BLAST of EMLSAG00000006819 vs. GO
Match: - (symbol:Nelf-E "Negative elongation factor E" species:7227 "Drosophila melanogaster" [GO:0003729 "mRNA binding" evidence=ISS;IDA] [GO:0007070 "negative regulation of transcription from RNA polymerase II promoter during mitosis" evidence=IDA] [GO:0017053 "transcriptional repressor complex" evidence=IDA] [GO:0000166 "nucleotide binding" evidence=IEA] [GO:0000122 "negative regulation of transcription from RNA polymerase II promoter" evidence=IMP] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IMP] [GO:0032021 "NELF complex" evidence=IPI] [GO:0034244 "negative regulation of transcription elongation from RNA polymerase II promoter" evidence=IDA] [GO:0005634 "nucleus" evidence=IDA] [GO:0022008 "neurogenesis" evidence=IMP] InterPro:IPR000504 InterPro:IPR012677 Pfam:PF00076 PROSITE:PS50102 SMART:SM00360 GO:GO:0017053 GO:GO:0005694 EMBL:AE014296 GO:GO:0022008 GO:GO:0000166 Gene3D:3.30.70.330 GO:GO:0003729 GO:GO:0045944 GO:GO:0006368 GO:GO:0034244 GO:GO:0007070 GO:GO:0032021 eggNOG:NOG86140 GeneTree:ENSGT00630000089917 KO:K15182 OMA:YNDDVYK OrthoDB:EOG7SV0W7 EMBL:Y10015 EMBL:Y10016 EMBL:AY071061 RefSeq:NP_648241.1 UniGene:Dm.20046 ProteinModelPortal:P92204 SMR:P92204 BioGrid:64390 IntAct:P92204 MINT:MINT-779659 STRING:7227.FBpp0076374 PaxDb:P92204 PRIDE:P92204 EnsemblMetazoa:FBtr0076650 GeneID:38982 KEGG:dme:Dmel_CG5994 CTD:38982 FlyBase:FBgn0017430 InParanoid:P92204 PhylomeDB:P92204 SignaLink:P92204 GenomeRNAi:38982 NextBio:811294 PRO:PR:P92204 Bgee:P92204 Uniprot:P92204)

HSP 1 Score: 218.009 bits (554), Expect = 1.269e-67
Identity = 137/288 (47.57%), Postives = 190/288 (65.97%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPS-ESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWER--KLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER-----------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEE MLQAKY KLKKKKK L A KAPKP  ES  T K+  +A+D++EVA+KLI++G + AIQK  ++ + S  FKR +G ER  + +    ++SYQPFS+T          ++ +  EP       + ++LY+ F + RDREER           + +K ++ NT++V G  V+E+ L+ TF+  G IVN+SMEI+K+ GF++F K +SA+RAI++ H  +V G+ L+V  ARRQ  I+PINDASSSA WSSIAAS SQK SH D R +++YDED  
Sbjct:    1 MVYIHFPNNLTEEEQMLQAKYQKLKKKKKALQAHKAPKPEPESSLTLKRPTDARDAREVARKLIKSGAIPAIQKQTKQDQTS--FKRPKGQERAKRSTSETTVASYQPFSSTQNDVAQETIISEIIKEEP-------RRQNLYQHFATERDREERGMPEKVPMDTAQPEKPRAGNTIFVSGNKVTEDFLKKTFNDYGTIVNVSMEIEKSRGFVSFAKPESADRAIAEIHGKNVNGINLQVQLARRQPQIEPINDASSSAVWSSIAASKSQKGSHKDHREMVQYDEDFL 279          

BLAST of EMLSAG00000006819 vs. GO
Match: - (symbol:Nelf-E "Negative elongation factor E" species:7244 "Drosophila virilis" [GO:0003723 "RNA binding" evidence=ISS] [GO:0007070 "negative regulation of transcription from RNA polymerase II promoter during mitosis" evidence=ISS] [GO:0017053 "transcriptional repressor complex" evidence=ISS] InterPro:IPR000504 InterPro:IPR012677 Pfam:PF00076 PROSITE:PS50102 SMART:SM00360 GO:GO:0017053 GO:GO:0005694 GO:GO:0000166 Gene3D:3.30.70.330 GO:GO:0006351 GO:GO:0003723 GO:GO:0007070 eggNOG:NOG86140 OrthoDB:EOG7SV0W7 EMBL:AJ306692 ProteinModelPortal:Q95ZE9 FlyBase:FBgn0044888 InParanoid:Q95ZE9 Uniprot:Q95ZE9)

HSP 1 Score: 197.978 bits (502), Expect = 6.675e-60
Identity = 133/288 (46.18%), Postives = 185/288 (64.24%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPS-ESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWER--KLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER-----------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEE MLQAKY KLKKKKK L A KAPK   ES  T K+  +A+D++EVA+KLI++G + AIQK  +  +    FKR +G ER  + +    +++YQPFS+T          ++ +  EP       + ++LY+ F + R REER           + +K ++ NT++V G  V+EE L+ TF+  G IVN+SMEI+K+ GF++F K +SA+RAI++ H  SV G+ L+V  ARRQ  I PINDASSSA WSSIAAS SQK SH D R +++Y++D  
Sbjct:    1 MVYIHFPNNLTEEEHMLQAKYQKLKKKKKALQAHKAPKQEPESALTLKRPTDARDAREVARKLIKSGAIPAIQKQ-QTKQDQTSFKRPKGQERAKRSTSETTVAAYQPFSSTQNDVAQETIISEIIKEEP-------RRQNLYQHFATERVREERGLTEKVTLDTTQPEKPRAGNTIFVSGNKVTEEFLKKTFNDYGTIVNVSMEIEKSRGFVSFAKPESADRAIAEMHGKSVTGIVLQVQLARRQPQIVPINDASSSAVWSSIAASKSQKGSHKDLRQMVQYNDDFL 280          

BLAST of EMLSAG00000006819 vs. GO
Match: - (symbol:R4GJG8 "Uncharacterized protein" species:9031 "Gallus gallus" [GO:0000166 "nucleotide binding" evidence=IEA] [GO:0003676 "nucleic acid binding" evidence=IEA] [GO:0005739 "mitochondrion" evidence=IEA] [GO:0032021 "NELF complex" evidence=IEA] [GO:0034244 "negative regulation of transcription elongation from RNA polymerase II promoter" evidence=IEA] InterPro:IPR000504 InterPro:IPR012677 Pfam:PF00076 PROSITE:PS50102 SMART:SM00360 GO:GO:0000166 Gene3D:3.30.70.330 GO:GO:0003676 GeneTree:ENSGT00630000089917 EMBL:AADN03008519 Ensembl:ENSGALT00000043201 Uniprot:R4GJG8)

HSP 1 Score: 112.464 bits (280), Expect = 3.347e-27
Identity = 50/117 (42.74%), Postives = 81/117 (69.23%), Query Frame = 0

Query:  158 KSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            +  NT+YVHG  +S ELLR+ FS  G I+++S++  +NC F+T++K +SA++A+++ +   V+ VQL+VS AR+Q ++D    A+  + W S+A  +S K SH DKR  + Y+EDLF
Sbjct:  241 RKGNTLYVHGAELSPELLRSAFSPFGAIIDLSLDSPRNCAFVTYEKMESADQAMAELNGAVVQDVQLRVSIARKQPLLD---AATGKSLWGSLAVKNSAKGSHRDKRAQVVYNEDLF 354          

HSP 2 Score: 46.2098 bits (108), Expect = 1.709e-4
Identity = 61/160 (38.12%), Postives = 81/160 (50.62%), Query Frame = 0

Query:    2 VYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPK----PSESEKTYKKVM------EAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDPR--VLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDR 149
                 P  LTEEE  LQ ++AKL+KKKK L ALK  +    PS S+   K+ M      +   + E AK L+++G + AI+       K+ GFKRS+  E KL DP      ++QPF  +          ADD  PE   P    + K LYESFVSA +R
Sbjct:    7 AMLTLPPGLTEEEEALQKRFAKLRKKKKALLALKKQQSNTGPS-SQGGIKRSMSDQPPVDTATATEQAKLLVKSGAISAIKAET----KNSGFKRSRTLEGKLKDPEKGPAPTFQPFQRS--------VSADDDTPESRRP----QRKSLYESFVSASER 149          

BLAST of EMLSAG00000006819 vs. GO
Match: - (symbol:NELFE "Uncharacterized protein" species:9823 "Sus scrofa" [GO:0000166 "nucleotide binding" evidence=IEA] [GO:0003676 "nucleic acid binding" evidence=IEA] InterPro:IPR000504 InterPro:IPR012677 Pfam:PF00076 PROSITE:PS50102 SMART:SM00360 GO:GO:0000166 Gene3D:3.30.70.330 GO:GO:0003676 GeneTree:ENSGT00630000089917 EMBL:CT956038 Ensembl:ENSSSCT00000032936 ArrayExpress:K7GQ47 Uniprot:K7GQ47)

HSP 1 Score: 105.916 bits (263), Expect = 8.306e-25
Identity = 51/126 (40.48%), Postives = 81/126 (64.29%), Query Frame = 0

Query:  149 REERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            R  RKG      NT+YV+G  ++  LLR  FS  G I+++SM+  +NC F+T++K +SA++A+++ + T V+ VQLKVS AR+Q ++D    A+  + W S+A  +S K  H DKR  I Y +D++
Sbjct:  249 RAPRKG------NTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVSIARKQPMLDA---ATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVY 365          

