AV F404

Submitted by Andreas Borchel on 2 May, 2019 - 09:49

Phenotype CV:

General

Experiment Batch ID:

AV

Experiment Contact:

Andreas Borchel

Start Date:

14 March, 2019 - 08:35

End Date:

24 April, 2019 - 08:23

Experiment Description:

injection into pradult females

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult1

Adult

Target description

Primary target ID:

EMLSAT00000007125, EMLSAT00000007125-702972 (mRNA) Lepeophtheirus salmonis

EMLSAT00000010106, EMLSAT00000010106-705953 (mRNA) Lepeophtheirus salmonis

Fragment description

Fragment length:

400bp

Fragment concentration:

600ng/ul

Sense sequence:

TGTTGGCCCTATGAGTCCATCT TTTTTGTGACTTTGATGCCTTGA

Antisense sequence:

TGATCCTTGGAAGAAAAGAGGATG TTCATCATTTCCAGCATTGAGTTT

Fragment Label ID:

F404

Fragment Mixture Formula:

50% of both, in total 600 ng/ul

Fragment Producer:

licelab

Fragment Producer Contact:

Andreas Borchel

Validation

Efficacy of Knock Down:

F>50%

Phenotype

Number of Individuals (End):

15

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Knockdown of a testis-specific ion-pump in pre-adult1 males, checking reproduction in adults. Many males were lost, and no kncokdown observed.

AS F384

Submitted by Andreas Borchel on 21 November, 2018 - 11:10

Phenotype CV:

General

Experiment Batch ID:

AS

Experiment Contact:

Andreas Borchel

Start Date:

21 August, 2018 - 09:08

End Date:

24 September, 2018 - 09:08

Experiment Description:

Knockdown of one cement-gland gene.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000002328, EMLSAT00000002328-698175 (mRNA) Lepeophtheirus salmonis

EMLSAT00000011195, EMLSAT00000011195-707042 (mRNA) Lepeophtheirus salmonis

EMLSAT00000010070, EMLSAT00000010070-705917 (mRNA) Lepeophtheirus salmonis

Fragment description

Fragment concentration:

600ng/ul

Fragment Batch ID:

F384

Fragment Label ID:

F384

Fragment Mixture Formula:

F381+F382+F383 in same ratio

Validation

Efficacy of Knock Down:

F>99%

Phenotype

Number of Individuals (End):

21

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knockdown lead to missing egg strings

RNAi_AS 11369+12062

Submitted by Anna Komisarczuk on 21 November, 2018 - 10:09

Phenotype CV:

General

Experiment Batch ID:

RNAi_AS

Experiment Contact:

Anna Komisarczuk

Start Date:

21 August, 2017 - 13:24

End Date:

24 September, 2018 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Developmental Stage CV:

preadult II femal

Target description

Primary target ID:

EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis

EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis

Gene or target symbol:

Nervana

Fragment description

Fragment Label ID:

F389

Validation

Efficacy of Knock Down:

F>90%

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developmenta details, no egg-strings, no gut contant. All genes downregulated by around 90%

RNAi_AS 11366+11369+10832

Submitted by Anna Komisarczuk on 21 November, 2018 - 10:07

Phenotype CV:

General

Experiment Batch ID:

RNAi_AS

Experiment Contact:

Anna Komisarczuk

Start Date:

21 August, 2017 - 13:24

End Date:

24 September, 2018 - 00:00

Experiment Description:

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Developmental Stage CV:

preadult II femal

Target description

Primary target ID:

EMLSAG00000011366, EMLSAG00000011366-694132 (gene) Lepeophtheirus salmonis

EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis

EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis

Gene or target symbol:

Nervana

Target type:

External DBref:

Fragment description

Sense sequence:

Antisense sequence:

Fragment Batch ID:

Fragment Label ID:

F388

Fragment Mixture Formula:

Fragment Producer:

Validation

Efficacy of Knock Down:

F>90%

Phenotype

Phenotype:

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developmenta details, no egg-strings, no gut contant. All genes downregulated by around 90%

AS F380

Submitted by Andreas Borchel on 21 November, 2018 - 09:59

Phenotype CV:

General

Experiment Batch ID:

AS

Experiment Contact:

Andreas Borchel

Start Date:

21 August, 2018 - 09:08

End Date:

24 September, 2018 - 09:08

Experiment Description:

Knockdown of one cement-gland gene.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000000454, EMLSAT00000000454-696301 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAT00000000454

Fragment description

Fragment length:

500bp

Fragment concentration:

600ng/ul

Sense sequence:

GTCCACTCGTATGCCCACCTCTCTGTGAGGAACATGAAATGTTTTGTCCCGGATCCATGGTTGATGAATGTCCTACAGAAGATAATTGCGTTGCCAAAATTGATGAAAATAACAAGTGTCCAAATTATTGTCCAATTACTTGTGAAAAAGGTCAACACATATGTCCTGGAATCCGAAAAGATGATGGATGCAGAGGTCCAGATACCTGTGTACCCATTGGTACACAATGTCCAAGTGAGATTTTTGATGAATTTGCTTGTCCCATCATTGATTCACCCAGTTGTGTGTATCCCGAAACTCATTGCCATGGTGGTTACAATTCAAGGGGTTGTCGAAAACGATCCTTCTGTAAGGAATTAAAGAATAAAACAGAGCATCAACATGGTGTAAATGGCATTGATGGTTGTCCAACATTTTGTCCAGCAACATGCATTCCAGGTATTGAGACAGAATGCCCTGAAACCTACGATGAAGATGGATGTCCTCTTCCTGCGTCTTGC

Fragment Batch ID:

F380

Fragment Label ID:

F380

Validation

Efficacy of Knock Down:

F>99%

Phenotype

Number of Individuals (End):

20

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knockdown lead to missing egg strings

RNAi_AS 11366+12062

Submitted by Anna Komisarczuk on 21 November, 2018 - 09:58

Phenotype CV:

General

Experiment Batch ID:

RNAi_AS

Experiment Contact:

Anna Komisarczuk

Start Date:

21 August, 2017 - 13:24

End Date:

24 September, 2018 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Developmental Stage CV:

preadult II femal

Target description

Primary target ID:

EMLSAG00000011366, EMLSAG00000011366-694132 (gene) Lepeophtheirus salmonis

EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis

Gene or target symbol:

Nervana

Fragment description

Fragment Label ID:

F386

Validation

Efficacy of Knock Down:

F>90%

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delevlopmental defects, empty intestine, no egg strings. Both genes are down-regulated by around 90%

AS F379

Submitted by Andreas Borchel on 21 November, 2018 - 09:46

Phenotype CV:

General

Experiment Batch ID:

AS

Experiment Contact:

Andreas Borchel

Start Date:

21 August, 2018 - 09:08

End Date:

24 September, 2018 - 09:08

Experiment Description:

Knockdown of one cement-gland gene.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000008518, EMLSAT00000008518-704365 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAT00000008518

Fragment description

Fragment length:

483bp

Fragment concentration:

600ng/ul

Sense sequence:

GGACGGTGTGTGACTCCAAACACCTGCAAGTGTGATTCTGGGTGGAGGGGTGATACCTGCAATGAGTGTATTCCCTCATCTCAGTGTCAAAATGGATTTTGCGTTCATTCTCCGAAGGAATGTATCTGCAGTTTGGGCTGGGCTGGAGTAAACTGTGATCTACAGGATCATGGATGTGTCCATGGAACCTTTTCATCAAAACATCACTGCACATGCGATCCGGGATGGTTAGGAAGAAATTGCAGTTCTTGTATACCGCACCCAAGTTGTGTTTGGGGATCATGTAAATCCACTCCGGGCGAATGTTTTTGTGATCCAGGATGGGATGGAAGGAATTGTGACATCCCCATTTGCGAGAAAGGGTGTCATCCAGATCACGGATTTTGTTTTAAGCCATCTCAATGTATATGTCGCTCTGGTTGGCATGGAAAACGTTGTGATCTTTGCACTCCAATGCCTGGATGCACACGAGGATGCCATAAA

Fragment Batch ID:

F379

Fragment Label ID:

F379

Validation

Efficacy of Knock Down:

F>90%

Phenotype

Number of Individuals (End):

15

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knockdown lead to missing egg strings

RNAi_AN 10832+11366+11369+12062

Submitted by Anna Komisarczuk on 14 June, 2018 - 13:31

Phenotype CV:

General

Experiment Batch ID:

RNAi_AN

Experiment Contact:

Anna Komisarczuk

Start Date:

1 November, 2017 - 13:24

End Date:

3 January, 2018 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

both

Developmental Stage CV:

preadult I male

preadult I female

Target description

Primary target ID:

EMLSAG00000010832, EMLSAG00000010832-693598 (gene) Lepeophtheirus salmonis

EMLSAG00000011366, EMLSAG00000011366-694132 (gene) Lepeophtheirus salmonis

EMLSAG00000011369, EMLSAG00000011369-694135 (gene) Lepeophtheirus salmonis

EMLSAG00000012062, EMLSAG00000012062-694828 (gene) Lepeophtheirus salmonis

Fragment description

Fragment Label ID:

F345

Validation

Efficacy of Knock Down:

F>90%

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delevlopmental defects, empty intestine, no egg strings

AL F339

Submitted by Joakim Brunet on 14 June, 2018 - 13:14

Phenotype CV:

General

Experiment Batch ID:

RNA_AL

Experiment Contact:

Joakim Brunet

Start Date:

28 August, 2017 - 10:00

End Date:

6 October, 2017 - 00:00

Experiment Description:

Knockdown of the nuclear receptor FTZF1

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000008902, EMLSAG00000008902-691668 (gene) Lepeophtheirus salmonis

EMLSAG00000008900, EMLSAG00000008900-691666 (gene) Lepeophtheirus salmonis

Gene or target symbol:

FTZ-F1

Target type:

mRNA

Fragment description

Fragment length:

499bp

Sense sequence:

gtgatagggcacggaaactgcaagtcttgcgt Caaagacaagtgatacacggtggagtttccatgggcccaagtagcatgaataatacgaca Accacaacgagtagtagtgcatcctacggcggcggtgcctccggtggaatgaactccacg Agtagccctccatccatgaacaataataataataacaattccccctcaatctacggctca Cccatcactcatattaaacaagagatacaaataccacaagtcacatctatgacgtcttca Ccagatacttcaccctctcccgttcatttacccggaatgatttctaatcctggaggcaat Ccaatgggtggagatactccgggctccaatcccatcattgcccaacagactgatcctgcc Ggaggtattcaatggcagatcaatgctgctggaaagcatcaggatatccaaagtaatcaa Ggaactgggaaaatgccagccatcattcgagaattccttgcctccct

Fragment ID:

FTZF1 DsRNA probe 2, FTZF1 DsRNA 2 (RNAi_reagent) Lepeophtheirus salmonis

Fragment Batch ID:

F339

Fragment Label ID:

F339

Fragment Producer:

licelab

Fragment Producer Contact:

Joakim Brunet

Validation

Efficacy of Knock Down:

F>45%

Phenotype

Number of Individuals (End):

7

Phenotype:

No eggstrings

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Knockdown of FTZF1 targetting both isoforms, alpha and beta. Only alpha is downregulated, few survivors. Sectioning reveals disorganization of eggs in genital segment, resulting in no eggstrings, same as probe F338 (other entry).

AL F338

Submitted by Joakim Brunet on 14 June, 2018 - 12:56

Phenotype CV:

General

Experiment Batch ID:

RNA_AL

Experiment Contact:

Joakim Brunet

Start Date:

28 August, 2017 - 10:00

End Date:

6 October, 2017 - 10:00

Experiment Description:

Knockdown of the nuclear receptor Fushi tarazu factor 1 (FTZF1)

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000008902, EMLSAG00000008902-691668 (gene) Lepeophtheirus salmonis

EMLSAG00000008900, EMLSAG00000008900-691666 (gene) Lepeophtheirus salmonis

Gene or target symbol:

FTZ-F1

Target type:

mRNA

Fragment description

Fragment length:

467bp

Sense sequence:

cgccttcatcatcgacaacaacatccatactaacctcatctacaaataaaaaacctcgttccctcaaaatcgtcgaatgtctcgactct tcctcgacgacacacaccacagcctctccatcgtcgccatcaacctcttcatggaatctc Attcttccttcgcatccttccgtagaatcaccttcatcgtcttcgagtccttctcatcaa Ggagggggagataaagagatatcctctgtggttatagcagcctctacgaccacgacagca Actaaaagatcatttagtcaacatatcatggaagaagaggaagaggacgaagacgaagaa Gaggctggaggacacttatcttggaaccctgatgaagagcaggatcaatcagctgaggag Gatgcaggactaccaacagcatccagtaacaacaactccgctggatccacatccggagacttcggtcctggagttgtg

Fragment ID:

FTZF1 DsRNA probe 1, FTZF1 DsRNA 1 (RNAi_reagent) Lepeophtheirus salmonis

Fragment Batch ID:

F338

Fragment Label ID:

F338

Fragment Producer:

licelab

Fragment Producer Contact:

Joakim Brunet

Validation

Efficacy of Knock Down:

F>45%

Phenotype

Number of Individuals (End):

12

Phenotype:

No eggstrings

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RFAL12.jpg

RFAL11_s123_1_genitalsegment_25x.jpg

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Knockdown of the nuclear FTZ-F1 in pre-adult 2. Knockdown resulted in strange genital segments. Sectioning revealed disorganization of eggs in genital segment.