BLAST of PREDICTED: pikachurin vs. L. salmonis genes
Match: EMLSAG00000007498 (supercontig:LSalAtl2s:LSalAtl2s430:288057:377591:1 gene:EMLSAG00000007498 transcript:EMLSAT00000007498 description:"maker-LSalAtl2s430-augustus-gene-4.9")

HSP 1 Score: 186.808 bits (473), Expect = 3.272e-51
Identity = 93/204 (45.59%), Postives = 124/204 (60.78%), Query Frame = 0

Query:  358 GGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEP 561
            GG G FRN GH+L   +GF GCI+RLEINNK+Y+F   E  GD+ FGMD  ECPED C  IPCQ+NG CV  + G      +    GTL+   ++         G+               +   +P+FNG+S+L Y G+ DSSPLW + E+V +S AEDGL+ YNG  NDG+ DF+A+ LSR +VEFA+D GDG+ +V +  P
Sbjct:  252 GGAGYFRNYGHYLGVETGFSGCIKRLEINNKVYDFRSKEHGGDSQFGMDITECPEDNCVLIPCQHNGKCVPLESG--HNIWWAINCGTLSISLRK---------GI---------------ISKNVPSFNGTSYLVYTGIGDSSPLWTEIELVFQSSAEDGLIFYNGQRNDGTDDFIAILLSRNYVEFAYDLGDGITVVRSASP 429          

HSP 2 Score: 114.775 bits (286), Expect = 6.225e-27
Identity = 67/158 (42.41%), Postives = 90/158 (56.96%), Query Frame = 0

Query:   70 VAIQIEVDIDPPPHQPNDPSDAELEKLGLPTHHVHYSTRTAPGESGSPQPGSESVIRPITSDEAEPNLAPDPPRALPSLSGVAAWPAHSEPKLNQPSGSRNQLPEADGDYKDKVYEIDAFCNLECGVEGECFMTRDDK--NVIKKRCLCPHGKYGEKC 225
            +AIQIEVDIDPP    ND S+AE++ LGLP+HHVHY T ++    GS  P  E ++ P    + +     D  ++              E +LN+         E +  Y+DK++EIDA+CN++CG  G CFM R  K   VI KRCLCP GK+GEKC
Sbjct:  117 IAIQIEVDIDPPQRGKNDLSEAEMKSLGLPSHHVHY-TSSSIETLGSSSPIKEELLEP---GDIQVTYENDDDKS-------------EEGRLNEG--------EEEEFYRDKMFEIDAYCNMDCGDRGSCFMERVQKEEEVIMKRCLCPLGKHGEKC 249          

HSP 3 Score: 81.6481 bits (200), Expect = 1.734e-16
Identity = 80/337 (23.74%), Postives = 135/337 (40.06%), Query Frame = 0

Query:  234 PRFSGHSYLALPTLTNAYSDLQLSVE--FRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEE--------GRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV---EVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVS 557
            P F+G SYL    + ++ S L   +E  F+  +E+G++   G+  D T D++A+++   ++E   D G G  +VR+   + LH W  + + R      +++  G  E+          S G FS++T  Q + LGG  +F     +L     F GCI++L IN+   N   S      + G+D   C        PC       S +       V G     L       D F       C CP G +G  C++        +P F  S +   F   +                + G+V          GD++AL +  G ++  ++ G G A ++
Sbjct:  352 PSFNGTSYLVYTGIGDS-SPLWTEIELVFQSSAEDGLIFYNGQRNDGTDDFIAILLSRNYVEFAYDLGDGITVVRSASPISLHVWYTVVISRTGRLAEMEVIRGKREDPLKMPVSSRVSNGAFSQLTLEQNLYLGGVPTFDLVSAYLPIRRSFFGCIQKLTINDVEINLVNS-----AISGVDVGSCDH------PCNTPSSSSSNEFPQHPCGVHGNCKPVL-------DGFK------CDCPHGLTGNSCQKITGESNNPLPRFKKSKNALIFWAGNGRNF------------KKGIV----------GDYIALGIYNGRLQLKYNLGSGEASIT 641          

BLAST of PREDICTED: pikachurin vs. L. salmonis genes
Match: EMLSAG00000003993 (supercontig:LSalAtl2s:LSalAtl2s213:441137:447252:-1 gene:EMLSAG00000003993 transcript:EMLSAT00000003993 description:"maker-LSalAtl2s213-snap-gene-4.21")

HSP 1 Score: 86.6557 bits (213), Expect = 7.166e-18
Identity = 101/410 (24.63%), Postives = 160/410 (39.02%), Query Frame = 0

Query:   98 LPTHHVHYSTRTAPGESGSPQPGSESVIRPITSDEAEPNLAPDPPRALPSLSGVAAWPAHSEPK-LNQPSGSRNQLPEADG-----DYKDKVYEIDAFCNLE-CGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAY---SDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIV---RTR-DQVHLHRWN-RLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHL 492
            L T  V Y T T    + S       +I P+  D    + + D     P +   A+  AH E + + +     + + + DG     D +   +  D  C    C   G C  T     +   +C+CP   +G +C  + +I +P F GHS L      N      +  + + FR   EEG++L TGE  D+    ++  I+DG +  ++ CG    +    R R D  +L   +  +T+F       +QLNE     G  Q + S       + +G     +  G+ +  N GF GC++ L++N+   N        D + G D  EC    C+  PC+N  IC   +  G                              C CP GF+G  CE+PV    P   G + L
Sbjct:  379 LATEEVDYKTTTKDDSNDS------GLILPV--DSTTISSSKDEETLRPEIGSTASGSAHEEEESMARNITYHHTITQEDGILQPFDNRSVEHFNDLICTSNVCSNGGTCLTT-----LSGVKCICPLQYHGVQCEEEVFIETPGFVGHSGLVHKLSKNNIVKDRNFHVQLSFRTSQEEGLILFTGE--DVGYLLVSGYIKDGILTFKISCGHQIILFSDPRHRVDXGYLQXLSINITIFDTICSTSIQLNETRTMSGDQQ-IDSLPQIPDMLYMGLVPHKKELGNGI-INVGFQGCMKHLQLNSNELNLYR-----DAVDGRDIVECKSAICAIQPCRNGAICAQHEDSGS---------------------------WFCDCPPGFTGALCERPVCQTNPCQYGGTCL 739          

HSP 2 Score: 71.633 bits (174), Expect = 3.681e-13
Identity = 97/401 (24.19%), Postives = 151/401 (37.66%), Query Frame = 0

Query:  194 CGVEGECFMTRDDKNVIKKRCLCPHGKYGEKC----ALDRYISSPRFSGH----------------------SYLALPTLT-NAYSDLQLSVEFRPVSEEGVLLL--TGESA--DMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVF-RHDWGVWLQLNEGVHEEGRSQGL--FSRITFAQPVLLGG--TGSFRNTGHFLDTNSGFHGCIRRLEINNKIYN-----FEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGV-CQCPLGFSGEY-CEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGS-GDFLALFLSRGFVEFAFDNG 550
            C   G C  ++     +   CLC  G+ G  C     +  YI    +  +                      SYLA P  T N    L+    F   + + V LL   G+    D   D++AL    GHI +  D G GP  + T+  V    +     F R+    WLQ++   +  G S G   F  +T +    +GG    +F    H    + GF GC+  +   + +         PS+     + G + ++C  D CSS PC N G C+   G     +   G +G   S++            V  +C +   G Y C  P   K P F G        L  +        + +R +  +G +++ G + +G   DFL+L L  G V F+F  G
Sbjct:  732 CQYGGTCLGSKTGSGFL---CLCQAGRRGSLCEEGNQIQVYIKRLNYKAYEYFLPEVELTQPSFRSSVGGYSSYLAYPVHTQNVLKSLEWQFHFSTPNTDQVALLFFLGQRGIHDFGSDFIALSYIKGHILMTWDLGAGPRRIFTKRPVDERYFVHAVRFGRNGRRGWLQVDNFPNITGLSPGKREFLNVT-SMYYYIGGHENYNFSLLPHDFPVHRGFSGCMFDMAYRSYVTGQLTPLALPSKIGSSPIRGRNVEQCHYDICSSSPCSNGGTCI-GXGASFLCECTDGRYGITCSEDTSACTTGMHKCAVSSKCTVTLKGSYECICPFG-KNPLFTGHGSYMQTHLKSAIRFNTHIHIEIRPQTLEGHIIHLGQSRNGRHQDFLSLLLVNGSVVFSFSLG 1126          

HSP 3 Score: 66.6254 bits (161), Expect = 1.429e-11
Identity = 74/316 (23.42%), Postives = 122/316 (38.61%), Query Frame = 0

Query:  175 ADGDYKDKVYEIDAFCNL---ECGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGH-SYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESAD-MTGDYLALIIRDGHIELRLDCG------TGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSG--FHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQ 477
             DG Y     E  + C     +C V  +C +T          C+CP GK            +P F+GH SY+     +    +  + +E RP + EG ++  G+S +    D+L+L++ +G +      G       G  +      V + +W  +   R+    +L+  +G    G ++   + +   Q + LGG  +      F+D  S   + GCIR L IN K Y   P    G+   G +  +C    C    CQN G C                  +L+     +  +       C CP  + GE+CEQ
Sbjct: 1013 TDGRYGITCSEDTSACTTGMHKCAVSSKCTVTLKGS----YECICPFGK------------NPLFTGHGSYMQTHLKSAIRFNTHIHIEIRPQTLEGHIIHLGQSRNGRHQDFLSLLLVNGSVVFSFSLGGPIGGNVGNTVTIRTCCVEIDKWYSVEAGRYGRKGYLR-XDGQFTTGSTESGLNTLDVDQELFLGGIPNSHML--FIDFVSKHYYEGCIRNLHINEKSY---PLTSTGNHWKGWNIKDCDGTACGGELCQNGGKC------------------SLSENIHNHRGYE------CGCPDNYIGEHCEQ 1282          

BLAST of PREDICTED: pikachurin vs. L. salmonis genes
Match: EMLSAG00000004167 (supercontig:LSalAtl2s:LSalAtl2s220:203389:278765:1 gene:EMLSAG00000004167 transcript:EMLSAT00000004167 description:"maker-LSalAtl2s220-augustus-gene-2.5")

HSP 1 Score: 69.707 bits (169), Expect = 1.168e-12
Identity = 57/250 (22.80%), Postives = 106/250 (42.40%), Query Frame = 0

Query:  214 CLCPHGKYGEKCALDRYIS---SPRFSGH-SYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTG------------------DYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGT---------GSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGG 432
            C C  G  G +C  D  ++   +  F+G  S++ +   T++   +++ + FR  + +  LLL G+S +++                   +   +     HI    D G+G   +++  ++   +W  L   R+     L+L       G ++G    +     + +GG             +N G    T+ GF GCI+ L+IN K  + + S R+   +      EC E++CS  PC+N G+C+S   G
Sbjct:  551 CYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTFPFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAG----TSIGFVGCIKELKINKKTISLQ-SMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQG 795          

BLAST of PREDICTED: pikachurin vs. L. salmonis genes
Match: EMLSAG00000003184 (supercontig:LSalAtl2s:LSalAtl2s176:575372:647076:1 gene:EMLSAG00000003184 transcript:EMLSAT00000003184 description:"augustus_masked-LSalAtl2s176-processed-gene-6.1")

