEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRL 585
            ++ P+ P +    NG  V+  + ++   V L C A +G P P L + RN   +             +++++  K   T    + DN A+++C A +    + +  + + L+V + PG+  I G +   +    + VT+SC S   NP+ R+ +      Y N   +D S   GD     DLV++  + +                          N  +  C   N V+    + ++ +   F P +  + G A  +A E+++ +C SS + PAA++ W +  G +    T+   E P+    T  ++TV  LT   +D+I               EC A++  LG+  +A A + + VL+PP  P ISG  +G  +   S + L+C++S  G+P A I W+ G + + S  +  G  A+  I F+ Q   N +E RC A NEA   P+  T RL
Sbjct:  128 MVPPDQPEIEGHTNGSTVEVLLTDI--TVNLTCNAMNGKPAPGLIWKRNGRDVTETVQYSTYSSDPSSKRVNAKSIITLNPVKEDNGALYSCQATHPALRNKVLETVIKLSVLYEPGHPVITGYRTNQIIRTGDTVTLSCKSEGGNPLARVVW------YKNNIQIDYSFTTGDNMAVNDLVVNADASD--------------------------NNAEYKCEVSNVVTQAPKVAAVLMKVYFAPAKAEILGAANARAGETIMLSCVSSNSNPAAKITW-IAKGQQLSGATERVIEAPEGGYKTYSNLTV-PLTDSENDVIY--------------ECQATNEALGQ--MARADVTLRVLYPPNNPVISGYTAGTPIRAKSVQTLSCSAS-GGNPRATITWWKGDEELPSSDKSVGKIAIGEIQFIAQASDNLAEYRCNATNEATITPLLDTARL 529          

HSP 2 Score: 77.0258 bits (188), Expect = 1.149e-10
Identity = 103/442 (23.30%), Postives = 176/442 (39.82%), Query Frame = 0

Query:  173 KMIEVGHMVTLICKAQSGHPEPELAFYRNSEQMG----TARKMR-NKFTFRASQRDNNAVFTCTAYNGFSLMSR-SDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTG---PAKIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLA-VECFASHPGLGEDLIAYAHLVEVLHPPGL-----PSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMM--ESHYQVD----GDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRLDLTSNERF 593
            ++I  G  VTL CK++ G+P   + +Y+N+ Q+     T   M  N     A   DNNA + C   N  +   + + + + V F P   EI G          E + +SC S+ SNP  +I             T    G Q       LS ++       +GG+ T +N+   T  L    N +  +C   NE   +     +++   +PPN   ++G      I+A      +C +S   P A + W           + G+  +  P+++  V  ++        I  +      S +LA   C A++      L+  A L     P G+     P ++   +G++L       L C+SS +    + + W   ++ +   SHY  D    G + ++ +   P  E NG    CEA NE +   V   I+L++    RF
Sbjct:  253 QIIRTGDTVTLSCKSEGGNPLARVVWYKNNIQIDYSFTTGDNMAVNDLVVNADASDNNAEYKCEVSNVVTQAPKVAAVLMKVYFAPAKAEILGAANA---RAGETIMLSCVSSNSNPAAKI-------------TWIAKGQQ-------LSGATERVIEAPEGGYKTYSNL---TVPLTDSENDVIYECQATNEALGQMARADVTLRVLYPPNNPVISGYTAGTPIRAKSVQTLSCSASGGNPRATITW-----------WKGD--EELPSSDKSVGKIA--------IGEIQFIAQASDNLAEYRCNATNEATITPLLDTARLTVHFPPEGVTVTQDPPVA--RAGQRLT------LKCSSSSSNPA-SVVSWKKDNRPIGASSHYVQDAAYGGKSTISEVVLTPSEEDNGENYECEARNEELKQSVHNAIQLNVLFKPRF 638          

BLAST of nephrin- partial vs. nr
Match: gi|919086165|ref|XP_013380214.1| (nephrin isoform X2 [Lingula anatina])

HSP 1 Score: 130.183 bits (326), Expect = 5.025e-27
Identity = 117/455 (25.71%), Postives = 203/455 (44.62%), Query Frame = 0

