BLAST of 250 kda substrate of akt vs. L. salmonis genes
Match: EMLSAG00000004974 (supercontig:LSalAtl2s:LSalAtl2s260:1491039:1496975:-1 gene:EMLSAG00000004974 transcript:EMLSAT00000004974 description:"maker-LSalAtl2s260-augustus-gene-14.34")

HSP 1 Score: 2290.38 bits (5934), Expect = 0.000e+0
Identity = 1211/2007 (60.34%), Postives = 1459/2007 (72.70%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKGAHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTALAAMTPDTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKMDPMLSCRVVVIKWVAQYTHITRSGAMGSSGTLAASNLGLNGTMDVLQSGL-NLSMSEKDTSFQVEHMSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKERDELDYAMTGKLSADMADPNDSGYRLRNDSYLGAIHKEHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRGQRGGPQDLKYS-------SSSIKQPVTPVSEVHPPILDDSSGAGSLGGAPQTQFRRQGSPYPRRKRISSETEYKSSPNERRQHQHVRFYPTNLR-----------RSMSEGYIKQPPTQLIGEYN-FHLESKRRHRSVESMYTYSRSESDGVGS-RSCSP---APSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQAC-------DPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKS-SKSRS------GSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELP-LMENVNSVQEIQTSSCQVRLILRDISGKFSWDSCAMNGLTKSPNGIIEMDYSKLAQMVLRSSTGSKSNVSH----QAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNAVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLFQYSGSSSRPASLIGSSSLGSQHLPNVLLDGNQPGTSPNQSHMFSSSHADIRPRTKTEGGGGGLSSPANHHSPHFLALNNNYDEETSPKQTIKQKLTFKASLPGGSAKRPLAIKEEDDL 1964
            MFSKK   SKK E+V+KS  KV+DPKRDTPTR KHL++FLD+++  EI  F E++ S +F+++YDAFI AE NLR KG HKAQREELEAVL+L E IL+YLPE LGRKW YH++ RLMSKLLHPGNSWK+LKREAMRLF+LWYQ L D AGEPLHA+FATLVPGFPSPY G+GLTALAA+TPDTQ+GP+ P EI PLIPPQSGER R SPDTD+TRIFL+VLLEL+VSQ+ LIEWKENK E++K+ L FL  KFK +YMPHIFP+FNW+ SLY PSL LPEVRKF  A +DTGDG KK+DPML+CRVVVIKWVAQYTH TR+     + TL  SN+GL  T      GL N S S       ++  S S FE       E N+VR +LY +R+NV+FVHEVYRQAFLLSF HSPAIKKVI+VYKDWIQMNV E PPFLLEP+H D  RD+   A+    S               DS  G+  +E   VRAGLQNVLQIF+TNAANVFLLE+S E PI L+EQVEMCKRVLNIYRYMVMNVKM+ARTWEQLLFILLQITQLT+PE+PPR+R+D LGGRLAQAIFQTLIVTWIKANLYVVVS ELWDQFLEVLSSLTLWEEL REW+KTMETLTRVLARQVY LDLNDLPLDRLSERAAKK+RG++GG  ++KYS       +S+ + P+   S +HP   ++ +       + +      GS    RKRI S + Y +S            +  N++           RSMSEG I +       +YN    E     ++  S ++  RS+S   G  R+  P     SS    +S+ DSPMQ D +T+S+GGLSD + HH  +S   +EPRSVMSGG+VKGWLPDVAVVLWRRMLGALGNIN IADTVIHAQIYKYLIEL+E++VKIRNNQGV+LDN  TPP P++VPP TIF PWC +AL+ P+ YQRG++YALRLLCL+T RPHD PL KTHL+QFYK+LH+GLT +K+D+MN LIK+TGPRFFSLMLPGYTAYILD+L+A N  ISTSELKGVPRTEATS+VG+LLAFP I+ DIP+LLPNS+ELSLISSAD+KDQI+G+LLKSG+KEPAGLARCISLSSLGIFVY+ELIH SFHPKIKEAIQVLLTALRFNNK+VAQIASDMLLLL DHV++ LD+YPEVPKKIVEVLA+TL  L P+ ++   EDEKRLL+SLLFCLGEW M+IP  +L+Q QEDG+SL Y VF ALQ A         P P G  E   RR  G +  +  + F   +  + S   M+ DFDPNIHVDNTKEGY S N+SP KS S+ +S       + E L +        DS  PVRLA+  +++HL+NH++HFPM  GAANLS L++E DD+PG   DEL M+ F  PNIQLF+LN++TL+SL+ELP +M++  +   IQT + QVR+ILRD+SGKFSWDS  + G    P+G+ +      +     SS  S   ++     Q +R+R  ++LP   N A+DMDNLDDLL Y+++TSPE  EE+G+P+N V  +  F+  VE D+IS V++QRT E+D+       L     P  P   E        FQ CR +F+ +G SSWE+RP++HLMKK ERL+RELKNLD  R RE HKIA+++V  GQEDK SIL N+SGSQAYEEF+SGLAW  ELE+HTGFM GL K+K+TGET PYYATSF+EVMFHVATRMPS +EES+LQKTRH+GNDEIHIVWSEH+RDYRRGILPTEFCDVLI+IYPL NKLFRIQ+SRK EVP+FGPLFNE IV  + LPGL+RATAINASRAKRSM+ ++QSHYEER+KALE+II NHK+ TT+EEFIT+V+SP  L NLFQ                                   PN     S    ++RPR+KTE      SS +          +  Y   TSPK    +K++FK     G  K  +  +E DD+
Sbjct:    1 MFSKKVGGSKKWEEVRKSGQKVLDPKRDTPTRTKHLKLFLDHSESREIAVFLESHNSEVFFVLYDAFILAETNLRTKGPHKAQREELEAVLFLLENILVYLPELLGRKWXYHAITRLMSKLLHPGNSWKILKREAMRLFLLWYQSLADAAGEPLHALFATLVPGFPSPYPGMGLTALAALTPDTQEGPITPGEIAPLIPPQSGERGR-SPDTDITRIFLDVLLELIVSQISLIEWKENKLEKRKKSLIFLLSKFKTFYMPHIFPDFNWSTSLYRPSLELPEVRKFTTALVDTGDGGKKIDPMLACRVVVIKWVAQYTHQTRNN---QANTLT-SNVGLYETGLEGSPGLSNFSGSGASFERTLDQQS-SSFE-------ESNLVREILYGDRDNVNFVHEVYRQAFLLSFRHSPAIKKVISVYKDWIQMNVPEXPPFLLEPIHPD--RDDYKKALLNGES---------------DSTDGS--REPTCVRAGLQNVLQIFVTNAANVFLLEVSAEDPIFLDEQVEMCKRVLNIYRYMVMNVKMEARTWEQLLFILLQITQLTLPETPPRKRDDFLGGRLAQAIFQTLIVTWIKANLYVVVSTELWDQFLEVLSSLTLWEELFREWSKTMETLTRVLARQVYNLDLNDLPLDRLSERAAKKKRGKQGGNHEMKYSHELKLSSTSTPQIPLASSSNLHPYYSENENYVSLSSSSNRIS---PGS----RKRIDSSSSYNNSLGSSNATTRTNDHSNNMKSKTSGRRKRMYRSMSEGNIVR------SDYNNIDDEIDGITKTFSSYFSKRRSKSVDFGIIRTSEPWGSRSSSPAPSSSINDSPMQFDPETMSDGGLSDLIGHHGSNSGGDNEPRSVMSGGSVKGWLPDVAVVLWRRMLGALGNINQIADTVIHAQIYKYLIELYEVIVKIRNNQGVTLDNMSTPPCPEYVPPVTIFTPWCFRALSXPDTYQRGRIYALRLLCLMTIRPHDNPLPKTHLIQFYKLLHQGLTQNKMDIMNILIKFTGPRFFSLMLPGYTAYILDYLYATNHIISTSELKGVPRTEATSIVGSLLAFPPIVRDIPVLLPNSNELSLISSADIKDQIVGILLKSGRKEPAGLARCISLSSLGIFVYTELIHKSFHPKIKEAIQVLLTALRFNNKSVAQIASDMLLLLTDHVQSLLDYYPEVPKKIVEVLARTLTGLAPKKDS---EDEKRLLMSLLFCLGEWTMKIPQQMLSQTQEDGQSLAYLVFNALQSAALSGCVSITPSPVGPTENNTRR-EGKNFEAFPQVFNDRSGTISSG--MLGDFDPNIHVDNTKEGYTSTNSSPQKSVSQPKSEHHLSRNNLEPLPLFSQNLSIMDSKCPVRLASNAIIAHLLNHVYHFPMGTGAANLSCLISEHDDVPGYVGDELSMEAFTSPNIQLFVLNDSTLVSLIELPVVMDDDPAFPGIQTGNSQVRIILRDLSGKFSWDSSILYG---PPDGLNKSMEDITSHPEFWSSCDSSFFLNKKRPCQPLRQRRASILPIYSNTADDMDNLDDLLSYLTYTSPEVMEEIGKPINTVSQSHFFDCVVEKDIISTVLNQRTSERDYTSNYGKNLLRPVSPPDPIGGEPK------FQFCRSIFNQMGWSSWEKRPHIHLMKKTERLIRELKNLDNIRGRETHKIAIIYVAAGQEDKHSILDNSSGSQAYEEFISGLAWXXELESHTGFMAGLXKNKSTGETXPYYATSFIEVMFHVATRMPSCTEESLLQKTRHLGNDEIHIVWSEHYRDYRRGILPTEFCDVLIIIYPLPNKLFRIQISRKLEVPFFGPLFNESIVDGKALPGLVRATAINASRAKRSMIPYFQSHYEERAKALEDIIKNHKESTTYEEFITKVYSPQTLQNLFQ-----------------------------------PNNPVRXSLXLQELRPRSKTEIQINRYSSVSE--------TDPLYQNGTSPKNM--KKMSFKNV--TGKVKTSVIKEENDDI 1900          

BLAST of 250 kda substrate of akt vs. L. salmonis genes
Match: EMLSAG00000002599 (supercontig:LSalAtl2s:LSalAtl2s151:403390:412004:-1 gene:EMLSAG00000002599 transcript:EMLSAT00000002599 description:"maker-LSalAtl2s151-augustus-gene-4.14")

HSP 1 Score: 94.7449 bits (234), Expect = 2.212e-19
Identity = 60/175 (34.29%), Postives = 90/175 (51.43%), Query Frame = 0

Query: 1600 LMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQ-EDKQSILSNASGSQAYEEFVSGLAWEVEL---ETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESML-QKTRHIGNDEIHIVWSE---HWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEV 1766
            L+  N+     +KNLD     E HKI V+++G  Q  D+  IL N SGS  Y +F+ GL   + +   +  T F+GGL   +  G  A  +    ++ +FHVAT MP+   +    +K RHIGN+   IV+++   H   Y+ G +  +F    IVI PL     R+ V  KPE+
Sbjct: 1302 LLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPEL 1476          

BLAST of 250 kda substrate of akt vs. L. salmonis genes
Match: EMLSAG00000009951 (supercontig:LSalAtl2s:LSalAtl2s64:61995:90540:1 gene:EMLSAG00000009951 transcript:EMLSAT00000009951 description:"maker-LSalAtl2s64-augustus-gene-1.7")

HSP 1 Score: 93.2041 bits (230), Expect = 4.820e-19
Identity = 69/241 (28.63%), Postives = 127/241 (52.70%), Query Frame = 0

Query: 1539 SQRTLEQDHVKKLAPELTYSAHPEVPPQ-LEENQDY-PSVFQQCRRLFSHLGLSSWEQRPNVHLM---KKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKS-KTTGETAPYYATSFLEVMFHVATRMP-SLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKN-KLFRIQVSRKPEVPYFGP 1771
            S   L ++H + L  + + + H  +P   LEE ++Y PS  +  + L   L L       NVH +   + +E +++  +++  K+    +K  V++  +GQ  ++ + +N   S A+EEF+  L   V L  H GF GGL  +   TG+ + Y      E+MFHV+T +P + ++   LQ+ RHIGND + +V+ +    +   ++ + F    +V+ P+K+   +++ VS + +VP FGP
Sbjct:  308 SSDGLPENHTRVLIRQNSGTLHRLIPDSILEEEEEYGPSPLKIVQILLPELSLE------NVHPVVCPRSSELIVKYDEHVLVKQ----NKFGVIYQKQGQVTEEELFANREHSPAFEEFLDILGNRVLLTEHKGFRGGLDTTYGQTGDVSVYETFHNREIMFHVSTLLPYTDNDPQQLQRKRHIGNDIVALVFQDDETPFCPDMITSHFLHAYVVVRPIKDGSDYKVSVSARKDVPNFGP 538          

BLAST of 250 kda substrate of akt vs. L. salmonis genes
Match: EMLSAG00000009942 (supercontig:LSalAtl2s:LSalAtl2s64:423747:425954:-1 gene:EMLSAG00000009942 transcript:EMLSAT00000009942 description:"augustus_masked-LSalAtl2s64-processed-gene-4.0")

HSP 1 Score: 88.5817 bits (218), Expect = 1.088e-17
Identity = 51/155 (32.90%), Postives = 86/155 (55.48%), Query Frame = 0

Query: 1623 HKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQ-KSKTTGETAPYYATSFLEVMFHVATRMPSLSEE-SMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLK---NKL-FRIQVSRKPEVPYFGP 1771
            +K  +V+   GQ  ++++  N S   A E F++ L  +V L +H G+ GGL  +   TG+ + +      E+MFHV+T +P    +   LQ+ RHIGND + I++ E    +   ++ + F    IV+ P++   NK+ ++I  S K +VPYFGP
Sbjct:  214 NKFGIVYQKVGQTTEEALFGNKSHCPAMETFLNLLGKKVMLXSHKGYRGGLDTQYGQTGKHSYFETFQGREIMFHVSTLLPXXDNDPQQLQRKRHIGNDIVAIIFQEGETPFSPDMITSHFLHAYIVVRPVQXSSNKVWYKIATSAKIDVPYFGP 368          

BLAST of 250 kda substrate of akt vs. L. salmonis genes
Match: EMLSAG00000009215 (supercontig:LSalAtl2s:LSalAtl2s586:192816:208940:-1 gene:EMLSAG00000009215 transcript:EMLSAT00000009215 description:"maker-LSalAtl2s586-augustus-gene-1.30")

HSP 1 Score: 79.337 bits (194), Expect = 1.027e-14
Identity = 61/221 (27.60%), Postives = 102/221 (46.15%), Query Frame = 0

Query: 1625 IAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGL-QKSKTTGETAPYYA-TSFLEVMFHVATRMP-SLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILP------TEFCDVLIVIYP----LKNKLFRIQVSRKPEVPYFGPLFNE--MIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFE 1830
            + +++  EGQ+ ++ + +N  G  A+ EF+  +  +V L+  + +  GL  K+ +TG  + Y    +  E+MFHV+T +P + S    L + R+IGND + +V+ E       G LP      ++F  V IV+       +N  + + VSR   VP FGP   E  M    +     +    INA  A      F       R + L+++  N    TT E
Sbjct:  465 VGLMYCREGQQTEEEMYNNEEGGAAFNEFLEMIGQKVRLKGFSKYKAGLCNKNDSTGLYSVYTEHENGSEIMFHVSTLLPFTNSNRQQLPRKRYIGNDIVTVVFQEP------GALPFIPNIKSQFQHVFIVVQAHNPCSENTQYSVAVSRSKSVPMFGPPIPEGAMFPKSKAFANFLLTKIINAENAAHHSDKFVSMAIRTRQELLKDLTTNFSSPTTLE 679          

BLAST of 250 kda substrate of akt vs. L. salmonis genes
Match: EMLSAG00000008967 (supercontig:LSalAtl2s:LSalAtl2s55:274420:296116:1 gene:EMLSAG00000008967 transcript:EMLSAT00000008967 description:"maker-LSalAtl2s55-snap-gene-3.15")