HSP 1 Score: 54.6842 bits (130), Expect = 6.005e-8
Identity = 60/270 (22.22%), Postives = 113/270 (41.85%), Query Frame = 0

Query:  198 GECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRF-SGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGP-GIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGH------FLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTL---FGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDV----FGGAFGTLTSQEQE 452
            G CF      N I   C C  G  G++C    Y++S  F    SY+ +  L N      +++ F    + GVLL TG++      +LA+ +  G I +  D G  P   + + + +   +++R+ +        L+++ G+     ++G    +     + + G     + GH       L  ++ F GCI+ + +N+K+ +F  + +    +     +  DE P D C    C++ G C S   G   E +    F G F  +  + + 
Sbjct:  504 GICFQPPGSNNYI---CKCAPGYSGKRC---EYLTSLSFIHNTSYVEMEAL-NVKPKANITIIFATEQQNGVLLYTGDN-----QHLAVELFRGRIRVSYDVGNYPVSTMFSYELLSDGKYHRVELLAIKKNFTLRVDGGIARSIVNEGNNEYLEIRNSLFIAGVP--EDVGHKSLKQWHLRNSTSFKGCIKEVYVNDKLSDFLQAAKVRHKVTPGCSLYQDEQPTDPCVDHKCKHEGECRSKNDGTSYECICKPHFTGHFCQIRGKSKR 759          

BLAST of PREDICTED: pikachurin vs. L. salmonis genes
Match: EMLSAG00000001472 (supercontig:LSalAtl2s:LSalAtl2s124:357996:426231:-1 gene:EMLSAG00000001472 transcript:EMLSAT00000001472 description:"maker-LSalAtl2s124-augustus-gene-6.17")

HSP 1 Score: 53.9138 bits (128), Expect = 1.002e-7
Identity = 83/453 (18.32%), Postives = 171/453 (37.75%), Query Frame = 0

Query:  160 PKLNQPSGSRNQLPEADGDYKDKVYEIDAFCNLECGVEGECFMTRDDKNVIKKRCLCPH----GKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRD----QVHLHRWNRLTV-----FRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGT--GSFRNTGHFLDTNSGFHGCIRRLEIN--------------------------NKIYNFEPSE-----------RQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFG--TLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSN 558
            P L +  GS + L     +   ++Y  ++       ++   F  R+   +I+   L       G + +    D Y      S H+Y+ LP L   Y+++ L    R +  +G+++    +A    D++A+ + +GHI   L+ G   G++  RD    +++ ++W+ +++     F+H   V     +  H    S+G  S +     + LGG     +      + +  G+ GC+  +E+N                          N   N+E S+           R+          E      S   C+N+GIC+   G     D    +F     T +   Y+  N+P L      + ++  Y ++P           + +    L   +PL  ++ +++R ++              S DFL + +  G++   ++ G    ++S+
Sbjct:  410 PILAEAYGSMSXLGHTVLNLASRLYYTESLTPFSGHMQQFIFNGREYFEMIRSGTLVSSYSLTGVFYDANDSDVYHDVTFLSHHTYMGLPQL-KTYANIDLYFRLRTLETDGIIMF---NAGRGQDFIAVELSNGHIHYVLNLGY--GVINLRDNSPRKINDNKWHTVSIGRPSQFKHTLRV-----DTYHSSANSKGNESNLDLDGILFLGGVRKNMYSKLPSEIISTYGYQGCLACIELNGVAGDPINKALVPSNQVSKGCEEEARNSRNNYELSDIDTQYVGLNYHRKQQIQNHFRQQELDSSIISRCSCENDGICIEHGGSSPTCDCDLTSFSGPRCTEESVAYEWSNEPGL------IRYNFPYDKRP----------DTRIDVLSLGIITPL--NYAIIVRIDSA------------SSNDFLEMQIEDGYIHILYNLGTEDIMISD 821          

BLAST of PREDICTED: pikachurin vs. L. salmonis genes
Match: EMLSAG00000012352 (supercontig:LSalAtl2s:LSalAtl2s903:176857:182009:1 gene:EMLSAG00000012352 transcript:EMLSAT00000012352 description:"maker-LSalAtl2s903-augustus-gene-1.45")

HSP 1 Score: 50.8322 bits (120), Expect = 1.873e-7
Identity = 37/136 (27.21%), Postives = 64/136 (47.06%), Query Frame = 0

Query:  255 QLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLH--RWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNK 388
            + +V  R + + G+L+   +   + GD+LA+ I DG+ EL    G     +R + +  ++  +W+RL V R      +Q+++     G+S    S +     V LGG       G       GF GCIR+++I  K
Sbjct:   51 KFTVHLRTIEKNGLLIWINQGTSLKGDFLAVAIVDGYPELSYKLGKKQNTLRIKSKKRINDDKWHRLKVIRRRRTGLIQVDKLKPIRGKSGTGASILNTNGKVWLGGKEPL-PPGLPHQYYKGFKGCIRKVKIFRK 185          

BLAST of PREDICTED: pikachurin vs. L. salmonis genes
Match: EMLSAG00000003115 (supercontig:LSalAtl2s:LSalAtl2s174:638829:676917:-1 gene:EMLSAG00000003115 transcript:EMLSAT00000003115 description:"maker-LSalAtl2s174-augustus-gene-6.13")

HSP 1 Score: 51.9878 bits (123), Expect = 3.593e-7
Identity = 69/305 (22.62%), Postives = 119/305 (39.02%), Query Frame = 0

Query:  256 LSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVR-TRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTG------SFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPE--DTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCP-LGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNG 550
            +S++FR     G+LL  G   +   DY A  I DGH+ + LD G+GP  +R +R  ++   W+ + +        ++++E   E  ++ G  +++     + +GG         F  T   +    GF GC+R L IN    +      Q D   G     C +  D C   PC + G C                        + ++ F      +C C    F+G  C       I  F GS  ++      ++   +D  +  RS  + G++     ++D S D L + +  G V  +F  G
Sbjct:  509 ISLKFRTNELNGILLFNG--GNPHKDYFAFEILDGHLYVHLDLGSGPVKIRASRTPLNDGAWHHVELTLSQRSGRIKIDED-SESFKTPGEANQLDLVGNLFVGGVDYMDPYMKFPPTLWTVTLKYGFVGCLRDLTINGASIDVVSYAHQQDV--GSIRSSCHKMPDQCHDRPCNHGGKC-----------------------SEGWNRF------LCDCSRTSFTGATCGYAAATVI--FEGSHSISLSYKDGTTTEADDIILRFRSMEQHGILF--TTHHDTSKDKLDVTMESGRVRLSFQVG 775          

BLAST of PREDICTED: pikachurin vs. SwissProt
Match: gi|158705693|sp|A3KN33.1|EGFLA_BOVIN (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor)

HSP 1 Score: 210.305 bits (534), Expect = 3.753e-57
Identity = 143/451 (31.71%), Postives = 211/451 (46.78%), Query Frame = 0

Query:  122 ESVIRPITSDEAEPNLAPDPPRALPSLSGVAAWPAHSEPKLNQPSGSRNQLPEADGDYKDKVYEIDAFCNLECGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPL-------WNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQG 565
            ES + P  +      L P  P +LP+ + VA  P   + K         +L +   D  + V   D+FC               D      RC C  GK GE C+ D  I  P+F GHSY+    L N+Y   Q+++EFR  +E+G+LL  GE+    GD+++L +    ++ R +CGTG  I+ +  ++ L  W+ +T++R      LQLN G    G+SQG +S+ITF  P+ LGG  S         TN GF GC++ L +N K  +  P    G  L G D  EC    C    C N G C++++                       D +      +C CPLGF G +CE    + IP F   S  +Y   A   PL       + +FEV  R ++EDG++LY  + + GS DFL++ ++ G VEF FD G G  ++ + EP   G
Sbjct:  302 ESKMAPRPNPMTVSRLVPPTPASLPT-TAVAPQPTPVDRKGKHGVAVMPRLFDTSCD--ETVCSADSFC-------------VSDYTWGGSRCHCNLGKGGESCSEDIVIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYCGENEHGRGDFMSLAVIRRSLQFRFNCGTGVAIIVSETKIKLGGWHTVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQALTVNGKRLDLRPWP-LGKALSGADVGECSSGICDEASCINGGTCMASKA----------------------DSY------ICLCPLGFRGRHCEDAFTLTIPQFK-ESLRSY--AATPWPLEPRHYLSFMEFEVTFRPDSEDGVLLY--SYDTGSKDFLSINMAGGHVEFRFDCGSGTGVLRSEEPLTLG 702          

HSP 2 Score: 134.035 bits (336), Expect = 1.389e-31
Identity = 108/404 (26.73%), Postives = 197/404 (48.76%), Query Frame = 0

Query:  194 CGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRF--SGHSYLALP---TLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSG----FHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV--EVKIPAFNGSSHLTYFG---LADSSPLWNDFEVVLRSEAEDGLVLYNGNN-NDGSGDFLALFLSRGFVEFAFDNGDGVA--LVSNPEPECQGEQILALKPRSSGSLV 580
            C   G C  ++ D  +    CLCP G  G  C     ++ P+F  S  SY A P      +  S ++  V FRP SE+GVLL + ++   + D+L++ +  GH+E R DCG+G G++R+ + + L  W+ L V R      LQ+++    EG ++G F++I     + +GG  ++ +    +  NSG    F G I+++ +N++  +      + D  +G++ +      C   PC + G C                      +++ Y+         C CPLGF G +C++ +   ++IP F G S+LTY     L   S   ++  +  ++ A+DGL+L+ G++    + DF++L L  G + F+++ G GVA  +V+    + +  ++ A++   SG + 
Sbjct:  583 CINGGTCMASKADSYI----CLCPLGFRGRHCEDAFTLTIPQFKESLRSYAATPWPLEPRHYLSFMEFEVTFRPDSEDGVLLYSYDTG--SKDFLSINMAGGHVEFRFDCGSGTGVLRSEEPLTLGHWHELCVSRTAKNGILQVDKQKAVEGMAEGGFTQIKCNSDIFIGGVPNYDD----VKKNSGILKPFSGSIQKIILNDRTIHV-----KHDFTWGVNVENAAH-PCVGSPCAHGGSC--------------------RPRKEGYE---------CDCPLGFEGLHCQKAITEAIEIPQFIGRSYLTYDNPDILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKIT 941          

BLAST of PREDICTED: pikachurin vs. SwissProt
Match: gi|81908066|sp|Q4VBE4.1|EGFLA_MOUSE (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; AltName: Full=Nectican; Flags: Precursor)

HSP 1 Score: 202.986 bits (515), Expect = 1.519e-54
Identity = 121/361 (33.52%), Postives = 179/361 (49.58%), Query Frame = 0

Query:  213 RCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPL-------WNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQGE 566
            RC C  GK GE C+ D +I  P+F GHSY+    L N+Y   Q+++EFR  +E+G+LL  GES    GD+++L +    +  R +CGTG  I+ +  ++ L  W+ +T++R      LQLN G    G+SQG +S+ITF  P+ LGG  S         TN GF GC++ L +N K  +  P    G  L G D  EC    C    C + G C + +                       D +      +C CPLGF G +CE    + IP F  S  L  +  A   PL       + +FE+  R ++ DG++LY  + + GS DFL++ ++ G VEF FD G G  ++ +  P   G+
Sbjct:  368 RCHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQVTLEFRAEAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGIAIIISETKIKLGAWHTVTLYRDGLNGMLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLSVNGKKIDMRPWP-LGKALNGADVGECSSGICDEASCIHGGTCAAIKA----------------------DSY------ICLCPLGFRGRHCEDAFALTIPQFRES--LRSYA-ATPWPLEPQHYLSFTEFEITFRPDSGDGVLLY--SYDTGSKDFLSINMAAGHVEFRFDCGSGTGVLRSEAPLTLGQ 694          

