BLAST of EMLSAG00000000227 vs. GO
Match: - (symbol:TRAF6 "Uncharacterized protein" species:9031 "Gallus gallus" [GO:0000122 "negative regulation of transcription from RNA polymerase II promoter" evidence=IEA] [GO:0001503 "ossification" evidence=IEA] [GO:0001843 "neural tube closure" evidence=IEA] [GO:0002218 "activation of innate immune response" evidence=TAS] [GO:0002224 "toll-like receptor signaling pathway" evidence=TAS] [GO:0002726 "positive regulation of T cell cytokine production" evidence=IEA] [GO:0002755 "MyD88-dependent toll-like receptor signaling pathway" evidence=TAS] [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0005811 "lipid particle" evidence=IEA] [GO:0005829 "cytosol" evidence=IEA] [GO:0007249 "I-kappaB kinase/NF-kappaB signaling" evidence=IEA] [GO:0007250 "activation of NF-kappaB-inducing kinase activity" evidence=TAS] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0009898 "cytoplasmic side of plasma membrane" evidence=IEA] [GO:0019886 "antigen processing and presentation of exogenous peptide antigen via MHC class II" evidence=IEA] [GO:0030316 "osteoclast differentiation" evidence=IEA] [GO:0031435 "mitogen-activated protein kinase kinase kinase binding" evidence=IEA] [GO:0031625 "ubiquitin protein ligase binding" evidence=IEA] [GO:0031666 "positive regulation of lipopolysaccharide-mediated signaling pathway" evidence=IEA] [GO:0031965 "nuclear membrane" evidence=IEA] [GO:0031996 "thioesterase binding" evidence=IEA] [GO:0032743 "positive regulation of interleukin-2 production" evidence=IEA] [GO:0034134 "toll-like receptor 2 signaling pathway" evidence=TAS] [GO:0034138 "toll-like receptor 3 signaling pathway" evidence=TAS] [GO:0034142 "toll-like receptor 4 signaling pathway" evidence=TAS] [GO:0034146 "toll-like receptor 5 signaling pathway" evidence=TAS] [GO:0034154 "toll-like receptor 7 signaling pathway" evidence=TAS] [GO:0035419 "activation of MAPK activity involved in innate immune response" evidence=TAS] [GO:0035631 "CD40 receptor complex" evidence=IEA] [GO:0035681 "toll-like receptor 15 signaling pathway" evidence=TAS] [GO:0035682 "toll-like receptor 21 signaling pathway" evidence=TAS] [GO:0042088 "T-helper 1 type immune response" evidence=IEA] [GO:0042102 "positive regulation of T cell proliferation" evidence=IEA] [GO:0042475 "odontogenesis of dentin-containing tooth" evidence=IEA] [GO:0042826 "histone deacetylase binding" evidence=IEA] [GO:0042981 "regulation of apoptotic process" evidence=IEA] [GO:0043011 "myeloid dendritic cell differentiation" evidence=IEA] [GO:0043123 "positive regulation of I-kappaB kinase/NF-kappaB signaling" evidence=IEA] [GO:0043422 "protein kinase B binding" evidence=IEA] [GO:0043507 "positive regulation of JUN kinase activity" evidence=IEA] [GO:0045084 "positive regulation of interleukin-12 biosynthetic process" evidence=IEA] [GO:0045087 "innate immune response" evidence=TAS] [GO:0045410 "positive regulation of interleukin-6 biosynthetic process" evidence=IEA] [GO:0045453 "bone resorption" evidence=IEA] [GO:0045672 "positive regulation of osteoclast differentiation" evidence=IEA] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IEA] [GO:0047485 "protein N-terminus binding" evidence=IEA] [GO:0048468 "cell development" evidence=IEA] [GO:0048471 "perinuclear region of cytoplasm" evidence=IEA] [GO:0050852 "T cell receptor signaling pathway" evidence=IEA] [GO:0051023 "regulation of immunoglobulin secretion" evidence=IEA] [GO:0051092 "positive regulation of NF-kappaB transcription factor activity" evidence=IEA] [GO:0070498 "interleukin-1-mediated signaling pathway" evidence=IEA] [GO:0070534 "protein K63-linked ubiquitination" evidence=IEA] [GO:2000679 "positive regulation of transcription regulatory region DNA binding" evidence=IEA] InterPro:IPR001293 InterPro:IPR001841 InterPro:IPR002083 InterPro:IPR008974 InterPro:IPR012227 InterPro:IPR027139 Pfam:PF00917 PIRSF:PIRSF015614 PROSITE:PS50089 PROSITE:PS50144 PROSITE:PS50145 SMART:SM00061 SMART:SM00184 Reactome:REACT_147795 Prosite:PS00518 GO:GO:0005829 GO:GO:0048471 GO:GO:0045087 GO:GO:0002755 GO:GO:0034134 GO:GO:0034138 GO:GO:0034142 GO:GO:0034146 GO:GO:0019886 GO:GO:0050852 GO:GO:0042088 GO:GO:0005811 GO:GO:0043123 GO:GO:0008270 GO:GO:0031965 GO:GO:0045944 GO:GO:0000122 GO:GO:0004871 GO:GO:0042981 GO:GO:0035419 GO:GO:0051092 Gene3D:3.30.40.10 InterPro:IPR013083 GO:GO:0042102 GO:GO:0001843 GO:GO:0042475 GO:GO:0045453 GO:GO:0004842 GO:GO:0001503 GO:GO:0007250 GO:GO:0009898 InterPro:IPR017907 GO:GO:0070534 GO:GO:0048468 GO:GO:0051865 GO:GO:0045672 GO:GO:0043507 GO:GO:0007249 Reactome:REACT_102124 SUPFAM:SSF49599 GO:GO:0043011 GO:GO:0034154 GO:GO:0035631 GO:GO:0030316 GO:GO:0032743 GO:GO:0031666 GO:GO:0051023 GO:GO:0070498 GO:GO:0045410 GO:GO:2000679 GO:GO:0045084 GO:GO:0035681 GO:GO:0035682 GO:GO:0002726 OrthoDB:EOG7966G5 TreeFam:TF321154 GeneTree:ENSGT00550000074359 CTD:7189 KO:K03175 PANTHER:PTHR10131:SF48 EMBL:AADN03004560 RefSeq:XP_004941602.1 RefSeq:XP_004941603.1 RefSeq:XP_004941604.1 RefSeq:XP_004941605.1 Ensembl:ENSGALT00000012888 GeneID:423163 KEGG:gga:423163 PRO:PR:E1C626 Uniprot:E1C626)

HSP 1 Score: 214.542 bits (545), Expect = 2.563e-61
Identity = 155/499 (31.06%), Postives = 238/499 (47.70%), Query Frame = 0

Query:    8 SGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT----------QERSSKKHENPSRSSDSNTCPSCGNVL--DDHE--------------------ITQGQGNRL--VCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDS----DLRLSGSNLHST-QKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVM------CSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVREIS----ENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            SGT   P  + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC  CI+  + + G  CP DN  L E  +FPD+ A REIL L + CP K  GC   + L  ++ H   C + T          Q+   K+H           CP+C   +  +D E                    I Q   N     CP A V C +++ GC  K+ R EL  H++  T  H+ ++   ++ I       S+T  + V       F+  + S  S    +      N   T Q+L   L R+   + +   ++E +N  + E LK+ +       +E EAQ   CNG ++WK+ +F  L K    +   V++S GFYT   GYK+CLR +I L + +    ++ +FVH M+G+ DS L WPF+G I L+ + +      +N  E ++  PE   AF+RP   RNP GFG+  F+ +  L    F+  ++TL+++ E+ T
Sbjct:   40 SGTGNPPSSFTEETQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKGCIVKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNK--GCCLKMELRHLEEHQLHCDFTTVECPQCQGAFQKNHLKEHMTQECPRRQVCCPNCATSMAYEDKELHDQTCPLANVFCEYCNTMLIRQQMPNHYDNDCPTAPVPCFYSAFGCPEKMQRNELARHMQEFTQVHMRMMAQSIQNI-------SVTATNPVPFINGLPFEPALFSHVSRAPYNCNPEVENFKETIQQLEGRLVRQDHQIRELIAKMETQNTHMAE-LKRTIRDLEGKITEMEAQ--QCNGIYIWKIENFSGLQKAQEEERPVVMHSPGFYTGKPGYKLCLRLHIQLPSAQRCANFISLFVHTMQGEYDSHLPWPFQGTIRLSILDQSEGPERQNHEEVMEAKPE-LLAFQRPTIHRNPKGFGYVTFMHLQTLKQRTFVK-DDTLLVRCEVLT 524          

BLAST of EMLSAG00000000227 vs. GO
Match: - (symbol:TRAF6 "TNF receptor-associated factor 6" species:9913 "Bos taurus" [GO:0000122 "negative regulation of transcription from RNA polymerase II promoter" evidence=IEA] [GO:0001503 "ossification" evidence=IEA] [GO:0001843 "neural tube closure" evidence=IEA] [GO:0002726 "positive regulation of T cell cytokine production" evidence=IEA] [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISS] [GO:0005811 "lipid particle" evidence=ISS] [GO:0005829 "cytosol" evidence=IEA] [GO:0005938 "cell cortex" evidence=IEA] [GO:0007249 "I-kappaB kinase/NF-kappaB signaling" evidence=IEA] [GO:0007250 "activation of NF-kappaB-inducing kinase activity" evidence=IEA] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0009898 "cytoplasmic side of plasma membrane" evidence=IEA] [GO:0019886 "antigen processing and presentation of exogenous peptide antigen via MHC class II" evidence=IEA] [GO:0030316 "osteoclast differentiation" evidence=IEA] [GO:0031435 "mitogen-activated protein kinase kinase kinase binding" evidence=IEA] [GO:0031625 "ubiquitin protein ligase binding" evidence=IEA] [GO:0031666 "positive regulation of lipopolysaccharide-mediated signaling pathway" evidence=IEA] [GO:0031965 "nuclear membrane" evidence=IEA] [GO:0031996 "thioesterase binding" evidence=IEA] [GO:0032743 "positive regulation of interleukin-2 production" evidence=IEA] [GO:0035631 "CD40 receptor complex" evidence=IEA] [GO:0042088 "T-helper 1 type immune response" evidence=IEA] [GO:0042102 "positive regulation of T cell proliferation" evidence=IEA] [GO:0042475 "odontogenesis of dentin-containing tooth" evidence=IEA] [GO:0042826 "histone deacetylase binding" evidence=IEA] [GO:0042981 "regulation of apoptotic process" evidence=IEA] [GO:0043011 "myeloid dendritic cell differentiation" evidence=IEA] [GO:0043123 "positive regulation of I-kappaB kinase/NF-kappaB signaling" evidence=ISS] [GO:0043422 "protein kinase B binding" evidence=IEA] [GO:0043507 "positive regulation of JUN kinase activity" evidence=IEA] [GO:0045084 "positive regulation of interleukin-12 biosynthetic process" evidence=IEA] [GO:0045410 "positive regulation of interleukin-6 biosynthetic process" evidence=IEA] [GO:0045453 "bone resorption" evidence=IEA] [GO:0045672 "positive regulation of osteoclast differentiation" evidence=IEA] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IEA] [GO:0047485 "protein N-terminus binding" evidence=IEA] [GO:0048468 "cell development" evidence=IEA] [GO:0048471 "perinuclear region of cytoplasm" evidence=IEA] [GO:0050852 "T cell receptor signaling pathway" evidence=IEA] [GO:0051023 "regulation of immunoglobulin secretion" evidence=IEA] [GO:0051092 "positive regulation of NF-kappaB transcription factor activity" evidence=ISS] [GO:0070498 "interleukin-1-mediated signaling pathway" evidence=IEA] [GO:0070534 "protein K63-linked ubiquitination" evidence=ISS] [GO:2000679 "positive regulation of transcription regulatory region DNA binding" evidence=IEA] InterPro:IPR001293 InterPro:IPR001841 InterPro:IPR002083 InterPro:IPR008974 InterPro:IPR012227 InterPro:IPR027139 Pfam:PF00917 PIRSF:PIRSF015614 PROSITE:PS50089 PROSITE:PS50144 PROSITE:PS50145 SMART:SM00061 SMART:SM00184 UniPathway:UPA00143 Prosite:PS00518 GO:GO:0005829 GO:GO:0005938 GO:GO:0048471 GO:GO:0019886 GO:GO:0050852 GO:GO:0042088 GO:GO:0005811 GO:GO:0043123 GO:GO:0008270 GO:GO:0031965 GO:GO:0045944 GO:GO:0000122 GO:GO:0004871 GO:GO:0042981 GO:GO:0051092 Gene3D:3.30.40.10 InterPro:IPR013083 GO:GO:0042102 GO:GO:0001843 GO:GO:0042475 GO:GO:0045453 GO:GO:0004842 GO:GO:0001503 GO:GO:0007250 GO:GO:0009898 InterPro:IPR017907 GO:GO:0070534 GO:GO:0048468 GO:GO:0051865 GO:GO:0045672 GO:GO:0043507 GO:GO:0007249 SUPFAM:SSF49599 GO:GO:0043011 GO:GO:0035631 GO:GO:0030316 GO:GO:0032743 GO:GO:0031666 GO:GO:0051023 GO:GO:0070498 GO:GO:0045410 GO:GO:2000679 GO:GO:0045084 GO:GO:0002726 OrthoDB:EOG7966G5 TreeFam:TF321154 GeneTree:ENSGT00550000074359 EMBL:DQ319074 EMBL:DQ407276 EMBL:BC102522 RefSeq:NP_001029833.1 RefSeq:XP_005216441.1 RefSeq:XP_005216442.1 UniGene:Bt.89201 ProteinModelPortal:Q3ZCC3 SMR:Q3ZCC3 PRIDE:Q3ZCC3 Ensembl:ENSBTAT00000021630 GeneID:539124 KEGG:bta:539124 CTD:7189 eggNOG:NOG289765 HOGENOM:HOG000006625 HOVERGEN:HBG060248 InParanoid:Q3ZCC3 KO:K03175 OMA:NFQETIH NextBio:20877796 PANTHER:PTHR10131:SF48 Uniprot:Q3ZCC3)

HSP 1 Score: 214.542 bits (545), Expect = 2.684e-61
Identity = 158/504 (31.35%), Postives = 240/504 (47.62%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT------QERSSKKHEN-------PSRSSDSNTCPSCGNVLDDHEITQ----------------------GQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNE---MLQEKLKKVM------CSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVRE----ISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D  +G + T  +   + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CII  + + G  CP DN  L E  +FPD+ A REIL LM+ CP  + GC   + L  ++ H   C++        Q    K H N       P R      C +     +D EI +                           L CP A V C F++ GC  K+ R  L  H++  T  H       +R + Q   T SL   + V +     +     +R S  R   S +H+ Q+ I+ L  R+V  + +  +L  + E   M   +LK+ +       +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L + +    Y+ +FVH M+G+ DS L WPF+G I LT + +    + +N  E +   PE   AF+RP   RNP GFG+  F+ + AL    FI  ++TL+++ E+ T
Sbjct:   35 DSVSGTASTVTLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCP--NEGCLHKMELRHLEEHQAHCEFALMSCPQCQRPFQKCHLNIHILKECPRRQVPCENC-AVSMAFEDKEIHEQNCPLANVICEYCNTMLIREQMPNHYDLDCPTAPVPCTFSAFGCHEKMQRNHLARHLQENTQSH-------MRMMAQAVQTLSLAV-APVPQCTMPLYDSVPPTRPSSGR--HSEVHNFQETIQQLEGRLVRQDHQIRELTAKMETQSMYVNELKRTIRTLEDKVAEIEAQ--QCNGIYIWKIGNFGMHLKSQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPSAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAAVRQNHEEIMDAKPE-LLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIK-DDTLLVRCEVST 521          