HSP 1 Score: 65.855 bits (159), Expect = 1.214e-10
Identity = 96/419 (22.91%), Postives = 177/419 (42.24%), Query Frame = 0

Query: 1327 VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAEC------DDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNSVQEIQTSSCQVRLILRDISGKFSWDSCAMNGLTKSPNGIIEMDYSKLAQMVLRSSTGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNAVPTTTLFENEV-ESDVISGVMSQRT-LEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPN---------VHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQA----YEEFVSGLAWEVELETHTGFMGGL------QKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGN 1718
            V+ +A+++L+ ++    +FP   GA ++SSL+ E       +++P      L  +   + + + F+++N+ +++++E    E+ N ++E   S   V L+LR  SG+F+W S  +  L +  +G+ +  Y    + +     G K+  S +                 E +D +           P CK +   P  AV +    E    E D++S +M Q+  LE D + K     +     +      +N++    FQ  R + SHLGL S     +         + L  +++  + +L+ LD    R      + +V  GQ+    ILSN   S++    + EF++ L W V +  HTG+ G +      Q   T     P +         HV     +L E S +  T  +GN
Sbjct:  985 VKDSAESLLNCILEQTEYFPSPCGAESISSLMNEVSCLQHLNNLPNSTASSLTXEEAIK-HYRYFVVDNSVIVAILE----ESFNDMEERIPS---VTLLLRCSSGRFAWSS-QLRHLPRHKSGLNKSSYDNPGRPLPLGEMGIKTEHSPKFF--------------PESIDRI-----------PLCKADKSIP--AVESVAGDERAAQELDLLSHLMDQQAALENDIINKQQGINSGQFQLDTECIAPDNKND---FQTARLILSHLGLLSLNALQDNQDSLVPRLISLNAESQEFMCDLEGLDHLSSRTWDTAHIFYVARGQKTVDEILSNVESSESVHPHFLEFLASLGWPVNVWRHTGWTGHISTAWRCQXEPTKPPKIPNHGGGIFNGDHHVLYWADALQEVSFVVPT--LGN 1362          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|147742932|sp|Q2PPJ7.2|RGPA2_HUMAN (RecName: Full=Ral GTPase-activating protein subunit alpha-2; AltName: Full=250 kDa substrate of Akt; Short=AS250; AltName: Full=p220)

HSP 1 Score: 1219.91 bits (3155), Expect = 0.000e+0
Identity = 753/1958 (38.46%), Postives = 1094/1958 (55.87%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKGAHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTALAAM---TPDTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKM----DPMLSCRVVVIKWVAQY---------THITRSGA----------MGSSGTLAASNLGLNGTMDVLQSGLNLS-MSEKDTSFQVEHMSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKERDELDYAMTGKLSADMADPNDSGYRL-----RNDSYLGAIHK------EHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRG-------QRGG------------------PQDLKYSSSS-----------IKQPVTPVSEVHPPILDDSSGAGSLGGAPQTQFRRQGS----PYPRRKRIS----SETEYKSSPNE-----------RRQHQHVRFYPTNLRRSMSEGYIKQPPTQLIGEYNFHLESKRRHRSVESMYTYSRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLS---DQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLL--LPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQP--------QEDGKS-LLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP-VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNSVQEIQTSSCQVRLILRDISGKFSWDSCAMNGLTK---SPNGIIEMDYSKLAQMVLRSSTGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNAVPTTTLFEN-EVESDVISGVMSQRTLEQDHVKK----LAPELTYSAHPE-VPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAP 1841
            MFS++ +      DVKKS  KV+DPK+D  TR KHLR  LDN D +++  FFE  YS I++I Y+ FI  E +L+ KG +K+QREEL+++L+LFEKIL +LPE +  +W Y S+G  + KLLH GNS K+ + E +RLF+LW Q L     E    +FA LVPGFP+     G   L  +   +P   D  + P EI PL+P  SGE+       D T  FL++LL+ +V Q   +EWK NK E Q     FLF  F+ YY+PH+FP F    ++Y P L++P +R  +P +I T    + +     P ++ RVV IKW+  +         T  T++G            G +    A  L   G  +  +S  N S +S++    ++ + SL   E+  R   E  MV+ +L + R  V+FV+EV+ QAFLL        +KV+ VY+ WI   + + P F+ EP   D  +++ +     +  +  A    SG++      R  S+  A+ +      E+  V+AG+Q +LQ+F+TN+AN+FLLE   E P+LL+EQV+ CK VL I+R M+M + M+ +TWEQ+L ILL+IT+  M +   ++ +D     LA  +F+TL+V WI+ANL V +S ELWD FL VLSSLT WEELI EWA  M++LT VLAR VY +++ +LPLD+LSE+  KK+RG       Q+G                   P   + +++S           ++Q  T V E        ++G+G L    Q Q  R  S    P P     S    +E    SS +E           +R+H+ +      +RRS S       P +L  + +      +     +S  T S +          S  P    E     ++P  +  D  +   L       S+ T  S    +  S+++GG++ GW PD A VLWRR+LG LG++N+I    IHA+++ YL EL   + KIR+N  +SLDN  +P PP  +PP  +FA W  KA  LP  Y+ GKL A RL+C +  R  D   +   LV FY V+H GLT    D++NT+I++  PRFFSL  PG++  + DF+ AA   +ST  L   PR+EA +V+G+L+ FP+  ++IPLL  +P  +E ++  + DVK  +I +LLK+  +EP   ARCI++ SLG+++  EL   + HP++KEAI V+   L+F NK VAQ+A D+L LL  + +    F   +P+K+ E+L  T+  L P  E    E +K+ ++SLL CL +WCM +P  +L  P        Q   ++ LL +++  L   C  G               S+T T++       A LS     +D+DP + + N K      ++ P++       S E  +++ V       ++  + L A+ V++HLVNHL H+P+S G A L SLV+E  D   +   EL  +VF+ PN+QLF+ N++TL+S ++ P    V        S   VR+I+RDISGK+SWD   + G  +   +PNG    + S L     R + G + + S                   E  D LD LL+ I HTSPEC       LN    T    N + E ++I  ++ Q   E ++++      A ++T    P  V P+          F  CR L   LG++SW++R N HL+KKN +LLRELKNLD+++CRE HKIAV ++ EGQEDK SILSN  GSQAYE+FV+GL WEV+L TH GFMGGLQ++ +TG+TAPYYATS +EV+FHV+TRMPS S++S+ +K RH+GNDE+HIVWSEH RDYRRGI+PT F DV I+IYP+KN +F I +++KPEVP+FGPLF+  IV  ++LP L+ AT INASRA + ++  YQS YEER+  LE II NH++  TFE+F  QVFSP+P
Sbjct:    1 MFSRRSH-----GDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDANDLKQFFETNYSQIYFIFYENFIALENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKI-RCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVMSSRGPCTLETLINPSPSVADVKIYPEEITPLLPAISGEKIAE----DQTCFFLQILLKYMVIQAASLEWK-NK-ENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLDIPHLRP-KPVYITTTRDNENIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAVQERAPELDGGGPTEQDKSHSNSSTLSDR----RLSNSSLCSIEEEHRMVYE--MVQRILLSTRGYVNFVNEVFHQAFLLPSCEIAVTRKVVQVYRKWI---LQDKPVFMEEPDRKDVAQEDAEKLGFSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEENTNVKAGVQALLQVFLTNSANIFLLEPCAEVPVLLKEQVDACKAVLIIFRRMIMELTMNKKTWEQMLQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLMVAWIRANLCVYISRELWDDFLGVLSSLTEWEELINEWANIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVLDPQKGTTVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNIVRQKATEVEECQQSENAPAAGSGHLTVGQQQQVLRSSSTSDIPEPLCSDSSQGQKAENTQNSSSSEPQPIQENKGHVKREHEGITIL---VRRSSS-------PAELDLKDDLQQTQGKCRERQKSESTNSDTTLGCTNEAELSMGPWQTCEEDPELNTPTDVVADADARHWLQLSPTDASNLTDSSECLTDDCSIIAGGSLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHARVFCYLYELWYKLAKIRDNLAISLDNQSSPSPPVLIPPLRMFASWLFKAATLPNEYKEGKLQAYRLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGFPGFSMLVGDFITAAARVLSTDILTA-PRSEAVTVLGSLVCFPNTYQEIPLLQSVPEVNE-AITGTEDVKHYLINILLKNATEEPNEYARCIAVCSLGVWICEELAQCTSHPQVKEAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKMAEILVATVAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSVLLHPVSTAVLEEQHSARAPLLDYIYRVLH-CCVCG---------------SSTYTQQSHYILTLADLSS----TDYDPFLPLANVK------SSEPVQ----YHSSAELGNLLTVEEEKKRRSLELIPLTARMVMAHLVNHLGHYPLSGGPAILHSLVSENHDNAHVEGSELSFEVFRSPNLQLFVFNDSTLISYLQTPTEGPVGG--SPVGSLSDVRVIVRDISGKYSWDGKVLYGPLEGCLAPNG---RNPSFLISSWHRDTFGPQKDSSQ----------------VEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSLTPCGMNYDQEKEIIEVILRQNAQEDEYIQSHNFDSAMKVTSQGQPSPVEPR--------GPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLVCATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSP 1865          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|284022067|sp|P86411.1|RGPA2_RAT (RecName: Full=Ral GTPase-activating protein subunit alpha-2; AltName: Full=250 kDa substrate of Akt; Short=AS250; AltName: Full=p220)

HSP 1 Score: 1203.73 bits (3113), Expect = 0.000e+0
Identity = 758/1941 (39.05%), Postives = 1087/1941 (56.00%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKGAHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTALAAM---TPDTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKM----DPMLSCRVVVIKWVAQY---------THITRSGA--------MGSSGTLAASNLGLNGTMDVLQSGLNLSMSEKDTSFQVEHMSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKERDELDYAMTGKLSADMADPNDSGYRL-----RNDSYLGAIHK------EHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRG-------QRGGPQDLKYSSSSIKQPVTPVSEVHPPILDDSSGAGSLGGAPQT---------QFRRQGS--------------PYPRRKRISSETEYKSSPNERRQH-QHVRFYPTNLRRSMSE--GYIK---QPPTQLI------GEYNFHLESKRRH-RSVESMYTYSRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPR-------SVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSA-DVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGH-----SNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNSVQEIQTSSCQVRLILRDISGKFSWDSCAMNGLTK---SPNGIIEMDYSKLAQMVLRSSTGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNA-VPTTTLFENEVESDVISGVMSQRTLEQDHVKKL----APELTYSAHPE-VPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAP 1841
            MFS++ +      DVKKS  KV+DPK+D  TR KHLR  LDN D  ++  FFE  YS I++I Y+ FIT E +L+ KG +K+QREEL+++L+LFEKIL +LPE +  +W Y S+G  + KLLH GNS K+ + E +RLF+LW Q L     E    +FA LVPGFP+     G   L  +   +P   D  + P EI PL+P  SGE+       D T  FL++LL+ +V Q   +EWK NK E Q     FLF  F+ YY+PH+FP F    ++Y P L +P +R  +P ++      + +     P ++ RVV IKW+  +         T  T++G             G +      L+G     Q   + S S   +  ++ + SL   E+  R   E  MV+ +L + R  V+FV+EV+RQAFLL        +KV+ VY+ WI  N    P F+ EP   D   ++ D     +  +       SG++      R  S+  A+ +      ++  V+AG Q +LQ+F+TNAANVFLLE   E P+LL EQV+ CK VL I+R M+M + M+ +TWEQ+L ILL+IT+  M +   +  +D+    LA  +F+TLIV WI+ANL V +S ELWD FL VLSSLT WEELI EW+  M++LT VLAR VY +++ +LPLD+LSE+  KK+RG       Q+G      +S S    P   V+E        +SGA  +  A  T         +F++  S              P PR    S  TE   S + + Q  ++ +   ++  +S+ E  G++    +  T L+       E +   ES++ H R  E   + S      VG  + +  P S  + T  +D  +    D +++      L      +S   E R       S+++GG + GW PD A VLWRR+LG LG++N+I    IHA+++ YL EL   + KIR+N  +SLDN  +P PP  +PP  +FA W  KA  LP  Y+ GKL A +L+C +  R  D   +   LV FY V+H GLT    DV+NT+IK   PRFFSL LPG++  + DF+ AA   +ST  L   PR+EA +++G+L+ FP+  ++IPLL        +++ A DVK  +I +LLK+  +EP   ARCI++ SLG+++  EL   + HP++K+AI V+   L+F NK VAQ+A D+L LL  + +    F   +P+K+ E+L  T+  L P  E    E +K+ ++SLL CL +WCM +P   L  P          V E L       P  AP +       H     S+T T++   T   A LS     +D+DP + + N +      N+ P++   S +     L++ + +       IP  L A+ V++HLVNHL H+P+S G A L SLV+E  D   +   EL  +VF+ PN+QLF+ N++TL+S ++ P             S   VR+I+RDISGK+SWD   + G  +   +P+G    + S         + G +SN+ H                  E  D LD LL+ I HTSPEC       LN   PT +    + E ++I  ++ Q T E ++V++     A ++T    P  V P+          F  CR L   LG++SW++R N HL+KKN +LLRELKNLD+++CRE HKIAV ++ EGQEDK SIL+N  GSQAYE+FV+GL WEV+L TH GFMGGLQ++ +TG+TAPYYATS +EV+FHV+TRMPS S++S+ +K RH+GNDE+HIVWSEH RDYRRGI+PT F DV I+IYP+KN +F I +++KPEVP+FGPLF+  IV  ++LP LI AT INASRA + ++  YQS YEER+  LE II NH++  TFE+F  QVFSP+P
Sbjct:    1 MFSRRSH-----GDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKI-RCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVLSSRGPCTLETLINPSPSVVDAKIYPEEITPLLPAVSGEKIAE----DQTCFFLQILLKYMVIQAASLEWK-NK-ENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRP-KPLYVTVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAIQEKVPELDGAGATEQDKSH-SNSSTLSDRRLSNSSLCSIEEEHRTVYE--MVQRILLSTRGYVNFVNEVFRQAFLLPSCEISITRKVVQVYRKWILQNK---PVFMEEPDKKDVAEEDADKLGLSETDSKEVSSESSGHKRSSSWGRTYSFTSAMSRGCVTEEDNTNVKAGAQAMLQVFLTNAANVFLLEPCVEVPMLLREQVDACKAVLIIFRRMIMELTMNQKTWEQMLQILLRITEAVMQKPKDKLVQDSFARSLAGLLFRTLIVAWIRANLCVYISRELWDDFLRVLSSLTEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCVLEPQKGTAVGRSFSLSWRSHP--DVTEPMRFRSATTSGAPGVEKARNTVRQKATEVEEFQQAESTAAADCDYLVVGQQPVPRSSSTSDITERLYSDSSQGQKVENSQNLSSSEPKSVQESKGHVTHEHEGVTILVRRSSSPAELDLKEESQQTHGRCRERQKSESTGSDMAVGYSNEAELPVSPWQ-TCEEDPELSTPTDAVADSDARHWLQLSPTDASNLTESRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHAKVFSYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFITAAARVLSTDML-AAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEPNECARCIAICSLGVWICEELAQCASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTA------VIEELH------PSRAPLLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSS----TDYDPFLPLANVR------NSEPVQYHSS-ADLGNLLTVEEEKKRRSVELIP--LTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFNDSTLISYLQTPAEGPAGGTS--GGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPSG---RNPSFQISGWHHHTCGPQSNLFH----------------GEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPSAMNCDQEKEIIEVILRQSTQEDEYVQRCHSNSAVKVTSQGQPSPVEPR--------GPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSP 1864          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|703556455|sp|O55007.2|RGPA1_RAT (RecName: Full=Ral GTPase-activating protein subunit alpha-1; AltName: Full=GAP-related-interacting partner to E12; Short=GRIPE; AltName: Full=GTPase-activating RapGAP domain-like 1; AltName: Full=Tuberin-like protein 1; AltName: Full=p240)