HSP 2 Score: 118.242 bits (295), Expect = 2.055e-26
Identity = 105/408 (25.74%), Postives = 188/408 (46.08%), Query Frame = 0

Query:  198 GECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRF--SGHSYLALP---TLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSG----FHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV----------EVKIPAFNGSSHLTYFG---LADSSPLWNDFEVVLRSEAEDGLVLYNGNN-NDGSGDFLALFLSRGFVEFAFDNGDGVA--LVSNPEPECQGEQILALKPRSSGSLV 580
            G C   + D  +    CLCP G  G  C     ++ P+F  S  SY A P      +  S  +  + FRP S +GVLL + ++   + D+L++ +  GH+E R DCG+G G++R+   + L +W+ L V R      LQ+++    EG ++G F++I     + +GG  ++ +    +  NSG    F G I+++ +N++  + +     G  +      E     C   PC + G C                      +++ Y+         C CPLGF G  C++             ++IP F G S+LTY     L   S   ++  +  ++ A+DGL+L+ G++    + DF++L L  G + F+++ G GVA  +V+    + +  ++ A++   SG + 
Sbjct:  578 GTCAAIKADSYI----CLCPLGFRGRHCEDAFALTIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYSYDTG--SKDFLSINMAAGHVEFRFDCGSGTGVLRSEAPLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDD----VKKNSGILHPFSGSIQKIILNDRTIHVKHDFTSGVNV------ENAAHPCVGAPCAHGGSC--------------------RPRKEGYE---------CDCPLGFEGLNCQKECGNHCLNTIIEAIEIPQFIGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALIFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKIT 940          

HSP 3 Score: 75.0998 bits (183), Expect = 7.925e-13
Identity = 49/165 (29.70%), Postives = 76/165 (46.06%), Query Frame = 0

Query:  394 PSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVL--RSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALV 556
            P++R+G     M      + +C    C  +  CV+    GG                             C C LG  GE C + + ++ P F G S++T+  L +S   +  F+V L  R+EAEDGL+LY G +  G GDF++L L R  + F F+ G G+A++
Sbjct:  327 PAQRKGKNSVAM-MSRLFDMSCDETLCSADSFCVNDYAWGGSR---------------------------CHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNS---YQAFQVTLEFRAEAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGIAII 460          

BLAST of PREDICTED: pikachurin vs. SwissProt
Match: gi|221271989|sp|B4F785.1|EGFLA_RAT (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor)

HSP 1 Score: 202.601 bits (514), Expect = 1.863e-54
Identity = 135/446 (30.27%), Postives = 209/446 (46.86%), Query Frame = 0

Query:  128 ITSDEAEPNLAPDPPRALPSLSGVAAWPAHSEPKLNQPSGSRNQLPEADGDYKDKVYEIDAFCNLECGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPL-------WNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQGE 566
            +++ E +  LA     +LP  + VA  P  ++ K        ++L +   D  + +   D+FC  +    G              RC C  GK GE C+ D +I  P+F GHSY+    L N+Y   Q+++EFR  +E+G+LL  GES    GD+++L +    +  R +CGTG  I+ +  ++ L  W+ +T++R      LQLN G    G+SQG +S+ITF  P+ LGG  S         TN GF GC++ L +N K  +  P    G  L G D  EC    C    C N G C + +                       D +      +C CPLGF G +CE    + IP F   S  +Y   A   PL       + +FE+  R ++ DG++LY+ + +  S DFL++ ++ G VEF FD G G  ++ + +    G+
Sbjct:  295 VSNSEMDSRLAQPTSASLPETT-VAVPPTPAQRKGKNSVAVMSRLFDMSCD--ETLCSADSFCVNDYAWGGS-------------RCHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGMAIIISETKIKLGAWHSVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGKKIDMRPWP-LGKALNGADVGECSSGICDEASCINGGTCAAIKA----------------------DSY------ICLCPLGFRGRHCEDAFTLTIPQFR-ESLRSY--AATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYSYDTS--SKDFLSIIMAAGHVEFRFDCGSGTGVLRSEDTLTLGQ 690          

HSP 2 Score: 129.798 bits (325), Expect = 3.818e-30
Identity = 108/404 (26.73%), Postives = 192/404 (47.52%), Query Frame = 0

Query:  194 CGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRF--SGHSYLALP---TLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSG----FHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV--EVKIPAFNGSSHLTYFG---LADSSPLWNDFEVVLRSEAEDGLVLYNGNN-NDGSGDFLALFLSRGFVEFAFDNGDGVA--LVSNPEPECQGEQILALKPRSSGSLV 580
            C   G C   + D  +    CLCP G  G  C     ++ P+F  S  SY A P      +  S  +  + FRP S +GVLL + +++  + D+L++I+  GH+E R DCG+G G++R+ D + L +W+ L V R      LQ+++    EG ++G F++I     + +GG  ++ +    +  NSG    F G I+++ +N++  +       G  +      E     C   PC + G C                      +++ Y+         C CPLGF G  C++ +   ++IP F G S+LTY     L   S   ++  +  ++ A+DGL+L+ G++    + DF++L L  G + F+++ G GVA  +V+    + +  ++ A++   SG + 
Sbjct:  570 CINGGTCAAIKADSYI----CLCPLGFRGRHCEDAFTLTIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYSYDTS--SKDFLSIIMAAGHVEFRFDCGSGTGVLRSEDTLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDD----VKKNSGILHPFSGSIQKIILNDRTIHVRHDFTSGVNV------ENAAHPCVGAPCAHGGSC--------------------RPRKEGYE---------CDCPLGFEGLNCQKAITEAIEIPQFIGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKIT 928          

BLAST of PREDICTED: pikachurin vs. SwissProt
Match: gi|158705944|sp|Q63HQ2.2|EGFLA_HUMAN (RecName: Full=Pikachurin; AltName: Full=Agrin-like protein; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor)

HSP 1 Score: 199.904 bits (507), Expect = 1.831e-53
Identity = 120/360 (33.33%), Postives = 178/360 (49.44%), Query Frame = 0

Query:  213 RCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPL-------WNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQG 565
            RC C  GK GE C+ D  I  P+F GHSY+    L N+Y   Q+++EFR  +E+G+LL  GE+    GD+++L I    ++ R +CGTG  I+ +  ++ L  W+ + ++R      LQLN G    G+SQG +S+ITF  P+ LGG  S         TN GF GC++ L +N +  +  P    G  L G D  EC    C    C + G C + +                       D +      +C CPLGF G +CE    + IP F   S  +Y   A   PL       + +FE+  R ++ DG++LY  + + GS DFL++ L+ G VEF FD G G  ++ + +P   G
Sbjct:  368 RCQCTLGKGGESCSEDIVIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYCGENEHGRGDFMSLAIIRRSLQFRFNCGTGVAIIVSETKIKLGGWHTVMLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGRRIDMRPWP-LGKALSGADVGECSSGICDEASCIHGGTCTAIKA----------------------DSY------ICLCPLGFKGRHCEDAFTLTIPQFR-ESLRSY--AATPWPLEPQHYLSFMEFEITFRPDSGDGVLLY--SYDTGSKDFLSINLAGGHVEFRFDCGSGTGVLRSEDPLTLG 693          

HSP 2 Score: 126.716 bits (317), Expect = 3.266e-29
Identity = 104/404 (25.74%), Postives = 186/404 (46.04%), Query Frame = 0

Query:  198 GECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRF--SGHSYLALP---TLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV----------EVKIPAFNGSSHLTYFG---LADSSPLWNDFEVVLRSEAEDGLVLYNGNN-NDGSGDFLALFLSRGFVEFAFDNGDGVA--LVSNPEPECQGEQILALKPRSSGSLV 580
            G C   + D  +    CLCP G  G  C     ++ P+F  S  SY A P      +  S ++  + FRP S +GVLL + ++   + D+L++ +  GH+E R DCG+G G++R+ D + L  W+ L V R      LQ+++    EG ++G F++I     + +GG  ++ +          F G I+++ +N++  + +     G  +      E     C   PC + G C                      +++ YD         C CPLGF G +C++             ++IP F G S+LTY     L   S   ++  +  ++ A+DGL+L+ G++    + DF++L L  G + F+++ G GVA  +V+    + +  ++ A++   SG + 
Sbjct:  578 GTCTAIKADSYI----CLCPLGFKGRHCEDAFTLTIPQFRESLRSYAATPWPLEPQHYLSFMEFEITFRPDSGDGVLLYSYDTG--SKDFLSINLAGGHVEFRFDCGSGTGVLRSEDPLTLGNWHELRVSRTAKNGILQVDKQKIVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGVLKPFSGSIQKIILNDRTIHVKHDFTSGVNV------ENAAHPCVRAPCAHGGSC--------------------RPRKEGYD---------CDCPLGFEGLHCQKECGNYCLNTIIEAIEIPQFIGRSYLTYDNPDILKRVSGSRSNVFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKIT 940          

BLAST of PREDICTED: pikachurin vs. SwissProt
Match: gi|218563482|sp|A2ASQ1.1|AGRIN_MOUSE (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22)

HSP 1 Score: 166.007 bits (419), Expect = 6.942e-42
Identity = 125/375 (33.33%), Postives = 172/375 (45.87%), Query Frame = 0

Query:  214 CLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEE----GRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNS---GFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGV------------CQCPLGFSGEYCEQPVEVK-----IPAFNGSSHLTYFGLADSSPLWND---FEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEP 561
            C C  G+ G  C   +  S P F GHS+LA PTL  AY  L+L++EFR +  EG+LL  G +     D+LAL + DGH++ R D G+GP ++ +   V   RW+RL + RH    W Q    V  E    G S      +     + +GG          LD  S   G  GCIR L+INN+    E S+ Q   +      EC +  CS  PC    +C + + G        G FG   + E+     N P  G             C CPLG SG +CE  +E       +  FNG S+L   GL        +    E+V  +    GL+LYNG   DG GDF++L L    +EF +D G G A++ + EP
Sbjct: 1237 CSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTL-RAYHTLRLALEFRALETEGLLLYNGNARGK--DFLALALLDGHVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRH----WRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPE-EQVATVLDRTSVGIGLKGCIRMLDINNQ--QLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPN-PCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEP 1600          

HSP 2 Score: 108.227 bits (269), Expect = 4.509e-23
Identity = 98/382 (25.65%), Postives = 160/382 (41.88%), Query Frame = 0

Query:  213 RCLCPHGKYGEKC--ALDRYISSP---RFSGHSYLALPTLTNAYSDL----QLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEG---VHEEGRSQGL-FSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSI---PCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV------EVKIPAFNGSSHLTYFGLADSSPLWND----------------------FEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNG 550
            +C CP G+ G  C   L+   S P    F+G SYL L  L     DL     L + F      G+LL  G+  D  GD+++L + + H+E R D G G  I+R+++ + L  W R+ + R+     LQ+ +G   + E  +S+ +  + +   +P+ +GG   F          SGF G I+ + +            Q   L  +D        C+     PC N G C+                     +E  Y+         C CP GFSG +CE+ +      +++  AF+G +++ Y      S L N+                      FE+ LR+EA  GLVL+ G   +   D++AL +  G ++ ++D G
Sbjct: 1494 KCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQLVSLRGHQL-----LTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCI--------------------PREATYE---------CLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGE-RADYMALAIVDGHLQLSYDLG 1840          

