basement membrane-specific heparan sulfate proteoglycan core, maker-scaffold781_size98004-snap-gene-0.18 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. L. salmonis genes
Match: EMLSAG00000001502 (supercontig:LSalAtl2s:LSalAtl2s1258:70737:78110:1 gene:EMLSAG00000001502 transcript:EMLSAT00000001502 description:"maker-LSalAtl2s1258-snap-gene-0.30") HSP 1 Score: 198.364 bits (503), Expect = 7.377e-55 Identity = 102/227 (44.93%), Postives = 132/227 (58.15%), Query Frame = 0
Query: 85 CDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPS 311
C+ +F CN+G+CI + L CDGTP C DSTDEI C + CD + +W+C + CIDKR+ CDGYPDC D SDED + CP+ +C EEWQF+C D++RCID RR CDGY+DCLD SDE DC +V C YWQFKC + G C+D+RR+CDG NDC DE +C ++ + I E P T+ P+ + DC PS
Sbjct: 80 CNSTIEFACNDGKCIDKALLCDGTPHCHDSTDEISCKS-------------------------SNNCDGDDEWKCLSGDVCIDKRKRCDGYPDCHDLSDEDIDQCPERDCNEEWQFKCWDRKRCIDSRRLCDGYTDCLDGSDEVDCLSVVCQYWQFKCLLDGXCVDSRRKCDGYNDCNDGSDETNCKGLSTQTY-----IFEHSPKTI-PVKLFSNVILDCNVVNPS 275 HSP 2 Score: 142.51 bits (358), Expect = 5.864e-36 Identity = 128/402 (31.84%), Postives = 179/402 (44.53%), Query Frame = 0
Query: 42 IPEAQCSRT-DFICEDLACINFALRCDGKFDCEDRSDEYQCPPS--CDPNTQFTCNNG-RCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQF----KCRISGLCIDTRRRCDGTNDCGSDEDE-QDCDNIT-------------ENPFFSTNEIEEPV---------PTTVSPISIEVLPEDDCPTFCP--SMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLD--GSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPN 408
I AQC+ T +F C D CI+ AL CDG C D +DE C S CD + ++ C +G CI + RCDG P C D +DE D C +C+EE Q++C + +RCID RRLCDGY DC D SDE C V C + WQF+C C+D RR+CDGY+DC D SDE +C + + F K L + C+ N G E Q+ + T N +E V VS + + VL C C ++ VCG++GL Y + C L SC+ +I+ C + + CA+++ +C++ G CI CD DC D DEK+C + + + N
Sbjct: 75 IFSAQCNSTIEFACNDGKCIDKALLCDGTPHCHDSTDEISCKSSNNCDGDDEWKCLSGDVCIDKRKRCDGYPDCHDLSDE------------------------DIDQCPERDCNEEWQFKCWDRKRCIDSRRLCDGYTDCLDGSDEVD--CLSVVC-QYWQFKCLLDGXCVDSRRKCDGYNDCNDGSDETNCKGLSTQTYIFEHSPKTIPVKLFSNVILDCNVVNPSGKWTYEGQNVHDSTSAGLEGRLGTAFKNGSLLIRNFQKEDVGYYTCASSGGNEVSSVYV-VLDASSCKKECKDDEFAQRVCGSNGLIYSSLCDLTKASCNLKIKIRPVAMSKC----NRLTTSKCAIDERECVNPSGKCIKETSFCDGIPDCEDKGDEKFCFRKDMKSVIIGKN 444
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. L. salmonis genes
Match: EMLSAG00000002033 (supercontig:LSalAtl2s:LSalAtl2s136:1281404:1312976:1 gene:EMLSAG00000002033 transcript:EMLSAT00000002033 description:"maker-LSalAtl2s136-augustus-gene-13.17") HSP 1 Score: 132.88 bits (333), Expect = 1.429e-32 Identity = 97/236 (41.10%), Postives = 127/236 (53.81%), Query Frame = 0
Query: 51 DFICEDLACINFALRCDGKFDCEDRSDEYQ--CPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF---------DPCVPGE-CDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAV---ECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDE 267
DF C++ CI CDG DC D SDE Q P+C N F C NGRCI + CDG C D +DE GC ++ FS NGR I +T+ D G+ C E+T Q+ CA+ CI + +CDG DC D +DE E CP + C QF+C ++++CI + +CDG DC D SDE C ++ +C QFKC+ SG+CI R CDGT+DCG DE
Sbjct: 2136 DFTCDNQRCIPKTWVCDGDDDCLDNSDENQNCTAPTCSEN-DFQCGNGRCIPKGYFCDGDKDCDDFSDERGCQNVT-CESNQFSCTNGRCIPMTWKCDGENDCGDSSDEGDFCAEKTCAYFQFSCASSGHCIPQSWVCDGDNDCFDNADE--EGCPPITCSAS-QFKCNNQKQCIHESYKCDGIPDCEDASDELGCPSIAPDQCADQQFKCKTSGICIPIRWHCDGTSDCGDGSDE 2366 HSP 2 Score: 100.908 bits (250), Expect = 2.858e-22 Identity = 79/228 (34.65%), Postives = 108/228 (47.37%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDY----WQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
CS ++F C + CI +CD + DC D SDE C P F C +G CI +C+G C D +DE+ C +P F +S C EE ++EC NH C+++ CDG DC D +DED E C C E +F+C + +CI + CDG C D SDEN+ E + F C +G CI + CD +DCG DE+ C
Sbjct: 4887 CSESEFKCGNGKCIQSRWKCDNENDCGDNSDEADCGTWVCPADTFQCKSGHCIKLSDQCNGEKDCLDFSDELDC------SPRFNNSY----------------CPEE-KFECKNH-LCVNQNDRCDGKQDCGDGTDEDPEECKSFVCDEAHRFKCIN-NKCIPRYNVCDGIDQCGDGSDENNITRCESTRRFCPFMFTCS-NGNCISKSKICDLHDDCGDLSDERGC 5088 HSP 3 Score: 99.7525 bits (247), Expect = 7.108e-22 Identity = 132/435 (30.34%), Postives = 182/435 (41.84%), Query Frame = 0
Query: 43 PEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNN-GRCIGQDLRCDGTPQCQDSTDEIG-CGAISKVAPGFFSSLNGRIIGLTFD---PCVPGECDEETQWECAN--------------HRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN----DCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTF------CPSMSRPVCGTDGLTYENE-CRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCA---VNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
PE C F C+D C+ CDG DC + DE C C P+ QF C + G+CI +CDG C D +DE + F N R I L + G+ +E + C N + RCI + CDG DC D SDE E C V C E+ F+C+++ RCI K CD +DC D+SDE D +C +FKC +G CI +R +CD NDCG + DE DC + + + +S + E DC F P + C + +N C + C Q+ +G T+EDP E + ++++C++ CI CD C DG DE I + P FTC +G+C CD DC D SDE C
Sbjct: 4676 PERFCGENKFQCKDNQCLLLTTLCDGHNDCTEGEDEELCDSEC-PDNQFKCKSTGKCIIDSWKCDGDNDCPDGSDENKEMCENQECKDDEFQCNNKRCIPLLWKCDFDDDCGDDSDEVAFLCRNRNCTAGWKKCPGHANYRCIPEFLYCDGKDDCRDGSDELDENC--VPCDEKTHFKCKNR-RCIPKTWLCDFENDCGDKSDEKEKICDGQYRDCSESEFKCG-NGKCIQSRWKCDNENDCGDNSDEADC-----GTWVCPADTFQCKSGHCIKLSDQCNGEKDCLDFSDELDCSPRFNNSYCPEEKFECKNHLCVNQNDRCDGKQDCG---DG---TDEDPEECKSFVCDEAHRFKCINNKCIPRYNVCDGIDQCGDGSDE-----NNITRCESTRRFCPF-MFTCSNGNCISKSKICDLHDDCGDLSDERGC 5088 HSP 4 Score: 89.3521 bits (220), Expect = 1.151e-18 Identity = 117/393 (29.77%), Postives = 153/393 (38.93%), Query Frame = 0
Query: 83 PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEND-CFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNIT--ENPFFSTNE---IEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQ---EIQIYYEGPCETEEDPVEPQGCAV-----NQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGS---------CRPIDTRCDGMVDCEDESDEFDCPLGSTSKP 452
P+C + FTC+N RCI + CDG C D++DE C C E ++C N R CI K CDG DC D SDE C V C E QF C + RCI +CDG +DC D SDE D C C Y+QF C SG CI CDG NDC + DE+ C IT + F N+ I E P + E CP+ P ++C + C + I+ + +G + + EP C N ++C + C+Y + + D S+E + P EF C GS C PID CD D FDCP G+ P
Sbjct: 2129 PACKHSWDFTCDNQRCIPKTWVCDGDDDCLDNSDE-------------------------NQNCTAPTC-SENDFQCGNGR-CIPKGYFCDGDKDCDDFSDERG--CQNVTC-ESNQFSCTNG-RCIPMTWKCDGENDCGDSSDEGDFCAEKTCAYFQFSCASSGHCIPQSWVCDGDNDCFDNADEEGCPPITCSASQFKCNNQKQCIHESYKCDGIPDCEDASDELGCPSIAP---------------DQCADQQFKCKTSGICIPIRWHCDGTSDCGDGSDEPTTCGKIDCQENYFKCKNSKCVY--KSFVCDGEDDCDGDGSDESLEHACKAP---------EFVCPSGSWKCPGISDHCVPIDKICD---------DHFDCPNGADEGP 2455 HSP 5 Score: 85.1149 bits (209), Expect = 2.649e-17 Identity = 103/303 (33.99%), Postives = 127/303 (41.91%), Query Frame = 0
Query: 144 DPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPC--ETEEDPVEPQGCAVNQWQCLD-GSCIYLKQQCDRKYDCRDGEDEKYC-SIETIDEIPAPPNCDPLDEFTCK-DGSCRPIDTRCDGMVDCEDESDE 441
+P VP C + C N +RCI K +CDG DC D SDE+ + C C E FQC + RCI K CDG DC D SDE C V C+ QF C +G CI +CDG NDCG DE D E C F S C + G C ++ C D + C +E+ P C+ +Q++C + CI+ +CD DC D DE C SI AP C +F CK G C PI CDG DC D SDE
Sbjct: 2125 EPPVPA-CKHSWDFTCDN-QRCIPKTWVCDGDDDCLDNSDEN-QNCTAPTCSEN-DFQCGNG-RCIPKGYFCDGDKDCDDFSDERGCQNVTCESNQFSC-TNGRCIPMTWKCDGENDCGDSSDEGD-----------------------------FCAEKTCAYFQFS-----CASSG-----HCIPQSWVCDGDND--------CFDNADEEGCPPITCSASQFKCNNQKQCIHESYKCDGIPDCEDASDELGCPSI-------APDQCAD-QQFKCKTSGICIPIRWHCDGTSDCGDGSDE 2366 HSP 6 Score: 85.1149 bits (209), Expect = 2.913e-17 Identity = 70/215 (32.56%), Postives = 95/215 (44.19%), Query Frame = 0
Query: 59 CINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKC-----RISGLCIDTRRRCDGTNDCGSDEDEQ 268
CI +CD DC+ DEY C C NG C+ + +CDG C+D +DE C S CD E ++ CA+ +CI + + C+ +C D SDE P G QF+C++ CI R RCDGY DC D +DE +C A+ C +F C +S CID + CDG DC DE+
Sbjct: 1214 CIPERWKCDYHRDCDGGEDEYDCQYRVCQKDDHHCGNGFCVPNEKKCDGYHNCRDKSDEEDCPGTS--------------------------CDLE-EFRCASGEKCIAQYQKCNHREECEDGSDEKDCNFPPCHSG---QFRCQN-HLCIPARWRCDGYRDCTDGTDEFNCTAIACPENKFNCPKGDSDLSPKCIDKSKLCDGHADCDDGADER 1397 HSP 7 Score: 78.9518 bits (193), Expect = 1.965e-15 Identity = 62/185 (33.51%), Postives = 84/185 (45.41%), Query Frame = 0
Query: 43 PEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDE-YQCP---PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRR--RC---DGY 218
P+ +CS T+F C+ CIN +CD DC+D SDE +C +C+P T+FTC N +CI Q+ RCD C D++DE C S P E ++C + CI ++CD DC D +DE C K EC QC C+D + RC DGY
Sbjct: 4110 PKRECSDTEFRCDTGKCINLKWKCDHANDCDDGSDEPKECKDHYRTCNPETEFTCQNSKCINQNYRCDDEDDCGDNSDEFHCDKASIACP-------------------------EGHFKCQRSKECIPYEKVCDKVSDCTDNTDEPLH-CNKNECDMIEDNQC--GHYCVDTKESYRCECKDGY 4266 HSP 8 Score: 78.5666 bits (192), Expect = 2.774e-15 Identity = 86/267 (32.21%), Postives = 109/267 (40.82%), Query Frame = 0
Query: 55 EDLACINFALRCDGKFDCEDRSDEYQCPPSCDPN----TQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQ-------------------------------------------RCIDKRRRCDGYSDCLDESDENDCFAVECD-YWQFKCRISGLCIDTRRRCDGTNDCG--SDEDEQDCD 271
+D CI +CDGK DCED SDE PPSC +F C + +C+ CDG C + DE C + EC + Q++C + +CI CDG DCPD SDE+ EMC EC ++ +FQC +K+ RCI + CDG DC D SDE D V CD FKC+ + CI CD NDCG SDE E+ CD
Sbjct: 4648 KDDRCIPHYEKCDGKTDCEDGSDE---PPSCPERFCGENKFQCKDNQCLLLTTLCDGHNDCTEGEDEELCDS---------------------------EC-PDNQFKCKSTGKCIIDSWKCDGDNDCPDGSDENKEMCENQECKDD-EFQCNNKRCIPLLWKCDFDDDCGDDSDEVAFLCRNRNCTAGWKKCPGHANYRCIPEFLYCDGKDDCRDGSDELDENCVPCDEKTHFKCK-NRRCIPKTWLCDFENDCGDKSDEKEKICD 4881 HSP 9 Score: 77.0258 bits (188), Expect = 9.579e-15 Identity = 126/438 (28.77%), Postives = 165/438 (37.67%), Query Frame = 0
Query: 53 ICEDLACINFALRCDG-KFDC---------------------EDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGC----------GAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE--NDCFAVE--CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPT-FCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDE-KYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKP 452
+ E+ C+N C+G KF C D S+ Y +C P ++F C NGRCI + RCD C D +DE C I K+ + N D C+ + Q C + C+ LCDG DC D SDE C C F+C D QRCI CDG DC D SDE + C A + C F C +G CI CDG NDC + DE PE +C C + C + + C K C + + + E + C+ +++C G CI LK +CD DC DG DE K C C+P EFTC++ C + RCD DC D SDEF C S + P
Sbjct: 3811 VNENKMCVNSTYNCEGDKFTCANGKCVSRLWACDGDDDCGDNSDESENYCSYHTCKP-SEFRCGNGRCIFETWRCDHEDDCGDRSDEEECDYPKCAEGNKQMIVKILQYLMKTSN-----YDKDKKKDISCNVD-QLICNSTTICVQHYWLCDGDDDCGDNSDESPVHCAARTCPPN-TFRCPD-QRCIPATWYCDGEKDCEDGSDEPEDACNAEDRTCFGDLFTCD-NGNCIPKIYVCDGDNDCNDNSDES--------------------------------PERECSKRTCDTEEEFECKKNIAWNRSGCIPKRYVCDGEADCVDGADESLEHANCTRPKRECSDTEFRCDTGKCINLKWKCDHANDCDDGSDEPKECKDHY-------RTCNPETEFTCQNSKCINQNYRCDDEDDCGDNSDEFHCDKASIACP 4199 HSP 10 Score: 70.0922 bits (170), Expect = 1.370e-12 Identity = 73/218 (33.49%), Postives = 100/218 (45.87%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP-PSCDPNTQFTCNNG-RCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDE---DAEMCP--KVECGE---EWQFQCRDKQRCIDKRRRCDGYSDCLDESDEND-CFAVECD--YWQFKCRIS---GLC 248
C + D C + C+ +CDG +C D+SDE CP SCD +F C +G +CI Q +C+ +C+D +DE C F PC G Q+ C NH CI R CDGY DC D +DE A CP K C + + +C DK + CDG++DC D +DE C CD + +F C+ S G+C
Sbjct: 1241 CQKDDHHCGNGFCVPNEKKCDGYHNCRDKSDEEDCPGTSCDLE-EFRCASGEKCIAQYQKCNHREECEDGSDEKDC---------------------NFPPCHSG------QFRCQNHL-CIPARWRCDGYRDCTDGTDEFNCTAIACPENKFNCPKGDSDLSPKCIDKSKL------CDGHADCDDGADERTACSKSLCDSLFCEFDCKSSLEGGIC 1423 HSP 11 Score: 60.4622 bits (145), Expect = 1.012e-9 Identity = 68/233 (29.18%), Postives = 99/233 (42.49%), Query Frame = 0
Query: 40 DDIPEAQCSRT------DFICED------LACINFALRCDGKFDCEDRSDEYQCPPSCD------PNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECG-EEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE-NDCFAVECDYWQFKCRISGLCIDTR 252
D+ PE +CS+ +F C+ CI CDG+ DC D +DE +C +T+F C+ G+CI +CD C D +DE K + + C+ ET++ C N +CI++ CD DC D SDE C K E F+C+ + CI + CD SDC D +DE C ECD + + C+DT+
Sbjct: 4050 DESPERECSKRTCDTEEEFECKKNIAWNRSGCIPKRYVCDGEADCVDGADESLEHANCTRPKRECSDTEFRCDTGKCINLKWKCDHANDCDDGSDE------PKECKDHYRT-----------------CNPETEFTCQN-SKCINQNYRCDDEDDCGDNSDE--FHCDKASIACPEGHFKCQRSKECIPYEKVCDKVSDCTDNTDEPLHCNKNECDMIEDN-QCGHYCVDTK 4255 HSP 12 Score: 59.6918 bits (143), Expect = 1.872e-9 Identity = 40/121 (33.06%), Postives = 58/121 (47.93%), Query Frame = 0
Query: 156 QWECANHRRCIDKRRLCDGYPDCPDQSDE---DAEMCPK--VECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCD 271
Q+EC CI +R CD + DC DE +C K CG + C+ ++CDGY +C D+SDE DC CD +F+C CI ++C+ +C DE+DC+
Sbjct: 1205 QFECGTSGNCIPERWKCDYHRDCDGGEDEYDCQYRVCQKDDHHCGNGF---------CVPNEKKCDGYHNCRDKSDEEDCPGTSCDLEEFRCASGEKCIAQYQKCNHREECEDGSDEKDCN 1316
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. L. salmonis genes
Match: EMLSAG00000007936 (supercontig:LSalAtl2s:LSalAtl2s469:287745:294486:-1 gene:EMLSAG00000007936 transcript:EMLSAT00000007936 description:"augustus_masked-LSalAtl2s469-processed-gene-3.13") HSP 1 Score: 127.102 bits (318), Expect = 8.895e-31 Identity = 135/454 (29.74%), Postives = 190/454 (41.85%), Query Frame = 0
Query: 31 NSSEGLMPQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDEE---------TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAV--------------ECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYY---------EGPCETEEDPVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEKYCSI-ETIDEIPAPPNCDPLDEFTCKDG-SCRPIDTRCDGMVDCEDESDEFDC 444
N + G + D+ + C F C + CI RCD K DC D SDE C N QF C NG+CI RCD C D+TDE GC ++ + A G F N + I + + C G +E+ +++C++ +C CD + DC D DE + C + C +EWQF+C D Q CI K CDG DC D SDE +C + C +FKC +G CI +CDG NDC DE +C F+ + I + TF P + L C S ++I + +G E E C ++ +C G CI L + C+ + DC D DEK C I +P+ CDP+ F+C + C C+G+ +CED SDE +C
Sbjct: 1047 NPASGSICMDNSTHSTCGEGMFRCVNKLCIPLNWRCDDKNDCGDGSDELNCRNVTCTNYQFKCGNGKCIPHGWRCDDEDDCYDNTDEQGCDSL-ECAEGHFKCKNDQCIKSEYRCDFDEDCRDGSDEEDCKMREKCMINEFKCSSSSKCFPISWQCDNFADCEDGLDE--KNCEEKTC-KEWQFKCND-QTCIYKDWVCDGQLDCADNSDEFNCSSTSTSMEHQIPSFQHNNCTEDKFKCA-NGKCIPINWKCDGHNDCPDQSDEMNCG-------FNHHYIH-----------------NGSTTFYPEPKKKYI-----------ELNKFECKSGKKIWQAWICDGYNDCPDGSDENHSLCQEKMRCKPHESRCEFSGECINLDKICNGRKDCVDESDEKGCGHDHNIFPMPSSILCDPI-HFSCDNNQKCLSHSFICNGIRECEDGSDEDNC 1458 HSP 2 Score: 92.0485 bits (227), Expect = 1.712e-19 Identity = 100/316 (31.65%), Postives = 142/316 (44.94%), Query Frame = 0
Query: 178 CPDQSDEDAEMC----PKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNI--TENPFFSTNE--IEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQE-IQIYYE----GPCE--TEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETID---EIPA--PPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPL-------GSTSKPSEPEPTTADPFEYE 466
CPD++ +C CGE F+C +K CI RCD +DC D SDE +C V C +QFKC +G CI RCD +DC + DEQ CD++ E F N+ I+ + E+DC +C + CSS + I ++ CE +E E + C Q++C D +CIY CD + DC D DE CS + +IP+ NC D+F C +G C PI+ +CDG DC D+SDE +C GST+ EP+ + ++E
Sbjct: 1043 CPDENPASGSICMDNSTHSTCGEG-MFRCVNK-LCIPLNWRCDDKNDCGDGSDELNCRNVTCTNYQFKCG-NGKCIPHGWRCDDEDDCYDNTDEQGCDSLECAEGHFKCKNDQCIKSEYRCDFDEDCRDGSDEEDCKM-----------------REKCMINEFKCSSSSKCFPISWQCDNFADCEDGLDEKNCEEKTCKEWQFKCNDQTCIYKDWVCDGQLDCADNSDEFNCSSTSTSMEHQIPSFQHNNCTE-DKFKCANGKCIPINWKCDGHNDCPDQSDEMNCGFNHHYIHNGSTTFYPEPKKKYIELNKFE 1337
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. L. salmonis genes
Match: EMLSAG00000011270 (supercontig:LSalAtl2s:LSalAtl2s772:2760:23028:-1 gene:EMLSAG00000011270 transcript:EMLSAT00000011270 description:"maker-LSalAtl2s772-snap-gene-0.12") HSP 1 Score: 108.612 bits (270), Expect = 1.107e-24 Identity = 132/437 (30.21%), Postives = 178/437 (40.73%), Query Frame = 0
Query: 49 RTDFI-CEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCN-NGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDE-----------DAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCD-NITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVN-----QWQCL---DGSCIYLKQQCDRKYDCRDGEDE------KYCSIETIDEIPAPP-----NCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKP 452
++ FI C + CI + +C+G DC D SDE C C N F C+ G C+ + +CDG P C+D++DEI C ++ A S +I+ CA CI CDG DC D SDE +++ CP FQC RCI + CD DC DESDE +C C QFKC +G CI + CD T DC DE DC + F N +P P+++ T CP P+ G + N+ R C D Y C D EP+ C + + C+ D CI ++ CD +C D DE KY S+ P +CD +F CK+ +C D+ CDG DC D SDE C + P
Sbjct: 2591 KSGFIHCTNGRCIPESSKCNGIDDCGDFSDETGC--FCPENKPFVCSIGGPCLEEHRKCDGVPDCEDTSDEINCPKVNCAASNISSFDESKIL----------------LENCAYTTACILPEWRCDGKNDCWDNSDEKNCDNSSIVGSESDHCPL------GTFQCLGDNRCISESWVCDRDKDCKDESDEQNCHYY-CKEHQFKCN-NGQCIPKKWYCDRTPDCSDQSDEMDCKVECHSDSEFRCNSTGVCIPKLWVCDGNSDCPKNEDETGCPIYLAPL----GNCHPNQFRCFNGICV-DNSYYCDYNDDCLDGSD--EPETCDYSFRPKRLYCCVRSGDMHCISPQKLCDGISNCDDESDELQSTCSKYSSVIISSNQSQSPVFVLGSCDDQSKFECKNKACVSWDSLCDGQNDCGDYSDEASCDINECENP 2994 HSP 2 Score: 105.531 bits (262), Expect = 8.487e-24 Identity = 128/436 (29.36%), Postives = 162/436 (37.16%), Query Frame = 0
Query: 44 EAQCSRTDFICEDLACINFALRCDGKFDCEDRSDE--YQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEE----------WQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC--------FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNE---IEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDP------VEPQG-CAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCD----PLDEFTC---KDGSCRPIDTRCDGMVDCEDESDEF 442
E QCS D CI + L CDG C D SDE C + C NGRCI + +C+G C D +DE GC C E + C+ C+++ R CDG PDC D SDE CPKV C C CI RCDG +DC D SDE +C + C F+C CI CD DC + DEQ+C + F N I + +P + E DC C S S C + G+ C K C + + C ED + P G C NQ++C +G C+ CD DC DG DE P CD P + C D C CDG+ +C+DESDE
Sbjct: 2552 EFQCSAND------VCILYTLTCDGLNHCPDGSDEDLKYCAIRHCKSGFIHCTNGRCIPESSKCNGIDDCGDFSDETGCF-----------------------------CPENKPFVCSIGGPCLEEHRKCDGVPDCEDTSDEIN--CPKVNCAASNISSFDESKILLENCAYTTACILPEWRCDGKNDCWDNSDEKNCDNSSIVGSESDHCPLGTFQCLGDNRCISESWVCDRDKDCKDESDEQNCHYYCKEHQFKCNNGQCIPKKWYCDRTPDCSDQSDEMDCKVECHSDSEFRCNSTGV-----CIPKLWVCDGNSD--------CPKNEDETGCPIYLAPLGNCHPNQFRCFNGICVDNSYYCDYNDDCLDGSDE-------------PETCDYSFRPKRLYCCVRSGDMHCISPQKLCDGISNCDDESDEL 2924 HSP 3 Score: 99.7525 bits (247), Expect = 6.727e-22 Identity = 98/274 (35.77%), Postives = 127/274 (46.35%), Query Frame = 0
Query: 44 EAQCSRTDFICED-LACINFALRCDGKFDCEDRSDE-------------YQC-----------PPSCDPNT----QFTCNNGRCIGQDLRCDGTPQCQ----DSTDEIGCGAISKVAPGFFSS----LNGRIIGLTFDPC--VPGECDEETQWECANHR------RCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDC--GSDEDEQDC 270
++ CS+TDF+CE+ L CI F +CDG+ DC D DE +QC P +C T F C NGRCI + +CDG P C+ +S+DE I+ F +N R D C E D C+ H+ CI++ LCDG DC D+SDE C +V C E +F+C CI K RCDG DC+D SDEN C C F C S CI ++ CDG C GSDED++ C
Sbjct: 3392 KSNCSKTDFVCENTLKCIPFWWKCDGQDDCGDGLDEPLDCPPFKCHAGEFQCNNGNCVLPIHEPINCPNRTCSEELFKCPNGRCIPKVWKCDGDPDCELGEDESSDECSVDLITHCEGSFICKNKHCINERWRCDQQDDCGDNSDEIDCMDFKNCSLHQVPCSNGNCIEESELCDGNFDCQDRSDE--LYCSQV-CNPEEEFRCASPPTCIPKEWRCDGDRDCVDGSDENRCPQSHCRVGHFSCG-SSECISSQWLCDGEAXCVDGSDEDDEAC 3661 HSP 4 Score: 97.4413 bits (241), Expect = 3.736e-21 Identity = 115/401 (28.68%), Postives = 154/401 (38.40%), Query Frame = 0
Query: 58 ACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDE---IGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDC--LDESDENDC--------FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITEN-PFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEK---YCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDE 441
AC + D K CE + E PP QFTC+NG CI +L+CDG C+D +DE I C I+ CD++ Q+ CAN CI+ +CD Y DC D SDED +C CG++ FQC++ C+ C+ DC D SDE +C + C +F C CI CD DC + DE +C N E F N + + S L DD G+D EN C L + C ++ + CA + G CI CD C DG DE +C + +CD + C +G C CDG +DC D SDE
Sbjct: 885 ACRDGYFLKDDKVSCE--AIEGWVPPPYCAKDQFTCSNGHCIDLNLKCDGEIDCEDGSDENLLIACQNIT--------------------------CDKDNQFMCANKASCIELESICDRYDDCMDGSDEDPGLCLSRTCGDD-MFQCKETGICLHYFSMCNSVYDCGGNDISDEGNCNNGSSKNELPLFCLPNEFSCDADSSCIPISGVCDDEKDCSDNSDELNCHNENETCKGFLCNRDSKCI--NASKKCDGALDCDD-------------GSD----ENYCSLSSTKCGKEEFM-------------------CARS------GDCIDKANVCDGVVHCSDGSDEHNNVFCGLS---------DCDLSTQRKCANGECILKGYWCDGHLDCTDNSDE 1203 HSP 5 Score: 96.2857 bits (238), Expect = 8.416e-21 Identity = 80/251 (31.87%), Postives = 112/251 (44.62%), Query Frame = 0
Query: 52 FICEDLACINFALRCDGKFDCEDRSDEYQCP--PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCID--------------------------KRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC---FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCD 271
FIC++ CIN RCD + DC D SDE C +C + Q C+NG CI + CDG CQD +DE+ C + F + + C+P E W C R C+D + LCDG C D SDED E C + C E +++C ++ +C+ CDG DC DESDE+ + C C C+ CDG +DCG+++DE+DC+
Sbjct: 3511 FICKNKHCINERWRCDQQDDCGDNSDEIDCMDFKNCSLH-QVPCSNGNCIEESELCDGNFDCQDRSDELYCSQVCNPEEEFRCA--------SPPTCIPKE------WRCDGDRDCVDGSDENRCPQSHCRVGHFSCGSSECISSQWLCDGEAXCVDGSDEDDEACSRHLC-EPNRYRCDNRHQCVLWTSVCDGIPDCSDESDESQVACSLSSSCPPESVSCGEVNRCVSKEALCDGFDDCGNEQDERDCE 3745 HSP 6 Score: 93.2041 bits (230), Expect = 6.529e-20 Identity = 69/201 (34.33%), Postives = 91/201 (45.27%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNG-RCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECD----------EETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC 230
CS C + CI + CDG FDC+DRSDE C C+P +F C + CI ++ RCDG C D +DE C S G FS + I + CV G + E ++ C N +C+ +CDG PDC D+SDE C C + RC+ K CDG+ DC +E DE DC
Sbjct: 3545 NCSLHQVPCSNGNCIEESELCDGNFDCQDRSDELYCSQVCNPEEEFRCASPPTCIPKEWRCDGDRDCVDGSDENRCPQ-SHCRVGHFSCGSSECISSQWLCDGEAXCVDGSDEDDEACSRHLCEPNRYRCDNRHQCVLWTSVCDGIPDCSDESDESQVACSLSSSCPPESVSCGEVNRCVSKEALCDGFDDCGNEQDERDC 3744 HSP 7 Score: 92.8189 bits (229), Expect = 1.045e-19 Identity = 92/317 (29.02%), Postives = 129/317 (40.69%), Query Frame = 0
Query: 151 CDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNIT-ENPFFSTNEIEEPVPTTVSPISIEVLPE------------------DDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVE-PQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKD-GSCRPIDTRCDGMVDCEDESDEFDCPL 446
C + +++C+ + CI CDG CPD SDED + C C + C + RCI + +C+G DC D SDE CF E F C I G C++ R+CDG DC DE +C + S+ + + + + + +LPE D+ GT +N C ++ C D++ C+ E D C +Q++C +G CI K CDR DC D DE C +E C EF C G C P CDG DC DE CP+
Sbjct: 2547 CQQTNEFQCSANDVCILYTLTCDGLNHCPDGSDEDLKYCAIRHCKSGF-IHCTNG-RCIPESSKCNGIDDCGDFSDETGCFCPENK--PFVCSIGGPCLEEHRKCDGVPDCEDTSDEINCPKVNCAASNISSFDESKILLENCAYTTACILPEWRCDGKNDCWDNSDEKNCDNSSIVGSESDHCPLGTFQCLGDNRCISESWVCDRDKD--------CKDESDEQNCHYYCKEHQFKCNNGQCIPKKWYCDRTPDCSDQSDEMDCKVE----------CHSDSEFRCNSTGVCIPKLWVCDGNSDCPKNEDETGCPI 2841 HSP 8 Score: 88.1965 bits (217), Expect = 2.786e-18 Identity = 80/264 (30.30%), Postives = 110/264 (41.67%), Query Frame = 0
Query: 42 IPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPP----SCDPNTQFTC-NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISK-------------VAPGFFSSLNGRIIGLTFDPCVPGECDEET------------QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQ----FQCRDKQRCIDKRRRCDGYSDCLDESDENDC--FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQD 269
+P C++ F C + CI+ L+CDG+ DCED SDE +CD + QF C N CI + CD C D +DE +S+ + +FS N D G C+ + ++ C CI +CD DC D SDE + C E + F C +CI+ ++CDG DC D SDEN C + +C +F C SG CID CDG C DE +
Sbjct: 906 VPPPYCAKDQFTCSNGHCIDLNLKCDGEIDCEDGSDENLLIACQNITCDKDNQFMCANKASCIELESICDRYDDCMDGSDEDPGLCLSRTCGDDMFQCKETGICLHYFSMCNSVYDCGGNDISDEGNCNNGSSKNELPLFCLPNEFSCDADSSCIPISGVCDDEKDCSDNSDE-------LNCHNENETCKGFLCNRDSKCINASKKCDGALDCDDGSDENYCSLSSTKCGKEEFMCARSGDCIDKANVCDGVVHCSDGSDEHN 1162 HSP 9 Score: 80.1073 bits (196), Expect = 8.380e-16 Identity = 121/390 (31.03%), Postives = 162/390 (41.54%), Query Frame = 0
Query: 77 DEYQCPPSCDPNTQFTCNNG-RCIGQDLRCDGTPQCQDSTDE-IGCGAISKVAPGFFSSLNGRIIGLTFDP--CVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGE-EWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE-CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTD-GLTYENECRLKAMSCSSDQEIQIYY----EGPC----ETEEDPVEPQGCAVNQWQCLD-GSCIYLKQQCDRKYDCRDGEDEK--YCSIETIDEIPAPPNCDPLDEFTCKD-GSCRPIDTRCDGMVDCEDESDEFDCPLG 447
D C +C T F C N +CI +CDG C D DE + C K G F NG + +P C C EE ++C N R CI K CDG PDC DE ++ C E F C++K CI++R RCD DC D SDE DC + C Q C +G CI+ CDG DC DE C + NP E P T P + DC V G+D ++ CR+ SC S + I + E C + +++ C N+++C + C+ CD DC D DE CS+ + PP + +C + C + CDG DC +E DE DC G
Sbjct: 3387 DGLTCKSNC-SKTDFVCENTLKCIPFWWKCDGQDDCGDGLDEPLDCPPF-KCHAGEFQCNNGNCVLPIHEPINCPNRTCSEEL-FKCPNGR-CIPKVWKCDGDPDCELGEDESSDECSVDLITHCEGSFICKNKH-CINERWRCDQQDDCGDNSDEIDCMDFKNCSLHQVPCS-NGNCIEESELCDGNFDCQDRSDELYCSQVC-NP---EEEFRCASPPTCIPKEWRCDGDRDC----------VDGSDENRCPQSHCRVGHFSCGSSECISSQWLCDGEAXCVDGSDEDDEACSRHLCEPNRYRCDNRHQCVLWTSVCDGIPDCSDESDESQVACSLSS----SCPP-----ESVSCGEVNRCVSKEALCDGFDDCGNEQDERDCEKG 3747 HSP 10 Score: 78.1814 bits (191), Expect = 3.356e-15 Identity = 69/229 (30.13%), Postives = 98/229 (42.79%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNN-GRCIGQDLRCDGTPQCQDSTDEIGC----GAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDEET--------QWEC---ANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKV------------------ECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECD 236
C F C + CI CD DC D+SDE C C +++F CN+ G CI + CDG C + DE GC + P F NG + ++ D C+ G + ET + C + CI ++LCDG +C D+SDE C K C ++ +F+C++K C+ CDG +DC D SDE C EC+
Sbjct: 2765 CKEHQFKCNNGQCIPKKWYCDRTPDCSDQSDEMDCKVECHSDSEFRCNSTGVCIPKLWVCDGNSDCPKNEDETGCPIYLAPLGNCHPNQFRCFNGICVDNSYYCDYNDDCLDGSDEPETCDYSFRPKRLYCCVRSGDMHCISPQKLCDGISNCDDESDELQSTCSKYSSVIISSNQSQSPVFVLGSCDDQSKFECKNKA-CVSWDSLCDGQNDCGDYSDEASCDINECE 2992 HSP 11 Score: 63.5438 bits (153), Expect = 1.424e-10 Identity = 45/124 (36.29%), Postives = 60/124 (48.39%), Query Frame = 0
Query: 155 TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECG--EEWQFQCRDKQRCIDKRRRCDGYSDC------LDESDENDCF---AVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDE 267
Q+EC + ++CID + CDG PDC D+SDE + E G +E F C+ CI R CD DC LD+SDE+ V C Q C+ + C+ R CD DC + DE
Sbjct: 49 NQYECTDGQKCIDFSKKCDGVPDCLDESDEGEDCLKSQESGNCKEGHFLCKKSGECIPLRWICDNELDCGNSTLGLDKSDESSSLCRKNVTCAANQLMCKDNTTCMHLSRFCDHKIDCPDNSDE 172 HSP 12 Score: 59.6918 bits (143), Expect = 1.882e-9 Identity = 37/89 (41.57%), Postives = 50/89 (56.18%), Query Frame = 0
Query: 180 DQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN-DCFAVE----CDYWQFKCRISGLCIDTRRRCDGTNDCGS 263
+++D + E K+ C Q++C D Q+CID ++CDG DCLDESDE DC + C F C+ SG CI R CD DCG+
Sbjct: 32 EKTDHENERLKKLYCLSN-QYECTDGQKCIDFSKKCDGVPDCLDESDEGEDCLKSQESGNCKEGHFLCKKSGECIPLRWICDNELDCGN 119 HSP 13 Score: 56.9954 bits (136), Expect = 1.231e-8 Identity = 49/149 (32.89%), Postives = 67/149 (44.97%), Query Frame = 0
Query: 43 PEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCD----PNTQFTC---NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDE 184
P C F C + C++ + CD DC D SDE P +CD P + C + CI CDG C D +DE+ SK + SS + V G CD+++++EC N + C+ LCDG DC D SDE
Sbjct: 2845 PLGNCHPNQFRCFNGICVDNSYYCDYNDDCLDGSDE---PETCDYSFRPKRLYCCVRSGDMHCISPQKLCDGISNCDDESDELQ-STCSKYSSVIISSNQSQSPVF-----VLGSCDDQSKFECKN-KACVSWDSLCDGQNDCGDYSDE 2983 HSP 14 Score: 52.7582 bits (125), Expect = 2.889e-7 Identity = 47/145 (32.41%), Postives = 60/145 (41.38%), Query Frame = 0
Query: 43 PEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDE--YQCPPS-CDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDE 184
P++ C F C CI+ CDG+ C D SDE C C+PN N +C+ CDG P C D +DE S+VA SS C P C RC+ K LCDG+ DC ++ DE
Sbjct: 3621 PQSHCRVGHFSCGSSECISSQWLCDGEAXCVDGSDEDDEACSRHLCEPNRYRCDNRHQCVLWTSVCDGIPDCSDESDE------SQVACSLSSS------------CPPESV------SCGEVNRCVSKEALCDGFDDCGNEQDE 3741
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. L. salmonis genes
Match: EMLSAG00000009473 (supercontig:LSalAtl2s:LSalAtl2s60:645074:747260:1 gene:EMLSAG00000009473 transcript:EMLSAT00000009473 description:"maker-LSalAtl2s60-snap-gene-6.10") HSP 1 Score: 96.2857 bits (238), Expect = 2.118e-21 Identity = 85/240 (35.42%), Postives = 122/240 (50.83%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP-PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC-FA-----VECDY-WQFKCRISG-LCIDTRRRCDGTNDCG--SDEDEQDCDNITE 275
C +F C++ CI CD + DC D SDE CP SC+ + +F+C +G CI + RCDG C D++DE C IS + G FS N + +C ++T+++C + CI + LCD DCPD SDE + C QF+C + CI +C G +C+D SDE +C FA + CD +F C G +C+ + CD N+CG +DE + C NI E