HSP 1 Score: 823.928 bits (2127), Expect = 0.000e+0
Identity = 463/1079 (42.91%), Postives = 667/1079 (61.82%), Query Frame = 0

Query:  794 GVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIH-------SSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIP--------HFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVS-DFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNS---VQEIQTSSCQVRLILRDISGKFSWDSCAM------NGLTKSPNGIIEMDYSKLAQMVLRSS-----TGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNA-VPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAP 1841
             +GSRS +P+PS+ L    M+   +QLD          ++L H  +        S    E  SVM+GG + GW  DVA V+WRRMLG LG++N I D  IHAQ++ YL EL + + KIR+N G+S DN  +P PP  +PP  I  PW  KA  L + Y++GKL+A +L+C    R  D   ++  L  FY ++H GL     D++NT+IK+  P+FFSL LPG T  I+DF+ AA   +++S     PR EA  ++G+L+ FP++  ++P L P++ ++++    DVK+ II  +L S + EP+G ARC++L SLGI++  EL+H S HP+IKEA+ V+  +L+F NK VA +A +ML +L  +      + P+ P KI+++L  T+  L P  EA   E +KRL++SLL CL +W M +P        H    + ++  KS+L  +++ L   C  G Q                     F       +S + + S D+DP +H+++ +E        PL S  S   S+    + +V++      I +  AA+TV++HLVNHL H+PMS G A L+S V E  D     + EL  ++F  PNIQ F+LNNTTL+S +++   E+V        + +++  VR+I+RD+SGK+SWDS  +      +GL +  + I+ M Y +  +    S+        K  +S Q +RR  ETV PT     E+ D LD+LLQY+  TSPEC +  G  LN   P       + E+DVI+ ++ Q T E++ V+K   +L   A  +  P  ++ Q   S F  CR L S LG++SW++R + HL+KKNE+LLREL+NLD+++CRE HKIAV +V EGQEDK SIL+N  GSQAYE+FV+GL WEV L  H GFMGGLQK+K+TG T PY+ATS +EV+FHV+TRMPS S++S+ +K RH+GNDE+HIVWSEH RDYRRGI+PTEF DVLIVIYP+KN +F IQ+ +KPEVP+FGPLF+  IV  +VLP ++R+TAINASRA +S++  YQ+ YEER++ L+ I+ +H + TTFE+F  QVFSPAP
Sbjct:  994 ALGSRSQTPSPST-LNIDHMEQKDLQLD----------EKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIIAAG-RVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPSTPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYAPRLQTYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQSVHSTGAENEKTEKSVLNCIYKVLH-GCVYGAQS--------------------FSHPKYFPISLSDLASVDYDPFMHLESLRE------PEPLHSPDSERSSK-LQPVTEVKTQMQQGLISI--AARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFDSPNIQFFVLNNTTLVSCIQIRSEESVPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSAILYGPPIVSGLPEPTSFILSMSYQEKPEEPPTSNECLEDITVKDGLSLQ-LRRFRETV-PTWSTIREEEDVLDELLQYLGTTSPECLQRTGISLNVPAPQPVCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQDEPTPQKPQ---SAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKYSILTNIGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSESDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRALKSLIPLYQNLYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAP 2025          

HSP 2 Score: 497.278 bits (1279), Expect = 2.217e-143
Identity = 292/711 (41.07%), Postives = 416/711 (58.51%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKGAHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTALAAMTP---DTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFI---------DTGDGTKKMDPMLSCRVVVIKWVAQY----------------THITRSGAMGSSGTLAASNLGLNGTMDVLQSGLNLSMSEKDTSFQVEH----MSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLH-------VDKERDELDYAMTGKLSADMADPNDSGYR--------LRNDSYLGAIHKEHLA----VRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPR------RREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSE 654
            MFSKKP+      DVKKS  KV+D K+D  TR KHLRI ++NAD  ++  FF+ ++SHI+Y+ ++ F+T E +L+QKG HK+QREEL+A+L++FEKIL  LPE + ++WQ+HS+G ++ KLLH GNS K ++RE +RLF+LW Q L D   +    MF+ L+PGF +P    G   L  +     + Q+  V   E+ PL+PPQSG++ +     DLT  FLE LL+ +V QV  +EWK NK E Q+R   FLF  FK +Y+P+IFP      SLY+P L++P+VR  +P ++         +T   TK  +P +  RV+VI+W+  +                  +       ++ +L +     N T+D          S  +TS   E      SL   ++     +E  +VR V  + R NV+FV E++RQAFLL    + A++KV+ VY++WIQ      P F+ EP         +    +  D+ ++ +      + N +            +N SY  A H    A    ++AG Q VLQ+FI N++N+FLLE + E   LL+E  +MCKR+LNIYRYMV+ V MD +TWEQ+L +LL++T+  +  S         ++  TL GRLA  +FQTLIV WIKANL V +S ELWD  L VLSSLT WEEL  EW+ TMETLT+VLAR +Y LDL+DLPLD+LSE
Sbjct:    1 MFSKKPH-----GDVKKSTQKVLDTKKDALTRLKHLRIVIENADSIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKG-HKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLK-IRREGVRLFLLWLQALQDNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEVTPLVPPQSGDKGQ----EDLTSYFLEALLKYIVIQVKSLEWK-NK-ENQERGFSFLFSHFKKFYLPYIFPNLCKENSLYHPVLDIPQVRP-KPHYVMVKKDAETNETIYCTK--EPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREESKNDTVDKADKTAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIE--IVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQE--GKPLFMQEPEETVITSSDIPCSENVTDHDISIEDGEKREEENGTNISEHVRNSTWTKNGSYQEAFHVSEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSE 691          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|51315850|sp|Q6GYQ0.1|RGPA1_HUMAN (RecName: Full=Ral GTPase-activating protein subunit alpha-1; AltName: Full=GAP-related-interacting partner to E12; Short=GRIPE; AltName: Full=GTPase-activating Rap/Ran-GAP domain-like 1; AltName: Full=Tuberin-like protein 1; AltName: Full=p240)

HSP 1 Score: 822.772 bits (2124), Expect = 0.000e+0
Identity = 466/1082 (43.07%), Postives = 671/1082 (62.01%), Query Frame = 0

Query:  795 VGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIH-------SSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQP--------QEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVS-DFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNS---VQEIQTSSCQVRLILRDISGKFSWDSCAM------NGLTKSPNGIIEMDYSKLAQMVLRSS-----TGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNA-VPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNL 1845
            +GSRS +P+PS+ L    M+   +QLD          ++L H  +        S    E  SVM+GG + GW  DVA V+WRRMLG LG++N I D  IHAQ++ YL EL + + KIR+N G+S DN  +P PP  +PP  I  PW  KA  L + Y++GKL+A +L+C    R  D   ++  L  FY ++H GL     D++NT+IK+  P+FFSL LPG T  I+DF+ AA   +++S     PR EA  ++G+L+ FP++  ++P L PN  ++++    DVK+ II  +L S + EP+G ARC++L SLGI++  EL+H S HP+IKEA+ V+  +L+F NK VA +A +ML +L  +V     + P+ P KI+++L  T+  L P  EA   E +KRL++SLL CL +W M +P   L QP         +  KS+L  +++ L   C  G Q                     F       +S + + S D+DP +H+++ KE        PL S  S   S+    + +V++      I +  AA+TV++HLVNHL H+PMS G A L+S V E  D     + EL  ++F+ PNIQ F+LNNTTL+S +++   EN+        + +++  VR+I+RD+SGK+SWDS  +      +GL++  + ++ + + +  +    S+        K  +S Q  R R ETV PT +   ++ D LD+LLQY+  TSPEC +  G  LN   P       + E+DVI+ ++ Q T E++ V+K   +L   A  +  P  ++ Q   S F  CR L S LG++SW++R + HL+KKNE+LLREL+NLD+++CRE HKIAV +V EGQEDK SIL+N  GSQAYE+FV+GL WEV L  H GFMGGLQK+K+TG T PY+ATS +EV+FHV+TRMPS S++S+ +K RH+GNDE+HIVWSEH RDYRRGI+PTEF DVLIVIYP+KN +F IQ+ +KPEVP+FGPLF+  IV  +VLP ++RATAINASRA +S++  YQ+ YEER++ L+ I+ +H + TTFE+F  QVFSPAP  +L
Sbjct:  996 LGSRSQTPSPST-LNIDHMEQKDLQLD----------EKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAG-RVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPSLHPNIPDVAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPFHATGAESDKTEKSVLNCIYKVLH-GCVYGAQC--------------------FSNPRYFPMSLSDLASVDYDPFMHLESLKE------PEPLHSPDSERSSK-LQPVTEVKTQMQHGLISI--AARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRSEENMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPPVSGLSEPTSFMLSLSHQEKPEEPPTSNECLEDITVKDGLSLQFKRFR-ETV-PTWDTIRDEEDVLDELLQYLGVTSPECLQRTGISLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAVEQDEPIPQKPQ---SAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHL 2030          

HSP 2 Score: 502.286 bits (1292), Expect = 4.320e-145
Identity = 298/712 (41.85%), Postives = 420/712 (58.99%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKGAHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTALAAMTP---DTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFI------DTGDGTK-KMDPMLSCRVVVIKWVAQY---------THITRSGAMG---------SSGTLAASNLGLNGTMDVLQSGLNLSMSEKDTSFQVEH----MSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKERDEL---------DYAMTGKLSADMADPNDSGYRLRNDS------YLGAIHKE-----HLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQ--LTMPESP----PRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSE 654
            MFSKKP+      DVKKS  KV+D K+D  TR KHLRI ++NA+  ++  FF+ ++SHI+Y+ ++ F+T E +L+QKG HK+QREEL+A+L++FEKIL  LPE + ++WQ+HS+G ++ KLLH GNS K+ +RE +RLF+LW Q L +   +    MF+ L+PGF +P    G   L  +     + Q+  V   EI PL+PPQSG++ +     DLT  FLE LL+ +V QV  +EWK NK E Q+R   FLF  FK YY+P+IFP      SLY+P L++P++R  +P ++      +T +      +P +  RV+VI+W+  +          HI   G  G         ++ +L +     N   D          S  +TS   E      SL   ++     +E  +VR V  + R NV+FV E++RQAFLL    + A++KV+ VY++WIQ    E P F+ EP  +     +L         D +M      +  +  ++   +RN S      Y GA+H          +RAG Q VLQ+FI N++N+FLLE + E   LL+E  +MCKR+LNIYRYMV+ V MD +TWEQ+L +LL++T+  L MP         ++  TL GRLA  +FQTLIV WIKANL V +S ELWD  L VLSSLT WEEL  EW+ TMETLT+VLAR +Y LDL+DLPLD+LSE
Sbjct:    1 MFSKKPH-----GDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKG-HKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKI-RREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSAPQSEHGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQE----DLTSYFLEALLKYIVIQVKSLEWK-NK-ENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPILDIPQMRP-KPHYVVIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHI--PGMEGEVLPKNIQRAAASLVSREESKNDNADKTDRTTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIE--IVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQE--EKPLFMQEPEEIVITSSDLPCIENVTDHDISMEEGEKREEENGTNTADHVRNSSWAKNGSYQGALHNASEEATEQNIRAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSE 692          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|51315849|sp|Q6GYP7.1|RGPA1_MOUSE (RecName: Full=Ral GTPase-activating protein subunit alpha-1; AltName: Full=GAP-related-interacting partner to E12; Short=GRIPE; AltName: Full=GTPase-activating RapGAP domain-like 1; AltName: Full=Tuberin-like protein 1; AltName: Full=p240)

HSP 1 Score: 820.846 bits (2119), Expect = 0.000e+0
Identity = 472/1105 (42.71%), Postives = 673/1105 (60.90%), Query Frame = 0

Query:  770 FHLESKRRHRSVESMYTYSRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLS-DQLSHHTIH--------SSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQP--------QEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVS-DFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNS---VQEIQTSSCQVRLILRDISGKFSWDSCAM------NGLTKSPNGIIEMDYSKLAQMVLRSS-----TGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNA-VPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAP 1841
             HL+S+    S  +   +S   +   GS S SP   S L   S   SP  L    I +  L  D+  HH++         S    E  SVM+GG + GW  DVA V+WRRMLG LG++N I D  IHAQ++ YL EL + + KIR+N G+S DN  +P PP  +PP  I  PW  KA  L + Y++GKL+A +L+C    R  D   ++  L  FY ++H GL     D++NT+IK+  P+FFSL LPG T  I+DF+ AA   +++S     PR EA  ++G+L+ FP++  ++P L PN  ++++    DVK+ II  +L S + EP+G ARC++L SLGI++  EL+H S HP+IKEA+ V+  +L+F NK VA +A +ML +L  +V       P+ P KI+++L  T+  L P  EA   E +KRL++SLL CL +W M +P   L QP         +  KS+L  +++ L   C  G Q                     F       +S + + S D+DP +H+++ KE        PL S  S   S+    + +V++      I +  AA+TV++HLVNHL H+PMS G A L+S V E  D     + EL  ++F+ PNIQ F+LNNTTL+S +++   E++        + +++  VR+I+RD+SGK+SWDS  +      +GL +  + I+ M   +  +    S+        K  +S Q +RR  ETV PT     E+ D LD+LLQY+  TSPEC +  G  LN   P       + E+DVI+ ++ Q T E++ V+K   +L   A  +  P  ++ Q   S F  CR L S LG++SW++R + HL+KKNE+LLREL+NLD+++CRE HKIAV +V EGQEDK SIL+N  GSQAYE+FV+GL WEV L  H GFMGGLQK+++TG T PY+ATS +EV+FHV+TRMPS S++S+ +K RH+GNDE+HIVWSEH RDYRRGI+PTEF DVLIVIYP+KN +F IQ+ +KPEVP+FGPLF+  IV  +VLP ++R+TAINASRA +S++  YQ+ YEER++ L+ I+ +H + TTFE+F  QVFSPAP
Sbjct:  961 LHLDSET---SSLNQQAFSAEVATVTGSESASPV-HSALGSRSQTPSPSTLSRAHIEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIIAAG-RVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIHQPQSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKTEKSVLNCIYKVLH-GCVYGAQS--------------------FSNPKYFPISLSDLASVDYDPFMHLESLKE------PEPLHSPDSERSSK-LQPVTEVKTQMQQGLISI--AARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRSEESMPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSAILYGPPIVSGLPEPTSFILSMSDQEKPEEPPTSNECLEDIAVKDGLSLQ-LRRFRETV-PTWSTIREEEDVLDELLQYLGTTSPECLQRTGISLNVPAPQPLCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQDEPTPQKPQ---SAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKYSILTNIGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNRSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAP 2025          

