BLAST of EMLSAG00000007474 vs. GO
Match: - (symbol:unc-13 "unc-13" species:7227 "Drosophila melanogaster" [GO:0019992 "diacylglycerol binding" evidence=ISS] [GO:0016079 "synaptic vesicle exocytosis" evidence=ISS;IMP] [GO:0007268 "synaptic transmission" evidence=NAS;IMP] [GO:0005516 "calmodulin binding" evidence=TAS] [GO:0016082 "synaptic vesicle priming" evidence=NAS] [GO:0007269 "neurotransmitter secretion" evidence=NAS] [GO:0008021 "synaptic vesicle" evidence=ISS;NAS] [GO:0043195 "terminal bouton" evidence=IDA] [GO:0045211 "postsynaptic membrane" evidence=IDA] [GO:0035556 "intracellular signal transduction" evidence=IEA] InterPro:IPR000008 InterPro:IPR002219 InterPro:IPR027080 Pfam:PF00130 Pfam:PF00168 PRINTS:PR00360 PROSITE:PS00479 PROSITE:PS50004 PROSITE:PS50081 SMART:SM00109 SMART:SM00239 GO:GO:0035556 GO:GO:0046872 GO:GO:0045211 GO:GO:0008021 GO:GO:0043195 SUPFAM:SSF49562 GO:GO:0005516 EMBL:AE014135 InterPro:IPR014770 InterPro:IPR014772 InterPro:IPR019558 Pfam:PF10540 PROSITE:PS51258 PROSITE:PS51259 GO:GO:0019992 GO:GO:0016082 InterPro:IPR010439 Pfam:PF06292 KO:K15293 OrthoDB:EOG76738V PANTHER:PTHR10480 GeneTree:ENSGT00730000110590 CTD:43841 RefSeq:NP_001245427.1 UniGene:Dm.3358 ProteinModelPortal:H9XVP2 SMR:H9XVP2 EnsemblMetazoa:FBtr0308806 GeneID:43841 KEGG:dme:Dmel_CG2999 FlyBase:FBgn0025726 PhylomeDB:H9XVP2 GenomeRNAi:43841 Uniprot:H9XVP2)

HSP 1 Score: 925.235 bits (2390), Expect = 0.000e+0
Identity = 447/681 (65.64%), Postives = 534/681 (78.41%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGG-----GQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQG 1030
            VDPNEAKR  +D  P+LD ++FWHKLIALI SVI+EDKNSYG VLNQFPQELN+GQLSA+++W LFAVD KYALEEHEQ RLCKSS Y+N HFRVKWL+  YV  VPPYKG VP+YPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQK++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYM+RFAKTIVKVL AYA+I+K +F  H+KDE  AC+LMNNIQQ R+QLEK + +MGG +LEEDAA++L +LQ  LN  LD+LA  FA+S   R+  SV  +G +L  +KGG         A  ++ V   AD +L+PLMDLLDGSL++YA+ C+K+VLKRLLKELWKIVM+ LEK IVLPP TDK+   K+L  + KN+A    K+ED+   FKS   GKQDVKS+ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK +++ KS EL+SL++ALSLYTQ TD LIKT++SSQ H+    + E SVGE+SVQ+DLF+HPGTGEHKV VKV+                     LIGPHL +KKR   TKSK+ NWSPKYNE FSF IGNE+Q+  +ELHICVKDYCF RDDRL+GV+V+ LKDI ++GS ACWL L RRI+MDETGWTILRILSQR NDEVAKEFVKLKSE+R E P  G
Sbjct: 2508 VDPNEAKRAPDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFPQELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVPDYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEHMKDERIACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLPPMTDKTMMFKHLTDNAKNLASNA-KIEDMGRLFKSHMAGKQDVKSA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFISSQVHEVDLENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIPSGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCFARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEIRQE-PTMG 3185          

HSP 2 Score: 550.436 bits (1417), Expect = 1.378e-166
Identity = 253/313 (80.83%), Postives = 284/313 (90.73%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+DKAN+II AMK+RMK+RERE+PEIFELIR +FS+   +H   M   +Q+VLDGTSKWS KIA TVICAQGLIAKDK+GTSDPYVTVQV K KKRT+T+PQELNP WNEKF+FECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G V LP  +G+DAWK+YFDE  +EIVDEF++RYG+E+I+QAMTHFHCLS+KYLCPGVPA MSTLLANINA+YAHTTA++ V
Sbjct: 2088 LQRAAEKSSKHGAEDKANSIITAMKDRMKQREREKPEIFELIRAVFSVEEKSHAGHMKAVKQSVLDGTSKWSAKIAITVICAQGLIAKDKSGTSDPYVTVQVSKVKKRTRTMPQELNPVWNEKFHFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHFHCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAV 2400          

HSP 3 Score: 72.0182 bits (175), Expect = 3.429e-11
Identity = 41/85 (48.24%), Postives = 46/85 (54.12%), Query Frame = 0

Query:   13 GNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSE----------------------------LKLHVYKKXLQALIYPISSAA 69
            G R+N    DNPF+ +IDSMP + RPRRKSIPLVSE                            LK+HVYKK LQALIYPISS  
Sbjct: 1953 GLRTNGHPGDNPFYSNIDSMP-DIRPRRKSIPLVSELVLKTMAATKRNAGLTSAVPRATLNDEDLKMHVYKKALQALIYPISSTT 2036          

BLAST of EMLSAG00000007474 vs. GO
Match: - (symbol:UNC13B "Uncharacterized protein" species:9823 "Sus scrofa" [GO:0007528 "neuromuscular junction development" evidence=IEA] [GO:0016081 "synaptic vesicle docking involved in exocytosis" evidence=IEA] [GO:0016082 "synaptic vesicle priming" evidence=IEA] [GO:0019992 "diacylglycerol binding" evidence=IEA] [GO:0031594 "neuromuscular junction" evidence=IEA] [GO:0035249 "synaptic transmission, glutamatergic" evidence=IEA] [GO:0035556 "intracellular signal transduction" evidence=IEA] [GO:0046872 "metal ion binding" evidence=IEA] [GO:0048172 "regulation of short-term neuronal synaptic plasticity" evidence=IEA] [GO:0060384 "innervation" evidence=IEA] InterPro:IPR000008 InterPro:IPR002219 InterPro:IPR027080 Pfam:PF00130 Pfam:PF00168 PRINTS:PR00360 PROSITE:PS00479 PROSITE:PS50004 PROSITE:PS50081 SMART:SM00109 SMART:SM00239 GO:GO:0035556 GO:GO:0046872 GO:GO:0007528 GO:GO:0031594 GO:GO:0035249 SUPFAM:SSF49562 InterPro:IPR014770 InterPro:IPR014772 InterPro:IPR019558 Pfam:PF10540 PROSITE:PS51258 PROSITE:PS51259 GO:GO:0048172 GO:GO:0016081 GO:GO:0019992 GO:GO:0016082 InterPro:IPR010439 Pfam:PF06292 GO:GO:0060384 OrthoDB:EOG76738V TreeFam:TF312844 PANTHER:PTHR10480 GeneTree:ENSGT00730000110590 OMA:FQGSPDK EMBL:CU468587 Ensembl:ENSSSCT00000005843 Uniprot:F1SIH2)

