female reproduction

RNAi_AS 11369+12062

Phenotype CV: 

General

Experiment Batch ID: 

RNAi_AS

Experiment Contact: 

Start Date: 

21 August, 2017 - 13:24

End Date: 

24 September, 2018 (All day)
Sample

Organism: 

Sex: 

female

Developmental Stage CV: 

preadult II femal
Target description
Fragment description

Fragment Label ID: 

F389
Validation

Efficacy of Knock Down: 

F>90%
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developmenta details, no egg-strings, no gut contant. All genes downregulated by around 90%

RNAi_AS 11366+11369+10832

Phenotype CV: 

General

Experiment Batch ID: 

RNAi_AS

Experiment Contact: 

Start Date: 

21 August, 2017 - 13:24

End Date: 

24 September, 2018 (All day)

Experiment Description: 

Sample

Organism: 

Sex: 

female

Developmental Stage CV: 

preadult II femal
Target description
Fragment description

Sense sequence: 

Antisense sequence: 

Fragment Batch ID: 

Fragment Label ID: 

F388

Fragment Mixture Formula: 

Fragment Producer: 

Validation

Efficacy of Knock Down: 

F>90%
Phenotype

Phenotype: 

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developmenta details, no egg-strings, no gut contant. All genes downregulated by around 90%

RNAi_AS 11366+12062

Phenotype CV: 

General

Experiment Batch ID: 

RNAi_AS

Experiment Contact: 

Start Date: 

21 August, 2017 - 13:24

End Date: 

24 September, 2018 (All day)
Sample

Organism: 

Sex: 

female

Developmental Stage CV: 

preadult II femal
Target description
Fragment description

Fragment Label ID: 

F386
Validation

Efficacy of Knock Down: 

F>90%
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delevlopmental defects, empty intestine, no egg strings. Both genes are down-regulated by around 90%

RNAi_AN 10832+11366+11369+12062

Phenotype CV: 

General

Experiment Batch ID: 

RNAi_AN

Experiment Contact: 

Start Date: 

1 November, 2017 - 13:24

End Date: 

3 January, 2018 (All day)
Sample

Organism: 

Sex: 

both

Developmental Stage CV: 

preadult I male
preadult I female
Target description
Fragment description

Fragment Label ID: 

F345
Validation

Efficacy of Knock Down: 

F>90%
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delevlopmental defects, empty intestine, no egg strings

AL F339

Phenotype CV: 

General

Experiment Batch ID: 

RNA_AL

Experiment Contact: 

Start Date: 

28 August, 2017 - 10:00

End Date: 

6 October, 2017 (All day)

Experiment Description: 

Knockdown of the nuclear receptor FTZF1
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description
Fragment description

Fragment length: 

499bp

Sense sequence: 

gtgatagggcacggaaactgcaagtcttgcgt Caaagacaagtgatacacggtggagtttccatgggcccaagtagcatgaataatacgaca Accacaacgagtagtagtgcatcctacggcggcggtgcctccggtggaatgaactccacg Agtagccctccatccatgaacaataataataataacaattccccctcaatctacggctca Cccatcactcatattaaacaagagatacaaataccacaagtcacatctatgacgtcttca Ccagatacttcaccctctcccgttcatttacccggaatgatttctaatcctggaggcaat Ccaatgggtggagatactccgggctccaatcccatcattgcccaacagactgatcctgcc Ggaggtattcaatggcagatcaatgctgctggaaagcatcaggatatccaaagtaatcaa Ggaactgggaaaatgccagccatcattcgagaattccttgcctccct

Fragment ID: 

Fragment Batch ID: 

F339

Fragment Label ID: 

F339

Fragment Producer: 

licelab

Fragment Producer Contact: 

Validation

Efficacy of Knock Down: 

F>45%
Phenotype

Number of Individuals (End): 

7

Phenotype: 

No eggstrings
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Knockdown of FTZF1 targetting both isoforms, alpha and beta. Only alpha is downregulated, few survivors. Sectioning reveals disorganization of eggs in genital segment, resulting in no eggstrings, same as probe F338 (other entry).

AL F338

Phenotype CV: 

General

Experiment Batch ID: 

RNA_AL

Experiment Contact: 

Start Date: 

28 August, 2017 - 10:00

End Date: 

6 October, 2017 - 10:00

Experiment Description: 

Knockdown of the nuclear receptor Fushi tarazu factor 1 (FTZF1)
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description
Fragment description

Fragment length: 

467bp

Sense sequence: 

cgccttcatcatcgacaacaacatccatactaacctcatctacaaataaaaaacctcgttccctcaaaatcgtcgaatgtctcgactct tcctcgacgacacacaccacagcctctccatcgtcgccatcaacctcttcatggaatctc Attcttccttcgcatccttccgtagaatcaccttcatcgtcttcgagtccttctcatcaa Ggagggggagataaagagatatcctctgtggttatagcagcctctacgaccacgacagca Actaaaagatcatttagtcaacatatcatggaagaagaggaagaggacgaagacgaagaa Gaggctggaggacacttatcttggaaccctgatgaagagcaggatcaatcagctgaggag Gatgcaggactaccaacagcatccagtaacaacaactccgctggatccacatccggagacttcggtcctggagttgtg

Fragment ID: 

Fragment Batch ID: 

F338

Fragment Label ID: 

F338

Fragment Producer: 

licelab

Fragment Producer Contact: 

Validation

Efficacy of Knock Down: 

F>45%
Phenotype

Number of Individuals (End): 

12

Phenotype: 

No eggstrings
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Knockdown of the nuclear FTZ-F1 in pre-adult 2. Knockdown resulted in strange genital segments. Sectioning revealed disorganization of eggs in genital segment.