Query:  153 IIQPEPPVMAVIINGDVVDAKMIEVGHMVTLICKAQSGHPEPELAFYRNSEQMG------------TARKMRNK--FTFRASQRDNNAVFTCTAYNGF--SLMSRSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPS---GDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAKIKASESVVFTCQSSPAQPAARLAWRVEMGGK--SVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHL-VEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRL 585
            ++ P+ P +    NG  V+  + ++   V L C A +G P P L + RN   +             +++++  K   T    + DN A+++C A +    + +  + + L+V + PG+  I G +   +    + VT+SC S   NP+ R+ +      Y N   +D S   GD     DLV++  + +                          N  +  C   N V+    + ++ +   F P +  + G A  +A E+++ +C SS + PAA++ W +  G +    T+   E P+    T  ++TV  LT   +D+I               EC A++  LG+  +A A + + VL+PP  P ISG  +G  +   S + L+C++S  G+P A I W+ G + + S  +  G  A+  I F+ Q   N +E RC A NEA   P+  T RL
Sbjct:  128 MVPPDQPEIEGHTNGSTVEVLLTDI--TVNLTCNAMNGKPAPGLIWKRNGRDVTETVQYSTYSSDPSSKRVNAKSIITLNPVKEDNGALYSCQATHPALRNKVLETVIKLSVLYEPGHPVITGYRTNQIIRTGDTVTLSCKSEGGNPLARVVW------YKNNIQIDYSFTTGDNMAVNDLVVNADASD--------------------------NNAEYKCEVSNVVTQAPKVAAVLMKVYFAPAKAEILGAANARAGETIMLSCVSSNSNPAAKITW-IAKGQQLSGATERVIEAPEGGYKTYSNLTV-PLTDSENDVIY--------------ECQATNEALGQ--MARADVTLRVLYPPNNPVISGYTAGTPIRAKSVQTLSCSAS-GGNPRATITWWKGDEELPSSDKSVGKIAIGEIQFIAQASDNLAEYRCNATNEATITPLLDTARL 529          

HSP 2 Score: 77.411 bits (189), Expect = 1.096e-10
Identity = 103/442 (23.30%), Postives = 176/442 (39.82%), Query Frame = 0

Query:  173 KMIEVGHMVTLICKAQSGHPEPELAFYRNSEQMG----TARKMR-NKFTFRASQRDNNAVFTCTAYNGFSLMSR-SDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTG---PAKIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLA-VECFASHPGLGEDLIAYAHLVEVLHPPGL-----PSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMM--ESHYQVD----GDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRLDLTSNERF 593
            ++I  G  VTL CK++ G+P   + +Y+N+ Q+     T   M  N     A   DNNA + C   N  +   + + + + V F P   EI G          E + +SC S+ SNP  +I             T    G Q       LS ++       +GG+ T +N+   T  L    N +  +C   NE   +     +++   +PPN   ++G      I+A      +C +S   P A + W           + G+  +  P+++  V  ++        I  +      S +LA   C A++      L+  A L     P G+     P ++   +G++L       L C+SS +    + + W   ++ +   SHY  D    G + ++ +   P  E NG    CEA NE +   V   I+L++    RF
Sbjct:  253 QIIRTGDTVTLSCKSEGGNPLARVVWYKNNIQIDYSFTTGDNMAVNDLVVNADASDNNAEYKCEVSNVVTQAPKVAAVLMKVYFAPAKAEILGAANA---RAGETIMLSCVSSNSNPAAKI-------------TWIAKGQQ-------LSGATERVIEAPEGGYKTYSNL---TVPLTDSENDVIYECQATNEALGQMARADVTLRVLYPPNNPVISGYTAGTPIRAKSVQTLSCSASGGNPRATITW-----------WKGD--EELPSSDKSVGKIA--------IGEIQFIAQASDNLAEYRCNATNEATITPLLDTARLTVHFPPEGVTVTQDPPVA--RAGQRLT------LKCSSSSSNPA-SVVSWKKDNRPIGASSHYVQDAAYGGKSTISEVVLTPSEEDNGENYECEARNEELKQSVHNAIQLNVLFKPRF 638          

BLAST of nephrin- partial vs. nr
Match: gi|919086163|ref|XP_013380213.1| (nephrin isoform X1 [Lingula anatina])

HSP 1 Score: 129.798 bits (325), Expect = 5.723e-27
Identity = 117/455 (25.71%), Postives = 203/455 (44.62%), Query Frame = 0