HSP 1 Score: 753.051 bits (1943), Expect = 0.000e+0
Identity = 367/657 (55.86%), Postives = 471/657 (71.69%), Query Frame = 0

Query:  387 EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            E+ GP + +L+FW KLI LI S+IEEDKNSY  VLNQFPQELNVG++SA  +W LFA D KYALEEHE+ RLCKS+ Y+N HF+VKWLH  YV  +P  +GQVPEYPAWFE FV+QWL+EN+DVSLEFL  A  RDKKD FQ+ +EH+ FS SVVDVFTQL Q FE+++KLEC DP I   YM+RFAKTI KVL  YA+IL  +F  +   E   C+LMNN+QQ R+QLEK + AMGG+EL+ +AA  L +LQ  LN VLDEL+ +F  SF  R+D  V  M  +L +V+G G     +++ V   AD +L+PLMD LDG+L+++A +C+K+VLKR+LKELW++VM ++E+ IVLPP TD+                        TG    F   +++ S +  +   + +E  R+L+PKQC++L+ AL+ IKQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTD LIKT+V SQT Q S   +   VGEVS+QVDLFTHPGTGEHKVTVKV+                    ++GPH SDKKR   TKSK+ NW+PKYNE F F++GNE+   +YEL ICVKDYCF R+DR++G++V+ L+D+  +GSCACW  LGR+I MDETG TILRILSQR+NDEVA+EFVKLKSE R
Sbjct:  571 EEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMEYADILSKNFPAYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNTRASVTQDADSVLRPLMDFLDGNLTLFAAVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQ------------------------TGTQLIFTAAKEL-SHLSKLKDHMVREETRNLTPKQCAILDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGSGVDD--PVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESR 1200          

HSP 2 Score: 489.96 bits (1260), Expect = 5.474e-152
Identity = 233/321 (72.59%), Postives = 274/321 (85.36%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYF--ECHNSSD-RIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNG-GNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            +  AAEKSSKHGA+D+   II+AMK+RMK RER +PEIFE+IR++FS+S   H++ M   +Q+VLDGTSKWS KI  TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W EKF+   E H S+   IKVRVWDED+D+KS+++Q+  RESDD  GQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRLQI+VEIKGEEKVAPYHVQYTCLHENLFHYL +  G G V +PEARG+DAWK+YFDE  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV  ++
Sbjct:  140 LQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFSVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHLMMEVHPSTFLDIKVRVWDEDDDIKSRVKQRLKRESDDSFGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQINVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVQIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASD 460          

BLAST of EMLSAG00000007474 vs. GO
Match: - (symbol:F1NY18 "Uncharacterized protein" species:9031 "Gallus gallus" [GO:0007528 "neuromuscular junction development" evidence=IEA] [GO:0016081 "synaptic vesicle docking involved in exocytosis" evidence=IEA] [GO:0016082 "synaptic vesicle priming" evidence=IEA] [GO:0019992 "diacylglycerol binding" evidence=IEA] [GO:0031594 "neuromuscular junction" evidence=IEA] [GO:0035249 "synaptic transmission, glutamatergic" evidence=IEA] [GO:0035556 "intracellular signal transduction" evidence=IEA] [GO:0046872 "metal ion binding" evidence=IEA] [GO:0048172 "regulation of short-term neuronal synaptic plasticity" evidence=IEA] [GO:0060384 "innervation" evidence=IEA] InterPro:IPR000008 InterPro:IPR002219 InterPro:IPR027080 Pfam:PF00130 Pfam:PF00168 PRINTS:PR00360 PROSITE:PS00479 PROSITE:PS50004 PROSITE:PS50081 SMART:SM00109 SMART:SM00239 GO:GO:0035556 GO:GO:0046872 GO:GO:0007268 SUPFAM:SSF49562 InterPro:IPR014770 InterPro:IPR014772 InterPro:IPR019558 Pfam:PF10540 PROSITE:PS51258 PROSITE:PS51259 GO:GO:0019992 InterPro:IPR010439 Pfam:PF06292 OrthoDB:EOG76738V TreeFam:TF312844 PANTHER:PTHR10480 GeneTree:ENSGT00730000110590 EMBL:AC190132 EMBL:AC191647 Ensembl:ENSGALT00000003394 Uniprot:F1NY18)

HSP 1 Score: 761.911 bits (1966), Expect = 0.000e+0
Identity = 384/718 (53.48%), Postives = 489/718 (68.11%), Query Frame = 0

Query:  343 CLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQ-----------------DSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            CL+S Y           +  N +  Y+    T   DP+      E+ GP + +L+FW KLI LI S+IEEDKNSY PVLNQFPQEL VG++SA  +W LFA D KYA+EEHE+ RLCKS+ Y+N HF+VKWL+  YV  +P +KG+VPEYPAWFE FVMQWL+EN+DVSLEFLH A  RDKKD FQ+ +EH+ FS SVVDVFTQL Q FE+++KLEC DP I   Y +RFAKTI KVL  YA+IL   F ++   E   C+LMNNIQQ R+QLEK + AMGG+EL+ +A+  L +LQ  LN VLDEL+ +F  SF  R+D  V  M  +L +VKG G   A +++ V   AD +L+PLMD LDG+L+++A +C+K+VLKR+LKELW++VM ++EK IVLPP TD +                    + +    K  G    +K  ++       +E  RSL+PKQC+VL+ AL+ IKQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTD LIKT+V +QT Q                 D +S  +  VGEVS+Q+DL+THPGTGEHKVTVKV+                    +IGPH SDKKR   TKSK+ NW+PKYNE F FI+GNED   +YEL +CVKDYCF R+DR++G++V+QL+DI D+GSCACW  LGRRI MDETG T+LRILSQRTNDEVAKEFVKLKSE R
Sbjct:  925 CLNSTY---------EYIFNNCHELYSREYQT---DPDLP---PEEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLNQFPQELAVGKISAEVMWSLFAQDMKYAMEEHEKHRLCKSADYMNLHFKVKWLYNEYVRDLPAFKGKVPEYPAWFEQFVMQWLDENEDVSLEFLHGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIKKLECPDPVIVAHYNRRFAKTIGKVLMQYADILSKSFQSYCSKEKLPCILMNNIQQLRVQLEKMFEAMGGKELDVEASDHLKELQVKLNNVLDELSAVFGNSFQTRIDECVKQMSDILCQVKGTGNVAANARNTVAQDADNVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMEKLIVLPPLTDHTG------------------TQLIFSAAKELGHLSKLKDHMV-------REETRSLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVQTQTAQVHDGKGIRFTANEDVLPDKASGVDDPVGEVSIQIDLYTHPGTGEHKVTVKVVAANDLKWQTSGMFRPFVEITMIGPHQSDKKRKFTTKSKSNNWAPKYNETFHFILGNEDGPDAYELQVCVKDYCFAREDRVLGIAVMQLRDIADKGSCACWCPLGRRIHMDETGLTVLRILSQRTNDEVAKEFVKLKSESR 1602          