BLAST of EMLSAG00000006819 vs. GO
Match: - (symbol:NELFE "Negative elongation factor E" species:9913 "Bos taurus" [GO:0000166 "nucleotide binding" evidence=IEA] [GO:0003723 "RNA binding" evidence=IEA] [GO:0005739 "mitochondrion" evidence=IEA] [GO:0006351 "transcription, DNA-templated" evidence=IEA] [GO:0032021 "NELF complex" evidence=IEA] [GO:0034244 "negative regulation of transcription elongation from RNA polymerase II promoter" evidence=IEA] InterPro:IPR000504 InterPro:IPR012677 Pfam:PF00076 PROSITE:PS50102 SMART:SM00360 GO:GO:0005739 GO:GO:0000166 Gene3D:3.30.70.330 GO:GO:0006351 GO:GO:0003723 GO:GO:0034244 GO:GO:0032021 EMBL:BT026321 EMBL:BC120072 RefSeq:NP_001069672.1 UniGene:Bt.36000 ProteinModelPortal:Q0V898 SMR:Q0V898 STRING:9913.ENSBTAP00000009802 PaxDb:Q0V898 PRIDE:Q0V898 Ensembl:ENSBTAT00000009802 GeneID:540158 KEGG:bta:540158 CTD:7936 eggNOG:NOG86140 GeneTree:ENSGT00630000089917 HOGENOM:HOG000232089 HOVERGEN:HBG052598 InParanoid:Q0V898 KO:K15182 OMA:YNDDVYK OrthoDB:EOG7SV0W7 TreeFam:TF324087 NextBio:20878458 Uniprot:Q0V898)

HSP 1 Score: 105.531 bits (262), Expect = 9.084e-25
Identity = 51/126 (40.48%), Postives = 81/126 (64.29%), Query Frame = 0

Query:  149 REERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            R  RKG      NT+YV+G  ++  LLR  FS  G I+++SM+  +NC F+T++K +SA++A+++ + T V+ VQLKVS AR+Q ++D    A+  + W S+A  +S K  H DKR  I Y +D++
Sbjct:  250 RAPRKG------NTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVSIARKQPMLDA---ATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVY 366          

BLAST of EMLSAG00000006819 vs. GO
Match: - (symbol:NELFE "Uncharacterized protein" species:9823 "Sus scrofa" [GO:0000166 "nucleotide binding" evidence=IEA] [GO:0003676 "nucleic acid binding" evidence=IEA] [GO:0005739 "mitochondrion" evidence=IEA] [GO:0032021 "NELF complex" evidence=IEA] [GO:0034244 "negative regulation of transcription elongation from RNA polymerase II promoter" evidence=IEA] InterPro:IPR000504 InterPro:IPR012677 Pfam:PF00076 PROSITE:PS50102 SMART:SM00360 GO:GO:0005739 GO:GO:0000166 Gene3D:3.30.70.330 GO:GO:0003676 GO:GO:0034244 GO:GO:0032021 GeneTree:ENSGT00630000089917 OMA:YNDDVYK OrthoDB:EOG7SV0W7 TreeFam:TF324087 EMBL:CT956038 Ensembl:ENSSSCT00000001565 ArrayExpress:F1RQW5 Uniprot:F1RQW5)

HSP 1 Score: 105.531 bits (262), Expect = 9.880e-25
Identity = 51/126 (40.48%), Postives = 81/126 (64.29%), Query Frame = 0

Query:  149 REERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            R  RKG      NT+YV+G  ++  LLR  FS  G I+++SM+  +NC F+T++K +SA++A+++ + T V+ VQLKVS AR+Q ++D    A+  + W S+A  +S K  H DKR  I Y +D++
Sbjct:  254 RAPRKG------NTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVSIARKQPMLDA---ATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVY 370          

BLAST of EMLSAG00000006819 vs. GO
Match: - (symbol:Nelfe "negative elongation factor complex member E, Rdbp" species:10090 "Mus musculus" [GO:0000166 "nucleotide binding" evidence=IEA] [GO:0003676 "nucleic acid binding" evidence=IEA] [GO:0003723 "RNA binding" evidence=IEA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005634 "nucleus" evidence=IEA] [GO:0006351 "transcription, DNA-templated" evidence=IEA] [GO:0006355 "regulation of transcription, DNA-templated" evidence=IEA] [GO:0032021 "NELF complex" evidence=ISO] [GO:0034244 "negative regulation of transcription elongation from RNA polymerase II promoter" evidence=IMP] [GO:0044822 "poly(A) RNA binding" evidence=ISO] InterPro:IPR000504 InterPro:IPR012677 Pfam:PF00076 PROSITE:PS50102 SMART:SM00360 MGI:MGI:102744 GO:GO:0005739 GO:GO:0000166 Gene3D:3.30.70.330 GO:GO:0006351 GO:GO:0003723 GO:GO:0034244 EMBL:AF109906 UniGene:Mm.653 GO:GO:0032021 CTD:7936 eggNOG:NOG86140 HOGENOM:HOG000232089 HOVERGEN:HBG052598 KO:K15182 OMA:YNDDVYK OrthoDB:EOG7SV0W7 TreeFam:TF324087 EMBL:M21332 EMBL:AK012389 EMBL:BC034867 EMBL:U83841 EMBL:U83842 PIR:A40112 RefSeq:NP_001039328.1 RefSeq:NP_001039329.1 RefSeq:NP_613046.2 UniGene:Mm.279907 ProteinModelPortal:P19426 SMR:P19426 IntAct:P19426 PhosphoSite:P19426 PaxDb:P19426 PRIDE:P19426 Ensembl:ENSMUST00000097343 Ensembl:ENSMUST00000165953 Ensembl:ENSMUST00000173357 GeneID:27632 KEGG:mmu:27632 InParanoid:P19426 NextBio:305826 PRO:PR:P19426 ArrayExpress:P19426 Bgee:P19426 CleanEx:MM_RDBP Genevestigator:P19426 Uniprot:P19426)

HSP 1 Score: 104.76 bits (260), Expect = 1.716e-24
Identity = 51/125 (40.80%), Postives = 80/125 (64.00%), Query Frame = 0

Query:  149 REERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDL 273
            R  RKG      NT+YV+G  ++  LLR  FS  G I+++SM+  +NC F+T++K +SA++A+++ + T V+ VQLKV+ AR+Q ++D    A+  + W S+A  +S K  H DKR  I Y +DL
Sbjct:  260 RAPRKG------NTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLD---AATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDL 375          

BLAST of EMLSAG00000006819 vs. GO
Match: - (symbol:Nelfe "Protein Nelfe" species:10116 "Rattus norvegicus" [GO:0000166 "nucleotide binding" evidence=IEA] [GO:0003676 "nucleic acid binding" evidence=IEA] [GO:0005739 "mitochondrion" evidence=IEA] [GO:0032021 "NELF complex" evidence=IEA] [GO:0034244 "negative regulation of transcription elongation from RNA polymerase II promoter" evidence=IEA] InterPro:IPR000504 InterPro:IPR012677 Pfam:PF00076 PROSITE:PS50102 SMART:SM00360 RGD:1303325 GO:GO:0005739 GO:GO:0000166 Gene3D:3.30.70.330 GO:GO:0003676 EMBL:BX883045 GO:GO:0034244 EMBL:CH474121 GO:GO:0032021 CTD:7936 GeneTree:ENSGT00630000089917 HOGENOM:HOG000232089 HOVERGEN:HBG052598 KO:K15182 OMA:YNDDVYK OrthoDB:EOG7SV0W7 TreeFam:TF324087 EMBL:AABR06099865 EMBL:BC079427 RefSeq:NP_001159373.1 RefSeq:NP_997713.1 RefSeq:XP_006255984.1 UniGene:Rn.16164 STRING:10116.ENSRNOP00000000478 Ensembl:ENSRNOT00000000478 GeneID:294258 KEGG:rno:294258 UCSC:RGD:1303325 eggNOG:NOG247039 InParanoid:Q6MG75 NextBio:637845 PRO:PR:Q6MG75 Genevestigator:Q6MG75 Uniprot:Q6MG75)

HSP 1 Score: 104.375 bits (259), Expect = 2.094e-24
Identity = 51/125 (40.80%), Postives = 80/125 (64.00%), Query Frame = 0

Query:  149 REERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDL 273
            R  RKG      NT+YV+G  ++  LLR  FS  G I+++SM+  +NC F+T++K +SA++A+++ + T V+ VQLKV+ AR+Q ++D    A+  + W S+A  +S K  H DKR  I Y +DL
Sbjct:  254 RAPRKG------NTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLD---AATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDL 369          