Sbjct: 111 CISNEFTCDNSKCIQNRWVCDQEDDCGDGSDEKSCPDMSCNADNEFSCGDGYCIKKYWRCDGDVDCPDASDERDCPEIS--SSGNFSDEN-----------IIVKC-KDTEFQCLSRNHCIHRTWLCDKDTDCPDGSDESVQQCGTRNKCRTDQFKCVNGD-CIPGHLQCSGAPECIDNSDEENCNFANGESSLGCDADKEFDCVGDGRMCVPLEQVCDKMNNCGNWADESHEKC-NINE 334 HSP 2 Score: 94.7449 bits (234), Expect = 5.825e-21 Identity = 104/359 (28.97%), Postives = 146/359 (40.67%), Query Frame = 0
Query: 89 TQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGE---EWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDY-WQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTC-KDGS-CRPIDTRCDGMVDCEDESDE 441
T+F C+N +CI Q RC+G C D++DE+ C S F CDE RC+ K CDG DC D SDE+ ++C C +F C D +CI R CD DC D SDE C + C+ +F C G CI RCDG DC DE+DC I+ + FS +E + + L N C + C D + +G E+ + C +Q++C++G CI QC +C D DE+ C+ + + CD EF C DG C P++ CD M +C + +DE
Sbjct: 29 TKFECDNKQCIPQSWRCNGLDDCMDNSDEVNCIVKSNCTDSEF------------------RCDE---------IRCVPKNWQCDGGEDCIDGSDENEDICSNQTCDRTCISNEFTC-DNSKCIQNRWVCDQEDDCGDGSDEKSCPDMSCNADNEFSCG-DGYCIKKYWRCDGDVDCPDASDERDCPEISSSGNFS----DENIIVKCKDTEFQCLSR-----------------------NHCIHRTWLCDKDTDCP---DGSDESVQQCGTRNKCRTDQFKCVNGDCIPGHLQCSGAPECIDNSDEENCNFANGE---SSLGCDADKEFDCVGDGRMCVPLEQVCDKMNNCGNWADE 325 HSP 3 Score: 86.6557 bits (213), Expect = 2.857e-18 Identity = 81/245 (33.06%), Postives = 107/245 (43.67%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCD-PNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDE---ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC-------------FAVECDYWQFKCRISGLCIDTRRRCDGTNDC--GSDEDEQDC 270
+ C T F C++ CI + RC+G DC D SDE C + +++F C+ RC+ ++ +CDG C D +DE D C CD ++ C N +CI R +CD DC D SDE + CP + C + +F C D CI K RCDG DC D SDE DC V+C +F+C CI CD DC GSDE Q C
Sbjct: 24 SHCLTTKFECDNKQCIPQSWRCNGLDDCMDNSDEVNCIVKSNCTDSEFRCDEIRCVPKNWQCDGGEDCIDGSDE------------------------NEDICSNQTCDRTCISNEFTCDN-SKCIQNRWVCDQEDDCGDGSDEKS--CPDMSCNADNEFSCGDGY-CIKKYWRCDGDVDCPDASDERDCPEISSSGNFSDENIIVKCKDTEFQCLSRNHCIHRTWLCDKDTDCPDGSDESVQQC 240 HSP 4 Score: 68.9366 bits (167), Expect = 1.268e-12 Identity = 91/302 (30.13%), Postives = 132/302 (43.71%), Query Frame = 0
Query: 155 TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEND--CFAVECDYW----QFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENEC-RLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLD-GSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGS 448
T++EC N ++CI + C+G DC D SDE K C + +F+C D+ RC+ K +CDG DC+D SDEN+ C CD +F C S CI R CD +DCG DE+ C +++ N + NE + I+ D CP S E +C + + SD+ I + C ++QCL CI+ CD+ DC DG DE ++ + C D+F C +G C P +C G +C D SDE +C +
Sbjct: 29 TKFECDN-KQCIPQSWRCNGLDDCMDNSDE-VNCIVKSNCTDS-EFRC-DEIRCVPKNWQCDGGEDCIDGSDENEDICSNQTCDRTCISNEFTCDNSK-CIQNRWVCDQEDDCGDGSDEKSCPDMSCN---ADNEF-----SCGDGYCIKKYWRCDGDVDCPDASD----------ERDCPEISSSGNFSDENIIV----------------KCKDTEFQCLSRNHCIHRTWLCDKDTDCPDGSDE------SVQQCGTRNKC-RTDQFKCVNGDCIPGHLQCSGAPECIDNSDEENCNFAN 284 HSP 5 Score: 57.3806 bits (137), Expect = 5.550e-9 Identity = 38/90 (42.22%), Postives = 49/90 (54.44%), Query Frame = 0
Query: 361 CAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTS 450
C N++ C + CI + CD++ DC DG DEK C +C+ +EF+C DG C RCDG VDC D SDE DCP S+S
Sbjct: 111 CISNEFTCDNSKCIQNRWVCDQEDDCGDGSDEKSC---------PDMSCNADNEFSCGDGYCIKKYWRCDGDVDCPDASDERDCPEISSS 191
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. L. salmonis genes
Match: EMLSAG00000011642 (supercontig:LSalAtl2s:LSalAtl2s80:264857:278656:-1 gene:EMLSAG00000011642 transcript:EMLSAT00000011642 description:"maker-LSalAtl2s80-augustus-gene-2.9") HSP 1 Score: 87.8113 bits (216), Expect = 3.057e-18 Identity = 93/279 (33.33%), Postives = 116/279 (41.58%), Query Frame = 0
Query: 2 RQEVKRKIFIVEYFLVFRGKMSAVATRALNSSEGLMPQDDIPEAQCSRTDFICEDLACIN--FALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCP--KVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN-DCFAVECDYWQFKCRISGL--------CIDTRRRCDGTNDC--GSDE 265
R EV RK FIVE ++ R N + E + C+D CIN + +RCD DC D SDE C SC+P F C NG C+ RCDG C D +DE GC PG C+ Q++C +CI+ R CD DCPD SDE CP K C + +F+C D CI+ R CDG DC DE + +F C+ L CI + CDG DC GSDE
Sbjct: 1406 RIEVGRKRFIVEGLEGGHNYDVQISARVENCYSKQCLKKSKKEEVFLECAYRCDDGTCINKKWDVRCDFIQDCIDGSDERNC--SCNPPKHFQCTNGYCLESWKRCDGKADCNDMSDEAGC-------PG----------------CLAN------QFQCRKSGKCIELSRTCDRKLDCPDGSDEHD--CPYWKKSCWSD-EFRCLDAT-CINSRFVCDGIWDCSGGEDERSCDKSSCSSSSEFTCKPRNLHFPHKESICIPLHKYCDGEKDCVDGSDE 1649 HSP 2 Score: 53.1434 bits (126), Expect = 2.125e-7 Identity = 34/82 (41.46%), Postives = 41/82 (50.00%), Query Frame = 0
Query: 366 WQCLDGSCIYLKQ--QCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCP 445
++C DG+CI K +CD DC DG DE+ CS C+P F C +G C RCDG DC D SDE CP
Sbjct: 1456 YRCDDGTCINKKWDVRCDFIQDCIDGSDERNCS------------CNPPKHFQCTNGYCLESWKRCDGKADCNDMSDEAGCP 1525 HSP 3 Score: 51.9878 bits (123), Expect = 4.544e-7 Identity = 35/83 (42.17%), Postives = 39/83 (46.99%), Query Frame = 0
Query: 360 GCAVNQWQCL-DGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDE 441
GC NQ+QC G CI L + CDRK DC DG DE C P DEF C D +C CDG+ DC DE
Sbjct: 1526 GCLANQFQCRKSGKCIELSRTCDRKLDCPDGSDEHDC--------PYWKKSCWSDEFRCLDATCINSRFVCDGIWDCSGGEDE 1600
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. L. salmonis genes
Match: EMLSAG00000011173 (supercontig:LSalAtl2s:LSalAtl2s760:102949:107319:-1 gene:EMLSAG00000011173 transcript:EMLSAT00000011173 description:"maker-LSalAtl2s760-snap-gene-1.19") HSP 1 Score: 78.9518 bits (193), Expect = 1.713e-15 Identity = 49/146 (33.56%), Postives = 68/146 (46.58%), Query Frame = 0
Query: 89 TQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE 234
+ F C NG CI +CDG +C + DE C ++ E T ++C + C+ K +CDG+ DC D SDE C K++CG+ + C DK C+ +CDGY DCLD SDE C +VE
Sbjct: 971 SLFHCGNGECIPHRWKCDGIAECSNKMDESDCPSVVTC--------------------------EGTNFQCIKSKDCVPKLWVCDGFKDCEDGSDE--HQCQKIDCGQH-KTLCDDKSTCVLDLWKCDGYPDCLDGSDELRCMSVE 1087 HSP 2 Score: 71.2478 bits (173), Expect = 4.802e-13 Identity = 94/323 (29.10%), Postives = 124/323 (38.39%), Query Frame = 0
Query: 138 IIGLTFDPCVPGEC-----DEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVEC-GEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN---------DCFAVECDYWQFKCRISGLCIDTRRRCDGTNDC--GSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLD-EFTCKDGS-CRPIDTRCDGMVDCEDESDE 441
IG+ P + G+ DEE+ C RCI + CDG DC D++DE E C + C G+EW F+C D + CI CDG DC D SDE+ + C+ F C GLCI + CD +DC GSDE EN C +RP+ G + EN ++ + SSD + Y C+ E C +N+W C D C+ +TI EI CDP F CKD + C CDG VDC + DE
Sbjct: 8 FIGILIVPIISGKYIPCHGDEESHL-CLYDARCIPSSKRCDGVIDCDDKTDE--EFCEFITCTGDEW-FKCHDGE-CITSSFVCDGQFDCTDRSDESQSCQSSVSLNNKHSHCEEGDFHCH-DGLCIPSEWTCDKAHDCLDGSDE-------FVEN--------------------------------CAGSNRPIIGKNASKIENT-DVQNRNVSSDTNCEGYL---CKNGE-------CILNRWVCDD-----------------------VCNEKTIAEIE----CDPFKGHFYCKDSNECIIASNVCDGHVDCSNAYDE 247 HSP 3 Score: 65.0846 bits (157), Expect = 3.366e-11 Identity = 42/125 (33.60%), Postives = 60/125 (48.00%), Query Frame = 0
Query: 154 ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPF 278
++ + C N CI R CDG +C ++ DE CP V E FQC + C+ K CDG+ DC D SDE+ C ++C + C C+ +CDG DC DE C ++ + F
Sbjct: 970 DSLFHCGN-GECIPHRWKCDGIAECSNKMDESD--CPSVVTCEGTNFQCIKSKDCVPKLWVCDGFKDCEDGSDEHQCQKIDCGQHKTLCDDKSTCVLDLWKCDGYPDCLDGSDELRCMSVEDTAF 1091 HSP 4 Score: 60.8474 bits (146), Expect = 7.340e-10 Identity = 57/189 (30.16%), Postives = 78/189 (41.27%), Query Frame = 0
Query: 52 FICEDLACINFALRCDGKFDCEDRSDEYQ-CPPSCDPNTQ--------FTCNNGRCIGQDLRCDGTPQCQDSTDEI--GCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEND 229
F C D CI + CDG+FDC DRSDE Q C S N + F C++G CI + CD C D +DE C ++ G N I T D + + CI R +CD C +++ + E P + F C+D CI CDG+ DC + DE +
Sbjct: 72 FKCHDGECITSSFVCDGQFDCTDRSDESQSCQSSVSLNNKHSHCEEGDFHCHDGLCIPSEWTCDKAHDCLDGSDEFVENCAGSNRPIIG----KNASKIENTDVQNRNVSSDTNCEGYLCKNGECILNRWVCDDV--CNEKTIAEIECDP-----FKGHFYCKDSNECIIASNVCDGHVDCSNAYDEGN 249 HSP 5 Score: 60.4622 bits (145), Expect = 9.910e-10 Identity = 70/231 (30.30%), Postives = 95/231 (41.13%), Query Frame = 0
Query: 56 DLACINFALRCDGKFDCEDRSDEYQCP-PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPK----VECGEEWQFQCRDKQ-RCIDKRRRCDGY----SDCL-------DESDENDCFAVECDYWQ--FKCRISGLCIDTRRRCDGTNDCGSDEDE 267
D CI + RCDG DC+D++DE C +C + F C++G CI CDG C D +DE S SLN + C EE + C + CI CD DC D SDE E C + + + D Q R + C+GY +C+ D +E +ECD ++ F C+ S CI CDG DC + DE
Sbjct: 36 DARCIPSSKRCDGVIDCDDKTDEEFCEFITCTGDEWFKCHDGECITSSFVCDGQFDCTDRSDESQSCQSS-------VSLNNK----------HSHC-EEGDFHCHD-GLCIPSEWTCDKAHDCLDGSDEFVENCAGSNRPIIGKNASKIENTDVQNRNVSSDTNCEGYLCKNGECILNRWVCDDVCNEKTIAEIECDPFKGHFYCKDSNECIIASNVCDGHVDCSNAYDE 247
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. L. salmonis genes
Match: EMLSAG00000003726 (supercontig:LSalAtl2s:LSalAtl2s1:3575139:3647958:-1 gene:EMLSAG00000003726 transcript:EMLSAT00000003726 description:"maker-LSalAtl2s1-augustus-gene-36.30") HSP 1 Score: 68.9366 bits (167), Expect = 2.771e-12 Identity = 46/149 (30.87%), Postives = 66/149 (44.30%), Query Frame = 0
Query: 84 SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFA 232
SC+ +F C +GRC+ +C+ C DE CG+IS C +T + C + CI ++ +CDG PDC + DE + C E QF C C+ ++CDG+ DC DESDE DC +
Sbjct: 391 SCNGVFEFKCKSGRCLNISKKCNYVRDCLHGDDEFECGSISN-------------------------CSTKTSFLCLDGT-CISEKNICDGKPDCFSREDEKDCIKYISRC-EYHQFMCLGNLNCVPLSKKCDGFIDCEDESDERDCLS 512 HSP 2 Score: 61.6178 bits (148), Expect = 4.758e-10 Identity = 81/334 (24.25%), Postives = 125/334 (37.43%), Query Frame = 0
Query: 164 RCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC--FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVC--GTDGLTYENECRLKAMSCSSDQEIQIYY------------EGPCETEEDPVEPQG--CAVNQWQCLDGSCIYLKQQCDRKYDCRDGE------------------DEKYCSIETID----------------EIPAPPNCDPLDEFTCKDG-SCRPIDTRCDGMVDCEDESDEFDC 444
RC++ + C+ DC D++ E C + F C D CI ++ CDG DC DE DC + C+Y QF C + C+ ++CDG DC + DE+DC ++ +P + T +S + D+ P + + G+ N RL+ +S + +Y+ E V+ Q C ++ C +G CI CD + DC DG D K C ++T + P + E+ C C P CDG +DC+D SDE C
Sbjct: 404 RCLNISKKCNYVRDCL-HGDDEFECGSISNCSTKTSFLCLDGT-CISEKNICDGKPDCFSREDEKDCIKYISRCEYHQFMCLGNLNCVPLSKKCDGFIDCEDESDERDC--LSHDPHIKI-RLYPTKQTVISGQEVVFQCRDEGRHRLPVVWNKLRDDGSFDRVSSNNGRLEIFE-TSIRHAGVYFCEVDMFTNVSSISATLNVIETQVQSQKFDCQRDEATCSNGQCIPKDNVCDGRRDCFDGSDESSCGGGGGCEPNEYRCDNKRCILKTWVCDSDDDCGDGSDENYCAVNPPGSPCKFKEWMCHSKEQCIPKSFHCDGEIDCQDTSDEIGC 731 HSP 3 Score: 59.3066 bits (142), Expect = 2.241e-9 Identity = 50/172 (29.07%), Postives = 72/172 (41.86%), Query Frame = 0
Query: 51 DFICEDLACINFALRCDGKFDCEDRSDEYQC--PPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDE----------DAEMCP---KVECGEEWQFQCRDKQR 207
+F C+ C+N + +C+ DC DE++C +C T F C +G CI + CDG P C DE C I ++ C E Q+ C + C+ + CDG+ DC D+SDE + P V G+E FQCRD+ R
Sbjct: 397 EFKCKSGRCLNISKKCNYVRDCLHGDDEFECGSISNCSTKTSFLCLDGTCISEKNICDGKPDCFSREDEKDC--IKYIS----------------------RC-EYHQFMCLGNLNCVPLSKKCDGFIDCEDESDERDCLSHDPHIKIRLYPTKQTVISGQEVVFQCRDEGR 543
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. L. salmonis genes
Match: EMLSAG00000002640 (supercontig:LSalAtl2s:LSalAtl2s1533:54194:57280:-1 gene:EMLSAG00000002640 transcript:EMLSAT00000002640 description:"maker-LSalAtl2s1533-augustus-gene-0.8") HSP 1 Score: 65.4698 bits (158), Expect = 1.240e-11 Identity = 69/241 (28.63%), Postives = 93/241 (38.59%), Query Frame = 0
Query: 46 QCSRTDFIC--EDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRR-----CDGTNDCGSDEDEQDCDNITENPFF 279
+C R +C + C + +RCDG DC D +DE QC C+ G CDG C + DE+ CG + C P Q+ CA+ CI CD CPD SDE C E+ F C+ Q C+ +CD DC+D SDE +C LC+D R+ CDG +DC DE +C+ + FF
Sbjct: 14 KCRRGSMVCGLNNTECYSDKVRCDGVVDCSDFADELQC-SGCETFLNMG-YLGISREDKFICDGVFDCPNGEDEMNCGK---------------------NSCSPD------QYYCADSDICIPSIHRCDLKRHCPDGSDEKDCEC------EDGFFTCKFIQECLPMDIKCDFKKDCMDGSDETNC----------------LCVDYLRKNGSQICDGISDCPDSSDELNCETCGSDEFF 203 HSP 2 Score: 59.3066 bits (142), Expect = 1.263e-9 Identity = 58/176 (32.95%), Postives = 79/176 (44.89%), Query Frame = 0
Query: 66 CDGKFDCEDRSDEYQC-PPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSS------LNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDE-DAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAV 233
CDG FDC + DE C SC P+ + ++ CI RCD C D +DE C + GFF+ L I C+ G +ET C ++ R + ++CDG DCPD SDE + E C E F C +CI K++ CD DC + DE C +
Sbjct: 73 CDGVFDCPNGEDEMNCGKNSCSPDQYYCADSDICIPSIHRCDLKRHCPDGSDEKDC----ECEDGFFTCKFIQECLPMDIKCDFKKDCMDGS--DETNCLCVDYLR-KNGSQICDGISDCPDSSDELNCETCGSDE------FFCDLSSKCIPKKKVCDKIIDCKFKEDERHCITL 235 HSP 3 Score: 50.447 bits (119), Expect = 7.535e-7 Identity = 63/236 (26.69%), Postives = 98/236 (41.53%), Query Frame = 0
Query: 161 NHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEKYC 395
N+ C + CDG DC D +DE C E + ++ + CDG DC + DE +C C Q+ C S +CI + RCD C DE+DC+ E+ FF+ I+E + P+ I+ + DC DG N + + + Q + P D + + C +++ C L CI K+ CD+ DC+ EDE++C
Sbjct: 25 NNTECYSDKVRCDGVVDCSDFADE--LQCSGCETFLNMGYLGISRE----DKFICDGVFDCPNGEDEMNCGKNSCSPDQYYCADSDICIPSIHRCDLKRHCPDGSDEKDCE--CEDGFFTCKFIQECL-----PMDIKCDFKKDC-------------MDGSDETNCLCVDYLRKNGSQICDGISDCP--DSSDELNCETCGSDEFFCDLSSKCIPKKKVCDKIIDCKFKEDERHC 232
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. L. salmonis genes
Match: EMLSAG00000004273 (supercontig:LSalAtl2s:LSalAtl2s224:244283:302537:1 gene:EMLSAG00000004273 transcript:EMLSAT00000004273 description:"maker-LSalAtl2s224-augustus-gene-3.16") HSP 1 Score: 62.7734 bits (151), Expect = 2.082e-10 Identity = 68/249 (27.31%), Postives = 91/249 (36.55%), Query Frame = 0
Query: 82 PPSCDPNTQFTCNNG--RCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENEC 328
PP C P+ QFTCN+G CI RCDG P+C D +DE+ C EC Q+ C N + CI+ LCD C D SDE + C QFQC + CI + C+ +SDC D SDE +C+ G+D+ D + + P +N + V +SI V +S C L ENE
Sbjct: 1286 PPXCQPD-QFTCNSGTPSCIPFQWRCDGQPECSDHSDEMDCP----------------------------ECGV-NQFRCQNGQ-CINGTNLCDKINHCSDNSDE-------LNCCLGNQFQCVLSKECIPRNMTCNSFSDCSDGSDE-----------------------MLPQCNVVGSEGNDDSSMDSNMMLTKPSVPSNTSTYLIVIFVGLMSILVF----------GLSVYYCKRKSLAVENEV 1463 HSP 2 Score: 61.6178 bits (148), Expect = 4.657e-10 Identity = 44/135 (32.59%), Postives = 58/135 (42.96%), Query Frame = 0
Query: 58 ACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKV 192
+CI F RCDG+ +C D SDE CP C N QF C NG+CI CD C D++DE+ C C+ Q++C + CI + C+ + DC D SDE C V
Sbjct: 1302 SCIPFQWRCDGQPECSDHSDEMDCP-ECGVN-QFRCQNGQCINGTNLCDKINHCSDNSDELNC-------------------------CL------GNQFQCVLSKECIPRNMTCNSFSDCSDGSDEMLPQCNVV 1403
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. SwissProt
Match: gi|81867523|sp|Q91ZX7.1|LRP1_MOUSE (RecName: Full=Prolow-density lipoprotein receptor-related protein 1; Short=LRP-1; AltName: Full=Alpha-2-macroglobulin receptor; Short=A2MR; AltName: CD_antigen=CD91; Contains: RecName: Full=Low-density lipoprotein receptor-related protein 1 85 kDa subunit; Short=LRP-85; Contains: RecName: Full=Low-density lipoprotein receptor-related protein 1 515 kDa subunit; Short=LRP-515; Contains: RecName: Full=Low-density lipoprotein receptor-related protein 1 intracellular domain; Short=LRPICD; Flags: Precursor) HSP 1 Score: 150.214 bits (378), Expect = 8.397e-37 Identity = 131/417 (31.41%), Postives = 186/417 (44.60%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDE------YQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE-NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCG--SDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKP 452
+ C+ + F+C++ CI F +CD + DC D SDE ++C P QF C+ G C CDG CQD++DE C C+P +Q++C N RCI C+G +C D DE CP+V C QFQC +RCI + CD +DC+D SDE +C + C +F+C+ SG CI R +CDG +DCG SDE +++CD T P+ F +R V G Y+N+C ++E+ P+ C+ +++ C +G CI + +CD +DC DG DEK C+ P CD +D+F CK G C P+ RCD DC D SDE C G + P
Sbjct: 3333 SNCTASQFVCKNDKCIPFWWKCDTEDDCGDHSDEPPDCPEFKCRPG-----QFQCSTGICTNPAFICDGDNDCQDNSDEANC---------------------DIHVCLP------SQFKCTNTNRCIPGIFRCNGQDNCGDGEDE--RDCPEVTCAPN-QFQCSITKRCIPRVWVCDRDNDCVDGSDEPANCTQMTCGVDEFRCKDSGRCIPARWKCDGEDDCGDGSDEPKEECDERTCEPY----------------------------QFRCKNNRCVPGRWQCDYDNDCGDN-------------------SDEESCTPRPCSESEFSCANGRCIAGRWKCDGDHDCADGSDEKDCT----------PRCD-MDQFQCKSGHCIPLRWRCDADADCMDGSDEEACGTGVRTCP 3656 HSP 2 Score: 134.035 bits (336), Expect = 2.225e-31 Identity = 138/433 (31.87%), Postives = 191/433 (44.11%), Query Frame = 0
Query: 41 DIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC-NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCV-----PGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEED-----PVEPQGCAV------NQWQCL-DGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPL 446
D PE +C F C C N A CDG DC+D SDE C +QF C N RCI RC+G C D DE C ++ F S+ R I + + CV P C + T ++ C + RCI R CDG DC D SDE E C + C E +QF+C++ RC+ R +CD +DC D SDE C C +F C +G CI R +CDG +DC DE+DC P ++ + + P+ + DC CGT T C L C++ + ++ C+ E+D P+ CA ++C D C+++ +QCD +C DG DE+ C T P+C EF C++ C RC+ DC D SDE DC +
Sbjct: 3369 DCPEFKCRPGQFQCSTGICTNPAFICDGDNDCQDNSDEANCDIHVCLPSQFKCTNTNRCIPGIFRCNGQDNCGDGEDERDCPEVTCAPNQFQCSITKRCIPRVWVCDRDNDCVDGSDEPANCTQMTCGVDEFRCKDSGRCIPARWKCDGEDDCGDGSDEPKEECDERTC-EPYQFRCKN-NRCVPGRWQCDYDNDCGDNSDEESCTPRPCSESEFSC-ANGRCIAGRWKCDGDHDCADGSDEKDC-----TPRCDMDQFQCKSGHCI-PLRWRCDADADC---MDGSDEEACGTGVRT----CPLDEFQCNNTLCKPLAWK--CDGEDDCGDNSDENPEECARFICPPNRPFRCKNDRVCLWIGRQCDGVDNCGDGTDEEDCEPPTAQN----PHCKDKKEFLCRNQRCLSSSLRCNMFDDCGDGSDEEDCSI 3779 HSP 3 Score: 114.005 bits (284), Expect = 5.368e-25 Identity = 91/242 (37.60%), Postives = 111/242 (45.87%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQ--CPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMC------PKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--CD-YWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENP 277
C F C CI + CD DC DRSDE P+C P TQFTCNNGRCI + RCD C D++DE GC TQ++C N RCI + CDG DC D SDE C P C + +FQCR CI R RCDG +DC+D SDE C V CD +F C+ S CI CDG +DC + DE++C+ + P
Sbjct: 937 CPPNQFSCASGRCIPISWTCDLDDDCGDRSDESASCAYPTCFPLTQFTCNNGRCININWRCDNDNDCGDNSDEAGCSHSC----------------------------SSTQFKC-NSGRCIPEHWTCDGDNDCGDYSDETHANCTNQATRPPGGCHSD-EFQCRLDGLCIPLRWRCDGDTDCMDSSDEKSCEGVTHVCDPNVKFGCKDSARCISKAWVCDGDSDCEDNSDEENCEALACRP 1148 HSP 4 Score: 110.923 bits (276), Expect = 5.720e-24 Identity = 121/392 (30.87%), Postives = 155/392 (39.54%), Query Frame = 0
Query: 78 EYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN------DCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCE--TEEDPVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGS-CRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEP 455
Y PP C P +F C N RCI + +CDG C D++DE P C + T +++C N+R CI R LCDG DC + DE C C QF C RCI CD DC D SDE+ CF + QF C +G CI+ RCD NDCG + DE C + + F N S +PE +C D + Y + T + P GC +++QC LDG CI L+ +CD DC D DEK C E + + CDP +F CKD + C CDG DCED SDE +C + PS P
Sbjct: 848 SYVPPPQCQPG-EFACANNRCIQERWKCDGDNDCLDNSDE-----------------------------APALCHQHTCPSDRFKCENNR-CIPNRWLCDGDNDCGNSEDESNATCSARTCPPN-QFSCASG-RCIPISWTCDLDDDCGDRSDESASCAYPTCFPLT----QFTCN-NGRCININWRCDNDNDCGDNSDEAGCSHSCSSTQFKCN-------------SGRCIPEH-----------------------------WTCDGDNDCGDYSDETHANCTNQATRPPGGCHSDEFQCRLDGLCIPLRWRCDGDTDCMDSSDEKSC--EGVTHV-----CDPNVKFGCKDSARCISKAWVCDGDSDCEDNSDEENCEALACRPPSHP 1152 HSP 5 Score: 105.916 bits (263), Expect = 2.394e-22 Identity = 90/264 (34.09%), Postives = 117/264 (44.32%), Query Frame = 0
Query: 42 IPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCD----PNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFD---------------PCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDED--AEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--------CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITE 275
+P QC +F C + CI +CDG DC D SDE P C P+ +F C N RCI CDG C +S DE ++ P FS +GR I +++ C C TQ+ C N+ RCI+ CD DC D SDE + C QF+C + RCI + CDG +DC D SDE C +F+CR+ GLCI R RCDG DC DE+ C+ +T
Sbjct: 850 VPPPQCQPGEFACANNRCIQERWKCDGDNDCLDNSDE--APALCHQHTCPSDRFKCENNRCIPNRWLCDGDNDCGNSEDESNATCSARTCPPNQFSCASGRCIPISWTCDLDDDCGDRSDESASCAYPTCFPLTQFTC-NNGRCININWRCDNDNDCGDNSDEAGCSHSCSST------QFKC-NSGRCIPEHWTCDGDNDCGDYSDETHANCTNQATRPPGGCHSDEFQCRLDGLCIPLRWRCDGDTDCMDSSDEKSCEGVTH 1103 HSP 6 Score: 95.1301 bits (235), Expect = 6.310e-19 Identity = 111/374 (29.68%), Postives = 143/374 (38.24%), Query Frame = 0
Query: 84 SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVEC-DYWQFK--------CRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYY----EGPCETEEDPVE-PQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDE-KYCSIETIDEIPAPPNCDPLDEFTC-KDGSCRPIDTRCDGMVDCEDESDE 441
SC +F C NG CI L CDG C+D +DE P + N R TF C N+ RC+ C+G DC D SDE C K CG +F+CRD CI RC+ + DC D SDE +C A +C Y++ C + LC CDG NDCG DE+D CP + RP C L +C S + I + + E CE ED + C+ Q++C + CI + CD DC DG DE +C +T C P F+C C P CDG DC D +DE
Sbjct: 2524 SCRAQDEFECANGECISFSLTCDGVSHCKDKSDE---------KPSY---CNSRRCKKTFRQC--------------NNGRCVSNMLWCNGVDDCGDGSDEIP--CNKTACGVG-EFRCRDGS-CIGNSSRCNQFVDCEDASDEMNCSATDCSSYFRLGVKGVLFQPCERTSLCYAPSWVCDGANDCGDYSDERD---------------------------------------CPGVKRP-----------RCPLNYFACPSGRCIPMSWTCDKEDDCENGEDETHCNKFCSEAQFECQNHRCISKQWLCDGSDDCGDGSDEAAHCEGKT---------CGP-SSFSCPGTHVCVPERWLCDGDKDCTDGADE 2807 HSP 7 Score: 94.7449 bits (234), Expect = 8.267e-19 Identity = 121/422 (28.67%), Postives = 170/422 (40.28%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVE---CGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDE-QDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYY---------EGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQ-QCDRKYDCRDGEDEKYCSIETIDEIPAPPN-------CDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPL 446
+C +T C + C++ L C+G DC D SDE C + +F C +G CIG RC+ C+D++DE+ C A S + G+ F PC E T C +CDG DC D SDE CP V+ C + F C RCI CD DC + DE C C QF+C+ + CI + CDG++DCG DE C+ T P + P P + DC T G Y + C + C + I ++ +G E+ E E C N+++C +G C+ +Q +CD + DC D DE P P+ C+ +F C G C C+G DC D SDE C +
Sbjct: 2566 RCKKTFRQCNNGRCVSNMLWCNGVDDCGDGSDEIPCNKTACGVGEFRCRDGSCIGNSSRCNQFVDCEDASDEMNCSATD-----CSSYFRLGVKGVLFQPC------ERTS-------LCYAPSWVCDGANDCGDYSDE--RDCPGVKRPRCPLNY-FAC-PSGRCIPMSWTCDKEDDCENGEDETHCNKF-CSEAQFECQ-NHRCISKQWLCDGSDDCGDGSDEAAHCEGKTCGP----SSFSCPGTHVCVPERWLCDGDKDCTDGADESV-----TAGCLYNSTCDDREFMCQNRLCIPKHFVCDHDRDCADGSDESPE--CEYPTCGPNEFRCANGRCLSSRQWECDGENDCHDHSDEA----------PKNPHCTSPEHKCNASSQFLCSSGRCVAEALLCNGQDDCGDGSDERGCHV 2942 HSP 8 Score: 94.3597 bits (233), Expect = 1.083e-18 Identity = 81/254 (31.89%), Postives = 118/254 (46.46%), Query Frame = 0
Query: 38 PQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGEC----DEET-----------QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVEC------DYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
P+++ E C F C++ C+ +CD DC D SDE C P ++F+C NGRCI +CDG C D +DE C + F +G I L + +C DEE +++C N+ C CDG DC D SDE+ E C + C F+C++ + C+ R+CDG +C D +DE DC D +F CR + C+ + RC+ +DCG DE+DC
Sbjct: 3528 PKEECDERTCEPYQFRCKNNRCVPGRWQCDYDNDCGDNSDEESCTPRPCSESEFSCANGRCIAGRWKCDGDHDCADGSDEKDC--TPRCDMDQFQCKSGHCIPLRWRCDADADCMDGSDEEACGTGVRTCPLDEFQC-NNTLCKPLAWKCDGEDDCGDNSDENPEECARFICPPNRPFRCKNDRVCLWIGRQCDGVDNCGDGTDEEDCEPPTAQNPHCKDKKEFLCR-NQRCLSSSLRCNMFDDCGDGSDEEDC 3777 HSP 9 Score: 83.5741 bits (205), Expect = 2.574e-15 Identity = 85/260 (32.69%), Postives = 111/260 (42.69%), Query Frame = 0
Query: 42 IPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIG------CGAISKVAPGFFSSLNGRII-------------GLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRR-RCDGYSDCLDESD-----------ENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
+ +C F C CI + CD + DCE+ DE C C QF C N RCI + CDG+ C D +DE CG S PG + R + +T CD+ ++ C N R CI K +CD DC D SDE E C CG +F+C + RC+ R+ CDG +DC D SD E+ C A QF C SG C+ C+G +DCG DE+ C
Sbjct: 2692 VKRPRCPLNYFACPSGRCIPMSWTCDKEDDCENGEDETHCNKFCS-EAQFECQNHRCISKQWLCDGSDDCGDGSDEAAHCEGKTCGPSSFSCPGTHVCVPERWLCDGDKDCTDGADESVTAGCLYNSTCDDR-EFMCQN-RLCIPKHFVCDHDRDCADGSDESPE-CEYPTCGPN-EFRCANG-RCLSSRQWECDGENDCHDHSDEAPKNPHCTSPEHKCNASS----QFLCS-SGRCVAEALLCNGQDDCGDGSDERGC 2940 HSP 10 Score: 82.0333 bits (201), Expect = 6.599e-15 Identity = 91/325 (28.00%), Postives = 127/325 (39.08%), Query Frame = 0
Query: 136 GRIIGLTFD-PCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYE-----NECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSE 454
GRI+ F V C + ++ECAN CI CDG C D+SDE C C + ++ QC + RC+ C+G DC D SDE C C +F+CR G CI RC+ DC DE +C T C S R G G+ ++ + C + C + Y + E + V+ C +N + C G CI + CD++ DC +GEDE +C+ + +F C++ C CDG DC D SDE G T PS
Sbjct: 2509 GRILQEDFTCRAVNSSCRAQDEFECANGE-CISFSLTCDGVSHCKDKSDEKPSYCNSRRCKKTFR-QC-NNGRCVSNMLWCNGVDDCGDGSDEIPCNKTACGVGEFRCR-DGSCIGNSSRCNQFVDCEDASDEMNCS----------------------------------ATDCSSYFR--LGVKGVLFQPCERTSLCYAPSWVCDGANDCGDYSD---ERDCPGVKRPRCPLNYFACPSGRCIPMSWTCDKEDDCENGEDETHCNKFCSEA-----------QFECQNHRCISKQWLCDGSDDCGDGSDEAAHCEGKTCGPSS 2779 HSP 11 Score: 62.003 bits (149), Expect = 9.958e-9 Identity = 83/275 (30.18%), Postives = 110/275 (40.00%), Query Frame = 0
Query: 199 QFQCRDKQRCIDKRRRCDGYSDCLDESDEN-DCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPL----DEFTCKD-GSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEPEPTTADPFEYE 466
QF C++ +CI +CD DC D SDE DC +C QF+C +G+C + CDG NDC + DE +CD I +P+ C + +R + G ++ C G E E D E CA NQ+QC + CI CDR DC DG DE P NC + DEF CKD G C P +CDG DC D SDE E + T +P+++
Sbjct: 3339 QFVCKN-DKCIPFWWKCDTEDDCGDHSDEPPDCPEFKCRPGQFQCS-TGICTNPAFICDGDNDCQDNSDEANCD------------IHVCLPSQFK---------------CTNTNRCIPGIFRCNGQDNC------------------GDGEDERDCPEVT-CAPNQFQCSITKRCIPRVWVCDRDNDCVDGSDE-------------PANCTQMTCGVDEFRCKDSGRCIPARWKCDGEDDCGDGSDE---------PKEECDERTCEPYQFR 3543
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. SwissProt
Match: gi|317373384|sp|Q07954.2|LRP1_HUMAN (RecName: Full=Prolow-density lipoprotein receptor-related protein 1; Short=LRP-1; AltName: Full=Alpha-2-macroglobulin receptor; Short=A2MR; AltName: Full=Apolipoprotein E receptor; Short=APOER; AltName: CD_antigen=CD91; Contains: RecName: Full=Low-density lipoprotein receptor-related protein 1 85 kDa subunit; Short=LRP-85; Contains: RecName: Full=Low-density lipoprotein receptor-related protein 1 515 kDa subunit; Short=LRP-515; Contains: RecName: Full=Low-density lipoprotein receptor-related protein 1 intracellular domain; Short=LRPICD; Flags: Precursor) HSP 1 Score: 150.214 bits (378), Expect = 8.870e-37 Identity = 131/417 (31.41%), Postives = 186/417 (44.60%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDE------YQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE-NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCG--SDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKP 452
+ C+ + F+C++ CI F +CD + DC D SDE ++C P QF C+ G C CDG CQD++DE C C+P +Q++C N RCI C+G +C D DE CP+V C QFQC +RCI + CD +DC+D SDE +C + C +F+C+ SG CI R +CDG +DCG SDE +++CD T P+ F +R V G Y+N+C ++E+ P+ C+ +++ C +G CI + +CD +DC DG DEK C+ P CD +D+F CK G C P+ RCD DC D SDE C G + P
Sbjct: 3332 SNCTASQFVCKNDKCIPFWWKCDTEDDCGDHSDEPPDCPEFKCRPG-----QFQCSTGICTNPAFICDGDNDCQDNSDEANC---------------------DIHVCLP------SQFKCTNTNRCIPGIFRCNGQDNCGDGEDE--RDCPEVTCAPN-QFQCSITKRCIPRVWVCDRDNDCVDGSDEPANCTQMTCGVDEFRCKDSGRCIPARWKCDGEDDCGDGSDEPKEECDERTCEPY----------------------------QFRCKNNRCVPGRWQCDYDNDCGDN-------------------SDEESCTPRPCSESEFSCANGRCIAGRWKCDGDHDCADGSDEKDCT----------PRCD-MDQFQCKSGHCIPLRWRCDADADCMDGSDEEACGTGVRTCP 3655 HSP 2 Score: 131.339 bits (329), Expect = 1.427e-30 Identity = 137/433 (31.64%), Postives = 190/433 (43.88%), Query Frame = 0
Query: 41 DIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC-NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCV-----PGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEED-----PVEPQGCAV------NQWQCL-DGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPL 446
D PE +C F C C N A CDG DC+D SDE C +QF C N RCI RC+G C D DE C ++ F S+ R I + + CV P C + T ++ C + RCI R CDG DC D SDE E C + C E +QF+C++ RC+ R +CD +DC D SDE C C +F C +G CI R +CDG +DC DE+DC P ++ + + P+ + DC CGT T C L C++ + ++ C+ E+D P+ CA ++C D C+++ +QCD +C DG DE+ C T +C EF C++ C RC+ DC D SDE DC +