HSP 2 Score: 508.834 bits (1309), Expect = 8.119e-156
Identity = 236/314 (75.16%), Postives = 275/314 (87.58%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNG-GNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+D+    I+AMK+RMK RER +PEIF+LIR++F  S   H + M   +Q+VLDGTSKWS KI  TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W EKFYFECHNSSDRIKVRVWDED+D+KS+++Q+  RESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRLQI+VEIKGEEKVAPYH+QYTCLHENLFH+L +  G G V +P+A+G+DAWK+YFDE  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  526 LQRAAEKSSKHGAEDRTQNFIMAMKDRMKIRERNKPEIFDLIRDVFCESKLTHAQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFYFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQINVEIKGEEKVAPYHIQYTCLHENLFHHLTDVQGNGVVKIPDAKGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 839          

BLAST of EMLSAG00000007474 vs. GO
Match: - (symbol:UNC13B "Protein unc-13 homolog B" species:9606 "Homo sapiens" [GO:0004871 "signal transducer activity" evidence=TAS] [GO:0004872 "receptor activity" evidence=TAS] [GO:0005515 "protein binding" evidence=IPI] [GO:0005737 "cytoplasm" evidence=TAS] [GO:0005794 "Golgi apparatus" evidence=IDA] [GO:0005829 "cytosol" evidence=IDA] [GO:0005886 "plasma membrane" evidence=IEA] [GO:0006915 "apoptotic process" evidence=TAS] [GO:0007165 "signal transduction" evidence=TAS] [GO:0007528 "neuromuscular junction development" evidence=IEA] [GO:0007588 "excretion" evidence=TAS] [GO:0016081 "synaptic vesicle docking involved in exocytosis" evidence=IEA] [GO:0016082 "synaptic vesicle priming" evidence=IEA] [GO:0019992 "diacylglycerol binding" evidence=IEA] [GO:0030054 "cell junction" evidence=IEA] [GO:0031594 "neuromuscular junction" evidence=IEA] [GO:0035249 "synaptic transmission, glutamatergic" evidence=IEA] [GO:0035556 "intracellular signal transduction" evidence=IEA] [GO:0046872 "metal ion binding" evidence=IEA] [GO:0048172 "regulation of short-term neuronal synaptic plasticity" evidence=IEA] [GO:0060384 "innervation" evidence=IEA] InterPro:IPR000008 InterPro:IPR002219 InterPro:IPR027080 Pfam:PF00130 Pfam:PF00168 PRINTS:PR00360 PROSITE:PS00479 PROSITE:PS50004 PROSITE:PS50081 SMART:SM00109 SMART:SM00239 GO:GO:0005829 GO:GO:0005886 GO:GO:0005794 GO:GO:0006915 GO:GO:0035556 GO:GO:0046872 GO:GO:0030054 GO:GO:0007528 GO:GO:0004872 GO:GO:0031594 GO:GO:0035249 GO:GO:0007588 SUPFAM:SSF49562 GO:GO:0004871 EMBL:CH471071 InterPro:IPR014770 InterPro:IPR014772 InterPro:IPR019558 Pfam:PF10540 PROSITE:PS51258 PROSITE:PS51259 GO:GO:0048172 GO:GO:0016081 GO:GO:0019992 GO:GO:0016082 InterPro:IPR010439 Pfam:PF06292 EMBL:AL353795 GO:GO:0060384 eggNOG:NOG245727 HOVERGEN:HBG057340 KO:K15293 TreeFam:TF312844 PANTHER:PTHR10480 EMBL:AF020202 EMBL:AL160274 EMBL:AL354669 RefSeq:NP_006368.3 UniGene:Hs.493791 ProteinModelPortal:O14795 SMR:O14795 BioGrid:115759 IntAct:O14795 MINT:MINT-1395851 STRING:9606.ENSP00000367756 PhosphoSite:O14795 PaxDb:O14795 PRIDE:O14795 Ensembl:ENST00000378495 GeneID:10497 KEGG:hsa:10497 UCSC:uc003zwq.3 CTD:10497 GeneCards:GC09P035117 H-InvDB:HIX0034747 HGNC:HGNC:12566 HPA:HPA024493 MIM:605836 neXtProt:NX_O14795 PharmGKB:PA37203 PhylomeDB:O14795 GeneWiki:UNC13B GenomeRNAi:10497 NextBio:39832 PRO:PR:O14795 ArrayExpress:O14795 Bgee:O14795 CleanEx:HS_UNC13B Genevestigator:O14795 Uniprot:O14795)

HSP 1 Score: 755.362 bits (1949), Expect = 0.000e+0
Identity = 369/657 (56.16%), Postives = 470/657 (71.54%), Query Frame = 0

Query:  387 EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            E+ GP + +L+FW KLI LI S+IEEDKNSY PVLNQFPQELNVG++SA  +W LFA D KYALEEHE+  LCKS+ Y+N HF+VKWLH  YV  +P  +GQVPEYPAWFE FV+QWL+EN+DVSLEFL  A  RDKKD FQ+ +EH+ FS SVVDVFTQL Q FE+++KLEC DP I   YM+RFAKTI KVL  YA+IL  DF  +   E   C+LMNN+QQ R+QLEK + AMGG+EL+ +AA  L +LQ  LN VLDEL+ +F  SF  R+D  V  M  +L +V+G G     +++     AD +L+PLMD LDG+L+++A +C+K+VLKR+LKELW++VM ++E+ IVLPP TD+                        TG    F   +++ S +  +   + +E  R+L+PKQC+VL+ AL+ IKQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTD LIKT+V SQT Q S   +   VGEVS+QVDLFTHPGTGEHKVTVKV+                    ++GPH SDKKR   TKSK+ NW+PKYNE F F++GNE+   SYEL ICVKDYCF R+DR++G++V+ L+D+  +GSCACW  LGR+I MDETG TILRILSQR+NDEVA+EFVKLKSE R
Sbjct:  956 EEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLNQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDHLCKSADYMNLHFKVKWLHNEYVRDLPVLQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPSILAHYMRRFAKTIGKVLMQYADILSKDFPAYCTKEKLPCILMNNVQQLRVQLEKMFEAMGGKELDLEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPDARASAAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQ------------------------TGTQLIFTAAKEL-SHLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTTQGSGVDD--PVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPESYELQICVKDYCFAREDRVLGLAVMPLRDVTAKGSCACWCPLGRKIHMDETGLTILRILSQRSNDEVAREFVKLKSESR 1585          