HSP 2 Score: 498.049 bits (1281), Expect = 9.927e-144
Identity = 294/719 (40.89%), Postives = 418/719 (58.14%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKGAHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTALAAMTPDTQDGPVNP-----------AEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFI---------DTGDGTKKMDPMLSCRVVVIKWVAQY----------------THITRSGAMGSSGTLAASNLGLNGTMDVLQSGLNLSMSEKDTSFQVEH----MSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEP---------LHVDKERDELDYAMTGKLSADMADPNDSGYRLRND------SYLGAIHKEHLA----VRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPR------RREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSE 654
            MFSKKP+      DVKKS  KV+D K+D  TR KHLRI ++NA+  ++  FF+ ++SHI+Y+ ++ F+T E +L+QKG HK+QREEL+A+L++FEKIL  LPE + ++WQ+HS+G ++ KLLH GNS K ++RE +RLF+LW Q L D   +    MF+ L+PGF +P    G        P T D  +NP            E+ PL+PPQSG++ +     DLT  FLE LL+ +V QV  +EWK NK E Q+R   FLF  FK +Y+P+IFP      SLY+P L++P++R  +P ++         +T   TK  +P +  RV+VI+W+  +                  +       ++ +L +     N T+D +        S  +TS   E      SL   ++     +E  +VR V  + R NV+FV E++RQAFLL    + A++KV+ VY++WIQ    E P F+ EP         +   +   + D A+      +  +   +   +RN       SY  A H    A    ++AG Q VLQ+FI N++N+FLLE + E   LL+E  +MCKR+LNIYRYMV+ V MD +TWEQ+L +LL++T+  +  S         ++  TL GRLA  +FQTLIV WIKANL V +S ELWD  L VLSSLT WEEL  EW+ TMETLT+VLAR +Y LDL+DLPLD+LSE
Sbjct:    1 MFSKKPH-----GDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKG-HKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLK-IRREGVRLFLLWLQALQDNCSKEQLWMFSCLIPGFSAPQSEYG--------PRTLDNLINPPLSLQETQVTIEEVTPLVPPQSGDKGQ----EDLTSYFLEALLKYIVIQVKSLEWK-NK-ENQERGFSFLFSHFKKFYLPYIFPNICKENSLYHPVLDIPQIRP-KPHYVMIKKDAETNETIYCTK--EPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREESKNDTVDKVDKSAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIE--IVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQE--EKPLFMQEPEDTAITCSDIPCSETVADHDSAIEDGEKREEENGTSTSEHVRNSSWTKNGSYQEAFHVCEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKSMTLAGRLAGPLFQTLIVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSE 691          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|147742933|sp|A3KGS3.2|RGPA2_MOUSE (RecName: Full=Ral GTPase-activating protein subunit alpha-2; AltName: Full=250 kDa substrate of Akt; Short=AS250; AltName: Full=P220)

HSP 1 Score: 755.747 bits (1950), Expect = 0.000e+0
Identity = 429/1005 (42.69%), Postives = 605/1005 (60.20%), Query Frame = 0

Query:  849 SVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSA-DVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGH-----SNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNSVQEIQTSSCQVRLILRDISGKFSWDSCAMNGLTK---SPNGIIEMDYSKLAQMVLRSSTGSKSNVSHQAMRRRNETVLPTQE--NAAEDMDNLDDLLQYISHTSPECKEEVGRPLNA-VPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAP 1841
            S+++GG + GW PD A VLWRR+LG LG++N+I    IHA+++ YL EL   + KIR+N  +SLDN  +P PP  +PP  +FA W  KA  LP  Y+ GKL A +L+C +  R  D   +   LV FY V+H GLT    DV+NT+IK   PRFFSL LPG++  + DF+ AA   +ST  L   PR+EA +++G+L+ FP+  ++IPLL        +++ A DVK  +I +LLK+  +EP   ARCI++ SLG+++  EL   + HP++K+AI V+   L+F NK VAQ+A D+L LL  + +    F   +P+K+ E+L  T+  L P  E    E +K+ ++SLL CL +WCM +P   L  P          V E L       P  AP +       H     S+T T++   T   A LS     +D+DP + + N +      N+ P++   S +     L++ + +       IP  L A+ V++HLVNHL H+P+S G A L SLV+E  D   +   EL  +VF+ PN+QLF+ N++TL+S ++ P             S   VR+I+RDISGK+SWD   + G  +   +PNG                      N S Q     + T  P ++  N  E  D LD LL+ I HTSPEC       LN   PT      + E  ++  ++ Q   E ++V++   + + +   +  P   E +     F  CR L   LG++SW++R N HL+KKN +LLRELKNLD+++CRE HKIAV ++ EGQEDK SIL+N  GSQAYE+FV+GL WEV+L TH GFMGGLQ++ +TG+TAPYYATS +EV+FHV+TRMPS S++S+ +K RH+GNDE+HIVWSEH RDYRRGI+PT F DV I+IYP+KN +F I +++KPEVP+FGPLF+  IV  ++LP LI AT INASRA + ++  YQS YEER+  LE II NH++  TFE+F  QVFSP+P
Sbjct:  912 SIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFITAAARVLSTDML-AAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEPNECARCIAICSLGVWICEELAQSASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTA------VLEELH------PSRAPLLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSS----TDYDPFLPLANVR------NSEPIQYHSS-ADLGNLLTVEEEKKRRSVELIP--LTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFNDSTLISYLQTPAEGPAGGTS--GGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPNG---------------------RNPSFQISGWHHHTCGPQKDLFNGEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPCAMNWDQEKAIMEVILRQSAQEDEYVQRCNSDSSVTVTSQGQPSPVEPR---GPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSP 1864          

HSP 2 Score: 473.396 bits (1217), Expect = 5.667e-136
Identity = 281/698 (40.26%), Postives = 400/698 (57.31%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKGAHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTALAAM---TPDTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKM----DPMLSCRVVVIKWVAQY---------THITRSGA--------MGSSGTLAASNLGLNGTMDVLQSGLNLSMSEKDTSFQVEHMSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKERDELDYAMTGKLSADMADPNDSGYRL-----RNDSYLGAIHK------EHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRGQ 663
            MFS++ +      DVKKS  KV+DPK+D  TR KHLR  LDN D  ++  FFE  YS I++I Y+ FIT E +L+ KG +K+QREEL+++L+LFEKIL +LPE +  +W Y S+G  + KLLH GNS K+ + E +RLF+LW Q L     E    +FA LVPGFP+     G   L  +   +P   D  + P EI PL+P  SGE+       D T  FL++LL+ +V Q   +EWK NK E Q     FLF  F+ YY+PH+FP F    ++Y P L +P +R  +P ++      + +     P ++ RVV IKW+  +         T  T++G             G +      L+G     Q   + S S   +  ++ + SL   E+  R   E  MV+ +L + R  V+FV+EV+RQAFLL        +KV+ VY+ WI  N    P F+ EP   D  +++ D     +  +  A    SG++      R  S+  A+ +      ++  V+AG Q +LQ+F+TNAANVFLLE   E P+LL EQV+  K VL I+R M+M + M+ +TWEQ+L ILL+IT+  M +   +  +D     LA  +F+TLIV WI+ANL V +S ELWD FL VLSSLT WEELI EW+  M++LT VLAR VY +++ +LPLD+LSE+  KK+RG+
Sbjct:    1 MFSRRSH-----GDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDASDLKQFFETNYSQIYFIFYENFITLENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFFRWHYQSIGSTLKKLLHTGNSIKI-RCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFPAVLSSRGPCTLETLINPSPSIVDAKIYPEEITPLLPAISGEKIAE----DQTCFFLQILLKYMVIQAASLEWK-NK-ENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLEIPHLRP-KPVYVTVTRDNETIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKIIQTVGGGAIQEKVPELDGAGSTEQDKSH-SNSSTLSDRRLSNSSLCSIEEEHRTVYE--MVQRILLSTRGYVNFVNEVFRQAFLLPSCEISVTRKVVQVYRKWILQNK---PVFMEEPDKKDVAQEDADKLGLSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEDNTNVKAGAQAMLQVFLTNAANVFLLEPCAEVPMLLREQVDASKAVLIIFRRMIMELTMNQKTWEQMLQILLRITEAVMQKPKDKHVKDLFAQSLAGLLFRTLIVAWIRANLCVYISRELWDDFLRVLSSLTEWEELITEWSNIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGK 679          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|74868143|sp|Q9VB98.2|Y5521_DROME (RecName: Full=Probable Rho GTPase-activating protein CG5521)

HSP 1 Score: 728.783 bits (1880), Expect = 0.000e+0
Identity = 452/1170 (38.63%), Postives = 660/1170 (56.41%), Query Frame = 0

Query:  742 RFYPTNLRRSMSEGYIKQPPTQLIGEYNFHLESKRRHRSVESMYTYSRSESD-GVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKY-TGPRFFSLMLPGYTAYILDFLFAANCTISTSEL-KGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELI--------------------HGSFHPK--------IKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQP-QEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNI-HVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGL---GTDELCMDVFQQP------NIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM----NGLTKSPNGIIEMDYSK-LAQMVLRSSTGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNAVPTTTLFENEVESDVISGVMSQRTLEQDHV----KKLAPELTYSAHPEVPPQLEENQDYPSV-------------FQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNL 1845
            R  P +LRR+MS                  L+S  R    E   +Y   +++ G GSRS SP  SSG+E  S+KD+ +Q+D    S    +        +S +    R ++ GG+ +GW PD   ++W+RMLGALG++N I    +HAQ++ +L+E+ + ++KI+ NQG+S DN  T P P  VPP  I APWC  +L+L  ++++GKL+AL+LLC L  +     ++   L  FY  LH+ LTG   D++  ++K+  GPR  SL+LPG+T  +LD + A+   +++ E+ +  PR E  +++G+LL +PS L    +L P   +  L+  +D++D I+ ++L+  ++EP+  ARCI+LS LG ++   L                     H   HPK        IKE +QVLL AL+F +  +A +A D L L A+  +  L     VP+ I+  + K   +L  +      + +K +L SL+ CLGE+CM IP  I+  P  E G +L+  V   L Q       GAP         H         +TA            DFD +I H D   +G +     P  + ++    Q+ ++ I             +L AK V  HLV H+ HFPM  GA+ LSS+V E DDI      G  E   D  + P      N+QLF+LN+  + S +ELP ++         + T+  QVR+++RD++GK  WD+  +        + P    ++ +S+ L  M     T + S      +R R   VLP  ++ A D+D LDD+L YI HTSPEC       LNA PT +      E+  IS +++QR LEQ+ V    +  +P L +++      Q ++   + +              FQ CR LFSHLGL+ WE+R   HL++++E+L+REL+N+D ++CRE HK+AV++V  GQEDK SIL N SGS  YE FVS L WE++LETH GF+GGL + +  G TAPYYAT FLEV++HVATRMPS S E+ML KTRH+GNDE+HIVWSEH RDYRR ILPTEFCDVLIV+YPL+N LFR+ V+RKPEVP+FGPL NE +V    L  LIRATAINASR KR+ L  YQ  YEER+++L+++ + +K+ TTFE+F +++++P PL+ L
Sbjct:  753 RINPQDLRRAMS------------------LDSLARKGDAEETDSYQEGDNESGAGSRSPSPTASSGIEGGSIKDAQLQID---ASLDDANSGSYGSGSNSGSISGRRCIILGGSAEGWHPDSTSIMWKRMLGALGDVNRIPKADLHAQVFMHLLEMTQNLIKIKQNQGISTDNQNTQPMPPLVPPIGIVAPWCYGSLSLDRSFKKGKLWALQLLCSLAIQ---GAVNMQQLPLFYHALHQLLTGEDRDLIYAILKHLEGPRLLSLLLPGHTLLLLDLVHASAILLTSLEVSRSTPRAEVAALLGSLLCYPSSLLTRSVLQPTPQKFELMECSDLQDHILNIVLRCARREPSAKARCIALSQLGQWLLMRLSQPLPASNSGRANLFQQAVPHHKDVHPKASSVYNPRIKEVLQVLLQALQFKHHTIAIVAVDSLKLCAERGRQ-LAAIERVPQLIITAICK---ALEIQSVTKPKDSDKVVLTSLMLCLGEFCMAIPAPIMLTPFNEQGDTLVLQVLRVLLQVAS----GAPR--------HERVK-----LTAD----------DDFDMHIAHDDLQGDGRL-----PEATYQTSETIQKCITAI-------------KLCAKAVSMHLVTHIGHFPMGIGASRLSSMVEEQDDIGNAAYGGQVETRRDSVELPSVVSAQNMQLFMLNSGLVASFIELPTLKLPGGGITAGLVTADKQVRVLMRDLNGKACWDASILYSEPRNAEEPPKTTPKIQHSQPLDSMATSMVTHTPS--PRHTLRHRPAGVLPLAKDMAPDLDQLDDMLAYIGHTSPECVAPTVSQLNA-PTASPLSGNQEAQAISVILNQRLLEQEFVTHSTQAPSPALRHASSNSSLQQPDQRSLHSTTASFDSLPTRTEMPFQYCRLLFSHLGLAGWERRSRTHLLQRSEKLMRELRNVDLQKCRETHKMAVIYVAAGQEDKGSILRNTSGSSTYEMFVSALGWEIDLETHNGFLGGLPR-QGCGATAPYYATPFLEVVYHVATRMPSDSSEAMLLKTRHLGNDEVHIVWSEHNRDYRRDILPTEFCDVLIVVYPLRNGLFRVTVNRKPEVPWFGPLANESVVSGACLATLIRATAINASRTKRAALPLYQQFYEERNRSLDSVSSRYKESTTFEDFASRIYNPMPLSTL 1845          

HSP 2 Score: 497.664 bits (1280), Expect = 7.531e-144
Identity = 300/704 (42.61%), Postives = 401/704 (56.96%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKGAHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGL----------------GLTALAAMTPDTQDG----PVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKMDPMLSCRVVVIKWVAQYTHITRSGAMGSSGTLAASNLGLNGTMDVLQSGLNLSMSEKDTSFQVEHMSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSP---AIKKVITVYKDWIQMNVVELPPFLLEPLHVDKERDELDYAMTGKLSADMADPNDSGYRLRNDSYLGAI--HKEHLAVRAGLQNVLQIFITNAANVFLLE---ISPEYPIL--------LEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQIT-------QLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRR 661
            MF+KK +      DVKKS  K+ D K+D+ +R +HLR+ LDN +L E    FE  YSH+++I+YD FI AE NL+QK  HKA REEL+  L+L EKIL  LPE L R+WQ HS+ R+M+KLLH GNS KL +RE +R F+LWYQ LG+ A   +HAM+A L+PG   P +G+                 + A   M     D     PV  +E+  L+PP S E+    PD    R  LEVLL  +V     + W++N++++  R   FL  +F   ++P   P F+ + S+YNP L+LP +R             KK + M SC VV+I WV+++TH                         +L   L+ ++  +D    V+ + L  ++       +G +VR VLY NREN++FVHEVYRQAFLL+F+  P   AI+  I VY+DW  M     PPFLLE                       A       RLR  SY+GAI   K+ L VRAG QNVLQ+F+TNAANVFL+    ++  +P          LEEQ E+CK+VLN+YR MVMN +MD RTWEQLL +LLQ+T       Q T+P      +  TLGG L  AIFQTLIVTWI+A+  V V+  LW++FL VL SLT  EELI EW KT++TLTRV +R  Y ++L DLPLDR++E  A+KRR
Sbjct:    1 MFTKKSH-----ADVKKSTAKLQDSKKDSASRLRHLRMILDNVELEESKCLFETNYSHVYFILYDTFIQAEANLKQK-VHKAHREELDGSLWLLEKILCLLPELLARRWQCHSLSRIMAKLLHLGNSPKL-RREGVRYFLLWYQTLGENAPGYVHAMYADLIPGLIVPQKGVVGPDTEFSASDFLTHPNMKADGGMASVFHDNAFSHPVQSSEVVALLPPSSSEK-SAPPDP---RDGLEVLLNSMVHTAACLRWRDNRAQKDHRAFAFLLQRFMDVFLPVFSPNFDVSCSIYNPRLDLPVMRSI----------NKKEEVMASCVVVLINWVSRFTH-----------------------ERLLSHRLDCTLHIED----VDQVRLHGYQ-------QGLIVRDVLYVNRENINFVHEVYRQAFLLNFTSKPQIEAIRTAIAVYRDW--MTGETPPPFLLE-------------PNDDPPPPSNAGGTPRSQRLRTPSYVGAIAGSKDQLVVRAGRQNVLQVFVTNAANVFLVNTANLNICFPTRSRSYRSTPLEEQTEICKKVLNVYRTMVMNTEMDTRTWEQLLMVLLQVTSIVLHQNQHTLPSG--TNKSATLGGILGSAIFQTLIVTWIRAHTKVPVNVLLWEKFLNVLQSLTHREELIIEWNKTIQTLTRVFSRYTYGINLLDLPLDRVAESRAEKRR 632          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|284022066|sp|P86409.1|RGPA1_PIG (RecName: Full=Ral GTPase-activating protein subunit alpha-1; AltName: Full=GAP-related-interacting partner to E12; Short=GRIPE; AltName: Full=GTPase-activating Rap/Ran-GAP domain-like 1; AltName: Full=Tuberin-like protein 1; AltName: Full=p240)