Sbjct: 3368 DCPEFKCRPGQFQCSTGICTNPAFICDGDNDCQDNSDEANCDIHVCLPSQFKCTNTNRCIPGIFRCNGQDNCGDGEDERDCPEVTCAPNQFQCSITKRCIPRVWVCDRDNDCVDGSDEPANCTQMTCGVDEFRCKDSGRCIPARWKCDGEDDCGDGSDEPKEECDERTC-EPYQFRCKN-NRCVPGRWQCDYDNDCGDNSDEESCTPRPCSESEFSC-ANGRCIAGRWKCDGDHDCADGSDEKDC-----TPRCDMDQFQCKSGHCI-PLRWRCDADADC---MDGSDEEACGTGVRT----CPLDEFQCNNTLCKPLAWK--CDGEDDCGDNSDENPEECARFVCPPNRPFRCKNDRVCLWIGRQCDGTDNCGDGTDEEDCEPPTAHTT----HCKDKKEFLCRNQRCLSSSLRCNMFDDCGDGSDEEDCSI 3778 HSP 3 Score: 116.316 bits (290), Expect = 9.323e-26 Identity = 92/242 (38.02%), Postives = 112/242 (46.28%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQ--CPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMC------PKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--CD-YWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENP 277
C F C CI + CD DC DRSDE P+C P TQFTCNNGRCI + RCD C D++DE GC TQ++C N RCI + CDG DC D SDE C P C + +FQCR CI R RCDG +DC+D SDE C V CD +F C+ S CI CDG NDC + DE++C+++ P
Sbjct: 936 CPPNQFSCASGRCIPISWTCDLDDDCGDRSDESASCAYPTCFPLTQFTCNNGRCININWRCDNDNDCGDNSDEAGCSHSC----------------------------SSTQFKC-NSGRCIPEHWTCDGDNDCGDYSDETHANCTNQATRPPGGCHTD-EFQCRLDGLCIPLRWRCDGDTDCMDSSDEKSCEGVTHVCDPSVKFGCKDSARCISKAWVCDGDNDCEDNSDEENCESLACRP 1147 HSP 4 Score: 110.538 bits (275), Expect = 6.532e-24 Identity = 121/392 (30.87%), Postives = 155/392 (39.54%), Query Frame = 0
Query: 78 EYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN------DCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCE--TEEDPVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGS-CRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEP 455
Y PP C P +F C N RCI + +CDG C D++DE P C + T +++C N+R CI R LCDG DC + DE C C QF C RCI CD DC D SDE+ CF + QF C +G CI+ RCD NDCG + DE C + + F N S +PE +C D + Y + T + P GC +++QC LDG CI L+ +CD DC D DEK C E + + CDP +F CKD + C CDG DCED SDE +C + PS P
Sbjct: 847 SYVPPPQCQPG-EFACANSRCIQERWKCDGDNDCLDNSDE-----------------------------APALCHQHTCPSDRFKCENNR-CIPNRWLCDGDNDCGNSEDESNATCSARTCPPN-QFSCASG-RCIPISWTCDLDDDCGDRSDESASCAYPTCFPLT----QFTCN-NGRCININWRCDNDNDCGDNSDEAGCSHSCSSTQFKCN-------------SGRCIPEH-----------------------------WTCDGDNDCGDYSDETHANCTNQATRPPGGCHTDEFQCRLDGLCIPLRWRCDGDTDCMDSSDEKSC--EGVTHV-----CDPSVKFGCKDSARCISKAWVCDGDNDCEDNSDEENCESLACRPPSHP 1151 HSP 5 Score: 106.301 bits (264), Expect = 1.854e-22 Identity = 90/264 (34.09%), Postives = 117/264 (44.32%), Query Frame = 0
Query: 42 IPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCD----PNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFD---------------PCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDED--AEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--------CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITE 275
+P QC +F C + CI +CDG DC D SDE P C P+ +F C N RCI CDG C +S DE ++ P FS +GR I +++ C C TQ+ C N+ RCI+ CD DC D SDE + C QF+C + RCI + CDG +DC D SDE C +F+CR+ GLCI R RCDG DC DE+ C+ +T
Sbjct: 849 VPPPQCQPGEFACANSRCIQERWKCDGDNDCLDNSDE--APALCHQHTCPSDRFKCENNRCIPNRWLCDGDNDCGNSEDESNATCSARTCPPNQFSCASGRCIPISWTCDLDDDCGDRSDESASCAYPTCFPLTQFTC-NNGRCININWRCDNDNDCGDNSDEAGCSHSCSST------QFKC-NSGRCIPEHWTCDGDNDCGDYSDETHANCTNQATRPPGGCHTDEFQCRLDGLCIPLRWRCDGDTDCMDSSDEKSCEGVTH 1102 HSP 6 Score: 96.6709 bits (239), Expect = 1.994e-19 Identity = 112/374 (29.95%), Postives = 142/374 (37.97%), Query Frame = 0
Query: 84 SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVEC-DYWQFK--------CRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYY----EGPCETEEDPVE-PQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDE-KYCSIETIDEIPAPPNCDPLDEFTC-KDGSCRPIDTRCDGMVDCEDESDE 441
SC +F C NG CI L CDG P C+D +DE P + N R TF C G C W C+G DC D SDE C K CG +F+CRD CI RC+ + DC D SDE +C A +C Y++ C + LC CDG NDCG DE+D CP + RP C L +C S + I + + E CE ED + C+ Q++C + CI + CD DC DG DE +C +T C P F+C C P CDG DC D +DE
Sbjct: 2523 SCRAQDEFECANGECINFSLTCDGVPHCKDKSDE---------KPSY---CNSRRCKKTFRQCSNGRCVSNMLW--------------CNGADDCGDGSDEIP--CNKTACGVG-EFRCRDGT-CIGNSSRCNQFVDCEDASDEMNCSATDCSSYFRLGVKGVLFQPCERTSLCYAPSWVCDGANDCGDYSDERD---------------------------------------CPGVKRP-----------RCPLNYFACPSGRCIPMSWTCDKEDDCEHGEDETHCNKFCSEAQFECQNHRCISKQWLCDGSDDCGDGSDEAAHCEGKT---------CGP-SSFSCPGTHVCVPERWLCDGDKDCADGADE 2806 HSP 7 Score: 96.2857 bits (238), Expect = 2.503e-19 Identity = 82/254 (32.28%), Postives = 119/254 (46.85%), Query Frame = 0
Query: 38 PQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGEC----DEET-----------QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC-----FAVEC-DYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
P+++ E C F C++ C+ +CD DC D SDE C P ++F+C NGRCI +CDG C D +DE C + F +G I L + +C DEE +++C N+ C CDG DC D SDE+ E C + C F+C++ + C+ R+CDG +C D +DE DC C D +F CR + C+ + RC+ +DCG DE+DC
Sbjct: 3527 PKEECDERTCEPYQFRCKNNRCVPGRWQCDYDNDCGDNSDEESCTPRPCSESEFSCANGRCIAGRWKCDGDHDCADGSDEKDC--TPRCDMDQFQCKSGHCIPLRWRCDADADCMDGSDEEACGTGVRTCPLDEFQC-NNTLCKPLAWKCDGEDDCGDNSDENPEECARFVCPPNRPFRCKNDRVCLWIGRQCDGTDNCGDGTDEEDCEPPTAHTTHCKDKKEFLCR-NQRCLSSSLRCNMFDDCGDGSDEEDC 3776 HSP 8 Score: 90.8929 bits (224), Expect = 1.351e-17 Identity = 130/461 (28.20%), Postives = 188/461 (40.78%), Query Frame = 0
Query: 35 GLMPQDDIP----EAQCSRTD-FICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDP----NTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEE------TQWECANHRR----------------CIDKRRLCDGYPDCPDQSDEDAEMCPKVE---CGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDE-QDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYY---------EGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQ-QCDRKYDCRDGEDEK----YCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPL 446
G + QDD+ + C D F C + CINF+L CDG C+D+SDE P C+ T C+NGRC+ L C+G C D +DEI C + G F +G IG + +C++ + +C+++ R C +CDG DC D SDE CP V+ C + F C RCI CD DC DE C C QF+C+ + CI + CDG++DCG DE C+ T P + P P + DC C Y + C + C + Q I ++ +G E+ E E C ++++C +G C+ +Q +CD + DC D DE +C+ + C+ +F C G C C+G DC D SDE C +
Sbjct: 2508 GRILQDDLTCRAVNSSCRAQDEFECANGECINFSLTCDGVPHCKDKSDEK--PSYCNSRRCKKTFRQCSNGRCVSNMLWCNGADDCGDGSDEIPCNK-TACGVGEFRCRDGTCIGNSSRCNQFVDCEDASDEMNCSATDCSSYFRLGVKGVLFQPCERTSLCYAPSWVCDGANDCGDYSDE--RDCPGVKRPRCPLNY-FAC-PSGRCIPMSWTCDKEDDCEHGEDETHCNKF-CSEAQFECQ-NHRCISKQWLCDGSDDCGDGSDEAAHCEGKTCGP----SSFSCPGTHVCVPERWLCDGDKDCADGADESIAAGC-----LYNSTCDDREFMCQNRQCIPKHFVCDHDRDCADGSDESPE--CEYPTCGPSEFRCANGRCLSSRQWECDGENDCHDQSDEAPKNPHCTSQ-------EHKCNASSQFLCSSGRCVAEALLCNGQDDCGDSSDERGCHI 2941 HSP 9 Score: 85.8853 bits (211), Expect = 4.187e-16 Identity = 116/429 (27.04%), Postives = 161/429 (37.53%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAI---------------------------SKVAPG------------------------FFSSLNGRIIGLTF-----DPCVPGE----CDE---ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC----FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPC--ETEEDPVEPQGC-------AVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIE 398
+C +T C + C++ L C+G DC D SDE C + +F C +G CIG RC+ C+D++DE+ C A S V G +F+ +GR I +++ D C GE C++ E Q+EC NH RCI K+ LCDG DC D SDE A C CG F C C+ +R CDG DC D +DE+ + CD +F C+ + CI CD DC DE PE + PT PS E R C S ++ + E C +++E P P A +Q+ C G C+ C+ + DC D DE+ C I
Sbjct: 2565 RCKKTFRQCSNGRCVSNMLWCNGADDCGDGSDEIPCNKTACGVGEFRCRDGTCIGNSSRCNQFVDCEDASDEMNCSATDCSSYFRLGVKGVLFQPCERTSLCYAPSWVCDGANDCGDYSDERDCPGVKRPRCPLNYFACPSGRCIPMSWTCDKEDDCEHGEDETHCNKFCSEAQFECQNH-RCISKQWLCDGSDDCGDGSDEAAH-CEGKTCGPS-SFSCPGTHVCVPERWLCDGDKDCADGADESIAAGCLYNSTCDDREFMCQ-NRQCIPKHFVCDHDRDCADGSDES--------------------------------PECEYPTCGPS---------------EFRCANGRCLSSRQWECDGENDCHDQSDEAPKNPHCTSQEHKCNASSQFLCSSGRCVAEALLCNGQDDCGDSSDERGCHIN 2942 HSP 10 Score: 62.003 bits (149), Expect = 1.066e-8 Identity = 83/275 (30.18%), Postives = 110/275 (40.00%), Query Frame = 0
Query: 199 QFQCRDKQRCIDKRRRCDGYSDCLDESDEN-DCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPL----DEFTCKD-GSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEPEPTTADPFEYE 466
QF C++ +CI +CD DC D SDE DC +C QF+C +G+C + CDG NDC + DE +CD I +P+ C + +R + G ++ C G E E D E CA NQ+QC + CI CDR DC DG DE P NC + DEF CKD G C P +CDG DC D SDE E + T +P+++
Sbjct: 3338 QFVCKN-DKCIPFWWKCDTEDDCGDHSDEPPDCPEFKCRPGQFQCS-TGICTNPAFICDGDNDCQDNSDEANCD------------IHVCLPSQFK---------------CTNTNRCIPGIFRCNGQDNC------------------GDGEDERDCPEVT-CAPNQFQCSITKRCIPRVWVCDRDNDCVDGSDE-------------PANCTQMTCGVDEFRCKDSGRCIPARWKCDGEDDCGDGSDE---------PKEECDERTCEPYQFR 3542
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. SwissProt
Match: gi|1708864|sp|P98157.1|LRP1_CHICK (RecName: Full=Low-density lipoprotein receptor-related protein 1; Short=LRP-1; AltName: Full=Alpha-2-macroglobulin receptor; Short=A2MR; Flags: Precursor) HSP 1 Score: 150.214 bits (378), Expect = 9.631e-37 Identity = 132/413 (31.96%), Postives = 189/413 (45.76%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQ-CPP-SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE-NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCG--SDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKP 452
+ C+ + F+C++ CI F +CD + DC DRSDE + CP C P QF C+ G C CDG CQD++DE C C+P +Q++C N RCI C+G +C D DE + CP+V C QFQC +RCI + CD +DC+D SDE +C + C +F+C+ SG CI R +CDG +DCG SDE +++CD T P+ F +R V G Y+N+C + +E+ P+ C+ +++ C +G CI + +CD +DC DG DEK C P C+ D++ CK+G C P+ RCD DC D +DE DC G + P
Sbjct: 3329 SNCTASQFVCKNDKCIPFWWKCDTEDDCGDRSDEPEDCPEFKCRPG-QFQCSTGICTNPAFICDGDNDCQDNSDEANC---------------------DIHVCLP------SQFKCTNTNRCIPGIFRCNGQDNCGDGEDE--KDCPEVTCAPN-QFQCAITKRCIPRVWVCDRDNDCVDGSDEPANCTQMTCGVDEFRCKDSGRCIPARWKCDGEDDCGDGSDEPKEECDERTCEPY----------------------------QFRCKNNRCVPGRWQCDYDNDCGDNS-------------------DEESCTPRPCSESEFSCANGRCIAGRWKCDGDHDCADGSDEKDC----------IPRCE-FDQYQCKNGHCIPMRWRCDADADCMDGTDEEDCGTGVRTCP 3652 HSP 2 Score: 119.783 bits (299), Expect = 7.424e-27 Identity = 137/462 (29.65%), Postives = 185/462 (40.04%), Query Frame = 0
Query: 40 DDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNN-GRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCV-----PGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQ--------------------------------------RCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDN-ITENPF--FSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDP-LDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
+D PE +C F C C N A CDG DC+D SDE C +QF C N RCI RC+G C D DE C ++ F ++ R I + + CV P C + T ++ C + RCI R CDG DC D SDE E C + C E +QF+C++ + RCI R +CDG DC D SDE DC C++ Q++C+ +G CI R RCD DC DE+DC + P F N T P++ + EDDC G + EC + ++ P P P C D C+++ +QCD +C D DEK C T P +C +EF C++ C + RC+ DC D SDE C
Sbjct: 3364 EDCPEFKCRPGQFQCSTGICTNPAFICDGDNDCQDNSDEANCDIHVCLPSQFKCTNTNRCIPGIFRCNGQDNCGDGEDEKDCPEVTCAPNQFQCAITKRCIPRVWVCDRDNDCVDGSDEPANCTQMTCGVDEFRCKDSGRCIPARWKCDGEDDCGDGSDEPKEECDERTC-EPYQFRCKNNRCVPGRWQCDYDNDCGDNSDEESCTPRPCSESEFSCANGRCIAGRWKCDGDHDCADGSDEKDCIP-RCEFDQYQCK-NGHCIPMRWRCDADADCMDGTDEEDCGTGVRTCPLDEFQCNN------TLRKPLAWKCDGEDDC------------GDNSDENPEEC--------------LKFQCP------PNRPFRCKN------DRVCLWIGRQCDGIDNCGDNTDEKDCESPT----AKPKSCSQDKNEFLCENKKCISANLRCNFFDDCGDGSDEKSC 3774 HSP 3 Score: 116.316 bits (290), Expect = 1.154e-25 Identity = 98/270 (36.30%), Postives = 121/270 (44.81%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQ--CPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMC------PKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--CD-YWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDC 305
CS F C CI + CD DC DRSDE P+C P TQFTCNNGRCI + RCD C D++DE GC Q++C N RCI CDG DC D SDE C P C + +FQCR CI R RCDG +DC+D SDE +C V CD +F C+ S CI CDG +DC + DE++C+++ P P T + SI + PE C
Sbjct: 934 CSPNQFSCASGRCIPISWTCDLDDDCGDRSDESASCAYPTCFPLTQFTCNNGRCININWRCDNDNDCGDNSDEAGCSHSC----------------------------SSNQFKC-NSGRCIPVHWTCDGDNDCGDYSDETHANCTNQATRPPGGCHTD-EFQCRLDGLCIPMRWRCDGDTDCMDSSDEKNCEGVTHVCDPNVKFGCKDSARCISKAWVCDGDSDCEDNSDEENCESLVCKP---------PSHTCANNTSICLPPEKLC 1164 HSP 4 Score: 107.457 bits (267), Expect = 8.097e-23 Identity = 116/379 (30.61%), Postives = 150/379 (39.58%), Query Frame = 0
Query: 78 EYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN------DCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGS-CRPIDTRCDGMVDCEDESDEFDC 444
Y PP C P +F C N RCI + +CDG C D++DE P C + T +++C N+R CI R LCDG DC + DE C C QF C RCI CD DC D SDE+ CF + QF C +G CI+ RCD NDCG + DE C + + F N P+ ++DC Y +E T + P GC +++QC LDG CI ++ +CD DC D DEK C E + + CDP +F CKD + C CDG DCED SDE +C
Sbjct: 845 SYIPPPQCQPG-EFACKNNRCIQERWKCDGDNDCLDNSDE-----------------------------APELCHQHTCPSDRFKCKNNR-CIPNRWLCDGDNDCGNNEDESNSTCSARTCSPN-QFSCA-SGRCIPISWTCDLDDDCGDRSDESASCAYPTCFPLT----QFTCN-NGRCININWRCDNDNDCGDNSDEAGCSHSCSSNQFKCNS------GRCIPVHWTCDGDNDCGD----------------YSDETHANC------------------TNQATRPPGGCHTDEFQCRLDGLCIPMRWRCDGDTDCMDSSDEKNC--EGVTHV-----CDPNVKFGCKDSARCISKAWVCDGDSDCEDNSDEENC 1138 HSP 5 Score: 106.686 bits (265), Expect = 1.411e-22 Identity = 90/260 (34.62%), Postives = 118/260 (45.38%), Query Frame = 0
Query: 42 IPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEY--QCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFD---------------PCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--------CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITE 275
IP QC +F C++ CI +CDG DC D SDE C P+ +F C N RCI CDG C ++ DE ++ +P FS +GR I +++ C C TQ+ C N+ RCI+ CD DC D SDE C C QF+C + RCI CDG +DC D SDE C +F+CR+ GLCI R RCDG DC DE++C+ +T
Sbjct: 847 IPPPQCQPGEFACKNNRCIQERWKCDGDNDCLDNSDEAPELCHQHTCPSDRFKCKNNRCIPNRWLCDGDNDCGNNEDESNSTCSARTCSPNQFSCASGRCIPISWTCDLDDDCGDRSDESASCAYPTCFPLTQFTC-NNGRCININWRCDNDNDCGDNSDEAG--CSH-SCSSN-QFKC-NSGRCIPVHWTCDGDNDCGDYSDETHANCTNQATRPPGGCHTDEFQCRLDGLCIPMRWRCDGDTDCMDSSDEKNCEGVTH 1100 HSP 6 Score: 98.9821 bits (245), Expect = 3.472e-20 Identity = 119/419 (28.40%), Postives = 162/419 (38.66%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCP---KVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE---CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYY-------EGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQ-QCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDE------FTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
+C + C + C+ C+G DC D SDE C + T+F C +G CIG RC+ C+D++DE+ C A S + G TF +C + C +CDG DC D SDE CP K +C + F C RCI CD DC + DE C + C QF+C + CI CDG +DCG DE +T ST + P P + DC C Y N C + C + Q I ++ G E E C ++++C +G C+ Q +CD ++DC D DE P P C + F CK+G C P CD DC D SDE +C
Sbjct: 2559 KCKKGFLHCMNGRCVASRFWCNGVDDCGDNSDEVPCNKTSCAATEFRCRDGSCIGNSSRCNQFIDCEDASDEMNC-----TATDCSSYFKLGVKGTTFQ-------------KCEHTSLCYAPSWVCDGANDCGDYSDE--RNCPGGRKPKCPANY-FAC-PSGRCIPMTWTCDKEDDCENGEDETHCSERQDKFCYPVQFECN-NHRCISKLWVCDGADDCGDGSDEDSRCRLTT---CSTGSFQCPGTYVCVPERWLCDGDKDCADGADETLAAGC-----LYNNTCDEREFMCGNRQCIPKHFVCDHDDDCGDGSDESPECEYPTCGPHEFRCANGRCLSNSQWECDGEFDCHDHSDEA----------PKNPRCSSPESKCNDSFFMCKNGKCIPEALLCDNNNDCADGSDELNC 2936 HSP 7 Score: 94.7449 bits (234), Expect = 7.067e-19 Identity = 79/257 (30.74%), Postives = 118/257 (45.91%), Query Frame = 0
Query: 38 PQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET---------------QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVEC-------DYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDN 272
P+++ E C F C++ C+ +CD DC D SDE C P ++F+C NGRCI +CDG C D +DE C I + + NG I + + +C + T +++C N R + CDG DC D SDE+ E C K +C F+C++ + C+ R+CDG +C D +DE DC + D +F C + CI RC+ +DCG DE+ C +
Sbjct: 3524 PKEECDERTCEPYQFRCKNNRCVPGRWQCDYDNDCGDNSDEESCTPRPCSESEFSCANGRCIAGRWKCDGDHDCADGSDEKDC--IPRCEFDQYQCKNGHCIPMRWRCDADADCMDGTDEEDCGTGVRTCPLDEFQCNNTLRKPLAWK-CDGEDDCGDNSDENPEECLKFQCPPNRPFRCKNDRVCLWIGRQCDGIDNCGDNTDEKDCESPTAKPKSCSQDKNEFLCE-NKKCISANLRCNFFDDCGDGSDEKSCSH 3776 HSP 8 Score: 87.8113 bits (216), Expect = 1.267e-16 Identity = 80/229 (34.93%), Postives = 104/229 (45.41%), Query Frame = 0
Query: 40 DDIP--EAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC-----------NNGRCIGQDLRCDGTPQCQDSTDEIGC--GAISKVAPGFFSSLNGRIIGLTF-----DPCVPGE----CDEE-------TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDY 237
D++P + C+ T+F C D +CI + RC+ DCED SDE C + D ++ F + C CDG C D +DE C G K +F+ +GR I +T+ D C GE C E Q+EC NH RCI K +CDG DC D SDED+ C C FQC C+ +R CDG DC D +DE A C Y
Sbjct: 2590 DEVPCNKTSCAATEFRCRDGSCIGNSSRCNQFIDCEDASDEMNCTAT-DCSSYFKLGVKGTTFQKCEHTSLCYAPSWVCDGANDCGDYSDERNCPGGRKPKCPANYFACPSGRCIPMTWTCDKEDDCENGEDETHCSERQDKFCYPVQFECNNH-RCISKLWVCDGADDCGDGSDEDSR-CRLTTCSTG-SFQCPGTYVCVPERWLCDGDKDCADGADET--LAAGCLY 2812 HSP 9 Score: 86.6557 bits (213), Expect = 2.578e-16 Identity = 117/411 (28.47%), Postives = 148/411 (36.01%), Query Frame = 0
Query: 84 SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAIS-KVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVEC-DYWQF--------KCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDC-----PTFCPSMSRPVCGTDGLTYENE-CRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDG-SCIYLKQQCDRKYDCRDGEDEKYCS----IETIDE----------IPAP---------------------PNCDPLDEFTCKDGSCRP-IDTRCDGMVDCEDESDE 441
+C+ + +F C NG CI CDG C+D +DE S K GF +NG RC+ R C+G DC D SDE C K C +F+CRD CI RC+ + DC D SDE +C A +C Y++ KC + LC CDG NDCG DE++C P N P + P++ EDDC T C C N C K C + G E+ C+ +QC C+ + CD DC DG DE + T DE IP P C P EF C +G C CDG DC D SDE
Sbjct: 2517 TCNVHDEFECGNGDCIDFSRTCDGVVHCKDKSDEKQSYCSSRKCKKGFLHCMNG---------------------------RCVASRFWCNGVDDCGDNSDEVP--CNKTSCAAT-EFRCRDGS-CIGNSSRCNQFIDCEDASDEMNCTATDCSSYFKLGVKGTTFQKCEHTSLCYAPSWVCDGANDCGDYSDERNCPG-GRKPKCPANYFACPSGRCI-PMTWTCDKEDDCENGEDETHCSERQDKFCYPVQFECNNHRCISKLWVCDGADDC-----GDGSDEDSRCRLTTCSTGSFQCPGTYVCVPERWLCDGDKDCADGADETLAAGCLYNNTCDEREFMCGNRQCIPKHFVCDHDDDCGDGSDESPECEYPTCGP-HEFRCANGRCLSNSQWECDGEFDCHDHSDE 2888
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. SwissProt
Match: gi|1708867|sp|P98158.1|LRP2_RAT (RecName: Full=Low-density lipoprotein receptor-related protein 2; Short=LRP-2; AltName: Full=Glycoprotein 330; Short=gp330; AltName: Full=Megalin; Flags: Precursor) HSP 1 Score: 140.969 bits (354), Expect = 9.135e-34 Identity = 132/424 (31.13%), Postives = 185/424 (43.63%), Query Frame = 0
Query: 44 EAQCSRTDFICEDLACINFALRCDGKFDCEDRSDE---YQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPG--ECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVS----PISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSC---------SSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLD--EFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLG 447
+C ++ C + CI + +CD DC D SDE + +C P QF CNNGRCI Q +CD C D +DE D C CD T++ C + RCI + +C+G+ DC D SDE C V C F+C + CI R +CDG DC D SDE +C EC +F+C CI +R CD NDCG + DE+DC+ T +P VP ++ ++ E CPT P+ + C +N +++ SD+EI + + PCE+ PQ +++C + C+Y Q C+ DC DG DEK +E P P E+ C +G+C CD + DC D SDE C LG
Sbjct: 3593 HHRCESNEWQCANKRCIPQSWQCDSVNDCLDNSDEDTSHCASRTCRPG-QFKCNNGRCIPQSWKCDVDNDCGDYSDE------------------------PIDECTTAAYNCDNHTEFSCKTNYRCIPQWAVCNGFDDCRDNSDEQG--CESVPCHPSGDFRCAN-HHCIPLRWKCDGTDDCGDNSDEENCVPRECSESEFRC-ADQQCIPSRWVCDQENDCGDNSDERDCEMKTCHPEHFQCTSGHCVPKALACDGRADCLDASDESACPTRFPNGT--YCPAAMFECKNHVCIQSFWICDGENDCVDGSDEEIHLCFNIPCES------PQ-----RFRCDNSRCVYGHQLCNGVDDCGDGSDEK-------EEHCRKPTHKPCTDTEYKCSNGNCISQHYVCDNVNDCGDLSDETGCNLG 3967 HSP 2 Score: 129.798 bits (325), Expect = 5.330e-30 Identity = 136/432 (31.48%), Postives = 182/432 (42.13%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDEY--QCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF----------------DPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC---FAVECDYWQFKCRISGLCIDTRRRCDGTNDCG--SDEDEQ---DCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSM---SRPVCGTDGLTYEN-ECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPL 446
+C++ F C + CIN +CD DC D SDE C +T FTC NGRC+ RCD C D++DE GC + + F+ NGR I L++ C P C + +C C+ + LCDG DC D SDE+ C C +FQC QRCI CDG +DC D SDE D C QF+C +G CI CDG NDCG SDED++ + N + F N P P + DC + + C + N C ++ C + Y ++E C NQ+ C +G CI CD DC DG DE+ E + P P C PL +F C +G C + C+ + DC D SDE C +
Sbjct: 2699 TRCNQLQFTCLNGHCINQDWKCDNDNDCGDGSDELPTVCAFHTCRSTAFTCGNGRCVPYHYRCDYYNDCGDNSDEAGCLFRNCNSTTEFTCSNGRCIPLSYVCNGINNCHDNDTSDEKNCPPHTCPPDFT-KCQTTNICVPRAFLCDGDNDCGDGSDENPIYCASHTCRSN-EFQCLSPQRCIPSYWFCDGEADCADGSDEPDTCGHSVNTCRASQFQCD-NGRCISGNWVCDGDNDCGDMSDEDQRHHCELQNCSSTQFTCVN--SRPPNRRCIPQYWVCDGDADCSDALDELQNCTMRTCSAGEFSCANGRCVRQSFRCDRRNDCGDY------SDERGCSYPPCHANQFTCQNGRCIPRFFVCDEDNDCGDGSDEQ----EHLCHTPE-PTC-PLHQFRCDNGHCIEMGRVCNHVDDCSDNSDEKGCGI 3113 HSP 3 Score: 111.694 bits (278), Expect = 3.432e-24 Identity = 123/371 (33.15%), Postives = 150/371 (40.43%), Query Frame = 0
Query: 38 PQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQC---PPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGC--GAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFF-STNEIEEPVPTTVSPISIEVLPEDDC------PTFC-PSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEED-PVEPQGCAVNQWQCLDGS-CIYLKQQCDRKYDCRDGEDEK 393
P + P QC F C + C+ RCDG DC D SDE+QC +C P+ G+CI CD C D +DE C A S P T + C NH CI K +CD DC D SDE + C + QF+C D RCI CDG DC D SDE C + C QFKC CI++R RCDG DC + DE C T P +E + T P + E DC T C P P T L C KA C D + + + E+D P +P C QWQC S CI L CD +DC +G DE
Sbjct: 1016 PAREPPTQQCGSLSFPCNNGKCVPSFFRCDGVDDCHDNSDEHQCGVFNNTCSPSAFACVRGGQCIPGQWHCDRQNDCLDGSDEQNCPTHATSSTCP-------------------------STSFTCDNH-VCIPKDWVCDTDNDCSDGSDE--KNCQASGTCQPTQFRCPD-HRCISPLYVCDGDKDCADGSDEAGC-VLNCTSAQFKCADGSSCINSRYRCDGVYDCRDNSDEAGCP--TRPPGMCHPDEFQCQGDGTCIPNTWECDGHPDCIHGSDEHTGCVPKTCSP---THFLCDNGNCIYKAWICDGDNDCRDMSD-----EKDCPTQPFHCPSTQWQCPGYSTCINLSALCDGVFDCPNGTDES 1346 HSP 4 Score: 108.997 bits (271), Expect = 2.030e-23 Identity = 138/490 (28.16%), Postives = 186/490 (37.96%), Query Frame = 0
Query: 6 KRKIFIVEYFL--VFRGKMS-----AVATRALNSSEGLMPQD---DIPEAQCSRTDFICEDLACI-----NFALRCDGKFDCEDRSDEYQCP--PSCDPNTQ------FTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFDPCVPGE--CDEE-----------------------TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRP---VCGTDGLTYENECRLKAM-SCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGS-CRPIDTRCDGMVDCEDESDE 441
K +FI ++ L + R + S V R ++S + D CS+T D + NF C + + + D+ C P+ +P TQ F CNNG+C+ RCDG C D++DE CG + +P F+ + G C+PG+ CD + T + C NH CI K +CD DC D SDE + C + QF+C D RCI CDG DC D SDE C + C QFKC CI++R RCDG DC + DE CPT P M P C DG N C + E P+ C+ + C +G+CIY CD DCRD DEK C P P P ++ C S C + CDG+ DC + +DE
Sbjct: 922 KDNVFITDWRLGAIIRVRKSDGGDMTVIRRGISSVMHVKAYDADLQTGSNYCSQTTHANGDCSHFCFPVPNFQRVCGCPYGMKLQRDQMTCEGDPAREPPTQQCGSLSFPCNNGKCVPSFFRCDGVDDCHDNSDEHQCGVFNNTCSPSAFACVRG-------GQCIPGQWHCDRQNDCLDGSDEQNCPTHATSSTCPSTSFTCDNHV-CIPKDWVCDTDNDCSDGSDE--KNCQASGTCQPTQFRCPD-HRCISPLYVCDGDKDCADGSDEAGC-VLNCTSAQFKCADGSSCINSRYRCDGVYDCRDNSDEAG-----------------------------------CPTRPPGMCHPDEFQCQGDGTCIPNTWECDGHPDCIHGSD-----------EHTGCVPKTCSPTHFLCDNGNCIYKAWICDGDNDCRDMSDEKDC--------PTQPFHCPSTQWQCPGYSTCINLSALCDGVFDCPNGTDE 1345 HSP 5 Score: 106.686 bits (265), Expect = 1.427e-22 Identity = 123/396 (31.06%), Postives = 166/396 (41.92%), Query Frame = 0
Query: 62 FALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC-NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE--NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDE---------QDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCP 445
F C F D C P C +TQF C NN +CI +CDG C D +DE + P F L Q++C + C + LC+ DC D SDED +C C E ++QC +K RCI + +CD +DCLD SDE + C + C QFKC +G CI +CD NDCG DE +CDN TE FS +P + ++ C S+ G D + C C + G EE+ V P+ C+ ++++C D CI + CD++ DC D DE+ C ++T C P + F C G C P CDG DC D SDE CP
Sbjct: 3491 FTCACPDDFQTVQLRDRTLCMPMCS-STQFLCGNNEKCIPIWWKCDGQKDCSDGSDE------PDLCPHRFCRLG--------------------QFQCRD-GNCTSPQALCNARQDCADGSDEDRVLCEHHRC-ESNEWQCANK-RCIPQSWQCDSVNDCLDNSDEDTSHCASRTCRPGQFKCN-NGRCIPQSWKCDVDNDCGDYSDEPIDECTTAAYNCDNHTE---FSCKTNYRCIPQWAVCNGFDDCRDNSDEQGCESVPCHPSG-DFRCANHHCIPLRWKCDGTDDC-----GDNSDEENCV-PRECSESEFRCADQQCIPSRWVCDQENDCGDNSDERDCEMKT---------CHP-EHFQCTSGHCVPKALACDGRADCLDASDESACP 3835 HSP 6 Score: 105.145 bits (261), Expect = 3.353e-22 Identity = 123/415 (29.64%), Postives = 162/415 (39.04%), Query Frame = 0
Query: 33 SEGLMPQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQC---PPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMC--PKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECD-YWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDE 441
S+G D P C F C D C + C+ + DC D SDE + C+ N ++ C N RCI Q +CD C D++DE S+ C PG Q++C N+ RCI + CD DC D SDE + C C +F C+ RCI + C+G+ DC D SDE C +V C F+C + CI R +CDGT+DCG + DE++C VP S + P SR VC EN+C ++E E + C +QC G C+ CD + DC D DE C P C P F CK+ C CDG DC D SDE
Sbjct: 3541 SDGSDEPDLCPHRFCRLGQFQCRDGNCTSPQALCNARQDCADGSDEDRVLCEHHRCESN-EWQCANKRCIPQSWQCDSVNDCLDNSDEDTSHCASRT-------------------CRPG------QFKC-NNGRCIPQSWKCDVDNDCGDYSDEPIDECTTAAYNCDNHTEFSCKTNYRCIPQWAVCNGFDDCRDNSDEQGCESVPCHPSGDFRC-ANHHCIPLRWKCDGTDDCGDNSDEENC-----------------VPRECSESEFRCADQQCIP------SRWVCDQ-----ENDCGDN-------------------SDERDCEMKTCHPEHFQCTSGHCVPKALACDGRADCLDASDESACPT----RFPNGTYC-PAAMFECKNHVCIQSFWICDGENDCVDGSDE 3875 HSP 7 Score: 100.908 bits (250), Expect = 9.689e-21 Identity = 124/390 (31.79%), Postives = 159/390 (40.77%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP-PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQD--STDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVP-----------GECDEETQWECANH------------RRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE---NDCFAVECDYWQFKCRISG----LCIDTRRRCDGTNDCGSDEDE-QDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSM--SRPVCGTDGLTYEN-ECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQ-GCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIE 398
C T F C + C+ + RCD DC D SDE C +C+ T+FTC+NGRCI C+G C D ++DE C P F+ T + CVP G+ +E CA+H +RCI CDG DC D SDE V QFQC D RCI CDG +DC D SDE + C C QF C S CI CDG DC DE Q+C T S E V S +DC + S P C + T +N C + C D + +G E E P+ C ++Q++C +G CI + + C+ DC D DEK C I
Sbjct: 2742 CRSTAFTCGNGRCVPYHYRCDYYNDCGDNSDEAGCLFRNCNSTTEFTCSNGRCIPLSYVCNGINNCHDNDTSDEKNCPP--HTCPPDFTKCQ------TTNICVPRAFLCDGDNDCGDGSDENPIYCASHTCRSNEFQCLSPQRCIPSYWFCDGEADCADGSDEPDTCGHSVNTCRASQFQC-DNGRCISGNWVCDGDNDCGDMSDEDQRHHCELQNCSSTQFTCVNSRPPNRRCIPQYWVCDGDADCSDALDELQNCTMRT----CSAGEFSCANGRCVRQ-SFRCDRRNDCGDYSDERGCSYPPCHANQFTCQNGRCIPRFFVCDEDNDCG---DGSDEQEHLCHTPEPTCPLHQFRCDNGHCIEMGRVCNHVDDCSDNSDEKGCGIN 3114 HSP 8 Score: 91.6633 bits (226), Expect = 7.560e-18 Identity = 105/362 (29.01%), Postives = 133/362 (36.74%), Query Frame = 0
Query: 90 QFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCE--TEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGST 449
F C+NG CI RCDGT C D TDEIGC P E + C CI +CD DC D +DE + C C + Q C + Q CI RCD SDC D SDE +C CD Q C +G C +T +RCD DC DE +C + F CG+ EC L+A C D + CE ++E C +Q+ C +G CI CD DC+D DE C + + C P + G C ID CDG+ DC + DE + G T
Sbjct: 32 NFRCDNGYCIPASWRCDGTRDCLDDTDEIGC---------------------------PPRSCESGLFLCPAEGTCIPSSWVCDEDKDCSDGADEQ-QNCAGTTCSAQ-QMTCSNGQ-CIPSEYRCDHVSDCPDGSDERNCHYPTCD--QLTC-ANGACYNTSQRCDQKVDCRDSSDEANCTTLCSQKEFE------------------------------------CGS------GECILRAYVCDHDND--------CEDNSDERNCNYDTCGGHQFTCSNGQCINQNWVCDGDDDCQDSGDEDGC-----ESNQSHHRCYPREWACPGSGRCISIDKVCDGVPDCPEGDDENNVTSGRT 305 HSP 9 Score: 83.9593 bits (206), Expect = 1.562e-15 Identity = 78/242 (32.23%), Postives = 103/242 (42.56%), Query Frame = 0
Query: 33 SEGLMPQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP-PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDY----WQFKCRISGLCIDTRRRCDGTNDCGSDEDEQD 269
S+G Q + CS C + CI RCD DC D SDE C P+CD Q TC NG C RCD C+DS+DE C + + ++EC + CI + +CD DC D SDE C CG QF C + Q CI++ CDG DC D DE+ C + + + ++ C SG CI + CDG DC +DE +
Sbjct: 94 SDGADEQQNCAGTTCSAQQMTCSNGQCIPSEYRCDHVSDCPDGSDERNCHYPTCD---QLTCANGACYNTSQRCDQKVDCRDSSDEANCTTLC----------------------------SQKEFECGS-GECILRAYVCDHDNDCEDNSDE--RNCNYDTCGGH-QFTCSNGQ-CINQNWVCDGDDDCQDSGDEDGCESNQSHHRCYPREWACPGSGRCISIDKVCDGVPDCPEGDDENN 299 HSP 10 Score: 77.0258 bits (188), Expect = 2.468e-13 Identity = 79/253 (31.23%), Postives = 107/253 (42.29%), Query Frame = 0
