EMLSAG00000004269, EMLSAG00000004269-687035 (gene) Lepeophtheirus salmonis

Overview
NameEMLSAG00000004269
Unique NameEMLSAG00000004269-687035
Typegene
OrganismLepeophtheirus salmonis (salmon louse)
Associated RNAi Experiments

Nothing found

Homology
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BLAST of EMLSAG00000004269 vs. C. finmarchicus
Match: gi|592802545|gb|GAXK01152023.1| (TSA: Calanus finmarchicus comp927091_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 71.2478 bits (173), Expect = 6.671e-15
Identity = 47/128 (36.72%), Postives = 74/128 (57.81%), Query Frame = 0 
Query:   14 GLMKIFLLCLSIINVCLARAGF--HLVPYIETVDARWLAYVTSGGYXXXXXXXXXXMIFSNEMSMSADILFSLVGTALYLATGTMAXMAYDD--TEDDYRRNEGMAFGGMCIVTGIIYFIHLVLLLKK 137
            GL KIF L L +I   LAR G+  +   +  +VD  WL Y+T GG+++I   +++ ++  + MS   D LFS+VG  LY+A+G+ A + Y+   T     R+ G+A G  CI+TGI+  +    L+ K
Sbjct:   87 GLFKIFELVLIVITFLLARIGYGGNHALFGTSVDEIWLGYLTVGGWLIITPAIIVGIVLEDPMSWKLDSLFSIVGAFLYIASGSCA-IDYNTSLTLAKESRDSGLALGSFCIITGIVLLMDAAFLVTK 467
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BLAST of EMLSAG00000004269 vs. C. finmarchicus
Match: gi|592864015|gb|GAXK01093547.1| (TSA: Calanus finmarchicus comp3269073_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 39.6614 bits (91), Expect = 4.764e-4
Identity = 22/59 (37.29%), Postives = 35/59 (59.32%), Query Frame = 0 
Query:    1 MAFVYCGFLKEPTGLMKIFLLCLSIINVCLARAGF--HLVPYIETVDARWLAYVTSGGY 57
            MA  + G L   +G++KI  L L+I    LAR GF  +  P+  +++  WL Y+T+GG+
Sbjct:   44 MALHFKGLLTT-SGMLKILELILAITTFVLARVGFDGYTAPFGYSLNHTWLGYLTTGGW 217
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BLAST of EMLSAG00000004269 vs. C. finmarchicus
Match: gi|592759298|gb|GAXK01195115.1| (TSA: Calanus finmarchicus comp88665_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 40.4318 bits (93), Expect = 6.259e-4
Identity = 22/65 (33.85%), Postives = 36/65 (55.38%), Query Frame = 0 
Query:   81 LFSLVGTALYLATGTMAXMAYDD------------TEDDYRRN--EGMAFGGMCIVTGIIYFIHL 131
            +++L+G  +++ +G+MA + + D            TE +Y RN    MA G MCI+ G+IY I L
Sbjct:  337 IWNLLGAFMFMGSGSMAIITWKDEQALKTSSSTSLTEMEYHRNISSAMAMGAMCIIIGLIYLIDL 531
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BLAST of EMLSAG00000004269 vs. C. finmarchicus
Match: gi|592768702|gb|GAXK01185866.1| (TSA: Calanus finmarchicus comp44758_c1_seq6 transcribed RNA sequence)

HSP 1 Score: 37.3502 bits (85), Expect = 7.756e-3
Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 0 
Query:   79 DILFSLVGTALYLATGTMAXMAYDDTED-DYR-RNEGMAFGGMCIVTGIIYFIHLVLLLKKLR 139
            ++L +LVG  L L  G++A   +    D +YR   EG+A G + I+ GI++ I  +L L+ ++
Sbjct:  459 EVLINLVGAILLLTAGSLAISYHSPRYDRNYRDSQEGLALGVIAIIAGIVFAIDFLLSLRHMK 647
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BLAST of EMLSAG00000004269 vs. C. finmarchicus
Match: gi|592768703|gb|GAXK01185865.1| (TSA: Calanus finmarchicus comp44758_c1_seq5 transcribed RNA sequence)