HSP 1 Score: 257.684 bits (657), Expect = 2.827e-75
Identity = 142/326 (43.56%), Postives = 209/326 (64.11%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKGAHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTALAAMTP---DTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFI------DTGDGTK-KMDPMLSCRVVVIKWVAQY 316
            MFSKKP+      DVKKS  KV+D K+D  TR KHLRI ++NA+  ++  FF+ ++SHI+Y+ ++ F+T E +L+QKG HK+QREEL+A+L++FEKIL  LPE + ++WQ+HS+G ++ KLLH GNS K ++RE +RLF+LW Q L +        MF+ L+PGF +P    G   L  +     + Q+  V   EI PL+PPQSG++ +     DLT  FLE LL+ +V QV  +EWK NK E Q+R   FLF  FK YY+P+IFP      SLY+P L++P++R  +P ++      +T +      +P +  RV+VI+W+  +
Sbjct:    1 MFSKKPH-----GDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLKQKG-HKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLK-IRREGVRLFLLWLQALQNNCSREQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQ----EDLTSYFLEALLKYIVIQVKSLEWK-NK-ENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPVLDIPQMRP-KPHYVMIKKDAETNEAIYCTKEPFIKARVIVIRWLVSF 312          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|74855334|sp|Q54TK4.1|Y1809_DICDI (RecName: Full=Rap-GAP domain-containing protein DDB_G0281809)

HSP 1 Score: 134.806 bits (338), Expect = 3.738e-30
Identity = 90/307 (29.32%), Postives = 157/307 (51.14%), Query Frame = 0

Query: 1557 YSAHPEV---PPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASG--SQAYEEFVSGLAWEVELETHTGFMGGLQKSKTT-GETAPYYATSFLEVMFHVATRMPS---LSEESMLQKTRHIGNDEIHIVW-SEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVP---YFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTF---YQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLFQ 1847
            YS  P +   PP L+ + +     QQ R L +HLG  + E R  +  ++ + +  + L  LD+   R   KI +++V +G   +  I +N +   +Q Y++F++ L W V + THTGF+G L K   T G+  PYYAT   E++F V+T MP+    +  S   K + I    + ++W +     Y + +L T    + IVI PL+N LFR++  RK         GP+ +E+I+ + +L  +IR + +N++++  ++ +     Q  Y  R K + +I  + K   T ++F    F P   + L+Q
Sbjct: 1216 YSLTPSITTHPPVLKTSFNGDCKLQQARILLTHLGFLNQENRNKLTPLENSVQFFQSLNMLDSVSERVQIKIPIIYVKKGDSTEDDIYNNVTSNTTQDYQDFIASLGWLVPISTHTGFLGDLDKKNLTHGQFTPYYATHSREMVFFVSTMMPNSDIANNSSQEHKKKLINKTNVSVIWFNGSIEVYEKTLLETFPHAIQIVITPLENDLFRLKTLRKATHSNRMKTGPVNDEIIISKHILANVIRLSVVNSNQSLLNLSSGDGKTQHIYTNRKKLISDITESFKHDMTIQKFYEFHFEPLDQSQLYQ 1522          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|1717800|sp|P49816.1|TSC2_RAT (RecName: Full=Tuberin; AltName: Full=Tuberous sclerosis 2 protein homolog)

HSP 1 Score: 97.4413 bits (241), Expect = 9.815e-19
Identity = 62/208 (29.81%), Postives = 108/208 (51.92%), Query Frame = 0

Query: 1597 NVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQ-SILSNASGSQAYEEFVSGLAWEVEL---ETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLS-EESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKR 1799
            N  ++  NE   R ++ LD     + HKIAV++VGEGQ   + +ILSN  GS  Y EF++GL   +EL   +    ++GGL      G+    +    ++ +FH+AT MP+   ++    K RH+GND + I++++   D++ G +  +F  V ++I PL  K   + +  + ++          IV  R L  + R  A++A+ A +
Sbjct: 1519 NKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQ 1726          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971379|ref|XP_021917095.1| (ral GTPase-activating protein subunit alpha-1 isoform X5 [Zootermopsis nevadensis] >gi|646718494|gb|KDR20934.1| 250 kDa substrate of Akt [Zootermopsis nevadensis])

HSP 1 Score: 1868.97 bits (4840), Expect = 0.000e+0
Identity = 1023/1954 (52.35%), Postives = 1306/1954 (66.84%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKGAHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTAL----AAMTPDTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKMDPMLSCRVVVIKWVAQYTHITRSGAMGSSGTLAASNLGLNGTMD-----------VLQSGLN----LSMSEKDTSFQVEHM-----SLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKERDELDYAMTGKLSADMADPNDSGY-------RLRNDSYLGAIHKEHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRG--QRGGPQDLKYSS------------------SSIKQPVTP---VSEVHPPILDDSSGAGSLGGAPQTQFRRQGSPYPR----RKRISSETEYKSSPNERRQHQH----------VRFYPTNLRRSMSEGYIKQPPTQLIGEYNFHLESKRRHRSVESMYTYSR------SESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1846
            MFSKK +      DVKKS  K+ D K+D+ TR KHL+I L+N D  E    FEA +SHI+Y++YD F+TAE NLRQ+  HKA REELE VL + EK+L+YLPE L R+WQ HS+ R+M+KLLHPGNSWKL +REA+R F++WYQ LGD A E LHA+FATLVPGFPSP+ G GL  L     ++  DT  GPVN  EI PL+PPQSGE+    PD D  R FL+ +LE +VSQV  +EW++    RQ +C  FLF++FK YY+PHIFP+F++  SLY P L+LP +RK  P   DT +G ++ DP + CRVVVIKWVA +THIT+ G      T + S++  +   D           V Q+G N    L  S+ + S    H      SLS  ED   N+   ++VR VLY+NR+NV+FVHEVYRQAFLL+F+HSPAI+K I VYKDWIQMNV ELP F+LEPL   KE    DY  +G  S + +    S         RLRNDSYLGAIH+E+L VRAGLQNVLQ+FIT+AANVF+LE+SPEYP+LLEEQV+ CKRVLNIYRYMVM+ +M++RTWEQLL +LLQIT L + ++PP+R+E+TLGG+LA AIFQTLIVTWIKANL VV+S ELWDQFL VLSSLT WEELIREWAKT+ETLTRVLAR VY LDL DLPLDRL+E+ AKKRRG  Q+ G  D   +S                  S  + PV P    S    P  D+  G+  + G+P+ +    G    R    R    S   Y      R++  H          +   P ++ + ++   +  P ++ +  Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A          +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:    1 MFSKKLH-----ADVKKSTLKIQDLKKDSATRLKHLKIILENVDAEEAKSIFEANFSHIYYVLYDTFVTAETNLRQR-VHKAHREELECVLQVLEKVLVYLPELLSRRWQCHSLTRIMTKLLHPGNSWKL-RREAIRYFLMWYQALGDNAPETLHAIFATLVPGFPSPFPGQGLCTLNRSSTSVFHDTSHGPVNAVEIQPLLPPQSGEK---QPD-DPMRFFLDSVLEFIVSQVTKVEWRDRLC-RQHKCFMFLFERFKQYYLPHIFPQFSFATSLYKPVLDLPVLRKGSPGPSDTPEGRRRQDPFVICRVVVIKWVATFTHITKKGDTPFMHTQSNSSVTPSEDADHSPGTELRRVSVSQAGSNHDATLPASDSNHSSVFSHTEGGNHSLS--EDP--NAAAVHLVREVLYSNRDNVNFVHEVYRQAFLLNFTHSPAIRKAIAVYKDWIQMNVPELPSFMLEPLESHKE----DYYRSGPPSFEYSSMEGSETSESYRVTRLRNDSYLGAIHRENLLVRAGLQNVLQVFITHAANVFMLEVSPEYPLLLEEQVDTCKRVLNIYRYMVMHTRMESRTWEQLLLVLLQITSLILSKTPPKRKEETLGGKLAPAIFQTLIVTWIKANLNVVISAELWDQFLMVLSSLTQWEELIREWAKTLETLTRVLARHVYNLDLTDLPLDRLTEQKAKKRRGGKQQAGNGDQSRASFSSAGRRGSEMSSQGKTLSQSQCPVQPEDGCSSYTSP--DNGLGSLRIHGSPRKRRDSSGDSSQRLHLPRSVSESNLTYGRGGAPRKRGIHTSRPQSNTVVIPVLPISVEQEVAR-LMASPESRSVN-YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMT---TFPHQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1881          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971375|ref|XP_021917093.1| (ral GTPase-activating protein subunit alpha-1 isoform X3 [Zootermopsis nevadensis])

HSP 1 Score: 1865.89 bits (4832), Expect = 0.000e+0
Identity = 1023/1959 (52.22%), Postives = 1306/1959 (66.67%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKG-----AHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTAL----AAMTPDTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKMDPMLSCRVVVIKWVAQYTHITRSGAMGSSGTLAASNLGLNGTMD-----------VLQSGLN----LSMSEKDTSFQVEHM-----SLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKERDELDYAMTGKLSADMADPNDSGY-------RLRNDSYLGAIHKEHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRG--QRGGPQDLKYSS------------------SSIKQPVTP---VSEVHPPILDDSSGAGSLGGAPQTQFRRQGSPYPR----RKRISSETEYKSSPNERRQHQH----------VRFYPTNLRRSMSEGYIKQPPTQLIGEYNFHLESKRRHRSVESMYTYSR------SESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1846
            MFSKK +      DVKKS  K+ D K+D+ TR KHL+I L+N D  E    FEA +SHI+Y++YD F+TAE NLRQ+       HKA REELE VL + EK+L+YLPE L R+WQ HS+ R+M+KLLHPGNSWKL +REA+R F++WYQ LGD A E LHA+FATLVPGFPSP+ G GL  L     ++  DT  GPVN  EI PL+PPQSGE+    PD D  R FL+ +LE +VSQV  +EW++    RQ +C  FLF++FK YY+PHIFP+F++  SLY P L+LP +RK  P   DT +G ++ DP + CRVVVIKWVA +THIT+ G      T + S++  +   D           V Q+G N    L  S+ + S    H      SLS  ED   N+   ++VR VLY+NR+NV+FVHEVYRQAFLL+F+HSPAI+K I VYKDWIQMNV ELP F+LEPL   KE    DY  +G  S + +    S         RLRNDSYLGAIH+E+L VRAGLQNVLQ+FIT+AANVF+LE+SPEYP+LLEEQV+ CKRVLNIYRYMVM+ +M++RTWEQLL +LLQIT L + ++PP+R+E+TLGG+LA AIFQTLIVTWIKANL VV+S ELWDQFL VLSSLT WEELIREWAKT+ETLTRVLAR VY LDL DLPLDRL+E+ AKKRRG  Q+ G  D   +S                  S  + PV P    S    P  D+  G+  + G+P+ +    G    R    R    S   Y      R++  H          +   P ++ + ++   +  P ++ +  Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A          +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:    1 MFSKKLH-----ADVKKSTLKIQDLKKDSATRLKHLKIILENVDAEEAKSIFEANFSHIYYVLYDTFVTAETNLRQRELSFHLVHKAHREELECVLQVLEKVLVYLPELLSRRWQCHSLTRIMTKLLHPGNSWKL-RREAIRYFLMWYQALGDNAPETLHAIFATLVPGFPSPFPGQGLCTLNRSSTSVFHDTSHGPVNAVEIQPLLPPQSGEK---QPD-DPMRFFLDSVLEFIVSQVTKVEWRDRLC-RQHKCFMFLFERFKQYYLPHIFPQFSFATSLYKPVLDLPVLRKGSPGPSDTPEGRRRQDPFVICRVVVIKWVATFTHITKKGDTPFMHTQSNSSVTPSEDADHSPGTELRRVSVSQAGSNHDATLPASDSNHSSVFSHTEGGNHSLS--EDP--NAAAVHLVREVLYSNRDNVNFVHEVYRQAFLLNFTHSPAIRKAIAVYKDWIQMNVPELPSFMLEPLESHKE----DYYRSGPPSFEYSSMEGSETSESYRVTRLRNDSYLGAIHRENLLVRAGLQNVLQVFITHAANVFMLEVSPEYPLLLEEQVDTCKRVLNIYRYMVMHTRMESRTWEQLLLVLLQITSLILSKTPPKRKEETLGGKLAPAIFQTLIVTWIKANLNVVISAELWDQFLMVLSSLTQWEELIREWAKTLETLTRVLARHVYNLDLTDLPLDRLTEQKAKKRRGGKQQAGNGDQSRASFSSAGRRGSEMSSQGKTLSQSQCPVQPEDGCSSYTSP--DNGLGSLRIHGSPRKRRDSSGDSSQRLHLPRSVSESNLTYGRGGAPRKRGIHTSRPQSNTVVIPVLPISVEQEVAR-LMASPESRSVN-YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMT---TFPHQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1887          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971377|ref|XP_021917094.1| (ral GTPase-activating protein subunit alpha-1 isoform X4 [Zootermopsis nevadensis])

HSP 1 Score: 1863.2 bits (4825), Expect = 0.000e+0
Identity = 1023/1957 (52.27%), Postives = 1306/1957 (66.73%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKGAHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTAL----AAMTPDTQD---GPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKMDPMLSCRVVVIKWVAQYTHITRSGAMGSSGTLAASNLGLNGTMD-----------VLQSGLN----LSMSEKDTSFQVEHM-----SLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKERDELDYAMTGKLSADMADPNDSGY-------RLRNDSYLGAIHKEHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRG--QRGGPQDLKYSS------------------SSIKQPVTP---VSEVHPPILDDSSGAGSLGGAPQTQFRRQGSPYPR----RKRISSETEYKSSPNERRQHQH----------VRFYPTNLRRSMSEGYIKQPPTQLIGEYNFHLESKRRHRSVESMYTYSR------SESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1846
            MFSKK +      DVKKS  K+ D K+D+ TR KHL+I L+N D  E    FEA +SHI+Y++YD F+TAE NLRQ+  HKA REELE VL + EK+L+YLPE L R+WQ HS+ R+M+KLLHPGNSWKL +REA+R F++WYQ LGD A E LHA+FATLVPGFPSP+ G GL  L     ++  DT     GPVN  EI PL+PPQSGE+    PD D  R FL+ +LE +VSQV  +EW++    RQ +C  FLF++FK YY+PHIFP+F++  SLY P L+LP +RK  P   DT +G ++ DP + CRVVVIKWVA +THIT+ G      T + S++  +   D           V Q+G N    L  S+ + S    H      SLS  ED   N+   ++VR VLY+NR+NV+FVHEVYRQAFLL+F+HSPAI+K I VYKDWIQMNV ELP F+LEPL   KE    DY  +G  S + +    S         RLRNDSYLGAIH+E+L VRAGLQNVLQ+FIT+AANVF+LE+SPEYP+LLEEQV+ CKRVLNIYRYMVM+ +M++RTWEQLL +LLQIT L + ++PP+R+E+TLGG+LA AIFQTLIVTWIKANL VV+S ELWDQFL VLSSLT WEELIREWAKT+ETLTRVLAR VY LDL DLPLDRL+E+ AKKRRG  Q+ G  D   +S                  S  + PV P    S    P  D+  G+  + G+P+ +    G    R    R    S   Y      R++  H          +   P ++ + ++   +  P ++ +  Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A          +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:    1 MFSKKLH-----ADVKKSTLKIQDLKKDSATRLKHLKIILENVDAEEAKSIFEANFSHIYYVLYDTFVTAETNLRQR-VHKAHREELECVLQVLEKVLVYLPELLSRRWQCHSLTRIMTKLLHPGNSWKL-RREAIRYFLMWYQALGDNAPETLHAIFATLVPGFPSPFPGQGLCTLNRSSTSVFHDTSHVTAGPVNAVEIQPLLPPQSGEK---QPD-DPMRFFLDSVLEFIVSQVTKVEWRDRLC-RQHKCFMFLFERFKQYYLPHIFPQFSFATSLYKPVLDLPVLRKGSPGPSDTPEGRRRQDPFVICRVVVIKWVATFTHITKKGDTPFMHTQSNSSVTPSEDADHSPGTELRRVSVSQAGSNHDATLPASDSNHSSVFSHTEGGNHSLS--EDP--NAAAVHLVREVLYSNRDNVNFVHEVYRQAFLLNFTHSPAIRKAIAVYKDWIQMNVPELPSFMLEPLESHKE----DYYRSGPPSFEYSSMEGSETSESYRVTRLRNDSYLGAIHRENLLVRAGLQNVLQVFITHAANVFMLEVSPEYPLLLEEQVDTCKRVLNIYRYMVMHTRMESRTWEQLLLVLLQITSLILSKTPPKRKEETLGGKLAPAIFQTLIVTWIKANLNVVISAELWDQFLMVLSSLTQWEELIREWAKTLETLTRVLARHVYNLDLTDLPLDRLTEQKAKKRRGGKQQAGNGDQSRASFSSAGRRGSEMSSQGKTLSQSQCPVQPEDGCSSYTSP--DNGLGSLRIHGSPRKRRDSSGDSSQRLHLPRSVSESNLTYGRGGAPRKRGIHTSRPQSNTVVIPVLPISVEQEVAR-LMASPESRSVN-YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMT---TFPHQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1884          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971371|ref|XP_021917090.1| (ral GTPase-activating protein subunit alpha-1 isoform X1 [Zootermopsis nevadensis])