Query: 42 IPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPP-SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGE--CDEET-----------------QWECANHRRCIDKRRLCDGYPDCPDQSDED--AEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDN 272
+ +C +F C++ CI + RCDG DC D +DE CPP SC+ G CI CD C D DE A + + + NG+ C+P E CD + Q CAN C + + CD DC D SDE +C + E F+C + CI + CD +DC D SDE +C C QF C +G CI+ CDG +DC DE C++
Sbjct: 23 VSSQECGSGNFRCDNGYCIPASWRCDGTRDCLDDTDEIGCPPRSCESGLFLCPAEGTCIPSSWVCDEDKDCSDGADEQQNCAGTTCSAQQMTCSNGQ--------CIPSEYRCDHVSDCPDGSDERNCHYPTCDQLTCAN-GACYNTSQRCDQKVDCRDSSDEANCTTLCSQKE------FECGSGE-CILRAYVCDHDNDCEDNSDERNCNYDTCGGHQFTCS-NGQCINQNWVCDGDDDCQDSGDEDGCES 258 HSP 11 Score: 71.2478 bits (173), Expect = 1.607e-11 Identity = 82/261 (31.42%), Postives = 102/261 (39.08%), Query Frame = 0
Query: 190 PKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC--FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENE-CRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQG-CAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGS-CRPIDTRCDGMVDCEDESDEFDCP 445
P +CG F C + +C+ RCDG DC D SDE+ C F C F C G CI + CD NDC DEQ+C P + CPS S T +N C K C +D + +G ++E + G C Q++C D CI CD DC DG DE C + NC +F C DGS C RCDG+ DC D SDE CP
Sbjct: 1021 PTQQCGS-LSFPCNNG-KCVPSFFRCDGVDDCHDNSDEHQCGVFNNTCSPSAFACVRGGQCIPGQWHCDRQNDCLDGSDEQNC------------------------------PTHATSSTCPSTS--------FTCDNHVCIPKDWVCDTDNDCS---DG---SDEKNCQASGTCQPTQFRCPDHRCISPLYVCDGDKDCADGSDEAGCVL----------NCTSA-QFKCADGSSCINSRYRCDGVYDCRDNSDEAGCP 1224 HSP 12 Score: 67.0106 bits (162), Expect = 3.118e-10 Identity = 78/255 (30.59%), Postives = 104/255 (40.78%), Query Frame = 0
Query: 193 ECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDE-QDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYEN-ECRLKAMSCSSDQEIQIYYEGPCETEEDPVE-PQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
ECG F+C D CI RCDG DCLD++DE C C+ F C G CI + CD DC DE Q+C T + T + +P+ + P+ C P C D LT N C + C DQ++ C D C+ +++C G CI CD DC D DE+ C+ +T +FTC +G C + CDG DC+D DE C
Sbjct: 27 ECGSG-NFRC-DNGYCIPASWRCDGTRDCLDDTDEIGCPPRSCESGLFLCPAEGTCIPSSWVCDEDKDCSDGADEQQNCAGTTCSAQQMTCSNGQCIPSEYRCDHVSDCPDGSDERNC---HYPTC--DQLTCANGACYNTSQRC--DQKVD------CRDSSDEANCTTLCSQKEFECGSGECILRAYVCDHDNDCEDNSDERNCNYDTCGG----------HQFTCSNGQCINQNWVCDGDDDCQDSGDEDGC 256
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. SwissProt
Match: gi|160409939|sp|A2ARV4.1|LRP2_MOUSE (RecName: Full=Low-density lipoprotein receptor-related protein 2; Short=LRP-2; AltName: Full=Glycoprotein 330; Short=gp330; AltName: Full=Megalin; Flags: Precursor) HSP 1 Score: 139.043 bits (349), Expect = 4.722e-33 Identity = 133/423 (31.44%), Postives = 184/423 (43.50%), Query Frame = 0
Query: 44 EAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNT----QFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVS----PISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSC---------SSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPL--DEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLG 447
+C ++ C + CI +CD DC D SDE P C T QF CNNGRCI Q +CD C D +DE I + ++ CD T++ C + RCI + +C+G+ DC D SDE C V C F+C + CI R +CDG DC D SDE C EC +F+C CI +R CD NDCG + DE+DC+ T +P VP ++ ++ E CPT P+ + C +N +++ SD+EI + + PCE+ PQ +++C + CIY Q C+ DC DG DEK +E P P E+ C +G+C CD + DC D SDE C LG
Sbjct: 3593 HHRCEANEWQCANKRCIPEYWQCDSVDDCLDNSDED--PSHCASRTCRPGQFKCNNGRCIPQSWKCDVDNDCGDYSDE----PIHECMTAAYN------------------CDNHTEFSCKTNYRCIPQWAVCNGFDDCRDNSDEQG--CESVPCHPSGDFRCGN-HHCIPLRWKCDGIDDCGDNSDEESCVPRECTESEFRC-ADQQCIPSRWVCDQENDCGDNSDERDCEMKTCHPEHFQCTSGHCVPKALACDGRADCLDASDESACPTRFPNGT--YCPAAMFECKNHVCIQSFWICDGENDCVDGSDEEIHLCFNVPCES------PQ-----RFRCDNSRCIYGHQLCNGVDDCGDGSDEK-------EEHCRKPTHKPCTDTEYKCSNGNCVSQHYVCDNVDDCGDLSDETGCNLG 3967 HSP 2 Score: 130.954 bits (328), Expect = 2.089e-30 Identity = 139/441 (31.52%), Postives = 190/441 (43.08%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDEY--QCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF----------------DPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC---FAVECDYWQFKCRISGLCIDTRRRCDGTNDCG--SDEDEQ---DCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKA-----MSCSSDQEIQIYYEGPCETEE---DPVEPQGCAV-----NQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPL 446
A+C++ F C + CI+ +CD DC D SDE C +T FTC NGRC+ RCD C D++DE GC S + F+ NGR I L++ C P C + +C C+ + LCDG DC D SDE+ C C +FQC RCI CDG +DC+D SDE D C QF C +G CI + CDG NDCG SDED++ + N + F N P P + DC D L C ++A SC++ + I+ + C+ D + +GC+ +Q+ C +G CI CD DC DG DE+ E + P P C P +F C +G C + T C+ + DC D SDE C +
Sbjct: 2699 ARCNQFQFTCLNGRCISQDWKCDNDNDCGDGSDELPTVCAFHTCRSTAFTCANGRCVPYHYRCDFYNDCGDNSDEAGCLFRSCNSTTEFTCSNGRCIPLSYVCNGINNCHDNDTSDEKNCPPITCQPDFA-KCQTTNICVPRAFLCDGDNDCGDGSDENPIYCASHTCRSN-EFQCVSPHRCIPSYWFCDGEADCVDSSDEPDTCGHSLNSCSANQFHCD-NGRCISSSWVCDGDNDCGDMSDEDQRHHCELQNCSSTEFTCIN--SRPPNRRCIPQHWVCDGDADC-------------ADALDELQNCTMRACSTGEFSCANGRCIRQSFR--CDRRNDCGDYSDERGCSYPPCRDDQFTCQNGQCITKLYVCDEDNDCGDGSDEQ----EHLCHTPE-PTCPP-HQFRCDNGHCIEMGTVCNHVDDCSDNSDEKGCGI 3113 HSP 3 Score: 115.931 bits (289), Expect = 1.471e-25 Identity = 137/469 (29.21%), Postives = 184/469 (39.23%), Query Frame = 0
Query: 16 LVFRGKMSAVATRA----LNSSEGLMPQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP--PSCDPNTQ------FTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFDPCVPGE--CDEE-----------------------TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRP---VCGTDGLTYENECRLKAMSCSSDQE-IQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGS-CRPIDTRCDGMVDCEDESDE 441
+V RG S + +A L + Q P CS F NF C + + + D+ C P+ +P TQ F CNNG+C+ RCDG C D++DE CGA++ + F+ ++G C+PG+ CD++ T + C NH CI K +CD DC D SDE + C QF+C D RCI CDG DC+D SDE C + C QFKC CI++R RCDG DC + DE CPT P M P C DG C C + IQ E + P+ C+ + + C +G+CIY CD DCRD DEK C P P P ++ C S C + CDG+ DC + +DE
Sbjct: 948 VVRRGISSIMHVKAYDADLQTGTNYCSQTTHPNGDCSHFCF-----PVPNFQRVCGCPYGMKLQRDQMTCEGDPAREPPTQQCGSSSFPCNNGKCVPSIFRCDGVDDCHDNSDEHQCGALNNTCSSSAFTCVHG-------GQCIPGQWRCDKQNDCLDGSDEQNCPTRSPSSTCPPTSFTCDNH-MCIPKEWVCDTDNDCSDGSDE--KNCQASGTCHPTQFRCPD-HRCISPLYVCDGDKDCVDGSDEAGC-VLNCTSSQFKCADGSSCINSRYRCDGVYDCKDNSDEA-----------------------------------GCPTRPPGMCHPDEFQCQGDG-----TCIPNTWECDGHPDCIQ------GSDEHNGCVPKTCSPSHFLCDNGNCIYNSWVCDGDNDCRDMSDEKDC--------PTQPFHCPSSQWQCPGYSICVNLSALCDGVFDCPNGTDE 1345 HSP 4 Score: 105.916 bits (263), Expect = 1.925e-22 Identity = 122/404 (30.20%), Postives = 168/404 (41.58%), Query Frame = 0
Query: 62 FALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC-NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE--NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDE---------QDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCE--------TEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCP 445
F C F D C P C +TQF C NN +CI +CDG C D +DE S + P F L Q++C + C + LC+ DC D SDED +C C E ++QC +K RCI + +CD DCLD SDE + C + C QFKC +G CI +CD NDCG DE +CDN TE FS +P + ++ C S+ C G C + I + ++ C+ ++E+ P+ C ++++C D CI + CD++ DC D DE+ C ++T C P + F C G C P CDG DC D SDE CP
Sbjct: 3491 FTCECPDDFQTVQLRDRTLCMPMCS-STQFLCGNNEKCIPIWWKCDGQKDCSDGSDE------SDLCPHRFCRLG--------------------QFQCRD-GNCTSPQALCNARQDCADGSDEDRVLCEHHRC-EANEWQCANK-RCIPEYWQCDSVDDCLDNSDEDPSHCASRTCRPGQFKCN-NGRCIPQSWKCDVDNDCGDYSDEPIHECMTAAYNCDNHTE---FSCKTNYRCIPQWAVCNGFDDCRDNSDEQGCESVP---CHPSG----------DFRCGNHHCIPLRWK--CDGIDDCGDNSDEESCVPRECTESEFRCADQQCIPSRWVCDQENDCGDNSDERDCEMKT---------CHP-EHFQCTSGHCVPKALACDGRADCLDASDESACP 3835 HSP 5 Score: 102.449 bits (254), Expect = 2.445e-21 Identity = 124/418 (29.67%), Postives = 162/418 (38.76%), Query Frame = 0
Query: 46 QCSRTDFIC-EDLACINFALRCDGKFDCEDRSDEYQ-CPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDE--IGCGAISKVAPGFFSSLNGRIIGL-----TFDPCV------PGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAE--MCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECD-YWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDE 441
CS T F+C + CI +CDG+ DC D SDE CP QF C +G C C+ C D +DE + C + + N R I + D C+ P C T Q++C N+ RCI + CD DC D SDE M C +F C+ RCI + C+G+ DC D SDE C +V C F+C + CI R +CDG +DCG + DE+ C VP + + P SR VC EN+C ++E E + C +QC G C+ CD + DC D DE C P C P F CK+ C CDG DC D SDE
Sbjct: 3513 MCSSTQFLCGNNEKCIPIWWKCDGQKDCSDGSDESDLCPHRFCRLGQFQCRDGNCTSPQALCNARQDCADGSDEDRVLCEH-HRCEANEWQCANKRCIPEYWQCDSVDDCLDNSDEDPSHCASRTCRPGQFKC-NNGRCIPQSWKCDVDNDCGDYSDEPIHECMTAAYNCDNHTEFSCKTNYRCIPQWAVCNGFDDCRDNSDEQGCESVPCHPSGDFRCG-NHHCIPLRWKCDGIDDCGDNSDEESC-----------------VPRECTESEFRCADQQCIP------SRWVCDQ-----ENDCGDN-------------------SDERDCEMKTCHPEHFQCTSGHCVPKALACDGRADCLDASDESACPT----RFPNGTYC-PAAMFECKNHVCIQSFWICDGENDCVDGSDE 3875 HSP 6 Score: 97.4413 bits (241), Expect = 9.186e-20 Identity = 81/237 (34.18%), Postives = 109/237 (45.99%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQC-PPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE-----CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITEN 276
+C+ ++F C D CI CD + DC D SDE C +C P F C +G C+ + L CDG C D++DE C R T+ P +EC NH CI +CDG DC D SDE+ +C V C +F+C D RCI + C+G DC D SDE + + C ++KC +G C+ CD +DCG DE C N+ EN
Sbjct: 3760 ECTESEFRCADQQCIPSRWVCDQENDCGDNSDERDCEMKTCHPE-HFQCTSGHCVPKALACDGRADCLDASDESACPT--------------RFPNGTYCP--------AAMFECKNH-VCIQSFWICDGENDCVDGSDEEIHLCFNVPCESPQRFRC-DNSRCIYGHQLCNGVDDCGDGSDEKEEHCRKPTHKPCTDTEYKCS-NGNCVSQHYVCDNVDDCGDLSDETGC-NLGEN 3969 HSP 7 Score: 93.2041 bits (230), Expect = 2.316e-18 Identity = 105/361 (29.09%), Postives = 134/361 (37.12%), Query Frame = 0
Query: 90 QFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCE--TEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTC-KDGSCRPIDTRCDGMVDCEDESDEFDCPLG 447
F C+NG CI RCDGT C D TDEIGC S GFF C CI +CD DC D +DE + CP C + Q C + Q C+ RCD SDC D SDE +C+ CD Q C +G C +T ++CD DC DE +C + F CG+ EC L+A C D + CE ++E C +Q+ C +G CI CD DC+D DE C P E+ C G C +D CDG+ DC + DE + G
Sbjct: 32 NFRCDNGYCIPASWRCDGTRDCLDDTDEIGCPPRS-CGSGFFL--------------------------CPAEGTCIPSSWVCDQDKDCSDGADEQ-QNCPGTTCSSQ-QLTCSNGQ-CVPIEYRCDHVSDCPDGSDERNCYYPTCD--QLTC-ANGACYNTSQKCDHKVDCRDSSDEANCTTLCSQKEFQ------------------------------------CGS------GECILRAYVCDHDND--------CEDNSDEHNCNYDTCGGHQFTCSNGQCINQNWVCDGDDDCQDSGDEDGCESNQRHHTCYP------REWACPGSGRCISMDKVCDGVPDCPEGEDENNATSG 303 HSP 8 Score: 87.0409 bits (214), Expect = 1.975e-16 Identity = 78/242 (32.23%), Postives = 104/242 (42.98%), Query Frame = 0
Query: 33 SEGLMPQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQC-PPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE----CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQD 269
S+G Q + P CS C + C+ RCD DC D SDE C P+CD Q TC NG C +CD C+DS+DE C + + +++C + CI + +CD DC D SDE C CG QF C + Q CI++ CDG DC D DE+ C + + C ++ C SG CI + CDG DC EDE +
Sbjct: 94 SDGADEQQNCPGTTCSSQQLTCSNGQCVPIEYRCDHVSDCPDGSDERNCYYPTCD---QLTCANGACYNTSQKCDHKVDCRDSSDEANCTTLC----------------------------SQKEFQCGS-GECILRAYVCDHDNDCEDNSDE--HNCNYDTCGGH-QFTCSNGQ-CINQNWVCDGDDDCQDSGDEDGCESNQRHHTCYPREWACPGSGRCISMDKVCDGVPDCPEGEDENN 299 HSP 9 Score: 77.0258 bits (188), Expect = 2.599e-13 Identity = 80/253 (31.62%), Postives = 110/253 (43.48%), Query Frame = 0
Query: 42 IPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC-NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGE--CDEET-----------------QWECANHRRCIDKRRLCDGYPDCPDQSDED--AEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDN 272
+ +C +F C++ CI + RCDG DC D +DE CPP + F C G CI CD C D DE + PG ++ + + + + CVP E CD + Q CAN C + + CD DC D SDE +C + E FQC + CI + CD +DC D SDE++C C QF C +G CI+ CDG +DC DE C++
Sbjct: 23 VSGQECGSGNFRCDNGYCIPASWRCDGTRDCLDDTDEIGCPPRSCGSGFFLCPAEGTCIPSSWVCDQDKDCSDGADE------QQNCPG--TTCSSQQLTCSNGQCVPIEYRCDHVSDCPDGSDERNCYYPTCDQLTCAN-GACYNTSQKCDHKVDCRDSSDEANCTTLCSQKE------FQCGSGE-CILRAYVCDHDNDCEDNSDEHNCNYDTCGGHQFTCS-NGQCINQNWVCDGDDDCQDSGDEDGCES 258 HSP 10 Score: 71.633 bits (174), Expect = 1.231e-11 Identity = 91/305 (29.84%), Postives = 123/305 (40.33%), Query Frame = 0
Query: 190 PKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPED---DCPTFCPSMS-RPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQ---GCAVN----QWQCLDGS-CIYLKQQCDRKYDCRDGEDEKYCS---------------------------------IETIDEIP--APPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCP 445
P +CG F C + +C+ RCDG DC D SDE+ C A+ C F C G CI + RCD NDC DEQ+C T +P + PT+ + + +P++ D C S C G + + R C S + C+ ++D V+ GC +N Q++C DGS CI + +CD YDC+D DE C I+ DE P C P F C +G+C CDG DC D SDE DCP
Sbjct: 1021 PTQQCGSS-SFPCNNG-KCVPSIFRCDGVDDCHDNSDEHQCGALNNTCSSSAFTCVHGGQCIPGQWRCDKQNDCLDGSDEQNCP--TRSPSSTC------PPTSFTCDNHMCIPKEWVCDTDNDCSDGSDEKNCQASGTCHPTQFRCPDHRCISPLYV-------CDGDKDCVDGSDEAGCVLNCTSSQFKCADGSSCINSRYRCDGVYDCKDNSDEAGCPTRPPGMCHPDEFQCQGDGTCIPNTWECDGHPDCIQGSDEHNGCVPKTCSP-SHFLCDNGNCIYNSWVCDGDNDCRDMSDEKDCP 1307 HSP 11 Score: 65.0846 bits (157), Expect = 1.186e-9 Identity = 74/256 (28.91%), Postives = 91/256 (35.55%), Query Frame = 0
Query: 193 ECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYE----GPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
ECG F+C D CI RCDG DCLD++DE C C F C G CI + CD DC DEQ +CP GT C + ++CS+ Q + I Y C D +Q C +G+C Q+CD K DCRD DE C+ + EF C G C CD DCED SDE +C
Sbjct: 27 ECGSG-NFRC-DNGYCIPASWRCDGTRDCLDDTDEIGCPPRSCGSGFFLCPAEGTCIPSSWVCDQDKDCSDGADEQ----------------------------------QNCP-----------GT-------TCSSQQLTCSNGQCVPIEYRCDHVSDCPDGSDERNCYYPTCDQLTCANGACYNTSQKCDHKVDCRDSSDEANCTTLCSQK-----------EFQCGSGECILRAYVCDHDNDCEDNSDEHNC 217
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. SwissProt
Match: gi|160332309|sp|P98164.3|LRP2_HUMAN (RecName: Full=Low-density lipoprotein receptor-related protein 2; Short=LRP-2; AltName: Full=Glycoprotein 330; Short=gp330; AltName: Full=Megalin; Flags: Precursor) HSP 1 Score: 133.65 bits (335), Expect = 2.721e-31 Identity = 129/420 (30.71%), Postives = 183/420 (43.57%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDE---YQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGE--CDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFF----STNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAV---------NQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLG 447
C ++ C + CI + +CD DCED SDE + +C P QF C NGRCI Q +CD C D +DE + C+ CD T++ C + RCI K +C+G DC D SDE C + C F+C++ CI R +CDG +DC D SDE +C EC +F+C ++ CI +R CD NDCG + DE+DC+ T +P + S + + + S ++ E DCPT P DG C+ C + I Y++ + + + + N+++C + CIY + C+ DC DG DE E P P C E+ C +G C P D CD DC D SDE C G
Sbjct: 3593 HCDSNEWQCANKRCIPESWQCDTFNDCEDNSDEDSSHCASRTCRPG-QFRCANGRCIPQAWKCDVDNDCGDHSDE------------------------PIEECMSSAHLCDNFTEFSCKTNYRCIPKWAVCNGVDDCRDNSDEQG--CEERTCHPVGDFRCKN-HHCIPLRWQCDGQNDCGDNSDEENCAPRECTESEFRC-VNQQCIPSRWICDHYNDCGDNSDERDCEMRTCHPEYFQCTSGHCVHSELKCDGSADCLDASDEADCPTRFP---------DGAY----CQATMFECKNHVCIPPYWKCDGDDDCGDGSDEELHLCLDVPCNSPNRFRCDNNRCIYSHEVCNGVDDCGDGTDET----EEHCRKPTPKPCTEY-EYKCGNGHCIPHDNVCDDADDCGDWSDELGCNKG 3965 HSP 2 Score: 121.324 bits (303), Expect = 2.437e-27 Identity = 142/438 (32.42%), Postives = 181/438 (41.32%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQ---CPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDEETQ----------WECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE-NDCFAVE--CDYWQFKCRISGLCIDTRRRCDGTNDCG--SDEDEQD---CDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFC---PSMSRPVCGTDGLT-YENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPS 453
+C + F C + CI+ +CD DC D SDE + +C P T FTC NGRC+ RCD C D +DE GC A F N R I F D C +E +C N CI + LCDG DC D SDE+ C C +FQC RCI + CD +DC D SDE C E C +FKC G CI + CDG NDCG SDED++ N +++ F N + P P S + DC + +R C + T C K C + Y ++E Q C NQ+ C +G CI CD DC DG DE T + P C P EF C +G C + C+ + DC D SDE C + PS
Sbjct: 2699 RCGASSFTCSNGRCISEEWKCDNDNDCGDGSDEMESVCALHTCSP-TAFTCANGRCVQYSYRCDYYNDCGDGSDEAGCLFRDCNATTEFMCNNRRCIPREFICNGVDNCHDNNTSDEKNCPDRTCQSGYTKCHNSNICIPRVYLCDGDNDCGDNSDENPTYCTTHTCSSS-EFQCA-SGRCIPQHWYCDQETDCFDASDEPASCGHSERTCLADEFKCD-GGRCIPSEWICDGDNDCGDMSDEDKRHQCQNQNCSDSEFLCVN--DRPPDRRCIPQSWVCDGDVDCTDGYDENQNCTRRTCSENEFTCGYGLCIPKIFRCDRHNDCGDY------SDERGCLYQTCQQNQFTCQNGRCISKTFVCDEDNDCGDGSDELMHLCHTPE-----PTCPP-HEFKCDNGRCIEMMKLCNHLDDCLDNSDEKGCGINECHDPS 3118 HSP 3 Score: 115.546 bits (288), Expect = 2.099e-25 Identity = 93/243 (38.27%), Postives = 118/243 (48.56%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP---PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDE--------ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECG--EEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE-NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
CS + F C CI RCD + DC D SDE+ CP P+ +TQ+TC+N +CI ++ CD C D +DE C + P F+ N R I L+F CV G DE +Q++CA+ +CI CDG DC D SDE CP G +FQC++ CI CDG+ DCL SDE N C C F C +G CI CD NDCG DE+DC
Sbjct: 1067 CSSSAFTCGHGECIPAHWRCDKRNDCVDGSDEHNCPTHAPASCLDTQYTCDNHQCISKNWVCDTDNDCGDGSDEKNCNSTETCQPSQFNCPNHRCIDLSFVCDGDKDCVDGS-DEVGCVLNCTASQFKCASGDKCIGVTNRCDGVFDCSDNSDEAG--CPTRPPGMCHSDEFQCQEDGICIPNFWECDGHPDCLYGSDEHNACVPKTCPSSYFHCD-NGNCIHRAWLCDRDNDCGDMSDEKDC 1305 HSP 4 Score: 114.005 bits (284), Expect = 5.932e-25 Identity = 121/372 (32.53%), Postives = 153/372 (41.13%), Query Frame = 0
Query: 38 PQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCD--PNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFF-STNEIEEPVPTTVSPISIEVLPEDDC-----------PTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEED-PVEPQGCAVNQWQCLDGS-CIYLKQQCDRKYDCRDGEDEK 393
P ++ P QC F C++ C+ CDG DC D SDE C + ++ FTC +G CI RCD C D +DE C + P C +TQ+ C NH +CI K +CD DC D SDE + C E + QF C + RCID CDG DC+D SDE C + C QFKC CI RCDG DC + DE C T P ++E + P E DC P CPS S C C +A C D + G E+D P +P C QWQCL + C+ L CD +DC +G DE
Sbjct: 1017 PTNEPPTEQCGLFSFPCKNGRCVPNYYLCDGVDDCHDNSDEQLCGTLNNTCSSSAFTCGHGECIPAHWRCDKRNDCVDGSDEHNCPTHA-----------------------PASC-LDTQYTCDNH-QCISKNWVCDTDNDCGDGSDE--KNCNSTETCQPSQFNCPN-HRCIDLSFVCDGDKDCVDGSDEVGC-VLNCTASQFKCASGDKCIGVTNRCDGVFDCSDNSDEAGCP--TRPPGMCHSDEFQCQEDGICIPNFWECDGHPDCLYGSDEHNACVPKTCPS-SYFHCD------NGNCIHRAWLCDRDNDC-----GDMSDEKDCPTQPFRCPSWQWQCLGHNICVNLSVVCDGIFDCPNGTDES 1345 HSP 5 Score: 108.227 bits (269), Expect = 3.972e-23 Identity = 118/398 (29.65%), Postives = 167/398 (41.96%), Query Frame = 0
Query: 62 FALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC-NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVEC-GEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE--NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDE---------QDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMS-RPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCP 445
F C F S C P C +TQF C NN +CI +CDG C D +DE+ + P F L Q++C++ C + LC+ + +CPD SDED +C C EW QC +K RCI + +CD ++DC D SDE + C + C QF+C +G CI +CD NDCG DE CDN TE FS +P ++ ++ C + PV D + C C + ++E+ P+ C ++++C++ CI + CD DC D DE+ C + T C P + F C G C + +CDG DC D SDE DCP
Sbjct: 3489 FTCECPDDFRTLQLSGSTYCMPMCS-STQFLCANNEKCIPIWWKCDGQKDCSDGSDELA------LCPQRFCRLG--------------------QFQCSD-GNCTSPQTLCNAHQNCPDGSDEDRLLCENHHCDSNEW--QCANK-RCIPESWQCDTFNDCEDNSDEDSSHCASRTCRPGQFRC-ANGRCIPQAWKCDVDNDCGDHSDEPIEECMSSAHLCDNFTE---FSCKTNYRCIPKWAVCNGVDDCRDNSDEQGCEERTCHPV--GDFRCKNHHCIPLRWQCDGQNDCGD------NSDEENCAPRECTESEFRCVNQQCIPSRWICDHYNDCGDNSDERDCEMRT---------CHP-EYFQCTSGHCVHSELKCDGSADCLDASDEADCP 3833 HSP 6 Score: 85.8853 bits (211), Expect = 4.305e-16 Identity = 80/237 (33.76%), Postives = 104/237 (43.88%), Query Frame = 0
Query: 39 QDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP-PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFA-----VECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQD 269
+ D ++ CS C + CI RCD DC D +DE C P+C+ Q TC+NG C +CD C+DS+DEI C I C+ E + C N CI + +CD DC D SDE A C CG +QF C RCI + CDG DC D DE+ C + +C ++ C SG CI + CDG DC EDE +
Sbjct: 100 RQDCSQSTCSSHQITCSNGQCIPSEYRCDHVRDCPDGADENDCQYPTCE---QLTCDNGACYNTSQKCDWKVDCRDSSDEINCTEI----------------------CLHNE------FSCGN-GECIPRAYVCDHDNDCQDGSDEHA--CNYPTCG-GYQFTC-PSGRCIYQNWVCDGEDDCKDNGDEDGCESGPHDVHKCSPREWSCPESGRCISIYKVCDGILDCPGREDENN 300 HSP 7 Score: 83.5741 bits (205), Expect = 2.114e-15 Identity = 106/361 (29.36%), Postives = 139/361 (38.50%), Query Frame = 0
Query: 88 NTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTC-KDGSCRPIDTRCDGMVDCEDESDEFDCPLG 447
+ F C +G CI D RCDGT C D DEIGC A+ G+F +C + +CI +CD DC D SDE + C + C Q C + Q CI RCD DC D +DENDC C+ Q C +G C +T ++CD DC DE +C I + FS CG EC +A C D + Q +G ++E C Q+ C G CIY CD + DC+D DE C D P E++C + G C I CDG++DC DE + G
Sbjct: 30 SAHFRCGSGHCIPADWRCDGTKDCSDDADEIGC-AVVTCQQGYF--------------------------KCQSEGQCIPNSWVCDQDQDCDDGSDERQD-CSQSTCSSH-QITCSNGQ-CIPSEYRCDHVRDCPDGADENDCQYPTCE--QLTCD-NGACYNTSQKCDWKVDCRDSSDEINCTEICLHNEFS------------------------------------CG------NGECIPRAYVCDHDNDCQ---DG---SDEHACNYPTCGGYQFTCPSGRCIYQNWVCDGEDDCKDNGDEDGCESGPHDVHKCSPR-----EWSCPESGRCISIYKVCDGILDCPGREDENNTSTG 304 HSP 8 Score: 72.4034 bits (176), Expect = 6.525e-12 Identity = 65/199 (32.66%), Postives = 83/199 (41.71%), Query Frame = 0
Query: 52 FICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDA-EMCPK-VECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEND-----------CFAVECDY 237
C++ AC N + +CD K DC D SDE C C N +F+C NG CI + CD CQD +DE C P G Q+ C + RCI + +CDG DC D DED E P V ++ C + RCI + CDG DC DEN+ C A+ C Y
Sbjct: 150 LTCDNGACYNTSQKCDWKVDCRDSSDEINCTEICLHN-EFSCGNGECIPRAYVCDHDNDCQDGSDEHACNY----------------------PTCGG-----YQFTCPS-GRCIYQNWVCDGEDDCKDNGDEDGCESGPHDVHKCSPREWSCPESGRCISIYKVCDGILDCPGREDENNTSTGKYCSMTLCSALNCQY 319 HSP 9 Score: 68.1662 bits (165), Expect = 1.279e-10 Identity = 90/297 (30.30%), Postives = 116/297 (39.06%), Query Frame = 0
Query: 164 RCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC---FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEED----------PVEPQG-CAVNQWQCL-DGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCP 445
RC+ LCDG DC D SDE C F C + CI RCD +DC+D SDE++C C Q+ C + CI CD NDCG DE++C N E P+ + + + D C V G+D + C C+S + I C+ D P P G C +++QC DG CI +CD DC G DE + P C P F C +G+C CD DC D SDE DCP
Sbjct: 1037 RCVPNYYLCDGVDDCHDNSDEQLCGTLNNTCSSS-AFTCGHGE-CIPAHWRCDKRNDCVDGSDEHNCPTHAPASCLDTQYTCD-NHQCISKNWVCDTDNDCGDGSDEKNC-----------NSTETCQPSQFNCPNHRCI---DLSFVCDGDKDCVDGSDEVGCVLNCTASQFKCASGDKC-IGVTNRCDGVFDCSDNSDEAGCPTRPPGMCHSDEFQCQEDGICIPNFWECDGHPDCLYGSDEHNACV--------PKTC-PSSYFHCDNGNCIHRAWLCDRDNDCGDMSDEKDCP 1306 HSP 10 Score: 67.781 bits (164), Expect = 2.015e-10 Identity = 72/216 (33.33%), Postives = 92/216 (42.59%), Query Frame = 0
Query: 44 EAQCSRTDFICEDLACINFALRCDGKFDCEDRSDE---YQCPPSCDPNTQFTCNNG-----RCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF--------------DPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMC--PKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVEC 235
E C +F C+ CI CDG DC D SDE +QC +++F C N RCI Q CDG C D DE + F+ G I F C+ C ++ Q+ C N RCI K +CD DC D SDE +C P+ C +F+C D RCI+ + C+ DCLD SDE C EC
Sbjct: 2903 ERTCLADEFKCDGGRCIPSEWICDGDNDCGDMSDEDKRHQCQNQNCSDSEFLCVNDRPPDRRCIPQSWVCDGDVDCTDGYDENQNCTRRTCSENEFTCGYGLCIPKIFRCDRHNDCGDYSDERGCLYQTC-QQNQFTCQN-GRCISKTFVCDEDNDCGDGSDELMHLCHTPEPTCPPH-EFKC-DNGRCIEMMKLCNHLDDCLDNSDEKGCGINEC 3114 HSP 11 Score: 67.0106 bits (162), Expect = 2.987e-10 Identity = 77/260 (29.62%), Postives = 99/260 (38.08%), Query Frame = 0
Query: 190 PKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYEN-ECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDG-SCRPIDTRCDGMVDCEDESDEFDCP 445
P +CG + F C++ RC+ CDG DC D SDE C + C F C G CI RCD NDC DE +C PT P+ C T +N +C K C +D + G E++ + C +Q+ C + CI L CD DC DG DE C + NC +F C G C + RCDG+ DC D SDE CP
Sbjct: 1022 PTEQCGL-FSFPCKNG-RCVPNYYLCDGVDDCHDNSDEQLCGTLNNTCSSSAFTCG-HGECIPAHWRCDKRNDCVDGSDEHNC-----------------------------------PTHAPAS----CLDTQYTCDNHQCISKNWVCDTDNDC-----GDGSDEKNCNSTETCQPSQFNCPNHRCIDLSFVCDGDKDCVDGSDEVGCVL----------NCTA-SQFKCASGDKCIGVTNRCDGVFDCSDNSDEAGCP 1223
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. SwissProt
Match: gi|1270743847|sp|C0HL13.1|LRP2_PIG (RecName: Full=Low-density lipoprotein receptor-related protein 2; Short=LRP-2; AltName: Full=Glycoprotein 330; Short=gp330; AltName: Full=Megalin; Flags: Precursor) HSP 1 Score: 127.102 bits (318), Expect = 3.921e-29 Identity = 122/420 (29.05%), Postives = 184/420 (43.81%), Query Frame = 0
Query: 43 PEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDE--YQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFDPCVPGEC------------------DEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVS----PISIEVLPEDDCPT------FCPSM---------SRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSC 422
P+ C F C D C + C+ + DC D SDE C + Q+ C N RCI + +CD C D++DE + PG+F NG I ++ V +C D T+++C + RCI K +C+G+ DC D SDE + C + C +F+C + CI R RCDG++DC D SDE +C +C +F+C CI +R CD NDCG + DE+DC+ +T +P + +P + ++ E CPT +CP+ +P DG +N+C SD+E+ + C+ N+++C + CIY + C+ + DC DG DEK + P P C EF C +G C
Sbjct: 3547 PQRFCRLGQFQCSDGNCTSSNFICNARQDCPDGSDEDAVLCEHHRCESNQWQCANKRCIPESWQCDSLNDCGDNSDEDSSHCARRTCLPGYFKCANGHCIPQSWKCDVDNDCGDYSDEPLQECMGPAYRCDNYTEFDCKTNYRCIPKWAVCNGFDDCRDNSDE--QNCESLTCKPSGEFRCTN-HHCIPLRWRCDGHNDCGDNSDEENCVPRQCSESEFRCD-DQTCIPSRWICDQNNDCGDNSDERDCEVMTCHPGYFQCSSGHCIPDQMRCDGFADCLDASDEATCPTRFPNGAYCPATLFECKNHVCVQPSWKCDG---DNDC-----GDGSDEELHLCLNITCD-----------LTNRFRCDNNRCIYRHELCNHEDDCGDGSDEKKENCLA----PTPRPCTE-GEFKCSNGHC 3938 HSP 2 Score: 118.242 bits (295), Expect = 2.525e-26 Identity = 130/418 (31.10%), Postives = 168/418 (40.19%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQ--CPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDEETQ----------WECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE---CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFD 443
+C+ T F C CI +L+C+ +C D SDE + C P T FTC NGRCI + RCD C D++DE GC S FS NG+ + L D C +E +CAN CI + LCDG DC D SDE+ C C +FQC CI + CDG DC D SDE C +FKC + CI CDG NDCG DE D + EN S+ C P R C + C D + Y+ E + C+ +++C +G CI +CDR+ DC D DE+ C DE + FTC++G C CDG DC D SDE +
Sbjct: 2696 RCNSTQFTCLSGYCILESLKCNDIDECGDSSDELETLCAYHTCPPTSFTCANGRCIQRHFRCDHYNDCGDNSDESGCRFRSCNITTEFSCNNGKCLPLQLVCDGIDHCNDNNTSDEKNCAQHTCLPDYIKCANSNVCIPRLFLCDGDNDCGDMSDENPIYCVSPTCKNN-EFQCTSGS-CIPELWHCDGERDCDDGSDEPATCVYSPSTCSSDEFKCD-NNRCIQMEWICDGDNDCGDMSDE-DGRHHCENHNCSSYAFH-------------------CVNSAPPSRR-------------CIPLSWVCDGDADCSDAYD-----EHQNCTRRNCSGTEFRCSNGLCIPNWFRCDRRNDCGDYSDERNCKYPACDE----------NLFTCQNGICTYKSYICDGENDCGDNSDELE 3062 HSP 3 Score: 114.39 bits (285), Expect = 4.050e-25 Identity = 120/377 (31.83%), Postives = 149/377 (39.52%), Query Frame = 0
Query: 43 PEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPP---SCDPNTQFTCNN--GRCIGQDLRCDGTPQCQDSTDEIGCGAISKVA--PGFFSSLNGRII--------------GLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAV---ECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEED-PVEPQGCAVNQWQCLDGS-CIYLKQQCDRKYDCRDGEDEK 393
P QC F C + C+ RCDG DC D SDE QC SC P + F C + G CI RCD C D +DE C + ++ + +F+ N I G C GE TQ+ C NH RCID +CDG DC D SDE A + + C + QF+C +CI RCDG SDC D SDE DC C +F+CR G+CI CDG DC + DE CP SR +C C + C D + + E+D P +P C QWQC S C+ L CD DC G DE
Sbjct: 1022 PMEQCGALSFPCNNGRCVPLHYRCDGVDDCHDNSDEVQCGAFNTSCAP-SAFACGHGGGECIPSYWRCDNHNDCVDGSDEQNCSSQAQTSCRADYFTCDNHMCIPKNWLCDTDNDCGDGSDEKRCDLGETCSPTQFHCPNH-RCIDLAFVCDGDKDCADGSDESACV---INCTDS-QFKCVGSNKCISNTYRCDGVSDCSDHSDEIDCPTRPPGMCRQDEFQCREDGICIPDSWECDGHPDCLTGSDEH----------------------------------SGCPPRTCPXSRFLCA------NGNCIFRDWLCDGDNDCR-----DMSDEKDCPTQPFLCPSWQWQCPGHSICVNLSSVCDGISDCPHGTDES 1347 HSP 4 Score: 109.383 bits (272), Expect = 1.908e-23 Identity = 118/373 (31.64%), Postives = 153/373 (41.02%), Query Frame = 0