BLAST of EMLSAG00000006819 vs. GO
Match: - (symbol:Nelfe "negative elongation factor complex member E" species:10116 "Rattus norvegicus" [GO:0000166 "nucleotide binding" evidence=IEA] [GO:0003676 "nucleic acid binding" evidence=IEA] [GO:0005739 "mitochondrion" evidence=IEA] [GO:0032021 "NELF complex" evidence=IEA;ISO] [GO:0034244 "negative regulation of transcription elongation from RNA polymerase II promoter" evidence=IEA;ISO] [GO:0044822 "poly(A) RNA binding" evidence=ISO] InterPro:IPR000504 InterPro:IPR012677 Pfam:PF00076 PROSITE:PS50102 SMART:SM00360 RGD:1303325 GO:GO:0005739 GO:GO:0000166 Gene3D:3.30.70.330 GO:GO:0003676 EMBL:BX883045 GO:GO:0034244 EMBL:CH474121 GO:GO:0032021 CTD:7936 GeneTree:ENSGT00630000089917 HOGENOM:HOG000232089 HOVERGEN:HBG052598 KO:K15182 OMA:YNDDVYK OrthoDB:EOG7SV0W7 TreeFam:TF324087 EMBL:AABR06099865 EMBL:BC079427 RefSeq:NP_001159373.1 RefSeq:NP_997713.1 RefSeq:XP_006255984.1 UniGene:Rn.16164 STRING:10116.ENSRNOP00000000478 Ensembl:ENSRNOT00000000478 GeneID:294258 KEGG:rno:294258 UCSC:RGD:1303325 eggNOG:NOG247039 InParanoid:Q6MG75 NextBio:637845 PRO:PR:Q6MG75 Genevestigator:Q6MG75 Uniprot:Q6MG75)

HSP 1 Score: 104.375 bits (259), Expect = 2.094e-24
Identity = 51/125 (40.80%), Postives = 80/125 (64.00%), Query Frame = 0

Query:  149 REERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDL 273
            R  RKG      NT+YV+G  ++  LLR  FS  G I+++SM+  +NC F+T++K +SA++A+++ + T V+ VQLKV+ AR+Q ++D    A+  + W S+A  +S K  H DKR  I Y +DL
Sbjct:  254 RAPRKG------NTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLD---AATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDL 369          

BLAST of EMLSAG00000006819 vs. GO
Match: - (symbol:NELFE "cDNA FLJ56180, highly similar to Negative elongation factor E" species:9606 "Homo sapiens" [GO:0000166 "nucleotide binding" evidence=IEA] [GO:0003746 "translation elongation factor activity" evidence=IEA] InterPro:IPR000504 InterPro:IPR012677 Pfam:PF00076 PROSITE:PS50102 SMART:SM00360 GO:GO:0005739 GO:GO:0005634 GO:GO:0000166 Gene3D:3.30.70.330 GO:GO:0003746 EMBL:AL645922 HOGENOM:HOG000232089 HOVERGEN:HBG052598 OMA:YNDDVYK UniGene:Hs.423935 HGNC:HGNC:13974 EMBL:AK302652 ProteinModelPortal:B4DYX9 SMR:B4DYX9 PRIDE:B4DYX9 Ensembl:ENST00000375425 NextBio:35476341 ArrayExpress:B4DYX9 Uniprot:B4DYX9)

HSP 1 Score: 103.99 bits (258), Expect = 3.163e-24
Identity = 46/117 (39.32%), Postives = 78/117 (66.67%), Query Frame = 0

Query:  158 KSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            +  NT+YV+G  ++  LLR  FS  G I+++SM+  +NC F+T++K +SA++A+++ + T V+ VQLKV+ AR+Q ++D    A+  + W S+A  +S K  H DKR  I Y +D++
Sbjct:  266 RKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDA---ATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVY 379          

BLAST of EMLSAG00000006819 vs. C. finmarchicus
Match: gi|592792813|gb|GAXK01161755.1| (TSA: Calanus finmarchicus comp34075_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 211.846 bits (538), Expect = 1.948e-65
Identity = 163/286 (56.99%), Postives = 215/286 (75.17%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMXXXXXXXXXXXXXXXXXXXXXXXSESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSK------GFKRSQGWERKLSD-PRVLSSYQPFSATHGPGGGFDTGADDLNXXXXXXXXXKKVKDLYESFVSARDREER-----KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDXXXXXXXXXXXXXXXXXXXXNDKRNLIRYDEDLF 274
            ++  +FP +LTEEE  LQ+KYAKL++KKKQL  +  PK    +   K+  EAKD+KEVAKKL+++G ++AI+ S++K EKSK       FKRS+G+ERKL+   R +SSYQPFSATHGPGGGFDTG +D  PE P      K K+LYE+FV  RDREER       +K +  +TVYV+G+ ++E++LR TF+  G+IVNISME++KNCGFIT++K +S ER+I++ + T V GVQLKVS ARRQ +IDPINDASSS+TWS+IAASHSQK SH DKR ++ YDED+F
Sbjct:   20 IMVLHFPNKLTEEEEALQSKYAKLRRKKKQLQDVNKPKQEPEQNKLKRPSEAKDAKEVAKKLMKSGAMKAIKNSLQK-EKSKDEKTIGSFKRSKGFERKLNGMDRAMSSYQPFSATHGPGGGFDTGGEDFEPEIPEALQKSKQKNLYENFVKERDREERGLKEDTQNKPRVGHTVYVYGYGITEDMLRTTFTQYGQIVNISMEVEKNCGFITYEKVESTERSIAEVNGTQVGGVQLKVSLARRQPVIDPINDASSSSTWSTIAASHSQKGSHRDKREMVTYDEDIF 874          

BLAST of EMLSAG00000006819 vs. C. finmarchicus
Match: gi|592785340|gb|GAXK01169228.1| (TSA: Calanus finmarchicus comp502_c1_seq3 transcribed RNA sequence)

HSP 1 Score: 45.4394 bits (106), Expect = 1.385e-4
Identity = 32/112 (28.57%), Postives = 56/112 (50.00%), Query Frame = 0

Query:  143 FVSARDREERKGDKAKSANTVYVHGFS--VSEELLRNTFSSVGKIVNISM-------------EI-------DKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQ 232
            FV  ++RE+  G+KAK    VY+  F   + +E L+  FS  GKI +  +             EI       +K  GF++F+  +SAE+A+ + +   + G  L V +A+++
Sbjct:  741 FVPRKEREKELGEKAKKFTNVYIKNFGEDLGDEKLKEVFSKFGKITSYKVVKEKDLAQHAEGAEIMADESGKNKGFGFVSFEDSESAEKAVDELNGVEMFGKTLYVGRAQKK 1076          

BLAST of EMLSAG00000006819 vs. C. finmarchicus
Match: gi|592785341|gb|GAXK01169227.1| (TSA: Calanus finmarchicus comp502_c1_seq2 transcribed RNA sequence)

HSP 1 Score: 45.4394 bits (106), Expect = 1.481e-4
Identity = 32/112 (28.57%), Postives = 56/112 (50.00%), Query Frame = 0

Query:  143 FVSARDREERKGDKAKSANTVYVHGFS--VSEELLRNTFSSVGKIVNISM-------------EI-------DKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQ 232
            FV  ++RE+  G+KAK    VY+  F   + +E L+  FS  GKI +  +             EI       +K  GF++F+  +SAE+A+ + +   + G  L V +A+++
Sbjct:  741 FVPRKEREKELGEKAKKFTNVYIKNFGEDLGDEKLKEVFSKFGKITSYKVVKEKDLAQHAEGAEIMADESGKNKGFGFVSFEDSESAEKAVDELNGVEMFGKTLYVGRAQKK 1076          

BLAST of EMLSAG00000006819 vs. C. finmarchicus
Match: gi|592785342|gb|GAXK01169226.1| (TSA: Calanus finmarchicus comp502_c1_seq1 transcribed RNA sequence)

HSP 1 Score: 45.4394 bits (106), Expect = 1.481e-4
Identity = 32/112 (28.57%), Postives = 56/112 (50.00%), Query Frame = 0

Query:  143 FVSARDREERKGDKAKSANTVYVHGFS--VSEELLRNTFSSVGKIVNISM-------------EI-------DKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQ 232
            FV  ++RE+  G+KAK    VY+  F   + +E L+  FS  GKI +  +             EI       +K  GF++F+  +SAE+A+ + +   + G  L V +A+++
Sbjct:  741 FVPRKEREKELGEKAKKFTNVYIKNFGEDLGDEKLKEVFSKFGKITSYKVVKEKDLAQHAEGAEIMADESGKNKGFGFVSFEDSESAEKAVDELNGVEMFGKTLYVGRAQKK 1076          

BLAST of EMLSAG00000006819 vs. C. finmarchicus
Match: gi|592823093|gb|GAXK01131475.1| (TSA: Calanus finmarchicus comp8986956_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 41.9726 bits (97), Expect = 2.692e-4
Identity = 23/69 (33.33%), Postives = 37/69 (53.62%), Query Frame = 0

Query:  168 FSVSEELLRNTFSSVGKIVNISMEIDKNCG------FITFDKEDSAERAISDYHETSVKGVQLKVSKAR 230
            +  +E+ +R+ F   GKI ++ +  D+  G      F+T+D E  AE AI        +G +LKV+KAR
Sbjct:   66 YRTTEDSVRSHFDKWGKITDVHLPRDRQTGDSRGFGFVTYDDERDAEDAIRAMDGVEFEGRRLKVNKAR 272          

BLAST of EMLSAG00000006819 vs. C. finmarchicus
Match: gi|592793275|gb|GAXK01161293.1| (TSA: Calanus finmarchicus comp35928_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 43.5134 bits (101), Expect = 5.616e-4
Identity = 19/58 (32.76%), Postives = 33/58 (56.90%), Query Frame = 0

Query:  175 LRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQ 232
            L++ F   G +++I +   K CG+  +  ++SAE+A++  H   V G +LKV  A  Q
Sbjct: 1612 LKDVFGRFGGLIDIYLLNGKTCGYAKYAGKESAEKAVTALHGQEVCGARLKVMPADPQ 1785          

