Unusually high numbers of ribosomal RNA genes in copepods (Arthropoda: Crustacea) and their relationship to genome size

Overview
TitleUnusually high numbers of ribosomal RNA genes in copepods (Arthropoda: Crustacea) and their relationship to genome size
AuthorsWyngaard GA, McLaren IA, White MM, Sévigny JM
TypeJournal Article
Journal NameGenome / National Research Council Canada = Génome / Conseil national de recherches Canada
Volume38
Issue1
Year1995
Page(s)97-104
CitationWyngaard GA, McLaren IA, White MM, Sévigny JM. Unusually high numbers of ribosomal RNA genes in copepods (Arthropoda: Crustacea) and their relationship to genome size. Genome / National Research Council Canada = Génome / Conseil national de recherches Canada. 1995 Feb; 38(1):97-104.

Abstract

We report on copy numbers of 18S ribosomal RNA genes in three species of copepods (Crustacea: Copepoda), two of which possess an unusual arrangement in which 5S genes are included within the 18S-5.8S-28S repeat unit. Slot blots of genomic and standard DNA were hybridized with an 18S rRNA gene probe constructed from one of the marine species and hybridization was quantified using chemiluminescence. Diploid 18S rRNA gene copy numbers are estimated as ca. 15 300 and 33 500 in the marine species Calanus finmarchicus (13.0 pg DNA in 2C adult nuclei) and C. glacialis (24.2 pg DNA), respectively, and ca. 840 and 730 in two freshwater populations of Mesocyclops edax (both ca. 3 pg DNA) from Virginia and Nova Scotia, respectively. The roughly proportional relationship between 2C somatic nuclear DNA contents and rRNA gene copy number in the sibling species C. finmarchicus and C. glacialis may reflect polytenic replication of entire genomes during abrupt speciation events. Copy numbers may also reflect differential losses during embryonic chromatin diminution.

Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint
ISSN0831-2796
pISSN0831-2796
Publication Date1995 Feb
Journal AbbreviationGenome
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryCanada
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PMID: PMID:18470156