D F28

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General

End Date: 

Wednesday, October 3, 2012 - 00:00

Experiment Batch ID: 

d

Start Date: 

Friday, August 24, 2012 - 13:22

Experiment Contact: 

Frank Nilsen
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

40

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

g158_glycin_trans

Primary target ID: 

EMLSAT00000006302, EMLSAT00000006302-702149 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Heidi Kongshaug

Fragment Label ID: 

F

Fragment concentration: 

600.00ng/ul

Fragment length: 

525bp

Sense sequence: 

ttgaggacgttgggtttgctctcgaatctaaattaaattatagtcttaaacgatttattaaatgtacttttatatcaatttgtggactcatttgtactctagtcttttccacaagtttgggattggcactttatcataccataattgagtacttttttgggataactgaagtttttattgcattattgacgatagtttcaatctcttacatttatggacttcgactttggagtaatgctcttcactacatgcttaaaacagaatgcagaatattttattctagctggcttgtcatttgtccactagttattttgtacgttttgattgtaaggctctctgatttgaaattggataactctatggagctcgtcatcgctgctgttcaatctcttttaatccttcagattcctatatgcgcattctgcaaattcttctactctggaatcaattgctcgagggaactttttgtaccttcatctgattggggtcttcgatctaaaagaattcccacaggctcaatgcatg

Primer Group: 

g158_glycinF_b834
Validation

Efficacy of Knock Down: 

F>42.00%
Phenotype

Number of Individuals (End): 

12
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GlycinT, many short eggstrings (15102), 2 almost empty strings (ødipus), bad appetite in some individuals qPCR:Glycin: Significantly downregulated (but only with primers outside the dsRNA fragments, both tested)