HSP 2 Score: 516.153 bits (1328), Expect = 1.531e-158
Identity = 238/314 (75.80%), Postives = 277/314 (88.22%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNG-GNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKS KHGA+D+   II+AMK+RMK RER +PEIFE+IR++F+++   H++ M   +Q+VLDGTSKWS KI  TV+CAQGL AKDKTG+SDPYVTVQV K+KKRTKT+   LNP W EKF+FECHNSSDRIKVRVWDED+D+KS+++Q+  RESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYL +  G G V +PEARG+DAWK+YFDE  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  528 LQRAAEKSCKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVNKAAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVSKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVRIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 841          

BLAST of EMLSAG00000007474 vs. GO
Match: - (symbol:Unc13b "unc-13 homolog B (C. elegans)" species:10090 "Mus musculus" [GO:0001566 "non-kinase phorbol ester receptor activity" evidence=TAS] [GO:0005737 "cytoplasm" evidence=IEA] [GO:0005794 "Golgi apparatus" evidence=ISO] [GO:0005829 "cytosol" evidence=ISO] [GO:0006887 "exocytosis" evidence=IEA] [GO:0007165 "signal transduction" evidence=TAS] [GO:0007268 "synaptic transmission" evidence=IMP] [GO:0007528 "neuromuscular junction development" evidence=IGI] [GO:0016020 "membrane" evidence=IEA] [GO:0016081 "synaptic vesicle docking involved in exocytosis" evidence=IGI] [GO:0016082 "synaptic vesicle priming" evidence=IMP] [GO:0019992 "diacylglycerol binding" evidence=IEA] [GO:0030054 "cell junction" evidence=IEA] [GO:0031594 "neuromuscular junction" evidence=IDA] [GO:0035249 "synaptic transmission, glutamatergic" evidence=IGI;IMP] [GO:0035556 "intracellular signal transduction" evidence=IEA] [GO:0045202 "synapse" evidence=IEA] [GO:0046872 "metal ion binding" evidence=IEA] [GO:0048172 "regulation of short-term neuronal synaptic plasticity" evidence=IMP] [GO:0060384 "innervation" evidence=IGI] InterPro:IPR000008 InterPro:IPR002219 InterPro:IPR027080 Pfam:PF00130 Pfam:PF00168 PRINTS:PR00360 PROSITE:PS00479 PROSITE:PS50004 PROSITE:PS50081 SMART:SM00109 SMART:SM00239 MGI:MGI:1342278 GO:GO:0005794 GO:GO:0016020 GO:GO:0035556 GO:GO:0046872 GO:GO:0030054 GO:GO:0007528 GO:GO:0031594 GO:GO:0035249 SUPFAM:SSF49562 EMBL:AL732504 InterPro:IPR014770 InterPro:IPR014772 InterPro:IPR019558 Pfam:PF10540 PROSITE:PS51258 PROSITE:PS51259 GO:GO:0048172 GO:GO:0016081 GO:GO:0019992 GO:GO:0016082 InterPro:IPR010439 Pfam:PF06292 EMBL:AL672276 GO:GO:0060384 eggNOG:NOG245727 HOGENOM:HOG000231404 HOVERGEN:HBG057340 KO:K15293 OrthoDB:EOG76738V TreeFam:TF312844 PANTHER:PTHR10480 GeneTree:ENSGT00730000110590 CleanEx:MM_UNC13A CTD:10497 EMBL:AB162894 EMBL:AF115848 EMBL:AL772176 EMBL:BC157967 EMBL:BC158025 RefSeq:NP_001074882.1 RefSeq:NP_067443.2 RefSeq:XP_006537859.1 UniGene:Mm.128892 ProteinModelPortal:Q9Z1N9 SMR:Q9Z1N9 STRING:10090.ENSMUSP00000103586 PhosphoSite:Q9Z1N9 PaxDb:Q9Z1N9 PRIDE:Q9Z1N9 DNASU:22249 Ensembl:ENSMUST00000079978 Ensembl:ENSMUST00000107952 Ensembl:ENSMUST00000163653 GeneID:22249 KEGG:mmu:22249 UCSC:uc008spg.2 UCSC:uc012dcy.1 NextBio:302327 PRO:PR:Q9Z1N9 ArrayExpress:Q9Z1N9 Bgee:Q9Z1N9 CleanEx:MM_UNC13B Genevestigator:Q9Z1N9 GO:GO:0001566 Uniprot:Q9Z1N9)

HSP 1 Score: 753.821 bits (1945), Expect = 0.000e+0
Identity = 367/657 (55.86%), Postives = 472/657 (71.84%), Query Frame = 0

Query:  387 EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            E+ GP + +L+FW KLI LI S+IEEDKNSY PVL+QFPQELNVG++SA  +W LFA D KYALEEHE+ RLCKS+ Y+N HF+VKWLH  YV  +P  +GQVPEYPAWFE FV+QWL+EN+DVSLEFL  A  RDKKD FQ+ +EH+ FS SVVDVFTQL Q FE+++KLEC DP I   YM+RFAKTI KVL  YA+IL  +F  +   E   C+LMNN+QQ R+QLEK + AMGG+EL+ +AA  L +LQ  LN VLDEL+ +F  SF  R+D  V  M  +L +V+G G     +++ V   AD +L+PLMD LDG+L+++A +C+K+VLKR+LKELW++VM ++E+ IVLPP TD++     L    K +++ +K                        +   + +E  R+L+PKQC+VL+ AL+ IKQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTD LIKT+V SQT Q +   +   VGEVS+QVDLFTHPGTGEHKVTVKV+                    ++GPH SDKKR   TKSK+ NW+PKYNE F F++GNE+   +YEL ICVKDYCF R+DR+IG++V+ L+D+  +GSCACW  LGR+I MDETG TILRILSQR+NDEVA+EFVKLKSE R
Sbjct:  967 EEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTA-AKELSQLSK------------------------LKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGAGVDD--PVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESR 1596          

HSP 2 Score: 520.005 bits (1338), Expect = 5.362e-160
Identity = 241/314 (76.75%), Postives = 279/314 (88.85%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNG-GNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+D+   II+AMK+RMK RER +PEIFE+IR++F++S   H++ M   +Q+VLDGTSKWS KI  TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W EKF+FECHNSSDRIKVRVWDED+D+KS+++Q+  RESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYL +  G G V +PEARG+DAWK+YFDE  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  540 LQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 853          