BLAST of EMLSAG00000000227 vs. GO
Match: - (symbol:TRAF6 "TNF receptor-associated factor 6" species:9606 "Homo sapiens" [GO:0000122 "negative regulation of transcription from RNA polymerase II promoter" evidence=IMP] [GO:0000187 "activation of MAPK activity" evidence=TAS] [GO:0000209 "protein polyubiquitination" evidence=IDA] [GO:0001503 "ossification" evidence=IEA] [GO:0001843 "neural tube closure" evidence=IEA] [GO:0002224 "toll-like receptor signaling pathway" evidence=TAS] [GO:0002726 "positive regulation of T cell cytokine production" evidence=IMP] [GO:0002755 "MyD88-dependent toll-like receptor signaling pathway" evidence=TAS] [GO:0002756 "MyD88-independent toll-like receptor signaling pathway" evidence=TAS] [GO:0004842 "ubiquitin-protein ligase activity" evidence=EXP;IDA;TAS] [GO:0005515 "protein binding" evidence=IPI] [GO:0005634 "nucleus" evidence=IDA] [GO:0005730 "nucleolus" evidence=IDA] [GO:0005737 "cytoplasm" evidence=IDA] [GO:0005739 "mitochondrion" evidence=IDA] [GO:0005811 "lipid particle" evidence=ISS] [GO:0005829 "cytosol" evidence=TAS] [GO:0005886 "plasma membrane" evidence=IDA;TAS] [GO:0005938 "cell cortex" evidence=IEA] [GO:0006461 "protein complex assembly" evidence=IEA] [GO:0007249 "I-kappaB kinase/NF-kappaB signaling" evidence=TAS] [GO:0007250 "activation of NF-kappaB-inducing kinase activity" evidence=IMP] [GO:0007254 "JNK cascade" evidence=TAS] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0009898 "cytoplasmic side of plasma membrane" evidence=ISS] [GO:0010008 "endosome membrane" evidence=TAS] [GO:0019886 "antigen processing and presentation of exogenous peptide antigen via MHC class II" evidence=IEA] [GO:0019901 "protein kinase binding" evidence=IPI] [GO:0030316 "osteoclast differentiation" evidence=IEA] [GO:0031293 "membrane protein intracellular domain proteolysis" evidence=TAS] [GO:0031398 "positive regulation of protein ubiquitination" evidence=NAS] [GO:0031435 "mitogen-activated protein kinase kinase kinase binding" evidence=IPI] [GO:0031625 "ubiquitin protein ligase binding" evidence=IPI] [GO:0031666 "positive regulation of lipopolysaccharide-mediated signaling pathway" evidence=IEA] [GO:0031996 "thioesterase binding" evidence=IPI] [GO:0032147 "activation of protein kinase activity" evidence=IDA] [GO:0032743 "positive regulation of interleukin-2 production" evidence=IMP] [GO:0034134 "toll-like receptor 2 signaling pathway" evidence=TAS] [GO:0034138 "toll-like receptor 3 signaling pathway" evidence=TAS] [GO:0034142 "toll-like receptor 4 signaling pathway" evidence=TAS] [GO:0034146 "toll-like receptor 5 signaling pathway" evidence=TAS] [GO:0034162 "toll-like receptor 9 signaling pathway" evidence=TAS] [GO:0034166 "toll-like receptor 10 signaling pathway" evidence=TAS] [GO:0035631 "CD40 receptor complex" evidence=ISS] [GO:0035666 "TRIF-dependent toll-like receptor signaling pathway" evidence=TAS] [GO:0035872 "nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway" evidence=TAS] [GO:0038095 "Fc-epsilon receptor signaling pathway" evidence=TAS] [GO:0038123 "toll-like receptor TLR1:TLR2 signaling pathway" evidence=TAS] [GO:0038124 "toll-like receptor TLR6:TLR2 signaling pathway" evidence=TAS] [GO:0042088 "T-helper 1 type immune response" evidence=IEA] [GO:0042102 "positive regulation of T cell proliferation" evidence=IEA] [GO:0042475 "odontogenesis of dentin-containing tooth" evidence=IEA] [GO:0042826 "histone deacetylase binding" evidence=IPI] [GO:0043011 "myeloid dendritic cell differentiation" evidence=IEA] [GO:0043065 "positive regulation of apoptotic process" evidence=TAS] [GO:0043066 "negative regulation of apoptotic process" evidence=TAS] [GO:0043123 "positive regulation of I-kappaB kinase/NF-kappaB signaling" evidence=IDA;TAS] [GO:0043422 "protein kinase B binding" evidence=IPI] [GO:0043507 "positive regulation of JUN kinase activity" evidence=NAS;IDA] [GO:0045084 "positive regulation of interleukin-12 biosynthetic process" evidence=IEA] [GO:0045087 "innate immune response" evidence=TAS] [GO:0045410 "positive regulation of interleukin-6 biosynthetic process" evidence=IEA] [GO:0045453 "bone resorption" evidence=IEA] [GO:0045672 "positive regulation of osteoclast differentiation" evidence=IDA] [GO:0045892 "negative regulation of transcription, DNA-templated" evidence=IMP] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=NAS;IDA] [GO:0047485 "protein N-terminus binding" evidence=IPI] [GO:0048011 "neurotrophin TRK receptor signaling pathway" evidence=TAS] [GO:0048468 "cell development" evidence=IEA] [GO:0048471 "perinuclear region of cytoplasm" evidence=IDA] [GO:0048661 "positive regulation of smooth muscle cell proliferation" evidence=IEA] [GO:0050852 "T cell receptor signaling pathway" evidence=IMP;TAS] [GO:0050870 "positive regulation of T cell activation" evidence=IC] [GO:0051023 "regulation of immunoglobulin secretion" evidence=IEA] [GO:0051091 "positive regulation of sequence-specific DNA binding transcription factor activity" evidence=IMP] [GO:0051092 "positive regulation of NF-kappaB transcription factor activity" evidence=IMP;IDA;TAS] [GO:0051403 "stress-activated MAPK cascade" evidence=TAS] [GO:0051865 "protein autoubiquitination" evidence=IDA;TAS] [GO:0070423 "nucleotide-binding oligomerization domain containing signaling pathway" evidence=TAS] [GO:0070498 "interleukin-1-mediated signaling pathway" evidence=IEA] [GO:0070534 "protein K63-linked ubiquitination" evidence=IGI;IDA] [GO:0070555 "response to interleukin-1" evidence=IDA] [GO:0097190 "apoptotic signaling pathway" evidence=TAS] [GO:2000679 "positive regulation of transcription regulatory region DNA binding" evidence=IDA] Reactome:REACT_6782 InterPro:IPR001293 InterPro:IPR001841 InterPro:IPR002083 InterPro:IPR008974 InterPro:IPR012227 InterPro:IPR027139 Pfam:PF00917 PIRSF:PIRSF015614 PROSITE:PS50089 PROSITE:PS50144 PROSITE:PS50145 SMART:SM00061 SMART:SM00184 UniPathway:UPA00143 Prosite:PS00518 GO:GO:0005829 GO:GO:0005938 GO:GO:0048471 Reactome:REACT_111102 Reactome:REACT_6900 GO:GO:0038095 GO:GO:0045087 GO:GO:0048011 GO:GO:0043066 EMBL:CH471064 GO:GO:0002755 GO:GO:0034166 GO:GO:0034134 GO:GO:0034138 GO:GO:0034142 GO:GO:0034146 GO:GO:0034162 GO:GO:0038123 GO:GO:0038124 GO:GO:0035666 GO:GO:0006461 GO:GO:0019886 GO:GO:0050852 GO:GO:0042088 GO:GO:0007254 GO:GO:0005811 GO:GO:0043123 GO:GO:0008270 GO:GO:0031965 GO:GO:0048661 GO:GO:0045944 GO:GO:0097190 GO:GO:0000187 GO:GO:0043065 GO:GO:0010008 GO:GO:0000122 GO:GO:0004871 GO:GO:0031293 GO:GO:0051092 Gene3D:3.30.40.10 InterPro:IPR013083 GO:GO:0042102 GO:GO:0001843 GO:GO:0042475 GO:GO:0045453 GO:GO:0031398 GO:GO:0070555 GO:GO:0004842 GO:GO:0001503 GO:GO:0007250 GO:GO:0009898 InterPro:IPR017907 GO:GO:0070534 GO:GO:0048468 GO:GO:0051865 GO:GO:0045672 GO:GO:0043507 GO:GO:0007249 SUPFAM:SSF49599 GO:GO:0043011 GO:GO:0050870 GO:GO:0035631 GO:GO:0030316 GO:GO:0070423 GO:GO:0032743 GO:GO:0031666 GO:GO:0051023 Orphanet:1810 GO:GO:0070498 GO:GO:0045410 GO:GO:2000679 GO:GO:0045084 GO:GO:0002726 EMBL:AC009656 PDB:1LB5 PDBsum:1LB5 PDB:1LB6 PDBsum:1LB6 OrthoDB:EOG7966G5 TreeFam:TF321154 CTD:7189 eggNOG:NOG289765 HOGENOM:HOG000006625 HOVERGEN:HBG060248 KO:K03175 OMA:NFQETIH PANTHER:PTHR10131:SF48 EMBL:U78798 EMBL:AY228337 EMBL:AK292978 EMBL:AC061999 EMBL:BC031052 PIR:S71821 RefSeq:NP_004611.1 RefSeq:NP_665802.1 UniGene:Hs.444172 UniGene:Hs.591983 PDB:1LB4 PDB:2ECI PDB:2JMD PDB:3HCS PDB:3HCT PDB:3HCU PDBsum:1LB4 PDBsum:2ECI PDBsum:2JMD PDBsum:3HCS PDBsum:3HCT PDBsum:3HCU ProteinModelPortal:Q9Y4K3 SMR:Q9Y4K3 BioGrid:113041 DIP:DIP-27515N IntAct:Q9Y4K3 MINT:MINT-88585 STRING:9606.ENSP00000316840 PhosphoSite:Q9Y4K3 DMDM:30580642 PaxDb:Q9Y4K3 PRIDE:Q9Y4K3 DNASU:7189 Ensembl:ENST00000348124 Ensembl:ENST00000526995 GeneID:7189 KEGG:hsa:7189 UCSC:uc001mwq.2 GeneCards:GC11M036467 HGNC:HGNC:12036 HPA:CAB004605 HPA:HPA019805 HPA:HPA020599 MIM:602355 neXtProt:NX_Q9Y4K3 PharmGKB:PA36713 InParanoid:Q9Y4K3 PhylomeDB:Q9Y4K3 SignaLink:Q9Y4K3 ChiTaRS:Traf6 EvolutionaryTrace:Q9Y4K3 GeneWiki:TRAF6 GenomeRNAi:7189 NextBio:28192 PRO:PR:Q9Y4K3 Bgee:Q9Y4K3 CleanEx:HS_TRAF6 Genevestigator:Q9Y4K3 Uniprot:Q9Y4K3)

HSP 1 Score: 213.001 bits (541), Expect = 7.882e-61
Identity = 151/496 (30.44%), Postives = 231/496 (46.57%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT------QERSSKKHEN-------PSRSSDSNTCPSCGNVLDDHEI----------------------TQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSR-DSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVRE----ISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D   G + T  +   + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CII  + + G  CP DN  L E  +FPD+ A REIL LM+ CP  + GC   + L  +++H   C++        Q    K H N       P R    + C +     +D EI                             L CP A + C F++ GC  K+ R  L  H++  T  H+ +L   +  +    D+  ++   +  ET      EG L R D  +R   + + +    + +L R + +LE K                    +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L   +    Y+ +FVH M+G+ DS L WPF+G I LT + +    + +N  E +   PE   AF+RP   RNP GFG+  F+ + AL    FI  ++TL+++ E+ T
Sbjct:   34 DSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCP--NEGCLHKMELRHLEDHQAHCEFALMDCPQCQRPFQKFHINIHILKDCPRRQVSCDNCAA-SMAFEDKEIHDQNCPLANVICEYCNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQ--LEGRLVRQDHQIRELTAKMETQSMYVSELKRTIRTLEDK-------------------VAEIEAQ--QCNGIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPE-LLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIK-DDTLLVRCEVST 501          

BLAST of EMLSAG00000000227 vs. GO
Match: - (symbol:Traf6 "TNF receptor-associated factor 6" species:10090 "Mus musculus" [GO:0000122 "negative regulation of transcription from RNA polymerase II promoter" evidence=ISO] [GO:0000209 "protein polyubiquitination" evidence=ISO] [GO:0001503 "ossification" evidence=IMP] [GO:0001843 "neural tube closure" evidence=IMP] [GO:0002376 "immune system process" evidence=IEA] [GO:0002726 "positive regulation of T cell cytokine production" evidence=ISO] [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISO;IMP;IDA] [GO:0004871 "signal transducer activity" evidence=TAS] [GO:0005515 "protein binding" evidence=IPI] [GO:0005622 "intracellular" evidence=IMP] [GO:0005634 "nucleus" evidence=ISO] [GO:0005730 "nucleolus" evidence=ISO] [GO:0005737 "cytoplasm" evidence=ISO] [GO:0005739 "mitochondrion" evidence=ISO] [GO:0005811 "lipid particle" evidence=IDA] [GO:0005829 "cytosol" evidence=IDA] [GO:0005886 "plasma membrane" evidence=ISO] [GO:0006461 "protein complex assembly" evidence=ISO] [GO:0006955 "immune response" evidence=IMP] [GO:0007165 "signal transduction" evidence=IDA;TAS] [GO:0007249 "I-kappaB kinase/NF-kappaB signaling" evidence=IMP] [GO:0007250 "activation of NF-kappaB-inducing kinase activity" evidence=ISO] [GO:0007254 "JNK cascade" evidence=ISO] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0009887 "organ morphogenesis" evidence=IMP] [GO:0009898 "cytoplasmic side of plasma membrane" evidence=IDA] [GO:0016567 "protein ubiquitination" evidence=IGI;IMP;IDA] [GO:0016874 "ligase activity" evidence=IEA] [GO:0019221 "cytokine-mediated signaling pathway" evidence=ISO] [GO:0019886 "antigen processing and presentation of exogenous peptide antigen via MHC class II" evidence=IMP] [GO:0019901 "protein kinase binding" evidence=ISO] [GO:0030316 "osteoclast differentiation" evidence=IMP] [GO:0031435 "mitogen-activated protein kinase kinase kinase binding" evidence=ISO] [GO:0031624 "ubiquitin conjugating enzyme binding" evidence=ISO] [GO:0031625 "ubiquitin protein ligase binding" evidence=ISO] [GO:0031666 "positive regulation of lipopolysaccharide-mediated signaling pathway" evidence=IMP] [GO:0031996 "thioesterase binding" evidence=ISO] [GO:0032147 "activation of protein kinase activity" evidence=ISO] [GO:0032743 "positive regulation of interleukin-2 production" evidence=ISO] [GO:0035631 "CD40 receptor complex" evidence=IDA] [GO:0042088 "T-helper 1 type immune response" evidence=IMP] [GO:0042102 "positive regulation of T cell proliferation" evidence=IMP] [GO:0042475 "odontogenesis of dentin-containing tooth" evidence=IMP] [GO:0042826 "histone deacetylase binding" evidence=ISO] [GO:0042981 "regulation of apoptotic process" evidence=IEA] [GO:0043011 "myeloid dendritic cell differentiation" evidence=IMP] [GO:0043123 "positive regulation of I-kappaB kinase/NF-kappaB signaling" evidence=IGI;ISO;IMP] [GO:0043234 "protein complex" evidence=ISO] [GO:0043422 "protein kinase B binding" evidence=ISO] [GO:0043507 "positive regulation of JUN kinase activity" evidence=ISO] [GO:0045084 "positive regulation of interleukin-12 biosynthetic process" evidence=IMP] [GO:0045410 "positive regulation of interleukin-6 biosynthetic process" evidence=IMP] [GO:0045453 "bone resorption" evidence=IMP] [GO:0045672 "positive regulation of osteoclast differentiation" evidence=ISO] [GO:0045892 "negative regulation of transcription, DNA-templated" evidence=ISO] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=ISO] [GO:0046849 "bone remodeling" evidence=IMP] [GO:0046872 "metal ion binding" evidence=IEA] [GO:0047485 "protein N-terminus binding" evidence=ISO] [GO:0048468 "cell development" evidence=IMP] [GO:0048471 "perinuclear region of cytoplasm" evidence=ISO] [GO:0048661 "positive regulation of smooth muscle cell proliferation" evidence=ISO] [GO:0050852 "T cell receptor signaling pathway" evidence=ISO] [GO:0051023 "regulation of immunoglobulin secretion" evidence=IDA] [GO:0051091 "positive regulation of sequence-specific DNA binding transcription factor activity" evidence=ISO] [GO:0051092 "positive regulation of NF-kappaB transcription factor activity" evidence=ISO;IMP] [GO:0051865 "protein autoubiquitination" evidence=ISO;TAS] [GO:0070498 "interleukin-1-mediated signaling pathway" evidence=ISO;IMP] [GO:0070534 "protein K63-linked ubiquitination" evidence=ISO;IDA] [GO:0070555 "response to interleukin-1" evidence=ISO] [GO:0071222 "cellular response to lipopolysaccharide" evidence=ISO] [GO:2000679 "positive regulation of transcription regulatory region DNA binding" evidence=ISO] InterPro:IPR001293 InterPro:IPR001841 InterPro:IPR002083 InterPro:IPR008974 InterPro:IPR012227 InterPro:IPR027139 Pfam:PF00917 PIRSF:PIRSF015614 PROSITE:PS50089 PROSITE:PS50144 PROSITE:PS50145 SMART:SM00061 SMART:SM00184 UniPathway:UPA00143 MGI:MGI:108072 Prosite:PS00518 GO:GO:0005829 GO:GO:0005938 GO:GO:0048471 GO:GO:0006461 GO:GO:0019886 GO:GO:0050852 GO:GO:0042088 GO:GO:0007254 GO:GO:0005811 GO:GO:0043123 GO:GO:0008270 GO:GO:0031965 GO:GO:0048661 GO:GO:0045944 Reactome:REACT_188257 EMBL:CH466519 GO:GO:0000122 GO:GO:0004871 GO:GO:0042981 Reactome:REACT_98458 GO:GO:0051092 Gene3D:3.30.40.10 InterPro:IPR013083 GO:GO:0042102 GO:GO:0001843 GO:GO:0042475 GO:GO:0045453 GO:GO:0004842 GO:GO:0001503 GO:GO:0007250 GO:GO:0009898 InterPro:IPR017907 GO:GO:0070534 GO:GO:0048468 GO:GO:0051865 GO:GO:0045672 GO:GO:0043507 GO:GO:0007249 SUPFAM:SSF49599 GO:GO:0043011 GO:GO:0035631 GO:GO:0030316 GO:GO:0032743 GO:GO:0031666 GO:GO:0051023 GO:GO:0070498 GO:GO:0045410 GO:GO:2000679 GO:GO:0045084 GO:GO:0002726 EMBL:AL929571 OrthoDB:EOG7966G5 TreeFam:TF321154 GeneTree:ENSGT00550000074359 CTD:7189 eggNOG:NOG289765 HOGENOM:HOG000006625 HOVERGEN:HBG060248 KO:K03175 OMA:NFQETIH PANTHER:PTHR10131:SF48 EMBL:D84655 EMBL:AK041172 EMBL:AK155434 EMBL:BC060705 RefSeq:NP_033450.2 RefSeq:XP_006499219.1 UniGene:Mm.292729 ProteinModelPortal:P70196 SMR:P70196 BioGrid:204307 DIP:DIP-29812N IntAct:P70196 MINT:MINT-252449 STRING:10090.ENSMUSP00000004949 PhosphoSite:P70196 PaxDb:P70196 PRIDE:P70196 Ensembl:ENSMUST00000004949 GeneID:22034 KEGG:mmu:22034 UCSC:uc008lhl.1 UCSC:uc008lhm.1 InParanoid:Q6P9M0 NextBio:301798 PRO:PR:P70196 Bgee:P70196 CleanEx:MM_TRAF6 Genevestigator:P70196 Uniprot:P70196)

HSP 1 Score: 212.231 bits (539), Expect = 1.623e-60
Identity = 146/503 (29.03%), Postives = 230/503 (45.73%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKY------------------------------------ITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGI-------LSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVRE----ISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D  +G + T  +   + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CII  + + G  CP DN  L E  +FPD+ A REIL L + CP K  GC   + L  +++H   C++                                    ++     K+  + S    +  C  CG +L   ++       L CP A + C F+  GC  K+ R  L  H++  T  H+ LL   +  +          + S        +++E I       + +D  +R   + + +    + +L R + +LE K                    +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L   +    Y+ +FVH M+G+ DS L WPF+G I LT + +    I +N  E +   PE   AF+RP   RNP GFG+  F+ + AL  G FI  ++TL+++ E+ T
Sbjct:   34 DSVSGSASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNK--GCLQKMELRHLEDHQVHCEFALVNCPQCQRPFQKCQVNTHIIEDCPRRQVSCVNCAVSMAYEEKEIHDQSCPLANIICEYCGTILIREQMPNHYD--LDCPTAPIPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLALRPCDAASPSRGCRPEDPNYEETIKQLESRLVRQDHQIRELTAKMETQSMYVGELKRTIRTLEDK-------------------VAEMEAQ--QCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEALIRQNHEEVMDAKPE-LLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIK-DDTLLVRCEVST 509          