HSP 1 Score: 1861.27 bits (4820), Expect = 0.000e+0
Identity = 1023/1962 (52.14%), Postives = 1306/1962 (66.56%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKG-----AHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTAL----AAMTPDTQD---GPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKMDPMLSCRVVVIKWVAQYTHITRSGAMGSSGTLAASNLGLNGTMD-----------VLQSGLN----LSMSEKDTSFQVEHM-----SLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKERDELDYAMTGKLSADMADPNDSGY-------RLRNDSYLGAIHKEHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRG--QRGGPQDLKYSS------------------SSIKQPVTP---VSEVHPPILDDSSGAGSLGGAPQTQFRRQGSPYPR----RKRISSETEYKSSPNERRQHQH----------VRFYPTNLRRSMSEGYIKQPPTQLIGEYNFHLESKRRHRSVESMYTYSR------SESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1846
            MFSKK +      DVKKS  K+ D K+D+ TR KHL+I L+N D  E    FEA +SHI+Y++YD F+TAE NLRQ+       HKA REELE VL + EK+L+YLPE L R+WQ HS+ R+M+KLLHPGNSWKL +REA+R F++WYQ LGD A E LHA+FATLVPGFPSP+ G GL  L     ++  DT     GPVN  EI PL+PPQSGE+    PD D  R FL+ +LE +VSQV  +EW++    RQ +C  FLF++FK YY+PHIFP+F++  SLY P L+LP +RK  P   DT +G ++ DP + CRVVVIKWVA +THIT+ G      T + S++  +   D           V Q+G N    L  S+ + S    H      SLS  ED   N+   ++VR VLY+NR+NV+FVHEVYRQAFLL+F+HSPAI+K I VYKDWIQMNV ELP F+LEPL   KE    DY  +G  S + +    S         RLRNDSYLGAIH+E+L VRAGLQNVLQ+FIT+AANVF+LE+SPEYP+LLEEQV+ CKRVLNIYRYMVM+ +M++RTWEQLL +LLQIT L + ++PP+R+E+TLGG+LA AIFQTLIVTWIKANL VV+S ELWDQFL VLSSLT WEELIREWAKT+ETLTRVLAR VY LDL DLPLDRL+E+ AKKRRG  Q+ G  D   +S                  S  + PV P    S    P  D+  G+  + G+P+ +    G    R    R    S   Y      R++  H          +   P ++ + ++   +  P ++ +  Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A          +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:    1 MFSKKLH-----ADVKKSTLKIQDLKKDSATRLKHLKIILENVDAEEAKSIFEANFSHIYYVLYDTFVTAETNLRQRELSFHLVHKAHREELECVLQVLEKVLVYLPELLSRRWQCHSLTRIMTKLLHPGNSWKL-RREAIRYFLMWYQALGDNAPETLHAIFATLVPGFPSPFPGQGLCTLNRSSTSVFHDTSHVTAGPVNAVEIQPLLPPQSGEK---QPD-DPMRFFLDSVLEFIVSQVTKVEWRDRLC-RQHKCFMFLFERFKQYYLPHIFPQFSFATSLYKPVLDLPVLRKGSPGPSDTPEGRRRQDPFVICRVVVIKWVATFTHITKKGDTPFMHTQSNSSVTPSEDADHSPGTELRRVSVSQAGSNHDATLPASDSNHSSVFSHTEGGNHSLS--EDP--NAAAVHLVREVLYSNRDNVNFVHEVYRQAFLLNFTHSPAIRKAIAVYKDWIQMNVPELPSFMLEPLESHKE----DYYRSGPPSFEYSSMEGSETSESYRVTRLRNDSYLGAIHRENLLVRAGLQNVLQVFITHAANVFMLEVSPEYPLLLEEQVDTCKRVLNIYRYMVMHTRMESRTWEQLLLVLLQITSLILSKTPPKRKEETLGGKLAPAIFQTLIVTWIKANLNVVISAELWDQFLMVLSSLTQWEELIREWAKTLETLTRVLARHVYNLDLTDLPLDRLTEQKAKKRRGGKQQAGNGDQSRASFSSAGRRGSEMSSQGKTLSQSQCPVQPEDGCSSYTSP--DNGLGSLRIHGSPRKRRDSSGDSSQRLHLPRSVSESNLTYGRGGAPRKRGIHTSRPQSNTVVIPVLPISVEQEVAR-LMASPESRSVN-YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMT---TFPHQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1890          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971373|ref|XP_021917091.1| (ral GTPase-activating protein subunit alpha-1 isoform X2 [Zootermopsis nevadensis])

HSP 1 Score: 1860.5 bits (4818), Expect = 0.000e+0
Identity = 1020/1963 (51.96%), Postives = 1300/1963 (66.23%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKG-----AHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTAL----AAMTPDTQD---GPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKMDPMLSCRVVVIKWVAQYTHITRSG-----------------AMGSSGT----LAASNLGLNGTMDVLQSGLNLSMSEKDTSFQVEHMSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKERDELDYAMTGKLSADMADPNDSGY-------RLRNDSYLGAIHKEHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRG--QRGGPQDLKYSS------------------SSIKQPVTP---VSEVHPPILDDSSGAGSLGGAPQTQFRRQGSPYPR----RKRISSETEYKSSPNERRQHQH----------VRFYPTNLRRSMSEGYIKQPPTQLIGEYNFHLESKRRHRSVESMYTYSR------SESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1846
            MFSKK +      DVKKS  K+ D K+D+ TR KHL+I L+N D  E    FEA +SHI+Y++YD F+TAE NLRQ+       HKA REELE VL + EK+L+YLPE L R+WQ HS+ R+M+KLLHPGNSWKL +REA+R F++WYQ LGD A E LHA+FATLVPGFPSP+ G GL  L     ++  DT     GPVN  EI PL+PPQSGE+    PD D  R FL+ +LE +VSQV  +EW++    RQ +C  FLF++FK YY+PHIFP+F++  SLY P L+LP +RK  P   DT +G ++ DP + CRVVVIKWVA +THIT+ G                 A  S GT    ++ S  G N    +  S  N S     T      +S  P      N+   ++VR VLY+NR+NV+FVHEVYRQAFLL+F+HSPAI+K I VYKDWIQMNV ELP F+LEPL   KE    DY  +G  S + +    S         RLRNDSYLGAIH+E+L VRAGLQNVLQ+FIT+AANVF+LE+SPEYP+LLEEQV+ CKRVLNIYRYMVM+ +M++RTWEQLL +LLQIT L + ++PP+R+E+TLGG+LA AIFQTLIVTWIKANL VV+S ELWDQFL VLSSLT WEELIREWAKT+ETLTRVLAR VY LDL DLPLDRL+E+ AKKRRG  Q+ G  D   +S                  S  + PV P    S    P  D+  G+  + G+P+ +    G    R    R    S   Y      R++  H          +   P ++ + ++   +  P ++ +  Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A          +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:    1 MFSKKLH-----ADVKKSTLKIQDLKKDSATRLKHLKIILENVDAEEAKSIFEANFSHIYYVLYDTFVTAETNLRQRELSFHLVHKAHREELECVLQVLEKVLVYLPELLSRRWQCHSLTRIMTKLLHPGNSWKL-RREAIRYFLMWYQALGDNAPETLHAIFATLVPGFPSPFPGQGLCTLNRSSTSVFHDTSHVTAGPVNAVEIQPLLPPQSGEK---QPD-DPMRFFLDSVLEFIVSQVTKVEWRDRLC-RQHKCFMFLFERFKQYYLPHIFPQFSFATSLYKPVLDLPVLRKGSPGPSDTPEGRRRQDPFVICRVVVIKWVATFTHITKKGDTPFMHTQSNSVTPSEDADHSPGTELRRVSVSQAGSNHDATLPASDSNHSSVFSHTEGGNHSLSEDP------NAAAVHLVREVLYSNRDNVNFVHEVYRQAFLLNFTHSPAIRKAIAVYKDWIQMNVPELPSFMLEPLESHKE----DYYRSGPPSFEYSSMEGSETSESYRVTRLRNDSYLGAIHRENLLVRAGLQNVLQVFITHAANVFMLEVSPEYPLLLEEQVDTCKRVLNIYRYMVMHTRMESRTWEQLLLVLLQITSLILSKTPPKRKEETLGGKLAPAIFQTLIVTWIKANLNVVISAELWDQFLMVLSSLTQWEELIREWAKTLETLTRVLARHVYNLDLTDLPLDRLTEQKAKKRRGGKQQAGNGDQSRASFSSAGRRGSEMSSQGKTLSQSQCPVQPEDGCSSYTSP--DNGLGSLRIHGSPRKRRDSSGDSSQRLHLPRSVSESNLTYGRGGAPRKRGIHTSRPQSNTVVIPVLPISVEQEVAR-LMASPESRSVN-YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMT---TFPHQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1889          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971381|ref|XP_021917096.1| (ral GTPase-activating protein subunit alpha-1 isoform X6 [Zootermopsis nevadensis])

HSP 1 Score: 1859.34 bits (4815), Expect = 0.000e+0
Identity = 1022/1962 (52.09%), Postives = 1305/1962 (66.51%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKG-----AHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGL-------TALAAMTPDTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKMDPMLSCRVVVIKWVAQYTHITRSGAMGSSGTLAASNLGLNGTMD-----------VLQSGLN----LSMSEKDTSFQVEHM-----SLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKERDELDYAMTGKLSADMADPNDSGY-------RLRNDSYLGAIHKEHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRG--QRGGPQDLKYSS------------------SSIKQPVTP---VSEVHPPILDDSSGAGSLGGAPQTQFRRQGSPYPR----RKRISSETEYKSSPNERRQHQH----------VRFYPTNLRRSMSEGYIKQPPTQLIGEYNFHLESKRRHRSVESMYTYSR------SESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1846
            MFSKK +      DVKKS  K+ D K+D+ TR KHL+I L+N D  E    FEA +SHI+Y++YD F+TAE NLRQ+       HKA REELE VL + EK+L+YLPE L R+WQ HS+ R+M+KLLHPGNSWKL +REA+R F++WYQ LGD A E LHA+FATLVPGFPSP+ G GL       T++   T     GPVN  EI PL+PPQSGE+    PD D  R FL+ +LE +VSQV  +EW++    RQ +C  FLF++FK YY+PHIFP+F++  SLY P L+LP +RK  P   DT +G ++ DP + CRVVVIKWVA +THIT+ G      T + S++  +   D           V Q+G N    L  S+ + S    H      SLS  ED   N+   ++VR VLY+NR+NV+FVHEVYRQAFLL+F+HSPAI+K I VYKDWIQMNV ELP F+LEPL   KE    DY  +G  S + +    S         RLRNDSYLGAIH+E+L VRAGLQNVLQ+FIT+AANVF+LE+SPEYP+LLEEQV+ CKRVLNIYRYMVM+ +M++RTWEQLL +LLQIT L + ++PP+R+E+TLGG+LA AIFQTLIVTWIKANL VV+S ELWDQFL VLSSLT WEELIREWAKT+ETLTRVLAR VY LDL DLPLDRL+E+ AKKRRG  Q+ G  D   +S                  S  + PV P    S    P  D+  G+  + G+P+ +    G    R    R    S   Y      R++  H          +   P ++ + ++   +  P ++ +  Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A          +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:    1 MFSKKLH-----ADVKKSTLKIQDLKKDSATRLKHLKIILENVDAEEAKSIFEANFSHIYYVLYDTFVTAETNLRQRELSFHLVHKAHREELECVLQVLEKVLVYLPELLSRRWQCHSLTRIMTKLLHPGNSWKL-RREAIRYFLMWYQALGDNAPETLHAIFATLVPGFPSPFPGQGLCTLNRSSTSVFHDTSHVTAGPVNAVEIQPLLPPQSGEK---QPD-DPMRFFLDSVLEFIVSQVTKVEWRDRLC-RQHKCFMFLFERFKQYYLPHIFPQFSFATSLYKPVLDLPVLRKGSPGPSDTPEGRRRQDPFVICRVVVIKWVATFTHITKKGDTPFMHTQSNSSVTPSEDADHSPGTELRRVSVSQAGSNHDATLPASDSNHSSVFSHTEGGNHSLS--EDP--NAAAVHLVREVLYSNRDNVNFVHEVYRQAFLLNFTHSPAIRKAIAVYKDWIQMNVPELPSFMLEPLESHKE----DYYRSGPPSFEYSSMEGSETSESYRVTRLRNDSYLGAIHRENLLVRAGLQNVLQVFITHAANVFMLEVSPEYPLLLEEQVDTCKRVLNIYRYMVMHTRMESRTWEQLLLVLLQITSLILSKTPPKRKEETLGGKLAPAIFQTLIVTWIKANLNVVISAELWDQFLMVLSSLTQWEELIREWAKTLETLTRVLARHVYNLDLTDLPLDRLTEQKAKKRRGGKQQAGNGDQSRASFSSAGRRGSEMSSQGKTLSQSQCPVQPEDGCSSYTSP--DNGLGSLRIHGSPRKRRDSSGDSSQRLHLPRSVSESNLTYGRGGAPRKRGIHTSRPQSNTVVIPVLPISVEQEVAR-LMASPESRSVN-YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMT---TFPHQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1890          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1339045765|ref|XP_023711025.1| (probable Rho GTPase-activating protein CG5521 isoform X3 [Cryptotermes secundus] >gi|1330907060|gb|PNF30190.1| Ral GTPase-activating protein subunit alpha-2 [Cryptotermes secundus])