BLAST of PREDICTED: pikachurin vs. SwissProt
Match: gi|399021|sp|P25304.2|AGRIN_RAT (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22)

HSP 1 Score: 161.384 bits (407), Expect = 1.935e-40
Identity = 122/375 (32.53%), Postives = 171/375 (45.60%), Query Frame = 0

Query:  214 CLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEE----GRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNS---GFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGV------------CQCPLGFSGEYCEQPVEVK-----IPAFNGSSHLTYFGLADSSPLWND---FEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEP 561
            C C  G+ G  C   +  S P F GHS+LA PTL  AY  L+L++EFR +  EG+LL  G +     D+LAL + DG ++ R D G+GP ++ +   V   RW+RL + RH    W Q    V  E    G S      +     + +GG          LD  S   G  GCIR L+INN+    E S+ Q   +      EC +  C   PC    +C + + G        G FG   + E+     N P  G             C+CPLG SG +C+  +E       +  FNG S+L   GL        +    E+V  +    GL+LYNG   DG GDF++L L    +EF +D G G A++ + EP
Sbjct: 1246 CSCTAGRGGSVCEKVQPPSMPAFKGHSFLAFPTL-RAYHTLRLALEFRALETEGLLLYNGNARGK--DFLALALLDGRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRH----WRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPE-EQVAMVLDRTSVGVGLKGCIRMLDINNQ--QLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPN-PCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEP 1609          

HSP 2 Score: 105.531 bits (262), Expect = 2.811e-22
Identity = 97/382 (25.39%), Postives = 161/382 (42.15%), Query Frame = 0

Query:  213 RCLCPHGKYGEKC--ALDRYISSP---RFSGHSYLALPTLTNAYSDL----QLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEG---VHEEGRSQGL-FSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSI---PCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV------EVKIPAFNGSSHLTYFGLADSSPLWND----------------------FEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNG 550
            +C CP G+ G  C   L+   S P    F+G SYL L  L     DL     L + F      G+LL  G+  D  GD+++L + + H+E   D G G  ++R+++ + L  W R+ + R+     LQ+ +G   + E  +S+ +  + +   +P+ +GG   F         +SGF G I+ + +            Q   L  +D     +  C+     PC N G CV                     +E  Y+         C CP GFSG +CE+ +      +++  AF+G +++ Y      S L N+                      FE+ LR+EA  GLVL+ G   +   D++AL +  G ++ ++D G
Sbjct: 1503 KCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFSGVIQLVSLRGHQL-----LTQEHVLRAVDVSPFADHPCTQALGNPCLNGGSCV--------------------PREATYE---------CLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEYLNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKAAE-RADYMALAIVDGHLQLSYDLG 1849          

HSP 3 Score: 56.6102 bits (135), Expect = 5.597e-7
Identity = 38/142 (26.76%), Postives = 68/142 (47.89%), Query Frame = 0

Query:  249 NAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDT--NSGFHGCIRRLEINNK 388
             A       +  R  + +G++L  G++A+   DY+AL I DGH++L  D G+ P ++R+  +V+ +RW R+   R      LQ+       G S    +++     + LGG       G  L     +GF GC+R + + ++
Sbjct: 1800 KALQSNHFELSLRTEATQGLVLWIGKAAER-ADYMALAIVDGHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKL-PVGQALPKAYGTGFVGCLRDVVVGHR 1939          

BLAST of PREDICTED: pikachurin vs. SwissProt
Match: gi|460018313|sp|P31696.3|AGRIN_CHICK (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22; Flags: Precursor)

HSP 1 Score: 157.147 bits (396), Expect = 5.537e-39
Identity = 112/389 (28.79%), Postives = 176/389 (45.24%), Query Frame = 0

Query:  214 CLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGT--GSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDD-----FNQPLL------GVCQCPLGFSGEYCEQPVEVK-----IPAFNGSSHLTYFGLAD--SSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQGEQILALKPRSSGSLVRR 582
            C CP GK G  C        P F G SYLA   +  AY  +++++EFR     G+LL  G+  +   D+++L +  G +ELR + G+G G++ ++ +V   +W++L V R+     L + +G H  G S      +     + +GG                 G  G IR L++NN++Y+    E+  D L+G    EC  D C   PC +   C   +      +      G   + E+   D      +   L       +C CP+G  GE+CE+  E       +P FNG S+L   GL     +      EVV  +++  G++ YNG   DG GDF++L L  G++E+ +D G G A++ + EP      I  L  RS    V R
Sbjct: 1371 CRCPAGKGGAVCEKPIRYFIPSFGGKSYLAF-KMMKAYHTVRIAMEFRATELSGLLLYNGQ--NRGKDFISLALVGGFVELRFNTGSGTGVITSKVRVEPGKWHQLVVNRNRRSGMLAV-DGEHVSGESPTGTDGLNLDTDLFVGGAPEDQMAVVAERTAATVGLKGSIRLLDVNNQMYDLR--EKGSDVLYGSGVGECGNDPCHPNPCHHGASCHVKEAEMFHCECLHSYTGPTCADERNPCDPTPCHISATCLVLPEGGAMCACPMGREGEFCERVTEQDHTMPFLPEFNGFSYLELNGLQTLFLTCRQMSMEVVFLAKSPSGMIFYNGQKTDGKGDFVSLALHDGYLEYRYDLGKGAAVLRSKEPVPLNTWISVLLERSGRKGVMR 1753          

HSP 2 Score: 114.005 bits (284), Expect = 5.805e-25
Identity = 93/390 (23.85%), Postives = 161/390 (41.28%), Query Frame = 0

Query:  214 CLCPHGKYGEKCALDRYISS-------PRFSGHSYL---ALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRS----QGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV------EVKIPAFNGSSHLTYFGLADSSPLWND----------------------FEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEP 561
            C CP G+ GE C  +R           P F+G SYL    L TL      + + V F   S  G++   G+  D  GD+++L + DG++E R D G G  ++R+++ V L+ W  + + R      +++N G    G S    +   + +   +P  +GG   F         ++ F+G ++R+ I       E   R    +       C +      PCQN G C                    + + + Y+         C C  GFSG +CE+ +      + +  AF+G +++ Y      S L N+                      FE+ +++EA  GL+L++G   + S D++AL +  GFV+  +D G    ++ +  P
Sbjct: 1628 CACPMGREGEFC--ERVTEQDHTMPFLPEFNGFSYLELNGLQTLFLTCRQMSMEVVFLAKSPSGMIFYNGQKTDGKGDFVSLALHDGYLEYRYDLGKGAAVLRSKEPVPLNTWISVLLERSGRKGVMRINNGERVMGESPKSRKVPHAFLNLKEPFYVGGAPDFSKLARAAAISTSFYGAVQRISIKGVPLLKEQHIRSAVEISTFRAHPCTQ---KPNPCQNGGTC--------------------SPRLESYE---------CACQRGFSGAHCEKVIIEKAAGDAEAIAFDGRTYMEYHNAVTKSHLSNEIPAPDALDYPAEPSEKALQSNHFELSIKTEATQGLILWSGKGLERS-DYIALAIVDGFVQMMYDLGSKPVVLRSTVP 1982          

BLAST of PREDICTED: pikachurin vs. SwissProt
Match: gi|460018339|sp|O00468.5|AGRIN_HUMAN (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22; Flags: Precursor)

HSP 1 Score: 146.747 bits (369), Expect = 1.288e-35
Identity = 113/370 (30.54%), Postives = 163/370 (44.05%), Query Frame = 0

Query:  214 CLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDT--NSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGV------------CQCPLGFSGEYCEQPVEVK-----IPAFNGSSHLTYFGLADSSPLWND---FEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEP 561
            C CP G+ G  C        P F G S+LA PTL  AY  L+L++EFR +  +G+LL  G +     D+LAL + DG ++LR D G+GP ++ +   V   +W+RL + RH     L ++      G S      +     + +GG    +       T   +G  GCIR L++NN+    E     G    G    EC +  C   PC     C + + G        G  G   + E+     N P  G             C+CPLG  G +C+           +  FNG SHL   GL   +    +    EVV  +    GL+LYNG   DG GDF++L L    +EF +D G G A++ + EP
Sbjct: 1355 CSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTL-RAYHTLRLALEFRALEPQGLLLYNGNARGK--DFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQ--RLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPN-PCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREP 1718          

HSP 2 Score: 110.923 bits (276), Expect = 6.243e-24
Identity = 104/380 (27.37%), Postives = 164/380 (43.16%), Query Frame = 0

Query:  213 RCLCPHGKYGEKC--ALDRYISSP---RFSGHSYLALPTLTNAYSDL----QLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEG---VHEEGRSQGL-FSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNK-IYNFEPSERQGD-TLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV------EVKIPAFNGSSHLTYFGLADSSPLWND---------------------FEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNG 550
            +C CP G+ G  C  A  +  S P    F+G S+L L  L     DL     L V F      G+LL  G+  D  GD+++L +RD  +E R D G G  ++R+R+ V L  W R+++ R+     L++ +G   + E  +S+ +  + +   +P+ +GG   F         +SGF G I+ + +  + +   E   RQ D T F       P    S  PC N   CV                     +E  Y         VC CP GFSG +CE+ +      +V   AF+G + + Y      S L N+                     FE+ LR+EA  GLVL++G   +   D++AL +  G ++ +++ G
Sbjct: 1612 QCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGH----PCTRASGHPCLNGASCV--------------------PREAAY---------VCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESELANEIPVPETLDSGALHEKALQSNHFELSLRTEATQGLVLWSGKATE-RADYVALAIVDGHLQLSYNLG 1957          

HSP 3 Score: 62.003 bits (149), Expect = 1.173e-8
Identity = 47/147 (31.97%), Postives = 61/147 (41.50%), Query Frame = 0

Query:  415 CSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEP 561
            C S PC + G C        ++   GG F                    C CP G  G  CE+ +   +PAF G S L +  L     L    E   R+    GL+LYNGN      DFLAL L  G V+  FD G G A++++  P
Sbjct: 1333 CDSQPCFHGGTC--------QDWALGGGF-------------------TCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALE--FRALEPQGLLLYNGNAR--GKDFLALALLDGRVQLRFDTGSGPAVLTSAVP 1448          

BLAST of PREDICTED: pikachurin vs. SwissProt
Match: gi|2494281|sp|Q90404.1|AGRIN_DIPOM (RecName: Full=Agrin)

HSP 1 Score: 144.821 bits (364), Expect = 5.771e-35
Identity = 107/375 (28.53%), Postives = 168/375 (44.80%), Query Frame = 0