HSP 1 Score: 37.3502 bits (85), Expect = 7.798e-3
Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 0 
Query:   79 DILFSLVGTALYLATGTMAXMAYDDTED-DYR-RNEGMAFGGMCIVTGIIYFIHLVLLLKKLR 139
            ++L +LVG  L L  G++A   +    D +YR   EG+A G + I+ GI++ I  +L L+ ++
Sbjct:  459 EVLINLVGAILLLTAGSLAISYHSPRYDRNYRDSQEGLALGVIAIIAGIVFAIDFLLSLRHMK 647
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BLAST of EMLSAG00000004269 vs. C. finmarchicus
Match: gi|592768704|gb|GAXK01185864.1| (TSA: Calanus finmarchicus comp44758_c1_seq4 transcribed RNA sequence)

HSP 1 Score: 36.965 bits (84), Expect = 1.128e-2
Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 0 
Query:   79 DILFSLVGTALYLATGTMAXMAYDDTED-DYR-RNEGMAFGGMCIVTGIIYFIHLVLLLKKLR 139
            ++L +LVG  L L  G++A   +    D +YR   EG+A G + I+ GI++ I  +L L+ ++
Sbjct:  598 EVLINLVGAILLLTAGSLAISYHSPRYDRNYRDSQEGLALGVIAIIAGIVFAIDFLLSLRHMK 786
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BLAST of EMLSAG00000004269 vs. C. finmarchicus
Match: gi|592768705|gb|GAXK01185863.1| (TSA: Calanus finmarchicus comp44758_c1_seq3 transcribed RNA sequence)

HSP 1 Score: 36.965 bits (84), Expect = 1.132e-2
Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 0 
Query:   79 DILFSLVGTALYLATGTMAXMAYDDTED-DYR-RNEGMAFGGMCIVTGIIYFIHLVLLLKKLR 139
            ++L +LVG  L L  G++A   +    D +YR   EG+A G + I+ GI++ I  +L L+ ++
Sbjct:  598 EVLINLVGAILLLTAGSLAISYHSPRYDRNYRDSQEGLALGVIAIIAGIVFAIDFLLSLRHMK 786
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BLAST of EMLSAG00000004269 vs. C. finmarchicus
Match: gi|592768706|gb|GAXK01185862.1| (TSA: Calanus finmarchicus comp44758_c1_seq2 transcribed RNA sequence)

HSP 1 Score: 36.965 bits (84), Expect = 1.381e-2
Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 0 
Query:   79 DILFSLVGTALYLATGTMAXMAYDDTED-DYR-RNEGMAFGGMCIVTGIIYFIHLVLLLKKLR 139
            ++L +LVG  L L  G++A   +    D +YR   EG+A G + I+ GI++ I  +L L+ ++
Sbjct:  635 EVLINLVGAILLLTAGSLAISYHSPRYDRNYRDSQEGLALGVIAIIAGIVFAIDFLLSLRHMK 823
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BLAST of EMLSAG00000004269 vs. C. finmarchicus
Match: gi|592768707|gb|GAXK01185861.1| (TSA: Calanus finmarchicus comp44758_c1_seq1 transcribed RNA sequence)

