E F49a

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General

End Date: 

Monday, November 26, 2012 - 00:00

Experiment Batch ID: 

e

Start Date: 

Monday, October 15, 2012 - 14:52

Experiment Contact: 

Sussie Dalvin
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

35

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

ILR frag2

Primary target ID: 

EMLSAT00000002641, EMLSAT00000002641-698488 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Heidi Kongshaug

Fragment Label ID: 

F49

Fragment concentration: 

600.00ng/ul

Fragment length: 

417bp

Sense sequence: 

atccttcggagtctgaatgtgacgatcatgttaccacaacaccattgctcccacagagtacttgcgaagattcttgtaccgatcacaaatgctcagaagaatttgagacttggggtaatcctgaaaacatagctacctactatgaatgttcatttaattgcgcttgcttaataaactgtccacttggattggtttatgatagtaatgcagcctattgtaaagaacctgattgctcaacccaaccagagagtccagcatgcagcgcaattcctatcaattccaaagttgtgaggaacgttcagttttttaatgaacctctatgtgagcataatggaaacacgacatgtacctttgagaattcaccctcaactacatgcccatggcatttcttcgtttgttttggagaatggacttggt

Fragment Producer: 

licelab

Primer Group: 

ILR_f4_b1226
Validation

Efficacy of Knock Down: 

F>0.10%
Phenotype

Number of Individuals (End): 

22
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ILR (F49): 22 (76%) and 25 (64%) females recovered, all appear normal. Normal cops. F49 No significant downregulation NB! 40% upregulation