F18/F84

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General

End Date: 

Wednesday, November 26, 2014 - 00:00

Experiment Batch ID: 

F18/F84

Start Date: 

Wednesday, November 19, 2014 - 13:33

Experiment Contact: 

Liv Sandlund

Publication: 

The Ecdysone Receptor (EcR) is a Major Regulator of Tissue Development and Growth in the Marine Salmonid Ectoparasite; Lepeophtheirus salmonis (Copepoda; Caligidae)
Sample

Developmental Stage CV: 

Nauplius

Number of Individuals (Start): 

200

Sex: 

unknown

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

EcR
UsP

Target type: 

Nuclear receptor
Nuclear receptor

Primary target ID: 

EMLSAT00000005090, EMLSAT00000005090-700937 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Label ID: 

F18
F84

Fragment concentration: 

15.00ng/ul

Fragment length: 

667bp
Validation

Efficacy of Knock Down: 

F>50.00%
Phenotype

Phenotype: 

No molting

Number of Individuals (End): 

150
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Experiment with knockdown of EcR and USP together in nauplia was repeated. Same phenotype was seen with no molting from nauloia II to copoepodite but some copepodite features were seen. Knockdown of EcR was 47 % Knockdown of USP 67 % Both significant.