HSP 1 Score: 1849.71 bits (4790), Expect = 0.000e+0
Identity = 1022/1942 (52.63%), Postives = 1305/1942 (67.20%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKGAHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTAL----AAMTPDTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKMDPMLSCRVVVIKWVAQYTHITRSGAMGSSGTLAASNLGLNGTMD-----------VLQSGLN------LSMSEKDTSF-QVEHMSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKE---RDELDYAMTGKLSADMADPNDSGYRLRNDSYLGAIHKEHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRG--QRGGPQ-DLKYSS-----SSIKQPVTPVSEVHPPILDDSSGAGSLGGAPQTQ---FRRQGSPYPRRK------------RISSETE--YKSSPNERRQHQHVRFYPTNLRRSMSEGYIKQPPTQLIG---EYNFHLESK----RRHRSVESMYTYSR------SESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIP-HFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSW----------DSCAMNGLTK---SPNGIIEMDYSK-------LAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN--AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA-----HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1846
            MFSKK +      DVKKS  K+ D K+D+ TR KHL+I L+N D  E    FE+ +SHI+YI+YD F+TAE NLRQ+  HKA REELE VL + EK+L+YLPE L R+WQ HS+ R+M+KLLHPGNSWKL +REA+R F+LWYQ L D + E LHA+FATLVPGFPSP+ G GL+AL     ++  D   GPVN  EI PL+PPQSGE+    PD D  R FL+ LLE +VSQV  IEW++    +Q +C  FLF++FK YY+PHIFP+F++  SLY P L+LP +RK  P   DT +G ++ DP + CRVVVIKWVA +THIT+ G +    T + S++  +   D           V Q+G N       S S   + F Q E  + S  ED   N+   ++V  VLY++RENV+FVHEVYRQAFLL+F+HSPAI+K I VYKDWIQMNV ELP F+LEPL   KE   R          + +  A  +    RLRNDSYLGAIHKE+L VRAGLQNVLQ+FIT+AANVF+LE+SPEYP+LLEEQV+ CKRVLNIYRYMVM+ +M++RTWEQLL +LLQIT L + ++PP+R+E+TLGG+LA AIFQTLIVTWIKANL V++SPELWD+FL VLSSLT WEELIREWAKT+ETLTRVLAR VY LDLNDLPLDRL+E+ AKKRRG  Q+GG Q    +SS     S I      +S+ H P         S   +P+      R  GSP  RR             R  SE+   Y      R+ +   R     +   +    ++Q   +L+      + H + +    R+ RSV+S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + +  +H+Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PP  I APWC KAL LP +YQ+GKL A RLLC +T  P D  L + HL QFYKVLH+GL G+   V+NTL++++GPRFFSLMLPG++  + DF+ AAN  +S+S+L+G PRTEA S++GALL  P+   +  +L PN+ E +++  +D KD I+ +LLK GK+EPAG ARCI+LSSLGI++Y EL H  FHPK KEA+ VLL ALRFNNK V Q+ASD+LLLL DH    ++ YPE+P +I+EVLA TL  L P+     +E +K LL SL+FCLGEWCM+IP +++L     +G  LL  VF+ L Q                        +  G   +   +  P  +V DFDPNI +D+ KEG     ++P    +S +  QE+ S+         SN  +++AA+ V+ HLVNHL HFPM  GAA LSS+V E DD+PGL TDEL  +VF  PNIQLF+L+N+ ++SLVELP +E         ++T+  QVR+ILRD+SGK SW          DS   + LTK   + +G+     SK       L + +L SS  G +  S      +R R   +LPT +N AEDMDNLDDLLQY+ +TSPEC E +  P N  A P   L   E E D I+ V++QR  EQ+++++ + ++T  A      P  P Q        S FQ CR LFS LGL+ WE+R  +HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+R+QVSRK EVPYFGPLFNE IV  +VLPGL+RA+AI ASRAKRSML FYQ++YEER+K+LE ++ NHKD TTFEEF  QV+SP   T+ F
Sbjct:    1 MFSKKLH-----ADVKKSTLKIQDLKKDSATRLKHLKIILENVDAEEAKSVFESNFSHIYYILYDTFVTAEANLRQR-VHKAHREELECVLQVLEKVLVYLPELLSRRWQCHSLTRIMTKLLHPGNSWKL-RREAIRYFLLWYQSLADNSPEALHAIFATLVPGFPSPFAGQGLSALNRSSTSVFHDISRGPVNAVEIQPLLPPQSGEK---QPD-DPMRYFLDALLEFIVSQVTKIEWRDRLC-KQHKCFVFLFERFKQYYLPHIFPQFSFATSLYKPVLDLPVLRKGSPGLSDTPEGRRRQDPFVVCRVVVIKWVATFTHITKKGEVPFMHTQSNSSVTPSEDTDHSPGTELRRVSVSQAGSNHDAASPASDSNHSSVFSQSEGGNHSLSEDP--NAAAMHLVCEVLYSSRENVNFVHEVYRQAFLLNFTHSPAIRKAIAVYKDWIQMNVPELPSFMLEPLESHKEDIYRSGPPSYEYSSMESSEAAESYRVTRLRNDSYLGAIHKENLLVRAGLQNVLQVFITHAANVFMLEVSPEYPLLLEEQVDTCKRVLNIYRYMVMHTRMESRTWEQLLMVLLQITSLILSKTPPKRKEETLGGKLAPAIFQTLIVTWIKANLNVIISPELWDRFLVVLSSLTQWEELIREWAKTLETLTRVLARHVYNLDLNDLPLDRLTEQKAKKRRGGKQQGGDQPQASFSSTGRRGSEISSQGKTLSQSHGP--SQPEDGLSSYTSPEIGLGLLRLHGSPGKRRDSSGDSSQRSHLPRSVSESNLMYGQCTPSRKMYHTSRPQNNTVVIPVLPISVEQEVARLMASPQSSSIHCQPRNRLCRKSRSVDSLRGARSGTPNQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLSGGDGTSLD---LPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLTNPALHSQVFEYLVELCDTLAKIRLNQGVSDDNQVTPPPPDLIPPLNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISLPRDHLSQFYKVLHQGLVGTDQAVINTLVQFSGPRFFSLMLPGHSLLLYDFIHAANTIVSSSDLRGTPRTEAVSILGALLCLPNTFAETLVLQPNAGEFTMMPCSDAKDHILSILLKCGKREPAGQARCIALSSLGIYLYQELTHEIFHPKNKEAMNVLLLALRFNNKTVGQVASDILLLLCDHADRLVEHYPEIPSRIIEVLALTLSHLMPQNNVTTSERDKHLLTSLMFCLGEWCMKIPANYLLA--SNNGSCLLLTVFKVLNQL------------------QGGGGSGSGVSGSTGGLFQPD-LVQDFDPNILLDDLKEG-----STPPVGRRSSAMIQESGSLSHHHH----SNQSIQMAARMVMMHLVNHLGHFPMGIGAARLSSMVVEHDDVPGLATDELSAEVFSAPNIQLFVLSNSLMVSLVELPALEVPGGGITAGLRTAPSQVRIILRDLSGKASWDASILYCSPEDSICSHRLTKEEETEDGVGICGSSKPISVSSRLEEAMLTSSFVGGELHSCPPQHTLRHRPPHMLPTCDNGAEDMDNLDDLLQYLGYTSPECLESLDTPRNIPATPPPPL-TREAEEDTITMVLNQRNAEQEYLQRRSADITMLAGCMATFPHKPSQ--------SPFQHCRLLFSQLGLAGWERRSQLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKSSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRVQVSRKSEVPYFGPLFNESIVDDKVLPGLVRASAIGASRAKRSMLPFYQNYYEERAKSLETVVRNHKDATTFEEFTAQVYSPVQATSPF 1880          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1339045761|ref|XP_023711023.1| (probable Rho GTPase-activating protein CG5521 isoform X1 [Cryptotermes secundus])

HSP 1 Score: 1847.79 bits (4785), Expect = 0.000e+0
Identity = 1022/1947 (52.49%), Postives = 1305/1947 (67.03%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKG-----AHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTAL----AAMTPDTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKMDPMLSCRVVVIKWVAQYTHITRSGAMGSSGTLAASNLGLNGTMD-----------VLQSGLN------LSMSEKDTSF-QVEHMSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKE---RDELDYAMTGKLSADMADPNDSGYRLRNDSYLGAIHKEHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRG--QRGGPQ-DLKYSS-----SSIKQPVTPVSEVHPPILDDSSGAGSLGGAPQTQ---FRRQGSPYPRRK------------RISSETE--YKSSPNERRQHQHVRFYPTNLRRSMSEGYIKQPPTQLIG---EYNFHLESK----RRHRSVESMYTYSR------SESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIP-HFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSW----------DSCAMNGLTK---SPNGIIEMDYSK-------LAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN--AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA-----HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1846
            MFSKK +      DVKKS  K+ D K+D+ TR KHL+I L+N D  E    FE+ +SHI+YI+YD F+TAE NLRQ+       HKA REELE VL + EK+L+YLPE L R+WQ HS+ R+M+KLLHPGNSWKL +REA+R F+LWYQ L D + E LHA+FATLVPGFPSP+ G GL+AL     ++  D   GPVN  EI PL+PPQSGE+    PD D  R FL+ LLE +VSQV  IEW++    +Q +C  FLF++FK YY+PHIFP+F++  SLY P L+LP +RK  P   DT +G ++ DP + CRVVVIKWVA +THIT+ G +    T + S++  +   D           V Q+G N       S S   + F Q E  + S  ED   N+   ++V  VLY++RENV+FVHEVYRQAFLL+F+HSPAI+K I VYKDWIQMNV ELP F+LEPL   KE   R          + +  A  +    RLRNDSYLGAIHKE+L VRAGLQNVLQ+FIT+AANVF+LE+SPEYP+LLEEQV+ CKRVLNIYRYMVM+ +M++RTWEQLL +LLQIT L + ++PP+R+E+TLGG+LA AIFQTLIVTWIKANL V++SPELWD+FL VLSSLT WEELIREWAKT+ETLTRVLAR VY LDLNDLPLDRL+E+ AKKRRG  Q+GG Q    +SS     S I      +S+ H P         S   +P+      R  GSP  RR             R  SE+   Y      R+ +   R     +   +    ++Q   +L+      + H + +    R+ RSV+S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + +  +H+Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PP  I APWC KAL LP +YQ+GKL A RLLC +T  P D  L + HL QFYKVLH+GL G+   V+NTL++++GPRFFSLMLPG++  + DF+ AAN  +S+S+L+G PRTEA S++GALL  P+   +  +L PN+ E +++  +D KD I+ +LLK GK+EPAG ARCI+LSSLGI++Y EL H  FHPK KEA+ VLL ALRFNNK V Q+ASD+LLLL DH    ++ YPE+P +I+EVLA TL  L P+     +E +K LL SL+FCLGEWCM+IP +++L     +G  LL  VF+ L Q                        +  G   +   +  P  +V DFDPNI +D+ KEG     ++P    +S +  QE+ S+         SN  +++AA+ V+ HLVNHL HFPM  GAA LSS+V E DD+PGL TDEL  +VF  PNIQLF+L+N+ ++SLVELP +E         ++T+  QVR+ILRD+SGK SW          DS   + LTK   + +G+     SK       L + +L SS  G +  S      +R R   +LPT +N AEDMDNLDDLLQY+ +TSPEC E +  P N  A P   L   E E D I+ V++QR  EQ+++++ + ++T  A      P  P Q        S FQ CR LFS LGL+ WE+R  +HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+R+QVSRK EVPYFGPLFNE IV  +VLPGL+RA+AI ASRAKRSML FYQ++YEER+K+LE ++ NHKD TTFEEF  QV+SP   T+ F
Sbjct:    1 MFSKKLH-----ADVKKSTLKIQDLKKDSATRLKHLKIILENVDAEEAKSVFESNFSHIYYILYDTFVTAEANLRQRELSFHLVHKAHREELECVLQVLEKVLVYLPELLSRRWQCHSLTRIMTKLLHPGNSWKL-RREAIRYFLLWYQSLADNSPEALHAIFATLVPGFPSPFAGQGLSALNRSSTSVFHDISRGPVNAVEIQPLLPPQSGEK---QPD-DPMRYFLDALLEFIVSQVTKIEWRDRLC-KQHKCFVFLFERFKQYYLPHIFPQFSFATSLYKPVLDLPVLRKGSPGLSDTPEGRRRQDPFVVCRVVVIKWVATFTHITKKGEVPFMHTQSNSSVTPSEDTDHSPGTELRRVSVSQAGSNHDAASPASDSNHSSVFSQSEGGNHSLSEDP--NAAAMHLVCEVLYSSRENVNFVHEVYRQAFLLNFTHSPAIRKAIAVYKDWIQMNVPELPSFMLEPLESHKEDIYRSGPPSYEYSSMESSEAAESYRVTRLRNDSYLGAIHKENLLVRAGLQNVLQVFITHAANVFMLEVSPEYPLLLEEQVDTCKRVLNIYRYMVMHTRMESRTWEQLLMVLLQITSLILSKTPPKRKEETLGGKLAPAIFQTLIVTWIKANLNVIISPELWDRFLVVLSSLTQWEELIREWAKTLETLTRVLARHVYNLDLNDLPLDRLTEQKAKKRRGGKQQGGDQPQASFSSTGRRGSEISSQGKTLSQSHGP--SQPEDGLSSYTSPEIGLGLLRLHGSPGKRRDSSGDSSQRSHLPRSVSESNLMYGQCTPSRKMYHTSRPQNNTVVIPVLPISVEQEVARLMASPQSSSIHCQPRNRLCRKSRSVDSLRGARSGTPNQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLSGGDGTSLD---LPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLTNPALHSQVFEYLVELCDTLAKIRLNQGVSDDNQVTPPPPDLIPPLNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISLPRDHLSQFYKVLHQGLVGTDQAVINTLVQFSGPRFFSLMLPGHSLLLYDFIHAANTIVSSSDLRGTPRTEAVSILGALLCLPNTFAETLVLQPNAGEFTMMPCSDAKDHILSILLKCGKREPAGQARCIALSSLGIYLYQELTHEIFHPKNKEAMNVLLLALRFNNKTVGQVASDILLLLCDHADRLVEHYPEIPSRIIEVLALTLSHLMPQNNVTTSERDKHLLTSLMFCLGEWCMKIPANYLLA--SNNGSCLLLTVFKVLNQL------------------QGGGGSGSGVSGSTGGLFQPD-LVQDFDPNILLDDLKEG-----STPPVGRRSSAMIQESGSLSHHHH----SNQSIQMAARMVMMHLVNHLGHFPMGIGAARLSSMVVEHDDVPGLATDELSAEVFSAPNIQLFVLSNSLMVSLVELPALEVPGGGITAGLRTAPSQVRIILRDLSGKASWDASILYCSPEDSICSHRLTKEEETEDGVGICGSSKPISVSSRLEEAMLTSSFVGGELHSCPPQHTLRHRPPHMLPTCDNGAEDMDNLDDLLQYLGYTSPECLESLDTPRNIPATPPPPL-TREAEEDTITMVLNQRNAEQEYLQRRSADITMLAGCMATFPHKPSQ--------SPFQHCRLLFSQLGLAGWERRSQLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKSSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRVQVSRKSEVPYFGPLFNESIVDDKVLPGLVRASAIGASRAKRSMLPFYQNYYEERAKSLETVVRNHKDATTFEEFTAQVYSPVQATSPF 1886          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1339045763|ref|XP_023711024.1| (probable Rho GTPase-activating protein CG5521 isoform X2 [Cryptotermes secundus])