HSP 1 Score: 36.965 bits (84), Expect = 1.386e-2
Identity = 21/63 (33.33%), Postives = 37/63 (58.73%), Query Frame = 0 
Query:   79 DILFSLVGTALYLATGTMAXMAYDDTED-DYR-RNEGMAFGGMCIVTGIIYFIHLVLLLKKLR 139
            ++L +LVG  L L  G++A   +    D +YR   EG+A G + I+ GI++ I  +L L+ ++
Sbjct:  635 EVLINLVGAILLLTAGSLAISYHSPRYDRNYRDSQEGLALGVIAIIAGIVFAIDFLLSLRHMK 823
[Close]
BLAST of EMLSAG00000004269 vs. C. finmarchicus
Match: gi|592764203|gb|GAXK01190365.1| (TSA: Calanus finmarchicus comp1492330_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 32.3426 bits (72), Expect = 1.905e-1
Identity = 13/35 (37.14%), Postives = 20/35 (57.14%), Query Frame = 0 
Query:   14 GLMKIFLLCLSIINVCLARAGFHLVPYIETVDARW 48
            G++ +F +C+SI++ CL       VP I  V  RW
Sbjct:   39 GVISVFFICVSILSFCLKTHPDMRVPVIRNVTVRW 143
[Close]
BLAST of EMLSAG00000004269 vs. L. salmonis peptides
Match: EMLSAP00000004269 (pep:novel supercontig:LSalAtl2s:LSalAtl2s224:170363:172901:1 gene:EMLSAG00000004269 transcript:EMLSAT00000004269 description:"maker-LSalAtl2s224-snap-gene-2.14")

HSP 1 Score: 291.582 bits (745), Expect = 5.733e-102
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0 
Query:    1 MAFVYCGFLKEPTGLMKIFLLCLSIINVCLARAGFHLVPYIETVDARWLAYVTSGGYVLILSVLVLSMIFSNEMSMSADILFSLVGTALYLATGTMAXMAYDDTEDDYRRNEGMAFGGMCIVTGIIYFIHLVLLLKKLRSNKSTI 145
            MAFVYCGFLKEPTGLMKIFLLCLSIINVCLARAGFHLVPYIETVDARWLAYVTSGGYVLILSVLVLSMIFSNEMSMSADILFSLVGTALYLATGTMAXMAYDDTEDDYRRNEGMAFGGMCIVTGIIYFIHLVLLLKKLRSNKSTI
Sbjct:    1 MAFVYCGFLKEPTGLMKIFLLCLSIINVCLARAGFHLVPYIETVDARWLAYVTSGGYVLILSVLVLSMIFSNEMSMSADILFSLVGTALYLATGTMAXMAYDDTEDDYRRNEGMAFGGMCIVTGIIYFIHLVLLLKKLRSNKSTI 145
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BLAST of EMLSAG00000004269 vs. L. salmonis peptides
Match: EMLSAP00000008414 (pep:novel supercontig:LSalAtl2s:LSalAtl2s514:285134:288837:-1 gene:EMLSAG00000008414 transcript:EMLSAT00000008414 description:"maker-LSalAtl2s514-augustus-gene-2.23")

HSP 1 Score: 54.6842 bits (130), Expect = 1.724e-9
Identity = 32/131 (24.43%), Postives = 68/131 (51.91%), Query Frame = 0 
Query:    9 LKEPTGLMKIFLLCLSIINVCLARAGFHLVPYIETVDARWLAYVTSGGYVLILSVLVLSMIFSNEMSMSADILFSLVGTALYLATGTMAXMAYDDTEDDYRRNEGMAFGGMCIVTGIIYFIHLVLLLKKLR 139
            L+ P+G +K+  + L  I + L R  F       + D +++   T  GY +I+  ++L+ +  + MS+  ++  +L+G+ L++  G +    Y       + N+G+A G +CIV+G ++ I  +  ++  R
Sbjct:  709 LRTPSGFLKLLEIILVFIILILVRFVFEHEYIFGSTDMKFIGIGTIVGYSIIVPCVLLTYMLGSNMSI-LELFINLIGSILFIVIGAIVIQTYSGCRRRGQCNDGLALGSLCIVSGXLFLIDFLFAIRSTR 838
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BLAST of EMLSAG00000004269 vs. nr
Match: gi|936680121|ref|XP_014234970.1| (PREDICTED: uncharacterized protein LOC106657804 [Trichogramma pretiosum])