BLAST of EMLSAG00000000227 vs. GO
Match: - (symbol:TRAF6 "TNF receptor-associated factor 6" species:9531 "Cercocebus atys" [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISS] [GO:0005811 "lipid particle" evidence=ISS] [GO:0043123 "positive regulation of I-kappaB kinase/NF-kappaB signaling" evidence=ISS] [GO:0051092 "positive regulation of NF-kappaB transcription factor activity" evidence=ISS] [GO:0070534 "protein K63-linked ubiquitination" evidence=ISS] InterPro:IPR001293 InterPro:IPR001841 InterPro:IPR002083 InterPro:IPR008974 InterPro:IPR012227 InterPro:IPR027139 Pfam:PF00917 PIRSF:PIRSF015614 PROSITE:PS50089 PROSITE:PS50144 PROSITE:PS50145 SMART:SM00061 SMART:SM00184 UniPathway:UPA00143 Prosite:PS00518 GO:GO:0005938 GO:GO:0005634 GO:GO:0050852 GO:GO:0005811 GO:GO:0046849 GO:GO:0043123 GO:GO:0008270 GO:GO:0009887 GO:GO:0004871 GO:GO:0042981 GO:GO:0051092 Gene3D:3.30.40.10 InterPro:IPR013083 GO:GO:0004842 GO:GO:0001503 GO:GO:0007250 InterPro:IPR017907 GO:GO:0070534 GO:GO:0048468 GO:GO:0051865 GO:GO:0045672 GO:GO:0043507 SUPFAM:SSF49599 GO:GO:0031666 GO:GO:0070498 HOVERGEN:HBG060248 PANTHER:PTHR10131:SF48 EMBL:EU204928 ProteinModelPortal:B6CJY4 Uniprot:B6CJY4)

HSP 1 Score: 211.46 bits (537), Expect = 2.579e-60
Identity = 148/496 (29.84%), Postives = 230/496 (46.37%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT------QERSSKKHEN-------PSRSSDSNTCPS-----------------------CGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSR-DSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSK---AFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D   G + T  +   + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CII  + + G  CP DN  L E  +FPD+ A REIL LM+ CP  + GC   + L  +++H   C++        Q    K H N       P R    + C +                       C  +L   ++       L CP A + C F++ GC  K+ R  L  H++  T  H+ +L   +  +    D+  ++   +  ET      EG L R D  +R   + + +    + +L R + +LE K                    +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L   +    Y+ +FVH M+G+ DS L WPF+G I LT + +      +  +EI +      AF+RP   RNP GFG+  F+ + AL    FI  ++TL+++ E+ T
Sbjct:   34 DSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCP--NEGCLHKMELRHLEDHQAHCEFALVDCPQCQRPFQKFHINIHILKDCPRRQVSCDNCAALVAFEDKEIHDQNCPLANVICEYCNTILIREQMPNHYD--LDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLAQAVHSLSLIPDSGYVSEVRNFQETIHQ--LEGRLVRQDHQIRELTAKMETQSTYVSELKRTIRTLEDK-------------------VAEIEAQ--QCNGIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPDLLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIK-DDTLLVRCEVST 501          

BLAST of EMLSAG00000000227 vs. GO
Match: - (symbol:TRAF6 "TNF receptor-associated factor 6" species:9544 "Macaca mulatta" [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISS] [GO:0005811 "lipid particle" evidence=ISS] [GO:0043123 "positive regulation of I-kappaB kinase/NF-kappaB signaling" evidence=ISS] [GO:0051092 "positive regulation of NF-kappaB transcription factor activity" evidence=ISS] [GO:0070534 "protein K63-linked ubiquitination" evidence=ISS] InterPro:IPR001293 InterPro:IPR001841 InterPro:IPR002083 InterPro:IPR008974 InterPro:IPR012227 InterPro:IPR027139 Pfam:PF00917 PIRSF:PIRSF015614 PROSITE:PS50089 PROSITE:PS50144 PROSITE:PS50145 SMART:SM00061 SMART:SM00184 UniPathway:UPA00143 Prosite:PS00518 GO:GO:0005938 GO:GO:0005634 GO:GO:0050852 GO:GO:0005811 GO:GO:0046849 GO:GO:0043123 GO:GO:0008270 GO:GO:0009887 GO:GO:0004871 GO:GO:0042981 GO:GO:0051092 Gene3D:3.30.40.10 InterPro:IPR013083 GO:GO:0004842 GO:GO:0001503 GO:GO:0007250 InterPro:IPR017907 GO:GO:0070534 GO:GO:0048468 GO:GO:0051865 GO:GO:0045672 GO:GO:0043507 SUPFAM:SSF49599 GO:GO:0031666 GO:GO:0070498 CTD:7189 eggNOG:NOG289765 HOGENOM:HOG000006625 HOVERGEN:HBG060248 KO:K03175 PANTHER:PTHR10131:SF48 EMBL:EU204929 RefSeq:NP_001129268.1 UniGene:Mmu.17706 STRING:9544.ENSMMUP00000021907 GeneID:716907 KEGG:mcc:716907 NextBio:19981456 ArrayExpress:B6CJY5 Uniprot:B6CJY5)

HSP 1 Score: 211.46 bits (537), Expect = 3.137e-60
Identity = 148/496 (29.84%), Postives = 230/496 (46.37%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQG--GYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT------QERSSKKHEN-------PSRSSDSNTCPS-----------------------CGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSR-DSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSK---AFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D   G + T  +   + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CII  + + G  CP DN  L E  +FPD+ A REIL LM+ CP  + GC   + L  +++H   C++        Q    K H N       P R    + C +                       C  +L   ++       L CP A + C F++ GC  K+ R  L  H++  T  H+ +L   +  +    D+  ++   +  ET      EG L R D  +R   + + +    + +L R + +LE K                    +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L   +    Y+ +FVH M+G+ DS L WPF+G I LT + +      +  +EI +      AF+RP   RNP GFG+  F+ + AL    FI  ++TL+++ E+ T
Sbjct:   34 DSVGGTASTGNLSSSFMEDIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCP--NEGCLHKMELRHLEDHQAHCEFALVDCPQCQRPFQKFHINIHILKDCPRRQVSCDNCAALVAFEDKEIHDQNCPLANVICEYCNTILIREQMPNHYD--LDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLAQAVHSLSLIPDSGYVSEVRNFQETIHQ--LEGRLVRQDHQIRELTAKMETQSTYVSELKRTIRTLEDK-------------------VAEIEAQ--QCNGIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPDLLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIK-DDTLLVRCEVST 501          

BLAST of EMLSAG00000000227 vs. GO
Match: - (symbol:Traf6 "TNF receptor-associated factor 6, E3 ubiquitin protein ligase" species:10116 "Rattus norvegicus" [GO:0000122 "negative regulation of transcription from RNA polymerase II promoter" evidence=IEA;ISO] [GO:0000209 "protein polyubiquitination" evidence=ISO] [GO:0001503 "ossification" evidence=IEA;ISO] [GO:0001843 "neural tube closure" evidence=IEA;ISO] [GO:0002726 "positive regulation of T cell cytokine production" evidence=IEA;ISO] [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISO;ISS] [GO:0005622 "intracellular" evidence=ISO] [GO:0005634 "nucleus" evidence=ISO] [GO:0005730 "nucleolus" evidence=ISO] [GO:0005737 "cytoplasm" evidence=ISO] [GO:0005739 "mitochondrion" evidence=ISO] [GO:0005811 "lipid particle" evidence=ISO;ISS] [GO:0005829 "cytosol" evidence=IEA;ISO] [GO:0005886 "plasma membrane" evidence=ISO] [GO:0005938 "cell cortex" evidence=IEA] [GO:0006461 "protein complex assembly" evidence=IMP] [GO:0006955 "immune response" evidence=ISO] [GO:0007165 "signal transduction" evidence=ISO] [GO:0007249 "I-kappaB kinase/NF-kappaB signaling" evidence=IEA;ISO] [GO:0007250 "activation of NF-kappaB-inducing kinase activity" evidence=IEA;ISO] [GO:0007254 "JNK cascade" evidence=IMP] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0009887 "organ morphogenesis" evidence=ISO] [GO:0009898 "cytoplasmic side of plasma membrane" evidence=IEA;ISO] [GO:0016567 "protein ubiquitination" evidence=ISO] [GO:0019221 "cytokine-mediated signaling pathway" evidence=IMP] [GO:0019886 "antigen processing and presentation of exogenous peptide antigen via MHC class II" evidence=IEA;ISO] [GO:0019901 "protein kinase binding" evidence=ISO] [GO:0030316 "osteoclast differentiation" evidence=IEA;ISO] [GO:0031435 "mitogen-activated protein kinase kinase kinase binding" evidence=IEA;ISO] [GO:0031625 "ubiquitin protein ligase binding" evidence=IEA;ISO] [GO:0031666 "positive regulation of lipopolysaccharide-mediated signaling pathway" evidence=IEA;ISO] [GO:0031965 "nuclear membrane" evidence=IEA] [GO:0031996 "thioesterase binding" evidence=IEA;ISO] [GO:0032147 "activation of protein kinase activity" evidence=ISO] [GO:0032743 "positive regulation of interleukin-2 production" evidence=IEA;ISO] [GO:0035631 "CD40 receptor complex" evidence=IEA;ISO] [GO:0042088 "T-helper 1 type immune response" evidence=IEA;ISO] [GO:0042102 "positive regulation of T cell proliferation" evidence=IEA;ISO] [GO:0042475 "odontogenesis of dentin-containing tooth" evidence=IEA;ISO] [GO:0042826 "histone deacetylase binding" evidence=IEA;ISO] [GO:0042981 "regulation of apoptotic process" evidence=IEA] [GO:0043011 "myeloid dendritic cell differentiation" evidence=IEA;ISO] [GO:0043123 "positive regulation of I-kappaB kinase/NF-kappaB signaling" evidence=ISO;ISS] [GO:0043234 "protein complex" evidence=IMP] [GO:0043422 "protein kinase B binding" evidence=IEA;ISO] [GO:0043507 "positive regulation of JUN kinase activity" evidence=IEA;ISO] [GO:0045084 "positive regulation of interleukin-12 biosynthetic process" evidence=IEA;ISO] [GO:0045410 "positive regulation of interleukin-6 biosynthetic process" evidence=IEA;ISO] [GO:0045453 "bone resorption" evidence=IEA;ISO] [GO:0045672 "positive regulation of osteoclast differentiation" evidence=IEA;ISO] [GO:0045892 "negative regulation of transcription, DNA-templated" evidence=ISO] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IEA;ISO] [GO:0046849 "bone remodeling" evidence=ISO] [GO:0047485 "protein N-terminus binding" evidence=IEA;ISO] [GO:0048468 "cell development" evidence=IEA;ISO] [GO:0048471 "perinuclear region of cytoplasm" evidence=IEA;ISO] [GO:0048661 "positive regulation of smooth muscle cell proliferation" evidence=IMP] [GO:0050852 "T cell receptor signaling pathway" evidence=IEA;ISO] [GO:0051023 "regulation of immunoglobulin secretion" evidence=IEA;ISO] [GO:0051091 "positive regulation of sequence-specific DNA binding transcription factor activity" evidence=ISO] [GO:0051092 "positive regulation of NF-kappaB transcription factor activity" evidence=ISO;ISS] [GO:0051865 "protein autoubiquitination" evidence=ISO] [GO:0070498 "interleukin-1-mediated signaling pathway" evidence=ISO;IMP] [GO:0070534 "protein K63-linked ubiquitination" evidence=ISO;ISS] [GO:0070555 "response to interleukin-1" evidence=ISO] [GO:2000679 "positive regulation of transcription regulatory region DNA binding" evidence=IEA;ISO] InterPro:IPR001293 InterPro:IPR001841 InterPro:IPR002083 InterPro:IPR008974 InterPro:IPR012227 InterPro:IPR027139 Pfam:PF00917 PIRSF:PIRSF015614 PROSITE:PS50089 PROSITE:PS50144 PROSITE:PS50145 SMART:SM00061 SMART:SM00184 UniPathway:UPA00143 RGD:1306853 Prosite:PS00518 GO:GO:0005829 GO:GO:0005938 GO:GO:0048471 GO:GO:0043234 GO:GO:0006461 GO:GO:0019886 GO:GO:0050852 GO:GO:0042088 GO:GO:0007254 GO:GO:0005811 GO:GO:0043123 GO:GO:0008270 GO:GO:0031965 GO:GO:0048661 GO:GO:0045944 GO:GO:0000122 GO:GO:0004871 GO:GO:0042981 GO:GO:0051092 Gene3D:3.30.40.10 InterPro:IPR013083 GO:GO:0042102 GO:GO:0001843 GO:GO:0042475 GO:GO:0045453 GO:GO:0004842 GO:GO:0001503 GO:GO:0007250 GO:GO:0009898 InterPro:IPR017907 GO:GO:0070534 GO:GO:0048468 GO:GO:0051865 GO:GO:0045672 GO:GO:0043507 GO:GO:0007249 SUPFAM:SSF49599 GO:GO:0043011 GO:GO:0035631 EMBL:CH473949 GO:GO:0030316 GO:GO:0032743 GO:GO:0031666 GO:GO:0051023 GO:GO:0070498 GO:GO:0045410 GO:GO:2000679 GO:GO:0045084 GO:GO:0002726 OrthoDB:EOG7966G5 TreeFam:TF321154 GeneTree:ENSGT00550000074359 CTD:7189 eggNOG:NOG289765 HOGENOM:HOG000006625 HOVERGEN:HBG060248 KO:K03175 OMA:NFQETIH PANTHER:PTHR10131:SF48 EMBL:BC168921 RefSeq:NP_001101224.1 UniGene:Rn.220435 UniGene:Rn.24348 ProteinModelPortal:B5DF45 BioGrid:259875 STRING:10116.ENSRNOP00000006148 PhosphoSite:B5DF45 PRIDE:B5DF45 Ensembl:ENSRNOT00000006148 GeneID:311245 KEGG:rno:311245 UCSC:RGD:1306853 NextBio:663249 PRO:PR:B5DF45 Genevestigator:B5DF45 Uniprot:B5DF45)

HSP 1 Score: 210.69 bits (535), Expect = 6.313e-60
Identity = 147/482 (30.50%), Postives = 225/482 (46.68%), Query Frame = 0

Query:   21 GYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT----------QERSSKKH---ENPSRSSD----------------SNTCP-------SCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGI-------LSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVRE----ISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CI   + + G  CP DN  L E  +FPD+ A REIL L + CP K  GC   + L  +++H   C++            Q+    KH   + P R                     +CP        CG +L   ++       L CP A V C F+  GC  K+ R  L  H++  T  H+ LL   +  +  +      ++ S        +++E +       + +D  +R   + + +    + +L R + SLE K                    +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L   +    Y+ +FVH M+G+ DS L WPF+G I LT + +    I +N  E +   PE   AF+RP   RNP GFG+  F+ + AL  G FI  ++TL+++ E+ T
Sbjct:   55 GYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACITKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNK--GCVQKMELRHLEDHQVHCEFALVICPQCQRFFQKCQINKHIIEDCPRRQVSCVNCAVPMPYEEKEIHDQSCPLANIICEYCGTILIREQMPNHYD--LDCPTAPVPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLSLRPCDASSPSRGCRPEDPNYEETVKQLEGRLVRQDHQIRELTAKMETQSMHVSELKRTIRSLEDK-------------------VAEMEAQ--QCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAVIRQNHEEVMDAKPE-LLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIK-DDTLLVRCEVST 509          

BLAST of EMLSAG00000000227 vs. GO
Match: - (symbol:TRAF6 "TNF receptor-associated factor 6" species:9823 "Sus scrofa" [GO:0000122 "negative regulation of transcription from RNA polymerase II promoter" evidence=IEA] [GO:0001503 "ossification" evidence=IEA] [GO:0001843 "neural tube closure" evidence=IEA] [GO:0002726 "positive regulation of T cell cytokine production" evidence=IEA] [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISS] [GO:0005811 "lipid particle" evidence=ISS] [GO:0005829 "cytosol" evidence=IEA] [GO:0005938 "cell cortex" evidence=IEA] [GO:0007249 "I-kappaB kinase/NF-kappaB signaling" evidence=IEA] [GO:0007250 "activation of NF-kappaB-inducing kinase activity" evidence=IEA] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0009898 "cytoplasmic side of plasma membrane" evidence=IEA] [GO:0019886 "antigen processing and presentation of exogenous peptide antigen via MHC class II" evidence=IEA] [GO:0030316 "osteoclast differentiation" evidence=IEA] [GO:0031435 "mitogen-activated protein kinase kinase kinase binding" evidence=IEA] [GO:0031625 "ubiquitin protein ligase binding" evidence=IEA] [GO:0031666 "positive regulation of lipopolysaccharide-mediated signaling pathway" evidence=IEA] [GO:0031965 "nuclear membrane" evidence=IEA] [GO:0031996 "thioesterase binding" evidence=IEA] [GO:0032743 "positive regulation of interleukin-2 production" evidence=IEA] [GO:0035631 "CD40 receptor complex" evidence=IEA] [GO:0042088 "T-helper 1 type immune response" evidence=IEA] [GO:0042102 "positive regulation of T cell proliferation" evidence=IEA] [GO:0042475 "odontogenesis of dentin-containing tooth" evidence=IEA] [GO:0042826 "histone deacetylase binding" evidence=IEA] [GO:0042981 "regulation of apoptotic process" evidence=IEA] [GO:0043011 "myeloid dendritic cell differentiation" evidence=IEA] [GO:0043123 "positive regulation of I-kappaB kinase/NF-kappaB signaling" evidence=ISS] [GO:0043422 "protein kinase B binding" evidence=IEA] [GO:0043507 "positive regulation of JUN kinase activity" evidence=IEA] [GO:0045084 "positive regulation of interleukin-12 biosynthetic process" evidence=IEA] [GO:0045410 "positive regulation of interleukin-6 biosynthetic process" evidence=IEA] [GO:0045453 "bone resorption" evidence=IEA] [GO:0045672 "positive regulation of osteoclast differentiation" evidence=IEA] [GO:0045944 "positive regulation of transcription from RNA polymerase II promoter" evidence=IEA] [GO:0047485 "protein N-terminus binding" evidence=IEA] [GO:0048468 "cell development" evidence=IEA] [GO:0048471 "perinuclear region of cytoplasm" evidence=IEA] [GO:0050852 "T cell receptor signaling pathway" evidence=IEA] [GO:0051023 "regulation of immunoglobulin secretion" evidence=IEA] [GO:0051092 "positive regulation of NF-kappaB transcription factor activity" evidence=ISS] [GO:0070498 "interleukin-1-mediated signaling pathway" evidence=IEA] [GO:0070534 "protein K63-linked ubiquitination" evidence=ISS] [GO:2000679 "positive regulation of transcription regulatory region DNA binding" evidence=IEA] InterPro:IPR001293 InterPro:IPR001841 InterPro:IPR002083 InterPro:IPR008974 InterPro:IPR012227 InterPro:IPR027139 Pfam:PF00917 PIRSF:PIRSF015614 PROSITE:PS50089 PROSITE:PS50144 PROSITE:PS50145 SMART:SM00061 SMART:SM00184 UniPathway:UPA00143 Prosite:PS00518 GO:GO:0005829 GO:GO:0005938 GO:GO:0048471 GO:GO:0019886 GO:GO:0050852 GO:GO:0042088 GO:GO:0005811 GO:GO:0043123 GO:GO:0008270 GO:GO:0031965 GO:GO:0045944 GO:GO:0000122 GO:GO:0004871 GO:GO:0042981 GO:GO:0051092 Gene3D:3.30.40.10 InterPro:IPR013083 GO:GO:0042102 GO:GO:0001843 GO:GO:0042475 GO:GO:0045453 GO:GO:0004842 GO:GO:0001503 GO:GO:0007250 GO:GO:0009898 InterPro:IPR017907 GO:GO:0070534 GO:GO:0048468 GO:GO:0051865 GO:GO:0045672 GO:GO:0043507 GO:GO:0007249 SUPFAM:SSF49599 GO:GO:0043011 GO:GO:0035631 GO:GO:0030316 GO:GO:0032743 GO:GO:0031666 GO:GO:0051023 GO:GO:0070498 GO:GO:0045410 GO:GO:2000679 GO:GO:0045084 GO:GO:0002726 OrthoDB:EOG7966G5 TreeFam:TF321154 GeneTree:ENSGT00550000074359 CTD:7189 eggNOG:NOG289765 HOGENOM:HOG000006625 HOVERGEN:HBG060248 KO:K03175 OMA:NFQETIH PANTHER:PTHR10131:SF48 EMBL:EU095967 RefSeq:NP_001098756.1 RefSeq:XP_005652858.1 UniGene:Ssc.96412 ProteinModelPortal:A7XUJ6 SMR:A7XUJ6 STRING:9823.ENSSSCP00000014123 Ensembl:ENSSSCT00000014516 Ensembl:ENSSSCT00000033074 GeneID:396629 KEGG:ssc:396629 Uniprot:A7XUJ6)