BLAST of EMLSAG00000006819 vs. C. finmarchicus
Match: gi|592785127|gb|GAXK01169441.1| (TSA: Calanus finmarchicus comp432_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 40.4318 bits (93), Expect = 4.834e-3
Identity = 24/65 (36.92%), Postives = 39/65 (60.00%), Query Frame = 0

Query:  170 VSEELLRNTFSSVGKIVNISMEIDK------NCGFITFDKEDSAERAISDYHETSVKGVQLKVSK 228
            +S+E +++ F+  G+I  +    DK      N  FITF+KEDSA++ +     T+V GV+L+V K
Sbjct:  446 LSDEEIKSHFAQFGQIAQVEQPFDKTKNERKNFCFITFEKEDSAKQLLK-AGTTTVNGVELEVKK 637          

BLAST of EMLSAG00000006819 vs. C. finmarchicus
Match: gi|592892691|gb|GAXK01065684.1| (TSA: Calanus finmarchicus comp5828_c1_seq3 transcribed RNA sequence)

HSP 1 Score: 39.2762 bits (90), Expect = 6.121e-3
Identity = 20/55 (36.36%), Postives = 30/55 (54.55%), Query Frame = 0

Query:  158 KSANTVYVHGF--SVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERA 210
            K   T+Y  G    V E+ ++N F + G+I +I++   + C FI F K  SAE A
Sbjct:   64 KMITTLYCGGLDEGVGEQDIKNAFYAYGEIRSITLVAKQGCAFIQFTKRSSAELA 228          

BLAST of EMLSAG00000006819 vs. C. finmarchicus
Match: gi|592892687|gb|GAXK01065688.1| (TSA: Calanus finmarchicus comp5828_c2_seq2 transcribed RNA sequence)

HSP 1 Score: 39.2762 bits (90), Expect = 6.574e-3
Identity = 20/55 (36.36%), Postives = 30/55 (54.55%), Query Frame = 0

Query:  158 KSANTVYVHGF--SVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERA 210
            K   T+Y  G    V E+ ++N F + G+I +I++   + C FI F K  SAE A
Sbjct:  399 KMITTLYCGGLDEGVGEQDIKNAFYAYGEIRSITLVAKQGCAFIQFTKRSSAELA 563          

BLAST of EMLSAG00000006819 vs. C. finmarchicus
Match: gi|592892692|gb|GAXK01065683.1| (TSA: Calanus finmarchicus comp5828_c1_seq2 transcribed RNA sequence)

HSP 1 Score: 39.2762 bits (90), Expect = 7.818e-3
Identity = 20/55 (36.36%), Postives = 30/55 (54.55%), Query Frame = 0

Query:  158 KSANTVYVHGF--SVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERA 210
            K   T+Y  G    V E+ ++N F + G+I +I++   + C FI F K  SAE A
Sbjct:  343 KMITTLYCGGLDEGVGEQDIKNAFYAYGEIRSITLVAKQGCAFIQFTKRSSAELA 507          

BLAST of EMLSAG00000006819 vs. L. salmonis peptides
Match: EMLSAP00000006819 (pep:novel supercontig:LSalAtl2s:LSalAtl2s383:42049:42873:-1 gene:EMLSAG00000006819 transcript:EMLSAT00000006819 description:"augustus_masked-LSalAtl2s383-processed-gene-0.10")

HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF
Sbjct:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274          

BLAST of EMLSAG00000006819 vs. L. salmonis peptides
Match: EMLSAP00000000109 (pep:novel supercontig:LSalAtl2s:LSalAtl2s101:164948:165772:1 gene:EMLSAG00000000109 transcript:EMLSAT00000000109 description:"augustus_masked-LSalAtl2s101-processed-gene-1.12")

HSP 1 Score: 233.417 bits (594), Expect = 2.655e-75
Identity = 157/287 (54.70%), Postives = 202/287 (70.38%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEK-TYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREERKGDKAKS------ANTVYVHGFS-VSEELLRNTFSSVGKIVNISMEIDKN---CGFITFDKEDSAERAISDYHETSVKGVQLKVSKA-RRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRY-DEDLF 274
            M Y  F   L+EEE  LQAKYAKL+KKKK++AALK PKP ESEK + KK++EA D+KEVAK+L +TG L AIQKSVEKT+KS+GFKRSQGWERKL+D R LS+YQPFSATHGPGGGFDTG DDLNPEPP+     K        VS    +  K DK  S        T+YV+G S ++   L NTFS +G ++N+ +++DK     G ITF+K + AE+AISD+H+ +V+G++L+  K    +++ D  N+      WSSIAASHSQK SH DKR+L++Y +EDLF
Sbjct:    1 MGYLQFSSNLSEEERFLQAKYAKLRKKKKEVAALKTPKPIESEKISSKKIIEAVDAKEVAKRLSKTGALLAIQKSVEKTDKSRGFKRSQGWERKLADSRTLSAYQPFSATHGPGGGFDTGXDDLNPEPPLHNSSSK--------VSHHKHQRSKSDKRSSNVKLDEGATIYVYGNSLLTSHFLLNTFSHLGSVLNVFVDVDKGQGRGGSITFEKSELAEKAISDFHKATVEGIKLQCFKEPGEKVVWDTSNEP-----WSSIAASHSQKGSHKDKRDLVQYTEEDLF 274          

BLAST of EMLSAG00000006819 vs. SwissProt
Match: gi|38372288|sp|P92204.1|NELFE_DROME (RecName: Full=Negative elongation factor E)

HSP 1 Score: 218.009 bits (554), Expect = 3.963e-69
Identity = 137/288 (47.57%), Postives = 190/288 (65.97%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPS-ESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWER--KLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER-----------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEE MLQAKY KLKKKKK L A KAPKP  ES  T K+  +A+D++EVA+KLI++G + AIQK  ++ + S  FKR +G ER  + +    ++SYQPFS+T          ++ +  EP       + ++LY+ F + RDREER           + +K ++ NT++V G  V+E+ L+ TF+  G IVN+SMEI+K+ GF++F K +SA+RAI++ H  +V G+ L+V  ARRQ  I+PINDASSSA WSSIAAS SQK SH D R +++YDED  
Sbjct:    1 MVYIHFPNNLTEEEQMLQAKYQKLKKKKKALQAHKAPKPEPESSLTLKRPTDARDAREVARKLIKSGAIPAIQKQTKQDQTS--FKRPKGQERAKRSTSETTVASYQPFSSTQNDVAQETIISEIIKEEP-------RRQNLYQHFATERDREERGMPEKVPMDTAQPEKPRAGNTIFVSGNKVTEDFLKKTFNDYGTIVNVSMEIEKSRGFVSFAKPESADRAIAEIHGKNVNGINLQVQLARRQPQIEPINDASSSAVWSSIAASKSQKGSHKDHREMVQYDEDFL 279          

BLAST of EMLSAG00000006819 vs. SwissProt
Match: gi|38372380|sp|Q95ZE9.1|NELFE_DROVI (RecName: Full=Negative elongation factor E)

HSP 1 Score: 197.978 bits (502), Expect = 2.477e-61
Identity = 133/288 (46.18%), Postives = 185/288 (64.24%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPS-ESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWER--KLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER-----------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEE MLQAKY KLKKKKK L A KAPK   ES  T K+  +A+D++EVA+KLI++G + AIQK  +  +    FKR +G ER  + +    +++YQPFS+T          ++ +  EP       + ++LY+ F + R REER           + +K ++ NT++V G  V+EE L+ TF+  G IVN+SMEI+K+ GF++F K +SA+RAI++ H  SV G+ L+V  ARRQ  I PINDASSSA WSSIAAS SQK SH D R +++Y++D  
Sbjct:    1 MVYIHFPNNLTEEEHMLQAKYQKLKKKKKALQAHKAPKQEPESALTLKRPTDARDAREVARKLIKSGAIPAIQKQ-QTKQDQTSFKRPKGQERAKRSTSETTVAAYQPFSSTQNDVAQETIISEIIKEEP-------RRQNLYQHFATERVREERGLTEKVTLDTTQPEKPRAGNTIFVSGNKVTEEFLKKTFNDYGTIVNVSMEIEKSRGFVSFAKPESADRAIAEMHGKSVTGIVLQVQLARRQPQIVPINDASSSAVWSSIAASKSQKGSHKDLRQMVQYNDDFL 280          

BLAST of EMLSAG00000006819 vs. SwissProt
Match: gi|122146333|sp|Q0V898.1|NELFE_BOVIN (RecName: Full=Negative elongation factor E; Short=NELF-E; AltName: Full=RNA-binding protein RD)

HSP 1 Score: 105.531 bits (262), Expect = 4.032e-25
Identity = 51/126 (40.48%), Postives = 81/126 (64.29%), Query Frame = 0

Query:  149 REERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            R  RKG      NT+YV+G  ++  LLR  FS  G I+++SM+  +NC F+T++K +SA++A+++ + T V+ VQLKVS AR+Q ++D    A+  + W S+A  +S K  H DKR  I Y +D++
Sbjct:  250 RAPRKG------NTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVSIARKQPMLDA---ATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVY 366          

BLAST of EMLSAG00000006819 vs. SwissProt
Match: gi|38372876|sp|P19426.2|NELFE_MOUSE (RecName: Full=Negative elongation factor E; Short=NELF-E; AltName: Full=RNA-binding protein RD)