HSP 1 Score: 54.6842 bits (130), Expect = 6.772e-7
Identity = 39/123 (31.71%), Postives = 64/123 (52.03%), Query Frame = 0 
Query:   15 LMKIFLLCLSIINVCLARAGFHLVPYIETVDARWLAYVTSGGYVLILSVLVLSMIFSNEMSMSADILFSLVGTALYLATGTMAXMAYDDTEDDYRRNE-GMAFGGMCIVTGIIYFIHLVLLLK 136
            L+++ L+C+ I        G H   YI T++   L   T  GY++IL  L    +    ++   D+ FSLVG AL++A+G++        + DY RN+   A G + I+ GII+FI  +L  +
Sbjct:   12 LLELILVCVLI--------GLHYHSYIGTLNDAMLVTGTFCGYLIILVGLFAGAVMGTPVNRRVDLFFSLVGCALFIASGSLII------DHDYARNDKSTAKGSIAIIEGIIFFIDAILTFR 120
[Close]
BLAST of EMLSAG00000004269 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold11_size778918-snap-gene-0.14 (protein:Tk03654 transcript:maker-scaffold11_size778918-snap-gene-0.14-mRNA-1 annotation:"PREDICTED: uncharacterized protein LOC100871751")

HSP 1 Score: 79.337 bits (194), Expect = 5.343e-20
Identity = 46/135 (34.07%), Postives = 75/135 (55.56%), Query Frame = 0 
Query:    9 LKEPTGLMKIFLLCLSIINVCLARAGFHL--VPYIETVDARWLAYVTSGGYVLILSVLVLSMIFSNEMSMSADILFSLVGTALYLATGTMAXMAYDDTEDDYRRNEGMAFGGMCIVTGIIYFIHLVLLLKKLRSN 141
            +K P+G++K   L L+I+ + LAR GF+     Y   VD +WL   T GGY +I   +VL+    + +    +I+FS VG+ L++ATG +        E     + G+A G + I+TGII F+  +LL K +++ 
Sbjct:    8 IKAPSGMIKFLCLALTIVVIILARIGFNEDETSY-RNVDHKWLTITTFGGYCIIFGAMVLTYFLGSGVPDKMEIVFSFVGSCLFIATGAI------HVEHHEGNDTGLAMGSLSIITGIILFVDCLLLAKAMKAR 135
The following BLAST results are available for this feature:
BLAST of EMLSAG00000004269 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000004269 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA)
Total hits: 12
Match NameE-valueIdentityDescription
gi|592802545|gb|GAXK01152023.1|6.671e-1536.72TSA: Calanus finmarchicus comp927091_c0_seq1 trans... [more]
gi|592864015|gb|GAXK01093547.1|4.764e-437.29TSA: Calanus finmarchicus comp3269073_c0_seq1 tran... [more]
gi|592759298|gb|GAXK01195115.1|6.259e-433.85TSA: Calanus finmarchicus comp88665_c0_seq1 transc... [more]
gi|592768702|gb|GAXK01185866.1|7.756e-333.33TSA: Calanus finmarchicus comp44758_c1_seq6 transc... [more]
gi|592768703|gb|GAXK01185865.1|7.798e-333.33TSA: Calanus finmarchicus comp44758_c1_seq5 transc... [more]
gi|592768704|gb|GAXK01185864.1|1.128e-233.33TSA: Calanus finmarchicus comp44758_c1_seq4 transc... [more]
gi|592768705|gb|GAXK01185863.1|1.132e-233.33TSA: Calanus finmarchicus comp44758_c1_seq3 transc... [more]
gi|592768706|gb|GAXK01185862.1|1.381e-233.33TSA: Calanus finmarchicus comp44758_c1_seq2 transc... [more]
gi|592768707|gb|GAXK01185861.1|1.386e-233.33TSA: Calanus finmarchicus comp44758_c1_seq1 transc... [more]
gi|592764203|gb|GAXK01190365.1|1.905e-137.14TSA: Calanus finmarchicus comp1492330_c0_seq1 tran... [more]