HSP 1 Score: 210.305 bits (534), Expect = 9.052e-60
Identity = 157/503 (31.21%), Postives = 241/503 (47.91%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQ-----ERSSKK--------HENPSRS-----------------SDSN------TCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHST-QKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVM------CSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVRE----ISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D  +G + T  +   + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CII  + + G  CP DN  L E  +FPD+ A REIL L + CP  + GC   + L  +++H   C++        +R  +K         E P R                   D N       C  C  VL   ++       L CP A V C F++ GC  K+ R  L  H++  T  H+ ++   L+ +       SL   + V +     +    LSR S    S  N   T Q+L   L R+   + +   ++E ++ M   +LK+ +       +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L   +    Y+ +FVH M+G+ DS L WPF+G I LT + +    I +N  E +   PE   AF+RP   RNP GFG+  F+ ++AL    FI  ++TL+++ E+ T
Sbjct:   35 DGVSGTASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCP--NVGCLHKMELRHLEDHQAHCEFALMNCPQCQRPFQKCQLNIHILKECPRRQVSCVNCAVSMAFEDKEIHDQNCPLANVICEYCNTVLIREQMPNHYD--LDCPTAPVPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMMAQALQGL-------SLAV-APVPQRDMLPYDSSPLSRISSGCCSDQNFQETIQQLEGRLVRQDHQIRELTAKMETQS-MYVSELKRTIRSLEDKVAEIEAQ--QCNGIYIWKIGNFGMHLKSQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPIRQNHEEIMDAKPE-LLAFQRPTIPRNPKGFGYVTFMHLDALRQRTFIK-DDTLLVRCEVST 520          

BLAST of EMLSAG00000000227 vs. GO
Match: - (symbol:traf6-b "TNF receptor-associated factor 6-B" species:8355 "Xenopus laevis" [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISS] [GO:0005515 "protein binding" evidence=IPI] [GO:0007250 "activation of NF-kappaB-inducing kinase activity" evidence=IDA] [GO:0070534 "protein K63-linked ubiquitination" evidence=ISS] InterPro:IPR001293 InterPro:IPR001841 InterPro:IPR002083 InterPro:IPR008974 InterPro:IPR012227 InterPro:IPR027139 Pfam:PF00917 PIRSF:PIRSF015614 PROSITE:PS50089 PROSITE:PS50144 PROSITE:PS50145 SMART:SM00061 SMART:SM00184 UniPathway:UPA00143 Prosite:PS00518 GO:GO:0005737 GO:GO:0050852 GO:GO:0046849 GO:GO:0043123 GO:GO:0008270 GO:GO:0009887 GO:GO:0004871 GO:GO:0042981 Gene3D:3.30.40.10 InterPro:IPR013083 GO:GO:0004842 GO:GO:0001503 GO:GO:0007250 InterPro:IPR017907 GO:GO:0070534 GO:GO:0048468 GO:GO:0051865 GO:GO:0045672 GO:GO:0043507 SUPFAM:SSF49599 GO:GO:0031666 GO:GO:0070498 HOVERGEN:HBG060248 KO:K03175 PANTHER:PTHR10131:SF48 EMBL:BC075143 RefSeq:NP_001086348.1 UniGene:Xl.29694 ProteinModelPortal:Q6DJN2 SMR:Q6DJN2 GeneID:444777 KEGG:xla:444777 CTD:444777 Xenbase:XB-GENE-865394 Uniprot:Q6DJN2)

HSP 1 Score: 209.534 bits (532), Expect = 2.956e-59
Identity = 137/483 (28.36%), Postives = 225/483 (46.58%), Query Frame = 0

Query:   21 GYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNT----------CPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSA---------------------------GCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILS----------RDSDLRLSGSNLHST-QKLIKDLFRRVVSLEQKNCQLEIR----NEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSK---AFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLI 444
            GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CI+  L + G  CP DN  L E  +FPD+ A REIL L + CP +  GC   + L  ++ HL  C++ + E S  +   P    + +           C +CG  +   +++   G+ L+CP A V C +                              GC  K+ R +L  H++  T  H+ +++  LR    ++ T   +  S +     + F+   LS             D      NL  T ++L   L R+   + +   ++E +    NE L+  ++ +     +  G +CNG F+WK+  F  L K    +   V++S GFYT   GYK+CLR ++ L + +    Y+ +FVH M+G+ DS L WP  G I L+ + +      +  +E+ +      AF+RP ++RNP GFG+  F+ +N L    ++  +   +
Sbjct:   57 GYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIVKSLRDAGHKCPVDNEILMENQLFPDNFAKREILSLKVKCPSQ--GCTETMELRHLERHLGQCQFASVECSQCQGSFPKSRLEKHMEHECGRRKIFCDNCGLAMAYEDMS---GHELICPLAYVTCEYCQTNLIREQMPSHYSMDCTMAPIPCMYYEFGCTEKMQRNDLARHLQDFTQAHMRMMFQTLR----SFSTTPTSHISDISFCDPNQFEPVPLSVAPAHPSHMPSHQDCSQETRNLRETIEQLEGRLVRQDHQIRELIAKMETQCTYVNE-LKHTIRSLEDKLLDVDGHHCNGVFIWKIKGFSGLQKTQEEEKPVVIHSPGFYTGKPGYKLCLRLHLQLPSAQRCANYISLFVHTMQGEYDSLLPWPLHGTIRLSILDQSEGAIIQDQEEVMDTKPDLLAFQRPTTQRNPKGFGYVTFMHLNTLKQRQYVKNDTLFV 529          

BLAST of EMLSAG00000000227 vs. GO
Match: - (symbol:traf6 "TNF receptor-associated factor 6" species:7955 "Danio rerio" [GO:0000209 "protein polyubiquitination" evidence=IEA] [GO:0043507 "positive regulation of JUN kinase activity" evidence=IEA] [GO:0048468 "cell development" evidence=IEA] [GO:0070498 "interleukin-1-mediated signaling pathway" evidence=IEA] [GO:0004871 "signal transducer activity" evidence=IEA] [GO:0008270 "zinc ion binding" evidence=IEA] [GO:0016567 "protein ubiquitination" evidence=IEA] [GO:0042981 "regulation of apoptotic process" evidence=IEA] [GO:0045672 "positive regulation of osteoclast differentiation" evidence=IEA] [GO:0050852 "T cell receptor signaling pathway" evidence=IEA] [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA;ISS] [GO:0007165 "signal transduction" evidence=IEA] [GO:0001503 "ossification" evidence=IEA] [GO:0031666 "positive regulation of lipopolysaccharide-mediated signaling pathway" evidence=IEA] [GO:0043123 "positive regulation of I-kappaB kinase/NF-kappaB signaling" evidence=IEA] [GO:0046849 "bone remodeling" evidence=IEA] [GO:0051865 "protein autoubiquitination" evidence=IEA] [GO:0007250 "activation of NF-kappaB-inducing kinase activity" evidence=IEA] [GO:0009887 "organ morphogenesis" evidence=IEA] [GO:0070534 "protein K63-linked ubiquitination" evidence=ISS] [GO:0016874 "ligase activity" evidence=IEA] [GO:0046872 "metal ion binding" evidence=IEA] [GO:0005737 "cytoplasm" evidence=IEA] [GO:0009617 "response to bacterium" evidence=IDA] [GO:0045893 "positive regulation of transcription, DNA-templated" evidence=IDA] [GO:0009615 "response to virus" evidence=IDA] [GO:0045087 "innate immune response" evidence=IMP] InterPro:IPR001293 InterPro:IPR001841 InterPro:IPR002083 InterPro:IPR008974 InterPro:IPR012227 InterPro:IPR013323 InterPro:IPR027139 Pfam:PF00917 PIRSF:PIRSF015614 PROSITE:PS50089 PROSITE:PS50144 PROSITE:PS50145 SMART:SM00061 SMART:SM00184 UniPathway:UPA00143 Prosite:PS00518 ZFIN:ZDB-GENE-030131-5735 GO:GO:0005737 GO:GO:0045087 GO:GO:0045893 GO:GO:0009617 GO:GO:0050852 GO:GO:0046849 GO:GO:0043123 GO:GO:0008270 GO:GO:0009615 GO:GO:0009887 GO:GO:0004871 GO:GO:0042981 Gene3D:3.30.40.10 InterPro:IPR013083 GO:GO:0004842 GO:GO:0001503 GO:GO:0007250 InterPro:IPR017907 GO:GO:0070534 GO:GO:0048468 GO:GO:0051865 GO:GO:0045672 GO:GO:0043507 SUPFAM:SSF49599 GO:GO:0031666 GO:GO:0070498 Gene3D:3.90.890.10 OrthoDB:EOG7966G5 TreeFam:TF321154 GeneTree:ENSGT00550000074359 CTD:7189 eggNOG:NOG289765 HOGENOM:HOG000006625 HOVERGEN:HBG060248 KO:K03175 PANTHER:PTHR10131:SF48 EMBL:AY616583 EMBL:AL929208 EMBL:BC055214 EMBL:BC171483 EMBL:BC171487 RefSeq:NP_001038217.1 UniGene:Dr.74618 ProteinModelPortal:Q6IWL4 SMR:Q6IWL4 STRING:7955.ENSDARP00000036715 Ensembl:ENSDART00000038273 GeneID:554561 KEGG:dre:554561 InParanoid:Q7SXX1 OMA:IECEDES NextBio:20880774 PRO:PR:Q6IWL4 Bgee:Q6IWL4 Uniprot:Q6IWL4)

HSP 1 Score: 207.994 bits (528), Expect = 7.865e-59
Identity = 148/478 (30.96%), Postives = 225/478 (47.07%), Query Frame = 0

Query:   19 QGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT------QERSSKKHENPSRSSDS----NTCPSCGN-----VLDDHEITQGQGNRLVCPY--------------------AIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLR-KIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEM-------LQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNG---EEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAEL 449
            Q GYD +F   L  ++ECPICL+  R   QT CGHRFC SCI   + + G+ CP DN  L E  +FPD+ A REIL L + C   + GC   + L  ++ HL  C++ T      QE     H +  +S        TCP C       V  +HE      N  VC Y                    A VAC F++ GC  K+ R EL  H++  T  H+  + + LR +   N   PS     S D+  +S+       R  D       L + ++ + +L  R+V  +Q+  +L I N+        L+ KL  +  S  E + +   G +VW++ +F    +        V++S  FYT   GYK+CLR ++   +      ++ +FVH M+G+ DS LSWP +G I L  + ++       + E     +AF+RP   RNP GFG+  F+ + AL   GF+  E+ L+++ E+
Sbjct:   54 QQGYDVEFDPPLESKYECPICLMGLRSAVQTPCGHRFCDSCIRKSIRDTGQKCPVDNEVLLEEQLFPDNFAKREILSLTVKC--SNFGCSEKMELRQLEKHLSQCRFATAPCPQCQESVPISHLDEHKSQHCLQRIMTCPDCAGSFVYAVKQNHEQFCPFAN-TVCEYCEMELIRDQLALHCDTDCLKAPVACTFSTFGCREKMTRNELAQHMQEFTQMHMRYMAEFLRSQTLNNCTMPSAAAHLSSDDRGASA-------RSPDSCQCKQELLNLRETVLELEGRLVRQDQQIRELCIHNDTQKNQVTELRRKLVSLEESTRELEAQQYQGIYVWRVENFSHHLRNQEAGQPIVLHSPPFYTGRPGYKLCLRLHLQTPSAPRCSNFISLFVHTMQGEFDSQLSWPLQGTIRLAVLDQVEGQHHIEVMETKPDLQAFQRPTVMRNPKGFGYVTFLHLQALRQRGFVK-EDVLLVRCEV 520          

BLAST of EMLSAG00000000227 vs. C. finmarchicus
Match: gi|592936045|gb|GAXK01022508.1| (TSA: Calanus finmarchicus comp797198_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 459.144 bits (1180), Expect = 9.595e-157
Identity = 238/464 (51.29%), Postives = 316/464 (68.10%), Query Frame = 0

Query:    1 MDKETGRSGTKGIPKHYFQGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGIXXX--------XXXXXXXGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQ-GRYCNGKFVWKLLDFPELHKKMRTDCH-FVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVRE----ISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTEN-TLILKAEL 449
            M    GR  T  +  HY+QGGYD+ FV  +   +ECPICLLCQRDPHQTSCGHRFCYSCI+TWL+EG+TCP DN S+GEGDIFPD++A+REI+QL + CP  D GC   I +++ ++HL SCK+ +  +++K        S+S TC SCG+++ D  + + QG  L+CP  +VAC FTS GC  KI R+ LQ H+ S+T  H+ LL +KL K+QQ      + T S  D+          L             LR+SGSN+HS  KLI++L+++VVSLEQK  Q EI+ E L+++L +    + + Q GRYCNG FVWK+ +F E+H+KMR +CH FVVYSKGFYTS FGYK+CLRSN+    G+E+LGVF+HLMRG+ND  L+WP+ G I+LT   +    I E+F ETI  +P G  AF+RP  ERN  GFGF EFIRVN+LY+GGF+  EN TLI+KA++
Sbjct:   61 MSSREGRIKTTAV--HYYQGGYDFQFVSGMDPVYECPICLLCQRDPHQTSCGHRFCYSCIMTWLSEGKTCPHDNTSIGEGDIFPDTIANREIMQLNVLCPNSDQGCPLSISIAECESHLSSCKFGSSPQTNK--------SESVTCTSCGDLVQDQTMAKHQG--LICPNKLVACSFTSIGCTQKIPRKHLQHHLASETGQHMQLLAEKLAKVQQLQQAEQVLTNSYPDQYQDEDQPSSSLPSSPRLNRGHQDVLRMSGSNMHSQSKLIRELYQKVVSLEQKGYQQEIKIEQLEQQLARTQLDQDDDQAGRYCNGSFVWKIRNFSEIHQKMR-NCHSFVVYSKGFYTSAFGYKMCLRSNVYYTEGDEHLGVFLHLMRGENDDCLTWPWGGSITLTIFNQREGIIREHFSETIDSMP-GLAAFDRPSEERNTRGFGFQEFIRVNSLYTGGFLLAENDTLIIKADV 1410          

BLAST of EMLSAG00000000227 vs. C. finmarchicus
Match: gi|592936044|gb|GAXK01022509.1| (TSA: Calanus finmarchicus comp797198_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 218.009 bits (554), Expect = 7.262e-67
Identity = 104/198 (52.53%), Postives = 135/198 (68.18%), Query Frame = 0

Query:    1 MDKETGRSGTKGIPKHYFQGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQ 198
            M    GR  T  +  HY+QGGYD+ FV  +   +ECPICLLCQRDPHQTSCGHRFCYSCI+TWL+EG+TCP DN S+GEGDIFPD++A+REI+QL + CP  DHGC   I L+D ++HL SCK+    ++ +         +S TC SCG+++ D   T  +   L+CP  +VAC FTS GC  KI R+ LQ H+ S+
Sbjct:   61 MSSREGRIKTTAV--HYYQGGYDFQFVSGMDPVYECPICLLCQRDPHQTSCGHRFCYSCIMTWLSEGKTCPHDNTSIGEGDIFPDTIANREIMQLNVLCPNSDHGCPVSIRLADCESHLSSCKFRPLPQTQQL--------ESVTCTSCGDLIQDQ--TMAKHLSLICPNKLVACSFTSIGCIQKIPRKHLQHHLASE 618          

BLAST of EMLSAG00000000227 vs. C. finmarchicus
Match: gi|592936043|gb|GAXK01022510.1| (TSA: Calanus finmarchicus comp797198_c0_seq3 transcribed RNA sequence)

HSP 1 Score: 162.54 bits (410), Expect = 1.085e-46
Identity = 70/114 (61.40%), Postives = 88/114 (77.19%), Query Frame = 0

Query:    1 MDKETGRSGTKGIPKHYFQGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSD 114
            M    GR  T  +  HY+QGGYD+ FV  +   +ECPICLLCQRDPHQTSCGHRFCYSCI+TWL+EG+TCP DN S+GEGDIFPD++A+REI+QL ++CP  D GC   I +++
Sbjct:   61 MSSREGRIKTTAV--HYYQGGYDFQFVSGMDPVYECPICLLCQRDPHQTSCGHRFCYSCIMTWLSEGKTCPHDNTSIGEGDIFPDTIANREIMQLSVSCPNSDQGCPVSIRITE 396          

BLAST of EMLSAG00000000227 vs. C. finmarchicus
Match: gi|592759068|gb|GAXK01195345.1| (TSA: Calanus finmarchicus comp452427_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 64.3142 bits (155), Expect = 2.462e-10
Identity = 46/180 (25.56%), Postives = 89/180 (49.44%), Query Frame = 0

Query:  277 QLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE--YLGVFVHLMRGDNDSALSWPFRGQISLTAV-----REISENFFETIKEIPEGS-KAFERPIS--ERNPTGFGFTEFIRVNALYSGGFINTENTLILK 446
            Q+ + +   Q ++   + ++ E      +G  VW++ +F +   + R      + S+ F+TS  GYK+     +N   G E  ++ +++ ++ G+ D  L WPF+  IS T +     R  + N  E+   IP+ +   F RP +  +++  GFGF +F+  + L   G++  E TL +K
Sbjct:   91 QMMVAHTKRQAEIIAALQTKIEEASTSKDGTLVWRISNFSQKMMESRKSEGLELVSRPFFTSSTGYKLQASLFLNGNGGGENTHMSLYIKVLPGEYDCILKWPFKHTISFTLLDQNMDRTAAVNVMESF--IPDQNWPNFNRPSTCNDQDQLGFGFPKFVHQDILTKRGYVKDE-TLFIK 621          