AL Hormone receptor 3 (HR3)

Phenotype CV: 

General

Experiment Batch ID: 

RNA_AL

Experiment Contact: 

Start Date: 

28 August, 2017 - 10:00

End Date: 

6 October, 2017 - 10:00

Experiment Description: 

Knockdown of the ortholog of the nuclear receptor HR3. Injection of pre-adult 2 females
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

Gene or target symbol: 

EMLSAG00000000733

Target type: 

mRNA
Fragment description

Fragment length: 

441bp

Sense sequence: 

gccta Gcaccattcgagcaagaaatctcattgcaaaacaaacaaacaaaaaggattcatagaata Aatagaaaaaggaccctacaaggcaaaaaaaacagcaactattttttacaaattgtttcg Tactcgtcgatgcaaaatcagaaatattatacgaatttgtcctcaatggattggatatca Ggctatcgtcttccatccaattcggatttaaagtgtgatgatgatcaccatggcggcggg Gcaggagtacagcatgcatctgttgtcggcggaccaacaggcggagcactaggatctggc Tcttccggtcccctcacaaacaacaccaattccaactcttcctcaccctccatgctaggc Atgtgggcagcagctgtggccaatacacctcttaaagtggaaacagctgatcatcatgga Aatggatctctggtgg

Fragment Batch ID: 

F341

Fragment Label ID: 

F341

Fragment Producer: 

licelab

Fragment Producer Contact: 

Validation

Efficacy of Knock Down: 

F>90%
Phenotype

Number of Individuals (End): 

17

Phenotype: 

No eggstrings. Disorganization of eggs in genital segment
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Knockdown of HR3 in pre-adult 2 females. Resulted in no eggstring production. Sections reveals disorganized eggs in genital segment. Ovaries look normal.

AQ F375 EMLSAG00000008959

Phenotype CV: 

General

Experiment Batch ID: 

RAQ

Experiment Contact: 

Start Date: 

16 April, 2018 - 09:00

End Date: 

23 May, 2018 - 09:00

Experiment Description: 

Knock-down of EMLSAG00000008959
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

Gene or target symbol: 

EMLSAG00000008959
Fragment description

Fragment length: 

432bp

Fragment concentration: 

600ng/ul

Sense sequence: 

gcctccggttcggatgaagaagtagaagaggaagaaataactgaatatgatgccttgttaaaggtgtatggagctcaaacatatgttgatgaagctgaagaggaagagatatgcgaaggagaaatggaggaggaagaaaacgatatggatgaggaagatgtatcattgaatggagtagatgggatagatgaagaagatttattgtcgaatgaagaagaaaatgaaactatcgatctcgaaagtggagaagatgaaaatgaaactatcgatctcgaaagtggagaagaggaaaatgaaactatcgatctcgaaagtggagaagaagaagaagagattgtggatactgatatcggaagkgrtatattagatgaaaatgaggatgaatttccagagatggatgacgaaaawgacacttgtggcccctctgatcct

Fragment Label ID: 

F375

Fragment Producer: 

licelab

Fragment Producer Contact: 

Phenotype

Number of Individuals (End): 

5

Phenotype: 

no eggstreng
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Knock-down of EMLSAT00000008959

AP F366 EMLSAT00000001458

Phenotype CV: 

General

Experiment Batch ID: 

AP

Experiment Contact: 

Start Date: 

6 March, 2018 - 09:00

End Date: 

10 April, 2018 - 09:00

Experiment Description: 

EMLSAG1458 knock-down
Sample

Organism: 

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

Gene or target symbol: 

EMLSAG00000001458
Fragment description

Fragment length: 

412bp

Fragment concentration: 

600ng/ul

Sense sequence: 

>F366 caagctgttattgattggcgattctggtgtaggcaaatcgtgcttactattaagatttgctgatgatacttacacagaaagttacataagtacaattggtgttgactttaagattagaacaattgaactcgacggaaagactatcaagttgcaaatctgggatactgcaggccaagaaagattcagaactatcacatccagttactatcgtggtgcccacggaatcatcgttgtatatgacgttacggaccaggattcttttaataaccttaagcaatggcttcaagaaattgatagatacgcatgtgaaaatgtaaacaagcttcttgtgggaaacaaatgtgatctgacaacaaagaaggttgtagattatacaactgcaaaagaatacgctgatcagttaaatatgccg

Fragment Label ID: 

F366

Primer Group: 

likb2502_T7_b2515

Fragment Producer: 

licelab

Fragment Producer Contact: 

Validation

Efficacy of Knock Down: 

F>77%
Phenotype

Number of Individuals (End): 

13

Phenotype: 

no eggstreng
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Knock-down of EMLSAT00000001458.

Mucin-like spermatophore wall protein 2 (AJ F270)

Phenotype CV: 

General

Experiment Batch ID: 

AJ

Experiment Contact: 

Start Date: 

21 March, 2017 - 09:08

End Date: 

25 April, 2017 - 09:08

Experiment Description: 

injection into young adut males, checking reproduction & spermatophore attachment of females
Sample

Organism: 

Sex: 

male

Number of Individuals (Start): 

30

Developmental Stage CV: 

Adult
Target description

Primary target ID: 

Gene or target symbol: 

MLSWP2
Fragment description

Fragment length: 

497bp

Fragment concentration: 

600ng/ul

Sense sequence: 

CCAGGCTTTTCACACGCTTT

Antisense sequence: 

GCTCTTGGCCGAGCATAAAA

Fragment Batch ID: 

F270

Fragment Label ID: 

F270
Validation

Efficacy of Knock Down: 

F>80%
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male specific gene; checking spermatophore attachment to female

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