Query:  210 IKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNT--GHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQE-------YDDFNQPLL----GVCQCPLGFSGEYCEQPVEVK-------IPAFNGSSHLTYFGLAD-SSPLWNDF--EVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEP 561
            +   C CP G+ G  C        P F G SYLA  T+  AY  +++S+EFR  + +G+ L+          +      +G++ELR + G   G++ ++  +    W+ +   R+     L ++   H  G S      +     + LGGT     T          G  GCIR L++NN IY+ +  ER  D L+G    EC  + CS  PC+N G C   +      +  G   G   + +         +   N  +L      C+CP+G  GE CE+  E +       IP FNG S+L   G+    S L      EV+  ++  +G++ YNG   DG GDF++L L  G++EF +D G G A++ +  P
Sbjct:  628 VSYTCSCPAGRGGAVCERTIVYFIPEFGGRSYLAFKTM-KAYYTVRISMEFRASNLDGLPLVQWTEKGKGLHFYR--PSEGYVELRFNHGVWDGVITSKTLIKPGNWHHVVGNRNRRSGMLSVDGEPHLIGESPPGTDGLNLDTDLFLGGTPEDEMTLVTERTTATKGLQGCIRLLDVNNLIYDLQ--ERSNDVLYGSGVGECGNNPCSPNPCKNRGKCHMKEAEMFHCESVGEFSGPTCADKHNPCDPNPCHQSANCMVLPEGGSKCECPMGREGELCERVSEAEQDQGKAFIPEFNGLSYLEMNGIHTFVSDLLQKLSMEVIFLAKDPNGMIFYNGQKTDGRGDFVSLNLRDGYLEFKYDLGKGAAVLRSKAP 997          

HSP 2 Score: 124.405 bits (311), Expect = 2.199e-28
Identity = 98/366 (26.78%), Postives = 167/366 (45.63%), Query Frame = 0

Query:  213 RCLCPHGKYGEKCALDRYISS---------PRFSGHSYLALPTLTNAYSDL--QLSVE--FRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRS----QGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSI--PCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIP------AFNGSSHLTYFGL---ADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNG 550
            +C CP G+ GE C  +R   +         P F+G SYL +  +    SDL  +LS+E  F      G++   G+  D  GD+++L +RDG++E + D G G  ++R++  + L+ WN +TV R+     +++N+     G S    +   + +   +   +GG   F          SGF G I++L + +      P  ++ +    M+        C+    PCQN G+C                    + + +EYD         C C  GFSG  CE+ +E K        AFNG + + Y      ++ +   N FE+ +++EA  GL+L++G   + S D++AL +  GFV+  +D G
Sbjct:  889 KCECPMGREGELC--ERVSEAEQDQGKAFIPEFNGLSYLEMNGIHTFVSDLLQKLSMEVIFLAKDPNGMIFYNGQKTDGRGDFVSLNLRDGYLEFKYDLGKGAAVLRSKAPIPLNVWNVVTVERNGRKGLMKINKDELVSGESPKSRKAPHTALNLKEAFYVGGAPDFNKFARAAGIISGFTGAIQKLSLKSI-----PLLKKENIRNAMEISNFRWHACTKTRNPCQNGGVC--------------------SPRLREYD---------CMCQRGFSGPQCEKALEEKSASGSESVAFNGRTFIEYHNTVTRSEKAVQVNYFEMSIKTEATKGLILWSGKIAEKS-DYIALAVVDGFVQMTYDLG 1217          

BLAST of PREDICTED: pikachurin vs. SwissProt
Match: gi|1172451|sp|Q05793.1|PGBM_MOUSE (RecName: Full=Basement membrane-specific heparan sulfate proteoglycan core protein; Short=HSPG; Contains: RecName: Full=Endorepellin; Contains: RecName: Full=LG3 peptide; Flags: Precursor)

HSP 1 Score: 130.568 bits (327), Expect = 3.054e-30
Identity = 117/407 (28.75%), Postives = 186/407 (45.70%), Query Frame = 0

Query:  214 CLCPHGKYGEKCALDRYISSPRFSGH-SYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGV----HEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLL-------GVCQCPLGFSGEYCEQPVEVKI------PAFNGSS-----HLTYF------GLADSS--------PLWNDFEVVLRSEAEDGLVLYNGNNNDGS--GDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQGE--QILALKPRSSGSL 579
            C C  G+ G +C     +++P  SG  SYLALP LTN + +L+L VEF+P+   G+LL +G  +    D+++L +  GH+E R + G+G  ++R+ + + L RW+R++  R +    L+++ G        G+SQGL         + LGG           + ++ FHGC+  + +N K  +   S   G    G   D  P   C   PC+N   C+ A     +     G  G L   E+     ++P L         C C  GFSG  C+Q     +      P  +G +     +  YF      GL  +S        P   +FEV  R+   DGL+L+ G   + S   DF++L L  G + F++  G G A + + +P   GE  +I AL+    GS+
Sbjct: 3227 CRCHLGRSGVRCEEGVTVTTPSMSGAGSYLALPALTNTHHELRLDVEFKPLEPNGILLFSGGKSGPVEDFVSLAMVGGHLEFRYELGSGLAVLRSHEPLALGRWHRVSAERLNKDGSLRVDGGRPVLRSSPGKSQGL----NLHTLLYLGGVEPSVQLSPATNMSAHFHGCVGEVSVNGKRLDLTYS-FLGSQGVGQCYDSSP---CERQPCRNGATCMPAGEYEFQCLCQDGFKGDLCEHEENPCQLHEPCLNGGTCRGARCLCLPGFSGPRCQQGAGYGVVESDWHPEGSGGNDAPGQYGAYFYDNGFLGLPGNSFSRSLPEVPETIEFEV--RTSTADGLLLWQGVVREASRSKDFISLGLQDGHLVFSYQLGSGEARLVSGDPINDGEWHRITALREGQRGSI 3623          

HSP 2 Score: 110.153 bits (274), Expect = 1.075e-23
Identity = 98/365 (26.85%), Postives = 163/365 (44.66%), Query Frame = 0

Query:  239 HSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGES------ADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGG---TGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGG-------GGEEDVFGGAFGTLTSQEQEYDD---FNQP--LLGVCQCPLGFSGEYCEQPVEVKIPAFNGS-SHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQGE--QILALKPRSSGSL 579
            +S+L LPT+ +AY   ++ + FRP S +G+LL  G+       A+   D+++  +  G  E R D G+G   +R    + L +++ +T+ R      L +       G SQG F  +   + + LGG    G+    G     +SGF GC+R L I  +   F         L       CP  TC   PCQN G C  ++          G            T+    + D    N+P      C+C LG SG  CE+ V V  P+ +G+ S+L    L ++       +V  +    +G++L++G  +    DF++L +  G +EF ++ G G+A++ + EP   G   ++ A +    GSL
Sbjct: 2992 YSFLPLPTIKDAYRKFEIKITFRPDSADGMLLYNGQKRSPTNLANRQPDFISFGLVGGRPEFRFDAGSGMATIRHPTPLALGQFHTVTLLRSLTQGSLIVGNLAPVNGTSQGKFQGLDLNEELYLGGYPDYGAIPKAG----LSSGFVGCVRELRIQGEEIVF-----HDVNLTTHGISHCP--TCQDRPCQNGGQCQDSESSSYTCVCPAGFTAAAVNIRKPCTATPSLWADATCVNRPDGRGYTCRCHLGRSGVRCEEGVTVTTPSMSGAGSYLALPALTNTHHELR-LDVEFKPLEPNGILLFSGGKSGPVEDFVSLAMVGGHLEFRYELGSGLAVLRSHEPLALGRWHRVSAERLNKDGSL 3344          

BLAST of PREDICTED: pikachurin vs. nr
Match: gi|1325265600|ref|XP_023337213.1| (pikachurin-like [Eurytemora affinis])

HSP 1 Score: 419.468 bits (1077), Expect = 4.720e-133
Identity = 234/522 (44.83%), Postives = 303/522 (58.05%), Query Frame = 0

Query:   70 VAIQIEVDIDPPPHQPNDPSDAELEKLGLPTHHVHY-----STRTAPGESGSPQ------PGSESVIRPITSDEAE------PNLAP---------------DPPRALPSLSGVAAWPAHSEPKLNQPSGSRNQLPEADGDY-KDKVYEIDAFCNLECGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDD--FNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALV 556
            +AIQIEVDI+PP HQ  D S+ +L KLGLPTHHVHY     + + A   SG P       P    V   + ++E +       N AP               DPPRALP+++         EP L             D  + KD +Y + AFC L CG  G C   R D   I  RC+CP G  G +CA    IS P  SG  +++ PTL NAYSDL LS+EF+P +  G+LLLTGE+ DMTGDYLAL+++DG+IELRLDCGTGPG+V+T  QVHL+ WN ++VFRHDWGVW+QLN G  EEGRSQGLFSRITF QPV +GG+G  RN  +F+  + G  GC+R LEIN+  Y+F P    GD++ G+DT  C    CS+  C                       GT   Q   +     NQ   G C CPLG SG+ C++   +++P F G S L + GL D++ +W + E+V       GL+LYNGN NDG GDF+A+FL++GFVEFAFD G+G+A+ 
Sbjct:   93 IAIQIEVDIEPPLHQDQDLSEEQLHKLGLPTHHVHYRAGQPAHQAARQPSGQPAGTELLAPADIQVSYTVANEETQIKKQDGENFAPPHYSRDESQAPVEYLDPPRALPAVA---------EPYL------------VDSAFLKDNIYTVPAFCGLNCGT-GTCHTERVDTEWIS-RCICPAGWGGAQCAEHIDISIPELSGTGFISFPTLQNAYSDLHLSLEFKPTAWTGLLLLTGETEDMTGDYLALVLKDGYIELRLDCGTGPGVVKTSSQVHLNAWNHVSVFRHDWGVWVQLNGGKREEGRSQGLFSRITFNQPVYIGGSGEMRNVKNFVGVDQGLQGCVRHLEINDLSYSFNPVSTGGDSIGGIDTGVCKTPGCSNSMCGQ---------------------GTCRDQSSPFSSNPANQSRQG-CDCPLGSSGDRCQRQEHIQMPRFVGDSFLRHAGLGDAALIWLEMEIVFNPSQLQGLILYNGNRNDGKGDFVAVFLNQGFVEFAFDLGNGIAIA 569          

HSP 2 Score: 147.132 bits (370), Expect = 2.147e-33
Identity = 102/361 (28.25%), Postives = 166/361 (45.98%), Query Frame = 0

Query:  202 MTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNA-YSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV--EVKIPAFNGSSHL------TYFGLADSSPLWNDFEVVLRSEAEDGLVLY-NGNNNDGSGDFLALFLSRGFVEFAFDNGDG 552
             + +  N  ++ C CP G  G++C    +I  PRF G S+L    L +A    L++ + F P   +G++L  G   D  GD++A+ +  G +E   D G G  I R+  +V +  W+ LT+ R    V+L L+    E   S G F++++ +Q + LGG   +     +L   + FHGCI++L +N       P +  G  L G++ + C   +C   PC   GIC                           D F+      CQCPLG+ GE C  P      IP F G+S+L       Y  L  ++   N   + LR  +  GL+++  G  ++ + D+L L +  G+++F F+ G G
Sbjct:  465 FSSNPANQSRQGCDCPLGSSGDRCQRQEHIQMPRFVGDSFLRHAGLGDAALIWLEMEIVFNPSQLQGLILYNGNRNDGKGDFVAVFLNQGFVEFAFDLGNGIAIARSGSKVKIGSWHSLTISRTGREVYLSLDGEEGETVTSPGGFTQLSLSQNLWLGGVPEYGILSAYLPITTAFHGCIQKLVVNGL-----PIQLVGSALSGLNVESCVH-SCQGSPC-GAGIC-----------------------SPNLDTFS------CQCPLGWGGESCSTPTPDSSPIPGFTGNSYLFFNNQDIYQNLVGTA---NSINIRLRVTSAHGLLMWVGGEKSEPTPDYLLLGVHNGYLQFVFNVGSG 786          