BLAST of EMLSAG00000000227 vs. C. finmarchicus
Match: gi|592764614|gb|GAXK01189954.1| (TSA: Calanus finmarchicus comp263537_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 61.6178 bits (148), Expect = 2.613e-9
Identity = 45/159 (28.30%), Postives = 70/159 (44.03%), Query Frame = 0

Query:  295 SEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE--YLGVFVHLMRGDNDSALSWPFRGQISLTAV-----REISENFFETIKEIPEGSKAFERPISERNP--TGFGFTEFIRVNALYSGGFINTENTLI 444
            +E E      +G  +WK+    E  ++ R      + S  F+TS  GYK+     +    G E  +L +++ ++ GD DS L WPFR  IS T +     R  + N  E+    P     F+RP    +P   GFGF +F+    L    +I  +   I
Sbjct:  963 AELEKACTSKDGVLIWKIKGVAEKMQEARGSDGLELVSLPFFTSDCGYKLQASLFLGGNGGGEDTHLSIYIKVLPGDYDSILKWPFRHTISFTLLDQNPDRNSAVNIVESFIPDPNWPN-FQRPSQYEDPDQLGFGFPKFVPHGMLNIRNYIKDDTMFI 1436          

BLAST of EMLSAG00000000227 vs. C. finmarchicus
Match: gi|592792492|gb|GAXK01162076.1| (TSA: Calanus finmarchicus comp34828_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 60.4622 bits (145), Expect = 4.293e-9
Identity = 35/109 (32.11%), Postives = 52/109 (47.71%), Query Frame = 0

Query:   18 FQGGYDYDFVQQLPKRFECPICLLCQRDPH-QTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREIL-QLMITCPQKDHGCGAVIHLSDVZNHLRSCKY 124
            F+G  D + +        CPIC     DP     C H FC  CI  WLN  +TCP D  S+   ++ P     + +L +L I C  + +GC  V+ L  +  HL+ C++
Sbjct:   65 FEGSVDEELI--------CPICSAVLEDPRLAPECEHAFCADCIHEWLNRQQTCPVDRRSITVPELKPVPRILKNLLSRLEIRCDNELYGCTVVVKLDILAMHLKECEH 367          

BLAST of EMLSAG00000000227 vs. C. finmarchicus
Match: gi|592851296|gb|GAXK01106248.1| (TSA: Calanus finmarchicus comp118993_c1_seq1 transcribed RNA sequence)

HSP 1 Score: 53.5286 bits (127), Expect = 1.077e-6
Identity = 46/188 (24.47%), Postives = 74/188 (39.36%), Query Frame = 0

Query:   34 FECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGD-IFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQ--GNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYD 218
            + CPIC     + H T CGH FCY C++  + +   CP  N +L   D IFP+ + +  + +                + + ++N LRS   +     +      S +SDS + P    +L   E  + Q   N L+               A K + RE   H+K Q    +  L  +   IQ + D
Sbjct: 1499 YLCPICFELITEAHMTRCGHTFCYPCLLQTIEQSARCPKCNFALDSTDQIFPNFLLNELVSK----------------YKNSLENKLRSSSLLQHPDLASFRNALSVNSDSMSLPDVELMLKLLETKKQQLAANSLL---------------AQKQLLREFLQHLKKQKDEQLFTLQKEAAVIQGDLD 1969          

BLAST of EMLSAG00000000227 vs. C. finmarchicus
Match: gi|592940826|gb|GAXK01017727.1| (TSA: Calanus finmarchicus comp33124_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 52.7582 bits (125), Expect = 1.937e-6
Identity = 45/180 (25.00%), Postives = 70/180 (38.89%), Query Frame = 0

Query:   16 HYFQGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREIL-QLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDH 194
            H     + YD+ +++ +   C ICL     P  T C H FC +CI ++L    +CP     L E    P ++  + +L +L + CP  D                 SCK           EN  R            +L+DH        +  C   +VAC F  AGC  +   ++++DH
Sbjct: 2854 HSHHEPHIYDYHEEVDEDLMCQICLQPFVSPTDTPCSHTFCQACISSYLRVNASCPICRKPLNEMTCTPSNLVLKRLLDKLSVICPNSD-----------------SCK-----------ENSQR-----------GILEDHL-------KYRCDGTLVACQFAGAGCIFRGPVKKMKDH 3255          

BLAST of EMLSAG00000000227 vs. C. finmarchicus
Match: gi|592940827|gb|GAXK01017726.1| (TSA: Calanus finmarchicus comp33124_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 52.7582 bits (125), Expect = 1.943e-6
Identity = 45/180 (25.00%), Postives = 70/180 (38.89%), Query Frame = 0

Query:   16 HYFQGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREIL-QLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDH 194
            H     + YD+ +++ +   C ICL     P  T C H FC +CI ++L    +CP     L E    P ++  + +L +L + CP  D                 SCK           EN  R            +L+DH        +  C   +VAC F  AGC  +   ++++DH
Sbjct: 2854 HSHHEPHIYDYHEEVDEDLMCQICLQPFVSPTDTPCSHTFCQACISSYLRVNASCPICRKPLNEMTCTPSNLVLKRLLDKLSVICPNSD-----------------SCK-----------ENSQR-----------GILEDHL-------KYRCDGTLVACQFAGAGCIFRGPVKKMKDH 3255          

BLAST of EMLSAG00000000227 vs. C. finmarchicus
Match: gi|592866821|gb|GAXK01090741.1| (TSA: Calanus finmarchicus comp5828756_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 40.4318 bits (93), Expect = 1.674e-3
Identity = 15/38 (39.47%), Postives = 21/38 (55.26%), Query Frame = 0

Query:   34 FECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCP 71
             +CPIC+        T+CGH FC  CI+ W  + + CP
Sbjct:  179 LQCPICMEVYIMATSTNCGHTFCQDCIVDWRKKNKLCP 292          

BLAST of EMLSAG00000000227 vs. L. salmonis peptides
Match: EMLSAP00000000227 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1031:39445:42825:1 gene:EMLSAG00000000227 transcript:EMLSAT00000000227 description:"maker-LSalAtl2s1031-augustus-gene-0.52")

HSP 1 Score: 947.192 bits (2447), Expect = 0.000e+0
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query:    1 MDKETGRSGTKGIPKHYFQGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFTMD 453
            MDKETGRSGTKGIPKHYFQGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFTMD
Sbjct:    1 MDKETGRSGTKGIPKHYFQGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFTMD 453          

BLAST of EMLSAG00000000227 vs. L. salmonis peptides
Match: EMLSAP00000009169 (pep:novel supercontig:LSalAtl2s:LSalAtl2s579:316079:317682:-1 gene:EMLSAG00000009169 transcript:EMLSAT00000009169 description:"maker-LSalAtl2s579-augustus-gene-3.10")

HSP 1 Score: 56.6102 bits (135), Expect = 7.269e-9
Identity = 65/270 (24.07%), Postives = 115/270 (42.59%), Query Frame = 0

Query:  184 IKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE--YLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKE----IPEGSKA-FERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILK 446
            + I R +L+ H+  +   H+S L  +       Y       +S+  E+  S  KE +      L L  S +H  +  IK L            Q+E+                  A   Y NG  +WK+ +     ++ +        S  F+TS  GYK+     +N   G E  ++ +++ L+ GD DS L WPF+  +S + + + +EN  + +      IP+ + A F +P  +++  GFGF +F+    L S  +I  +N + +K
Sbjct:  264 LGIPREDLERHLGDECGKHMSALLCE-------YHEAGCRFRSTDPESLESHLKEKVTFH---LDLMASLVHKQKGQIKQLLN----------QVEL------------------AHTSY-NGILLWKIKNISTKMQESKASEGLEFVSVPFFTSQCGYKLXASLFLNGNGGGEGSHMSIYIKLLPGDYDSILKWPFKHTVSFSLLDQ-NENKKKVVNVVESFIPDPTWANFCKP--QKDQLGFGFPKFVSHTMLNSRDYIK-DNCIFIK 490          

BLAST of EMLSAG00000000227 vs. L. salmonis peptides
Match: EMLSAP00000006113 (pep:novel supercontig:LSalAtl2s:LSalAtl2s332:452120:457604:1 gene:EMLSAG00000006113 transcript:EMLSAT00000006113 description:"augustus_masked-LSalAtl2s332-processed-gene-4.0")

HSP 1 Score: 51.6026 bits (122), Expect = 3.741e-7
Identity = 28/74 (37.84%), Postives = 39/74 (52.70%), Query Frame = 0

Query:   34 FECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLG---EGDIFPDSMAHREILQLMITCPQKDH 104
            F CP+C    ++PH T CGH FCYSC+   L     CP   CS     + DI+P+   ++ I +L     QKD+
Sbjct:   10 FSCPVCFDILKEPHMTRCGHSFCYSCLNKSLKIKPQCP--KCSFDLHPDKDIYPNFTLNQIISKL-----QKDN 76          

BLAST of EMLSAG00000000227 vs. L. salmonis peptides
Match: EMLSAP00000012664 (pep:novel supercontig:LSalAtl2s:LSalAtl2s95:15959:19070:1 gene:EMLSAG00000012664 transcript:EMLSAT00000012664 description:"maker-LSalAtl2s95-augustus-gene-0.22")

HSP 1 Score: 49.6766 bits (117), Expect = 7.221e-7
Identity = 31/107 (28.97%), Postives = 50/107 (46.73%), Query Frame = 0

Query:   21 GYDYD-FVQQLPKRFECPICL-LCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREIL-QLMITCPQKDHGCGAVIHLSDVZNHLRSCKY 124
            G++ D F   + + F C IC  + +       C H FC  CI  WL     CP D   L   D+ P     + +L +L+I C  KD  C  ++ L  + +H++ C++
Sbjct:    2 GFEVDRFEGDVSEEFFCSICSGILEEAIQAPGCEHCFCSLCIKQWLXRHPXCPVDRSPLSLHDLKPIPRVLKNLLAKLLIRCGHKD--CAKLVKLEQLSSHMKDCEF 106          

BLAST of EMLSAG00000000227 vs. SwissProt
Match: gi|122146515|sp|Q3ZCC3.1|TRAF6_BOVIN (RecName: Full=TNF receptor-associated factor 6; AltName: Full=E3 ubiquitin-protein ligase TRAF6)

HSP 1 Score: 214.542 bits (545), Expect = 1.712e-62
Identity = 158/504 (31.35%), Postives = 240/504 (47.62%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT------QERSSKKHEN-------PSRSSDSNTCPSCGNVLDDHEITQ----------------------GQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNE---MLQEKLKKVM------CSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVRE----ISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D  +G + T  +   + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CII  + + G  CP DN  L E  +FPD+ A REIL LM+ CP  + GC   + L  ++ H   C++        Q    K H N       P R      C +     +D EI +                           L CP A V C F++ GC  K+ R  L  H++  T  H       +R + Q   T SL   + V +     +     +R S  R   S +H+ Q+ I+ L  R+V  + +  +L  + E   M   +LK+ +       +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L + +    Y+ +FVH M+G+ DS L WPF+G I LT + +    + +N  E +   PE   AF+RP   RNP GFG+  F+ + AL    FI  ++TL+++ E+ T
Sbjct:   35 DSVSGTASTVTLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCP--NEGCLHKMELRHLEEHQAHCEFALMSCPQCQRPFQKCHLNIHILKECPRRQVPCENC-AVSMAFEDKEIHEQNCPLANVICEYCNTMLIREQMPNHYDLDCPTAPVPCTFSAFGCHEKMQRNHLARHLQENTQSH-------MRMMAQAVQTLSLAV-APVPQCTMPLYDSVPPTRPSSGR--HSEVHNFQETIQQLEGRLVRQDHQIRELTAKMETQSMYVNELKRTIRTLEDKVAEIEAQ--QCNGIYIWKIGNFGMHLKSQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPSAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAAVRQNHEEIMDAKPE-LLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIK-DDTLLVRCEVST 521          

BLAST of EMLSAG00000000227 vs. SwissProt
Match: gi|30580642|sp|Q9Y4K3.1|TRAF6_HUMAN (RecName: Full=TNF receptor-associated factor 6; AltName: Full=E3 ubiquitin-protein ligase TRAF6; AltName: Full=Interleukin-1 signal transducer; AltName: Full=RING finger protein 85)

HSP 1 Score: 213.001 bits (541), Expect = 4.944e-62
Identity = 151/496 (30.44%), Postives = 231/496 (46.57%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT------QERSSKKHEN-------PSRSSDSNTCPSCGNVLDDHEI----------------------TQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSR-DSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVRE----ISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D   G + T  +   + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CII  + + G  CP DN  L E  +FPD+ A REIL LM+ CP  + GC   + L  +++H   C++        Q    K H N       P R    + C +     +D EI                             L CP A + C F++ GC  K+ R  L  H++  T  H+ +L   +  +    D+  ++   +  ET      EG L R D  +R   + + +    + +L R + +LE K                    +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L   +    Y+ +FVH M+G+ DS L WPF+G I LT + +    + +N  E +   PE   AF+RP   RNP GFG+  F+ + AL    FI  ++TL+++ E+ T
Sbjct:   34 DSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCP--NEGCLHKMELRHLEDHQAHCEFALMDCPQCQRPFQKFHINIHILKDCPRRQVSCDNCAA-SMAFEDKEIHDQNCPLANVICEYCNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQ--LEGRLVRQDHQIRELTAKMETQSMYVSELKRTIRTLEDK-------------------VAEIEAQ--QCNGIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPE-LLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIK-DDTLLVRCEVST 501          

BLAST of EMLSAG00000000227 vs. SwissProt
Match: gi|288558832|sp|P70196.2|TRAF6_MOUSE (RecName: Full=TNF receptor-associated factor 6; AltName: Full=E3 ubiquitin-protein ligase TRAF6)

HSP 1 Score: 212.231 bits (539), Expect = 1.044e-61
Identity = 146/503 (29.03%), Postives = 230/503 (45.73%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKY------------------------------------ITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGI-------LSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVRE----ISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D  +G + T  +   + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CII  + + G  CP DN  L E  +FPD+ A REIL L + CP K  GC   + L  +++H   C++                                    ++     K+  + S    +  C  CG +L   ++       L CP A + C F+  GC  K+ R  L  H++  T  H+ LL   +  +          + S        +++E I       + +D  +R   + + +    + +L R + +LE K                    +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L   +    Y+ +FVH M+G+ DS L WPF+G I LT + +    I +N  E +   PE   AF+RP   RNP GFG+  F+ + AL  G FI  ++TL+++ E+ T
Sbjct:   34 DSVSGSASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNK--GCLQKMELRHLEDHQVHCEFALVNCPQCQRPFQKCQVNTHIIEDCPRRQVSCVNCAVSMAYEEKEIHDQSCPLANIICEYCGTILIREQMPNHYD--LDCPTAPIPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLALRPCDAASPSRGCRPEDPNYEETIKQLESRLVRQDHQIRELTAKMETQSMYVGELKRTIRTLEDK-------------------VAEMEAQ--QCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEALIRQNHEEVMDAKPE-LLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIK-DDTLLVRCEVST 509          

BLAST of EMLSAG00000000227 vs. SwissProt
Match: gi|288559146|sp|B6CJY4.1|TRAF6_CERAT (RecName: Full=TNF receptor-associated factor 6; AltName: Full=E3 ubiquitin-protein ligase TRAF6)

HSP 1 Score: 211.46 bits (537), Expect = 1.649e-61
Identity = 148/496 (29.84%), Postives = 230/496 (46.37%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT------QERSSKKHEN-------PSRSSDSNTCPS-----------------------CGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSR-DSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSK---AFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D   G + T  +   + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CII  + + G  CP DN  L E  +FPD+ A REIL LM+ CP  + GC   + L  +++H   C++        Q    K H N       P R    + C +                       C  +L   ++       L CP A + C F++ GC  K+ R  L  H++  T  H+ +L   +  +    D+  ++   +  ET      EG L R D  +R   + + +    + +L R + +LE K                    +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L   +    Y+ +FVH M+G+ DS L WPF+G I LT + +      +  +EI +      AF+RP   RNP GFG+  F+ + AL    FI  ++TL+++ E+ T
Sbjct:   34 DSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCP--NEGCLHKMELRHLEDHQAHCEFALVDCPQCQRPFQKFHINIHILKDCPRRQVSCDNCAALVAFEDKEIHDQNCPLANVICEYCNTILIREQMPNHYD--LDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLAQAVHSLSLIPDSGYVSEVRNFQETIHQ--LEGRLVRQDHQIRELTAKMETQSTYVSELKRTIRTLEDK-------------------VAEIEAQ--QCNGIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPDLLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIK-DDTLLVRCEVST 501          

BLAST of EMLSAG00000000227 vs. SwissProt
Match: gi|288559147|sp|B6CJY5.1|TRAF6_MACMU (RecName: Full=TNF receptor-associated factor 6; AltName: Full=E3 ubiquitin-protein ligase TRAF6)

HSP 1 Score: 211.46 bits (537), Expect = 2.012e-61
Identity = 148/496 (29.84%), Postives = 230/496 (46.37%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQG--GYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT------QERSSKKHEN-------PSRSSDSNTCPS-----------------------CGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSR-DSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSK---AFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D   G + T  +   + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CII  + + G  CP DN  L E  +FPD+ A REIL LM+ CP  + GC   + L  +++H   C++        Q    K H N       P R    + C +                       C  +L   ++       L CP A + C F++ GC  K+ R  L  H++  T  H+ +L   +  +    D+  ++   +  ET      EG L R D  +R   + + +    + +L R + +LE K                    +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L   +    Y+ +FVH M+G+ DS L WPF+G I LT + +      +  +EI +      AF+RP   RNP GFG+  F+ + AL    FI  ++TL+++ E+ T
Sbjct:   34 DSVGGTASTGNLSSSFMEDIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCP--NEGCLHKMELRHLEDHQAHCEFALVDCPQCQRPFQKFHINIHILKDCPRRQVSCDNCAALVAFEDKEIHDQNCPLANVICEYCNTILIREQMPNHYD--LDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLAQAVHSLSLIPDSGYVSEVRNFQETIHQ--LEGRLVRQDHQIRELTAKMETQSTYVSELKRTIRTLEDK-------------------VAEIEAQ--QCNGIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPDLLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIK-DDTLLVRCEVST 501          

BLAST of EMLSAG00000000227 vs. SwissProt
Match: gi|288559149|sp|B5DF45.1|TRAF6_RAT (RecName: Full=TNF receptor-associated factor 6; AltName: Full=E3 ubiquitin-protein ligase TRAF6)

HSP 1 Score: 210.69 bits (535), Expect = 4.152e-61
Identity = 147/482 (30.50%), Postives = 225/482 (46.68%), Query Frame = 0