BLAST of EMLSAG00000007474 vs. GO
Match: - (symbol:UNC13B "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0007528 "neuromuscular junction development" evidence=IEA] [GO:0016081 "synaptic vesicle docking involved in exocytosis" evidence=IEA] [GO:0016082 "synaptic vesicle priming" evidence=IEA] [GO:0019992 "diacylglycerol binding" evidence=IEA] [GO:0031594 "neuromuscular junction" evidence=IEA] [GO:0035249 "synaptic transmission, glutamatergic" evidence=IEA] [GO:0035556 "intracellular signal transduction" evidence=IEA] [GO:0046872 "metal ion binding" evidence=IEA] [GO:0048172 "regulation of short-term neuronal synaptic plasticity" evidence=IEA] [GO:0060384 "innervation" evidence=IEA] InterPro:IPR000008 InterPro:IPR002219 InterPro:IPR027080 Pfam:PF00130 Pfam:PF00168 PRINTS:PR00360 PROSITE:PS00479 PROSITE:PS50004 PROSITE:PS50081 SMART:SM00109 SMART:SM00239 GO:GO:0035556 GO:GO:0046872 GO:GO:0007528 GO:GO:0031594 GO:GO:0035249 SUPFAM:SSF49562 InterPro:IPR014770 InterPro:IPR014772 InterPro:IPR019558 Pfam:PF10540 PROSITE:PS51258 PROSITE:PS51259 GO:GO:0048172 GO:GO:0016081 GO:GO:0019992 GO:GO:0016082 InterPro:IPR010439 Pfam:PF06292 GO:GO:0060384 KO:K15293 OrthoDB:EOG76738V TreeFam:TF312844 PANTHER:PTHR10480 GeneTree:ENSGT00730000110590 OMA:FQGSPDK EMBL:AAEX03007949 EMBL:AAEX03007950 EMBL:AAEX03007951 RefSeq:XP_005626829.1 Ensembl:ENSCAFT00000003214 GeneID:100683511 Uniprot:F1P9F6)

HSP 1 Score: 752.666 bits (1942), Expect = 0.000e+0
Identity = 368/657 (56.01%), Postives = 469/657 (71.39%), Query Frame = 0

Query:  387 EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            E+ GP + +L+FW KLI LI S+IEEDKNSY  VLNQFPQELNVG++SA  +W LFA D KYALEEHE+ RLCKS+ Y+N HF+VKWLH  YV  +P  +GQVPEYP WFE FV+QWL+EN+DVSLEFL  A  RDKKD FQ+ +EH+ FS SVVDVFTQL Q FE+++KLEC DP I   YM+RFAKTI KVL  YANIL   F ++   E   C+LMNN+QQ R+QLEK + AMGG+EL+ +AA  L +LQ  LN VLDEL+ +F  SF  R+D  V  M  +L +V+G G     +++ V   AD +L+PLMD LDG+L+++A +C+K+VLKR+LKELW++VM ++E+ IVLPP TD+                        TG    F   +++ S +  +   + +E  RSL+PKQC VL+ AL+ IKQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTD LIKT+V SQT Q S   +   VGEVS+QVDLFTHPGTGEHKVTVKV+                    ++GPH S+KKR   TKSK+ NW+PKYNE F F++GNE+   +YEL ICVKDYCF R+DR++G++V+ L+D+  +GSCACW  LGR+I MDETG TILRILSQR+NDEVA+EFVKLKSE R
Sbjct:  956 EEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPVWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYANILSKSFPDYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGPGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQ------------------------TGTQLIFTAAKEL-SHLSKLKDHMVREETRSLTPKQCEVLDLALDTIKQYFHAGGNGLKKTFLEKSSDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGSGVDD--PVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSEKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESR 1585          

HSP 2 Score: 520.776 bits (1340), Expect = 2.846e-160
Identity = 241/314 (76.75%), Postives = 279/314 (88.85%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNG-GNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+D+   II+AMK+RMK RER +PEIFE+IR++F++S   H++ M   +Q+VLDGTSKWS KI  TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W EKF+FECHNSSDRIKVRVWDED+D+KS+++Q+  RESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYL +  G G V +PEARG+DAWK+YFDE  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  528 LQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVLIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 841          

BLAST of EMLSAG00000007474 vs. GO
Match: - (symbol:UNC13B "Uncharacterized protein" species:9913 "Bos taurus" [GO:0007528 "neuromuscular junction development" evidence=IEA] [GO:0016081 "synaptic vesicle docking involved in exocytosis" evidence=IEA] [GO:0016082 "synaptic vesicle priming" evidence=IEA] [GO:0019992 "diacylglycerol binding" evidence=IEA] [GO:0031594 "neuromuscular junction" evidence=IEA] [GO:0035249 "synaptic transmission, glutamatergic" evidence=IEA] [GO:0035556 "intracellular signal transduction" evidence=IEA] [GO:0046872 "metal ion binding" evidence=IEA] [GO:0048172 "regulation of short-term neuronal synaptic plasticity" evidence=IEA] [GO:0060384 "innervation" evidence=IEA] InterPro:IPR000008 InterPro:IPR002219 InterPro:IPR027080 Pfam:PF00130 Pfam:PF00168 PRINTS:PR00360 PROSITE:PS00479 PROSITE:PS50004 PROSITE:PS50081 SMART:SM00109 SMART:SM00239 GO:GO:0035556 GO:GO:0046872 GO:GO:0007528 GO:GO:0031594 GO:GO:0035249 SUPFAM:SSF49562 InterPro:IPR014770 InterPro:IPR014772 InterPro:IPR019558 Pfam:PF10540 PROSITE:PS51258 PROSITE:PS51259 GO:GO:0048172 GO:GO:0016081 GO:GO:0019992 GO:GO:0016082 InterPro:IPR010439 Pfam:PF06292 GO:GO:0060384 KO:K15293 OrthoDB:EOG76738V TreeFam:TF312844 PANTHER:PTHR10480 GeneTree:ENSGT00730000110590 CTD:10497 OMA:FQGSPDK EMBL:DAAA02023126 EMBL:DAAA02023127 EMBL:DAAA02023128 RefSeq:NP_001180168.1 UniGene:Bt.20019 ProteinModelPortal:F1MP30 Ensembl:ENSBTAT00000015153 GeneID:530692 KEGG:bta:530692 NextBio:20875309 ArrayExpress:F1MP30 Uniprot:F1MP30)

HSP 1 Score: 750.74 bits (1937), Expect = 0.000e+0
Identity = 366/657 (55.71%), Postives = 470/657 (71.54%), Query Frame = 0

Query:  387 EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            E+ GP + +L+FW KLI LI S+IEEDKNSY  VLNQFPQELNVG++SA  +W LFA D KYALEEHE+ RLCKS+ Y+N HF+VKWLH  YV  +P  +GQVPEYPAWFE FV+QWL+EN+DVSLEFL  A  RDKKD FQ+ +EH+ FS SVVDVFTQL Q FE+++KLEC DP I   YM+RF+KTI KVL  YA+IL  +F  +   E   C+LMNN+QQ R+QLEK + AMGG+EL+ + A  L +LQ  LN VLDEL+ +F  SF  R+D  V  M  +L +V+G G     +++ V   AD +L+PLMD LDG+L+++A +C+K+VLKR+LKELW++VM ++E+ IVLPP TD+                        TG    F   +++ S +  +   + +E  R+L+PKQC+VL+ AL+ IKQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTD LIKT+V SQT Q S   +   VGEVS+QVDLFTHPGTGEHKVTVKV+                    ++GPH SDKKR   TKSK+ NW+PKYNE F F++GNE+   +YEL ICVKDYCF R+DR++G++V+ L+D+  +GSCACW  LGR+I MDETG TILRILSQR+NDEVA+EFVKLKSE R
Sbjct:  956 EEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTLVLNQFPQELNVGKVSAEVMWQLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVQDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFSKTIGKVLMEYADILSKNFPAYCTKEKMPCILMNNMQQLRVQLEKMFEAMGGKELDPETADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILSQVRGPGNASPNARASVTQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQ------------------------TGTQLIFTAAKEL-SQLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGSGVDD--PVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVLGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGVTILRILSQRSNDEVAREFVKLKSESR 1585          