BLAST of PREDICTED: pikachurin vs. nr
Match: gi|1228002413|ref|XP_021933090.1| (pikachurin isoform X2 [Zootermopsis nevadensis] >gi|1228002415|ref|XP_021933091.1| pikachurin isoform X2 [Zootermopsis nevadensis] >gi|1228002417|ref|XP_021933092.1| pikachurin isoform X2 [Zootermopsis nevadensis] >gi|1228002419|ref|XP_021933093.1| pikachurin isoform X2 [Zootermopsis nevadensis])

HSP 1 Score: 338.961 bits (868), Expect = 1.302e-103
Identity = 169/378 (44.71%), Postives = 232/378 (61.38%), Query Frame = 0

Query:  190 CNLECGVEGECFMTRDDKNVI-KKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQGE 566
            C L+CGV G C M   D +   ++RC CP G+ G  C  D  I SPRF+G  +LA P L  AY  +QL +EFRP + +G+L LTGE  DM GD++AL++  G +E R DCG+G G+VR+ + V L++WNRLT++RH W  W+QLN G H EGRS+GLFSRITF +P+ +GG G+       L T  G  GCIR LE+N+  Y+F  +   GD + G D +EC  D CS +PCQ+ G C                   L S E            VC CPLGF+G+ CE  V++++P+FNGSS+L Y GL  S+  W D ++ L+  A+DG++LYNG+ +DG GDF+A+++S G +EF FD G G A + + EP   GE
Sbjct:   87 CLLDCGVGGSCAMDAVDSDRPGEQRCQCPLGRGGRHCQADVEIRSPRFTGQGWLAFPALRAAYKHVQLELEFRPEAWDGILFLTGERDDMAGDFMALLLHQGFVEFRFDCGSGLGVVRSEETVLLNQWNRLTLYRHRWDAWIQLNNGKHVEGRSKGLFSRITFREPLFVGGPGNTTGLADKLPTEDGLKGCIRHLEVNDHAYSFNLAP-LGDAVKGFDVEECSADRCSKVPCQHGGKC-------------------LISGET----------AVCLCPLGFTGDLCETRVDLQVPSFNGSSYLRYPGLGGSALSWLDLQLTLKPSAQDGVILYNGHRSDGVGDFMAVYMSDGHLEFTFDLGTGAATIRSGEPVTLGE 434          

HSP 2 Score: 150.214 bits (378), Expect = 1.217e-34
Identity = 118/428 (27.57%), Postives = 192/428 (44.86%), Query Frame = 0

Query:  145 LPSLSGVAAWPAHSEPKLNQPSGSRNQLPEADGDYKDKVYEI--DAFCNLECGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLT-NAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV------EVKIPAFNGSSHLTYFGLADSSPLWN------DFEVVLRSEAEDGLVLYNGNNN-DGSGDFLALFLSRGFVEFAFDNGDGVALV 556
            LP+  G+     H E  +N  + S N  P  D      V E   D    + C   G+C ++ +        CLCP G  G+ C     +  P F+G SYL  P L  +A S L L +  +P +++GV+L  G  +D  GD++A+ + DGH+E   D GTG   +R+ + V L  W+ L + R      LQ++  V  +  + G F++++  Q + +GG  +F      +   + F GCI+++ INN     +P +     L G++ D CP   C + PC + G CV       E D F                        C+C  G     C+Q        ++ IP F G S+L Y   +D   +        +  +  R+ +  GL+L++G  N   +GDFL L ++ G++ F F+ G G  ++
Sbjct:  268 LPTEDGLKGCIRHLE--VNDHAYSFNLAPLGDAVKGFDVEECSADRCSKVPCQHGGKCLISGE-----TAVCLCPLGFTGDLCETRVDLQVPSFNGSSYLRYPGLGGSALSWLDLQLTLKPSAQDGVILYNGHRSDGVGDFMAVYMSDGHLEFTFDLGTGAATIRSGEPVTLGEWHELRLSRTGRLAILQVDNMVPTQAMAPGAFTQLSLPQNLYIGGVPNFDMVSPKVKVRTSFVGCIQKVVINN-----QPLQILAAALGGVNVDNCPH-PCVTRPCGDEGHCVP------EMDYF-----------------------TCECAQGHHDVQCQQHAAPTILQDIPIPRFAGDSYLHY---SDPDTMRRIISYKININMRFRTSSSVGLLLWSGRRNMSAAGDFLGLGIADGYLHFRFNLGSGEVII 650          

BLAST of PREDICTED: pikachurin vs. nr
Match: gi|1228002411|ref|XP_021933089.1| (pikachurin isoform X1 [Zootermopsis nevadensis])

HSP 1 Score: 338.191 bits (866), Expect = 3.867e-103
Identity = 169/378 (44.71%), Postives = 232/378 (61.38%), Query Frame = 0

Query:  190 CNLECGVEGECFMTRDDKNVI-KKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQGE 566
            C L+CGV G C M   D +   ++RC CP G+ G  C  D  I SPRF+G  +LA P L  AY  +QL +EFRP + +G+L LTGE  DM GD++AL++  G +E R DCG+G G+VR+ + V L++WNRLT++RH W  W+QLN G H EGRS+GLFSRITF +P+ +GG G+       L T  G  GCIR LE+N+  Y+F  +   GD + G D +EC  D CS +PCQ+ G C                   L S E            VC CPLGF+G+ CE  V++++P+FNGSS+L Y GL  S+  W D ++ L+  A+DG++LYNG+ +DG GDF+A+++S G +EF FD G G A + + EP   GE
Sbjct:  108 CLLDCGVGGSCAMDAVDSDRPGEQRCQCPLGRGGRHCQADVEIRSPRFTGQGWLAFPALRAAYKHVQLELEFRPEAWDGILFLTGERDDMAGDFMALLLHQGFVEFRFDCGSGLGVVRSEETVLLNQWNRLTLYRHRWDAWIQLNNGKHVEGRSKGLFSRITFREPLFVGGPGNTTGLADKLPTEDGLKGCIRHLEVNDHAYSFNLAPL-GDAVKGFDVEECSADRCSKVPCQHGGKC-------------------LISGET----------AVCLCPLGFTGDLCETRVDLQVPSFNGSSYLRYPGLGGSALSWLDLQLTLKPSAQDGVILYNGHRSDGVGDFMAVYMSDGHLEFTFDLGTGAATIRSGEPVTLGE 455          

HSP 2 Score: 149.828 bits (377), Expect = 1.969e-34
Identity = 118/428 (27.57%), Postives = 192/428 (44.86%), Query Frame = 0

Query:  145 LPSLSGVAAWPAHSEPKLNQPSGSRNQLPEADGDYKDKVYEI--DAFCNLECGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLT-NAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV------EVKIPAFNGSSHLTYFGLADSSPLWN------DFEVVLRSEAEDGLVLYNGNNN-DGSGDFLALFLSRGFVEFAFDNGDGVALV 556
            LP+  G+     H E  +N  + S N  P  D      V E   D    + C   G+C ++ +        CLCP G  G+ C     +  P F+G SYL  P L  +A S L L +  +P +++GV+L  G  +D  GD++A+ + DGH+E   D GTG   +R+ + V L  W+ L + R      LQ++  V  +  + G F++++  Q + +GG  +F      +   + F GCI+++ INN     +P +     L G++ D CP   C + PC + G CV       E D F                        C+C  G     C+Q        ++ IP F G S+L Y   +D   +        +  +  R+ +  GL+L++G  N   +GDFL L ++ G++ F F+ G G  ++
Sbjct:  289 LPTEDGLKGCIRHLE--VNDHAYSFNLAPLGDAVKGFDVEECSADRCSKVPCQHGGKCLISGE-----TAVCLCPLGFTGDLCETRVDLQVPSFNGSSYLRYPGLGGSALSWLDLQLTLKPSAQDGVILYNGHRSDGVGDFMAVYMSDGHLEFTFDLGTGAATIRSGEPVTLGEWHELRLSRTGRLAILQVDNMVPTQAMAPGAFTQLSLPQNLYIGGVPNFDMVSPKVKVRTSFVGCIQKVVINN-----QPLQILAAALGGVNVDNCPH-PCVTRPCGDEGHCVP------EMDYF-----------------------TCECAQGHHDVQCQQHAAPTILQDIPIPRFAGDSYLHY---SDPDTMRRIISYKININMRFRTSSSVGLLLWSGRRNMSAAGDFLGLGIADGYLHFRFNLGSGEVII 671          

BLAST of PREDICTED: pikachurin vs. nr
Match: gi|1069809065|ref|XP_018328441.1| (PREDICTED: pikachurin [Agrilus planipennis])

HSP 1 Score: 338.961 bits (868), Expect = 7.511e-103
Identity = 169/377 (44.83%), Postives = 233/377 (61.80%), Query Frame = 0

Query:  190 CNLECGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQGE 566
            C L+CG  G C +    +   +  CLCP GK G++C  D  I SPRFSG S+LALPTL  AY  +Q+++E RP + +G+  LTGE  DM GD++AL++  G +E R DCG+G GI+R+ + V L++WN +++FRH W  WLQLN G H +GRS+GLFSRITF +P+ +GGTG+  +    L  +SG  GC+R L++N+  Y F   E +G+ L G   +EC  D CS IPCQ+ G C+ ++G                               VC CPLGFSG+ CE  V++ +PAFNGSS+L Y GL D +  W D E+VL+    +GLVLYNG+  DG GDF+AL+L++GFVEF +D G G A+V +      GE
Sbjct:  172 CYLDCGPGGGCVL---KEPYFQPTCLCPLGKGGDRCDKDVEIRSPRFSGSSWLALPTLRGAYKHVQITLELRPEAYDGIFFLTGERDDMQGDFMALLLFQGFVEFRFDCGSGIGILRSEETVLLNQWNHISLFRHRWDAWLQLNNGKHVQGRSKGLFSRITFREPLFIGGTGNITSLSEKLPVSSGIKGCLRNLQVNDNSYKF-ALEPKGEALKGYGVEECTADRCSRIPCQHGGKCLQSEGSA-----------------------------VCLCPLGFSGDLCEIRVDLHVPAFNGSSYLRYRGLGDDTLTWLDLEIVLKPNTPNGLVLYNGHRVDGFGDFMALYLNQGFVEFMYDLGTGFAIVRSDHKVSLGE 515          

HSP 2 Score: 115.931 bits (289), Expect = 2.489e-23
Identity = 96/341 (28.15%), Postives = 149/341 (43.70%), Query Frame = 0