Query:   21 GYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT----------QERSSKKH---ENPSRSSD----------------SNTCP-------SCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGI-------LSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVRE----ISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CI   + + G  CP DN  L E  +FPD+ A REIL L + CP K  GC   + L  +++H   C++            Q+    KH   + P R                     +CP        CG +L   ++       L CP A V C F+  GC  K+ R  L  H++  T  H+ LL   +  +  +      ++ S        +++E +       + +D  +R   + + +    + +L R + SLE K                    +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L   +    Y+ +FVH M+G+ DS L WPF+G I LT + +    I +N  E +   PE   AF+RP   RNP GFG+  F+ + AL  G FI  ++TL+++ E+ T
Sbjct:   55 GYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACITKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNK--GCVQKMELRHLEDHQVHCEFALVICPQCQRFFQKCQINKHIIEDCPRRQVSCVNCAVPMPYEEKEIHDQSCPLANIICEYCGTILIREQMPNHYD--LDCPTAPVPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLSLRPCDASSPSRGCRPEDPNYEETVKQLEGRLVRQDHQIRELTAKMETQSMHVSELKRTIRSLEDK-------------------VAEMEAQ--QCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAVIRQNHEEVMDAKPE-LLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIK-DDTLLVRCEVST 509          

BLAST of EMLSAG00000000227 vs. SwissProt
Match: gi|288559148|sp|A7XUJ6.1|TRAF6_PIG (RecName: Full=TNF receptor-associated factor 6; AltName: Full=E3 ubiquitin-protein ligase TRAF6)

HSP 1 Score: 210.305 bits (534), Expect = 6.110e-61
Identity = 157/503 (31.21%), Postives = 241/503 (47.91%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQ-----ERSSKK--------HENPSRS-----------------SDSN------TCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHST-QKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVM------CSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVRE----ISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D  +G + T  +   + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CII  + + G  CP DN  L E  +FPD+ A REIL L + CP  + GC   + L  +++H   C++        +R  +K         E P R                   D N       C  C  VL   ++       L CP A V C F++ GC  K+ R  L  H++  T  H+ ++   L+ +       SL   + V +     +    LSR S    S  N   T Q+L   L R+   + +   ++E ++ M   +LK+ +       +E EAQ   CNG ++WK+ +F    K    +   V++S GFYT   GYK+C+R ++ L   +    Y+ +FVH M+G+ DS L WPF+G I LT + +    I +N  E +   PE   AF+RP   RNP GFG+  F+ ++AL    FI  ++TL+++ E+ T
Sbjct:   35 DGVSGTASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCP--NVGCLHKMELRHLEDHQAHCEFALMNCPQCQRPFQKCQLNIHILKECPRRQVSCVNCAVSMAFEDKEIHDQNCPLANVICEYCNTVLIREQMPNHYD--LDCPTAPVPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMMAQALQGL-------SLAV-APVPQRDMLPYDSSPLSRISSGCCSDQNFQETIQQLEGRLVRQDHQIRELTAKMETQS-MYVSELKRTIRSLEDKVAEIEAQ--QCNGIYIWKIGNFGMHLKSQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPIRQNHEEIMDAKPE-LLAFQRPTIPRNPKGFGYVTFMHLDALRQRTFIK-DDTLLVRCEVST 520          

BLAST of EMLSAG00000000227 vs. SwissProt
Match: gi|82236068|sp|Q6DJN2.1|TRF6B_XENLA (RecName: Full=TNF receptor-associated factor 6-B; AltName: Full=E3 ubiquitin-protein ligase TRAF6)

HSP 1 Score: 209.534 bits (532), Expect = 2.096e-60
Identity = 137/483 (28.36%), Postives = 225/483 (46.58%), Query Frame = 0

Query:   21 GYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNT----------CPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSA---------------------------GCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILS----------RDSDLRLSGSNLHST-QKLIKDLFRRVVSLEQKNCQLEIR----NEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSK---AFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLI 444
            GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CI+  L + G  CP DN  L E  +FPD+ A REIL L + CP +  GC   + L  ++ HL  C++ + E S  +   P    + +           C +CG  +   +++   G+ L+CP A V C +                              GC  K+ R +L  H++  T  H+ +++  LR    ++ T   +  S +     + F+   LS             D      NL  T ++L   L R+   + +   ++E +    NE L+  ++ +     +  G +CNG F+WK+  F  L K    +   V++S GFYT   GYK+CLR ++ L + +    Y+ +FVH M+G+ DS L WP  G I L+ + +      +  +E+ +      AF+RP ++RNP GFG+  F+ +N L    ++  +   +
Sbjct:   57 GYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIVKSLRDAGHKCPVDNEILMENQLFPDNFAKREILSLKVKCPSQ--GCTETMELRHLERHLGQCQFASVECSQCQGSFPKSRLEKHMEHECGRRKIFCDNCGLAMAYEDMS---GHELICPLAYVTCEYCQTNLIREQMPSHYSMDCTMAPIPCMYYEFGCTEKMQRNDLARHLQDFTQAHMRMMFQTLR----SFSTTPTSHISDISFCDPNQFEPVPLSVAPAHPSHMPSHQDCSQETRNLRETIEQLEGRLVRQDHQIRELIAKMETQCTYVNE-LKHTIRSLEDKLLDVDGHHCNGVFIWKIKGFSGLQKTQEEEKPVVIHSPGFYTGKPGYKLCLRLHLQLPSAQRCANYISLFVHTMQGEYDSLLPWPLHGTIRLSILDQSEGAIIQDQEEVMDTKPDLLAFQRPTTQRNPKGFGYVTFMHLNTLKQRQYVKNDTLFV 529          

BLAST of EMLSAG00000000227 vs. SwissProt
Match: gi|288559206|sp|Q6IWL4.2|TRAF6_DANRE (RecName: Full=TNF receptor-associated factor 6; AltName: Full=E3 ubiquitin-protein ligase TRAF6)

HSP 1 Score: 207.994 bits (528), Expect = 5.521e-60
Identity = 148/478 (30.96%), Postives = 225/478 (47.07%), Query Frame = 0

Query:   19 QGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT------QERSSKKHENPSRSSDS----NTCPSCGN-----VLDDHEITQGQGNRLVCPY--------------------AIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLR-KIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEM-------LQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNG---EEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAEL 449
            Q GYD +F   L  ++ECPICL+  R   QT CGHRFC SCI   + + G+ CP DN  L E  +FPD+ A REIL L + C   + GC   + L  ++ HL  C++ T      QE     H +  +S        TCP C       V  +HE      N  VC Y                    A VAC F++ GC  K+ R EL  H++  T  H+  + + LR +   N   PS     S D+  +S+       R  D       L + ++ + +L  R+V  +Q+  +L I N+        L+ KL  +  S  E + +   G +VW++ +F    +        V++S  FYT   GYK+CLR ++   +      ++ +FVH M+G+ DS LSWP +G I L  + ++       + E     +AF+RP   RNP GFG+  F+ + AL   GF+  E+ L+++ E+
Sbjct:   54 QQGYDVEFDPPLESKYECPICLMGLRSAVQTPCGHRFCDSCIRKSIRDTGQKCPVDNEVLLEEQLFPDNFAKREILSLTVKC--SNFGCSEKMELRQLEKHLSQCRFATAPCPQCQESVPISHLDEHKSQHCLQRIMTCPDCAGSFVYAVKQNHEQFCPFAN-TVCEYCEMELIRDQLALHCDTDCLKAPVACTFSTFGCREKMTRNELAQHMQEFTQMHMRYMAEFLRSQTLNNCTMPSAAAHLSSDDRGASA-------RSPDSCQCKQELLNLRETVLELEGRLVRQDQQIRELCIHNDTQKNQVTELRRKLVSLEESTRELEAQQYQGIYVWRVENFSHHLRNQEAGQPIVLHSPPFYTGRPGYKLCLRLHLQTPSAPRCSNFISLFVHTMQGEFDSQLSWPLQGTIRLAVLDQVEGQHHIEVMETKPDLQAFQRPTVMRNPKGFGYVTFLHLQALRQRGFVK-EDVLLVRCEV 520          

BLAST of EMLSAG00000000227 vs. SwissProt
Match: gi|123916477|sp|Q3MV19.1|TRF6A_XENLA (RecName: Full=TNF receptor-associated factor 6-A; AltName: Full=E3 ubiquitin-protein ligase TRAF6)

HSP 1 Score: 202.986 bits (515), Expect = 4.677e-58
Identity = 144/507 (28.40%), Postives = 224/507 (44.18%), Query Frame = 0

Query:    3 KETGRSGTKGIPKHYFQG-------GYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWL-NEGRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSS----------KKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSA---------------------------GCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILS----------RDSDLRLSGSNLHST-QKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSE---FEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSK---AFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLI 444
            KE   S + G+P    Q        GYD +F   L  ++ECPICL+  R+  QT CGHRFC +CI+  L N G  CP DN  L E  +FPD+ A REIL L + CP  + GC   + L  +++HL  C++ + E S            KH           C +CG  +   + +   G+ L+CP A V C +                              GC  K+ R  L  H++  T  H+ ++   LR    ++ T   +  S +     + F+   LS             D      NL  T ++L   L R+   + +   ++E +   + E    +   E    +     CNG F+W++  F  L K    +   V++S GFYT   GYK+CLR ++ L + +    Y+ +FVH M+GD DS L WP +G I L+ + +      +   E+ +      AF+RP + RNP GFG+  F+ +N L    ++  +  L+
Sbjct:   32 KEDADSPSAGLPSGTTQNLDVEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACILKSLRNAGHKCPVDNEILMEKQLFPDNFAKREILSLRVKCP--NLGCTDTMELRHLEHHLVQCQFASVECSQCQGSFLKLLLDKHMEHECGRRRTFCDNCGLAMAYEDKS---GHELICPMAYVTCDYCQTNLIREQMPAHYSMDCTMAPIPCMYCEFGCREKMQRHNLAGHLQDFTQAHMRMMAQTLR----SFSTTPTSHISDISFCDPNQFEPVPLSVAPAHPSHMPSQQDCSQETRNLRETVEQLEGRLVRQDHQIRELIAKMETQCTYVNELKHTIHSLEDKVADMDAHRCNGVFIWRIKGFSGLQKSQEEEKPVVIHSPGFYTGDPGYKLCLRLHLQLPSAQRCANYISLFVHTMQGDYDSLLPWPLQGTIRLSILDQSESTARQDQVEVMDTKPDLLAFQRPTAPRNPKGFGYVTFMHLNTLKQRQYVKNDTLLV 529          

BLAST of EMLSAG00000000227 vs. Select Arthropod Genomes
Match: gb|EFA02851.1| (TNF-receptor-associated factor 2 [Tribolium castaneum])

HSP 1 Score: 221.09 bits (562), Expect = 9.248e-67
Identity = 137/417 (32.85%), Postives = 204/417 (48.92%), Query Frame = 0

Query:   33 RFECPICLLCQRDPHQTSCGHRFCYSCIITWLN-EGRTCPDDNCSLGEG-DIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRR-ELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREIS--ENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLI 444
            RFECPICL   RDP  TSCGHRFC SC+ +WL  E  +CP DN  L +G DIFPD+   REI Q    CP    GC   +   DV+ HL +C+Y       K  E P                                   + C F   GC  K     ELQ H++     H++LL        Q Y   ++ + S+      ++F +     D+ +    SN  +   L+K L+ + V LEQK  + +I    + E++  +  S  +   RYCNG ++W   DF      MR D   + YS GFYT+  GY++C+R N++      Y+ + +H+M+ ++D+AL WPF G++++T V  +    N  ET+   PE   AF++P+ + NP GFG+TEF  +  L+S G++   + LI
Sbjct:   25 RFECPICLAWLRDPVLTSCGHRFCRSCMDSWLERENPSCPIDNTKLEKGVDIFPDNFTRREISQQKTKCPNLVRGCLEELSPLDVETHLLTCQY-------KPPELPENEK-------------------------------LRCAFVEFGCEDKFEDEPELQRHLEQYIHKHLNLL-------SQAYSKFTINSPSTSAIAQQANFWDPPSKNDTSV---SSNHDNVNGLLKALYEKAVLLEQKTREQDIIIANMSEQISSLNLSMSKMHLRYCNGCYLWYFNDFKNKINAMRNDSRIMHYSPGFYTAANGYRLCIRLNLS-PKDPNYIAILIHVMKTEHDNALDWPFSGRLTITLVHPLQPFHNIKETMMTRPELD-AFKKPVQDMNPKGFGYTEFALIEELFSQGYVENNHLLI 391          

BLAST of EMLSAG00000000227 vs. Select Arthropod Genomes
Match: XP_624207.3 (PREDICTED: TNF receptor-associated factor 6 [Apis mellifera])

HSP 1 Score: 198.364 bits (503), Expect = 8.722e-58
Identity = 131/418 (31.34%), Postives = 190/418 (45.45%), Query Frame = 0

Query:   22 YDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLN-EGRTCPDDNCSL-GEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKI-MRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCS----EFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREI-SENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNAL 431
            YD    + L  RFECPICL   RDP  TSCGH+FC  CI TWL  EG  CP D+  L  E D+F D    REI Q    CP +  GC   +   D++ H+  C Y                    T P   NV                      CPF   GC       ++L  H++   + H++++   L +I          +Q++++  A+ S       + +            Q+L+K+L+ R+V LEQ+N +L I     + +L  +  S    + E   R CNG ++W+L  F E  + M  D   + YS GFYTS  GYK+C R N++     E+L + +H+M+ +ND  L WPF G +    V  + SE     I       +AF +PI E N   FG+TEF+R+  L
Sbjct:   20 YDDGIKESLEPRFECPICLTWLRDPVLTSCGHKFCSQCIYTWLQKEGACCPVDSKPLKSESDLFRDLYTSREISQQRTPCPYQQFGCEIKVSPVDMETHINECTY------------------KRTLPDSQNVY---------------------CPFKKVGCMETFPTEKDLHTHLEKNINIHLTMILKILPQIS--------VSQNNINAIAAESKLWDPPPKSTSCMEKSEPSLEWQQLLKNLYERIVVLEQQNRELSITVSNQKTQLTTLQTSLRFNQEEMFLRSCNGVYIWRLHSFHEKLESMMNDSLKMFYSPGFYTSPNGYKICARINVS-SKDPEFLSLLLHIMKSENDDGLDWPFNGTMYFVLVHPLNSERDIREITSSRPDLEAFRKPICELNKRSFGYTEFVRIRDL 389          

BLAST of EMLSAG00000000227 vs. Select Arthropod Genomes
Match: AAF46338.1 (TNF-receptor-associated factor 6 [Drosophila melanogaster])

HSP 1 Score: 187.193 bits (474), Expect = 3.922e-53
Identity = 132/441 (29.93%), Postives = 205/441 (46.49%), Query Frame = 0

Query:   16 HYFQGGYDYDFVQQ---LPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEG-RTCPDDNCSL-GEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENF--FETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAEL 449
            H++  G D    Q+   L  R+EC IC+    +P  TSCGHRFC SC+  W+ +  + CP DN  L  E DIFPD+   REI QL   CP    GC  V    ++  HL SC Y  Q+   ++                                  CP+A + C F       +    +L++H+K+   HH+ L+   L+  QQ   T   T Q     T+ ++ + G   +        +N    +++++ +++R+V LEQ+  + E R E +Q++L+ +   +     RY NG  VW++     L  ++R + +  VYS   YTS  GYK C R NI        L + VHLM+ +ND  L WPF+G+I L  V          +TI   PE   AF +P    +  GFGF E+  ++ +   GF   ++ L++K E+
Sbjct:   81 HHYAPGSDTSGEQEEELLDSRYECAICIDWLNEPVLTSCGHRFCRSCLTAWMQKNNQCCPMDNKRLSAEHDIFPDNYTRREIEQLKRDCPNSSLGCSVVASPIELHRHLPSCPYRRQQEPQEEK---------------------------------CPFAKIKCDFVG-----RPETNQLEEHLKADMPHHMQLM---LQAFQQ---TAIATWQPHKPSTSGAAVENGHGQQQLPPPPQYAN-GVDEQIVQTMYQRIVVLEQRTREQETRLENMQKQLR-LARQQAPVDPRYSNGTIVWRIEQLGALVARLRANANNQVYSHECYTSPHGYKFCARLNIQ-PRKPHVLSLHVHLMQSENDYHLDWPFKGRIKLCMVHPADATLSQHDTIMTKPE-ILAFHKPREAISTRGFGFLEYANISNIIQLGFC-ADDRLLIKIEI 472          

BLAST of EMLSAG00000000227 vs. Select Arthropod Genomes
Match: EAA07925.6 (AGAP003004-PA [Anopheles gambiae str. PEST])

HSP 1 Score: 181.415 bits (459), Expect = 1.357e-51
Identity = 131/424 (30.90%), Postives = 192/424 (45.28%), Query Frame = 0

Query:   30 LPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRT-CPDDNCSLG-EGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTA--VREISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAEL 449
            L  R+ECPIC     +P  T CGHRFC  CI  WLNE  + CP DN  L  + DIFPD+   REI Q+   CP    GCG  +  +D++ HLR C +    +S                                     CP+A V C F +   A       +  HI S    H+           Q   +P ++  +  D  A +S  E                 ST +L++ ++ R+V LEQ+   L I+    + +L K+     E   RY  G  +WKL DF    + M  + + + YS   YTS  GYK C R N++    ++ +G+ VHLM+ +ND  L WPF+G+I +T   VR    +  +TI   PE   AF RP  + +P GFGF EF ++  + +    N  NT+++K ++
Sbjct:   49 LEARYECPICYCWLNEPILTKCGHRFCRKCITDWLNEKNSNCPLDNEPLDIKCDIFPDNCTRREISQIKKPCPNSMRGCGEQLSPTDIDTHLRQCPFAMSRQSQP-----------------------------------CPFARVKCKFVAPDEA------AMSAHIASDCQQHL-----------QVARSPCVSALTFWDPPAKNSVPE----------------MSTNELVRSMYERIVILEQEVHILGIKLSKQEIQLTKL---NQEVDPRYSGGVLLWKLDDFSNKIESMVANSNCMFYSSEAYTSPHGYKFCARINVS-PRTKDSIGLHVHLMQSENDYHLEWPFKGRIKITLLNVRSPELSQHDTIMSKPE-ILAFHRPNQDISPRGFGFLEFAKIKDILAKFADN--NTVVIKIQM 397          

BLAST of EMLSAG00000000227 vs. Select Arthropod Genomes
Match: EEB11705.1 (conserved hypothetical protein [Pediculus humanus corporis])

HSP 1 Score: 175.252 bits (443), Expect = 3.495e-49
Identity = 123/428 (28.74%), Postives = 207/428 (48.36%), Query Frame = 0