Query: 90 QFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAI-SKVAPGFFSSLNGRIIGL-------TFDPCVPG----ECDEETQ-------WECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEP-QGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGS-CRPIDTRCDGMVDCEDESDE 441
F CNNGRC+ RCDG C D++DE+ CGA + AP F+ +G + + CV G C + Q + C NH CI K LCD DC D SDE + C E QF C + RCID CDG DC D SDE+ C + C QFKC S CI RCDG +DC DE DCPT P M R E +CR + E + + ++E P + C +++ C +G+CI+ CD DCRD DEK C P P P ++ C S C + + CDG+ DC +DE
Sbjct: 1030 SFPCNNGRCVPLHYRCDGVDDCHDNSDEVQCGAFNTSCAPSAFACGHGGGECIPSYWRCDNHNDCVDGSDEQNCSSQAQTSCRADYFTCDNH-MCIPKNWLCDTDNDCGDGSDE--KRCDLGETCSPTQFHCPN-HRCIDLAFVCDGDKDCADGSDESAC-VINCTDSQFKCVGSNKCISNTYRCDGVSDCSDHSDEI-----------------------------------DCPTRPPGMCRQD--------EFQCREDGICIPDSWECDGHPDCLTGSDEHSGCPPRTCPXSRFLCANGNCIFRDWLCDGDNDCRDMSDEKDC--------PTQPFLCPSWQWQCPGHSICVNLSSVCDGISDCPHGTDE 1346 HSP 5 Score: 88.9669 bits (219), Expect = 5.427e-17 Identity = 86/252 (34.13%), Postives = 120/252 (47.62%), Query Frame = 0
Query: 43 PEAQCSRTDFICEDL-ACINFALRCDGKFDCEDRSDEYQ-CPP--SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGC------------GAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEE---TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAV-----ECDYWQFKCRISGLCIDTRRRCDGTNDC--GSDEDEQ 268
P + C T F CE+ CI CDG+ DC + +DE+Q CPP +C + FTC NG CI + RCD + C D TDEI C GA + ++ R D C + +++C + CI + +CD PDC D SDE + C + + +F C + CI++ CDG +DCLD SDE+ C + EC ++ C G CI R CDG DC G DE++Q
Sbjct: 63 PPSTCGSTQFHCENEDVCIPLYWVCDGEEDCSNGADEHQRCPPGRTCSSH-HFTCTNGECIPVEYRCDHSTDCLDGTDEINCRYPVCQQQTCHNGACYNTSQRCDGEIDCR------DASDELNCTQRCLRNEFQCGSGE-CIPRDYVCDHDPDCSDSSDEHS--CSVYQPCKGNEFACSNG-FCINQNWVCDGMADCLDNSDEDGCESSIIRTHECYPNEWACPEDGKCIPLSRVCDGIADCPRGGDENKQ 303 HSP 6 Score: 88.1965 bits (217), Expect = 9.536e-17 Identity = 81/269 (30.11%), Postives = 103/269 (38.29%), Query Frame = 0
Query: 62 FALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC-NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECD---------------EETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMC-------------------------------------------PKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECD-YWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
F C F D QC P C +TQF C NN CI +CDG C D +DE G F +G F +C E QW+CAN +RCI + CD DC D SDED+ C P C +F C+ RCI K C+G+ DC D SDE +C ++ C +F+C + CI R RCDG NDCG + DE++C
Sbjct: 3487 FTCECPDNFYTIQHGDTTQCLPMCS-STQFLCANNEMCIPIWWKCDGQKDCLDGSDEPNTCPQRFCRLGQFQCSDGNCTSSNFICNARQDCPDGSDEDAVLCEHHRCESNQWQCAN-KRCIPESWQCDSLNDCGDNSDEDSSHCARRTCLPGYFKCANGHCIPQSWKCDVDNDCGDYSDEPLQECMGPAYRCDNYTEFDCKTNYRCIPKWAVCNGFDDCRDNSDEQNCESLTCKPSGEFRC-TNHHCIPLRWRCDGHNDCGDNSDEENC 3752 HSP 7 Score: 85.8853 bits (211), Expect = 4.902e-16 Identity = 75/210 (35.71%), Postives = 97/210 (46.19%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQC-PPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN--DCFAVE---CDYWQFKCRISGLCI 249
QCS ++F C+D CI CD DC D SDE C +C P F C++G CI +RCDG C D++DE C P F + G T +EC NH C+ CDG DC D SDE+ +C + C +F+C D RCI + C+ DC D SDE +C A C +FKC +G CI
Sbjct: 3756 QCSESEFRCDDQTCIPSRWICDQNNDCGDNSDERDCEVMTCHPG-YFQCSSGHCIPDQMRCDGFADCLDASDEATC-------PTRFPN---------------GAYCPATLFECKNH-VCVQPSWKCDGDNDCGDGSDEELHLCLNITCDLTNRFRC-DNNRCIYRHELCNHEDDCGDGSDEKKENCLAPTPRPCTEGEFKCS-NGHCI 3939 HSP 8 Score: 85.5001 bits (210), Expect = 5.981e-16 Identity = 92/310 (29.68%), Postives = 120/310 (38.71%), Query Frame = 0
Query: 89 TQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNE---IEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMS-CSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEK 393
+F C+NG CI + RCDGT C D +DEIGC P C TQ+ C N CI +CDG DC + +DE P C F C + + CI RCD +DCLD +DE +C C Q C +G C +T +RCDG DC DE +C F I P + E C + P ++G M+ C + + E CE+ + C N+W C DG CI L + CD DC G DE
Sbjct: 31 NEFRCSNGHCITESWRCDGTRDCLDGSDEIGCP--------------------------PSTCGS-TQFHCENEDVCIPLYWVCDGEEDCSNGADEHQRCPPGRTCSSH-HFTCTNGE-CIPVEYRCDHSTDCLDGTDEINCRYPVCQ--QQTCH-NGACYNTSQRCDGEIDCRDASDELNCTQRCLRNEFQCGSGECIPRDYVCDHDPDCSDSSDEHSCSVYQPCKGNEFACSNGFCINQNWVCDGMADCLDNSD-----EDGCESS--IIRTHECYPNEWACPEDGKCIPLSRVCDGIADCPRGGDEN 301 HSP 9 Score: 82.4185 bits (202), Expect = 5.758e-15 Identity = 83/250 (33.20%), Postives = 109/250 (43.60%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC-NNGRCIGQDLRCDGTPQCQDSTDEIG-CGAISKVAPGFFSSLNGRIIGLTFDPCVPGE--CDEET----------------QWECANHRRCIDKRRLCDGYPDCPDQSDED--AEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE-CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDN 272
+C +F C + CI + RCDG DC D SDE CPPS +TQF C N CI CDG C + DE C + F+ NG C+P E CD T Q + ++ C + + CDG DC D SDE + C + E FQC + CI + CD DC D SDE+ C + C +F C +G CI+ CDG DC + DE C++
Sbjct: 27 ECLGNEFRCSNGHCITESWRCDGTRDCLDGSDEIGCPPSTCGSTQFHCENEDVCIPLYWVCDGEEDCSNGADEHQRCPPGRTCSSHHFTCTNGE--------CIPVEYRCDHSTDCLDGTDEINCRYPVCQQQTCHNGACYNTSQRCDGEIDCRDASDELNCTQRCLRNE------FQCGSGE-CIPRDYVCDHDPDCSDSSDEHSCSVYQPCKGNEFACS-NGFCINQNWVCDGMADCLDNSDEDGCES 260 HSP 10 Score: 78.9518 bits (193), Expect = 6.610e-14 Identity = 57/175 (32.57%), Postives = 75/175 (42.86%), Query Frame = 0
Query: 54 CEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET------------------QWECANHRRCIDKRRLCDGYPDCPDQSDE-------DAEMCPKVECGEEWQFQCR 203
C + AC N + RCDG+ DC D SDE C C N +F C +G CI +D CD P C DS+DE C F+ NG I + +C + + +W C +CI R+CDG DCP DE D MCP + C ++QC
Sbjct: 153 CHNGACYNTSQRCDGEIDCRDASDELNCTQRCLRN-EFQCGSGECIPRDYVCDHDPDCSDSSDEHSCSVYQPCKGNEFACSNGFCINQNWVCDGMADCLDNSDEDGCESSIIRTHECYPNEWACPEDGKCIPLSRVCDGIADCPRGGDENKQGRVCDVNMCPSLGC----EYQCH 322 HSP 11 Score: 72.0182 bits (175), Expect = 8.736e-12 Identity = 81/251 (32.27%), Postives = 101/251 (40.24%), Query Frame = 0
Query: 199 QFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDE-QDC---DNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVE-PQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
+F+C + CI + RCDG DCLD SDE C C QF C +CI CDG DC + DE Q C + + F TN PV + + D+ P + C + C + C D EI C D + Q C N++QC G CI CD DC D DE CS+ P N EF C +G C + CDGM DC D SDE C
Sbjct: 32 EFRCSNGH-CITESWRCDGTRDCLDGSDEIGCPPSTCGSTQFHCENEDVCIPLYWVCDGEEDCSNGADEHQRCPPGRTCSSHHFTCTNGECIPVEYRCDHSTDCLDGTDEINCRYPVCQQQTC------HNGACYNTSQRC--DGEID------CRDASDELNCTQRCLRNEFQCGSGECIPRDYVCDHDPDCSDSSDEHSCSVYQ----PCKGN-----EFACSNGFCINQNWVCDGMADCLDNSDEDGC 258 HSP 12 Score: 58.9214 bits (141), Expect = 9.036e-8 Identity = 52/152 (34.21%), Postives = 68/152 (44.74%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP-PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGE 196
CS T+F C + CI RCD + DC D SDE C P+CD N FTC NG C + CDG C D++DE+ + C P Q+ C N+ CI+ ++C+ DC D SDE E C EC +
Sbjct: 2989 NCSGTEFRCSNGLCIPNWFRCDRRNDCGDYSDERNCKYPACDENL-FTCQNGICTYKSYICDGENDCGDNSDEL-----------------EHLCHKEETTCPP------HQFRC-NNGNCIEMVKVCNHQADCSDNSDE--ERCGVNECND 3113
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. SwissProt
Match: gi|8928354|sp|Q98930.1|SORL_CHICK (RecName: Full=Sortilin-related receptor; AltName: Full=Low-density lipoprotein receptor relative with 11 ligand-binding repeats; Short=LDLR relative with 11 ligand-binding repeats; Short=LR11; AltName: Full=SorLA-1; AltName: Full=Sorting protein-related receptor containing LDLR class A repeats; Short=SorLA) HSP 1 Score: 124.405 bits (311), Expect = 1.888e-28 Identity = 138/434 (31.80%), Postives = 173/434 (39.86%), Query Frame = 0
Query: 44 EAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPS-CDPNTQFTCN-NGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDP--------------CVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECD-YWQFKCRISGLCIDTRRRCDGTNDC--GSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYY-EGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEI--PAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEP 455
E C + C + CIN +CD DC D SDE CP + CD TQF C +G CI +CD C D++DE C A + FS +G I L++ C E + ++C N CI +R CDG DC D SDED +C K G FQC + CI + C+G +DC D SDE DC C Y F C+ C+ CDG C GSDED E F T C F + VC + C + Y E CE D P Q+QC +G CI + +CD + DC D DEK C + I PP C P + F C G+C CDG DC D SDE CP + S P
Sbjct: 982 ENTCLPNQYRCFNGNCINSIWQCDNNNDCGDMSDEKNCPTTVCDAETQFRCRESGTCIPLSYKCDLEDDCGDNSDESHCEA-HQCRKDEFSCSSGMCIRLSWRCDDDNDCRDWSDEANCTMFRTCEASSFQCLNG-HCIPQRWACDGDADCQDGSDEDPTICEKKCNG----FQCPNGT-CISTSKHCNGITDCADASDEQDCEIPLCTRYMDFVCKNRQQCLSHSMVCDGDIQCEDGSDEDANYAGCAQEPEFHRT-----------------------CDQFSFQCANGVCIS-----------LVWKCDGMDDCGDYSDEASCENPTDA--PTCSRYYQFQCGNGHCIPNQWKCDGENDCGDWSDEKECEGSPLLPITTAVPPTCLP-NHFRCGSGACITNSWVCDGYRDCADGSDEDACPTSHPNVTSSP 1371 HSP 2 Score: 110.923 bits (276), Expect = 4.337e-24 Identity = 129/431 (29.93%), Postives = 175/431 (40.60%), Query Frame = 0
Query: 48 SRTDFIC-EDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRII--------------GLTFDPCVPGECDEE-TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFA---------VECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSI---ETIDEIPAPPNCDPLDEFTCKD-GSCRPIDTRCDGMVDCEDESDEFDCPLGST 449
+ T F C E CI + +CD + DC D SDE C +F+C++G CI RCD C+D +DE C F LNG I G DP + C+++ ++C N CI + C+G DC D SDE + C C F C+++Q+C+ CDG C D SDE+ +A CD + F+C +G+CI +CDG +DCG DE C+N T D PT C + CG +G N+ + + D + EG P C N ++C G+CI CD DC DG DE C PAP EF C+ C P RCDG DC+D +DE CP S+
Sbjct: 1026 AETQFRCRESGTCIPLSYKCDLEDDCGDNSDESHCEAHQCRKDEFSCSSGMCIRLSWRCDDDNDCRDWSDEANCTMFRTCEASSFQCLNGHCIPQRWACDGDADCQDGSDEDPTI---CEKKCNGFQCPNG-TCISTSKHCNGITDCADASDE--QDCEIPLCTRYMDFVCKNRQQCLSHSMVCDGDIQCEDGSDEDANYAGCAQEPEFHRTCDQFSFQC-ANGVCISLVWKCDGMDDCGDYSDEASCENPT-----------------------------DAPT-CSRYYQFQCG-NGHCIPNQWKCDGENDCGDWSDEKECEGSPLLPITTAVPPTCLPNHFRCGSGACITNSWVCDGYRDCADGSDEDACPTSHPNVTSSPPAPRGRCSRTEFECQQLHKCIPNWKRCDGRRDCQDGTDERSCPTHSS 1418 HSP 3 Score: 102.449 bits (254), Expect = 2.311e-21 Identity = 82/248 (33.06%), Postives = 105/248 (42.34%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQC-----PPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRR-----CIDKRRLCDGYPDCPDQSDEDA-----------EMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC---FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
C + F C + CI+ +CDG DC D SDE C P+C QF C NG CI +CDG C D +DE C S + P VP C NH R CI +CDGY DC D SDEDA P+ C +F+C+ +CI +RCDG DC D +DE C ++ C ++C C+ RCDG DC DE++C
Sbjct: 1232 CDQFSFQCANGVCISLVWKCDGMDDCGDYSDEASCENPTDAPTCSRYYQFQCGNGHCIPNQWKCDGENDCGDWSDEKECEG-SPLLP--------------ITTAVPPTC-------LPNHFRCGSGACITNSWVCDGYRDCADGSDEDACPTSHPNVTSSPPAPRGRCSRT-EFECQQLHKCIPNWKRCDGRRDCQDGTDERSCPTHSSLSCPQ-GYRCEDGEACLLATERCDGYLDCSDGSDERNC 1455
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. SwissProt
Match: gi|296452912|sp|Q92673.2|SORL_HUMAN (RecName: Full=Sortilin-related receptor; AltName: Full=Low-density lipoprotein receptor relative with 11 ligand-binding repeats; Short=LDLR relative with 11 ligand-binding repeats; Short=LR11; AltName: Full=SorLA-1; AltName: Full=Sorting protein-related receptor containing LDLR class A repeats; Short=SorLA; Flags: Precursor) HSP 1 Score: 123.25 bits (308), Expect = 6.262e-28 Identity = 151/474 (31.86%), Postives = 199/474 (41.98%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPP---SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDE--IGCGAISKVAPGFFSSLNGRIIGLT-----FDPCVPGE--------CDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMC-----PKV-ECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCF----AVECD-YWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVC--------GTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEE-DPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDG-SCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEPEPTTADPFEYEEELGTWFEGDDYLT 480
QC ++ C CI + CDG DC D SDE C +C+ + F C NG CI Q CDG CQD +DE + C K GF NG I + C G C + C N ++C+ +CDG C D SDEDA P+ + +E+ FQC++ CI +CDG DC D SDE +C A C Y+QF+C +G CI R +CD NDCG DE+DC + PF P P+T P C + M VC G+D E C L A ++ Q+ G C+ E + +P+ C N W+ +CD DC+DG DE C + + EF C+DG +C + RCDG +DC DESDE C T + TAD F GD LT
Sbjct: 1157 QCRSDEYNCSSGMCIRSSWVCDGDNDCRDWSDEANCTAIYHTCEA-SNFQCRNGHCIPQRWACDGDTDCQDGSDEDPVNC---EKKCNGF-RCPNGTCIPSSKHCDGLRDCSDGSDEQHCEPLCTHFMDFVCKNRQQCLFHSMVCDGIIQCRDGSDEDAAFAGCSQDPEFHKVCDEFGFQCQNGV-CISLIWKCDGMDDCGDYSDEANCENPTEAPNCSRYFQFRCE-NGHCIPNRWKCDRENDCGDWSDEKDCGDSHILPF------STPGPSTCLPNYYR------CSSGTCVMDTWVCDGYRDCADGSD----EEACPLLANVTAASTPTQL---GRCDRFEFECHQPKTCIPN-WK-----------RCDGHQDCQDGRDEANCPTHSTLTCMS-------REFQCEDGEACIVLSERCDGFLDCSDESDEKACSDELTVYKVQNLQWTAD-----------FSGDVTLT 1574 HSP 2 Score: 115.161 bits (287), Expect = 2.494e-25 Identity = 134/440 (30.45%), Postives = 173/440 (39.32%), Query Frame = 0
Query: 44 EAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPS-CDPNTQFTCN-NGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYW--QFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSC--------SSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEPEPTTADPFEYEEELGT 471
E C R + C + CIN CD DC D SDE CP + CD +TQF C +G CI +CD C D++DE C +C + ++ C++ CI +CDG DC D SDE A E FQCR+ CI +R CDG +DC D SDE+ V C+ F+C +G CI + + CDG DC DEQ C EP+ C VC +C +M C SD++ +DP + C +QC +G CI L +CD DC D DE C T PNC +F C++G C P +CD DC D SDE DC S P P+T P Y GT
Sbjct: 1075 ENTCLRNQYRCSNGNCINSIWWCDFDNDCGDMSDERNCPTTICDLDTQFRCQESGTCIPLSYKCDLEDDCGDNSDE--------------------------SHCEMHQCRSD-EYNCSSGM-CIRSSWVCDGDNDCRDWSDE-ANCTAIYHTCEASNFQCRNGH-CIPQRWACDGDTDCQDGSDED---PVNCEKKCNGFRCP-NGTCIPSSKHCDGLRDCSDGSDEQHC---------------EPL--------------------CTHFMDFVCKN-----RQQCLFHSMVCDGIIQCRDGSDEDAAF-----AGCSQDPEFHKVCDEFGFQCQNGVCISLIWKCDGMDDCGDYSDEANCENPT-----EAPNCSRYFQFRCENGHCIPNRWKCDRENDCGDWSDEKDCGDSHILPFSTPGPSTCLPNYYRCSSGT 1430
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. SwissProt
Match: gi|341942117|sp|O88307.3|SORL_MOUSE (RecName: Full=Sortilin-related receptor; AltName: Full=Gp250; AltName: Full=Low-density lipoprotein receptor relative with 11 ligand-binding repeats; Short=LDLR relative with 11 ligand-binding repeats; Short=LR11; AltName: Full=SorLA-1; AltName: Full=Sorting protein-related receptor containing LDLR class A repeats; Short=mSorLA; Flags: Precursor) HSP 1 Score: 122.865 bits (307), Expect = 7.626e-28 Identity = 144/442 (32.58%), Postives = 189/442 (42.76%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPP---SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDE--IGCGAISKVAPGFFSSLNGRII--------------GLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCP-------KVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCF----AVECD-YWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC--DNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVC--------GTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEE-DPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDG-SCRPIDTRCDGMVDCEDESDEFDC 444
QC +F C CI + CDG DC D SDE C +C+ + F C+NG CI Q CDG CQD +DE + C K GF NG I G C P C + C N ++C+ +CDG C D SDEDA EC +E+ FQC++ CI +CDG DC D SDE +C A C Y+QF C +G CI R +CD NDCG DE+DC ++ +P P P+T P C + M VC G+D E C A S ++ Q G C+ E + +P+ C N W+ +CD DC+DG+DE C + + EF C+DG +C + RCDG +DC DESDE C
Sbjct: 1157 QCRSDEFNCSSGMCIRSSWVCDGDNDCRDWSDEANCTAIYHTCEA-SNFQCHNGHCIPQRWACDGDADCQDGSDEDPVSC---EKKCNGFHCP-NGTCIPSSKHCDGLRDCPDGSDEQHCEPF-CTRFMDFVCKNRQQCLFHSMVCDGIVQCRDGSDEDAAFAGCSQDPEFHKEC-DEFGFQCQNGV-CISLIWKCDGMDDCGDYSDEANCENPTEAPNCSRYFQFHCE-NGHCIPNRWKCDRENDCGDWSDEKDCGDSHVLPSP--------TPGPSTCLPNYFH------CSSGACVMGTWVCDGYRDCADGSD----EEACPSLANSTAASTPTQF---GQCDRFEFECHQPKKCIPN-WK-----------RCDGHQDCQDGQDEANCPTHSTLTCTS-------REFKCEDGEACIVLSERCDGFLDCSDESDEKAC 1549 HSP 2 Score: 105.916 bits (263), Expect = 2.197e-22 Identity = 85/252 (33.73%), Postives = 114/252 (45.24%), Query Frame = 0
Query: 39 QDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQC-----PPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECG------------EEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC---FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
QD +C F C++ CI+ +CDG DC D SDE C P+C QF C NG CI +CD C D +DE CG S V P S G C+P + C++ C+ +CDGY DC D SDE+A CP + + ++F+C ++CI +RCDG+ DC D DE +C + C +FKC CI RCDG DC + DE+ C
Sbjct: 1317 QDPEFHKECDEFGFQCQNGVCISLIWKCDGMDDCGDYSDEANCENPTEAPNCSRYFQFHCENGHCIPNRWKCDRENDCGDWSDEKDCGD-SHVLP---SPTPGP------STCLP------NYFHCSS-GACVMGTWVCDGYRDCADGSDEEA--CPSLANSTAASTPTQFGQCDRFEFECHQPKKCIPNWKRCDGHQDCQDGQDEANCPTHSTLTCTSREFKCEDGEACIVLSERCDGFLDCSDESDEKAC 1549 HSP 3 Score: 104.375 bits (259), Expect = 6.843e-22 Identity = 89/254 (35.04%), Postives = 119/254 (46.85%), Query Frame = 0
Query: 50 TDFIC-EDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRII--------------GLTFDPCVPGECDEE-TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFA---------VECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENP 277
T F C E CI + +CD + DC D SDE C + +F C++G CI CDG C+D +DE C AI F NG I G DP C+++ + C N CI + CDG DCPD SDE + C C F C+++Q+C+ CDG C D SDE+ FA ECD + F+C+ +G+CI +CDG +DCG DE +C+N TE P
Sbjct: 1121 TQFRCQESGTCIPLSYKCDLEDDCGDNSDESHCEMHQCRSDEFNCSSGMCIRSSWVCDGDNDCRDWSDEANCTAIYHTCEASNFQCHNGHCIPQRWACDGDADCQDGSDEDPV---SCEKKCNGFHCPN-GTCIPSSKHCDGLRDCPDGSDE--QHCEPF-CTRFMDFVCKNRQQCLFHSMVCDGIVQCRDGSDEDAAFAGCSQDPEFHKECDEFGFQCQ-NGVCISLIWKCDGMDDCGDYSDEANCENPTEAP 1366 HSP 4 Score: 101.293 bits (251), Expect = 6.269e-21 Identity = 122/404 (30.20%), Postives = 157/404 (38.86%), Query Frame = 0
Query: 90 QFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAV--ECDYWQFKCRISGLCIDTRRRCDGTNDC--GSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLP----EDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSC--------SSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEPEPTTADPFEYEEE-----LGTW 472
Q+ C+NG CI CD C D +DE C CD +TQ+ C CI CD DC D SDE C +C + +F C CI CDG +DC D SDE +C A+ C+ F+C +G CI R CDG DC GSDED C+ N F N +P++ + P E C FC VC +C +M C SD++ +DP + C +QC +G CI L +CD DC D DE C T PNC +F C++G C P +CD DC D SDE DC P P+T P + +GTW
Sbjct: 1082 QYRCSNGNCINSIWWCDFDNDCGDMSDERNCPTTV--------------------------CDADTQFRCQESGTCIPLSYKCDLEDDCGDNSDE--SHCEMHQCRSD-EFNCSSGM-CIRSSWVCDGDNDCRDWSDEANCTAIYHTCEASNFQCH-NGHCIPQRWACDGDADCQDGSDEDPVSCEKKC-NGFHCPNGT--CIPSSKHCDGLRDCPDGSDEQHCEPFCTRFMDFVCKN-----RQQCLFHSMVCDGIVQCRDGSDEDAAF-----AGCSQDPEFHKECDEFGFQCQNGVCISLIWKCDGMDDCGDYSDEANCENPT-----EAPNCSRYFQFHCENGHCIPNRWKCDRENDCGDWSDEKDCGDSHVLPSPTPGPSTCLPNYFHCSSGACVMGTW 1436
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. nr
Match: gi|1325291962|ref|XP_023329990.1| (uncharacterized protein LOC111702502 [Eurytemora affinis]) HSP 1 Score: 186.808 bits (473), Expect = 5.396e-47 Identity = 107/253 (42.29%), Postives = 135/253 (53.36%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEV 299
CS+T+F C D CI+++ RCD K DC+D SDE C C +FTCN+G CI Q RCDG C+D TDEIGC A F+ C+ E W C + RRCI R CDG+PDC D SDE CP C +W F C + +CID RRRCDGY+DC D SDE C ++C WQFKC+ G+C+ RCD DC DE DC + + F I P+T++P +E
Sbjct: 614 CSKTEFTCGDGTCIDYSKRCDKKIDCKDSSDESGCMKPCSLQ-EFTCNDGSCIEQAQRCDGQIHCKDMTDEIGCDA--------------------FNFCLSSE------WMCVDKRRCISSSRRCDGFPDCGDVSDEQG--CPPQTCNTKWLFPCSNSSQCIDSRRRCDGYTDCQDRSDERGCRELQCQEWQFKCKQDGVCLHEALRCDSVQDCRDGSDEDDCFSDPASTVFPYTTIP---PSTINPQGLET 834 HSP 2 Score: 72.4034 bits (176), Expect = 1.551e-9 Identity = 55/180 (30.56%), Postives = 71/180 (39.44%), Query Frame = 0
Query: 302 EDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGP-------------------------------------CETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
E +C T C PVCG+D LTY N C C + +Y G CE D +GC+ ++ C DG+CI ++CD+K DC+D DE C C L EFTC DGSC RCDG + C+D +DE C
Sbjct: 521 ESECDTVCVKAWNPVCGSDNLTYSNLCEYNVAQCKNS---DLYMLGTGECSNLQLGTICKRTEFLCQESGKCISFLKTCNGKLDCENGSDEKLCEGCSKTEFTCGDGTCIDYSKRCDKKIDCKDSSDESGCMKP----------CS-LQEFTCNDGSCIEQAQRCDGQIHCKDMTDEIGC 686 HSP 3 Score: 72.4034 bits (176), Expect = 1.676e-9 Identity = 98/337 (29.08%), Postives = 131/337 (38.87%), Query Frame = 0
Query: 153 EETQWECANHRRCIDKRRLCDGYPDCPDQSDEDA-EMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQ-WQCLDGS-CIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCK-DGSCRPIDTRCDGMVDCEDESDEFDC---PLGSTSKPSEPEPTTADPFEYEEELGTWFEGDDYLTEG 482
+ T++ C +CI + C+G DC + SDE E C K E F C D CID +RCD DC D SDE+ C C +F C G CI+ +RCDG C DE CD FC L+ E C K SS + + + ++E PQ C + C + S CI +++CD DC+D DE+ C E +F CK DG C RCD + DC D SDE DC P + + P+T +P E E D+Y+ EG
Sbjct: 577 KRTEFLCQESGKCISFLKTCNGKLDCENGSDEKLCEGCSKTE------FTCGDGT-CIDYSKRCDKKIDCKDSSDESGCMK-PCSLQEFTCN-DGSCIEQAQRCDGQIHCKDMTDEIGCDAFN---------------------------------FC------------LSSEWMCVDKRRCISSSRRCDGFPDCGDVSDEQGCPPQTCNTKWLFPCSNSSQCIDSRRRCDGYTDCQDRSDERGCRELQCQEW----------QFKCKQDGVCLHEALRCDSVQDCRDGSDEDDCFSDPASTVFPYTTIPPSTINPQGLETE-----SVDEYIDEG 844
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. nr
Match: gi|1238883829|ref|XP_022254653.1| (low-density lipoprotein receptor-related protein 1B-like, partial [Limulus polyphemus]) HSP 1 Score: 159.458 bits (402), Expect = 1.939e-37 Identity = 144/411 (35.04%), Postives = 193/411 (46.96%), Query Frame = 0
Query: 43 PEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC-DNITENPFFSTNE--IEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVE-PQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGST 449
P +CS T F C D +CI+ CDG+ DC D SDE C + +TQFTCN+G CI + CDGT C+D +DE C +E Q++C + R CID+R+ CDG DC D SDE C ++ QFQC D CID++++CDG DC D SDE +C +C QF C G CI+ R++CDG DC DEQ+C ++ E+ F + I+E + E +C C + DG + + R C D V + C +Q+ C DG+CI +Q+CD +YDCRD DE+ C E+ P FTCKDGSC RCDG VDC D SDE +C S+
Sbjct: 304 PSKRCSSTQFTCNDGSCIDIRRHCDGRVDCNDFSDEVDCGTARCSSTQFTCNDGSCIDERKHCDGTVDCRDFSDEADCDC------------------------------QEGQFQCGD-RSCIDERQKCDGRYDCRDYSDELNCAC------QQDQFQCGDGS-CIDEKQKCDGRHDCRDYSDEQNCVE-DCQEDQFTCG-DGTCINERQKCDGRYDCRDYSDEQNCVEDCREDQFRCGDGTCIDERQKCDGQYDCRDYSDEQNCEVAC--LPGHFTCKDGSCIDGQKRCDGRV-------------DCFDFSDEVNCVEDCREDQFTCGDGTCIDKRQKCDGRYDCRDYSDEQNC------EVACLPG-----HFTCKDGSCIDGRKRCDGRVDCFDFSDEVNCGCSSS 648 HSP 2 Score: 140.198 bits (352), Expect = 4.587e-31 Identity = 144/485 (29.69%), Postives = 193/485 (39.79%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEPEPTTADPFEYEEELGTWFEGDDYLTEGVDTQEPEDPTTTPEPENPTTPYMEPTTDGVVSGGDRHQLQEAQKCSG 529
+C+ F C D CI+ + RCD FDC D SDE +C P Q+ C +G C+ RCDG C D DE+GCG S E +++C + C+D R+ CD DC D SDE E C + + CRD CI + +RC+G DC D DE C + C QF C+ G CID RR CDG DC DE DC+ S+ T + + C + C + + + + E + DP + C+ Q+ C DGSCI +++ CD + DC D DE C P +FTC DGSC I CDG VDC D SDE DC T++ S + T D G+ + + VD ++ D E G GDR + E QKC G
Sbjct: 31 GKCTSDQFQCGDGRCISISARCDKTFDCSDGSDEAECRPVLCRFDQYRCTDGTCLNIAERCDGNADCPDGEDEVGCGICS-----------------------------EQEFQCKDGT-CLDFRKRCDSNIDCGDGSDE--ESCGVISACKPGDVICRDGS-CISQSKRCNGIPDCSDGIDEEKCDSSRCSPRQFTCK-DGSCIDIRRHCDGRVDCNDFSDEVDCE----------------------------------------TSKRCSSTQFMCNDGSCVDRRKHCDGTVDCRDFSD---EVDCDP--SKRCSSTQFTCNDGSCIDIRRHCDGRVDCNDFSDEVGCD---------PSKRCSSTQFTCNDGSCIDIRRHCDGRVDCNDFSDEVDC---GTARCSSTQFTCND--------GSCIDERKHCDGTVDCRDFSDEADCDCQE------------GQFQCGDRSCIDERQKCDG 404 HSP 3 Score: 134.42 bits (337), Expect = 3.166e-29 Identity = 133/405 (32.84%), Postives = 176/405 (43.46%), Query Frame = 0
Query: 53 ICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECG--EEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE-CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSM----SRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTS 450
+C + CI+ +CDG DC+D SDE CP C + QF C +GRCI RCD T C D +DE C R + FD Q+ C + C++ CDG DCPD DE V CG E +FQC+D C+D R+RCD DC D SDE C + C CR G CI +RC+G DC DE+ CD+ +P T + + I DC F + S+ T + + C + C + + + + E + DP + C+ Q+ C DGSCI +++ CD + DC D DE C P +FTC DGSC I CDG VDC D SDE DC S
Sbjct: 1 MCGNGYCIDSRRKCDGYNDCQDSSDERDCPGKCTSD-QFQCGDGRCISISARCDKTFDCSDGSDEAEC----------------RPVLCRFD-----------QYRCTDG-TCLNIAERCDGNADCPDGEDE-------VGCGICSEQEFQCKDGT-CLDFRKRCDSNIDCGDGSDEESCGVISACKPGDVICR-DGSCISQSKRCNGIPDCSDGIDEEKCDSSRCSPRQFTCK-----DGSCIDIRRHCDGRVDCNDFSDEVDCETSKRCSSTQFMCNDGSCVDRRKHCDGTVDCRDFSD---EVDCDP--SKRCSSTQFTCNDGSCIDIRRHCDGRVDCNDFSDEVGCD---------PSKRCSSTQFTCNDGSCIDIRRHCDGRVDCNDFSDEVDCGTARCS 348 HSP 4 Score: 124.405 bits (311), Expect = 6.139e-26 Identity = 144/461 (31.24%), Postives = 186/461 (40.35%), Query Frame = 0
Query: 44 EAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECG-EEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCD-----NITENP------------FFSTNEIEEPVPT--------TVSPISIEVLPEDDCPTFCPSMSRP-VCGTDGLTYENECRLKAMSCSSDQEIQIY-------------YEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC---PLGSTSKPSEPEPTTAD 461
E C F C+D +CI+ RCDG+ DC D SDE C C + QFTC +G CI + +CDG C+D +DE C PG F+ +G CID R+ CDG DC D SDE V CG QFQC +CI+ +CDG DC D SDE +C C + Q+ C SG CID +RCDG DC DE C+ NI P F +E P + P S + P P S +C G++ K + S + Y + P + + P P C +Q C +G CI CD YDC D DE C E CDP ++F C++ C RCDG DC D SDE +C P GS + E + + D
Sbjct: 530 EVACLPGHFTCKDGSCIDGQKRCDGRVDCFDFSDEVNCVEDCRED-QFTCGDGTCIDKRQKCDGRYDCRDYSDEQNCEV--ACLPGHFTCKDGS---------------------------CIDGRKRCDGRVDCFDFSDE-------VNCGCSSSQFQC-PSGKCIELHSKCDGRIDCDDGSDEVNCDT--CLHGQYTC-ASGQCIDRSKRCDGKFDCSDYSDESGCEPVGDVNIRVYPERQTIRQGREVVFRCRDEGSRRGPVEWKRAEQKRLPPGSTDKRGRLTMPNIQPEHSGSYICSAVGISSGTPGAQKVVVLSVQPPVPFYGVNLPQIDPSTYQVDSPSTSTQRP--PGFCKRDQATCQNGQCIRRDFVCDGDYDCTDRSDEVRCGYEL--------QCDP-NQFQCENEHCVLKVWRCDGEDDCGDNSDEKNCVPNPPGSPCRYDEYQCLSGD 938 HSP 5 Score: 70.8626 bits (172), Expect = 5.956e-9 Identity = 66/210 (31.43%), Postives = 88/210 (41.90%), Query Frame = 0
Query: 245 SGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSE 454
+G CID+RR+CDG NDC DE+DC P T++ + IS DC +DG + E ECR P C +Q++C DG+C+ + ++CD DC DGEDE C I + EF CKDG+C RCD +DC D SDE C + S KP +
Sbjct: 4 NGYCIDSRRKCDGYNDCQDSSDERDC------PGKCTSDQFQCGDGRCISISARCDKTFDC-------------SDG-SDEAECR----------------------------PVLCRFDQYRCTDGTCLNIAERCDGNADCPDGEDEVGCGICS------------EQEFQCKDGTCLDFRKRCDSNIDCGDGSDEESCGVISACKPGD 153 HSP 6 Score: 70.0922 bits (170), Expect = 9.899e-9 Identity = 97/326 (29.75%), Postives = 129/326 (39.57%), Query Frame = 0
Query: 40 DDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQF-----TCNNGRCIGQDLRCDGTPQCQ-------------DSTDEIGCGAISKVAP-----------GFFSSLNG--RIIGLTFDPCVP---------------------------GECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQ-----FQCRDKQRCIDKRRRCDGYSDCLDESDENDCF----AVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCD--NITENPFFSTNEIE-EPVPTTVSPI 295
D++ C + C CI+ + RCDGKFDC D SDE C P D N + T GR + R +G+ + STD+ G + + P G S G +++ L+ P VP G C + Q C N + CI + +CDG DC D+SDE V CG E Q FQC + + C+ K RCDG DC D SDE +C C Y +++C CI CDG DC DE C IT+ P S N E + V T + I
Sbjct: 675 DEVNCDTCLHGQYTCASGQCIDRSKRCDGKFDCSDYSDESGCEPVGDVNIRVYPERQTIRQGREVVFRCRDEGSRRGPVEWKRAEQKRLPPGSTDKRGRLTMPNIQPEHSGSYICSAVGISSGTPGAQKVVVLSVQPPVPFYGVNLPQIDPSTYQVDSPSTSTQRPPGFCKRD-QATCQNGQ-CIRRDFVCDGDYDCTDRSDE-------VRCGYELQCDPNQFQC-ENEHCVLKVWRCDGEDDCGDNSDEKNCVPNPPGSPCRYDEYQCLSGDQCIPKSFECDGEFDCQDKSDEIGCTTPTITKPPSESVNVAEGDTVTITCTAI 990
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. nr
Match: gi|1211404976|ref|XP_021470125.1| (low-density lipoprotein receptor-related protein 1-like [Oncorhynchus mykiss]) HSP 1 Score: 159.073 bits (401), Expect = 4.008e-37 Identity = 143/432 (33.10%), Postives = 204/432 (47.22%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDE-YQCPP-SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDEE--------TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE--NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCP-TFCPSMSR-PVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQ-----GCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDE-------FDCP 445
+ C+ + F+C++ CI F +CD + DC D SDE +CP C P QF C G C CDG CQD +DE CG S + S + R I F D C GE + + TQ++CA +RC+ + +CD DC D SDE A C ++ CG + +F+C+D RCI R +CDG DC D SDE +C C+ +QF+C+ + C+ R +CD NDCG + DE C + ++ V+P + D C PS+ R V T+ L++ S S +++ + PQ C +Q+QC +G CI + +CD DC DG DE+ C I +C PLD+F C + C+P+ +CDG DC D SDE F CP