HSP 1 Score: 104.76 bits (260), Expect = 8.041e-25
Identity = 51/125 (40.80%), Postives = 80/125 (64.00%), Query Frame = 0

Query:  149 REERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDL 273
            R  RKG      NT+YV+G  ++  LLR  FS  G I+++SM+  +NC F+T++K +SA++A+++ + T V+ VQLKV+ AR+Q ++D    A+  + W S+A  +S K  H DKR  I Y +DL
Sbjct:  260 RAPRKG------NTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLD---AATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDL 375          

BLAST of EMLSAG00000006819 vs. SwissProt
Match: gi|1350554|sp|P18615.3|NELFE_HUMAN (RecName: Full=Negative elongation factor E; Short=NELF-E; AltName: Full=RNA-binding protein RD)

HSP 1 Score: 103.99 bits (258), Expect = 2.015e-24
Identity = 46/117 (39.32%), Postives = 78/117 (66.67%), Query Frame = 0

Query:  158 KSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            +  NT+YV+G  ++  LLR  FS  G I+++SM+  +NC F+T++K +SA++A+++ + T V+ VQLKV+ AR+Q ++D    A+  + W S+A  +S K  H DKR  I Y +D++
Sbjct:  259 RKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDA---ATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVY 372          

BLAST of EMLSAG00000006819 vs. SwissProt
Match: gi|75334880|sp|Q9LEB4.1|RBP45_NICPL (RecName: Full=Polyadenylate-binding protein RBP45; Short=Poly(A)-binding protein RBP45; AltName: Full=RNA-binding protein 45; Short=NplRBP45)

HSP 1 Score: 57.7658 bits (138), Expect = 2.486e-8
Identity = 27/86 (31.40%), Postives = 50/86 (58.14%), Query Frame = 0

Query:  147 RDREERKGDKAKSANTVYVHGF--SVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKAR 230
            ++ +  +G+   +  T++V G   +V+EE LR  FS  G++V++ +   K CGF+ F    SAE+A+S  + T + G  +++S  R
Sbjct:  268 QNPQATQGESDPNNTTIFVGGLDPTVAEEHLRQVFSPYGELVHVKIVAGKRCGFVQFGTRASAEQALSSLNGTQLGGQSIRLSWGR 353          

BLAST of EMLSAG00000006819 vs. SwissProt
Match: gi|206729905|sp|P31483.3|TIA1_HUMAN (RecName: Full=Nucleolysin TIA-1 isoform p40; AltName: Full=RNA-binding protein TIA-1; AltName: Full=T-cell-restricted intracellular antigen-1; Short=TIA-1; AltName: Full=p40-TIA-1)

HSP 1 Score: 56.6102 bits (135), Expect = 5.749e-8
Identity = 35/112 (31.25%), Postives = 58/112 (51.79%), Query Frame = 0

Query:  130 PPPPKKVKDLYESFVSARDREERKGDKAKSANTVYVHGFS--VSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQI-IIDPI 238
            PP PK     YES       +E     + S  TVY  G +  ++E+L+R TFS  G+I+ I +  DK   F+ F+  +SA  AI   + T+++G  +K    +  + +I+P+
Sbjct:  186 PPAPKST---YESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGKETLDMINPV 294          

BLAST of EMLSAG00000006819 vs. SwissProt
Match: gi|1729966|sp|P52912.1|TIA1_MOUSE (RecName: Full=Nucleolysin TIA-1; AltName: Full=RNA-binding protein TIA-1; AltName: Full=T-cell-restricted intracellular antigen-1; Short=TIA-1)

HSP 1 Score: 54.299 bits (129), Expect = 3.159e-7
Identity = 34/112 (30.36%), Postives = 57/112 (50.89%), Query Frame = 0

Query:  130 PPPPKKVKDLYESFVSARDREERKGDKAKSANTVYVHGFS--VSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQI-IIDPI 238
            PP PK     YES       +E     + +  TVY  G +  ++E+L+R TFS  G+I+ I +  DK   F+ F   +SA  AI   + T+++G  +K    +  + +I+P+
Sbjct:  186 PPAPKST---YESNTKQLSYDEVVSQSSPNNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFSSHESAAHAIVSVNGTTIEGHVVKCYWGKETLDMINPV 294          

BLAST of EMLSAG00000006819 vs. Select Arthropod Genomes
Match: gb|EFA00397.1| (Negative elongation factor E-like Protein [Tribolium castaneum])

HSP 1 Score: 256.914 bits (655), Expect = 7.312e-85
Identity = 155/284 (54.58%), Postives = 203/284 (71.48%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEK--TYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER--------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP   TEEELMLQAKY KLKKKKK L ALKAPKP E EK  T K+  EA+D++EVAKKLI++G + AI K  ++ E+  GFKR +G ERKL   +++S YQPFSA        D G D+   +PP      ++K+LY++F +ARDREER        K DK +  NT+YV G+ ++EE L+  FS++G IVNISMEI+KN GF+TFDK ++AERAI++   + V G+QLKV+ ARRQ  I+PINDASSSA W+++A+SHSQK +H DKRNL+ YD D+F
Sbjct:    1 MVYIHFPANFTEEELMLQAKYQKLKKKKKALQALKAPKP-EPEKPITPKRPAEARDAREVAKKLIKSGAIPAISKQQKQPEQ--GFKRPRGLERKLISDKIVSGYQPFSAVQ-----MDDGPDNPENKPP------RIKNLYDTFANARDREERGLTEKQREKPDKPRQGNTIYVSGYKITEEFLKKHFSTIGNIVNISMEIEKNRGFVTFDKVEAAERAIAEMDGSMVSGIQLKVTLARRQPQIEPINDASSSAAWATLASSHSQKGNHRDKRNLVSYD-DMF 269          

BLAST of EMLSAG00000006819 vs. Select Arthropod Genomes
Match: EEB20436.1 (Negative elongation factor E, putative [Pediculus humanus corporis])

HSP 1 Score: 256.914 bits (655), Expect = 9.697e-85
Identity = 157/287 (54.70%), Postives = 200/287 (69.69%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEKTY--KKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSD-PRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER----------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEELMLQAKY KLKKKKK L +LKAPKP   ++T   K+  E +D+KE AKKL+++G +    K+  + E++ GFKR  G ERKLS   R +S YQPFSAT            +  PE    P   KVK+LY+SFVSARDREER          K DK +  NT++V G+ ++E+LLR TF  +G IVN+SMEI+KN GF+TFDK D+AERAI++   + V GVQLKVS ARRQ +++ INDASSS+TWS+IAASHSQK +H DKR++I Y+EDLF
Sbjct:    1 MVYLHFPSNLTEEELMLQAKYTKLKKKKKALQSLKAPKPEPEKQTILPKRPAEGRDAKERAKKLVKSGAI-TTSKTPRRPEQA-GFKRPMGLERKLSGVERTVSGYQPFSATQ----------PEEAPETESRP---KVKNLYDSFVSARDREERGLGDKQKEQGKPDKPRQGNTIFVSGYKITEDLLRKTFQGMGNIVNVSMEIEKNRGFVTFDKMDAAERAINEMDGSMVSGVQLKVSLARRQPVVEAINDASSSSTWSTIAASHSQKGNHKDKRDVIVYEEDLF 272          

BLAST of EMLSAG00000006819 vs. Select Arthropod Genomes
Match: EFX68534.1 (hypothetical protein DAPPUDRAFT_301427 [Daphnia pulex])

HSP 1 Score: 238.039 bits (606), Expect = 2.524e-77
Identity = 151/285 (52.98%), Postives = 197/285 (69.12%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEKT--YKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDP-RVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREERKG--------DKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEELMLQ KY KLKKKKK L ALKAPKP E EKT   K+  EA+D++EVAKKL+++G + AI K  EKTE + GFKRS+G ERKLS   R  S +QPFS++ G      +  + +  E        KV +LYE+F+S+R+ +            +   S     V G+S++EE+L+  F S G +VNISMEI+KNCGF+TFDK  SA+RAIS+ + + V G+QLKVS ARRQ +I+PINDASSSATWS+IAA +SQK S+ DKR+L+ Y+E+LF
Sbjct:    1 MVYLHFPSNLTEEELMLQTKYQKLKKKKKALQALKAPKP-EPEKTPALKRPAEARDAREVAKKLLKSGAISAIPKPSEKTETT-GFKRSRGLERKLSSSDRAPSGFQPFSSSQG---SMVSNEEHVTHEARTK---SKVPNLYENFISSRESDAEPASISPTNPKETPTSPAISPVFGYSITEEILKKAFQSFGNVVNISMEIEKNCGFVTFDKTTSADRAISEMNGSMVSGIQLKVSLARRQPVIEPINDASSSATWSTIAARNSQKGSYKDKRDLVTYEEELF 277          

BLAST of EMLSAG00000006819 vs. Select Arthropod Genomes
Match: XP_394669.2 (PREDICTED: negative elongation factor E [Apis mellifera])