Query:   30 LPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWL-NEGRTCPDDNCSLGEG-DIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENF--FETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFTMD 453
            L ++FECPICL C ++P  TSCGHRFC SC+  WL ++G +CP D   L +  D+FPD    REIL     C      C   I LS+++ H+ S  + +   S K  E+                        G+G           CPF + GC + +    L+DH++   + H++L+ +    +++       T + S D  A  S       +  D             L++ L+ RVV LEQ+  +   + E L+ +L++      E   R+  G+++W++ DF +    M++    V +S+GFYTS  GYK+C R N++ V+  + L + +H+M+  +D  L WPF G+I    V          ETI   P+   +F++P  + N   FG+ +FI +N +++ G++  +++LI+K  +   D
Sbjct:   13 LDRQFECPICLSCLKEPVLTSCGHRFCSSCLNLWLESKGESCPVDGGILNKKLDLFPDHFTRREILNRTTICKL----CKLEIGLSEMDEHVSSHHHHSSRASKKVSED-----------------------NGKG----------CCPFGTFGCDVDVTENTLKDHLEKDLNLHLNLVANSHLNLREKLLKN--TDKMSDDVLAQESLMWEPQEKGKD--------RQNASLMRALYERVVVLEQRCHEQNAQMEKLKGRLEESNDRIREVSLRHACGQYLWRIDDFGKKIGSMKSGDKRVFFSEGFYTSPNGYKICGRINLSPVDS-DMLSLLIHIMQTPHDHTLVWPFTGRIQFAIVHPTDSKLTIAETIMSRPD-LDSFKKPARDINTKAFGYPQFISINKIFNNGYL-IDDSLIVKLHVQEAD 390          

BLAST of EMLSAG00000000227 vs. Select Arthropod Genomes
Match: gb|EEC15888.1| (Tnf receptor associated factor, putative [Ixodes scapularis])

HSP 1 Score: 83.9593 bits (206), Expect = 1.188e-16
Identity = 97/429 (22.61%), Postives = 178/429 (41.49%), Query Frame = 0

Query:   18 FQGGY-DYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQE----RSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRL-VCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLL-WDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVN--GEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEG-----SKAFERPISERNPTGFGFTEFIRVNAL 431
            F G Y   +F +++P    C +C     +   T C H FC  C     +E G TCP DN       ++ D+ +   +    + CP K +GC  V  L++++ H+ SC Y   +        KH++ + +     C          +   G+G+ + V P    A    +  C I  +R +L D  +   S   S   +D +R           +T ++V +T     K+  +  DS +    +N            +R+V+          + E +Q  LK  + S    + R+ + KFVW + +F +L +++        +S  FY  + GYK+ L  +    +  G+  L V+V++ +G  D  L WP++ + +   V + S  + +  + +PE         F+RP    N  G G+T  + +  +
Sbjct:   13 FVGEYTSIEFQEEVPIYRICSLCGAVPSELFLTKCSHFFCPKCFSKLKDEDGFTCPVDNTKSLPDQVYHDTTSANILQGFRVACPNKSNGCEYVGPLNNLKEHVSSCGYHGVQCGICMKELKHQDVA-AHLKMECQRSMQPQQAPQQEAGEGSAVAVYPREDGAMYTGAEACQISEVREKLDDVCREVKSLKDSFAEFDVIR-----------STVNAVQDTVKIEMKK--MREDSAI----AN------------KRIVT----------KIEKIQSYLKGRIDS---IEDRFRSSKFVWHIKNFSKLKEQVMDGKAKSFFSDDFYVGIQGYKMYLGGHFAEKDDKGKTCLSVYVYITKGPYDPTLEWPYKRRSTFVIVDQKSNTYHKIAEIVPEELGIDLEHCFQRPKDGEN-EGIGYTAIMSLEDI 397          

BLAST of EMLSAG00000000227 vs. Select Arthropod Genomes
Match: gb|KYB25183.1| (TNF receptor-associated factor 4-like Protein [Tribolium castaneum])

HSP 1 Score: 77.411 bits (189), Expect = 2.077e-14
Identity = 99/417 (23.74%), Postives = 166/417 (39.81%), Query Frame = 0

Query:   74 NCSLGE-GDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSK--------------KHENPSRSSDSNTCPS--CGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHV--------SLLWDKLRKIQQNYDTPSLTTQSSVD------ETASSSFKE----GILS---RDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKV--CLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLT-----AVREISENFFETIKEIPEGS-KAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLI 444
            N SL E   I+PD  + + I+  ++ C     GC     L  ++ HL SCK+     + K              K   P R +    C     G V ++H      GN   C Y  + C      C +K+ RR L  H  S+ S  +          + D L           +   +  D      E   +  KE     +LS   +D+  R  G   +  +K +++  ++ ++L    C +  + +     LK  +         Y +G  +WK+ DF E   + +      + S  FYTS +GYK+   L  N N    + ++ V++ ++ G+ D+ L WPF   +S T     +  E + N  E+   IP+ + K F+RP  E +  GFGF +F+    L    F+  +   I
Sbjct:   59 NSSLNEVNQIYPDPESEKAIMSSVVYCIHHKEGCKWSDELRKLKAHLNSCKHDAIPCTGKCGAQIPRVIMDEHLKTTCPQRRARCQFCNKEFTGLVFENH-----VGN---CGYEPLYC---ENKCGVKVARRHLSQHKVSECSKRLLPCRFCSKEFVADTLSAHHLKCGRVPVACPNRCDVNVLAKEDLETHLKEECTVSVLSCTYKDAGCRFKGPR-YLMEKHLEESSQQHLALV---CTVVTKQQHQITSLKNALS---RLSLNY-SGTLIWKISDFSEKMTEAKNKEGMELVSPPFYTSQYGYKLQASLFPNGNGAGEDSHISVYIKILPGEYDALLRWPFAHSVSFTLFDQSSCPEKACNIVESF--IPDPTWKNFQRPSKEPDSLGFGFPKFVSHEMLKKRHFMKDDTMFI 454          

BLAST of EMLSAG00000000227 vs. Select Arthropod Genomes
Match: XP_006572597.1 (PREDICTED: TNF receptor-associated factor 6-like [Apis mellifera])

HSP 1 Score: 64.6994 bits (156), Expect = 2.742e-12
Identity = 34/63 (53.97%), Postives = 37/63 (58.73%), Query Frame = 0

Query:   22 YDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLN-EGRTCPDDNCSLGEGDIF 83
            YD    + L  RFECPICL   RDP  TSCGH+FC  CI TWL  EG  CP D+  L    IF
Sbjct:   20 YDDGIKESLEPRFECPICLTWLRDPVLTSCGHKFCSQCIYTWLQKEGACCPVDSKPLKSEFIF 82          

BLAST of EMLSAG00000000227 vs. Select Arthropod Genomes
Match: XP_006559813.1 (PREDICTED: TNF receptor-associated factor 4 isoform X3 [Apis mellifera])

HSP 1 Score: 68.9366 bits (167), Expect = 8.184e-12
Identity = 87/407 (21.38%), Postives = 158/407 (38.82%), Query Frame = 0

Query:   83 FPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSK-----------KHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSS----VDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLK---KVMCSEFEAQGRYCN--------------GKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKV--CLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISL-----TAVREISENFFETIKEIPEGS-KAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAEL 449
            F D  A + I+  ++ C     GC     L  ++ HL +CK+     S+K            H   + +     C  C      H + +  G    C Y  + C      C +K+ RR L  H   + +  +       R   + +   +L    +             +  +L R+ DL +   + H T  L+   F+      + N +  + ++ L+E  K    +MCS    Q                   G  +WK+ D+     + +      + S  FYTS +GYK+   +  N N      ++ +++ ++ G+ D+ L WPF   +S      T V E + N  E+   IP+ + K F+RP  E +  GFGF  F+    +    F+  +NT+ ++ ++
Sbjct:   12 FQDLEAEKAIMGSIVYCIHHKDGCKWSDELRKLKAHLNTCKHDAVPCSNKCGAMIPRVLMEDHLKYTCAQRRARCDFCAKEFTGHTLEKHTGT---CGYEPLYC---ENKCGMKVQRRHLSQHKLGECAKRLV----ACRYCNKEFVFDTLGAHHAKCGRFPVACPHRCETAVLPRE-DLEVHLKD-HCTTHLLSCTFKDAGCRFKGN-RFSL-DKHLEESAKMHLSLMCSVVTKQQHQITSLKSAISKLSLNYTGTLIWKITDYSAKMSEAKAKEGMELVSPPFYTSQYGYKLQASVFLNGNGTGEGSHISIYIKILPGEYDALLRWPFSHSVSFTIFDQTVVAEKACNIVESF--IPDPTWKNFQRPSREPDSLGFGFPRFVSHEMVKKRHFVK-DNTMFIRVKV 401          

BLAST of EMLSAG00000000227 vs. Select Arthropod Genomes
Match: EEB14039.1 (TNF receptor-associated factor, putative [Pediculus humanus corporis])

HSP 1 Score: 68.9366 bits (167), Expect = 1.067e-11
Identity = 47/153 (30.72%), Postives = 78/153 (50.98%), Query Frame = 0

Query:  305 NGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE--YLGVFVHLMRGDNDSALSWPFRGQISLTAV-----REISENFFETIKEIPEGS-KAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAEL 449
            +G  +WK+ DF     + ++     + S  FYTS +GYK+   + +N     E  +L V++ ++ G+ D+ L WPF   +S T        E + N  E+   IP+ + K F RP  E +  GFGF  FI  + L+   FI  ++TL L+ ++
Sbjct:  297 SGTLIWKISDFTVKLSEAKSKDGVELVSPPFYTSQYGYKLQASAFLNGNGAGESTHLSVYIKILPGEYDALLRWPFSHSVSFTLFDQSPNTEKACNVVESF--IPDPTWKNFLRPSKEPDSLGFGFPRFISHDLLHKRYFIR-DDTLFLRVKV 446          

BLAST of EMLSAG00000000227 vs. nr
Match: gi|290463015|gb|ADD24555.1| (TNF receptor-associated factor 6 [Lepeophtheirus salmonis])

HSP 1 Score: 947.577 bits (2448), Expect = 0.000e+0
Identity = 451/453 (99.56%), Postives = 453/453 (100.00%), Query Frame = 0

Query:    1 MDKETGRSGTKGIPKHYFQGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFTMD 453
            MDKETGRSGTKGIPKHYFQGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGA+IHLSDV+NHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFTMD
Sbjct:    1 MDKETGRSGTKGIPKHYFQGGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAIIHLSDVENHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFTMD 453          

BLAST of EMLSAG00000000227 vs. nr
Match: gi|676448266|ref|XP_009051677.1| (hypothetical protein LOTGIDRAFT_208992 [Lottia gigantea] >gi|556109183|gb|ESO97835.1| hypothetical protein LOTGIDRAFT_208992 [Lottia gigantea])

HSP 1 Score: 233.417 bits (594), Expect = 1.083e-67
Identity = 145/473 (30.66%), Postives = 236/473 (49.89%), Query Frame = 0

Query:   21 GYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKY-----------ITQERSSKKHENPSRSSDSNTCPSCG-----NVLDDHE--------ITQGQGNRLV-----------CPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE--YLGVFVHLMRGDNDSALSWPFRGQISLTAVREIS-----ENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELF 450
            GYDY+FV     ++EC ICLL  R+P QT CGHRFC  CII WL E  +TCP DN  + E D+F D+ A REI    + CP K  GC  ++ L+ ++ H+  C +           I   R    H +        TC SC      N L  HE        + +    +L+           C   +V C F   GC  ++ R+ +++H++   S H+ +L  ++ +I +    P+    +  D  + S F+   L ++++LR S    H  +         +  L  K  + E  N  L++++K +     E  GR CNG + WK+ D+ +L ++ +      ++S  FY+S +GYK+C+R N+N V+  +  +L +F+H ++GD D  L WPF G+I L+ + + S      +  ET+   P  + AF++P + RN  GFG+ EF+ ++ L  G F+  + TLI+KA + 
Sbjct:   37 GYDYEFVPPPDSKYECVICLLVLREPQQTKCGHRFCKVCIIKWLRESNKTCPVDNAKIVESDLFADNFAKREIQNFNVRCPNKSQGCEKIVMLNLIQKHIEDCPFGLIPCSNNCSDILLRRDLNHHLSSVCCYRQITCHSCSRRLTYNDLQKHEFDCPEIIVVCEKCDKQLLRKWKQHHENTECLKTVVNCMFQPLGCPQQMERQTMEEHMEKGLSDHMKMLCSEVVQISRCLGLPTGQNYNPTDNGSISDFQS--LKKENNLRDSSLAEHGQE---------ISQLNDKFREYERINRDLKQRVKYLESCIDERDGRSCNGIYYWKIKDYSKLRREAQLGNMPAIHSPPFYSSFYGYKLCVRVNLNGVDSAKGSFLSIFIHFLQGDFDDLLDWPFAGRIILSVIDQNSTADYRSHIVETLIAKPTLA-AFQKPTTTRNHKGFGYMEFVPLHVLDKGQFLKND-TLIIKAHVI 496          

BLAST of EMLSAG00000000227 vs. nr
Match: gi|1130248582|ref|XP_019765776.1| (PREDICTED: TNF receptor-associated factor 6 [Dendroctonus ponderosae] >gi|478253622|gb|ENN73928.1| hypothetical protein YQE_09471, partial [Dendroctonus ponderosae] >gi|546683940|gb|ERL93680.1| hypothetical protein D910_10968 [Dendroctonus ponderosae])

HSP 1 Score: 230.335 bits (586), Expect = 1.586e-67
Identity = 154/423 (36.41%), Postives = 207/423 (48.94%), Query Frame = 0

Query:   33 RFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLG-EGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRR-ELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEG-ILSRDSDLRLSGSNL---HSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREIS--ENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKA 447
            RFECPICL   RDP  TSCGHRFC +CI  WL +   CP DN  L  + DIFPD+   REI Q    CP    GC   +   DV+ HL  C++   E                        L DHE               + C F + GC  K      LQ H++     H+ LL          Y   +L+  +S  E+ASS+  +       S    SGS L    + Q L+K L+ +++ LEQK  + +I    + E++     +    Q RYCNG F+W   DF    K MR +   + YS GFYTS +GYK+CLR N++      YL V +HLM+ ++D AL WPF G++++T V  I   +N  ET+   PE  +AF RPI + NP GFG+TEF  +  LY   FI T N LI+K 
Sbjct:   35 RFECPICLAWLRDPVLTSCGHRFCRNCIREWLEKEDACPVDNIKLNKDKDIFPDNFTRREISQQRTKCPNILRGCLKELSPLDVDTHLLVCEFRPPE------------------------LPDHE--------------KLRCVFVNVGCYEKFEDDPNLQRHLEQNVQQHLLLL-------SHAYSKATLSQNNS--ESASSALAQANFWDPPSKNEASGSPLGEDENLQNLLKALYEKIIFLEQKTREQDIIIANMSEQISNHSSTISNFQQRYCNGCFIWHFNDFKNKIKAMRENPRIMHYSPGFYTSAYGYKLCLRINLS-PKDLNYLAVLIHLMKSEHDQALDWPFCGRLTVTIVHPIQCYKNMKETMMSRPE-LQAFNRPIHDLNPKGFGYTEFASIEELYDQEFI-TSNQLIIKV 407          

BLAST of EMLSAG00000000227 vs. nr
Match: gi|817250525|emb|CRI06483.1| (tumor necrosis factor receptor-associated factor 6 [Scylla paramamosain])

HSP 1 Score: 233.802 bits (595), Expect = 6.980e-67
Identity = 162/535 (30.28%), Postives = 244/535 (45.61%), Query Frame = 0

Query:   21 GYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEG-RTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKY------------ITQERSSKKHEN--------------PSRSSDSN-----------TCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHV-----------SLLWDKLRKIQQNYDTPS---LTTQSSVDETASSS-------------------FKEGILSRD----SDLRLSGSNL-------------------------------HSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKV---MCSEF--EAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVN------GEEYLGVFVHLMRGDNDSALSWPFRGQISLT----AVREISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSG 434
            GYDY+FV  L  ++ECPICLL  R P QT+CGHRFC  CI   L+E  R CP DN  L E D+FPDS A RE+LQL + CP    GC  V+ L  +++H ++C +            + Q+      E+              P   ++             +C  CG V+   E+   + +   CP  +V CP+T  GC+ K +R +L  H+   T +H+           +L+W+  R       +PS    + Q S+    S++                   F    L  D    S L LS S L                               H  + ++KDL  + + + Q   Q+ I    LQ +++++   + +E    A+G+YC G++VW+L DFP    ++R     V++S GFYTS FGYK CLR N+   +      GEE+L +F+H M+G+ND  L WPF G I+ +           +  ET+   P+  ++F RP   RNP GFG+TEF  ++ +  G
Sbjct:   23 GYDYEFVPTLDPKYECPICLLALRAPLQTACGHRFCKDCIYNSLSESSRRCPIDNTPLKETDLFPDSCAEREVLQLRVRCPNTSLGCSQVLDLMYIEHHTQACSFQPVLCPNECSATVLQKELDHHLESQCLLRLKLCALCDLPYPFNEEQLHLATCAQVMVSCELCGCVVARGELPTHRSDS--CPRVVVGCPYTEHGCSHKTVRADLDAHLSQSTQYHLHLLSSAQKRVTALVWELSRSAGMWGQSPSGLGFSRQPSLRSQLSATSPSPDRQLGEGSCGERGLVFPSSALKLDGTPPSKLHLSFSQLAIVGEGATALEEGKTGEDGKAGLGKDLAGIHHQQEAILKDLCGKTLEVSQGLLQVNITLSNLQARVEEMEGWVAAELGEAARGKYCQGEYVWRLKDFPGTLAELREKPTRVIHSPGFYTSPFGYKFCLRLNLTRGSGKGERGGEEWLSLFIHGMQGENDDFLDWPFSGIITFSLLDCGAGRPKAHITETLHSSPD-RQSFSRPQMHRNPKGFGYTEFAPLSKVVEG 554          

BLAST of EMLSAG00000000227 vs. nr
Match: gi|1069788733|ref|XP_018319661.1| (PREDICTED: TNF receptor-associated factor 6 [Agrilus planipennis])

HSP 1 Score: 228.024 bits (580), Expect = 1.133e-66
Identity = 142/423 (33.57%), Postives = 216/423 (51.06%), Query Frame = 0