Query:  214 CLCPHGKYGEKCALDRYISSPRFSGHSYLALPTL-TNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFG-----LADSS-PLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAF 547
            CLCP G  G+ C +   +  P F+G SYL    L  +  + L L +  +P +  G++L  G   D  GD++AL +  G +E   D GTG  IVR+  +V L  W+ + V R      L+++  +  E  S G F++++  Q + LGG  +F      +   S F GCI+++ IN +     P     + L G++ D CP   C + PC   G C+                      + EY          CQC  GF  E  +  V    P+F+G     +F      LA +S P   +    L S   DGL+L+    + G   FL+L + +G +   F
Sbjct:  413 CLCPLGFSGDLCEIRVDLHVPAFNGSSYLRYRGLGDDTLTWLDLEIVLKPNTPNGLVLYNGHRVDGFGDFMALYLNQGFVEFMYDLGTGFAIVRSDHKVSLGEWHEIKVSRTGRLAILEVDNQIPAEVMSPGAFTQLSLPQNLFLGGVPNFGIVSLQVRVRSAFTGCIQKVVINGR-----PVSILAEALGGVNVDNCPH-PCVARPCGKAGDCIP---------------------QLEY--------FTCQCKPGFHNE--QLCVSRSPPSFHGKDSFVHFNDTKTLLAFTSDPFSINVRCRLSSTLTDGLLLW---ISSGPEKFLSLGVEQGSLVLRF 713          

BLAST of PREDICTED: pikachurin vs. nr
Match: gi|1101355976|ref|XP_018904138.1| (PREDICTED: pikachurin-like [Bemisia tabaci])

HSP 1 Score: 335.495 bits (859), Expect = 3.511e-101
Identity = 165/379 (43.54%), Postives = 231/379 (60.95%), Query Frame = 0

Query:  190 CNLECGVEGECFMTRDDKNVIK-KRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGIC-VSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQGE 566
            C+L+CG +G C +  D  +    +RC CP+ K G +C  +  +  PRFSG  +LALP L  AY  +Q+ +EFRP S  G+L LTGE  D+ GD++A+++  G IELR DCG+G G+VR+ + V L++WN+LTV+RH W  W+Q N G H +GRS+GLFSRITF +PV +GG G+       L T+ GF GCIR  E+N+ +YNF P + QGD + G D +EC  D CS +PCQ+ G C VS Q                                VC CPLGF G+ CE  +++++P+FNGSS+L Y G+   +  W D ++V++  AEDG++LYNG   DG GDF+A+++S G + F FD G G A V + E    GE
Sbjct:  217 CSLDCGPKGTCIVNSDASSYPGLERCQCPYTKSGAQCKTETEVRWPRFSGQGWLALPPLQAAYKHVQMLIEFRPESWHGILFLTGERDDLAGDFIAILLNQGFIELRFDCGSGVGVVRSDETVKLNQWNKLTVYRHRWNAWIQFNNGKHIQGRSKGLFSRITFREPVFIGGRGNTTGLLDKLPTDYGFRGCIRHFEVNDHVYNFAP-QPQGDVVKGFDVEECKADRCSRMPCQHGGKCMVSGQSA------------------------------VCLCPLGFIGDLCETRLDLQVPSFNGSSYLRYPGMKGRALSWLDMKLVIKPTAEDGVILYNGRRGDGVGDFMAVYISEGRIYFTFDLGTGAATVRSEERVSLGE 564          

HSP 2 Score: 122.865 bits (307), Expect = 1.463e-25
Identity = 88/348 (25.29%), Postives = 157/348 (45.11%), Query Frame = 0

Query:  214 CLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLT-NAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPV-EVKIPAFNGSSHLTYFGLADSSPLWND---FEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALV 556
            CLCP G  G+ C     +  P F+G SYL  P +   A S L + +  +P +E+GV+L  G   D  GD++A+ I +G I    D GTG   VR+ ++V L  W+ + V R      L+++EG      S G F++++    + LGG  +F      +   + F GCI+++ IN++     P +   + L G++ D C +  C + PC ++  CV                               P+    +C   +  E   +   E+   +F G+++  Y        + ND     +  ++ +  GL+L++G ++ G+ DF A+ +S G +   ++ G G   +
Sbjct:  462 CLCPLGFIGDLCETRLDLQVPSFNGSSYLRYPGMKGRALSWLDMKLVIKPTAEDGVILYNGRRGDGVGDFMAVYISEGRIYFTFDLGTGAATVRSEERVSLGEWHEVVVSRTGRLAVLEVDEGPASHELSPGAFTQLSLPLNLYLGGVPNFDMVSPKVKARTSFQGCIQKVVINDR-----PLQIFAEALAGVNVDNC-QHPCLAKPCGDSARCV-------------------------------PIKESYKCECSYQCEDSNKLTGEISGASFAGTNYFHYSDSNIIHRIVNDKLSINMRFKTYSSSGLLLWSGRSDRGN-DFFAVGISGGLLHLRYNLGSGEVFI 771          

BLAST of PREDICTED: pikachurin vs. nr
Match: gi|1133422332|ref|XP_019866819.1| (PREDICTED: pikachurin [Aethina tumida])

HSP 1 Score: 333.183 bits (853), Expect = 9.052e-101
Identity = 162/367 (44.14%), Postives = 228/367 (62.13%), Query Frame = 0

Query:  186 IDAFCNLECGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDG 552
            I+  C L+CG  G C +   D+   + +CLCP GK G+ C  D  I SPRF+G S+LA P L  AY  +QL++E RP + +G+  LTGE  DM GD++AL++  G +E R DCG+G G+VR+ + V L++WN +T++RH W  WLQLN G H +GRS+GLFSRITF +P+ +GG G+    G  L  + G  GCIR LE+N+  Y F   E +G+T  G   +EC  D CS +PCQ+ G C++++                                VC CPLGFSG+ CE  V++++P+FNGSSHL Y GL+D++  W D E++ +  A DGL++YNG+  DG GDF+ L+L+ GFVEFA+D G G
Sbjct:  166 IEKLCPLDCGPGGGCSLGVGDE---QPKCLCPLGKGGKGCEEDMEIRSPRFTGSSWLAFPALRGAYKHVQLALELRPEAYDGIFFLTGERDDMAGDFMALLLHQGFVEFRFDCGSGVGVVRSEETVLLNQWNHITLYRHRWDAWLQLNSGKHVQGRSKGLFSRITFREPLFIGGPGNTTGLGEKLPVSKGLKGCIRHLEVNDHPYKF-ALEPKGETTKGYGIEECTADRCSRVPCQHGGKCLTSETSA-----------------------------VCLCPLGFSGDLCEIRVDLQVPSFNGSSHLRYRGLSDAALTWLDLEIIFKPTAPDGLMMYNGHRLDGMGDFMGLYLNDGFVEFAYDLGTG 499          

HSP 2 Score: 122.094 bits (305), Expect = 2.828e-25
Identity = 98/379 (25.86%), Postives = 173/379 (45.65%), Query Frame = 0

Query:  214 CLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNA-YSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWN------DFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAF---DNGDGVALVSNPEPECQG--EQILALKPRSSGSLV 580
            CLCP G  G+ C +   +  P F+G S+L    L++A  + L L + F+P + +G+++  G   D  GD++ L + DG +E   D GTG  + R+R +V L  W+++ + R      L ++  +  E  S G F++++  Q + LGG  +F      +   + F GC+++++IN ++          + L G + D CP   C + PC  +G CV                        + D F       C+C  G+    C +      PAF+G++  +Y  L D+  L        D  V  +  +E GL+L+       +G +L+L L +G +   +   D+ + + +V N      G   ++ A++  SSG LV
Sbjct:  411 CLCPLGFSGDLCEIRVDLQVPSFNGSSHLRYRGLSDAALTWLDLEIIFKPTAPDGLMMYNGHRLDGMGDFMGLYLNDGFVEFAYDLGTGSTVARSRYRVSLGEWHKVRISRTGRLAILSVDNHLPVEVLSPGAFTQLSLPQNLYLGGVPNFGIVSPQVRVRTAFVGCVQKVQINGRLVAI-----LAEALGGANVDNCPH-PCVARPCGEDGECVP-----------------------QLDYFT------CRCKPGYRDHLCSR----GPPAFHGTN--SYLHLNDAVTLDTISTDPLDVNVRFKLSSESGLLLWAST----TGSYLSLGLEKGALVLRYSVRDSDEEIKVVYNSTTVHDGLWHRVKAVRDGSSGLLV 744          

HSP 3 Score: 79.7221 bits (195), Expect = 7.487e-12
Identity = 42/97 (43.30%), Postives = 57/97 (58.76%), Query Frame = 0

Query:  464 CQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPE 560
            C CPLG  G+ CE+ +E++ P F GSS L +  L  +        + LR EA DG+    G  +D +GDF+AL L +GFVEF FD G GV +V + E
Sbjct:  191 CLCPLGKGGKGCEEDMEIRSPRFTGSSWLAFPALRGAYK-HVQLALELRPEAYDGIFFLTGERDDMAGDFMALLLHQGFVEFRFDCGSGVGVVRSEE 286          

BLAST of PREDICTED: pikachurin vs. nr
Match: gi|1130253104|ref|XP_019767089.1| (PREDICTED: pikachurin-like, partial [Dendroctonus ponderosae])

HSP 1 Score: 323.168 bits (827), Expect = 1.836e-100
Identity = 155/361 (42.94%), Postives = 222/361 (61.50%), Query Frame = 0

Query:  190 CNLECGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNG 550
            C L+CG  G C    D++     +CLCP G+ G++C  D  I SPRF+G S+LA P L  AY  +QLS+E RP + +G+  LTGE  DM GD++AL++  G +E R DCG+G G+VR+ + V L++WN + ++RH W  WLQLN G H +GRS+GLFSRITF +P+ +GG G+       L  + G  GCIR LE+N+ +Y F   +  G+ + G   +EC  D CS +PCQ+ G C++++                                VC CPLGFSG+ CE  V++++P+FNGSSHL Y GLA+S+  W D E+  +    DGL++YNG+  DG+GDF+ L+L+ GF+EFA+D G
Sbjct:  169 CVLDCGPGGGCHAETDEE---PNKCLCPLGRGGDQCERDMEIQSPRFTGSSWLAFPALRGAYKHVQLSLELRPEAYDGIFFLTGERDDMAGDFMALLLHQGFVEFRFDCGSGIGVVRSDETVLLNQWNHIKLYRHRWDAWLQLNNGKHIQGRSKGLFSRITFREPLFIGGPGNTTGLDEKLPVSKGLKGCIRHLEVNDHVYKF-ALDPNGEAIKGYGIEECTADRCSRVPCQHGGKCLTSETSA-----------------------------VCLCPLGFSGDLCEIRVDLQVPSFNGSSHLRYRGLAESALTWLDLEITFKPTLSDGLIIYNGHRMDGTGDFMGLYLNDGFLEFAYDLG 496          

HSP 2 Score: 78.9518 bits (193), Expect = 8.092e-12
Identity = 41/97 (42.27%), Postives = 57/97 (58.76%), Query Frame = 0

Query:  464 CQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPE 560
            C CPLG  G+ CE+ +E++ P F GSS L +  L  +        + LR EA DG+    G  +D +GDF+AL L +GFVEF FD G G+ +V + E
Sbjct:  190 CLCPLGRGGDQCERDMEIQSPRFTGSSWLAFPALRGAYK-HVQLSLELRPEAYDGIFFLTGERDDMAGDFMALLLHQGFVEFRFDCGSGIGVVRSDE 285          

BLAST of PREDICTED: pikachurin vs. nr
Match: gi|1279738015|ref|XP_022913817.1| (pikachurin [Onthophagus taurus])

HSP 1 Score: 332.028 bits (850), Expect = 2.068e-100
Identity = 170/379 (44.85%), Postives = 233/379 (61.48%), Query Frame = 0

