K F94

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General

End Date: 

Monday, May 13, 2013 - 00:00

Experiment Batch ID: 

K

Start Date: 

Sunday, April 14, 2013 - 14:54

Experiment Contact: 

Sussie Dalvin
Sample

Developmental Stage CV: 

PreAdult2
Adult

Number of Individuals (Start): 

36

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

UN15_g1178

Primary target ID: 

EMLSAT00000009747, EMLSAT00000009747-705594 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Heidi Kongshaug

Fragment Label ID: 

F94

Fragment concentration: 

600.00ng/ul

Fragment length: 

445bp

Sense sequence: 

Aaaaggataaggaaccctcaccaccaagtaaaaaggcctccactacgagtctaaagaaaaaaacactagagcgacaaaaatccaagaaggaagagactgacaaagcagagaatgaggatggaaaaaactccataaaaagaaaggacacattccgacggcgattttcacttcgaatgaggaaaacagctttaaagcactcactttcatctgcaacaacttcaactgcaacaagcagcgaatatcaatccgatgatggaggagagtctgatgactattccgacgacgacgacgacgatgatgatgaggacactctcgtaaatagtatcaaactcagctcacagactttatcccttgggggagcagatgatcacttttctgataaggatggctcagcgcatcgactcacagaactcttcatcaacgacatcaatcttattgtggataaaactcttgctcttcctaatggcccagataaaattaagatgatccattctgttatagaatccattaaacccttgtacaacatggcgacactcaatcttca

Fragment Producer: 

licelab

Primer Group: 

b1473
Validation

Efficacy of Knock Down: 

F>70.00%
Phenotype

Phenotype CV: 

Number of Individuals (End): 

10

Gallery Image: 

Image file: 

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F94(UN15_g1178): Funny looking and short eggstrings in many individuals. Hatched copepodites looked normal

Sectioned animal RFK 13: ovaries very abnormal. Malformed eggs in GS, necrosis in some subcuticular tissue mailnly posterior in the cephalothorax. One type of gland like structures stain green, other glands appear normal.

 

 

The student Julia validated the RNAi lice with qPCR 130916

5' and 3' race have been performed