HSP 1 Score: 229.95 bits (585), Expect = 3.063e-74
Identity = 145/288 (50.35%), Postives = 196/288 (68.06%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEK---TYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKL-SDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREERKGD----------KAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEELMLQAKY KLK+KKK L  LKAPK  E+E+   T K+  EA+D++EVAKKLI++G + A  K+ ++ E+S  FKR +G  RKL S  + +SSYQPFSAT           ++  PE   P    +VKDLY+SFVSA+D E+R             K ++ NT++V G+ +SE+ L+  F + G I+NISME +KN GF+TFDK ++AERAIS+   + V  +QLKVS ARRQ II+ + DA+SS+ WS IAA++SQKS+H D+R+L  Y+EDLF
Sbjct:    4 MVYLHFPSNLTEEELMLQAKYNKLKRKKKALQDLKAPK-QETERVPQTPKRPTEARDAREVAKKLIKSGVITA-PKTPKRPEQS--FKRPRGLVRKLNSTEKTVSSYQPFSATQ---------MEEEEPETVRP----RVKDLYDSFVSAQDTEDRTSRDIQSPSKQEIKPRAGNTIFVCGYKISEDFLKKHFQTFGNIINISMEAEKNRGFVTFDKTEAAERAISEMDGSMVSSIQLKVSLARRQPIIESMTDATSSSMWSPIAANYSQKSAHKDRRDLKVYEEDLF 274          

BLAST of EMLSAG00000006819 vs. Select Arthropod Genomes
Match: AAF50394.1 (negative elongation factor E [Drosophila melanogaster])

HSP 1 Score: 218.009 bits (554), Expect = 1.921e-69
Identity = 137/288 (47.57%), Postives = 190/288 (65.97%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPS-ESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWER--KLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER-----------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEE MLQAKY KLKKKKK L A KAPKP  ES  T K+  +A+D++EVA+KLI++G + AIQK  ++ + S  FKR +G ER  + +    ++SYQPFS+T          ++ +  EP       + ++LY+ F + RDREER           + +K ++ NT++V G  V+E+ L+ TF+  G IVN+SMEI+K+ GF++F K +SA+RAI++ H  +V G+ L+V  ARRQ  I+PINDASSSA WSSIAAS SQK SH D R +++YDED  
Sbjct:    1 MVYIHFPNNLTEEEQMLQAKYQKLKKKKKALQAHKAPKPEPESSLTLKRPTDARDAREVARKLIKSGAIPAIQKQTKQDQTS--FKRPKGQERAKRSTSETTVASYQPFSSTQNDVAQETIISEIIKEEP-------RRQNLYQHFATERDREERGMPEKVPMDTAQPEKPRAGNTIFVSGNKVTEDFLKKTFNDYGTIVNVSMEIEKSRGFVSFAKPESADRAIAEIHGKNVNGINLQVQLARRQPQIEPINDASSSAVWSSIAASKSQKGSHKDHREMVQYDEDFL 279          

BLAST of EMLSAG00000006819 vs. Select Arthropod Genomes
Match: EAA10770.4 (AGAP006237-PA [Anopheles gambiae str. PEST])

HSP 1 Score: 208.764 bits (530), Expect = 7.866e-66
Identity = 137/284 (48.24%), Postives = 181/284 (63.73%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEKTY--KKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREERKG----------DKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDED 272
            MVY +FP  LTEEELMLQAKYAKL+KKKK L ALKAPK  E EK    K+  +A+D++EVA+KL+++G +QAIQK   K E S  FKR +G ERK + P +  + Q  SA+  P            P  P      +V++LY+ F + R++E++            DK +S NTVYV G  V+E  L+  FS  G+I+NISMEI+K  GFITF K +SA++AI+D H  +V G+QL+V  ARRQ  I+PINDA SSA WS++A + SQK  H D R L+ YD D
Sbjct:    1 MVYIHFPSNLTEEELMLQAKYAKLRKKKKALQALKAPK-QEPEKNLLPKRPADARDAREVARKLLKSGAIQAIQKPQPKQEGS--FKRPKGQERKRTQPEMSPAGQYQSASTTPAE---------TPNSPAEKETSRVQNLYQQFANEREKEDQSDKSKPGADASKDKPRSGNTVYVSGNKVTENFLKKHFSDFGEILNISMEIEKGRGFITFSKTESADKAIADLHSKTVGGIQLQVQLARRQPQINPINDAGSSAVWSALATNRSQKGKHKDNRELVCYDHD 272          

BLAST of EMLSAG00000006819 vs. Select Arthropod Genomes
Match: gb|KFM81525.1| (Negative elongation factor E, partial [Stegodyphus mimosarum])

HSP 1 Score: 207.608 bits (527), Expect = 3.295e-65
Identity = 129/300 (43.00%), Postives = 182/300 (60.67%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEKTYKKV-MEAK-DSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLS----DPRVLSSYQPFSATHG-----PGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSAR--------------DREERKGD----KAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDE 271
            MVY +FP +LTEEE ML+ KY KL++KKK L  L  PK         K  +EAK D+KEVAKKL+++G + AI+  VE  E+ +GFKR +  ERK +        ++ YQPFSA+H      P  G    +   +P     PP   VK LYESFVS+R               R ER  D    + +  NT+YV G+ V++E+L+  F  +G I+NISME+DKNCGF+TF+K + AE AI + + T+  GV+L VS ARRQ +I P+ + SSS++W+++A +  QK++H D RNLI Y++
Sbjct:    1 MVYLHFPSQLTEEEEMLKKKYEKLRRKKKALQNLNTPKQEPPPPKPLKRPLEAKPDAKEVAKKLLKSGAISAIK--VENKER-QGFKRPKALERKRNACDKTNSSVTGYQPFSASHSLEEEPPSLGSPNDSASDSPNSSGRPP---VKKLYESFVSSRKLEDEIKEDRARENQRRERDRDGRPERPRQGNTIYVRGYGVTQEILQKAFEPIGSIINISMEMDKNCGFVTFEKIEQAETAIKEVNGTTASGVRLNVSLARRQPLIPPLGEDSSSSSWATLAVNFPQKANHVDSRNLIVYED 294          

BLAST of EMLSAG00000006819 vs. Select Arthropod Genomes
Match: gb|KPM04776.1| (negative elongation factor E-like protein [Sarcoptes scabiei])

HSP 1 Score: 137.117 bits (344), Expect = 7.256e-38
Identity = 109/299 (36.45%), Postives = 170/299 (56.86%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESE-KTYKKVME-AKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERK-LSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARD---REERKGDKA--------KSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQ------IIIDPINDA-------SSSATWSSIAASHSQKSSHNDKRNLIRYDED 272
            M +   P +LTEEEL L+ KY KLK+KKK L  LK PK  +   +  K+  E   D+KEVAKKL+++G +QAI+   +K  +  GFKRS+  ERK  +       YQPF+ ++    G ++  + +  E  +   PK    L E   +AR+   RE+++  KA        +  NT+YVHG +++EE+LR  FS+ G I+NI+ E  +NCGF+T+D  ++A  AI + +   + G++LKVS ARRQ      + I+    A       S+S  WS++A++ S    ++  RN+I Y++D
Sbjct:    1 MTFVQLPSKLTEEELALKRKYQKLKRKKKLLQQLKTPKQEQPVVQPIKRAPENGTDAKEVAKKLLKSGKIQAIKVPDKKERQESGFKRSKAHERKRCTGTDKPGGYQPFNQSYSNDDGSES--ESIKSEKMLENNPK----LREIHSAAREEAFREKKRDGKANYVNRDDPQQGNTIYVHGENITEEILRLGFSTFGSILNINAETARNCGFVTYDSIETANMAIDEMNGKLLNGIRLKVSLARRQPSSLHSVTIEKPKSADLSNESLSTSEAWSAMASNVSNNVENSKNRNMISYEDD 293          

BLAST of EMLSAG00000006819 vs. Select Arthropod Genomes
Match: gb|EEC12699.1| (negative elongation factor E, putative, partial [Ixodes scapularis])

HSP 1 Score: 98.5969 bits (244), Expect = 4.695e-24
Identity = 85/235 (36.17%), Postives = 118/235 (50.21%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPK--PSESEKTYKKVMEAK-DSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERK--LSDPRVLSSYQPFSATHGP---------------------------------GGGFDTGAD---DLNPEPPIPPPPKKVKDLYESFVSARDREERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDK 194
            MVY +FP + TEEE MLQ KY KLK+KKK +  ++ PK  P + + T +KV E+K D+KEVAKKL+++G + AI+  VE  E+   FKRS+  ERK  L+D      YQP++A H                                   GG  D       D +PE   PPP                      ++ +  NT+YVHG  ++E+LLR+ FS  G I++ISME+DK
Sbjct:    1 MVYLHFPSQYTEEEEMLQKKYQKLKRKKKAMQQIRTPKQEPPQVQATLRKVSESKPDAKEVAKKLLKSGAISAIK--VETKER-HSFKRSKASERKRSLNDRPGGIGYQPYAANHPTEEESEATVSHSARFVYGLHRRSRTFSLWEWNRRGGNLDEDDPKHFDRDPEKEGPPP----------------------ERPRQGNTLYVHGVGLAEDLLRSAFSPFGPILSISMEVDK 210          

BLAST of EMLSAG00000006819 vs. Select Arthropod Genomes
Match: gb|EFA06678.1| (Polyadenylate-binding protein-like Protein [Tribolium castaneum])

HSP 1 Score: 52.373 bits (124), Expect = 5.986e-7
Identity = 34/99 (34.34%), Postives = 49/99 (49.49%), Query Frame = 0

