C F22

Submitted by on

General

End Date: 

Monday, September 24, 2012 - 00:00

Experiment Batch ID: 

c

Start Date: 

Tuesday, August 14, 2012 - 12:54

Experiment Contact: 

Frank Nilsen
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

39

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

g1751_Tryp1

Primary target ID: 

EMLSAT00000003030, EMLSAT00000003030-698877 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Heidi Kongshaug

Fragment Label ID: 

F22

Fragment concentration: 

600.00ng/ul

Sense sequence: 

tggctattattgcatctgctttggctaatcctaaatatccagatatttgtggattggaaaacaaccctttagtggattcgaaaatcgttggaggacaggaagcagcaaggcatcagtttccatggctagcttctatatatacctttggttcttgtactggttctattttggatgaaagttggatcatcactgctaaacattgtgtgaaaactgatggtgttgcctcagttcttgttggggttcatagcattgcaaatggggcagatgaaccatatagacaaaaccgaaagagttccgaaattcacgtatcatcaagtggagactttgccttaatcaaattggagagccccttggaattcaatgaatatgtcagacccgtttgcataccaacacgtgcagatgctaacgagacctttgttggagaatcaacgaccataacaggatggggtgtaaaagttatgcaatgtggttggccagaaaatattatctgtactgatgcaaacccaggaaaatctatttgttttggtgacagcggtggccctcttaactacgaaatg

Fragment Producer: 

licelab

Primer Group: 

g1751_tryp1F_b828
Validation

Efficacy of Knock Down: 

F>94.00%
Phenotype

Number of Individuals (End): 

5
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EMLSAT00000003030 g1751_Tryp1 er en blanding av 3030 og 3029 Tryp 1(rack8): 5 females, appeared normal. qPCR: Tryp1: Significant downregulation (both with primers inside and outside the dsRNA fragments, both tested)