Major vault protein is part of an extracellular cement material in the Atlantic salmon louse (Lepeophtheirus salmonis)

Overview
TitleMajor vault protein is part of an extracellular cement material in the Atlantic salmon louse (Lepeophtheirus salmonis)
AuthorsSlinning MS, Nthiga TM, Eichner C, Khadija S, Rome LH, Nilsen F, Dondrup M
TypeJournal Article
Journal NameScientific reports
Volume14
Issue1
Year2024
Page(s)15240
CitationSlinning MS, Nthiga TM, Eichner C, Khadija S, Rome LH, Nilsen F, Dondrup M. Major vault protein is part of an extracellular cement material in the Atlantic salmon louse (Lepeophtheirus salmonis). Scientific reports. 2024 Jul 02; 14(1):15240.

Abstract

Major vault protein (MVP) is the main component of the vault complex, which is a highly conserved ribonucleoprotein complex found in most eukaryotic organisms. MVP or vaults have previously been found to be overexpressed in multidrug-resistant cancer cells and implicated in various cellular processes such as cell signaling and innate immunity. The precise function of MVP is, however, poorly understood and its expression and probable function in lower eukaryotes are not well characterized. In this study, we report that the Atlantic salmon louse expresses three full-length MVP paralogues (LsMVP1-3). Furthermore, we extended our search and identified MVP orthologues in several other ecdysozoan species. LsMVPs were shown to be expressed in various tissues at both transcript and protein levels. In addition, evidence for LsMVP to assemble into vaults was demonstrated by performing differential centrifugation. LsMVP was found to be highly expressed in cement, an extracellular material produced by a pair of cement glands in the adult female salmon louse. Cement is important for the formation of egg strings that serve as protective coats for developing embryos. Our results imply a possible novel function of LsMVP as a secretory cement protein. LsMVP may play a role in structural or reproductive functions, although this has to be further investigated.

Properties
Additional details for this publication include:
Property NameValue
Journal CountryEngland
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
Copyright© 2024. The Author(s).
DOI10.1038/s41598-024-65683-0
Elocation10.1038/s41598-024-65683-0
PII15240
Journal AbbreviationSci Rep
Publication Date2024 Jul 02
eISSN2045-2322
ISSN2045-2322
Publication ModelElectronic
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PMID: PMID:38956386