Query:  130 PPPPKKVKDLYESFVSARDREERKGDKAKSANTVYVHGFS--VSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKV 226
            PPPPK  K    +     D  E     + +  TVY  GF+  ++E+L++ TFS  G I +I +  DK   FI F  ++SA  AI   H T + G  +K 
Sbjct:  176 PPPPKTEKAAQRAKQPTFD--EVYNQSSPTNCTVYCGGFTTGLTEDLMQKTFSQFGVIQDIRVFKDKGYAFIKFATKESATHAIETIHNTEINGQMVKC 272          

BLAST of EMLSAG00000006819 vs. nr
Match: gi|225717970|gb|ACO14831.1| (Negative elongation factor E [Caligus clemensi])

HSP 1 Score: 417.542 bits (1072), Expect = 3.909e-145
Identity = 219/296 (73.99%), Postives = 240/296 (81.08%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDPRVLSSYQPFSATHGPGGGFDTGADDLN----------------------PEPPIPPPPKKVKDLYESFVSARDREERKGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP +LTEEE  LQAKY KLKKKK+QLAALKA KPSE+EK  KKVMEA+DSKEVAKKL+RTG LQAIQKSVEK EKSKG KRSQGWERKL+DPRVLS YQPFSATHGPGGGFDTGADDLN                               KKVKDLYESFVSAR+REERK D  KSANTVYVHGF +SEELLR TF+ VGKIVNISME+DKNCGFITFDKE+SAE AISDYHET+  G+Q+KVSKARRQI +DPINDASSSATWSSIAASHSQK + +DKRNL+ YDE++F
Sbjct:    1 MVYISFPSQLTEEEQGLQAKYGKLKKKKRQLAALKASKPSEAEKVSKKVMEARDSKEVAKKLLRTGALQAIQKSVEKNEKSKGLKRSQGWERKLADPRVLSGYQPFSATHGPGGGFDTGADDLNPDPPPSAAPIPSNPPPSSNSTNNNTNNGNNGKKVKDLYESFVSARNREERKAD--KSANTVYVHGFDISEELLRKTFTPVGKIVNISMEVDKNCGFITFDKEESAECAISDYHETTTHGIQIKVSKARRQIQVDPINDASSSATWSSIAASHSQKGAFSDKRNLVIYDEEIF 294          

BLAST of EMLSAG00000006819 vs. nr
Match: gi|1058225176|gb|JAT16104.1| (hypothetical protein g.20571 [Graphocephala atropunctata] >gi|1058257462|gb|JAT32216.1| hypothetical protein g.20568 [Graphocephala atropunctata])

HSP 1 Score: 263.848 bits (673), Expect = 7.416e-85
Identity = 152/286 (53.15%), Postives = 201/286 (70.28%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPS-ESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDP-RVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER----------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEELMLQAKY KLK+KKK L ALKAP+P  E   T K+ MEA+++KEVAKKL+++G + AI ++ ++ E++ GFKR +G ERKLS   R +S YQPFSAT       D  ++ L P         +V DLYESFVSARDREER          + +K ++ NT++VHG  ++EE LR TF S G IVNISMEI+KN GF+TFDK ++AE AI++ + + V  +QLKV+ ARRQ II+P+NDASSS  WS++A++ SQK SH DKR+ + YD D+F
Sbjct:    1 MVYLHFPTNLTEEELMLQAKYQKLKRKKKALQALKAPRPEPERTPTPKRPMEAQNAKEVAKKLLKSGAITAIPRTPKRPEQA-GFKRPRGLERKLSGTDRTVSGYQPFSATQLD----DQESETLRP---------RVNDLYESFVSARDREERGLTDKPREEGRPEKPRTGNTIFVHGLKITEEFLRKTFQSCGNIVNISMEIEKNRGFVTFDKTEAAETAIAEINGSIVSNIQLKVTLARRQPIINPMNDASSSTAWSTLASNRSQKGSHKDKRDAVTYD-DMF 271          

BLAST of EMLSAG00000006819 vs. nr
Match: gi|1067091773|ref|XP_018007677.1| (PREDICTED: negative elongation factor E-like [Hyalella azteca] >gi|1067091775|ref|XP_018007678.1| PREDICTED: negative elongation factor E-like [Hyalella azteca])

HSP 1 Score: 262.307 bits (669), Expect = 8.489e-84
Identity = 150/317 (47.32%), Postives = 194/317 (61.20%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKP-SESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDP-RVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDR--------------------------------------EERKGDKA---KSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEE MLQ KY KL +K+K L  L+ PKP +E   T K+ MEAKD+KE+AKKLI++G ++  +K     E++  FKRS G ERKLS   +  S YQPFS +H              PE  +     KVK LY+SFVS R+R                                          GD     +S +T+Y+ G+++SEE+++  FSS G I+NISME+D NCGF+TF+K +SAE+AI++ + T V GVQLKVS ARRQ  ++PINDASSSATWS+IAASHSQK SHNDKR+L+RYDED F
Sbjct:    1 MVYLHFPSNLTEEEQMLQTKYQKLCRKRKALQQLRTPKPDAEQSPTNKRPMEAKDAKEMAKKLIKSGAIRVPEKLPSNNERTT-FKRSMGLERKLSAADKAPSGYQPFSTSH--------------PEEEVHESRLKVKHLYDSFVSDRERVGEKAEGRAAAAGGLEGEDPLSPLTPPRAGTAPGGALRPPLSGDAGVAPRSGHTIYIFGYNISEEVIKKNFSSFGSIINISMEVDNNCGFVTFEKVESAEKAIAEMNGTMVSGVQLKVSLARRQPPVEPINDASSSATWSTIAASHSQKGSHNDKRDLVRYDEDFF 302          

BLAST of EMLSAG00000006819 vs. nr
Match: gi|1058111175|gb|JAS59236.1| (hypothetical protein g.11465 [Cuerna arida])

HSP 1 Score: 258.07 bits (658), Expect = 1.463e-82
Identity = 152/286 (53.15%), Postives = 198/286 (69.23%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPS-ESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDP-RVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER----------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEELMLQAKY KLK+KKK L ALKAP+P  E   T K+ MEA+++KEVAKKL+++G + AI ++ ++ E++ GFKR +G ERKLS   R +S YQPFSAT           DD   E   P    +V DLYESFVSARDREER          + +K +S NT++VHG  ++E+ LR TF S G IVNISMEI+KN GF+TFDK ++A+ AI++ + + V  +QLKV+ ARRQ II+P NDA+SS  WSS+A++ SQK SH DKR  + YD D+F
Sbjct:    1 MVYLHFPTNLTEEELMLQAKYQKLKRKKKALQALKAPRPEPERTPTPKRPMEAQNAKEVAKKLLKSGAITAIPRTPKRPEQA-GFKRPRGLERKLSGTDRTVSGYQPFSATQ---------LDDQESETMRP----RVNDLYESFVSARDREERGLSDKPREEGRPEKPRSGNTIFVHGLKINEDFLRKTFQSCGNIVNISMEIEKNRGFVTFDKTEAADTAIAEINGSIVSNIQLKVTLARRQPIINPTNDATSSIAWSSLASNRSQKGSHKDKRESVIYD-DMF 271          

BLAST of EMLSAG00000006819 vs. nr
Match: gi|646714263|gb|KDR18301.1| (Negative elongation factor E [Zootermopsis nevadensis])

HSP 1 Score: 257.684 bits (657), Expect = 2.120e-82
Identity = 153/285 (53.68%), Postives = 201/285 (70.53%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPS--ESEKTYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDP-RVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER--------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEELMLQ KYAKLKKKKK L ALKAP+P    + +T K+  EA+D++EVA+KL+++G + AI K+ ++ E++ GFKR +G ERKLS   R +S YQPFSATH      D   D+            KVK+LY+SFVSARDREER        + DK +  NT++V G  ++E+ L+ TF S G IVN+++EI+KN GF+TFDK ++A++AI++ H   V GVQLKVS ARRQ +I PINDA+S+ATWS+IAASHSQK SH D+R LI Y+E  F
Sbjct:    1 MVYLHFPTNLTEEELMLQTKYAKLKKKKKALQALKAPRPEPERTPQTPKRPAEARDAREVARKLLKSGAITAITKTPKRPEQA-GFKRPRGLERKLSSTERTISGYQPFSATHPE----DMETDNR----------PKVKNLYDSFVSARDREERGLTEKRDGRADKPRQGNTIFVSGHEITEDFLKKTFHSYGNIVNVTVEIEKNHGFVTFDKMEAADQAITEMHHGMVSGVQLKVSLARRQPVIQPINDAASAATWSTIAASHSQKGSHRDRRKLIVYEEGFF 270          

BLAST of EMLSAG00000006819 vs. nr
Match: gi|91078286|ref|XP_971953.1| (PREDICTED: negative elongation factor E [Tribolium castaneum] >gi|270003949|gb|EFA00397.1| Negative elongation factor E-like Protein [Tribolium castaneum])

HSP 1 Score: 256.914 bits (655), Expect = 3.578e-82
Identity = 155/284 (54.58%), Postives = 203/284 (71.48%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEK--TYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER--------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP   TEEELMLQAKY KLKKKKK L ALKAPKP E EK  T K+  EA+D++EVAKKLI++G + AI K  ++ E+  GFKR +G ERKL   +++S YQPFSA        D G D+   +PP      ++K+LY++F +ARDREER        K DK +  NT+YV G+ ++EE L+  FS++G IVNISMEI+KN GF+TFDK ++AERAI++   + V G+QLKV+ ARRQ  I+PINDASSSA W+++A+SHSQK +H DKRNL+ YD D+F
Sbjct:    1 MVYIHFPANFTEEELMLQAKYQKLKKKKKALQALKAPKP-EPEKPITPKRPAEARDAREVAKKLIKSGAIPAISKQQKQPEQ--GFKRPRGLERKLISDKIVSGYQPFSAVQ-----MDDGPDNPENKPP------RIKNLYDTFANARDREERGLTEKQREKPDKPRQGNTIYVSGYKITEEFLKKHFSTIGNIVNISMEIEKNRGFVTFDKVEAAERAIAEMDGSMVSGIQLKVTLARRQPQIEPINDASSSAAWATLASSHSQKGNHRDKRNLVSYD-DMF 269          