HSP 2 Score: 521.546 bits (1342), Expect = 1.672e-160
Identity = 241/314 (76.75%), Postives = 279/314 (88.85%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNG-GNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+D+   II+AMK+RMK RER +PEIFE+IR++F++S   H++ M   +Q+VLDGTSKWS KI  TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W EKF+FECHNSSDRIKVRVWDED+D+KS+++Q+  RESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYL +  G G V +PEARG+DAWK+YFDE  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  528 LQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFNVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVQIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 841          

BLAST of EMLSAG00000007474 vs. GO
Match: - (symbol:UNC13B "Protein unc-13 homolog B" species:9606 "Homo sapiens" [GO:0007268 "synaptic transmission" evidence=IEA] [GO:0019992 "diacylglycerol binding" evidence=IEA] [GO:0035556 "intracellular signal transduction" evidence=IEA] [GO:0046872 "metal ion binding" evidence=IEA] InterPro:IPR000008 InterPro:IPR002219 InterPro:IPR027080 Pfam:PF00130 Pfam:PF00168 PRINTS:PR00360 PROSITE:PS00479 PROSITE:PS50004 PROSITE:PS50081 SMART:SM00109 SMART:SM00239 GO:GO:0035556 GO:GO:0046872 GO:GO:0007268 SUPFAM:SSF49562 InterPro:IPR014770 InterPro:IPR014772 InterPro:IPR019558 Pfam:PF10540 PROSITE:PS51258 PROSITE:PS51259 GO:GO:0019992 InterPro:IPR010439 Pfam:PF06292 EMBL:AL353795 KO:K15293 PANTHER:PTHR10480 EMBL:AL160274 EMBL:AL354669 UniGene:Hs.493791 GeneID:10497 KEGG:hsa:10497 CTD:10497 HGNC:HGNC:12566 RefSeq:XP_005251404.1 ProteinModelPortal:F8W8M9 SMR:F8W8M9 Ensembl:ENST00000378496 UCSC:uc003zwr.3 NextBio:35512933 ArrayExpress:F8W8M9 Bgee:F8W8M9 Uniprot:F8W8M9)

HSP 1 Score: 748.814 bits (1932), Expect = 0.000e+0
Identity = 371/674 (55.04%), Postives = 473/674 (70.18%), Query Frame = 0

Query:  387 EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQ------------DSSSSEEGS-----VGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            E+ GP + +L+FW KLI LI S+IEEDKNSY PVLNQFPQELNVG++SA  +W LFA D KYALEEHE+  LCKS+ Y+N HF+VKWLH  YV  +P  +GQVPEYPAWFE FV+QWL+EN+DVSLEFL  A  RDKKD FQ+ +EH+ FS SVVDVFTQL Q FE+++KLEC DP I   YM+RFAKTI KVL  YA+IL  DF  +   E   C+LMNN+QQ R+QLEK + AMGG+EL+ +AA  L +LQ  LN VLDEL+ +F  SF  R+D  V  M  +L +V+G G     +++     AD +L+PLMD LDG+L+++A +C+K+VLKR+LKELW++VM ++E+ IVLPP TD+                        TG    F   +++ S +  +   + +E  R+L+PKQC+VL+ AL+ IKQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTD LIKT+V SQT Q            +    E+GS     VGEVS+QVDLFTHPGTGEHKVTVKV+                    ++GPH SDKKR   TKSK+ NW+PKYNE F F++GNE+   SYEL ICVKDYCF R+DR++G++V+ L+D+  +GSCACW  LGR+I MDETG TILRILSQR+NDEVA+EFVKLKSE R
Sbjct:  956 EEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLNQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDHLCKSADYMNLHFKVKWLHNEYVRDLPVLQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPSILAHYMRRFAKTIGKVLMQYADILSKDFPAYCTKEKLPCILMNNVQQLRVQLEKMFEAMGGKELDLEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPDARASAAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQ------------------------TGTQLIFTAAKEL-SHLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTTQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPESYELQICVKDYCFAREDRVLGLAVMPLRDVTAKGSCACWCPLGRKIHMDETGLTILRILSQRSNDEVAREFVKLKSESR 1604          

HSP 2 Score: 515.768 bits (1327), Expect = 2.963e-158
Identity = 238/314 (75.80%), Postives = 277/314 (88.22%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNG-GNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKS KHGA+D+   II+AMK+RMK RER +PEIFE+IR++F+++   H++ M   +Q+VLDGTSKWS KI  TV+CAQGL AKDKTG+SDPYVTVQV K+KKRTKT+   LNP W EKF+FECHNSSDRIKVRVWDED+D+KS+++Q+  RESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYL +  G G V +PEARG+DAWK+YFDE  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  528 LQRAAEKSCKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVNKAAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVSKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVRIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 841          

BLAST of EMLSAG00000007474 vs. GO
Match: - (symbol:UNC13B "Protein unc-13 homolog B" species:9606 "Homo sapiens" [GO:0007268 "synaptic transmission" evidence=IEA] [GO:0019992 "diacylglycerol binding" evidence=IEA] [GO:0035556 "intracellular signal transduction" evidence=IEA] [GO:0046872 "metal ion binding" evidence=IEA] InterPro:IPR000008 InterPro:IPR002219 InterPro:IPR027080 Pfam:PF00130 Pfam:PF00168 PRINTS:PR00360 PROSITE:PS00479 PROSITE:PS50004 PROSITE:PS50081 SMART:SM00109 SMART:SM00239 GO:GO:0035556 GO:GO:0046872 GO:GO:0007268 SUPFAM:SSF49562 InterPro:IPR014770 InterPro:IPR014772 InterPro:IPR019558 Pfam:PF10540 PROSITE:PS51258 PROSITE:PS51259 GO:GO:0019992 InterPro:IPR010439 Pfam:PF06292 EMBL:AL353795 HOGENOM:HOG000231404 HOVERGEN:HBG057340 KO:K15293 OrthoDB:EOG76738V PANTHER:PTHR10480 EMBL:AL160274 EMBL:AL354669 UniGene:Hs.493791 GeneID:10497 KEGG:hsa:10497 CTD:10497 HGNC:HGNC:12566 RefSeq:XP_005251403.1 ProteinModelPortal:B1AM27 SMR:B1AM27 IntAct:B1AM27 PRIDE:B1AM27 Ensembl:ENST00000396787 OMA:FQGSPDK NextBio:35466712 ArrayExpress:B1AM27 Uniprot:B1AM27)