Sbjct: 3085 SNCTASQFVCKNDKCIPFWWKCDTEDDCGDHSDEPAECPTFKCRPG-QFQCGTGICTNPAYICDGDNDCQDHSDEANCGTYSFMFSVCLSLVVCRCIPGIFRCNGQDNCGEGEDETDCPEVTCAPTQFQCAITKRCVPRVWVCDRDNDCVDGSDEPAN-CTQMTCGVD-EFRCKDSGRCIPARWKCDGEDDCGDSSDEPKEECDERTCEPYQFRCK-NNRCVPGRWQCDYDNDCGDNSDEDKCGMSLCHSCYTI---------LVTPYQLF----DSCVFVLLPSVCRCFVTHTETLSF---------SLSLTHLKRVHACVCVCVRVCVLAPQDGCDLKCDSDQFQCKNGHCIPNRWRCDADADCLDGSDEEKC------HIAVERHC-PLDDFQCNNTLCKPLAWKCDGEDDCGDNSDENPDECRRFQCP 3483 HSP 2 Score: 97.4413 bits (241), Expect = 3.088e-17 Identity = 129/439 (29.38%), Postives = 175/439 (39.86%), Query Frame = 0
Query: 48 SRTDFICEDLACINFALRCDGKFDCEDRSDEYQ--CPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFD--------------PCVPGECDE--------ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCP---KVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQD-CDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENE-------CRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLK-QQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDE------FTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
S DF C + CIN++L CDG C+D+SDE Q C C NGRCIG CDGT C D +DE+ C ++ F +G I + C+P +C T C C CDG DC D SDE CP K++C + F C RCI CD +DC + +DE C C +F+C + CI CDG NDCG DE D C T +P + P P + + DC + C + ENE C K C D + +G E+ E E C +++C +G C+ K +CD ++DC+D DE+P P+C + + C+ G C CD DC D SDE +C
Sbjct: 2304 SVDDFECGNGDCINYSLTCDGMAHCKDKSDEKQSYCANRLCKKGYRRCINGRCIGHMFWCDGTDDCGDHSDELPCN-MTLCKAAEFQCRDGSCISNSSRCDQVVNCEDASDEMNCLPTDCSRYFRLGVKGATFQSCERTTLCYLPSWKCDGNNDCGDFSDE--RNCPDKKKLKCPVNF-FAC-PSGRCIPMSWTCDKENDCENGADETHCDKF-CTSTEFECG-NHRCISNHWVCDGANDCGDKSDEDDRCKTKTCSP----EAFQCPGTHVCVPQRWKCDGDKDCLDGADESIKAGCLYNNTCDENEFMCQNRHCIPKHFVCDHDMDCS---DGSDESPE--CEYPTCGPGEFRCTNGRCLNQKVWECDGEFDCQDRS----------DEVPKNPHCTDSERKCNESAYMCRTGKCLNETLLCDHNDDCGDGSDELNC 2716 HSP 3 Score: 93.9745 bits (232), Expect = 4.343e-16 Identity = 84/249 (33.73%), Postives = 115/249 (46.18%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPS-CDPNTQFTCNNGR---------CIGQDLRCDGTPQCQDSTDEIGCGAISKVA--PGFFSSLNGRIIGLTF-----DPCVPG----ECDE---ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEND----CFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDE 267
C +F C D +CI+ + RCD +CED SDE C P+ C + C +CDG C D +DE C K+ FF+ +GR I +++ + C G CD+ T++EC NH RCI +CDG DC D+SDED + C C E FQC C+ +R +CDG DCLD +DE+ + CD +F C+ + CI CD DC DE
Sbjct: 2383 CKAAEFQCRDGSCISNSSRCDQVVNCEDASDEMNCLPTDCSRYFRLGVKGATFQSCERTTLCYLPSWKCDGNNDCGDFSDERNCPDKKKLKCPVNFFACPSGRCIPMSWTCDKENDCENGADETHCDKFCTSTEFECGNH-RCISNHWVCDGANDCGDKSDED-DRCKTKTCSPE-AFQCPGTHVCVPQRWKCDGDKDCLDGADESIKAGCLYNNTCDENEFMCQ-NRHCIPKHFVCDHDMDCSDGSDE 2627 HSP 4 Score: 80.4925 bits (197), Expect = 6.648e-12 Identity = 77/221 (34.84%), Postives = 98/221 (44.34%), Query Frame = 0
Query: 42 IPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEY--QCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKR---RLCDG------YPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--CD-YWQFKCRISGL 247
IP QC +F C++ CI +CDG DC D SDE C P+ +F C N RCI CDG C + DE ++ P ++ +GR C+P T W C C D+ C G P ++ DED P C + +FQCR CI R RCDG +DC+D SDENDC V CD +F CR S L
Sbjct: 920 IPPPQCQPGEFACKNNRCIQERWKCDGDNDCLDNSDEAPELCHLHTCPSDRFKCQNNRCIPLRWLCDGDNDCGNDEDESNTTCSARTCPPNQYACASGR--------CIP------TSWTCDLDDDCGDRSDEPDSCGGSLSHHTSPLLIEEIDEDIATRPPGGCHVD-EFQCRMDSLCIPMRWRCDGDTDCMDLSDENDCEGVTHMCDPAVKFSCRNSAL 1125 HSP 5 Score: 78.1814 bits (191), Expect = 3.219e-11 Identity = 85/298 (28.52%), Postives = 115/298 (38.59%), Query Frame = 0
Query: 151 CDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYEN-----ECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFD 443
C +EC N CI+ CDG C D+SDE C C + ++ +C + RCI CDG DC D SDE C C +F+CR G CI RCD +C DE +C PT C R G G T+++ C L + C + + + + E + C VN + C G CI + CD++ DC +G DE +C D+ EF C + C CDG DC D+SDE D
Sbjct: 2302 CGSVDDFECGNGD-CINYSLTCDGMAHCKDKSDEKQSYCANRLCKKGYR-RCING-RCIGHMFWCDGTDDCGDHSDELPCNMTLCKAAEFQCR-DGSCISNSSRCDQVVNCEDASDEMNC----------------------------------LPTDCSRYFR--LGVKGATFQSCERTTLCYLPSWKCDGNNDCGDFSD---ERNCPDKKKLKCPVNFFACPSGRCIPMSWTCDKENDCENGADETHC-----DKFCTST------EFECGNHRCISNHWVCDGANDCGDKSDEDD 2545 HSP 6 Score: 77.7962 bits (190), Expect = 3.958e-11 Identity = 83/262 (31.68%), Postives = 113/262 (43.13%), Query Frame = 0
Query: 41 DIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVP--GECD-----------------------EETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKR-RRCDGYSDCLDESDEN----DCFAVE--CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
D + +C F C CI + CD + DCE+ +DE C C +T+F C N RCI CDG C D +DE +P F + CVP +CD +E ++ C N R CI K +CD DC D SDE E C CG +F+C + RC++++ CDG DC D SDE C E C+ + CR +G C++ CD +DCG DE +C
Sbjct: 2468 DKKKLKCPVNFFACPSGRCIPMSWTCDKENDCENGADETHCDKFC-TSTEFECGNHRCISNHWVCDGANDCGDKSDEDDRCKTKTCSPEAFQCPGTHV-------CVPQRWKCDGDKDCLDGADESIKAGCLYNNTCDENEFMCQN-RHCIPKHFVCDHDMDCSDGSDESPE-CEYPTCGPG-EFRCTNG-RCLNQKVWECDGEFDCQDRSDEVPKNPHCTDSERKCNESAYMCR-TGKCLNETLLCDHNDDCGDGSDELNC 2716 HSP 7 Score: 75.485 bits (184), Expect = 2.605e-10 Identity = 72/221 (32.58%), Postives = 91/221 (41.18%), Query Frame = 0
Query: 79 YQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC-----------FAVE-------------CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITE 275
Y PP C P +F C N RCI + +CDG C D++DE AP + C C + +++C N+R CI R LCDG DC + DE C C Q+ C RCI CD DC D SDE D +E C +F+CR+ LCI R RCDG DC DE DC+ +T
Sbjct: 919 YIPPPQCQPG-EFACKNNRCIQERWKCDGDNDCLDNSDE---------AP---------------ELCHLHTCPSD-RFKCQNNR-CIPLRWLCDGDNDCGNDEDESNTTCSARTCPPN-QYACA-SGRCIPTSWTCDLDDDCGDRSDEPDSCGGSLSHHTSPLLIEEIDEDIATRPPGGCHVDEFQCRMDSLCIPMRWRCDGDTDCMDLSDENDCEGVTH 1110 HSP 8 Score: 69.3218 bits (168), Expect = 1.842e-8 Identity = 62/193 (32.12%), Postives = 86/193 (44.56%), Query Frame = 0
Query: 81 CPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCG-AISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC----FAVECDYWQFKCRISGLCIDTRRRCDGTNDC---GSDE 265
C CD + QF C NG CI RCD C D +DE C A+ + P ++C N+ C CDG DC D SDE+ + C + +C F+C++ + C+ ++CDG +C D SDE +C CD +F C +G CI + RC+ NDC GSDE
Sbjct: 3395 CDLKCDSD-QFQCKNGHCIPNRWRCDADADCLDGSDEEKCHIAVERHCP-------------------------LDDFQC-NNTLCKPLAWKCDGEDDCGDNSDENPDECRRFQCPPTRSFRCQNARVCLKVSKQCDGVDNCGDNSDELNCQPPPAQPTCDKDEFLC-ANGRCISSSLRCNFFNDCEDYGSDE 3559 HSP 9 Score: 68.9366 bits (167), Expect = 2.755e-8 Identity = 79/281 (28.11%), Postives = 116/281 (41.28%), Query Frame = 0
Query: 44 EAQCSRTDFICEDLA-CINFALRCDGKFDCEDRSDEYQCPPSCDPNT----QFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGA--------------------ISKVAPGFFSSLNGRIIGLTFDPCVPG-------------------------ECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDA-EMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN--DCFAVECD-YWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
+ C +F C+D CI +CDG+ DC D SDE + CD T QF C N RC+ +CD C D++DE CG + + P L+F + +CD + Q++C N CI R CD DC D SDE+ + + C + FQC + C +CDG DC D SDEN +C +C F+C+ + +C+ ++CDG ++CG + DE +C
Sbjct: 3244 QMTCGVDEFRCKDSGRCIPARWKCDGEDDCGDSSDEPK--EECDERTCEPYQFRCKNNRCVPGRWQCDYDNDCGDNSDEDKCGMSLCHSCYTILVTPYQLFDSCVFVLLPSVCRCFVTHTETLSFSLSLTHLKRVHACVCVCVRVCVLAPQDGCDLKCDSD-QFQCKNGH-CIPNRWRCDADADCLDGSDEEKCHIAVERHCPLD-DFQC-NNTLCKPLAWKCDGEDDCGDNSDENPDECRRFQCPPTRSFRCQNARVCLKVSKQCDGVDNCGDNSDELNC 3518 HSP 10 Score: 68.5514 bits (166), Expect = 3.563e-8 Identity = 125/452 (27.65%), Postives = 159/452 (35.18%), Query Frame = 0
Query: 39 QDDIPEAQCSRTDFICEDLA-CINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC--------NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDED---AEMCPKVECGEEWQFQCRDK---------QRCIDKRRRCDGYSDCLDESDE----------------NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITEN--PF--FSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDE-SDEFDC 444
+ D PE C+ T F C C+ CD DC D SDE P +C TQ TC ++GRCI +CDG C DS+DE ECDE T Q+ C N+ RC+ R CD DC D SDED +C +Q D RC +S L D ++CD QF+C+ +G CI R RCD DC DE+ C E P F N T P++ + EDD CG + +ECR C + + C+ C+ + +QCD +C D DE C PA P CD DEF C +G C RC+ DCED SDE C
Sbjct: 3198 ETDCPEVTCAPTQFQCAITKRCVPRVWVCDRDNDCVDGSDE---PANC---TQMTCGVDEFRCKDSGRCIPARWKCDGEDDCGDSSDE-----------------------------PKEECDERTCEPYQFRCKNN-RCVPGRWQCDYDNDCGDNSDEDKCGMSLCHSCYTILVTPYQLFDSCVFVLLPSVCRCFVTHTETLSFSLSLTHLKRVHACVCVCVRVCVLAPQDGCDLKCDSDQFQCK-NGHCIPNRWRCDADADCLDGSDEEKCHIAVERHCPLDDFQCNN------TLCKPLAWKCDGEDD------------CGDNSDENPDECR--RFQCPPTRSFR------CQNAR------------------VCLKVSKQCDGVDNCGDNSDELNCQ-----PPPAQPTCDK-DEFLCANGRCISSSLRCNFFNDCEDYGSDEIHC 3562
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. nr
Match: gi|1192743728|ref|XP_021002577.1| (low-density lipoprotein receptor-related protein 2-like [Parasteatoda tepidariorum]) HSP 1 Score: 158.303 bits (399), Expect = 5.235e-37 Identity = 143/408 (35.05%), Postives = 186/408 (45.59%), Query Frame = 0
Query: 44 EAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDST--DEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC-DNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYE----GPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
+ QCS +F C D CI RCDG++DC D SDE C P+ QF C+NG CI LRCDG C D + DE GC PC P +QW+C + +CID+ +LCDG DC D + D C C + +F+C D CI ++C+G +C D SDE +C C +F C + CID RRRCDG NDC DE+DC N F ++ +TV + CP S V D +CR CS + I C ED C N ++C DG+CI K +CD K+DCRDG DE C + P C L +FTC+DG+C +CDG+V C+D DE C
Sbjct: 383 DMQCSPDEFRCNDKTCIESDQRCDGRYDCPDYSDEQNCTGVVCPSGQFRCDNGSCITMSLRCDGRQDCPDRSGADEKGC------------------------PCSP------SQWQC-DFGQCIDRSKLCDGRVDCADD-NSDERDCAIPGCSAD-KFRCSDGT-CIPASQKCNGVRECRDGSDERNC---PCPSDKFMC-ANRFCIDLRRRCDGYNDCQDSSDEKDCPGECDANQFRCSDGKACIALSTVCDRKYD----------CPDGSDEV---DCRRPTAQCRRDQYRCSDGMCLNIAQRCDGNVDCPGGEDESGCGTCPENMYRCSDGACINYKLRCDGKFDCRDGSDENNCDFK--------PAC-RLTDFTCRDGTCVNQTIKCDGVVHCQDGDDESIC 730 HSP 2 Score: 155.606 bits (392), Expect = 3.946e-36 Identity = 142/423 (33.57%), Postives = 202/423 (47.75%), Query Frame = 0
Query: 39 QDDIPEAQCSR---TDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDE---ETQWECANHR------RCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDN----ITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLT-YENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
QD E+ CSR +++C + CI RCDG+ C + DE CP C QF C NG+CI + +CDG P C DS+DE+ CG PG +G + ++ +C E E EC ++ CI++RR CDGYPDC D+SDE EC E F+C D CI+ ++RCDG DC D SDE +C C+ +F+C +G CID +C+ DC DE +C++ + E I+ + + E DC + C D + EC + C+ + + +G E + P Q C+ +Q++C DG CI +C+RKYDCRDG DE C IE C P EF C +G C + +C+ + DC+D SDE C
Sbjct: 722 QDGDDESICSRCGVGEYVCNNGQCIESRQRCDGRSHCPNGEDEADCPVECLVG-QFRCKNGQCINERRKCDGRPDCADSSDELDCGV---CKPGDVICRDGSCVDVSKRCDGRNDCPEGIDEANCECQSNEFRCGDGACINERRRCDGYPDCRDRSDESN----CDECAENM-FRCGDGT-CIEIKKRCDGRRDCFDHSDEENCDPKTCNAGEFRCN-NGECIDDSLKCNRNYDCRDGSDEFNCESKSCQLDEFKCKDGECIKSELRCNRRYDCRDGTDEFDCES-------KACQPDEFKCKDGECIKSDLRCNRRYDCR---DGTDEFDCGPEPQQQCSASQFRCNDGQCIDSVLKCNRKYDCRDGSDESNCVIEA---------CQPT-EFRCTNGQCIDLSLKCNRVYDCQDGSDETTC 1113 HSP 3 Score: 139.813 bits (351), Expect = 6.563e-31 Identity = 140/456 (30.70%), Postives = 187/456 (41.01%), Query Frame = 0
Query: 22 MSAVATRALNSSEGLMPQD-DIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYE---NECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETI-----------------------------DEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
+S V R + +G D P AQC R + C D C+N A RCDG DC DE C P + C++G CI LRCDG C+D +DE C F+ +G C+++ CDG C D DE +C + GE + C + Q CI+ R+RCDG S C + DE DC VEC QF+C+ +G CI+ RR+CDG DC DE DC + + +S +DCP G D E NE R +C +++ Y C D CA N ++C DG+CI +K++CD + DC D DE+ C +T DE LDEF CKDG C + RC+ DC D +DEFDC
Sbjct: 593 LSTVCDRKYDCPDGSDEVDCRRPTAQCRRDQYRCSDGMCLNIAQRCDGNVDCPGGEDESGCGTC--PENMYRCSDGACINYKLRCDGKFDCRDGSDENNCDFKPACRLTDFTCRDGT---------------------------CVNQTIKCDGVVHCQDGDDE--SICSRCGVGE---YVCNNGQ-CIESRQRCDGRSHCPNGEDEADC-PVECLVGQFRCK-NGQCINERRKCDGRPDCADSSDELDCGVCKPGDVICRD-------GSCVDVSKRCDGRNDCPE----------GIDEANCECQSNEFRCGDGACINERRRCDGYP-DCRDRSDESNCDECAENMFRCGDGTCIEIKKRCDGRRDCFDHSDEENCDPKTCNAGEFRCNNGECIDDSLKCNRNYDCRDGSDEFNCESKSCQLDEFKCKDGECIKSELRCNRRYDCRDGTDEFDC 993 HSP 4 Score: 125.561 bits (314), Expect = 2.880e-26 Identity = 99/238 (41.60%), Postives = 128/238 (53.78%), Query Frame = 0
Query: 43 PEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP-PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAI--SKVAPGFFSSLNGRIIGLTFDPCVPGECDEE------TQWECANHRRCIDKRRLCDGYPDCPDQSDE-DAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
P+ QCS + F C D CI+ L+C+ K+DC D SDE C +C P T+F C NG+CI L+C+ CQD +DE C S G S ++ ++ + C P DE+ TQ++C N+ C+D R CDG PDC D SDE D + C E F+C D +CID RCDG DC D SDE DC C QF+C ++GLCID + CDG DC DE DC
Sbjct: 1036 PQQQCSASQFRCNDGQCIDSVLKCNRKYDCRDGSDESNCVIEACQP-TEFRCTNGQCIDLSLKCNRVYDCQDGSDETTCARCQPSDFQCGDGSCVDSQVRCDGRNDC-PDRSDEKDCGCTPTQFQC-NNGECLDSRSRCDGIPDCDDGSDERDCQDCDADE------FRCGDG-KCIDDTFRCDGLYDCADYSDEQDCETRLCRPSQFRC-VTGLCIDRAKYCDGLPDCPDRSDELDC 1262 HSP 5 Score: 125.176 bits (313), Expect = 4.431e-26 Identity = 133/442 (30.09%), Postives = 193/442 (43.67%), Query Frame = 0
Query: 30 LNSSEGLMPQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAIS--KVAPGFFSSLNGRIIGLTF------------DPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKV--ECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNIT--ENPFFSTNEIEEPVPTTVSPISIEVLPEDDCP-------TFCP-SMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCP 445
L +P + P + S IC D +CI+ CDG DC DE C + P QF C+ RC+ + RCD P C D TDE+ C S + P F +G+ I L F D + + ++ C++ CI+ C+ DC D SDE + C +V EC + +FQC CI +RCD +DC + DE+ C ++C +F+C CI++ +RCDG DC DEQ+C + F N + +S+ DCP CP S S+ C ++ + C+ D E D P GC+ ++++C DG+CI Q+C+ +CRDG DE+ C P D+F C + C + RCDG DC+D SDE DCP
Sbjct: 170 LRGQSSTVPSNLSPSCR-SDNQMICHDGSCIDSVRICDGVKDCSFGEDEINCGITSCPPGQFQCDGSRCVEERKRCDRRPDCSDRTDELDCPDTSDRECRPDQFRCSDGQCIDLRFKCDRKYDCRDGSDEISYSQTCQRDEFRCSDG-ECINLSLKCNNRYDCRDGSDELS--CERVVPECKSD-EFQCSSSGDCIKFFQRCDFVADCPNGEDESFCQDMQCSPDEFRCN-DKTCIESDQRCDGRYDCPDYSDEQNCTGVVCPSGQFRCDN-------GSCITMSLRCDGRQDCPDRSGADEKGCPCSPSQWQCDFGQCIDRSKLCDGRVDCADDN----------SDERDCAIP-GCSADKFRCSDGTCIPASQKCNGVRECRDGSDERNCPC-------------PSDKFMCANRFCIDLRRRCDGYNDCQDSSDEKDCP 574 HSP 6 Score: 122.479 bits (306), Expect = 3.327e-25 Identity = 139/450 (30.89%), Postives = 191/450 (42.44%), Query Frame = 0
Query: 43 PEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQC------------PPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDE-DAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSC-SSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDC--RDGEDEKYCSI----------ETIDEIP------------------APPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGS 448
PE+ C + C CI+ RCDG DC D SDE C PSC + Q C++G CI CDG C DEI CG S C PG Q++C + RC+++R+ CD PDC D++DE D EC + QF+C D Q CID R +CD DC D SDE ++ C +F+C G CI+ +C+ DC DE C+ + P ++E + + S I+ D CP+ D +E R +C SDQ Y+ P ++E C Q++C +GSCI + +CD + DC R G DEK C + ID A P C D+F C DG+C P +C+G+ +C D SDE +CP S
Sbjct: 132 PES-CRIDELSCSSGECISIEKRCDGTEDCRDGSDEVGCLRGQSSTVPSNLSPSCRSDNQMICHDGSCIDSVRICDGVKDCSFGEDEINCGITS---------------------CPPG------QFQC-DGSRCVEERKRCDRRPDCSDRTDELDCPDTSDRECRPD-QFRCSDGQ-CIDLRFKCDRKYDCRDGSDEIS-YSQTCQRDEFRCS-DGECINLSLKCNNRYDCRDGSDELSCERVV--PECKSDEFQ----CSSSGDCIKFFQRCDFVADCPNGEDESFCQDMQCSPDEFRCNDKTCIESDQRCDGRYDCPDYSDEQNCTGVVCPSGQFRCDNGSCITMSLRCDGRQDCPDRSGADEKGCPCSPSQWQCDFGQCIDRSKLCDGRVDCADDNSDERDCAIPGC-SADKFRCSDGTCIPASQKCNGVRECRDGSDERNCPCPS 541 HSP 7 Score: 120.553 bits (301), Expect = 1.355e-24 Identity = 125/382 (32.72%), Postives = 172/382 (45.03%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQC-PPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCG--AISKVAPGFFSSLNGRII--------------GLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNE--IEEPVPTTVSPISIEVLPEDDCPT-FC-PSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPP 407
C +F C+D CI LRC+ ++DC D +DE+ C +C P+ +F C +G CI DLRC+ C+D TDE CG + + F +G+ I G CV C + T++ C N +CID C+ DC D SDE C + + FQC D C+D + RCDG +DC D SDE DC C QF+C +G C+D+R RCDG DC DE+DC + + F + I++ + E DC T C PS R V G C +A C + C D ++ C Q+ C +G CI Q+CD +DC D DE C+ E I P
Sbjct: 959 CQLDEFKCKDGECIKSELRCNRRYDCRDGTDEFDCESKACQPD-EFKCKDGECIKSDLRCNRRYDCRDGTDEFDCGPEPQQQCSASQFRCNDGQCIDSVLKCNRKYDCRDGSDESNCVIEAC-QPTEFRCTNG-QCIDLSLKCNRVYDCQDGSDE--TTCARC---QPSDFQCGDGS-CVDSQVRCDGRNDCPDRSDEKDC---GCTPTQFQCN-NGECLDSRSRCDGIPDCDDGSDERDCQDCDADEFRCGDGKCIDDTFRCDGLYDCADYSDEQDCETRLCRPSQFRCVTGL--------CIDRAKYCDGLPD--------CPDRSDELDCWACQPGQYVCANGQCIERTQRCDGTFDCSDYSDESDCAPEGGVNIKVYP 1311 HSP 8 Score: 110.538 bits (275), Expect = 1.761e-21 Identity = 122/412 (29.61%), Postives = 168/412 (40.78%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET-QWECANHR-----------RCIDKRRLCDGYPDCPDQSDE-DAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
+C+ F C D CI RCDG+ DC D SDE C P +F CNNG CI L+C+ C+D +DE C + S F +G I +C + T +++C + CI C+ DC D +DE D P+ +C QF+C D Q CID +C+ DC D SDE++C C +F+C +G CID +C+ DC DE C + F + + + +DCP T EC C + C+ D + Q C ++++C DG CI +CD YDC D DE+ C C P +F C G C CDG+ DC D SDE DC
Sbjct: 880 ECAENMFRCGDGTCIEIKKRCDGRRDCFDHSDEENCDPKTCNAGEFRCNNGECIDDSLKCNRNYDCRDGSDEFNCESKS-CQLDEFKCKDGECIKSELRCNRRYDCRDGTDEFDCESKACQPDEFKCKDGECIKSDLRCNRRYDCRDGTDEFDCGPEPQQQCSAS-QFRCNDGQ-CIDSVLKCNRKYDCRDGSDESNCVIEACQPTEFRC-TNGQCIDLSLKCNRVYDCQDGSDETTCARCQPSDFQCGD-------GSCVDSQVRCDGRNDCPDRSDEKDCGCTPTQFQCNNGECLDSRSRCDGIPD--------CDDGSDERDCQDCDADEFRCGDGKCIDDTFRCDGLYDCADYSDEQDCETRL---------CRP-SQFRCVTGLCIDRAKYCDGLPDCPDRSDELDC 1262 HSP 9 Score: 110.538 bits (275), Expect = 1.993e-21 Identity = 95/249 (38.15%), Postives = 123/249 (49.40%), Query Frame = 0
Query: 22 MSAVATRALNSSEGLMPQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
+S R + +G D+ A+C +DF C D +C++ +RCDG+ DC DRSDE C C P TQF CNNG C+ RCDG P C D +DE C +CD + ++ C + +CID CDG DC D SDE + C C QF+C CID+ + CDG DC D SDE DC+A C Q+ C +G CI+ +RCDGT DC DE DC
Sbjct: 1094 LSLKCNRVYDCQDG---SDETTCARCQPSDFQCGDGSCVDSQVRCDGRNDCPDRSDEKDC--GCTP-TQFQCNNGECLDSRSRCDGIPDCDDGSDERDC----------------------------QDCDAD-EFRCGDG-KCIDDTFRCDGLYDCADYSDE--QDCETRLC-RPSQFRCV-TGLCIDRAKYCDGLPDCPDRSDELDCWA--CQPGQYVC-ANGQCIERTQRCDGTFDCSDYSDESDC 1299 HSP 10 Score: 66.2402 bits (160), Expect = 1.733e-7 Identity = 48/150 (32.00%), Postives = 69/150 (46.00%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQC--PPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKV 192
C+R++ C++ CI + CDG FDC DRSDE C C PN +F C+N +C+ + RCDG C D++DE C +P + ++ C + +CI K CDG DC D+SDE P +
Sbjct: 1420 GACARSEATCQNGDCIPWTYACDGDFDCTDRSDELNCDYQYQCQPN-EFQCDNKKCVLKVWRCDGDNDCGDNSDEKSCSPNPAGSPCRYD-----------------------EYRCLSGDQCIPKSFQCDGEFDCQDKSDEIGCASPTI 1545
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. nr
Match: gi|939627003|ref|XP_014300580.1| (PREDICTED: basement membrane-specific heparan sulfate proteoglycan core protein isoform X10 [Microplitis demolitor]) HSP 1 Score: 157.147 bits (396), Expect = 1.381e-36 Identity = 146/421 (34.68%), Postives = 193/421 (45.84%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEY-QCPPSCDPNTQFTC-NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCV-----------PGECDE-ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGE----EWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYY----EGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTC-KDGSCRPIDTRCDGMVDCEDESDEFDC 444
C T+F C D CI + L+C+G DC D SDE QC C P TQF C G+CI RCDG P C D +DE CG + N I +T+ P + P ECD T+ C + + CI RR CDG PDC D SDE +CG EW+ C+ + C+ + +RCDG C D SDE +C +C F+C GLC+D RRRCDG C DE +C N S + + P V IS + + S GT ECR +C I + C ED ++ CA Q+QC++G CI +Q+C+ DC DG DE C T+ P + P +FTC D +C RC+G+ +C D SDE +C
Sbjct: 560 CGATEFRCNDGQCIAYELQCNGVNDCRDGSDENDQCDRGCTP-TQFKCATGGKCIEDIYRCDGHPDCPDRSDE-NCGVENLS--------NSTITHITYSPPIDLPVPGRVTERPTECDTGRTEMRCGDGK-CISLRRKCDGIPDCSDNSDER-------DCGNCAINEWR--CKSGE-CLREHQRCDGLIQCRDGSDEANC-VTKCPSGMFRCN-DGLCLDERRRCDGRPHCRDGSDEINCQNA------SCPDGQLPCANGVC-ISKQFFCDRHVDCHDGSDETNCSGTSPRPPVTECRSNEYTCRDRTCIPLSAVCDGRPDCSYNEDELDCHRCARGQYQCINGDCISGEQKCNGVIDCDDGSDEDGCESSTMPSEHHPHHHCPAGQFTCMSDRTCVHNSARCNGIPECSDASDENNC 950 HSP 2 Score: 132.109 bits (331), Expect = 2.581e-28 Identity = 129/418 (30.86%), Postives = 172/418 (41.15%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAI--SKVAPGFFSSLNGRIIGLTFD--------------PCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDE----QDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
C + +F C + CIN RC+G +C D+SDE CP D QF C CI ++ +CDG C + DE+ C S G F +GR I + C C + Q+ CA+ C+ + C+ DC + DE C E F+C D Q CI +C+G +DC D SDEND C QFKC G CI+ RCDG DC DE ++ N T + I+ PVP V+ + PT C + + DG +C C + C D + CA+N+W+C G C+ Q+CD CRDG DE C + P F C DG C RCDG C D SDE +C
Sbjct: 407 CIQNEFRCRNGQCINSRYRCNGATECYDKSDELNCPCKED---QFECAALYCIPKERQCDGVNDCSNGRDELNCEFYTPSYCLSGQFKCHDGRCINGSLQCDGRSDCPDRSDELNCTVTTCTSD-QFRCADGS-CVSIDKRCNKINDCRNGEDEHQCGCGATE------FRCNDGQ-CIAYELQCNGVNDCRDGSDENDQCDRGCTPTQFKCATGGKCIEDIYRCDGHPDCPDRSDENCGVENLSNSTITHITYSPPIDLPVPGRVT----------ERPTECDTGRTEMRCGDG-----KCISLRRKCDGIPD--------CSDNSDERDCGNCAINEWRCKSGECLREHQRCDGLIQCRDGSDEANCVTKC-----------PSGMFRCNDGLCLDERRRCDGRPHCRDGSDEINC 778 HSP 3 Score: 130.183 bits (326), Expect = 1.121e-27 Identity = 142/463 (30.67%), Postives = 202/463 (43.63%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEI--GCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPG-----ECDE---ETQWECANHRRCIDKRRLCDGYPDCPDQSDED-------------AEMCPKV-------------ECGE-EWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNE---IEEPVPTTVSPISIEVLPEDDCPTF-CPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQG----CAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEPEP 457
+ C F C D CIN +L+CDG+ DC DRSDE C + + QF C +G C+ D RC+ C++ DE GCGA F +G+ I + C G +CD TQ++CA +CI+ CDG+PDCPD+SDE+ P + EC + +C D +CI RR+CDG DC D SDE DC + W+ K SG C+ +RCDG C DE +C + F N+ ++E P + E +C CP P +G+ C K C D+ + + +G ET P+ C N++ C D +CI L CD + DC EDE C C ++ C +G C + +C+G++DC+D SDE C S++ PSE P
Sbjct: 483 SYCLSGQFKCHDGRCINGSLQCDGRSDCPDRSDELNCTVTTCTSDQFRCADGSCVSIDKRCNKINDCRNGEDEHQCGCGATE------FRCNDGQCIAYELQCNGVNDCRDGSDENDQCDRGCTPTQFKCATGGKCIEDIYRCDGHPDCPDRSDENCGVENLSNSTITHITYSPPIDLPVPGRVTERPTECDTGRTEMRCGDG-KCISLRRKCDGIPDCSDNSDERDCGNCAINEWRCK---SGECLREHQRCDGLIQCRDGSDEANCVTKCPSGMFRCNDGLCLDERRRCDGRPHCRDGSDEINCQNASCPDGQLPC--ANGV-----CISKQFFC--DRHVDCH-DGSDETNCSGTSPRPPVTECRSNEYTCRDRTCIPLSAVCDGRPDCSYNEDELDCH-----------RC-ARGQYQCINGDCISGEQKCNGVIDCDDGSDEDGCE--SSTMPSEHHP 911 HSP 4 Score: 117.087 bits (292), Expect = 1.699e-23 Identity = 127/453 (28.04%), Postives = 171/453 (37.75%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDE-DAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAV----------ECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDN-----------------ITENPFFSTNEI------EEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSS--------DQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEP 455
+C F C D C++ RCDG+ C D SDE C + P+ Q C NG CI + CD C D +DE C S P EC ++ C + R CI +CDG PDC DE D C + Q+QC + CI ++C+G DC D SDE+ C + C QF C C+ RC+G +C DE +C+ I ENP E+ E + V + LP P SR + G + C + Q + ++ E PQ C ++ C +G CI CD K+DC DG DE C+ P C+P ++F C + C RCDG DC D SDE +C TS P P
Sbjct: 742 TKCPSGMFRCNDGLCLDERRRCDGRPHCRDGSDEINCQNASCPDGQLPCANGVCISKQFFCDRHVDCHDGSDETNCSGTSPRPP-------------------VTEC-RSNEYTCRD-RTCIPLSAVCDGRPDCSYNEDELDCHRCAR------GQYQCINGD-CISGEQKCNGVIDCDDGSDEDGCESSTMPSEHHPHHHCPAGQFTCMSDRTCVHNSARCNGIPECSDASDENNCEGEDQKGELNLKTYPSDQIIKENPVRQGYEVVFHCRDEGYLRADVRWLRGNNLP-------LPPGSRDINGRLEIPNIQVEHAGPYICEAVGYPPSVPGQRVTVHLEVEKFELSTSRPPQVCKYDEATCSNGDCISKLFVCDGKFDCTDGSDEMRCN---------PHGCEP-NQFRCANKQCVSKIWRCDGDKDCADGSDEENC---GTSPPGSP 1146 HSP 5 Score: 113.235 bits (282), Expect = 3.425e-22 Identity = 137/508 (26.97%), Postives = 188/508 (37.01%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF--------------------DPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDE-DAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAV----------ECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDN-----------------ITENPFFSTNEI--------------------EEPVPTTVSPISIEV-LP----EDDCPTFCPSMSRP--VCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCE--------------------TEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCD-PLDEFTCKD-GSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEP 455
C+ ++ C+ C+ RCDG C D SDE C C P+ F CN+G C+ + RCDG P C+D +DEI C S G NG I F P P ++ C + R CI +CDG PDC DE D C + Q+QC + CI ++C+G DC D SDE+ C + C QF C C+ RC+G +C DE +C+ I ENP E+ P+P I+ + +P E P C ++ P V G +T E +S S ++ Y E C ++E P GC NQ++C + C+ +CD DC DG DE+ C +PP +EF C C P CD DC+D SDE C +KP P
Sbjct: 704 GNCAINEWRCKSGECLREHQRCDGLIQCRDGSDEANCVTKC-PSGMFRCNDGLCLDERRRCDGRPHCRDGSDEINCQNAS-CPDGQLPCANGVCISKQFFCDRHVDCHDGSDETNCSGTSPRPPVTECRSNEYTCRD-RTCIPLSAVCDGRPDCSYNEDELDCHRCAR------GQYQCINGD-CISGEQKCNGVIDCDDGSDEDGCESSTMPSEHHPHHHCPAGQFTCMSDRTCVHNSARCNGIPECSDASDENNCEGEDQKGELNLKTYPSDQIIKENPVRQGYEVVFHCRDEGYLRADVRWLRGNNLPLPPGSRDINGRLEIPNIQVEHAGPYICEAVGYPPSVPGQR-VTVHLEVEKFELSTSRPPQVCKYDEATCSNGDCISKLFVCDGKFDCTDGSDEMRCNPHGCEPNQFRCANKQCVSKIWRCDGDKDCADGSDEENCGT-------SPPGSPCQYNEFRCNQYDQCIPKSYHCDMERDCQDGSDEIGCSPVYITKPPIP 1193 HSP 6 Score: 109.768 bits (273), Expect = 3.579e-21 Identity = 86/228 (37.72%), Postives = 110/228 (48.25%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP-PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDE-DAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
+ CS +F C+ CI RC+ DCED SDE C P+C N +F C NG+CI RC+G +C D +DE+ C PC +E Q+ECA CI K R CDG DC + DE + E C QF+C D RCI+ +CDG SDC D SDE +C C QF+C G C+ +RC+ NDC + EDE C
Sbjct: 366 SSCSENEFNCDVSRCILDKDRCNFIQDCEDGSDERGCKYPACIQN-EFRCRNGQCINSRYRCNGATECYDKSDELNC------------------------PC------KEDQFECA-ALYCIPKERQCDGVNDCSNGRDELNCEFYTPSYC-LSGQFKCHDG-RCINGSLQCDGRSDCPDRSDELNCTVTTCTSDQFRC-ADGSCVSIDKRCNKINDCRNGEDEHQC 558 HSP 7 Score: 89.7373 bits (221), Expect = 8.153e-15 Identity = 67/186 (36.02%), Postives = 88/186 (47.31%), Query Frame = 0
Query: 85 CDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
C T C +G C+ + RCDG PQC D +DE+ C P F N I T C + C++ R I ++CDG PDC D DE C C E +F C D RCI + RC+ DC D SDE C C +F+CR +G CI++R RC+G +C DE +C
Sbjct: 274 CSDPTSLRCRDGSCVPLNYRCDGVPQCPDQSDELDC-------PSFELLENATRILST------NNCRGDDTVRCSDGSRDICSVQVCDGVPDCDDHGDE--RNCDGSSCSEN-EFNC-DVSRCILDKDRCNFIQDCEDGSDERGCKYPACIQNEFRCR-NGQCINSRYRCNGATECYDKSDELNC 441 HSP 8 Score: 70.8626 bits (172), Expect = 6.726e-9 Identity = 93/331 (28.10%), Postives = 120/331 (36.25%), Query Frame = 0
Query: 37 MPQDDIPEAQCSRTDFIC-EDLACINFALRCDGKFDCEDRSDEYQC-----------------------PPSCDPNTQFTCNNGRCIGQD---LRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGE----CDEETQWECANHR---------------RCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCF----AVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVL-PEDDCPTFCPSMSRPV 316
MP + P C F C D C++ + RC+G +C D SDE C P F C + + D LR + P S D G I + +G + P VPG+ E ++E + R CI K +CDG DC D SDE C C E QF+C +KQ C+ K RCDG DC D SDE +C C Y +F+C CI CD DC DE C PV T PI + VL P D C ++ P+
Sbjct: 905 MPSEHHPHHHCPAGQFTCMSDRTCVHNSARCNGIPECSDASDENNCEGEDQKGELNLKTYPSDQIIKENPVRQGYEVVFHCRDEGYLRADVRWLRGNNLPLPPGSRDINGRLEIPNIQVEHAGPYICEAVG--YPPSVPGQRVTVHLEVEKFELSTSRPPQVCKYDEATCSNGDCISKLFVCDGKFDCTDGSDE--MRCNPHGC-EPNQFRCANKQ-CVSKIWRCDGDKDCADGSDEENCGTSPPGSPCQYNEFRCNQYDQCIPKSYHCDMERDCQDGSDEIGC---------------SPVYITKPPIPMVVLEPGDTMILVCTAIGVPI 1214 HSP 9 Score: 65.4698 bits (158), Expect = 2.751e-7 Identity = 80/282 (28.37%), Postives = 113/282 (40.07%), Query Frame = 0
Query: 39 QDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPN----------TQFTCNNGR-CIGQDLRCDGTPQCQDSTDEIGCGA----------------ISKVAP---GF----------FSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCD--GY-PDCPDQSDEDAEMCPKVECGEEWQFQC--RDKQRC-----IDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
+D++ +C+R + C + CI+ +C+G DC+D SDE C S P+ QFTC + R C+ RC+G P+C D++DE C I K P G+ + + R + P PG D + E N + +C+ GY P P Q K E Q D+ C I K CDG DC D SDE C C+ QF+C + C+ RCDG DC DE++C