BLAST of EMLSAG00000006819 vs. nr
Match: gi|242025524|ref|XP_002433174.1| (Negative elongation factor E, putative [Pediculus humanus corporis] >gi|212518715|gb|EEB20436.1| Negative elongation factor E, putative [Pediculus humanus corporis])

HSP 1 Score: 256.914 bits (655), Expect = 4.745e-82
Identity = 157/287 (54.70%), Postives = 200/287 (69.69%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEKTY--KKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSD-PRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER----------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEELMLQAKY KLKKKKK L +LKAPKP   ++T   K+  E +D+KE AKKL+++G +    K+  + E++ GFKR  G ERKLS   R +S YQPFSAT            +  PE    P   KVK+LY+SFVSARDREER          K DK +  NT++V G+ ++E+LLR TF  +G IVN+SMEI+KN GF+TFDK D+AERAI++   + V GVQLKVS ARRQ +++ INDASSS+TWS+IAASHSQK +H DKR++I Y+EDLF
Sbjct:    1 MVYLHFPSNLTEEELMLQAKYTKLKKKKKALQSLKAPKPEPEKQTILPKRPAEGRDAKERAKKLVKSGAI-TTSKTPRRPEQA-GFKRPMGLERKLSGVERTVSGYQPFSATQ----------PEEAPETESRP---KVKNLYDSFVSARDREERGLGDKQKEQGKPDKPRQGNTIFVSGYKITEDLLRKTFQGMGNIVNVSMEIEKNRGFVTFDKMDAAERAINEMDGSMVSGVQLKVSLARRQPVVEAINDASSSSTWSTIAASHSQKGNHKDKRDVIVYEEDLF 272          

BLAST of EMLSAG00000006819 vs. nr
Match: gi|1133421340|ref|XP_019866292.1| (PREDICTED: LOW QUALITY PROTEIN: negative elongation factor E [Aethina tumida])

HSP 1 Score: 254.988 bits (650), Expect = 1.833e-81
Identity = 148/284 (52.11%), Postives = 200/284 (70.42%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEK--TYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER--------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP   TEEE MLQ KY KLKKKKK L ALK+PKP E EK  T K+  EA+D++EVAKKLI++G +Q I K  +  E+  GFKR +G ERKL   +++S YQPFSA        + G D+   +PP      ++K+LY++F +ARDREER        K +K +S NT+YV G+ +SE+ L+  FS++G I+NISMEI+KN GF+TFDK ++AERAI++   + V G+QLKVS ARRQ  I+PIND +SSA W+++A+SHSQK +H DKR+L+ YD D+F
Sbjct:    1 MVYIHFPSNFTEEEQMLQTKYQKLKKKKKALQALKSPKP-EPEKPITPKRPAEARDAREVAKKLIKSGAIQPISKQQKAPEQ--GFKRPRGLERKLISDKIVSGYQPFSAVQ-----MEEGPDNPENKPP------RIKNLYDTFATARDREERGLTEKQREKPEKPRSGNTIYVSGYKISEDFLKKHFSTIGNIINISMEIEKNRGFVTFDKVEAAERAIAEMDGSMVSGIQLKVSLARRQPQIEPINDVTSSAAWATLASSHSQKGNHRDKRDLVSYD-DMF 269          

BLAST of EMLSAG00000006819 vs. nr
Match: gi|1080059255|ref|XP_018571504.1| (PREDICTED: negative elongation factor E isoform X2 [Anoplophora glabripennis])

HSP 1 Score: 252.677 bits (644), Expect = 1.729e-80
Identity = 154/284 (54.23%), Postives = 202/284 (71.13%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEK--TYKKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKLSDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER--------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP   TEEELMLQAKY KLKKKKK L ALKAPKP E EK    K+  EA+D++EVAKKLI++G + AI K  ++ E+  GFKR +G ERKL   +V+S YQPFSA        D G D+   +PP      ++K+LY++F +ARDREER        K +K +  NT+YV G+ ++EE L+  FS++G I+NISMEI+KN GFITFDK ++AERAIS+   + V G+QLKVS ARRQ  I+PINDA+SSA W+++A+SHSQK +H DKR+L+ YD D+F
Sbjct:    1 MVYIHFPANFTEEELMLQAKYQKLKKKKKALQALKAPKP-EPEKPIILKRPAEARDAREVAKKLIKSGVIPAISKQQKQPEQ--GFKRPRGLERKLISDKVVSGYQPFSAIQ-----MDDGPDNPENKPP------RIKNLYDTFANARDREERGLSEKQQQKSEKPRQGNTIYVSGYKITEEFLKKHFSTIGNIINISMEIEKNRGFITFDKVEAAERAISEMDGSMVSGIQLKVSLARRQPQIEPINDATSSAAWATLASSHSQKGNHRDKRDLVFYD-DIF 269          

BLAST of EMLSAG00000006819 vs. nr
Match: gi|998516845|ref|XP_015519228.1| (PREDICTED: negative elongation factor E [Neodiprion lecontei])

HSP 1 Score: 250.751 bits (639), Expect = 9.542e-80
Identity = 155/288 (53.82%), Postives = 194/288 (67.36%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEKTY---KKVMEAKDSKEVAKKLIRTGTLQAIQKSVEKTEKSKGFKRSQGWERKL-SDPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREER----------KGDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MVY +FP  LTEEELMLQAKY KLK+KKK L ALK PKP E E+     K+  EA+D++EVAKKLI++G + AI K+ ++ E+S  FKR +G ERKL S  + + SYQPFSAT           DD     P      +VKDLY+SFVSARD EER             K ++ NT++V G  +SE+ L+  F + G IVNISME +KN GFITFDK + AERAIS+   + V  +QLKVS ARRQ +IDPIND +SS+ WSS+AAS+SQKSSH D+R+L  YDEDLF
Sbjct:    1 MVYLHFPSNLTEEELMLQAKYNKLKRKKKALQALKTPKP-EPERIPQLPKRPTEARDAREVAKKLIKSGIIPAITKTQKRPEQS--FKRPRGLERKLNSTEKTICSYQPFSATQ-------PEEDDTEASRP------RVKDLYDSFVSARDHEERGLPDKQLGTRTEAKPRAGNTIFVCGHKISEDFLKKHFQTFGPIVNISMETEKNRGFITFDKAEVAERAISEMDGSMVSSIQLKVSLARRQPVIDPINDTTSSSMWSSVAASYSQKSSHKDRRDLKVYDEDLF 272          

BLAST of EMLSAG00000006819 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold334_size202906-snap-gene-0.19 (protein:Tk08961 transcript:maker-scaffold334_size202906-snap-gene-0.19-mRNA-1 annotation:"negative elongation factor e")

HSP 1 Score: 289.271 bits (739), Expect = 2.758e-98
Identity = 166/302 (54.97%), Postives = 222/302 (73.51%), Query Frame = 0

Query:    1 MVYFNFPLELTEEELMLQAKYAKLKKKKKQLAALKAPKPSESEKTYK----------------KVMEAKDSKEVAKKLIRTGTLQAIQKSVEK--TEKSKGFKRSQGWERKLS---DPRVLSSYQPFSATHGPGGGFDTGADDLNPEPPIPPPPKKVKDLYESFVSARDREERK-------GDKAKSANTVYVHGFSVSEELLRNTFSSVGKIVNISMEIDKNCGFITFDKEDSAERAISDYHETSVKGVQLKVSKARRQIIIDPINDASSSATWSSIAASHSQKSSHNDKRNLIRYDEDLF 274
            MV+ +FP +LTEEELML+AKY KLKK KK++A    P  ++ +   K                ++ EAKD+KEVAKKLIR+G LQ IQK++EK   EKS+GFKRSQG ERKL+   D R  + YQPFSATHGP GGFDTG +D+NPEP  PPP  KVK+LYE+FV AR+REER         +K +  +T+YV GF ++E+ L+  F++  KIVNISME++K+CGF+TF+K ++AE+AI++++   V G+Q++VS ARRQ +I+PINDASSS+TWS+IAASHSQK SH DKR+L+ YD+D+F
Sbjct:    1 MVWLSFPAKLTEEELMLKAKYEKLKKIKKKVALSLNPTKADDKSGDKGGLGGHSGLIGPLASHRIPEAKDAKEVAKKLIRSGALQTIQKTIEKDKQEKSRGFKRSQGLERKLTGSLDAR--AGYQPFSATHGPSGGFDTGGEDMNPEPMEPPP-PKVKNLYETFVHARNREERGLSDPEGLAEKPRQGHTIYVKGFGITEDFLQTIFANSSKIVNISMEVEKHCGFVTFEKPEAAEQAIAEHNGNMVHGIQIEVSMARRQPVIEPINDASSSSTWSTIAASHSQKGSHKDKRDLVAYDDDIF 299