HSP 1 Score: 748.429 bits (1931), Expect = 0.000e+0
Identity = 371/674 (55.04%), Postives = 473/674 (70.18%), Query Frame = 0

Query:  387 EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQ------------DSSSSEEGS-----VGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            E+ GP + +L+FW KLI LI S+IEEDKNSY PVLNQFPQELNVG++SA  +W LFA D KYALEEHE+  LCKS+ Y+N HF+VKWLH  YV  +P  +GQVPEYPAWFE FV+QWL+EN+DVSLEFL  A  RDKKD FQ+ +EH+ FS SVVDVFTQL Q FE+++KLEC DP I   YM+RFAKTI KVL  YA+IL  DF  +   E   C+LMNN+QQ R+QLEK + AMGG+EL+ +AA  L +LQ  LN VLDEL+ +F  SF  R+D  V  M  +L +V+G G     +++     AD +L+PLMD LDG+L+++A +C+K+VLKR+LKELW++VM ++E+ IVLPP TD+                        TG    F   +++ S +  +   + +E  R+L+PKQC+VL+ AL+ IKQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTD LIKT+V SQT Q            +    E+GS     VGEVS+QVDLFTHPGTGEHKVTVKV+                    ++GPH SDKKR   TKSK+ NW+PKYNE F F++GNE+   SYEL ICVKDYCF R+DR++G++V+ L+D+  +GSCACW  LGR+I MDETG TILRILSQR+NDEVA+EFVKLKSE R
Sbjct:  968 EEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLNQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDHLCKSADYMNLHFKVKWLHNEYVRDLPVLQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPSILAHYMRRFAKTIGKVLMQYADILSKDFPAYCTKEKLPCILMNNVQQLRVQLEKMFEAMGGKELDLEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPDARASAAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQ------------------------TGTQLIFTAAKEL-SHLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTTQVHDGKGIRFTANEDIRPEKGSGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPESYELQICVKDYCFAREDRVLGLAVMPLRDVTAKGSCACWCPLGRKIHMDETGLTILRILSQRSNDEVAREFVKLKSESR 1616          

HSP 2 Score: 515.383 bits (1326), Expect = 4.776e-158
Identity = 238/314 (75.80%), Postives = 277/314 (88.22%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNG-GNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKS KHGA+D+   II+AMK+RMK RER +PEIFE+IR++F+++   H++ M   +Q+VLDGTSKWS KI  TV+CAQGL AKDKTG+SDPYVTVQV K+KKRTKT+   LNP W EKF+FECHNSSDRIKVRVWDED+D+KS+++Q+  RESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYL +  G G V +PEARG+DAWK+YFDE  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  540 LQRAAEKSCKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVNKAAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVSKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVRIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 853          

BLAST of EMLSAG00000007474 vs. GO
Match: - (symbol:Unc13b "Protein unc-13 homolog B" species:10116 "Rattus norvegicus" [GO:0007268 "synaptic transmission" evidence=IEA] [GO:0019992 "diacylglycerol binding" evidence=IEA] [GO:0035556 "intracellular signal transduction" evidence=IEA] [GO:0046872 "metal ion binding" evidence=IEA] InterPro:IPR000008 InterPro:IPR002219 InterPro:IPR027080 Pfam:PF00130 Pfam:PF00168 PRINTS:PR00360 PROSITE:PS00479 PROSITE:PS50004 PROSITE:PS50081 SMART:SM00109 SMART:SM00239 RGD:619723 GO:GO:0035556 GO:GO:0046872 GO:GO:0007268 SUPFAM:SSF49562 InterPro:IPR014770 InterPro:IPR014772 InterPro:IPR019558 Pfam:PF10540 PROSITE:PS51258 PROSITE:PS51259 GO:GO:0019992 InterPro:IPR010439 Pfam:PF06292 OrthoDB:EOG76738V PANTHER:PTHR10480 GeneTree:ENSGT00730000110590 EMBL:AABR06036758 EMBL:AABR06036759 EMBL:AABR06036760 EMBL:AABR06036761 EMBL:AABR06036762 EMBL:AABR06036763 EMBL:AABR06036764 EMBL:AABR06036765 Ensembl:ENSRNOT00000043216 NextBio:35578850 Uniprot:F1LXM4)

HSP 1 Score: 744.191 bits (1920), Expect = 0.000e+0
Identity = 366/674 (54.30%), Postives = 474/674 (70.33%), Query Frame = 0

Query:  387 EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQ------------DSSSSEEGS-----VGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            E+ GP + +L+FW KLI LI S+IEEDKNSY PVLNQFPQELNVG++SA  +W LFA D KYALEEHE+ RLCKS+ Y+N HF+VKWLH  YV  +P  +GQVPEYPAWFE FV+QWL+EN+DVSLEFL  A  RDK+D FQ+ +EH+ FS SVVDVFTQL Q FE+++KLEC DP I   YM+RFAKTI KVL  YA+IL  +F  +   E   C+LMNN+QQ R+QLEK + AMGG+EL+ +AA  L +LQ  LN VLDEL+ +F  SF  R+D  V  M  +L +V+G G     +++ V   AD +L+PLMD LDG+L+++A +C+K+VLKR+LKELW++VM ++E+ IVLPP  D++     L    K +++ +K                        +   + +E  R+L+PKQC+VL+ AL+ +KQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTD LIKT+V SQT Q            +    E+G+     VGEVS+QVDLFTHPGTGEHKVTVKV+                    ++GPH SDKKR   TKSK+ +W+PKYNE F F++GNE+   +YEL ICVKDYCF R+DR+IG++V+ L+D+  +GSCACW  LGR+I MDETG TILRILSQR+NDEVA+EFVKLKSE R
Sbjct:  968 EEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLNQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRELPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKRDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPSILAHYMRRFAKTIGKVLIQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLIDQTGTQLILTA-AKELSQLSK------------------------LKDHMVREETRNLTPKQCAVLDLALDTVKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNSWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESR 1616          

HSP 2 Score: 517.309 bits (1331), Expect = 8.448e-159
Identity = 239/314 (76.11%), Postives = 279/314 (88.85%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNG-GNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+D+   II+AMK+RMK RER +PEIFE+IR++F++S   H++ M   +Q+VLDGTSKWS KI  TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W EKF+FECHNSSDRIKVRVWDED+D+KS+++Q+  RESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRLQI+VEIKGEEKVAPYHVQYTCLHENLFHYL +  G G V +P+ARG+DAWK+YFDE  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  541 LQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQINVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPDARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 854          

BLAST of EMLSAG00000007474 vs. C. finmarchicus
Match: gi|592770253|gb|GAXK01184315.1| (TSA: Calanus finmarchicus comp134984_c1_seq7 transcribed RNA sequence)