Sbjct: 858 EDELDCHRCARGQYQCINGDCISGEQKCNGVIDCDDGSDEDGCESSTMPSEHHPHHHCPAGQFTCMSDRTCVHNSARCNGIPECSDASDENNCEGEDQKGELNLKTYPSDQIIKENPVRQGYEVVFHCRDEGYLRADVRWLRGNNLPLPPGSRDINGRLEIPNIQVEHAGPYICEAVGYPPSVPGQRVTVHLEVEKFELSTSRPPQVCKYDEATCSNGDCISKLFVCDGKFDCTDGSDEMRCNPHGCEPNQFRC-ANKQCVSKIWRCDGDKDCADGSDEENC 1138
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. nr
Match: gi|668452382|gb|KFB41335.1| (AGAP003656-PA-like protein [Anopheles sinensis]) HSP 1 Score: 155.992 bits (393), Expect = 3.159e-36 Identity = 146/419 (34.84%), Postives = 191/419 (45.58%), Query Frame = 0
Query: 48 SRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCN-NGRCIGQDLRCDGTPQCQDSTDEIGC----GAISKVAP-GFFS-------SLNGRIIGLTFDPCVPGECDE----ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCD--NITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYEN-ECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQG-CAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCP 445
+ ++ C D CI+ +CDG DC DEY C P CDP +F C + CI +D CDG PQC D DE C G ++ P G F+ S+ R G P + DE + W+C ++ +CI + CDG DCPD D CP+ C QF+CR RCI +RCD + DC D SDE +C EC QF+C +G CID+R RCDG+ C DE +C +N F N P+ DC + C D T N C C+ E + ET + E G C +Q+ C DG C+ + CD + DCRDG DE C P P C ++F C DG C +C+G+VDC DESDE DCP
Sbjct: 490 AEAEYTCRDGTCIDRDRQCDGVPDCRGGDDEYDCEPDCDPVLEFHCEADNTCIPRDNVCDGAPQCTDGADESNCAGKGGRQARFCPLGKFTCGDRSCVSILARCDGKVDCPHDQADEDECPCPKHTWQC-DYGQCIPHEQKCDGNIDCPDDI-SDERNCPQRNCTVH-QFKCRLDNRCIPIAKRCDRHYDCNDYSDEKNC---ECSADQFQC-ANGACIDSRLRCDGSFHCVDRSDELNCQYPKCLDNEFTCDN-------GQCIPLQRRCDNRPDCNDNSDERNCDGCSEDQFTCGNGRCIPMDEMCNGFNECGDNTD---ETNDRCDEFDGTCQPDQFTCYDGDCVSMTAHCDGRIDCRDGSDENGC--------PGPVRCG-ANQFECDDGICIASYKKCNGIVDCHDESDELDCP 882 HSP 2 Score: 147.132 bits (370), Expect = 2.782e-33 Identity = 139/408 (34.07%), Postives = 188/408 (46.08%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP-PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECG-EEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFA-VECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPT---FCPSMSRPVCGTDGLT-YENECRLKAMSCSSDQEIQ-IYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRK-YDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
+CS F C + ACI+ LRCDG F C DRSDE C P C N +FTC+NG+CI RCD P C D++DE C S E Q+ C N RCI +C+G+ +C D +DE + C + + + QF C D C+ CDG DC D SDEN C V C QF+C G+CI + ++C+G DC + DE DC + T+ ++NE + +PE + T FC + C D N+C L C Q E C T E C ++++C +G CI L+ +CD+ DC D DE + +P NC DEF+C DG C +D +CDG DC D DE+DC
Sbjct: 687 ECSADQFQCANGACIDSRLRCDGSFHCVDRSDELNCQYPKCLDN-EFTCDNGQCIPLQRRCDNRPDCNDNSDERNCDGCS-----------------------------EDQFTCGNG-RCIPMDEMCNGFNECGDNTDETNDRCDEFDGTCQPDQFTCYDGD-CVSMTAHCDGRIDCRDGSDENGCPGPVRCGANQFECD-DGICIASYKKCNGIVDCHDESDELDCPDSTD---CNSNEFACDGQCYEKTVYCNAIPECEDGTDEAFCIT-----CQGDAFHCNNNKCILPEYRCDGQQHCSDASDEHGCNTSE------SCRADEFRCKNGVCINLEFRCDKTPRDCLDNSDE--------ENLPPSENCR-ADEFSC-DGRCIALDRQCDGRRDCNDGMDEYDC 1037 HSP 3 Score: 130.954 bits (328), Expect = 5.103e-28 Identity = 135/435 (31.03%), Postives = 187/435 (42.99%), Query Frame = 0
Query: 39 QDDIPEA----QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPC--VPGECDEETQWECANH-----------------RRCIDKRRLCDGYPDCP-DQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDE-SDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNE--IEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYEN-ECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCP 445
+D+ PEA C F C D CI+ + +CD + DC D +DE CP CD ++TC +G CI +D +CDG P C+ DE C F + I + C P D + CA R C+ CDG DCP DQ+DED CPK WQ D +CI ++CDG DC D+ SDE +C C QFKCR+ CI +RCD DC DE++C+ + + F N I+ + S ++ E +C P C + T +N +C C + + C D GC+ +Q+ C +G CI + + C+ +C D DE + D P D+FTC DG C + CDG +DC D SDE CP
Sbjct: 439 EDETPEACGGGVCEPHQFKC-DSKCISLSKKCDRERDCRDGTDEEDCPVECDAEAEYTCRDGTCIDRDRQCDGVPDCRGGDDEYDCEPDCDPVLEFHCEADNTCIPRD-NVCDGAPQCTDGADESNCAGKGGRQARFCPLGKFTCGDRSCVSILARCDGKVDCPHDQADEDECPCPK----HTWQC---DYGQCIPHEQKCDGNIDCPDDISDERNCPQRNCTVHQFKCRLDNRCIPIAKRCDRHYDCNDYSDEKNCE-CSADQFQCANGACIDSRLRCDGSFHCVDRSDELNC-------QYPKCLDNEFTCDNGQCIPLQRRCDNRPD--------CNDNSDERNCDGCSEDQFTCGNGRCIPMDEMCNGFNECGDNTDETNDRCDEFDGTCQP------DQFTCYDGDCVSMTAHCDGRIDCRDGSDENGCP 842 HSP 4 Score: 112.464 bits (280), Expect = 4.851e-22 Identity = 145/495 (29.29%), Postives = 199/495 (40.20%), Query Frame = 0
Query: 31 NSSEGLMPQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNT--QFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHR------------------RCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSD-EDEQDCD----------NITENP----FFSTNEIE--------------------EPVP---------TTVSPISIEVLPEDDC---------PTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKP 452
NS E +P P C +F C D CI +CDG+ DC D DEY CPP P + C +G C RCDG C D DE GC + F+ L+G + C+ ++ ++C + RCI + +LCD DC D SDE C + E F+C Q CID+ R CD DC D SDE DC CD +F+C +G CI RC+ DC D DE DC+ N+ P +NE+ +P+P + I ++ E C P ++S V + + Y N R A+ + I + G +T + P P CA+N+ C++G CI CD K DC DG DE C ++ C+P ++F C + C CDG DC D SDE +CP S P
Sbjct: 992 NSDEENLP----PSENCRADEFSC-DGRCIALDRQCDGRRDCNDGMDEYDCPPPPPPLRCEEHQCPDGSCFRNSQRCDGRRDCPDGYDEAGCRTCNATE---FTCLDGHL-------CIDASLRCDSIYDCKDFSDEQNCFACRPEEFTCGDGRCISRVQLCDRRSDCSDGSDERNCSCARGE------FRCGTGQ-CIDQSRHCDQRIDCADGSDERDC---NCDRNEFRCE-NGPCIPMALRCNRQVDCPYDTSDELDCETEPPSGQTGLNLKTYPDQQSIKESNEVVFQCRDEGPMHAKVKWVRANGQPMPQGTRDMNGRLEIPMIRVDHSGEYICEAVGYPPSTPGSSKTVSLNVERSWNIKYRN-LRNSALLYGTVPHIGFKFFGT-DTYQRP--PTVCAINEATCMNGECIAKTMICDGKRDCSDGSDETSCGLQ---------QCEP-NQFRCNNRKCVLKTWLCDGEQDCGDGSDEENCPTQSPDAP 1446 HSP 5 Score: 109.383 bits (272), Expect = 4.441e-21 Identity = 129/418 (30.86%), Postives = 167/418 (39.95%), Query Frame = 0
Query: 44 EAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP--PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET-----------QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGY-SDCLDESDENDCFAVE-CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTY--ENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
+ C F C D C++ CDG+ DC D SDE CP C N QF C++G CI +C+G C D +DE+ C + F+ + +P EC++ T + C N+ +CI CDG C D SDE C E +F+C++ CI+ RCD DCLD SDE + E C +F C G CI R+CDG DC DE DC E + P + S DCP C T + C ++ C S IY C+ D C ++ C DG CI Q CDR+ DC DG DE+ CS EF C G C CD +DC D SDE DC
Sbjct: 804 DGTCQPDQFTCYDGDCVSMTAHCDGRIDCRDGSDENGCPGPVRCGAN-QFECDDGICIASYKKCNGIVDCHDESDELDCPDSTDCNSNEFACDGQCYEKTVYCNAIP-ECEDGTDEAFCITCQGDAFHC-NNNKCILPEYRCDGQQHCSDASDEHG--CNTSESCRADEFRCKNGV-CINLEFRCDKTPRDCLDNSDEENLPPSENCRADEFSC--DGRCIALDRQCDGRRDCNDGMDEYDCPPPPPP--LRCEEHQCPDGSCFRN-SQRCDGRRDCPDGYDEAGCRTCNATEFTCLDGHLCIDASLRCDS-----IY---DCKDFSDEQNCFACRPEEFTCGDGRCISRVQLCDRRSDCSDGSDERNCSCAR-------------GEFRCGTGQCIDQSRHCDQRIDCADGSDERDC 1189 HSP 6 Score: 94.3597 bits (233), Expect = 2.518e-16 Identity = 101/313 (32.27%), Postives = 134/313 (42.81%), Query Frame = 0
Query: 172 CDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDY-WQFKCRISGLCIDTRRRCDGTNDCGSDEDEQ----DCDNITENPFFSTNE-IEEPVPTTVSPISIEVLPEDDC-------PTFCPSMSRPVCGTDGLTY-----------------ENECRLKAMSCSSDQEIQIYYEGPCE---------TEEDPVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
CDG DCP+ DE E C C E QF+C K CI ++CD DC D +DE DC VECD ++ CR G CID R+CDG DC +DE DCD + E + N I +P + E +C FCP + + CG E+EC + D I +E C+ ++E + C V+Q++C LD CI + ++CDR YDC D DEK C D+F C +G+C RCDG C D SDE +C
Sbjct: 429 CDGKRDCPNGEDETPEACGGGVC-EPHQFKCDSK--CISLSKKCDRERDCRDGTDEEDC-PVECDAEAEYTCR-DGTCIDRDRQCDGVPDCRGGDDEYDCEPDCDPVLEFHCEADNTCIPRDNVCDGAPQCTDGADESNCAGKGGRQARFCP-LGKFTCGDRSCVSILARCDGKVDCPHDQADEDECPCPKHTWQCDYGQCIPHEQKCDGNIDCPDDISDERNCPQRNCTVHQFKCRLDNRCIPIAKRCDRHYDCNDYSDEKNCECSA-------------DQFQCANGACIDSRLRCDGSFHCVDRSDELNC 722 HSP 7 Score: 88.1965 bits (217), Expect = 2.472e-14 Identity = 125/456 (27.41%), Postives = 174/456 (38.16%), Query Frame = 0
Query: 47 CSRTDFICED-LACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDC-LDESDENDC--------------------FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVS-PISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPL-----------------------------------DEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
C+ T+F C D CI+ +LRCD +DC+D SDE C +C P +FTC +GRCI + CD C D +DE C C GE + C + CID+ R CD DC D SDE C + E F+C + CI RC+ DC D SDE DC E + F+CR G + + + N + +D + E P + E + V P S P ++S V + + Y N R A+ + I + G +T + P P CA+N+ C++G CI CD K DC DG DE C ++ + P C+ DE+ C+ G C P +CD DC D+SDE C
Sbjct: 1081 CNATEFTCLDGHLCIDASLRCDSIYDCKDFSDEQNCF-ACRPE-EFTCGDGRCISRVQLCDRRSDCSDGSDERNCS------------------------CARGE------FRCGTGQ-CIDQSRHCDQRIDCADGSDERDCNCDRNE------FRCENGP-CIPMALRCNRQVDCPYDTSDELDCETEPPSGQTGLNLKTYPDQQSIKESNEVVFQCRDEGP-MHAKVKWVRANGQPMPQGTRDMNGRLEIPMIRVDHSGEYICEAVGYPPST--------PGSSKTVSLNVERSWNIKYRN-LRNSALLYGTVPHIGFKFFGT-DTYQRP--PTVCAINEATCMNGECIAKTMICDGKRDCSDGSDETSCGLQQCE--PNQFRCNNRKCVLKTWLCDGEQDCGDGSDEENCPTQSPDAPCRHDEYQCRSGQCIPKSFQCDTHPDCLDQSDEIGC 1481 HSP 8 Score: 72.4034 bits (176), Expect = 2.154e-9 Identity = 57/158 (36.08%), Postives = 74/158 (46.84%), Query Frame = 0
Query: 76 SDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDA--EMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCF 231
+D YQ PP+ + TC NG CI + + CDG C D +DE CG C P Q+ C N+R+C+ K LCDG DC D SDE+ P C + ++QCR Q CI K +CD + DCLD+SDE C
Sbjct: 1355 TDTYQRPPTVCAINEATCMNGECIAKTMICDGKRDCSDGSDETSCG---------------------LQQCEP------NQFRC-NNRKCVLKTWLCDGEQDCGDGSDEENCPTQSPDAPCRHD-EYQCRSGQ-CIPKSFQCDTHPDCLDQSDEIGCM 1482
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. nr
Match: gi|1211275308|ref|XP_021421567.1| (low-density lipoprotein receptor-related protein 1-like [Oncorhynchus mykiss]) HSP 1 Score: 155.606 bits (392), Expect = 4.989e-36 Identity = 143/425 (33.65%), Postives = 199/425 (46.82%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDE-YQCPP-SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDEE--------TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE--NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDE-------FDCP 445
+ C+ + F+C++ CI F +CD + DC DRSDE +CP C P QF C G C CDG CQD++DE C + F S R I F D C GE +++ TQ++CA +RCI + +CD DC D SDE A C ++ CG + +F+C+D RCI R +CDG +DC D SDE +C C+ +QF+C+ + C+ R +CD NDCG + DE C VP RP ++ C C D + +G ++ED E + C +Q+QC +G CI + +CD DC DG DE+ C I AP +C PLD+F C + C+P+ +CDG DC D SDE F CP
Sbjct: 3355 SNCTASQFVCKNDKCIPFWWKCDTEDDCGDRSDEPAECPAFKCRPG-QFQCGTGICTNPAYICDGDNDCQDNSDEANCDIHVCLPSQFKCSHPSRCIPGIFRCNGQDNCGEGEDEKDCPEVTCAPTQFQCAITKRCIPRVWVCDRDNDCVDGSDEPAN-CTQMTCGVD-EFRCKDSGRCIPARWKCDGENDCGDSSDEPKEECDERTCEPYQFRCK-NNRCVPGRWQCDYDNDCGDNSDEDKC-----------------VP------------------------RPCSESEFACTNGRCIAGRWKCDGDHDCA---DG---SDEDGCELR-CDSDQFQCKNGHCIPSRWRCDADADCLDGSDEEKCHI-------APKHC-PLDDFQCNNTLCKPLAWKCDGEDDCGDNSDENPDQCRMFQCP 3719 HSP 2 Score: 111.694 bits (278), Expect = 1.034e-21 Identity = 123/410 (30.00%), Postives = 161/410 (39.27%), Query Frame = 0
Query: 42 IPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEY--QCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFD---------------PCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDED--AEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--------CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCE--TEEDPVEPQGCAVNQWQCL--DGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCD--PLDEFTC 417
IP QC +F C + CI +CDG DC D SDE C P+ +F C N RCI CDG C DE ++ P ++ +GR I ++ C C TQ+ CAN R CI+ CD DC D SDE + C V QF+C + RCI + CDG +DC D SDE C +F+CR+ GLCI R RCDG DC DE DC+ +T C + C C KA C D + CE ++ED E C ++ C+ D C+ +++ CD DC DG DEK C + +ID NC P + F C
Sbjct: 875 IPPPQCQLGEFSCNNNRCIQERWKCDGDNDCLDNSDEAPELCHQHTCPSDRFKCQNNRCIPLRWLCDGDNDCGTYEDESNTTCSARTCPPNQYACASGRCIPTSWTCDLDDDCGDRSDEPASCAYPTCFPLTQFTCANGR-CINVNWRCDNDNDCGDNSDEAGCSHSCSSV------QFKC-NSGRCIPEYWTCDGDNDCGDYSDETHANCTNQATRPPGGCHVDEFQCRMDGLCIPMRWRCDGDTDCMDLSDESDCEGVTH--------------------------------MCDPAVKFSCRNSA-----RCISKAWVCDGDSD--------CEDNSDEDNCEALVCKLSHHVCVTNDSICLPVEKLCDGTDDCPDGSDEKLCDLCSIDNGGCSHNCSIIPGEGFMC 1231 HSP 3 Score: 105.145 bits (261), Expect = 1.351e-19 Identity = 118/415 (28.43%), Postives = 177/415 (42.65%), Query Frame = 0
Query: 43 PEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNN-GRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCV-----PGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNE---IEEPVPTTVSPISIEVLPEDDC---PTFCP--------SMSRPV---------CGTDGLTYENECRL------KAMSCSSDQEIQIYYEGPCETEED-------------PVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRD-GEDEKYCSIET 399
P +C F C C N A CDG DC+D SDE C +QF C++ RCI RC+G C + DE C ++ F ++ R I + + CV P C + T ++ C + RCI R CDG DC D SDE E C + C E +QF+C++ RC+ R +CD +DC D SDE+ C C +F C +G CI R +CDG +DC DE C+ ++ F I ++ E+ C P CP ++ +P+ CG + ++CR+ + C +D+ + + C+ ++ P EP C +++ C +G CI +C+ DC D G DE C +T
Sbjct: 3393 PAFKCRPGQFQCGTGICTNPAYICDGDNDCQDNSDEANCDIHVCLPSQFKCSHPSRCIPGIFRCNGQDNCGEGEDEKDCPEVTCAPTQFQCAITKRCIPRVWVCDRDNDCVDGSDEPANCTQMTCGVDEFRCKDSGRCIPARWKCDGENDCGDSSDEPKEECDERTC-EPYQFRCKN-NRCVPGRWQCDYDNDCGDNSDEDKCVPRPCSESEFAC-TNGRCIAGRWKCDGDHDCADGSDEDGCELRCDSDQFQCKNGHCIPSRWRCDADADCLDGSDEEKCHIAPKHCPLDDFQCNNTLCKPLAWKCDGEDDCGDNSDENPDQCRMFQCPPTRTFRCQNDR-VCLQVSKQCDGVDNCGDNSDELNCQPPPAEPT-CEKDEFLCANGGCISSSLRCNFFNDCEDYGSDEIQCKTDT 3802 HSP 4 Score: 97.0561 bits (240), Expect = 4.325e-17 Identity = 114/382 (29.84%), Postives = 157/382 (41.10%), Query Frame = 0
Query: 115 TDEIGCGAISKVAPG----------FFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPF-FSTNEIEEPVPTT-VSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTY-------------------------------ENECRLKAMSCSSDQ----EIQIYYEGPC--ETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLG 447
TD GC + ++PG F+ + +GR C +Q+ C N + CI CD DC D+SDE AE CP +C QFQC C + CDG +DC D SDE +C C QFKC CI RC+G ++CG EDE+DC +T P F + +P V + + D P C M+ CG D E C C +++ Q Y+ C ++ED P+ C+ +++ C +G CI + +CD +DC DG DE C + CD D+F CK+G C P RCD DC D SDE C +
Sbjct: 3319 TDNGGCSNLCLLSPGGGYKCACPTNFYLANDGRTC---MSNCTA------SQFVCKNDK-CIPFWWKCDTEDDCGDRSDEPAE-CPAFKC-RPGQFQC-GTGICTNPAYICDGDNDCQDNSDEANCDIHVCLPSQFKCSHPSRCIPGIFRCNGQDNCGEGEDEKDCPEVTCAPTQFQCAITKRCIPRVWVCDRDNDCVDGSDEPANCTQMT---CGVDEFRCKDSGRCIPARWKCDGENDCGDSSDEPKEECDERTCEPYQFRCKNNRCVPGRWQCDYDNDCGDNSDEDKCVPRPCSESEFACTNGRCIAGRWKCDGDHDCADGSDEDGCELR----------CDS-DQFQCKNGHCIPSRWRCDADADCLDGSDEEKCHIA 3673 HSP 5 Score: 97.0561 bits (240), Expect = 4.518e-17 Identity = 128/438 (29.22%), Postives = 174/438 (39.73%), Query Frame = 0
Query: 48 SRTDFICEDLACINFALRCDGKFDCEDRSDEYQ--CPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFD--------------PCVPGECDE--------ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCP---KVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYY----EGPCE--TEEDP-VEPQGCAVNQWQCLDGSCIYLKQ-QCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLD------EFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
S DF C + CIN++L CDG C+D+SDE Q C C NGRCIG CDGT C D +DE+ C ++ G F +G I T C+P +C T C C CDG DC D SDE CP K++C + F C RCI CD +DC + +DE C C +F+C + CI CDG NDCG + DE D+ + S + P P + + DC + C Y N C +C Q I ++ + C ++E P E C +++C +G C+ K +CD +DC+D DE P P C + + C +G C CD DC D SDE +C
Sbjct: 2550 SVDDFECGNGDCINYSLTCDGMAHCKDKSDEKQSYCANRLCKKGYRRCINGRCIGHMFWCDGTDDCGDHSDELPCN-MTLCKVGEFQCRDGSCISNTSRCDQVVNCEDASDEMNCLPTDCSRYFRLGVKGATFKSCERTTLCYLPSWQCDGNNDCGDYSDE--RNCPDKKKLKCPVNF-FAC-PSGRCIPMSWTCDKENDCENGADETHCDKF-CTSTEFECG-NHRCISNHWVCDGANDCGDNSDE---DSRCKTKTCSPEAFQCPGTHVCVPQRWKCDGDKDCLDGADESVKAGC-----LYNNTCDENEFTCQDRQCIPKHFVCDHDVDCSDGSDESPQCEYPTCGPEEFRCNNGRCLNQKSWECDGDFDCQDRSDEA----------PKNPRCTDSEGKCNDSAYMCHNGKCLNETLLCDRNDDCGDGSDELNC 2962 HSP 6 Score: 96.6709 bits (239), Expect = 5.669e-17 Identity = 113/380 (29.74%), Postives = 151/380 (39.74%), Query Frame = 0
Query: 79 YQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN------DCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCE--TEEDPVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGS-CRPIDTRCDGMVDCEDESDEFDC 444
Y PP C +F+CNN RCI + +CDG C D++DE P C + T +++C N+R CI R LCDG DC DE C C Q+ C RCI CD DC D SDE CF + QF C +G CI+ RCD NDCG + DE C + + F N S +PE +C D + Y + T + P GC V+++QC +DG CI ++ +CD DC D DE C E + + CDP +F+C++ + C CDG DCED SDE +C
Sbjct: 874 YIPPPQCQLG-EFSCNNNRCIQERWKCDGDNDCLDNSDE-----------------------------APELCHQHTCPSDRFKCQNNR-CIPLRWLCDGDNDCGTYEDESNTTCSARTCPPN-QYACA-SGRCIPTSWTCDLDDDCGDRSDEPASCAYPTCFPLT----QFTC-ANGRCINVNWRCDNDNDCGDNSDEAGCSHSCSSVQFKCN-------------SGRCIPE-----------------------------YWTCDGDNDCGDYSDETHANCTNQATRPPGGCHVDEFQCRMDGLCIPMRWRCDGDTDCMDLSDESDC--EGVTHM-----CDPAVKFSCRNSARCISKAWVCDGDSDCEDNSDEDNC 1166 HSP 7 Score: 93.5893 bits (231), Expect = 4.845e-16 Identity = 111/388 (28.61%), Postives = 149/388 (38.40%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPS-CDPNTQFTCNNGR---------CIGQDLRCDGTPQCQDSTDEIGCGAISKVA--PGFFSSLNGRIIGLTF-----DPCVPG----ECDE---ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEND----CFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENE--CRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQ------GCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIE 398
C +F C D +CI+ RCD +CED SDE C P+ C + C +CDG C D +DE C K+ FF+ +GR I +++ + C G CD+ T++EC NH RCI +CDG DC D SDED+ C C E FQC C+ +R +CDG DCLD +DE+ + CD +F C+ CI CD DC DE P P CG + N K+ C D + Q ++E P P+ C + + C +G C+ CDR DC DG DE C I
Sbjct: 2629 CKVGEFQCRDGSCISNTSRCDQVVNCEDASDEMNCLPTDCSRYFRLGVKGATFKSCERTTLCYLPSWQCDGNNDCGDYSDERNCPDKKKLKCPVNFFACPSGRCIPMSWTCDKENDCENGADETHCDKFCTSTEFECGNH-RCISNHWVCDGANDCGDNSDEDSR-CKTKTCSPE-AFQCPGTHVCVPQRWKCDGDKDCLDGADESVKAGCLYNNTCDENEFTCQ-DRQCIPKHFVCDHDVDCSDGSDES-----------------------------------------PQCEYPTCGPEEFRCNNGRCLNQKSWECDGDFDCQD------RSDEAPKNPRCTDSEGKCNDSAYMCHNGKCLNETLLCDRNDDCGDGSDELNCFIN 2965 HSP 8 Score: 93.2041 bits (230), Expect = 6.236e-16 Identity = 110/376 (29.26%), Postives = 148/376 (39.36%), Query Frame = 0
Query: 84 SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYW--------QFK-CRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYE-------GPCETEEDPVEPQGCAVNQWQCLDGS-CIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDE 441
SC F C NG CI L CDG C+D +DE +++ G+ +NGR IG F CDG DC D SDE + GE FQCRD CI RCD +C D SDE +C +C + FK C + LC +CDG NDCG DE++C + + N P + P+S E+DC G D + C C + + I ++ G E+ + + C+ +QC C+ + +CD DC DG DE + + CD +EFTC+D C P CD VDC D SDE
Sbjct: 2547 SCGSVDDFECGNGDCINYSLTCDGMAHCKDKSDEKQSYCANRLCKKGYRRCINGRCIGHMF---------------------------WCDGTDDCGDHSDELPCNMTLCKVGE---FQCRDGS-CISNTSRCDQVVNCEDASDEMNCLPTDCSRYFRLGVKGATFKSCERTTLCYLPSWQCDGNNDCGDYSDERNCPD-KKKLKCPVNFFACPSGRCI-PMSWTCDKENDCEN----------GADETHCDKFCTSTEFECGNHRCISNHWVCDGANDCGDNSDEDSRCKTKTCSPEAFQCPGTHVCVPQRWKCDGDKDCLDGADESVKAGCLYNN-----TCDE-NEFTCQDRQCIPKHFVCDHDVDCSDGSDE 2873 HSP 9 Score: 90.5077 bits (223), Expect = 4.375e-15 Identity = 80/250 (32.00%), Postives = 117/250 (46.80%), Query Frame = 0
Query: 38 PQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDEETQ----------WECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC----FAVECDYWQFKCRISGLCIDTRRRCDGTNDC---GSDE 265
P+++ E C F C++ C+ +CD DC D SDE +C P ++F C NGRCI +CDG C D +DE GC + F NG I + C+ G +E+ ++C N+ C CDG DC D SDE+ + C +C F+C++ + C+ ++CDG +C D SDE +C C+ +F C +G CI + RC+ NDC GSDE
Sbjct: 3550 PKEECDERTCEPYQFRCKNNRCVPGRWQCDYDNDCGDNSDEDKCVPRPCSESEFACTNGRCIAGRWKCDGDHDCADGSDEDGCEL--RCDSDQFQCKNGHCIPSRWRCDADADCLDGSDEEKCHIAPKHCPLDDFQC-NNTLCKPLAWKCDGEDDCGDNSDENPDQCRMFQCPPTRTFRCQNDRVCLQVSKQCDGVDNCGDNSDELNCQPPPAEPTCEKDEFLC-ANGGCISSSLRCNFFNDCEDYGSDE 3795 HSP 10 Score: 75.8702 bits (185), Expect = 2.038e-10 Identity = 72/230 (31.30%), Postives = 99/230 (43.04%), Query Frame = 0
Query: 41 DIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEM-CPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN-DCFAVECDYWQFKCRISGLCIDTRR-RCDGTNDCGSDEDE 267
D + +C F C CI + CD + DCE+ +DE C C +T+F C N RCI CDG C D++DE +P F +C C+ +R CDG DC D +DE + C +E +F C+D+Q CI K CD DC D SDE+ C C +F+C +G C++ + CDG DC DE
Sbjct: 2714 DKKKLKCPVNFFACPSGRCIPMSWTCDKENDCENGADETHCDKFC-TSTEFECGNHRCISNHWVCDGANDCGDNSDEDSRCKTKTCSPEAF--------------------------QCPGTHVCVPQRWKCDGDKDCLDGADESVKAGCLYNNTCDENEFTCQDRQ-CIPKHFVCDHDVDCSDGSDESPQCEYPTCGPEEFRCN-NGRCLNQKSWECDGDFDCQDRSDE 2914
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. nr
Match: gi|929229703|ref|XP_013991369.1| (PREDICTED: low-density lipoprotein receptor-related protein 1-like isoform X1 [Salmo salar]) HSP 1 Score: 154.836 bits (390), Expect = 8.724e-36 Identity = 143/425 (33.65%), Postives = 199/425 (46.82%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDE-YQCPP-SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDEE--------TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE--NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDE-------FDCP 445
+ C+ + F+C++ CI F +CD + DC DRSDE +CP C P QF C G C CDG CQD++DE C + F S R I F D C GE +++ TQ++CA +RCI + +CD DC D SDE A C ++ CG + +F+C+D RCI R +CDG +DC D SDE +C C+ +QF+C+ + C+ R +CD NDCG + DE C VP RP ++ C C D + +G ++ED E + C +Q+QC +G CI + +CD DC DG DE+ C I AP +C PLD+F C + C+P+ +CDG DC D SDE F CP
Sbjct: 3335 SNCTASQFVCKNDKCIPFWWKCDTEDDCGDRSDEPAECPEFKCRPG-QFQCGTGICTNPAYICDGDNDCQDNSDEANCDIHVCLPSQFKCSHPSRCIPGIFRCNGQDNCGEGEDEKDCPEVTCAPTQFQCAITKRCIPRVWVCDRDNDCVDGSDEPAN-CTQMTCGVD-EFRCKDSGRCIPARWKCDGENDCGDSSDEPKEECDERTCEPYQFRCK-NNRCVPGRWQCDYDNDCGDNSDEDKC-----------------VP------------------------RPCSESEFACTNGRCIAGRWKCDGDHDCA---DG---SDEDGCELR-CDSDQFQCKNGHCIPNRWRCDADADCLDGSDEEKCHI-------APKHC-PLDDFQCNNTLCKPLAWKCDGEDDCGDNSDENPDECRRFQCP 3699 HSP 2 Score: 110.923 bits (276), Expect = 1.743e-21 Identity = 122/410 (29.76%), Postives = 161/410 (39.27%), Query Frame = 0
Query: 42 IPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEY--QCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFD---------------PCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDED--AEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--------CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCE--TEEDPVEPQGCAVNQWQCL--DGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCD--PLDEFTC 417
+P QC +F C++ CI +CDG DC D SDE C P+ +F C N RCI CDG C DE ++ P ++ +GR I ++ C C TQ+ CAN R CI+ CD DC D SDE + C V QF+C + RCI + CDG +DC D SDE C +F+CR+ GLCI R RCDG DC DE DC+ +T C + C C KA C D + CE ++ED E C ++ C+ D C+ ++ CD DC DG DEK C + +ID NC P + F C
Sbjct: 855 VPPPQCQLGEFACKNNRCIQKRWKCDGDNDCLDNSDEAPELCHQHTCPSDRFKCQNNRCIPLRWLCDGDNDCGTDEDESNSTCSARTCPPNQYACASGRCIPTSWTCDLDDDCGDRSDEPASCAYPTCFPLTQFTCANGR-CINVNWRCDNDNDCGDNSDEAGCSHSCSSV------QFKC-NSGRCIPEYWTCDGDNDCGDYSDETHANCTNQATRPPGGCHVDEFQCRMDGLCIPMRWRCDGDADCMDLSDESDCEGVTH--------------------------------MCDPAVKFSCRNSA-----RCISKAWVCDGDSD--------CEDNSDEDNCEALVCKLSHHVCVTNDSICLPAEKLCDGTDDCPDGSDEKLCDLCSIDNGGCSHNCSIIPGEGFMC 1211 HSP 3 Score: 103.219 bits (256), Expect = 4.707e-19 Identity = 137/423 (32.39%), Postives = 173/423 (40.90%), Query Frame = 0
Query: 39 QDDIPEAQCSRTDFICEDLA-CINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC--------NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITEN-PF--FSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDE-SDEFDC 444
+ D PE C+ T F C CI CD DC D SDE P +C TQ TC ++GRCI +CDG C DS+DE ECDE T Q+ C N+ RC+ R CD DC D SDED C C E +F C + RCI R +CDG DC D SDE+ C + CD QF+C+ +G CI R RCD DC DE+ C ++ P F N T P++ + EDD CG + +ECR ++ P P C D C+ + +QCD +C D DE C PA P+C+ DEF C +G C RC+ DCED SDE C
Sbjct: 3448 EKDCPEVTCAPTQFQCAITKRCIPRVWVCDRDNDCVDGSDE---PANC---TQMTCGVDEFRCKDSGRCIPARWKCDGENDCGDSSDE-----------------------------PKEECDERTCEPYQFRCKNN-RCVPGRWQCDYDNDCGDNSDEDK--CVPRPCSES-EFACTNG-RCIAGRWKCDGDHDCADGSDEDGC-ELRCDSDQFQCK-NGHCIPNRWRCDADADCLDGSDEEKCHIAPKHCPLDDFQCNN------TLCKPLAWKCDGEDD------------CGDNSDENPDECR--------------RFQCP------PTRTFRCQN------DRVCLQVSKQCDGVDNCGDNSDELNCQ-----PPPAEPSCEK-DEFLCANGRCISSSLRCNFFNDCEDYGSDEIHC 3778 HSP 4 Score: 97.0561 bits (240), Expect = 4.636e-17 Identity = 114/382 (29.84%), Postives = 158/382 (41.36%), Query Frame = 0
Query: 115 TDEIGCGAISKVAPG----------FFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPF-FSTNEIEEPVPTT-VSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTY-------------------------------ENECRLKAMSCSSDQ----EIQIYYEGPC--ETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLG 447
TD GC + ++PG F+ + +GR C +Q+ C N + CI CD DC D+SDE AE CP+ +C QFQC C + CDG +DC D SDE +C C QFKC CI RC+G ++CG EDE+DC +T P F + +P V + + D P C M+ CG D E C C +++ Q Y+ C ++ED P+ C+ +++ C +G CI + +CD +DC DG DE C + CD D+F CK+G C P RCD DC D SDE C +
Sbjct: 3299 TDNGGCSNLCLLSPGGGYKCACPTNFYLANDGRT---CMSNCTA------SQFVCKNDK-CIPFWWKCDTEDDCGDRSDEPAE-CPEFKC-RPGQFQC-GTGICTNPAYICDGDNDCQDNSDEANCDIHVCLPSQFKCSHPSRCIPGIFRCNGQDNCGEGEDEKDCPEVTCAPTQFQCAITKRCIPRVWVCDRDNDCVDGSDEPANCTQMT---CGVDEFRCKDSGRCIPARWKCDGENDCGDSSDEPKEECDERTCEPYQFRCKNNRCVPGRWQCDYDNDCGDNSDEDKCVPRPCSESEFACTNGRCIAGRWKCDGDHDCADGSDEDGCELR----------CDS-DQFQCKNGHCIPNRWRCDADADCLDGSDEEKCHIA 3653 HSP 5 Score: 94.3597 bits (233), Expect = 3.052e-16 Identity = 126/438 (28.77%), Postives = 174/438 (39.73%), Query Frame = 0
Query: 48 SRTDFICEDLACINFALRCDGKFDCEDRSDEYQ--CPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFD--------------PCVPGECDE--------ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCP---KVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYY----EGPCE--TEEDP-VEPQGCAVNQWQCLDGSCIYLKQ-QCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDE------FTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
S DF C + CIN++L CDG C+D+SDE Q C C NGRCIG CDGT C D +DE+ C ++ G F +G I T C+P +C T C C CDG DC D SDE CP K++C + F C RCI CD +DC + +DE C C +F+C + CI CDG +DCG + DE D+ + S + P P + + DC + C Y N C C Q + ++ + C ++E P E C +++C +G C+ K +CD +DC+D DE P P C + + C++G C CD DC D SDE +C
Sbjct: 2530 SVDDFECGNGDCINYSLTCDGMAHCKDKSDEKQSYCANRLCKKGYRRCINGRCIGHIFWCDGTDDCGDHSDELPCN-MTLCKAGEFQCRDGSCISNTSRCDQVVNCEDASDEMNCLPTDCSRYFRLGVKGATFQSCERTTLCYLPSWQCDGNNDCGDFSDE--RNCPDKKKLKCPVNF-FAC-PSGRCIPMSWTCDKENDCENGADETHCDKF-CTSTEFECG-NHRCISNHWVCDGADDCGDNSDE---DSRCKTKTCSPEAFQCPGTHVCVPQRWKCDGDKDCLDGADESVKAGC-----LYNNTCDENEFMCQDRQCVPKHFVCDHDIDCSDGSDESPQCEYPTCGPEEFRCNNGRCLNQKSWECDGDFDCQDRSDEA----------PKNPRCTDSERKCNDSAYMCRNGKCLNETLLCDRNDDCGDGSDELNC 2942 HSP 6 Score: 92.8189 bits (229), Expect = 9.304e-16 Identity = 110/388 (28.35%), Postives = 149/388 (38.40%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPS-CDPNTQFTCNNGR---------CIGQDLRCDGTPQCQDSTDEIGCGAISKVA--PGFFSSLNGRIIGLTF-----DPCVPG----ECDE---ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEND----CFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENE--CRLKAMSCSSDQEIQIYYEGPCETEEDPVEP------QGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIE 398
C +F C D +CI+ RCD +CED SDE C P+ C + C +CDG C D +DE C K+ FF+ +GR I +++ + C G CD+ T++EC NH RCI +CDG DC D SDED+ C C E FQC C+ +R +CDG DCLD +DE+ + CD +F C+ C+ CD DC DE P P CG + N K+ C D + Q ++E P P + C + + C +G C+ CDR DC DG DE C I
Sbjct: 2609 CKAGEFQCRDGSCISNTSRCDQVVNCEDASDEMNCLPTDCSRYFRLGVKGATFQSCERTTLCYLPSWQCDGNNDCGDFSDERNCPDKKKLKCPVNFFACPSGRCIPMSWTCDKENDCENGADETHCDKFCTSTEFECGNH-RCISNHWVCDGADDCGDNSDEDSR-CKTKTCSPE-AFQCPGTHVCVPQRWKCDGDKDCLDGADESVKAGCLYNNTCDENEFMCQ-DRQCVPKHFVCDHDIDCSDGSDES-----------------------------------------PQCEYPTCGPEEFRCNNGRCLNQKSWECDGDFDCQD------RSDEAPKNPRCTDSERKCNDSAYMCRNGKCLNETLLCDRNDDCGDGSDELNCFIN 2945 HSP 7 Score: 91.6633 bits (226), Expect = 2.145e-15 Identity = 80/250 (32.00%), Postives = 118/250 (47.20%), Query Frame = 0
Query: 38 PQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDEETQ----------WECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC----FAVECDYWQFKCRISGLCIDTRRRCDGTNDC---GSDE 265
P+++ E C F C++ C+ +CD DC D SDE +C P ++F C NGRCI +CDG C D +DE GC + F NG I + C+ G +E+ ++C N+ C CDG DC D SDE+ + C + +C F+C++ + C+ ++CDG +C D SDE +C C+ +F C +G CI + RC+ NDC GSDE