Query:  153 IIQPEPPVMAVIINGDVVDAKMIEVGHMVTLICKAQSGHPEPELAFYRNSEQMG------------TARKMRNK--FTFRASQRDNNAVFTCTAYNGF--SLMSRSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPS---GDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAKIKASESVVFTCQSSPAQPAARLAWRVEMGGK--SVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHL-VEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRL 585
            ++ P+ P +    NG  V+  + ++   V L C A +G P P L + RN   +             +++++  K   T    + DN A+++C A +    + +  + + L+V + PG+  I G +   +    + VT+SC S   NP+ R+ +      Y N   +D S   GD     DLV++  + +                          N  +  C   N V+    + ++ +   F P +  + G A  +A E+++ +C SS + PAA++ W +  G +    T+   E P+    T  ++TV  LT   +D+I               EC A++  LG+  +A A + + VL+PP  P ISG  +G  +   S + L+C++S  G+P A I W+ G + + S  +  G  A+  I F+ Q   N +E RC A NEA   P+  T RL
Sbjct:  128 MVPPDQPEIEGHTNGSTVEVLLTDI--TVNLTCNAMNGKPAPGLIWKRNGRDVTETVQYSTYSSDPSSKRVNAKSIITLNPVKEDNGALYSCQATHPALRNKVLETVIKLSVLYEPGHPVITGYRTNQIIRTGDTVTLSCKSEGGNPLARVVW------YKNNIQIDYSFTTGDNMAVNDLVVNADASD--------------------------NNAEYKCEVSNVVTQAPKVAAVLMKVYFAPAKAEILGAANARAGETIMLSCVSSNSNPAAKITW-IAKGQQLSGATERVIEAPEGGYKTYSNLTV-PLTDSENDVIY--------------ECQATNEALGQ--MARADVTLRVLYPPNNPVISGYTAGTPIRAKSVQTLSCSAS-GGNPRATITWWKGDEELPSSDKSVGKIAIGEIQFIAQASDNLAEYRCNATNEATITPLLDTARL 529          

HSP 2 Score: 77.0258 bits (188), Expect = 1.163e-10
Identity = 103/442 (23.30%), Postives = 176/442 (39.82%), Query Frame = 0

Query:  173 KMIEVGHMVTLICKAQSGHPEPELAFYRNSEQMG----TARKMR-NKFTFRASQRDNNAVFTCTAYNGFSLMSR-SDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTG---PAKIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLA-VECFASHPGLGEDLIAYAHLVEVLHPPGL-----PSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMM--ESHYQVD----GDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRLDLTSNERF 593
            ++I  G  VTL CK++ G+P   + +Y+N+ Q+     T   M  N     A   DNNA + C   N  +   + + + + V F P   EI G          E + +SC S+ SNP  +I             T    G Q       LS ++       +GG+ T +N+   T  L    N +  +C   NE   +     +++   +PPN   ++G      I+A      +C +S   P A + W           + G+  +  P+++  V  ++        I  +      S +LA   C A++      L+  A L     P G+     P ++   +G++L       L C+SS +    + + W   ++ +   SHY  D    G + ++ +   P  E NG    CEA NE +   V   I+L++    RF
Sbjct:  253 QIIRTGDTVTLSCKSEGGNPLARVVWYKNNIQIDYSFTTGDNMAVNDLVVNADASDNNAEYKCEVSNVVTQAPKVAAVLMKVYFAPAKAEILGAANA---RAGETIMLSCVSSNSNPAAKI-------------TWIAKGQQ-------LSGATERVIEAPEGGYKTYSNL---TVPLTDSENDVIYECQATNEALGQMARADVTLRVLYPPNNPVISGYTAGTPIRAKSVQTLSCSASGGNPRATITW-----------WKGD--EELPSSDKSVGKIA--------IGEIQFIAQASDNLAEYRCNATNEATITPLLDTARLTVHFPPEGVTVTQDPPVA--RAGQRLT------LKCSSSSSNPA-SVVSWKKDNRPIGASSHYVQDAAYGGKSTISEVVLTPSEEDNGENYECEARNEELKQSVHNAIQLNVLFKPRF 638          

BLAST of nephrin- partial vs. nr
Match: gi|1067080710|ref|XP_018024107.1| (PREDICTED: nephrin-like isoform X3 [Hyalella azteca])

HSP 1 Score: 128.642 bits (322), Expect = 1.505e-26
Identity = 105/416 (25.24%), Postives = 185/416 (44.47%), Query Frame = 0

Query:  174 MIEV--GHMVTLICKAQSGHPEPELAFYRNSEQMGTARK--------------MRNKFTFRASQRDNNAVFTCTA-YNGFSLMSRSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAKIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMN 572
            M+EV  G  VTL C  ++  P   + +YRN  ++    +              + +K T R S  D+  VF+C A +    L   + + ++V +PPG   I G       +  ++ T+SC+S   NP  ++ +      + N   +D S       D      S N+Y            ++ +++      NG    C   N+++   +  S+++  +FPP+ V VTGPA  +  E+V  TC +  + P A ++W V+       + SG+  + + +  G  T+ S+++             +V  D+   C A++  +G   +   + + VL PP  P + G   G  L+    + ++C S   G+PPAK+QWY   Q + S     GD + A I  + Q + NG++ RCEA N
Sbjct:  142 MLEVTEGEEVTLECLVEAAKPVASVIWYRNDRRIALETREDTQSESHLHRRHNLLSKITVRMSAEDHGTVFSCQAEHPALDLPKVASVRISVLYPPGPPIITGYSVGETISSGDERTLSCASRGGNPPAKVLW------FRNGENIDSS-------DRPFDGGSLNDY-----------KLVANSSD-----NGAVFTCQVMNKLTDVPLEASVTLDVQFPPDHVQVTGPAIARVGENVTLTCLTENSNPPATISWVVD---GQPHRSSGDRRE-KASAGGWHTISSISVIV----------PSVDRDMMFTCHATNQNIGTTKVD-TYTLSVLRPPQPPVLYGYSEGTGLQENKEQAISCVSR-GGNPPAKLQWYRNGQKIPSESHFVGDVSTAEIVLLAQAQDNGAQYRCEAFN 512          