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BLAST of EMLSAG00000004269 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self)
Total hits: 2
Match NameE-valueIdentityDescription
EMLSAP000000042695.733e-102100.00pep:novel supercontig:LSalAtl2s:LSalAtl2s224:17036... [more]
EMLSAP000000084141.724e-924.43pep:novel supercontig:LSalAtl2s:LSalAtl2s514:28513... [more]
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BLAST of EMLSAG00000004269 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000004269 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000004269 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017))
Total hits: 1
Match NameE-valueIdentityDescription
gi|936680121|ref|XP_014234970.1|6.772e-731.71PREDICTED: uncharacterized protein LOC106657804 [T... [more]
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BLAST of EMLSAG00000004269 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins)
Total hits: 1
Match NameE-valueIdentityDescription
maker-scaffold11_size778918-snap-gene-0.145.343e-2034.07protein:Tk03654 transcript:maker-scaffold11_size77... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
LSalAtl2s224supercontigLSalAtl2s224:170363..172901 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
ensembl2013-09-26 .965016
Blast vs. GO2014-04-02
TblastN vs C. finmarchicus TSA2014-05-09
Blastp vs. self2014-05-10
Blastp vs. SwissProt2017-02-10
Blastp vs. Selected Arthropods2017-02-20
Blastp vs. NR (2/2017)2017-02-20
Blastp vs. Tigriopus kingsejongensis proteins2018-04-18
Properties
Property NameValue
Logic nameensemblgenomes
Descriptionmaker-LSalAtl2s224-snap-gene-2.14
Biotypeprotein_coding
EvidenceIEA
NoteHypothetical protein
Cross References
External references for this gene
DatabaseAccession
Ensembl Metazoa (gene)EMLSAG00000004269 (primary cross-reference)
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
EMLSAT00000004269EMLSAT00000004269-700116Lepeophtheirus salmonismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at LSalAtl2s224:170363..172901+