Sbjct: 3530 PKEECDERTCEPYQFRCKNNRCVPGRWQCDYDNDCGDNSDEDKCVPRPCSESEFACTNGRCIAGRWKCDGDHDCADGSDEDGCEL--RCDSDQFQCKNGHCIPNRWRCDADADCLDGSDEEKCHIAPKHCPLDDFQC-NNTLCKPLAWKCDGEDDCGDNSDENPDECRRFQCPPTRTFRCQNDRVCLQVSKQCDGVDNCGDNSDELNCQPPPAEPSCEKDEFLC-ANGRCISSSLRCNFFNDCEDYGSDE 3775 HSP 8 Score: 90.8929 bits (224), Expect = 4.116e-15 Identity = 107/376 (28.46%), Postives = 147/376 (39.10%), Query Frame = 0
Query: 84 SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECD-YWQF--------KCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYE-------GPCETEEDPVEPQGCAVNQWQCLDGS-CIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDE 441
SC F C NG CI L CDG C+D +DE +++ G+ +NGR IG F CDG DC D SDE + GE FQCRD CI RCD +C D SDE +C +C Y++ C + LC +CDG NDCG DE++C + + N P + P+S E+DC G D + C C + + I ++ G E+ + + C+ +QC C+ + +CD DC DG DE + + CD +EF C+D C P CD +DC D SDE
Sbjct: 2527 SCGSVDDFECGNGDCINYSLTCDGMAHCKDKSDEKQSYCANRLCKKGYRRCINGRCIGHIF---------------------------WCDGTDDCGDHSDELPCNMTLCKAGE---FQCRDGS-CISNTSRCDQVVNCEDASDEMNCLPTDCSRYFRLGVKGATFQSCERTTLCYLPSWQCDGNNDCGDFSDERNCPD-KKKLKCPVNFFACPSGRCI-PMSWTCDKENDCEN----------GADETHCDKFCTSTEFECGNHRCISNHWVCDGADDCGDNSDEDSRCKTKTCSPEAFQCPGTHVCVPQRWKCDGDKDCLDGADESVKAGCLYNN-----TCDE-NEFMCQDRQCVPKHFVCDHDIDCSDGSDE 2853 HSP 9 Score: 86.6557 bits (213), Expect = 8.421e-14 Identity = 91/278 (32.73%), Postives = 125/278 (44.96%), Query Frame = 0
Query: 190 PKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEND--CFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLP-------EDDC------PTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVE-------PQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCK-DGSCRPIDTRCDGMVDCEDESDEFDC 444
P+ + GE F C++ RCI KR +CDG +DCLD SDE C C +FKC+ + CI R CDG NDCG+DEDE + ST P + S +P +DDC P C + P C L +C++ + I + + C+ + D + C+ Q++C G CI CD DC D DE + + ++ PP +DEF C+ DG C P+ RCDG DC D SDE DC
Sbjct: 858 PQCQLGE---FACKN-NRCIQKRWKCDGDNDCLDNSDEAPELCHQHTCPSDRFKCQ-NNRCIPLRWLCDGDNDCGTDEDESN----------STCSARTCPPNQYACASGRCIPTSWTCDLDDDCGDRSDEPASC---AYPTCFP----------LTQFTCANGRCINVNWR--CDNDNDCGDNSDEAGCSHSCSSVQFKCNSGRCIPEYWTCDGDNDCGDYSDETHAN--CTNQATRPPGGCHVDEFQCRMDGLCIPMRWRCDGDADCMDLSDESDC 1103 HSP 10 Score: 81.2629 bits (199), Expect = 3.842e-12 Identity = 114/423 (26.95%), Postives = 149/423 (35.22%), Query Frame = 0
Query: 79 YQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN------DCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCE--TEEDPVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCK------------------------------------------DGSCRPIDTRCDGMVDCEDESDEFDCPL 446
Y PP C +F C N RCI + +CDG C D++DE P C + T +++C N+R CI R LCDG DC DE C C Q+ C RCI CD DC D SDE CF + QF C +G CI+ RCD NDCG + DE C + + F N S +PE +C D + Y + T + P GC V+++QC +DG CI ++ +CD DC D DE C E + + CDP +F+C+ D C P + CDG DC D SDE C L
Sbjct: 854 YVPPPQCQLG-EFACKNNRCIQKRWKCDGDNDCLDNSDE-----------------------------APELCHQHTCPSDRFKCQNNR-CIPLRWLCDGDNDCGTDEDESNSTCSARTCPPN-QYACA-SGRCIPTSWTCDLDDDCGDRSDEPASCAYPTCFPLT----QFTC-ANGRCINVNWRCDNDNDCGDNSDEAGCSHSCSSVQFKCN-------------SGRCIPE-----------------------------YWTCDGDNDCGDYSDETHANCTNQATRPPGGCHVDEFQCRMDGLCIPMRWRCDGDADCMDLSDESDC--EGVTHM-----CDPAVKFSCRNSARCISKAWVCDGDSDCEDNSDEDNCEALVCKLSHHVCVTNDSICLPAEKLCDGTDDCPDGSDEKLCDL 1189 HSP 11 Score: 76.2554 bits (186), Expect = 1.457e-10 Identity = 81/262 (30.92%), Postives = 116/262 (44.27%), Query Frame = 0
Query: 41 DIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVP--GECD-----------------------EETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRR-RCDGYSDCLDESDEN----DCFAVE--CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
D + +C F C CI + CD + DCE+ +DE C C +T+F C N RCI CDG C D++DE +P F + CVP +CD +E ++ C + R+C+ K +CD DC D SDE + C CG E +F+C + RC++++ CDG DC D SDE C E C+ + CR +G C++ CD +DCG DE +C
Sbjct: 2694 DKKKLKCPVNFFACPSGRCIPMSWTCDKENDCENGADETHCDKFC-TSTEFECGNHRCISNHWVCDGADDCGDNSDEDSRCKTKTCSPEAFQCPGTHV-------CVPQRWKCDGDKDCLDGADESVKAGCLYNNTCDENEFMCQD-RQCVPKHFVCDHDIDCSDGSDESPQ-CEYPTCGPE-EFRC-NNGRCLNQKSWECDGDFDCQDRSDEAPKNPRCTDSERKCNDSAYMCR-NGKCLNETLLCDRNDDCGDGSDELNC 2942
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. nr
Match: gi|929229705|ref|XP_013991370.1| (PREDICTED: low-density lipoprotein receptor-related protein 1-like isoform X2 [Salmo salar]) HSP 1 Score: 154.836 bits (390), Expect = 9.387e-36 Identity = 143/425 (33.65%), Postives = 199/425 (46.82%), Query Frame = 0
Query: 45 AQCSRTDFICEDLACINFALRCDGKFDCEDRSDE-YQCPP-SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDEE--------TQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE--NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDE-------FDCP 445
+ C+ + F+C++ CI F +CD + DC DRSDE +CP C P QF C G C CDG CQD++DE C + F S R I F D C GE +++ TQ++CA +RCI + +CD DC D SDE A C ++ CG + +F+C+D RCI R +CDG +DC D SDE +C C+ +QF+C+ + C+ R +CD NDCG + DE C VP RP ++ C C D + +G ++ED E + C +Q+QC +G CI + +CD DC DG DE+ C I AP +C PLD+F C + C+P+ +CDG DC D SDE F CP
Sbjct: 3333 SNCTASQFVCKNDKCIPFWWKCDTEDDCGDRSDEPAECPEFKCRPG-QFQCGTGICTNPAYICDGDNDCQDNSDEANCDIHVCLPSQFKCSHPSRCIPGIFRCNGQDNCGEGEDEKDCPEVTCAPTQFQCAITKRCIPRVWVCDRDNDCVDGSDEPAN-CTQMTCGVD-EFRCKDSGRCIPARWKCDGENDCGDSSDEPKEECDERTCEPYQFRCK-NNRCVPGRWQCDYDNDCGDNSDEDKC-----------------VP------------------------RPCSESEFACTNGRCIAGRWKCDGDHDCA---DG---SDEDGCELR-CDSDQFQCKNGHCIPNRWRCDADADCLDGSDEEKCHI-------APKHC-PLDDFQCNNTLCKPLAWKCDGEDDCGDNSDENPDECRRFQCP 3697 HSP 2 Score: 110.923 bits (276), Expect = 1.805e-21 Identity = 122/410 (29.76%), Postives = 161/410 (39.27%), Query Frame = 0
Query: 42 IPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEY--QCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFD---------------PCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDED--AEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--------CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCE--TEEDPVEPQGCAVNQWQCL--DGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCD--PLDEFTC 417
+P QC +F C++ CI +CDG DC D SDE C P+ +F C N RCI CDG C DE ++ P ++ +GR I ++ C C TQ+ CAN R CI+ CD DC D SDE + C V QF+C + RCI + CDG +DC D SDE C +F+CR+ GLCI R RCDG DC DE DC+ +T C + C C KA C D + CE ++ED E C ++ C+ D C+ ++ CD DC DG DEK C + +ID NC P + F C
Sbjct: 855 VPPPQCQLGEFACKNNRCIQKRWKCDGDNDCLDNSDEAPELCHQHTCPSDRFKCQNNRCIPLRWLCDGDNDCGTDEDESNSTCSARTCPPNQYACASGRCIPTSWTCDLDDDCGDRSDEPASCAYPTCFPLTQFTCANGR-CINVNWRCDNDNDCGDNSDEAGCSHSCSSV------QFKC-NSGRCIPEYWTCDGDNDCGDYSDETHANCTNQATRPPGGCHVDEFQCRMDGLCIPMRWRCDGDADCMDLSDESDCEGVTH--------------------------------MCDPAVKFSCRNSA-----RCISKAWVCDGDSD--------CEDNSDEDNCEALVCKLSHHVCVTNDSICLPAEKLCDGTDDCPDGSDEKLCDLCSIDNGGCSHNCSIIPGEGFMC 1211 HSP 3 Score: 103.219 bits (256), Expect = 4.832e-19 Identity = 137/423 (32.39%), Postives = 173/423 (40.90%), Query Frame = 0
Query: 39 QDDIPEAQCSRTDFICEDLA-CINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC--------NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITEN-PF--FSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDE-SDEFDC 444
+ D PE C+ T F C CI CD DC D SDE P +C TQ TC ++GRCI +CDG C DS+DE ECDE T Q+ C N+ RC+ R CD DC D SDED C C E +F C + RCI R +CDG DC D SDE+ C + CD QF+C+ +G CI R RCD DC DE+ C ++ P F N T P++ + EDD CG + +ECR ++ P P C D C+ + +QCD +C D DE C PA P+C+ DEF C +G C RC+ DCED SDE C
Sbjct: 3446 EKDCPEVTCAPTQFQCAITKRCIPRVWVCDRDNDCVDGSDE---PANC---TQMTCGVDEFRCKDSGRCIPARWKCDGENDCGDSSDE-----------------------------PKEECDERTCEPYQFRCKNN-RCVPGRWQCDYDNDCGDNSDEDK--CVPRPCSES-EFACTNG-RCIAGRWKCDGDHDCADGSDEDGC-ELRCDSDQFQCK-NGHCIPNRWRCDADADCLDGSDEEKCHIAPKHCPLDDFQCNN------TLCKPLAWKCDGEDD------------CGDNSDENPDECR--------------RFQCP------PTRTFRCQN------DRVCLQVSKQCDGVDNCGDNSDELNCQ-----PPPAEPSCEK-DEFLCANGRCISSSLRCNFFNDCEDYGSDEIHC 3776 HSP 4 Score: 96.6709 bits (239), Expect = 4.971e-17 Identity = 117/383 (30.55%), Postives = 160/383 (41.78%), Query Frame = 0
Query: 115 TDEIGCGAISKVAPG----------FFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPF-FSTNEIEEPVPTT-VSPISIEVLPEDDCPTFCPSMSRPVCGTDGLT---------------YENEC---------------------RLKAMSCSSDQEIQIYYEGPC--ETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLG 447
TD GC + ++PG F+ + +GR C +Q+ C N + CI CD DC D+SDE AE CP+ +C QFQC C + CDG +DC D SDE +C C QFKC CI RC+G ++CG EDE+DC +T P F + +P V + + D P C M+ CG D EN+C R K C + Q Y+ C ++ED P+ C+ +++ C +G CI + +CD +DC DG DE C + CD D+F CK+G C P RCD DC D SDE C +
Sbjct: 3297 TDNGGCSNLCLLSPGGGYKCACPTNFYLANDGRT---CMSNCTA------SQFVCKNDK-CIPFWWKCDTEDDCGDRSDEPAE-CPEFKC-RPGQFQC-GTGICTNPAYICDGDNDCQDNSDEANCDIHVCLPSQFKCSHPSRCIPGIFRCNGQDNCGEGEDEKDCPEVTCAPTQFQCAITKRCIPRVWVCDRDNDCVDGSDEPANCTQMT---CGVDEFRCKDSGRCIPARWKCDGENDCGDSSDEPKEECDERTCEPYQFRCKNNRCVPGR-WQCDYDNDCGDNSDEDKCVPRPCSESEFACTNGRCIAGRWKCDGDHDCADGSDEDGCELR----------CDS-DQFQCKNGHCIPNRWRCDADADCLDGSDEEKCHIA 3651 HSP 5 Score: 94.3597 bits (233), Expect = 3.188e-16 Identity = 126/438 (28.77%), Postives = 174/438 (39.73%), Query Frame = 0
Query: 48 SRTDFICEDLACINFALRCDGKFDCEDRSDEYQ--CPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFD--------------PCVPGECDE--------ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCP---KVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYY----EGPCE--TEEDP-VEPQGCAVNQWQCLDGSCIYLKQ-QCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDE------FTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
S DF C + CIN++L CDG C+D+SDE Q C C NGRCIG CDGT C D +DE+ C ++ G F +G I T C+P +C T C C CDG DC D SDE CP K++C + F C RCI CD +DC + +DE C C +F+C + CI CDG +DCG + DE D+ + S + P P + + DC + C Y N C C Q + ++ + C ++E P E C +++C +G C+ K +CD +DC+D DE P P C + + C++G C CD DC D SDE +C
Sbjct: 2528 SVDDFECGNGDCINYSLTCDGMAHCKDKSDEKQSYCANRLCKKGYRRCINGRCIGHIFWCDGTDDCGDHSDELPCN-MTLCKAGEFQCRDGSCISNTSRCDQVVNCEDASDEMNCLPTDCSRYFRLGVKGATFQSCERTTLCYLPSWQCDGNNDCGDFSDE--RNCPDKKKLKCPVNF-FAC-PSGRCIPMSWTCDKENDCENGADETHCDKF-CTSTEFECG-NHRCISNHWVCDGADDCGDNSDE---DSRCKTKTCSPEAFQCPGTHVCVPQRWKCDGDKDCLDGADESVKAGC-----LYNNTCDENEFMCQDRQCVPKHFVCDHDIDCSDGSDESPQCEYPTCGPEEFRCNNGRCLNQKSWECDGDFDCQDRSDEA----------PKNPRCTDSERKCNDSAYMCRNGKCLNETLLCDRNDDCGDGSDELNC 2940 HSP 6 Score: 92.8189 bits (229), Expect = 9.717e-16 Identity = 110/388 (28.35%), Postives = 149/388 (38.40%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPS-CDPNTQFTCNNGR---------CIGQDLRCDGTPQCQDSTDEIGCGAISKVA--PGFFSSLNGRIIGLTF-----DPCVPG----ECDE---ETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEND----CFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENE--CRLKAMSCSSDQEIQIYYEGPCETEEDPVEP------QGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIE 398
C +F C D +CI+ RCD +CED SDE C P+ C + C +CDG C D +DE C K+ FF+ +GR I +++ + C G CD+ T++EC NH RCI +CDG DC D SDED+ C C E FQC C+ +R +CDG DCLD +DE+ + CD +F C+ C+ CD DC DE P P CG + N K+ C D + Q ++E P P + C + + C +G C+ CDR DC DG DE C I
Sbjct: 2607 CKAGEFQCRDGSCISNTSRCDQVVNCEDASDEMNCLPTDCSRYFRLGVKGATFQSCERTTLCYLPSWQCDGNNDCGDFSDERNCPDKKKLKCPVNFFACPSGRCIPMSWTCDKENDCENGADETHCDKFCTSTEFECGNH-RCISNHWVCDGADDCGDNSDEDSR-CKTKTCSPE-AFQCPGTHVCVPQRWKCDGDKDCLDGADESVKAGCLYNNTCDENEFMCQ-DRQCVPKHFVCDHDIDCSDGSDES-----------------------------------------PQCEYPTCGPEEFRCNNGRCLNQKSWECDGDFDCQD------RSDEAPKNPRCTDSERKCNDSAYMCRNGKCLNETLLCDRNDDCGDGSDELNCFIN 2943 HSP 7 Score: 91.6633 bits (226), Expect = 2.201e-15 Identity = 80/250 (32.00%), Postives = 118/250 (47.20%), Query Frame = 0
Query: 38 PQDDIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDEETQ----------WECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC----FAVECDYWQFKCRISGLCIDTRRRCDGTNDC---GSDE 265
P+++ E C F C++ C+ +CD DC D SDE +C P ++F C NGRCI +CDG C D +DE GC + F NG I + C+ G +E+ ++C N+ C CDG DC D SDE+ + C + +C F+C++ + C+ ++CDG +C D SDE +C C+ +F C +G CI + RC+ NDC GSDE
Sbjct: 3528 PKEECDERTCEPYQFRCKNNRCVPGRWQCDYDNDCGDNSDEDKCVPRPCSESEFACTNGRCIAGRWKCDGDHDCADGSDEDGCEL--RCDSDQFQCKNGHCIPNRWRCDADADCLDGSDEEKCHIAPKHCPLDDFQC-NNTLCKPLAWKCDGEDDCGDNSDENPDECRRFQCPPTRTFRCQNDRVCLQVSKQCDGVDNCGDNSDELNCQPPPAEPSCEKDEFLC-ANGRCISSSLRCNFFNDCEDYGSDE 3773 HSP 8 Score: 90.8929 bits (224), Expect = 4.151e-15 Identity = 107/376 (28.46%), Postives = 147/376 (39.10%), Query Frame = 0
Query: 84 SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKV-APGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECD-YWQF--------KCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYE-------GPCETEEDPVEPQGCAVNQWQCLDGS-CIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDE 441
SC F C NG CI L CDG C+D +DE +++ G+ +NGR IG F CDG DC D SDE + GE FQCRD CI RCD +C D SDE +C +C Y++ C + LC +CDG NDCG DE++C + + N P + P+S E+DC G D + C C + + I ++ G E+ + + C+ +QC C+ + +CD DC DG DE + + CD +EF C+D C P CD +DC D SDE
Sbjct: 2525 SCGSVDDFECGNGDCINYSLTCDGMAHCKDKSDEKQSYCANRLCKKGYRRCINGRCIGHIF---------------------------WCDGTDDCGDHSDELPCNMTLCKAGE---FQCRDGS-CISNTSRCDQVVNCEDASDEMNCLPTDCSRYFRLGVKGATFQSCERTTLCYLPSWQCDGNNDCGDFSDERNCPD-KKKLKCPVNFFACPSGRCI-PMSWTCDKENDCEN----------GADETHCDKFCTSTEFECGNHRCISNHWVCDGADDCGDNSDEDSRCKTKTCSPEAFQCPGTHVCVPQRWKCDGDKDCLDGADESVKAGCLYNN-----TCDE-NEFMCQDRQCVPKHFVCDHDIDCSDGSDE 2851 HSP 9 Score: 86.6557 bits (213), Expect = 8.793e-14 Identity = 91/278 (32.73%), Postives = 125/278 (44.96%), Query Frame = 0
Query: 190 PKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEND--CFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLP-------EDDC------PTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVE-------PQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCK-DGSCRPIDTRCDGMVDCEDESDEFDC 444
P+ + GE F C++ RCI KR +CDG +DCLD SDE C C +FKC+ + CI R CDG NDCG+DEDE + ST P + S +P +DDC P C + P C L +C++ + I + + C+ + D + C+ Q++C G CI CD DC D DE + + ++ PP +DEF C+ DG C P+ RCDG DC D SDE DC
Sbjct: 858 PQCQLGE---FACKN-NRCIQKRWKCDGDNDCLDNSDEAPELCHQHTCPSDRFKCQ-NNRCIPLRWLCDGDNDCGTDEDESN----------STCSARTCPPNQYACASGRCIPTSWTCDLDDDCGDRSDEPASC---AYPTCFP----------LTQFTCANGRCINVNWR--CDNDNDCGDNSDEAGCSHSCSSVQFKCNSGRCIPEYWTCDGDNDCGDYSDETHAN--CTNQATRPPGGCHVDEFQCRMDGLCIPMRWRCDGDADCMDLSDESDC 1103 HSP 10 Score: 81.2629 bits (199), Expect = 4.116e-12 Identity = 114/423 (26.95%), Postives = 149/423 (35.22%), Query Frame = 0
Query: 79 YQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEET----QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEN------DCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCE--TEEDPVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCK------------------------------------------DGSCRPIDTRCDGMVDCEDESDEFDCPL 446
Y PP C +F C N RCI + +CDG C D++DE P C + T +++C N+R CI R LCDG DC DE C C Q+ C RCI CD DC D SDE CF + QF C +G CI+ RCD NDCG + DE C + + F N S +PE +C D + Y + T + P GC V+++QC +DG CI ++ +CD DC D DE C E + + CDP +F+C+ D C P + CDG DC D SDE C L
Sbjct: 854 YVPPPQCQLG-EFACKNNRCIQKRWKCDGDNDCLDNSDE-----------------------------APELCHQHTCPSDRFKCQNNR-CIPLRWLCDGDNDCGTDEDESNSTCSARTCPPN-QYACA-SGRCIPTSWTCDLDDDCGDRSDEPASCAYPTCFPLT----QFTC-ANGRCINVNWRCDNDNDCGDNSDEAGCSHSCSSVQFKCN-------------SGRCIPE-----------------------------YWTCDGDNDCGDYSDETHANCTNQATRPPGGCHVDEFQCRMDGLCIPMRWRCDGDADCMDLSDESDC--EGVTHM-----CDPAVKFSCRNSARCISKAWVCDGDSDCEDNSDEDNCEALVCKLSHHVCVTNDSICLPAEKLCDGTDDCPDGSDEKLCDL 1189 HSP 11 Score: 76.2554 bits (186), Expect = 1.444e-10 Identity = 81/262 (30.92%), Postives = 116/262 (44.27%), Query Frame = 0
Query: 41 DIPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVP--GECD-----------------------EETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRR-RCDGYSDCLDESDEN----DCFAVE--CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC 270
D + +C F C CI + CD + DCE+ +DE C C +T+F C N RCI CDG C D++DE +P F + CVP +CD +E ++ C + R+C+ K +CD DC D SDE + C CG E +F+C + RC++++ CDG DC D SDE C E C+ + CR +G C++ CD +DCG DE +C
Sbjct: 2692 DKKKLKCPVNFFACPSGRCIPMSWTCDKENDCENGADETHCDKFC-TSTEFECGNHRCISNHWVCDGADDCGDNSDEDSRCKTKTCSPEAFQCPGTHV-------CVPQRWKCDGDKDCLDGADESVKAGCLYNNTCDENEFMCQD-RQCVPKHFVCDHDIDCSDGSDESPQ-CEYPTCGPE-EFRC-NNGRCLNQKSWECDGDFDCQDRSDEAPKNPRCTDSERKCNDSAYMCR-NGKCLNETLLCDRNDDCGDGSDELNC 2940
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. nr
Match: gi|974066533|ref|XP_015230707.1| (PREDICTED: low-density lipoprotein receptor-related protein 2-like [Cyprinodon variegatus]) HSP 1 Score: 154.451 bits (389), Expect = 1.029e-35 Identity = 135/421 (32.07%), Postives = 191/421 (45.37%), Query Frame = 0
Query: 44 EAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNT----QFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVP--GECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVS----PISIEVLPEDDCPT------FCPSMSRPVCGTDGLTYENEC-RLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLG 447
+ +C+ F C++ CI + CDG DC D SDE P SC T +F C NGRC+ CD C D +DE +D C+ +CDE+T++ C + RC+ + CDG DC D SDE E C +V C F+C D RC+ R +CDG +DC D SDE DC C +F+C + CI RCD NDCG + DE+DC+ +T NP F + +P+ + P +++ E CPT +CP + T ++ C + SD+ + + CE +++C +G CIY C+ K DC DG DEK E + P P C L+EF C +G C + CD +C D +DE C G
Sbjct: 3575 DHRCAVNQFQCKNKNCIPVSWHCDGVNDCSDGSDED--PESCAQKTCQPGEFKCTNGRCVPMSYVCDAQNDCGDGSDE------------------------PYDTCMGPDYKCDEDTEFSCKTNYRCVPQWARCDGTNDCLDNSDE--EGCEEVTCDPLGDFRC-DNHRCVPIRWKCDGNNDCGDGSDEKDCEPRPCTESEFRCD-NAQCIPGIWRCDHQNDCGDNSDERDCELLTCNPGFFQCDSGHCIPSALQCDGRPDCLDLTDETTCPTRFPDGRWCPPLQFQCANTLCVSQSWVCDGVDDCGDRSDEVLSLCLNVTCEMP-----------TKFRCENGYCIYAGLMCNMKDDCGDGSDEK----EDLCREPTLPPC-TLEEFKCTNGHCVALPYVCDHNDNCGDRTDEMGCNFG 3949 HSP 2 Score: 136.346 bits (342), Expect = 9.184e-30 Identity = 132/422 (31.28%), Postives = 178/422 (42.18%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP-PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDST--DEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC---FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQ---DCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSR-PVCGTDGLTYENECRLKAMSCSSDQEIQIYYE----GPCETEEDPVE--PQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKP 452
CS DF C++ C+ + CD DC D SDE C P+C+P T+FTC+NGRCI D CDG C+D+T DEI C P Q +C + CI LCDG+ +C D SDE+ C C QFQC D+ +CI + CD +DC D++DE C QF C +G C+ CDG NDC + DE C + T + T P P++ E DC + P C + +C++ I I Y C D + C+ +Q+ C +G+CIY CD + DC DG DE +++ P P C P EF C G C + C+ DCED SDE C + P
Sbjct: 2724 CSSLDFTCDNGRCVPMSYVCDYTNDCGDNSDERDCAFPTCNPATEFTCSNGRCINSDFVCDGHNDCRDNTTSDEINC---------------------------PDRECPAGQIKCERNNICIYPENLCDGFNNCGDNSDENPLFCADRTCAAN-QFQC-DQGKCIPESWVCDSIADCNDKTDEPPSCEDIVRTCGPDQFTC-TNGNCVMQSVVCDGNNDCWDNSDEAPELQCGDRTCSTNQFTCPTWYPGHPRCLPLNFVCDGEKDCANAADELQNCP---------NRTCHMDEFACANGLCILIPYHCDRVNDCGDGSDELNCIYDTCSSSQFTCGNGACIYASYICDGENDCLDGSDEA----DSLCFTPQP-TCSP-QEFMCNSGECIDLHKVCNTQKDCEDNSDEKGCGINECLNP 3100 HSP 3 Score: 120.168 bits (300), Expect = 1.793e-24 Identity = 110/312 (35.26%), Postives = 146/312 (46.79%), Query Frame = 0
Query: 47 CSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP---PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGA-ISKVAPGFFSSLNGRIIGLTF-----DPCVPGECDEET--------QWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDE-NDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVL--PEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSD 338
CS F C++ CI + RCDG DC D SDE CP P+ + FTC+N +CI ++ CDG C D +DE CG+ IS PGFF + R + + C+ G DE+ ++ CA+ +CI CDG DC D SDE P + +FQC+ CI CDG+ DC D SDE N C AV C F+C + +CI T CDG NDC DEQ+C P FS + PT I ++ + + DCP + P+C D +C L CS D
Sbjct: 1061 CSPYAFTCDNKHCIYSSWRCDGFDDCGDGSDETDCPTRVPTTCSASYFTCDNDKCISKNWVCDGDNDCGDGSDEQNCGSTISSCPPGFFLCPDHRCLSSYYVCDGDQDCLDGS-DEKNCEFSCQSYEFACASGDQCISSYYRCDGVSDCRDHSDEQGCPTPGPGLCHDDEFQCQADGFCIPDEWECDGHPDCEDGSDEHNTCPAVTCRSNYFQC-TNKMCIPTSWLCDGENDCRDMSDEQNC----PTPPFSCPSGQWLCPTDQVCIDLDKVCNGQRDCPD--GADESPICNAD------DCALNNGGCSDD 1358 HSP 4 Score: 109.383 bits (272), Expect = 5.598e-21 Identity = 116/396 (29.29%), Postives = 163/396 (41.16%), Query Frame = 0
Query: 90 QFTCNNGRCIGQDLRCDGTPQCQDSTDEI-GCGAISKVAPGFFSSLNGRIIGLTF--------------DPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVE--CDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDE-QDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYEN-----ECRLKAMSCSSDQEI-QIYYEGP---------CETEEDPVEP---------QGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFD 443
QFTC NG CI +CDG CQD++DE+ A + F+ NGR + +++ C C+ T++ C+N RCI+ +CDG+ DC D + D CP EC Q +C CI CDG+++C D SDEN F + C QF+C G CI CD DC DE C++I T P C M VC + ++N E + +CS++Q +Y G C+ E+D + C ++++ C +G CI + CDR DC DG DE C +T +FTC +G+C CDG DC D SDE D
Sbjct: 2687 QFTCLNGNCISARWKCDGFNDCQDNSDELERVCAYHTCSSLDFTCDNGRCVPMSYVCDYTNDCGDNSDERDCAFPTCNPATEFTCSNG-RCINSDFVCDGHNDCRDNTTSDEINCPDREC-PAGQIKCERNNICIYPENLCDGFNNCGDNSDENPLFCADRTCAANQFQCD-QGKCIPESWVCDSIADCNDKTDEPPSCEDIVR---------------TCGPDQFT------CTNGNCVMQSVVCDGNNDCWDNSDEAPELQCGDRTCSTNQFTCPTWYPGHPRCLPLNFVCDGEKDCANAADELQNCPNRTCHMDEFACANGLCILIPYHCDRVNDCGDGSDELNCIYDTCSS----------SQFTCGNGACIYASYICDGENDCLDGSDEAD 3048 HSP 5 Score: 103.99 bits (258), Expect = 2.729e-19 Identity = 115/370 (31.08%), Postives = 145/370 (39.19%), Query Frame = 0
Query: 43 PEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCP---PSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFF-STNEIEEPVPTTVSPISIEVLPEDDC------PTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYY----EGPCETEED----PVEPQGCAVNQWQC-LDGSCIYLKQQCDRKYDCRDGEDEK 393
P C F C++ C + RCDG FDC D++DE C +C P FTC+N CI RCDG C D +DE C VP C + + C N +CI K +CDG DC D SDE C + F C D RC+ CDG DCLD SDE +C C ++F C CI + RCDG +DC DEQ C T P +E + P E DC CP+++ CR C++ I + E C D P P C QW C D CI L + C+ + DC DG DE
Sbjct: 1016 PPETCGDYSFECDEGRCRPNSYRCDGIFDCVDKTDEANCTDTGGTCSP-YAFTCDNKHCIYSSWRCDGFDDCGDGSDETDCPTR-----------------------VPTTC-SASYFTCDND-KCISKNWVCDGDNDCGDGSDEQNCGSTISSCPPGF-FLCPD-HRCLSSYYVCDGDQDCLDGSDEKNC-EFSCQSYEFACASGDQCISSYYRCDGVSDCRDHSDEQGCP--TPGPGLCHDDEFQCQADGFCIPDEWECDGHPDCEDGSDEHNTCPAVT--------------CRSNYFQCTNKMCIPTSWLCDGENDCRDMSDEQNCPTPPFSCPSGQWLCPTDQVCIDLDKVCNGQRDCPDGADES 1340 HSP 6 Score: 98.2117 bits (243), Expect = 2.093e-17 Identity = 89/246 (36.18%), Postives = 111/246 (45.12%), Query Frame = 0
Query: 42 IPEAQCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTC-NNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTF-----DPCVPG----ECDEE--TQWECANHRRCIDKRRLCDGYPDCPDQSDE---DAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDN 272
+ +A C+ +F C + CIN RCDG DC D SDE CPP + +F C +G CI CDG C D +DE P F+ G+ I + CV CD TQ CAN C + + C+G DC D SDE E CP QF+C + CI CD ++DC D+SDE C C QF C SG CI CD NDCG DE+ CD+
Sbjct: 20 LVQASCASNEFQCTNGQCINIEWRCDGTKDCTDDSDELNCPPPSCSSQEFRCVTSGECISLGFVCDGEEDCVDGSDEQRTCGGRTCNPDQFTCQEGQCIPSKYRCDHVTDCVDNSDERNCDYPQCTQKTCANG-ACYNNSQHCNGLQDCRDGSDETNCTTEHCPLH------QFECANG-FCIPIPFVCDHWNDCGDDSDEQGCEYQSCSGNQFTCS-SGRCIPQNWVCDKFNDCGDFSDERGCDS 256 HSP 7 Score: 83.5741 bits (205), Expect = 7.912e-13 Identity = 110/362 (30.39%), Postives = 141/362 (38.95%), Query Frame = 0
Query: 114 STDEIGCGAISKVAPGFFSSL-----NGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDEND--CFAVECDYWQFKCRISGLCIDTRRRCDGTNDC--GSDEDEQDCDNITENP--FFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPSEPEPTTADPFE 464
S+ + C + +APG + + + + F +C TQ+ C ++ +C+ CDG DC D SDE + CP C QFQC+D C C+GY DC D SDE+ C C QF+C+ CI CDG NDC GSDED + C T P F TN P+S ++DC G + C C D E C+T V QW +CD DC D DE+ C T CDPL +F C + C PI +CDG DC D SDE DC EP P T F
Sbjct: 3454 SSHHLTCSHLCLIAPGGEGATCECPDHFIALAVGFKIQCVADC-SSTQFRCGDNEKCVPIWWKCDGQSDCGDGSDE-PQTCPPRYC-PIGQFQCQDGN-CTFPGVICNGYQDCPDGSDEDAALCSDHRCAVNQFQCKNKN-CIPVSWHCDGVNDCSDGSDEDPESCAQKTCQPGEFKCTN-------GRCVPMSYVCDAQNDC------------GDGSDEPYDTCMGPDYKCDEDTEFS------CKTNYR-------CVPQWA-----------RCDGTNDCLDNSDEEGCEEVT---------CDPLGDFRCDNHRCVPIRWKCDGNNDCGDGSDEKDC---------EPRPCTESEFR 3749 HSP 8 Score: 81.6481 bits (200), Expect = 2.673e-12 Identity = 99/312 (31.73%), Postives = 126/312 (40.38%), Query Frame = 0
Query: 164 RCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDC---FAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVC--------GTDGLTYENECRLKAMSCSS-DQEIQIYYEGPCETE-EDPVEPQGCAV--------NQWQC-LDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDCPLGSTSKPS 453
RC CDG DC D++DE C + F C D + CI RCDG+ DC D SDE DC C F C + CI CDG NDCG DEQ+C + + P + P+ C + S VC G+D E C+ +C+S DQ I YY ++ D + QGC +++QC DG CI + +CD DC DG DE T + N F C + C P CDG DC D SDE +CP S PS
Sbjct: 1031 RCRPNSYRCDGIFDCVDKTDEANCTDTGGTC-SPYAFTC-DNKHCIYSSWRCDGFDDCGDGSDETDCPTRVPTTCSASYFTCD-NDKCISKNWVCDGDNDCGDGSDEQNCGSTISS----------------CPPGFFLCPDHRCLS-----SYYVCDGDQDCLDGSDEKNCEFSCQSYEFACASGDQCISSYYRCDGVSDCRDHSDEQGCPTPGPGLCHDDEFQCQADGFCIPDEWECDGHPDCEDGSDEH----NTCPAVTCRSN-----YFQCTNKMCIPTSWLCDGENDCRDMSDEQNCPTPPFSCPS 1309 HSP 9 Score: 78.9518 bits (193), Expect = 2.011e-11 Identity = 78/249 (31.33%), Postives = 108/249 (43.37%), Query Frame = 0
Query: 199 QFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQ-DCDNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFC--PSMSRPVCGTDGLTYENECRLKAMSCSSDQEIQIYYEGPCETEEDPVEPQGCAVNQWQCLDGSCIYLKQQCDRKYDCRDGEDEKYCSIETIDEIPAPPNCDPLDEFTCKDGSCRPIDTRCDGMVDCEDESDEFDC 444
+FQC + Q CI+ RCDG DC D+SDE +C C +F+C SG CI CDG DC DEQ C T NP T + + +P+ + ++ C P ++ C +G Y N + D E C TE C ++Q++C +G CI + CD DC D DE+ C ++ ++FTC G C P + CD DC D SDE C
Sbjct: 29 EFQCTNGQ-CINIEWRCDGTKDCTDDSDELNCPPPSCSSQEFRCVTSGECISLGFVCDGEEDCVDGSDEQRTCGGRTCNPDQFTCQEGQCIPSKYRCDHVTDCVDNSDERNCDYPQCTQKTCA-NGACYNNSQHCNGLQDCRDGSD----ETNCTTEH-------CPLHQFECANGFCIPIPFVCDHWNDCGDDSDEQGCEYQSCSG----------NQFTCSSGRCIPQNWVCDKFNDCGDFSDERGC 254 HSP 10 Score: 72.7886 bits (177), Expect = 1.838e-9 Identity = 94/330 (28.48%), Postives = 132/330 (40.00%), Query Frame = 0
Query: 84 SCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDEDAEMCPKVECGEEWQFQCRDKQRCIDKRRRCDGYSDCLDESDENDCFAVECDYWQFKCRISGLCIDTRRRCDGTNDCGSDEDEQDC--DNITENPFFSTNEIEEPVPTTVSPISIEVLPEDDCPTFCPSMSRPVCGTD----GLTYENECRLKAMSCSSDQEI-QIYYEGPCETEEDPVEPQGCAVNQWQCLDG--------SCIYLKQQCDRKYDCRDGEDEKYCSIE 398
SC N +F C NG+CI + RCDGT C D +DE+ C P C + ++ C CI +CDG DC D SDE C C + QF C++ Q CI + RCD +DC+D SDE +C +C Q C +G C + + C+G DC DE +C ++ + F N P+P + CG D G Y++ C +CSS + I Q + D + +GC + C G +CI Q CD + DC G DE + E
Sbjct: 24 SCASN-EFQCTNGQCINIEWRCDGTKDCTDDSDELNC--------------------------PPPSCSSQ-EFRCVTSGECISLGFVCDGEEDCVDGSDEQ-RTCGGRTCNPD-QFTCQEGQ-CIPSKYRCDHVTDCVDNSDERNCDYPQCT--QKTC-ANGACYNNSQHCNGLQDCRDGSDETNCTTEHCPLHQFECANGFCIPIPFVCDHWND-------------------CGDDSDEQGCEYQS-CSGNQFTCSSGRCIPQNWVCDKFNDCGDFSDERGCDSDSRDCYPGEWGCPGSTACIPFNQVCDGQPDCPGGTDETNTTAE 299 HSP 11 Score: 67.0106 bits (162), Expect = 9.408e-8 Identity = 49/139 (35.25%), Postives = 59/139 (42.45%), Query Frame = 0
Query: 46 QCSRTDFICEDLACINFALRCDGKFDCEDRSDEYQCPPSCDPNTQFTCNNGRCIGQDLRCDGTPQCQDSTDEIGCGAISKVAPGFFSSLNGRIIGLTFDPCVPGECDEETQWECANHRRCIDKRRLCDGYPDCPDQSDE 184
C F C + CI CD DC D SDE C QFTC++GRCI Q+ CD C D +DE GC + S+ C PGE W C CI ++CDG PDCP +DE
Sbjct: 180 HCPLHQFECANGFCIPIPFVCDHWNDCGDDSDEQGCEYQSCSGNQFTCSSGRCIPQNWVCDKFNDCGDFSDERGCDSDSR-------------------DCYPGE------WGCPGSTACIPFNQVCDGQPDCPGGTDE 293 The following BLAST results are available for this feature:
BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 11
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BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of basement membrane-specific heparan sulfate proteoglycan core vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold781_size98004:39709..50496- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold781_size98004-snap-gene-0.18 ID=maker-scaffold781_size98004-snap-gene-0.18|Name=basement membrane-specific heparan sulfate proteoglycan core|organism=Tigriopus kingsejongensis|type=gene|length=10788bp|location=Sequence derived from alignment at scaffold781_size98004:39709..50496- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'basement membrane-specific heparan sulfate proteoglycan core' has the following synonyms
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