Molecular characterization and expression of vitellogenin (Vg) genes from the cyclopoid copepod, Paracyclopina nana exposed to heavy metals

Overview
TitleMolecular characterization and expression of vitellogenin (Vg) genes from the cyclopoid copepod, Paracyclopina nana exposed to heavy metals
AuthorsHwang DS, Lee KW, Han J, Park HG, Lee J, Lee YM, Lee JS
TypeJournal Article
Journal NameComparative biochemistry and physiology. Toxicology & pharmacology : CBP
Volume151
Issue3
Year2010
Page(s)360-8
CitationHwang DS, Lee KW, Han J, Park HG, Lee J, Lee YM, Lee JS. Molecular characterization and expression of vitellogenin (Vg) genes from the cyclopoid copepod, Paracyclopina nana exposed to heavy metals. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 2010 Apr; 151(3):360-8.

Abstract

Induction of vitellogenin (Vg) has been used as a biomarker of exposure to heavy metals and endocrine-disruption chemicals (EDCs) in aquatic organisms. Here, we identified the full-length Vg1 and Vg2 sequence from the brackish water copepod, Paracyclopina nana. Vg1 gene contained 5718bp of the open reading frame (ORF) that encoded the putative protein of 1905 amino acids residues, while Vg2 gene consisted of 5442bp of ORF, encoding the putative protein of 1813 amino acids residues. P. nana Vgs showed highly conserved domains in the N-terminal region. The phylogenetic analysis revealed that P. nana Vgs are distinct from other arthropods, such as insects and decapods, as it formed a clade with other copepods, Tigriopus japonicus and salmon louse (Lepeophtheirus salmonis). The expression of Vg transcripts was detectable after the copepodid stages 4-5. Female copepods expressed over 83 times and 223 times more Vg1 and Vg2 transcripts, respectively, than males. When copepods were exposed to heavy metals (0.1mg/L Cd, 0.4mg/L Cu, and 2mg/L AsIII) for 24, 48, 72, and 96h, P. nana Vg transcripts were highly induced in a time-dependent manner. Interestingly, Vg2 gene was more susceptible than Vg1 to trace heavy metal exposure. This finding indicates that P. nana Vgs provide a potential indicator for assessing the toxic effect of heavy metals. In addition, we suggest P. nana as a potential model species for risk-assessment to environmental pollutants in brackish water.

Author Details
Additional information about authors:
Details
1Dae-Sik Hwang
2Kyun-Woo Lee
3Jeonghoon Han
4Heum Gi Park
5Jehee Lee
6Young-Mi Lee
7Jae-Seong Lee
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1532-0456
pISSN1532-0456
Publication Date2010 Apr
Journal AbbreviationComp. Biochem. Physiol. C Toxicol. Pharmacol.
DOI10.1016/j.cbpc.2009.12.010
Elocation10.1016/j.cbpc.2009.12.010
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryUnited States
Publication TypeResearch Support, Non-U.S. Gov't
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PMID: PMID:20045491