HSP 1 Score: 1846.63 bits (4782), Expect = 0.000e+0
Identity = 1023/1946 (52.57%), Postives = 1305/1946 (67.06%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKG-----AHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTAL----AAMTPDTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKMDPMLSCRVVVIKWVAQYTHITRSGAM------GSSGTLAASNLGLNGT----MDVLQSGLN------LSMSEKDTSF-QVEHMSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKE---RDELDYAMTGKLSADMADPNDSGYRLRNDSYLGAIHKEHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRG--QRGGPQ-DLKYSS-----SSIKQPVTPVSEVHPPILDDSSGAGSLGGAPQTQ---FRRQGSPYPRRK------------RISSETE--YKSSPNERRQHQHVRFYPTNLRRSMSEGYIKQPPTQLIG---EYNFHLESK----RRHRSVESMYTYSR------SESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIP-HFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSW----------DSCAMNGLTK---SPNGIIEMDYSK-------LAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN--AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA-----HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1846
            MFSKK +      DVKKS  K+ D K+D+ TR KHL+I L+N D  E    FE+ +SHI+YI+YD F+TAE NLRQ+       HKA REELE VL + EK+L+YLPE L R+WQ HS+ R+M+KLLHPGNSWKL +REA+R F+LWYQ L D + E LHA+FATLVPGFPSP+ G GL+AL     ++  D   GPVN  EI PL+PPQSGE+    PD D  R FL+ LLE +VSQV  IEW++    +Q +C  FLF++FK YY+PHIFP+F++  SLY P L+LP +RK  P   DT +G ++ DP + CRVVVIKWVA +THIT+ G +       +S T +       GT    + V Q+G N       S S   + F Q E  + S  ED   N+   ++V  VLY++RENV+FVHEVYRQAFLL+F+HSPAI+K I VYKDWIQMNV ELP F+LEPL   KE   R          + +  A  +    RLRNDSYLGAIHKE+L VRAGLQNVLQ+FIT+AANVF+LE+SPEYP+LLEEQV+ CKRVLNIYRYMVM+ +M++RTWEQLL +LLQIT L + ++PP+R+E+TLGG+LA AIFQTLIVTWIKANL V++SPELWD+FL VLSSLT WEELIREWAKT+ETLTRVLAR VY LDLNDLPLDRL+E+ AKKRRG  Q+GG Q    +SS     S I      +S+ H P         S   +P+      R  GSP  RR             R  SE+   Y      R+ +   R     +   +    ++Q   +L+      + H + +    R+ RSV+S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + +  +H+Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PP  I APWC KAL LP +YQ+GKL A RLLC +T  P D  L + HL QFYKVLH+GL G+   V+NTL++++GPRFFSLMLPG++  + DF+ AAN  +S+S+L+G PRTEA S++GALL  P+   +  +L PN+ E +++  +D KD I+ +LLK GK+EPAG ARCI+LSSLGI++Y EL H  FHPK KEA+ VLL ALRFNNK V Q+ASD+LLLL DH    ++ YPE+P +I+EVLA TL  L P+     +E +K LL SL+FCLGEWCM+IP +++L     +G  LL  VF+ L Q                        +  G   +   +  P  +V DFDPNI +D+ KEG     ++P    +S +  QE+ S+         SN  +++AA+ V+ HLVNHL HFPM  GAA LSS+V E DD+PGL TDEL  +VF  PNIQLF+L+N+ ++SLVELP +E         ++T+  QVR+ILRD+SGK SW          DS   + LTK   + +G+     SK       L + +L SS  G +  S      +R R   +LPT +N AEDMDNLDDLLQY+ +TSPEC E +  P N  A P   L   E E D I+ V++QR  EQ+++++ + ++T  A      P  P Q        S FQ CR LFS LGL+ WE+R  +HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+R+QVSRK EVPYFGPLFNE IV  +VLPGL+RA+AI ASRAKRSML FYQ++YEER+K+LE ++ NHKD TTFEEF  QV+SP   T+ F
Sbjct:    1 MFSKKLH-----ADVKKSTLKIQDLKKDSATRLKHLKIILENVDAEEAKSVFESNFSHIYYILYDTFVTAEANLRQRELSFHLVHKAHREELECVLQVLEKVLVYLPELLSRRWQCHSLTRIMTKLLHPGNSWKL-RREAIRYFLLWYQSLADNSPEALHAIFATLVPGFPSPFAGQGLSALNRSSTSVFHDISRGPVNAVEIQPLLPPQSGEK---QPD-DPMRYFLDALLEFIVSQVTKIEWRDRLC-KQHKCFVFLFERFKQYYLPHIFPQFSFATSLYKPVLDLPVLRKGSPGLSDTPEGRRRQDPFVVCRVVVIKWVATFTHITKKGEVPFMHTQSNSVTPSEDTDHSPGTELRRVSVSQAGSNHDAASPASDSNHSSVFSQSEGGNHSLSEDP--NAAAMHLVCEVLYSSRENVNFVHEVYRQAFLLNFTHSPAIRKAIAVYKDWIQMNVPELPSFMLEPLESHKEDIYRSGPPSYEYSSMESSEAAESYRVTRLRNDSYLGAIHKENLLVRAGLQNVLQVFITHAANVFMLEVSPEYPLLLEEQVDTCKRVLNIYRYMVMHTRMESRTWEQLLMVLLQITSLILSKTPPKRKEETLGGKLAPAIFQTLIVTWIKANLNVIISPELWDRFLVVLSSLTQWEELIREWAKTLETLTRVLARHVYNLDLNDLPLDRLTEQKAKKRRGGKQQGGDQPQASFSSTGRRGSEISSQGKTLSQSHGP--SQPEDGLSSYTSPEIGLGLLRLHGSPGKRRDSSGDSSQRSHLPRSVSESNLMYGQCTPSRKMYHTSRPQNNTVVIPVLPISVEQEVARLMASPQSSSIHCQPRNRLCRKSRSVDSLRGARSGTPNQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLSGGDGTSLD---LPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLTNPALHSQVFEYLVELCDTLAKIRLNQGVSDDNQVTPPPPDLIPPLNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISLPRDHLSQFYKVLHQGLVGTDQAVINTLVQFSGPRFFSLMLPGHSLLLYDFIHAANTIVSSSDLRGTPRTEAVSILGALLCLPNTFAETLVLQPNAGEFTMMPCSDAKDHILSILLKCGKREPAGQARCIALSSLGIYLYQELTHEIFHPKNKEAMNVLLLALRFNNKTVGQVASDILLLLCDHADRLVEHYPEIPSRIIEVLALTLSHLMPQNNVTTSERDKHLLTSLMFCLGEWCMKIPANYLLA--SNNGSCLLLTVFKVLNQL------------------QGGGGSGSGVSGSTGGLFQPD-LVQDFDPNILLDDLKEG-----STPPVGRRSSAMIQESGSLSHHHH----SNQSIQMAARMVMMHLVNHLGHFPMGIGAARLSSMVVEHDDVPGLATDELSAEVFSAPNIQLFVLSNSLMVSLVELPALEVPGGGITAGLRTAPSQVRIILRDLSGKASWDASILYCSPEDSICSHRLTKEEETEDGVGICGSSKPISVSSRLEEAMLTSSFVGGELHSCPPQHTLRHRPPHMLPTCDNGAEDMDNLDDLLQYLGYTSPECLESLDTPRNIPATPPPPL-TREAEEDTITMVLNQRNAEQEYLQRRSADITMLAGCMATFPHKPSQ--------SPFQHCRLLFSQLGLAGWERRSQLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKSSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRVQVSRKSEVPYFGPLFNESIVDDKVLPGLVRASAIGASRAKRSMLPFYQNYYEERAKSLETVVRNHKDATTFEEFTAQVYSPVQATSPF 1885          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1339045767|ref|XP_023711026.1| (probable Rho GTPase-activating protein CG5521 isoform X4 [Cryptotermes secundus])

HSP 1 Score: 1845.48 bits (4779), Expect = 0.000e+0
Identity = 1022/1947 (52.49%), Postives = 1305/1947 (67.03%), Query Frame = 0

Query:    1 MFSKKPNLSKKSEDVKKSAGKVVDPKRDTPTRAKHLRIFLDNADLHEIIPFFEAYYSHIFYIVYDAFITAEINLRQKG-----AHKAQREELEAVLYLFEKILLYLPEHLGRKWQYHSVGRLMSKLLHPGNSWKLLKREAMRLFILWYQVLGDVAGEPLHAMFATLVPGFPSPYQGLGLTAL----AAMTPDTQDGPVNPAEICPLIPPQSGERWRTSPDTDLTRIFLEVLLELVVSQVPLIEWKENKSERQKRCLDFLFDKFKAYYMPHIFPEFNWNMSLYNPSLNLPEVRKFRPAFIDTGDGTKKMDPMLSCRVVVIKWVAQYTHITRSGAMGSSGTLAASNLGLNGTMD-----------VLQSGLN------LSMSEKDTSF-QVEHMSLSPFEDNVRNSLEGNMVRSVLYANRENVDFVHEVYRQAFLLSFSHSPAIKKVITVYKDWIQMNVVELPPFLLEPLHVDKE---RDELDYAMTGKLSADMADPNDSGYRLRNDSYLGAIHKEHLAVRAGLQNVLQIFITNAANVFLLEISPEYPILLEEQVEMCKRVLNIYRYMVMNVKMDARTWEQLLFILLQITQLTMPESPPRRREDTLGGRLAQAIFQTLIVTWIKANLYVVVSPELWDQFLEVLSSLTLWEELIREWAKTMETLTRVLARQVYQLDLNDLPLDRLSERAAKKRRG--QRGGPQ-DLKYSS-----SSIKQPVTPVSEVHPPILDDSSGAGSLGGAPQTQ---FRRQGSPYPRRK------------RISSETE--YKSSPNERRQHQHVRFYPTNLRRSMSEGYIKQPPTQLIG---EYNFHLESK----RRHRSVESMYTYSR------SESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIP-HFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSW----------DSCAMNGLTK---SPNGIIEMDYSK-------LAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN--AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA-----HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1846
            MFSKK +      DVKKS  K+ D K+D+ TR KHL+I L+N D  E    FE+ +SHI+YI+YD F+TAE NLRQ+       HKA REELE VL + EK+L+YLPE L R+WQ HS+ R+M+KLLHPGNSWKL +REA+R F+LWYQ L D + E LHA+FATLVPGFPSP+ G GL+AL     ++  D   GPVN  EI PL+PPQSGE+    PD D  R FL+ LLE +VSQV  IEW++    +Q +C  FLF++FK YY+PHIFP+F++  SLY P L+LP +RK  P   DT +G ++ DP + CRVVVIKWVA +THIT+ G +    T + S++  +   D           V Q+G N       S S   + F Q E  + S  ED   N+   ++V  VLY++RENV+FVHEVYRQAFLL+F+HSPAI+K I VYKDWIQMNV ELP F+LEPL   KE   R          + +  A  +    RLRNDSYLGAIHKE+L VRAGLQNVLQ+FIT+AANVF+LE+SPEYP+LLEEQV+ CKRVLNIYRYMVM+ +M++RTWEQLL +LLQIT L + ++PP+R+E+TLGG+LA AIFQTLIVTWIKANL V++SPELWD+FL VLSSLT WEELIREWAKT+ETLTRVLAR VY LDLNDLPLDRL+E+ AKKRRG  Q+GG Q    +SS     S I      +S+ H P         S   +P+      R  GSP  RR             R  SE+   Y      R+ +   R     +   +    ++Q   +L+      + H + +    R+ RSV+S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + +  +H+Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PP  I APWC KAL LP +YQ+GKL A RLLC +T  P D  L + HL QFYKVLH+GL G+   V+NTL++++GPRFFSLMLPG++  + DF+ AAN  +S+S+L+G PRTEA S++GALL  P+   +  +L PN+ E +++  +D KD I+ +LLK GK+EPAG ARCI+LSSLGI++Y EL H  FHPK KEA+ VLL ALRFNNK V Q+ASD+LLLL DH    ++ YPE+P +I+EVLA TL  L P+     +E +K LL SL+FCLGEWCM+IP +++L     +G  LL  VF+ L Q                        +  G   +   +  P  +V DFDPNI +D+ KEG     ++P    +S +  QE+ S+         SN  +++AA+ V+ HLVNHL HFPM  GAA LSS+V E DD+PGL TDEL  +VF  PNIQLF+L+N+ ++SLVELP +E         ++T+  QVR+ILRD+SGK SW          DS   + LTK   + +G+     SK       L + +L SS  G +  S      +R R   +LPT +N AEDMDNLDDLLQY+ +TSPEC E +  P N  A P   L   E E D I+ V++QR  EQ+++++ + ++T  A      P  P Q        S FQ CR LFS LGL+ WE+R  +HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+R+QVSRK EVPYFGPLFNE IV  +VLPGL+RA+AI ASRAKRSML FYQ++YEER+K+LE ++ NHKD TTFEEF  QV+SP   T+ F
Sbjct:    1 MFSKKLH-----ADVKKSTLKIQDLKKDSATRLKHLKIILENVDAEEAKSVFESNFSHIYYILYDTFVTAEANLRQRELSFHLVHKAHREELECVLQVLEKVLVYLPELLSRRWQCHSLTRIMTKLLHPGNSWKL-RREAIRYFLLWYQSLADNSPEALHAIFATLVPGFPSPFAGQGLSALNRSSTSVFHDISRGPVNAVEIQPLLPPQSGEK---QPD-DPMRYFLDALLEFIVSQVTKIEWRDRLC-KQHKCFVFLFERFKQYYLPHIFPQFSFATSLYKPVLDLPVLRKGSPGLSDTPEGRRRQDPFVVCRVVVIKWVATFTHITKKGEVPFMHTQSNSSVTPSEDTDHSPGTELRRVSVSQAGSNHDAASPASDSNHSSVFSQSEGGNHSLSEDP--NAAAMHLVCEVLYSSRENVNFVHEVYRQAFLLNFTHSPAIRKAIAVYKDWIQMNVPELPSFMLEPLESHKEDIYRSGPPSYEYSSMESSEAAESYRVTRLRNDSYLGAIHKENLLVRAGLQNVLQVFITHAANVFMLEVSPEYPLLLEEQVDTCKRVLNIYRYMVMHTRMESRTWEQLLMVLLQITSLILSKTPPKRKEETLGGKLAPAIFQTLIVTWIKANLNVIISPELWDRFLVVLSSLTQWEELIREWAKTLETLTRVLARHVYNLDLNDLPLDRLTEQKAKKRRGGKQQGGDQPQASFSSTGRRGSEISSQGKTLSQSHGP--SQPEDGLSSYTSPEIGLGLLRLHGSPGKRRDSSGDSSQRSHLPRSVSESNLMYGQCTPSRKMYHTSRPQNNTVVIPVLPISVEQEVARLMASPQSSSIHCQPRNRLCRKSRSVDSLRGARSGTPNQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLSGGDGTSLD---LPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLTNPALHSQVFEYLVELCDTLAKIRLNQGVSDDNQVTPPPPDLIPPLNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISLPRDHLSQFYKVLHQGLVGTDQAVINTLVQFSGPRFFSLMLPGHSLLLYDFIHAANTIVSSSDLRGTPRTEAVSILGALLCLPNTFAETLVLQPNAGEFTMMPCSDAKDHILSILLKCGKREPAGQARCIALSSLGIYLYQELTHEIFHPKNKEAMNVLLLALRFNNKTVGQVASDILLLLCDHADRLVEHYPEIPSRIIEVLALTLSHLMPQNNVTTSERDKHLLTSLMFCLGEWCMKIPANYLLA--SNNGSCLLLTVFKVLNQL------------------QGGGGSGSGVSGSTGGLFQPD-LVQDFDPNILLDDLKEG-----STPPVGRRSSAMIQESGSLSHHHH----SNQSIQMAARMVMMHLVNHLGHFPMGIGAARLSSMVVEHDDVPGLATDELSAEVFSAPNIQLFVLSNSLMVSLVELPALEVPGGGITAGLRTAPSQVRIILRDLSGKASWDASILYCSPEDSICSHRLTKEEETEDGVGICGSSKPISVSSRLEEAMLTSSFVGGELHSCPPQHTLRHRPPHMLPTCDNGAEDMDNLDDLLQYLGYTSPECLESLDTPRNIPATPPPPL-TREAEEDTITMVLNQRNAEQEYLQRRSADITMLAGCMATFPHKPSQ--------SPFQHCRLLFSQLGLAGWERRSQLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKSSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRVQVSRKSEVPYFGPLFNESIVDDKVLPGLVRASAIGASRAKRSMLPFYQNYYEERAKSLETVVRNHKDATTFEEFTAQVYSPVQATSPF 1886