HSP 2 Score: 74.3294 bits (181), Expect = 8.668e-10
Identity = 60/223 (26.91%), Postives = 99/223 (44.39%), Query Frame = 0

Query:  372 ITREFPPNRVSVTGPAK-----IKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLP-RQTAVSPDLAVECFASHPGLGEDLIAYAHL-VEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRLDL 587
            +T    P  V++ G  +     +   E V   C    A+P A + W      ++  + + E       T  D    S   +  +L+ ++  R +A        C A HP L  DL   A + + VL+PPG P I+G   GE + +G  R L+CAS   G+PPAK+ W+   + ++S  +     +L     V     NG+   C+ MN+   VP+  ++ LD+
Sbjct:  123 LTVLMAPTSVTLKGRGREDMLEVTEGEEVTLECLVEAAKPVASVIWY-----RNDRRIALE-------TREDTQSESHLHRRHNLLSKITVRMSAEDHGTVFSCQAEHPAL--DLPKVASVRISVLYPPGPPIITGYSVGETISSGDERTLSCASR-GGNPPAKVLWFRNGENIDSSDRPFDGGSLNDYKLVANSSDNGAVFTCQVMNKLTDVPLEASVTLDV 330          

BLAST of nephrin- partial vs. nr
Match: gi|1316180151|ref|XP_023235662.1| (nephrin-like isoform X2 [Centruroides sculpturatus])

HSP 1 Score: 128.257 bits (321), Expect = 1.661e-26
Identity = 102/434 (23.50%), Postives = 191/434 (44.01%), Query Frame = 0

Query:  166 NGDVVDAKMIEVGHMVTLICKAQSGHPEPELAFYRNSEQM-------------GTARKMRNKFTFRASQRDNNAVFTCTA-YNGFSLMSRSDLTLNVKFPPGNVEIQGPKRLILDNLKEDVTVSCSSAPSNPVTRIFFHLHDPSYGNKTTVDPSGDQDLHEDLVLSQSSRNEYSVEQGGWITSANILLSTTQLKKLMNGMKIDCVGENEVSSRAMMDSLSITREFPPNRVSVTGPAKIKASESVVFTCQSSPAQPAARLAWRVEMGGKSVTKFSGENPQYRPTTEGDVTVVSLTLKADDLILRLPRQTAVSPDLAVECFASHPGLGEDLIAYAHLVEVLHPPGLPSISGLHSGEQLETGSPRELTCASSPAGHPPAKIQWYMGSQMMESHYQVDGDTALAHITFVPQLEYNGSELRCEAMNEAIGVPVTQTIRL 585
            NG  V+ +  E    +T  C    G P  E+ +YR + ++                +   +  T   +  DN AV+TC A +   S+  R+ + L+V FPPG  EI+G +      + + +T+SC+S   NP+ ++ +      Y N+  VD S                  Y+        +  IL+  +      N     C   N VS + M  +  +T +F P +V+++G  + KA+++V   C + P+ P A ++W V+         +  +P     T  ++TV   T+   +  +++             C+A +  LGE ++  A ++ VL+PP  P+I G   G  + T +   +TC +   G+P   ++W+   + ++S    +G+   + ++ V +   NG+  RCEA N A   P+ + ++L
Sbjct:   60 NGSTVEVRESET---LTFTCVVGGGKPAAEIKWYRKNVELRPDNVEVSVEDADDHRQNAISTMTLYPNPDDNGAVYTCEAVHQALSVPLRTSVELSVLFPPGPPEIEGYQEGETIRMGDTLTLSCTSRGGNPLAQLIW------YKNEEQVDFS------------------YTTNGRESTNTITILVDASD-----NNALYKCEAFNVVSPKPMTATAKLTVQFAPAKVTISGSKEAKAADNVTMMCITDPSNPPAEISWVVDGRPIQSNSTTIADPGGGWVTSSNITV---TITGQERNMKM-----------FSCYAVNHALGETVVETA-VLSVLYPPDQPNIFGYAEGTPIRTDTLHRMTCVAH-GGNPLPSVKWFREGKEIKSTSTTNGNVVTSELSIVVKSSDNGAVFRCEATNSAAPEPLVRKVKL 445