F18/F84

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General

End Date: 

Wednesday, July 16, 2014 - 00:00

Experiment Batch ID: 

F17/F84

Start Date: 

Friday, July 11, 2014 - 13:11

Experiment Contact: 

Liv Sandlund

Publication: 

The Ecdysone Receptor (EcR) is a Major Regulator of Tissue Development and Growth in the Marine Salmonid Ectoparasite; Lepeophtheirus salmonis (Copepoda; Caligidae)
Sample

Developmental Stage CV: 

Nauplius

Number of Individuals (Start): 

200

Sex: 

unknown

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

EcR
USP

Primary target ID: 

EMLSAT00000005090, EMLSAT00000005090-700937 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Label ID: 

F18
F84

Fragment concentration: 

15.00ng/ul

Fragment length: 

667bp

Fragment Mixture Formula: 

1500ug was used of each fragment
Phenotype

Phenotype: 

No molting

Number of Individuals (End): 

150

Gallery Image: 

Image file: 

Image icon 140711_F18_F84_45x.JPG
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Nauplia were bathed in both EcR and USP fragment. A distinct phenotype where the animals did not molt into copepodids were visible. The animals seem to have certain characteristics of the copepodid, meaning some developmental processes has begun. I suspect the animals would have died after a few more days. Knockdown was significant for fragments for EcR with 52 % but nor for USP 30 %.