Query:  190 CNLECGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNF--EPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQGE 566
            C L+CG  G C ++ +    +   CLCP GK GE+C  D  I SPRF+G S+LA P L  AY  +Q+++E RP S +G+  LTGE  DM GD++AL++  G IE R DCG+G G+VR+ + V L++WN +T++RH W  WLQLN G H +GRS+GLFSRITF +P+ +GGTG+       L  + G  GCIR LE+N+ +Y F  EPS   G+   G   +EC  D CS +PCQ+ G C++++                TS              VC CPLGFSG+ CE  V++++P+FNGSSHL Y GL + +  W D +V L+  AEDGL+LYNG+  +G GDF+AL+L +G V FA+D G GVA  S+      GE
Sbjct:  156 CVLDCGPNGGCVISEESSEPV---CLCPLGKGGERCEKDLEIRSPRFTGSSWLAFPALRGAYKHVQVNLELRPESYDGIFFLTGERDDMAGDFMALLLHQGFIEFRFDCGSGVGLVRSEETVLLNQWNHITLYRHRWDAWLQLNGGKHIQGRSKGLFSRITFREPLFIGGTGNTTGLSEKLPVSGGLKGCIRHLEVNDHLYKFALEPS---GEASKGFGIEECTADRCSRVPCQHGGKCLTSE----------------TS-------------AVCLCPLGFSGDLCEIRVDLEVPSFNGSSHLRYKGLGEEALTWLDLQVTLKPTAEDGLILYNGHRGNGIGDFMALYLRKGRVGFAYDLGTGVAEASSQHKINLGE 499          

HSP 2 Score: 127.872 bits (320), Expect = 3.254e-27
Identity = 104/377 (27.59%), Postives = 168/377 (44.56%), Query Frame = 0

Query:  214 CLCPHGKYGEKCALDRYISSPRFSGHSYLALPTL-TNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFG------LADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFD-NGDGVALVSNPEP--ECQGEQILALKPRSSGSLV 580
            CLCP G  G+ C +   +  P F+G S+L    L   A + L L V  +P +E+G++L  G   +  GD++AL +R G +    D GTG     ++ +++L  W+ + + R      L+++  +  E  S G F++++  Q + LGG  +F      + T S F GC++++EIN K     P     + L G + D CP   C + PC  +G CV       E D F                        C+C  G+  + C +      P F+G+    +F          S PL  D  V  +    DGL+L+    + GSGDFL+L L RG +   +    + + +V N     +    +I A++   SG LV
Sbjct:  397 CLCPLGFSGDLCEIRVDLEVPSFNGSSHLRYKGLGEEALTWLDLQVTLKPTAEDGLILYNGHRGNGIGDFMALYLRKGRVGFAYDLGTGVAEASSQHKINLGEWHEVRISRTGRLAILEVDNQIPIEVLSPGAFTQLSLPQNLYLGGVPNFNMVSPQIKTRSAFVGCVQKIEINGK-----PVSILAEALGGANVDNCPH-PCIAKPCGEDGECVP------EMDYF-----------------------TCRCKPGYRDQLCSR----GPPGFSGTDSYLHFNDPATLTTLVSDPL--DINVRFKITGSDGLILW---MDSGSGDFLSLGLERGALVLRYTVRAEEIVVVHNSSTVHDNLWHRIKAVRNGGSGLLV 729          

BLAST of PREDICTED: pikachurin vs. nr
Match: gi|1000768159|ref|XP_015607232.1| (PREDICTED: pikachurin-like [Cephus cinctus])

HSP 1 Score: 333.954 bits (855), Expect = 2.144e-100
Identity = 173/406 (42.61%), Postives = 242/406 (59.61%), Query Frame = 0

Query:  181 DKVYEIDAFCNLECGVEGECFMTR----DDKNV-------------IKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTG-HFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQGEQI 568
            + V   +  C+L+CG  G C++ R    D++++              ++RC CP G+ G++C L+  + SPRF+G  +LA P L  AY  +QL +EFRP + +GVLLL GE  D+ GD++ALI+  G IELR DCG+G GIVR+ + V L+ WN LTV+RH W  W+QLN+G   +GRS+GLFSRITF +P+ +GG G+   TG   L   SGF GC+R LE N   Y F P   QGD   G D +EC  D CS +PC + G C++  G                               VC CPLG++G+ CE  V++++P+FNGSS+L Y GLAD+S  W +  V L+  A DG+VLYNG+++D +GDF+AL+LS G V+F FD G G A + +  P   GE +
Sbjct:  175 ENVLSTEPACSLDCGSGGNCYIERYRSEDEEDLEGNGVTARQIQGSFRQRCQCPLGRSGDRCQLESEVRSPRFTGSGWLAFPALRAAYKHVQLELEFRPEAWDGVLLLAGERDDLQGDFMALILHHGFIELRFDCGSGVGIVRSSETVKLNEWNTLTVYRHRWDAWIQLNQGKRVQGRSKGLFSRITFREPLFVGGPGN--TTGLERLSVRSGFRGCVRHLEANEHRYRF-PLAPQGDAANGFDVEECTADRCSKVPCSHGGKCLTTGGD----------------------------TAVCLCPLGYTGDLCETRVDLQVPSFNGSSYLRYPGLADTSLSWLELAVTLKPTAPDGVVLYNGHHSDATGDFIALYLSAGHVQFTFDLGTGPATLRSETPVRLGEWL 549          

HSP 2 Score: 106.301 bits (264), Expect = 3.710e-20
Identity = 69/235 (29.36%), Postives = 112/235 (47.66%), Query Frame = 0

Query:  187 DAFCNLECGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTN-AYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPC 420
            D    + C   G+C  T  D  V    CLCP G  G+ C     +  P F+G SYL  P L + + S L+L+V  +P + +GV+L  G  +D TGD++AL +  GH++   D GTGP  +R+   V L  W  + V R      L++ +   +E  + G F++++    + LGG  +       + T + F GCI+ + +N +           + L G++   C    C + PC
Sbjct:  422 DRCSKVPCSHGGKCLTTGGDTAV----CLCPLGYTGDLCETRVDLQVPSFNGSSYLRYPGLADTSLSWLELAVTLKPTAPDGVVLYNGHHSDATGDFIALYLSAGHVQFTFDLGTGPATLRSETPVRLGEWLEVRVSRTGRLASLEVEDDPPQEILAPGAFTQLSLPLNLYLGGAPTPDMYSPKMKTTASFIGCIQTVILNGREVGI-----LAEALGGVNVGNCGH-ACEARPC 646          

BLAST of PREDICTED: pikachurin vs. nr
Match: gi|939627813|ref|XP_008546139.2| (PREDICTED: pikachurin [Microplitis demolitor] >gi|939627817|ref|XP_014295417.1| PREDICTED: pikachurin [Microplitis demolitor])

HSP 1 Score: 332.028 bits (850), Expect = 1.205e-99
Identity = 170/421 (40.38%), Postives = 241/421 (57.24%), Query Frame = 0

Query:  181 DKVYEIDAFCNLECGVEGECFM---------------------TRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTLTNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGTLTSQEQEYDDFNQPLLGVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSPLWNDFEVVLRSEAEDGLVLYNGNNNDGSGDFLALFLSRGFVEFAFDNGDGVALVSNPEPECQGEQILALKPRSSGSLV 580
            + V   +  C+L+CG  G C++                     TR  ++  K+RC CP G+ G++C  +  + SPRF+GH +LA P L  AY  +QL +EFRP + +G+L+LTGE  D+ GD++ALI+  G IE R DCG+G GIVR+ + V L+ WN L V+RH W  W+QLN G   +GRS+GLFSRITF +P+ +GG G+       L   +GF GCIR LE N   Y F P   QGD   G D +EC  D CS +PC + G C++  G                               VC CPLG++G+ CE  V++++P+FNGSS+L Y GL DSS  W +  ++L+   +DG++LYNG+++D +GDF+AL+LS G VEF FD G G A + +  P   GE  L ++   +G L 
Sbjct:  168 ENVVTTEPACSLDCGAAGNCYVEQFRNDEEGDGNGDDVARIDDTRPRRSSYKQRCQCPLGRTGDRCQNEAEVKSPRFTGHGWLAFPALKAAYKHIQLDLEFRPEAWDGILILTGERDDLQGDFMALILHHGFIEFRFDCGSGVGIVRSAETVKLNEWNTLNVYRHRWDAWIQLNGGKRVQGRSKGLFSRITFQEPLFVGGPGNTTGLDR-LPVRTGFKGCIRHLEANEHRYRF-PLAPQGDAANGFDVEECTADRCSKVPCSHGGKCLTTGGD----------------------------TAVCLCPLGYTGDLCETRVDLQVPSFNGSSYLRYPGLGDSSLSWLELSIILKPTTQDGVILYNGHHSDATGDFIALYLSSGHVEFTFDLGTGPATLRSESPVQLGEW-LEIRVSRTGRLA 557          

HSP 2 Score: 122.094 bits (305), Expect = 3.154e-25
Identity = 104/385 (27.01%), Postives = 175/385 (45.45%), Query Frame = 0

Query:  187 DAFCNLECGVEGECFMTRDDKNVIKKRCLCPHGKYGEKCALDRYISSPRFSGHSYLALPTL-TNAYSDLQLSVEFRPVSEEGVLLLTGESADMTGDYLALIIRDGHIELRLDCGTGPGIVRTRDQVHLHRWNRLTVFRHDWGVWLQLNEGVHEEGRSQGLFSRITFAQPVLLGGTGSFRNTGHFLDTNSGFHGCIRRLEINNKIYNFEPSERQGDTLFGMDTDECPEDTCSSIPCQNNGICVSAQGGGGEEDVFGGAFGT---LTSQEQEYDDFNQPLL-GVCQCPLGFSGEYCEQPVEVKIPAFNGSSHLTYFGLADSSP----LWNDFEVVLRSEAEDGLVLYNGNNN------DGSGDFLALFLSRGFVEFAFDNGDGVALV 556
            D    + C   G+C  T  D  V    CLCP G  G+ C     +  P F+G SYL  P L  ++ S L+LS+  +P +++GV+L  G  +D TGD++AL +  GH+E   D GTGP  +R+   V L  W  + V R      L++     +E  + G F++++    + LGG  S       + T + F GCI+ + +N++           + L G++   C    C + PC  +  C   +    E        GT     + E+      +P L       L    +Y     E  +P+F G+    ++  AD+         D  +  R+ + +GL+L++G  +      D + DFLAL L++G++  A++ G G A++
Sbjct:  419 DRCSKVPCSHGGKCLTTGGDTAV----CLCPLGYTGDLCETRVDLQVPSFNGSSYLRYPGLGDSSLSWLELSIILKPTTQDGVILYNGHHSDATGDFIALYLSSGHVEFTFDLGTGPATLRSESPVQLGEWLEIRVSRTGRLASLEVENEPAQEILAPGAFTQLSLPLNLYLGGAPSPEMFTPKMKTAASFVGCIQTVVLNHREVGI-----LAEALGGVNVGNCGH-ACEARPC-GDAECRPLR----ERFTCRCHPGTPHPCPAPEEFISSPPEPSLPASTSASLMLLSKY-----ERSVPSFTGTDSYLHYSDADTMKRIISYRVDINMRFRTSSSNGLLLWSGRQSDPQEQRDENEDFLALGLNQGYLTLAYNLGSGEAVL 783