Query:   29 QLPK-RFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLG-EGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRR-ELQDHIKSQTSHHVSLLWDKLRK--IQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREIS--ENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLI 444
            Q+P+ RFECPICL   RDP  TSCGHRFC  CI +WL +   CP D   L  + DIFPD+   REI Q    CP    GC   +   D++ HL+ CKY   E                        L D+E               + C F + GC  K     E+++H++    +H++LL     K  I Q+  + +L  Q++  +  +     G++    D  ++ SN    + LIK L+ +++ LEQK  + +I    + E++            ++C G F+W + DF     +M  + H + YS GF TS FGYK+C+R N++  N   YL +F+H+M+ D+D++L WPF G+IS+T V  ++  +N  ET+   PE  +AF++P  E NP GFG+TEF  +  L S GF++ +  LI
Sbjct:   23 QVPEARFECPICLTWLRDPVLTSCGHRFCRKCIYSWLEKDTDCPVDKTKLNKDTDIFPDNFTRREISQQKTKCPNVVRGCLKELSPLDIEYHLQICKYRIPE------------------------LPDNE--------------KLRCNFVAVGCIEKFENEVEVKEHLEKNIHNHLNLLSQAYSKFIIDQSTSSNALAQQANFWDPPAKIGSNGLV----DEHVNSSN----ESLIKSLYEKIIILEQKTREQDIIIANMSEQISSCNLLISTVPTKFCFGSFLWPVADFKTKITRMSNNSHMMCYSPGFQTSPFGYKLCIRLNLSPKNP-NYLAIFIHMMKTDHDNSLDWPFNGKISITVVHPVNLEQNIKETMMTRPE-LEAFKKPKQELNPRGFGYTEFALIQDLCSEGFVDKDQLLI 397          

BLAST of EMLSAG00000000227 vs. nr
Match: gi|808724676|gb|AKD94181.1| (tumor necrosis factor receptor-associated factor 6 [Portunus trituberculatus] >gi|808724678|gb|AKD94182.1| tumor necrosis factor receptor-associated factor 6 [Portunus trituberculatus])

HSP 1 Score: 231.876 bits (590), Expect = 3.933e-66
Identity = 162/535 (30.28%), Postives = 241/535 (45.05%), Query Frame = 0

Query:   21 GYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEG-RTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKY-----------ITQERSSKKH---------------ENPSRSSDSN-----------TCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHV-----------SLLWDKLR-------------------KIQQNYDTPSLTTQSSVDETASSSFKEGILSRDSDLRLSGS-----------------------------------NLHSTQK-LIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKV---MCSEF--EAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNIN--------LVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLT----AVREISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSG 434
            GYDY+FV  L  ++ECPICLL  R P QT+CGHRFC  CI   L+E  R CP DN  L E D+FPDS A RE+LQL + CP    GC  V+ L  +++H ++C +              +R   +H               + P   +              +C  C  V+   E+   + +   CP  +V CP+   GC+ K +R +L  H+   T +H+           +L+W+  R                   + Q +  +PS   Q           +E  L   S L+L  S                                    +H  Q+ ++KDL  + + + Q   Q+ I    LQ ++++V   + +E    A+GRYC G++VW+L DFP    ++R     V +S GFYTS FGYK CLR N+            GEE+L +F+H M+G+ND+ L WPF G I+      +V +   +  ET++  P+  +AF RP + RNP GFG+TEF  V  +  G
Sbjct:   23 GYDYEFVPALDPKYECPICLLALRAPLQTACGHRFCKDCIYNSLSESSRRCPIDNTPLKETDLFPDSCAEREVLQLRVRCPNTSLGCTQVLDLMYIEHHTQACSFQPVLCPNECSVTVLQRELDQHLKSQCLLRLKLCVLCDLPYPFNQEQLHLATCAQVMVSCELCSAVVARGELPTHRSDS--CPRVVVGCPYAEHGCSHKTVRADLDAHLSQSTQYHLHLLSSAQKRVTALVWELSRSAGVWGSPSGLGFSRQPSLRSQLSATSPSPDRQLGESSCGERGERERGLVFPSPLKLDTSPKLHLSLSQLAIVGEGATALEEGKGGEDGKAGKDLAMHPQQEAILKDLCGKTLEMSQGLLQVNIGLSNLQARVEEVEGWVTAEGGEAARGRYCQGEYVWRLKDFPGTLAELREKPTRVFHSPGFYTSPFGYKFCLRLNLTRNGQGGQGGPAGEEWLSLFIHGMQGENDAFLDWPFAGVITFALLDCSVGKPKAHITETLQSSPD-RQAFSRPQTHRNPKGFGYTEFAPVRKVVDG 554          

BLAST of EMLSAG00000000227 vs. nr
Match: gi|701395653|ref|XP_009995570.1| (PREDICTED: TNF receptor-associated factor 6 [Chaetura pelagica] >gi|678112996|gb|KFU89323.1| TNF receptor-associated factor 6 [Chaetura pelagica])

HSP 1 Score: 228.409 bits (581), Expect = 2.493e-65
Identity = 160/499 (32.06%), Postives = 241/499 (48.30%), Query Frame = 0

Query:    8 SGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT----------QERSSKKHENPSRSSDSNTCPSCGNVL-----DDHEITQGQGNRLV-------------------CPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDS----DLRLSGSNLHST-QKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVM------CSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVREIS----ENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            SGT   P  + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC  CI+  + + G  CP DN  L E  +FPD+ A REIL LM+ CP K  GC   + L  +++H   C + T          Q+   K+H     S     CP+C   +     + H+ T    N                      CP   V C +++ GC  K+ R EL  H++  T  H       +R + Q++   S+T  + V      SF+  + S  S    D      N   T Q+L   L R+   + +   ++E +N  + E LK+ +       +E EAQ   CNG ++WK+ +F EL K    +   V++S GFYT   GYK+CLR +I L N +    ++ +FVH M+G+ DS L WPF+G I L+ + +      +N  E ++  PE   AF+RP   RNP GFG+  F+ +  L    FI  ++TL+++ E+ T
Sbjct:   40 SGTGNAPSSFTEETQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKGCIVKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCPNK--GCSMKMELRHLEDHQLQCDFSTMECPQCQGAFQKNRLKEHMTQECSRRQVCCPNCATSMAYEDKELHDQTCPLANVFCEYCNTVLIREQMPNHYDNDCPTVPVPCFYSAFGCPEKMQRNELARHMQEFTQVH-------MRMMAQSFQNISVTATNPVPFINGLSFEPALFSHVSHAAYDCNPEVENFKETIQQLEGRLVRQDHQIRELIAKMETQNTHMAE-LKRTIRNLEGKITEMEAQ--QCNGIYIWKIENFSELQKAQEEERPVVMHSPGFYTGKPGYKLCLRLHIQLPNAQRCANFISLFVHTMQGEYDSHLPWPFQGTIRLSILDQSEGPERQNHEEVMEAKPE-LLAFQRPTIHRNPKGFGYVTFMHLQTLKQRTFIK-DDTLLVRCEVLT 524          

BLAST of EMLSAG00000000227 vs. nr
Match: gi|699607763|ref|XP_009872433.1| (PREDICTED: TNF receptor-associated factor 6 [Apaloderma vittatum] >gi|677308741|gb|KFP91169.1| TNF receptor-associated factor 6 [Apaloderma vittatum])

HSP 1 Score: 227.639 bits (579), Expect = 5.361e-65
Identity = 165/509 (32.42%), Postives = 245/509 (48.13%), Query Frame = 0

Query:    3 KETGRSGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYIT----------QERSSKKH---ENPSRSS----------------DSNTCP-------SCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDS----DLRLSGSNLHST-QKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVM------CSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAV-------REISENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            ++T  SGT   P  + +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC  CI+  + + G  CP DN  L E  +FPD+ A REIL L + CP K  GCG  + L  +++H   C++ T          Q+   K+H   E P R                     TCP        C  VL   ++     N   CP A V C +++ GC  K+ R EL  H++  T  H       +R + Q++   S+T  + V       F+  + S  S    D      N   T Q+L   L R+   + +   ++E +N  + E LK+ +       +E EAQ   CNG ++WK+ +F  L K    +   V++S GFYT   GYK+CLR +I L N +    Y+ +FVH M+G+ DS L WPF+G I L+ +       R+  E   ET  E+     AF+RP   RNP GFG+  F+ + AL    F+  ++TL+++ E+ T
Sbjct:   33 EDTVGSGTGNPPGSFAEETQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKGCIVKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNK--GCGMKMELRHLEDHQVQCEFSTVECPQCQGAFQKNHLKEHMTQECPRRQVCCPNCATSMAYEDKELHDQTCPLANVFCEYCNTVLIREQMPNHYAND--CPTAPVPCFYSAFGCPEKMQRNELARHMQEFTQVH-------MRMMAQSFQNISVTATNPVPFLNGLPFEPALFSHVSHAAYDCNPEVENFKETIQQLEGRLVRQDHQIRELIAKMETQNTHVAE-LKRTIRNLEGKITEMEAQ--QCNGIYIWKIENFSGLQKAQEEERPVVIHSPGFYTGKPGYKLCLRLHIQLPNAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLSILDQSEGPERQNQEEVMETKPEL----LAFQRPTIHRNPKGFGYVTFMHLQALKQRTFVK-DDTLLVRCEVLT 522          

BLAST of EMLSAG00000000227 vs. nr
Match: gi|1080060977|ref|XP_018572458.1| (PREDICTED: TNF receptor-associated factor 6-like [Anoplophora glabripennis])

HSP 1 Score: 223.402 bits (568), Expect = 6.756e-65
Identity = 143/426 (33.57%), Postives = 207/426 (48.59%), Query Frame = 0

Query:   33 RFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRR-ELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETAS-----SSFKEGILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEEYLGVFVHLMRGDNDSALSWPFRGQISLTAVREISENFFETIKEI----PEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAE 448
            RFECPICL   RDP  TSCGHRFC +CI +WL     CP DN  L + DIFPD+   REI Q    CP    GC   +   DV+ HL  C+Y       K  E P                                   V C F   GC  K     ELQ H++     H+++L     K+  N +  S  + S++ + A+     +  +   LS+D +L          Q L+K L+ +++ LEQK  + +I    + E++     S  +   RYCNG ++W   DF      MR + H + YS GFYTS  GYK+C+R N++  +   Y+ + +H+M+ ++D+AL WPF G++S+T V       F +IKE     PE  +AF+RP+ + NP GFG+TEF  +  L    FI   N L++K +
Sbjct:   32 RFECPICLAWLRDPVLTSCGHRFCRTCIHSWLERESACPVDNIKLKKDDIFPDNFTRREISQQRTKCPNIIRGCLVELSPLDVEAHLMICEY-------KAPELPENEK-------------------------------VRCSFVDVGCDEKFEDELELQRHLEQFVQKHLTMLSQAYTKMMVNNNVAS--SSSAIAQQANFWDPPAKNESPTLSQDENL----------QGLLKALYEKIIFLEQKTREQDIIIANMSEQISSYNLSVMKLHQRYCNGCYLWYFNDFKSKVNAMRENPHIMHYSAGFYTSANGYKLCVRLNLSPKDN-NYIAILIHVMKTEHDNALDWPFSGRLSITIVHPTQP--FRSIKETMMTRPE-LEAFKRPVLDLNPKGFGYTEFALLEELIEQDFI-YNNQLLIKVQ 402          

BLAST of EMLSAG00000000227 vs. nr
Match: gi|699685679|ref|XP_009903862.1| (PREDICTED: LOW QUALITY PROTEIN: TNF receptor-associated factor 6 [Picoides pubescens])

HSP 1 Score: 227.254 bits (578), Expect = 7.995e-65
Identity = 162/507 (31.95%), Postives = 242/507 (47.73%), Query Frame = 0

Query:    2 DKETGRSGTKGIPKHYFQ--GGYDYDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNE-GRTCPDDNCSLGEGDIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQE-------------RSSKKHENPSRSS----------------DSNTCP-------SCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWDKLRKIQQNYDTPSLTTQSSVDETASSSFKEGILSRDS----DLRLSGSNLHST-QKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVM------CSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE---YLGVFVHLMRGDNDSALSWPFRGQISLTAVREIS----ENFFETIKEIPEGSKAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILKAELFT 451
            D   G SGT   P    +   GYD +F   L  ++ECPICL+  R+  QT CGHRFC  CI+  + + G  CP DN  L E  +FPD+ A REIL LM+ CP K  GCG  + L  +++H   C++ T E             +  +  E P R                     TCP        C  VL   ++     N   CP A V C +++ GC  K+ R EL  H++  T  H       +R + Q++   S+T  + V       F+  + S  S    +      N   T Q+L   L R+   L +   ++E +N  + E LK+ +       +E EAQ   CNG ++WK+ +F  L K    +   V++S GFYT   GYK+CLR +I L + +    ++ +FVH M+G+ DS L WPF+G I L+ + +      +N  E ++  PE   AF+RP   RNP GFG+  F+ +  L    FI  ++TL+++ E+ T
Sbjct:   32 DDSVGSSGTANPPSSLTEETQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKGCIVKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCPNK--GCGVKMELRHLEDHQLQCEFSTVECPQCQGTFQKNHLKEHRTQECPRRQVCCPNCATSMAYEDKELHDQTCPLANVFCEYCNTVLIREQMPNHYDND--CPTAPVPCFYSAFGCPEKMQRNELARHMQEFTQVH-------MRMMAQSFQNISVTAANPVPFINGLPFEPTLFSHASHAPYECNPDVENFKETIQQLEGRLVRQDHQLRELIAKMETQNAHVAE-LKRTIRSLEGKVAELEAQ--QCNGIYIWKIENFSGLQKAQEEEKPVVMHSPGFYTGKPGYKLCLRLHIQLPSAQRCANFISLFVHTMQGEYDSHLPWPFQGTIRLSILDQSEGPERQNHEEVMEAKPE-LLAFQRPTIHRNPKGFGYVTFMHLQTLKQRTFIK-DDTLLVRCEVLT 522          

BLAST of EMLSAG00000000227 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold431_size173393-snap-gene-0.29 (protein:Tk07312 transcript:maker-scaffold431_size173393-snap-gene-0.29-mRNA-1 annotation:"hypothetical protein L798_07735 partial")

HSP 1 Score: 74.7146 bits (182), Expect = 1.384e-14
Identity = 99/412 (24.03%), Postives = 170/412 (41.26%), Query Frame = 0

Query:   81 DIFPDSMAHREILQLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSN-----------TCPSC-----GNVLDDHEITQG--------------QGNRL------VCPYAIVACPFTSAGCAIKIMRRELQDHIKSQTSHHVSLLWD-KLRKIQQNYDTPSLTTQSSVDETASSSFKE-GILSRDSDLRLSGSNLHSTQKLIKDLFRRVVSLEQKNCQLEIRNEMLQEKLKKVMCSEFEAQGRYCNGKFVWKLLDFPELHKKMRTDCHFVVYSKGFYTSVFGYKVCLRSNINLVNGEE--YLGVFVHLMRGDNDSALSWPFRGQISLTAV-----REISENFFETIKEIPEGS-KAFERPISERNPTGFGFTEFIRVNALYSGGFINTENTLILK 446
            ++  D++  ++I+Q MI C  KD GC     L+ ++ HL +C+       ++     +R    +            C  C     G  ++DH+   G              Q NRL       C   +V+C +    C+       LQ+H K      V       L  + +      L  +    +  +  F+E G   R S   L   NL STQ  + DL   +V  +Q +         L  +L++   S         NG  +WK+ DF    ++ ++     + S  FYTS  GYK+     +N   G E  ++ V++ ++ G+ DS L WPF+  +S + +     R+ + N  E+   IP+ S   F+RP  E +  GFGF +F+  + +    +I  E++L +K
Sbjct:  121 NVESDALTKQKIMQSMIYCIHKDEGCKWSGQLNKLKGHLNTCQKDAVGCLNQCGAKIARMMMEDHMLYTCNMRLVGCQYCRKDFSGATIEDHQTQCGFEPVHCENKCGQKIQRNRLKAHQVNTCSKRLVSCQY----CSRHFTADTLQNHHKKCHMFPVPCPNRCSLGSLAREDVERHLAGECEGAQLQACQFREAGCGFRGSKSSLEAHNLESTQAHL-DLMCHLVHKQQSHI------SQLSGQLERAHTSY--------NGVLLWKIKDFKAKIEEAKSSEGLELVSMPFYTSQCGYKLQASLFLNGNGGGESSHMSVYIKILPGEFDSILKWPFKHTVSFSLLDQNEDRKQACNIVESF--IPDPSWPNFQRPSREPDQLGFGFPKFVPHDMMEDRDYIK-EDSLYIK 510          

BLAST of EMLSAG00000000227 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold446_size168061-snap-gene-0.26 (protein:Tk08584 transcript:maker-scaffold446_size168061-snap-gene-0.26-mRNA-1 annotation:"hypothetical protein LOTGIDRAFT_103997")

HSP 1 Score: 58.151 bits (139), Expect = 3.139e-9
Identity = 60/238 (25.21%), Postives = 91/238 (38.24%), Query Frame = 0

Query:   24 YDFVQQLPKRFECPICLLCQRDPHQTSCGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREIL-QLMITCPQKDHGCGAVIHLSDVZNHLRSCKYITQERSSKKHENPSRSSDSNTCPSCGNVLDDHEITQGQGNRLVCPYAIVACPFTSAGCAIKIMRRELQDH-----IKSQTSHHVSLLWDKLRKIQQNYDTPSL--TTQSSVDE-----TASSSFKEGILSRDSDLR 248
            Y++  ++ +   C ICL     P  T C H FC+ CI+ +L     CP D   L E  + P ++  + +L +L +TCP  D                 SC+           E  SR S           L DH        R  C   +VAC F SAGC  +   + +  H      K Q    + +L  ++  I+     PSL  T     D           +++G+L++D  LR
Sbjct:   55 YNYSDEVDEDLMCQICLQPFVQPVDTPCAHTFCHVCILNYLKVNPLCPLDRKPLTEMTVNPTNLVLKRLLDKLEVTCPNSD-----------------SCQ-----------EGVSRGS-----------LSDHL-------RYRCLGTLVACQFASAGCDYRGPTKSMNKHQNECQFKKQGITRLPILEGEVSHIEIPRTRPSLGMTIVGGADTPLRCVVVQEIYQDGLLAQDGRLR 246          

BLAST of EMLSAG00000000227 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold1087_size63509-snap-gene-0.18 (protein:Tk11015 transcript:maker-scaffold1087_size63509-snap-gene-0.18-mRNA-1 annotation:"e3 ubiquitin-protein ligase nrdp1")

HSP 1 Score: 55.0694 bits (131), Expect = 1.760e-8
Identity = 35/115 (30.43%), Postives = 51/115 (44.35%), Query Frame = 0

Query:   13 IPKHYFQGGYDYDFVQ-QLPKRFECPICLLCQRDPHQTS-CGHRFCYSCIITWLNEGRTCPDDNCSLGEGDIFPDSMAHREIL-QLMITCPQKDHGCGAVIHLSDVZNHLRSCKY 124
            +P      G D + +  ++ +   CPIC     DP Q   C H FC  CI  W+    TCP D   +    + P     R +L +L I+C     GC A I L  +  HL+ C++
Sbjct:    1 LPDVALGMGLDVNRIHGEVDEELVCPICSSVLEDPVQAPDCEHAFCRDCISQWVRRQPTCPVDRQPVTAAQLKPVPRILRNMLARLEISCENAGFGCPAKIKLDLLGCHLKECQH 115