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>EMLSAG00000004269-687035 ID=EMLSAG00000004269-687035|Name=EMLSAG00000004269|organism=Lepeophtheirus salmonis|type=gene|length=2539bp|location=Sequence derived from alignment at LSalAtl2s224:170363..172901+ (Lepeophtheirus salmonis)
AAAAAAATAAAGAAATATTTTATATTCTACTACTACACGCTCTAAGTTTC
AGTCAAGTATTCCGTTTGAGTCAGTTTAGACACGTGAAGCAGTTCCTGAA
CTTTTTTTTGCTAGTTGCGAGTTTGTTTCCTGATCGTTCATCACTTTGTG
CTCCCTTTTTATAATAATCTATTATCCCTAAAAGAAAATGGCTTTTGTTT
ACTGTGGATTCCTTAAAGAGCCTACGGGTCTCATGAAGATTTTCTTACTC
GTAAGTAGAGAACGAATAATAATCTTGTGAAGTGGGCGCTAAGGATCAAT
GGATAATATCTGCAAAAAGTGGGTGTCGCACGCCTGAATGTTATGACTGT
AATAATGAGTTAAGTGAGAGGAAATCTATTCGTAGATTAATGTTAGGACG
GAGTTTGTGATCAGATGGGGTTTTATTTATATACATTTCTTATTTAATGG
GGGTTGCAGAATAATTAATTATTGCCAGTTAGACGGATCCGAGATCTGTA
CATGGTGTAGTAGTAGTGGATATTACGAATATGACGCTTGCAGGTACAGA
GAGGGTGAGTGGAGGYCGCAGMGCTTTGCTGTTGTGTGTCGTAGNTGAGT
TGGACWAAGAGGAGCAGCGGCTTTGCGTATTAATAGTTACAACAAATMTT
ATASCTGCCATTTTTTACTACTACAACTAATAATAAGTAGTATAATATAC
CTCCTCCTCCTTGTGTAATTTGACATGGAACTTTCATAATAAAAATAGCG
TCTATATAAATGCTATGTTGTACGTACGTACGACAAGGGAGGAAGAAAAG
AGAGCGGGAAATATAGGAGAAATGAAACTATGATGATATGCGACCTTGAT
TTTAGCACAGAAATGTTTTTTCACAACTAAAGCATTTGTATTTTAGAGGA
AAAGAGGAACGCTGCGACAGAGGGGGGGGGGGCGTGCAAAAAAAAGAAAA
AAAGATCAAATATTCTTACTATTAGGACTAAAAAATGAGAAAAAATCCTG
ATTTTTCCTACATTTCAGTGCCTCAGCATTATCAACGTATGCCTAGCCCG
TGCTGGATTTCATTTGGTTCCTTACATTGAGACTGTAGATGCTCGATGGC
TCGCTTATGTAACATCTGGTGGATATGTTCTCATTCTCTCAGTCCTTGTT
CTCTCCATGATCTTCTCGAATGAGATGTCCATGAGTGCGGTAAGATTGAA
TGCGCCATCTCTTAAACAACATTTACTATTCTTTTTATTTATTTATTTTC
AGGATATTCTCTTTAGTTTGGTTGGAACTGCCCTTTATCTTGCCACAGGA
ACCATGGCTGMTATGGCCTATGATGATACCGAGGATGATTACCGACGAAA
CGAGGGAATGGCTTTTGGAGGGATGTGCATCGTGACTGGGATCATTTACT
TTATTCATTTAGTACTACTATTAAAGAAATTGAGGAGTAACAAATCAACA
ATCTAAGTCGTCATTTGTTATTAAATCAAATCTTCTACATTGATTATAAT
ATCTTTAAATTGTAATTTTCTCTTTCTGTGTATCAAAAAACCCCTGAAAC
TGTTTCTTTATGAAAGAAAGGGGACCAAAGCCTTATAAATAAACTATCCC
GCCCAAATCATAGCTTTTAATTCAATTTTTCCCCAAATTTAAATTTTAAT
TTATATATCAATATAAATATATATATATATTTCATTATTTAAATGCTTTT
TTTTTCATTATCTTTATTAGAGCCAAAGTTTGGTGTATTTCTGTGTTCAG
CTTATTCAACCCCATTTCGATGGGAGACAAACAATATTTATTCACTTTAT
GGCATTAAGAGATATGTTGATTTTTTTTTAAATTCAAATCTAATAAAAGT
TGCACTGCAATTAGATTGCTTTATTTTACAAATGAAGGCGTTGTAACGCC
CCCCCCCCCCCCTCCCTACCTCTTATTATAAATAAGCTCATCAGCAAACT
CCTTCCCACCCAATAATGTAGTATCCTCTATAATAATATTACCCTCCATT
CTTTTATATATTCATGAAAGTAATTTGGTTTATTATGTGTTACCCCCGGG
CTATTTTTTATATACATTGTAATACGCTTCACTAACTTAAGTATTAGACT
TTATTTTCATGTTATAGATACTATTATGTGGAAGGTCGCCCAAAAAAATA
ATAAACGACAAAAAAATACGCAAAGACATGATCACGCAACTATTGACATT
TCAAAAAAGAATCCGTCTTTAACTCTTATATTCAGTGAGTCATTTATTTT
TCATTAAGGACAAGAAACTTTCTTATGTTGAATAAATATTGTTGTCTATT
TATCATCGAAGGTGTGATAATAATGATTGTTAAAATTTCTGCTAACAAAT
AATGTGCTAAAGTAAACCTTTCGTAGGCAGGATTGTTATCTTGAAGTATT
TGAATATCTAATGTATAACTTTTTAAGGGAGGAAGAGCGATGCATATTAG
TGTTGCCTCAACTCGACCAATTATCCACTCTGCTCAGTTTGAATCGAGTA
GATTTTCAAATTCAAAAAAGCCAGTCACCTTGATTTTAA
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