HSP 1 Score: 1061.98 bits (2745), Expect = 0.000e+0
Identity = 548/677 (80.95%), Postives = 593/677 (87.59%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPT---ACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFF-KSFGGKQDXXXXXXXXXXDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVXXXXXXXXXXXXXXXXXXXXXQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQ 1029
            VD NEAKRD+EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELN+GQLSA+TIWGLFA D KYALEEHE  RLCKSSTYLNCHFRVKWL+KTYV+ VPPYKGQ+PEYPAWFE FVMQWLNENDDVSLEFLH AYARDKKD F+KNTEHSNFS+SVVDVFTQLTQCFEVLQKLECQ+P IW RYMKRFAKT+VKVL AYA++LK DFHNH+KDE T   ACVLMNNIQQTRIQLEKTY +MGGQELEEDAAS+LNQLQSTLNGVLD+LAH FA S + +VD SV  MGKLL +VKGGGQGQAISKSEV AAADGIL PLMDLLDGSLSMYAE CDKSVLKRLLK+LW+IVMK LE++IVLPP ++K  A+KN   D KN+AE  KKVED+T F  K    K D   +VM+VVSDISKEGE+SL+PKQC+VLE ALEVIKQYFHAGG GLKMSYVNKSEELKSLQHALSLYTQTTDALIK +VSSQTHQDSSS+ EGSVGEVSVQVDL+THPGTGEHKVTVKV+                    LIGPHL+DKKR   TK+KNANWSPKYNE F FIIGNE+Q+ +YELHI VKDYCFGRDDRLIGVSV+QLKDI DQGSCACWLSLGRRIQMDETGWTILRILSQRT DEVAKEFVKLKSEVR ++PLQ
Sbjct:  821 VDRNEAKRDAEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNIGQLSASTIWGLFATDMKYALEEHEAHRLCKSSTYLNCHFRVKWLYKTYVTDVPPYKGQIPEYPAWFEAFVMQWLNENDDVSLEFLHGAYARDKKDGFRKNTEHSNFSSSVVDVFTQLTQCFEVLQKLECQNPAIWNRYMKRFAKTVVKVLMAYADLLKNDFHNHLKDERTLTRACVLMNNIQQTRIQLEKTYQSMGGQELEEDAASILNQLQSTLNGVLDQLAHKFAESISPKVDNSVKKMGKLLQDVKGGGQGQAISKSEVAAAADGILGPLMDLLDGSLSMYAENCDKSVLKRLLKQLWRIVMKCLERSIVLPPMSEKK-ALKNF--DAKNVAESAKKVEDMTSFLMKGMSAKAD-VKNVMNVVSDISKEGEKSLTPKQCAVLENALEVIKQYFHAGGNGLKMSYVNKSEELKSLQHALSLYTQTTDALIKNFVSSQTHQDSSSTTEGSVGEVSVQVDLYTHPGTGEHKVTVKVVAANDLKTPKTQTFKPYVEVNLIGPHLADKKRKFATKNKNANWSPKYNETFHFIIGNEEQMTAYELHIGVKDYCFGRDDRLIGVSVMQLKDIMDQGSCACWLSLGRRIQMDETGWTILRILSQRTTDEVAKEFVKLKSEVRSDEPLQ 2839          

HSP 2 Score: 553.903 bits (1426), Expect = 3.000e-168
Identity = 266/314 (84.71%), Postives = 295/314 (93.95%), Query Frame = 0

Query:   68 AAEKSSKHGADDKANTIIIAXXXXXXXXXXXXPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            AAEKSSKHGADDKANTIIIAMKERMKERE+ERPE+FELIR +F++ PD HI+ +   EQ VLDGTSKWSCKIA  V+CAQGLIAKDKTGTSDPYVTVQVGK+KKRTKTVPQELNPEWNEKF FECHNSSDRIKVRVWDEDNDLK++LRQK TRESDDFLGQTIIEVRTLSGEMDVWY+LEKRSD+SSVSG+IRLQISVEIKGEE+VAPYHVQYTCLHENLFHYLCEKNGG VSLP+A+GE++WKIYF+E  +EIVDEFALRYG+ESI+QAMTHF+CLS+KYLCP VPA MSTLLANINA+YAHTTA+TNV  ++
Sbjct: 3149 AAEKSSKHGADDKANTIIIAMKERMKEREKERPEVFELIRTVFNVGPDTHIDNLEASEQVVLDGTSKWSCKIAVNVVCAQGLIAKDKTGTSDPYVTVQVGKTKKRTKTVPQELNPEWNEKFSFECHNSSDRIKVRVWDEDNDLKARLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYHLEKRSDRSSVSGSIRLQISVEIKGEEQVAPYHVQYTCLHENLFHYLCEKNGGEVSLPDAKGEESWKIYFEEPNEEIVDEFALRYGIESIYQAMTHFNCLSTKYLCPRVPASMSTLLANINAYYAHTTASTNVSASD 4090          

HSP 3 Score: 85.1149 bits (209), Expect = 1.010e-15
Identity = 47/88 (53.41%), Postives = 52/88 (59.09%), Query Frame = 0

Query:    7 LNEKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL---------------------------KLHVYKKXLQALIYPISS 67
            + EK+G  ++ R   DNPFFKDIDSMP EFRPRRKSIPLVSEL                           K+HVYKK LQALIYPISS
Sbjct: 4307 IEEKSGAAQAGRPGPDNPFFKDIDSMP-EFRPRRKSIPLVSELVLKTMAAKRNASGLSALPRPSLDNEELKMHVYKKTLQALIYPISS 4567          

BLAST of EMLSAG00000007474 vs. C. finmarchicus
Match: gi|592770255|gb|GAXK01184313.1| (TSA: Calanus finmarchicus comp134984_c1_seq5 transcribed RNA sequence)

HSP 1 Score: 1061.98 bits (2745), Expect = 0.000e+0
Identity = 548/677 (80.95%), Postives = 593/677 (87.59%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPT---ACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFF-KSFGGKQDXXXXXXXXXXDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVXXXXXXXXXXXXXXXXXXXXXQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQ 1029
            VD NEAKRD+EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELN+GQLSA+TIWGLFA D KYALEEHE  RLCKSSTYLNCHFRVKWL+KTYV+ VPPYKGQ+PEYPAWFE FVMQWLNENDDVSLEFLH AYARDKKD F+KNTEHSNFS+SVVDVFTQLTQCFEVLQKLECQ+P IW RYMKRFAKT+VKVL AYA++LK DFHNH+KDE T   ACVLMNNIQQTRIQLEKTY +MGGQELEEDAAS+LNQLQSTLNGVLD+LAH FA S + +VD SV  MGKLL +VKGGGQGQAISKSEV AAADGIL PLMDLLDGSLSMYAE CDKSVLKRLLK+LW+IVMK LE++IVLPP ++K  A+KN   D KN+AE  KKVED+T F  K    K D   +VM+VVSDISKEGE+SL+PKQC+VLE ALEVIKQYFHAGG GLKMSYVNKSEELKSLQHALSLYTQTTDALIK +VSSQTHQDSSS+ EGSVGEVSVQVDL+THPGTGEHKVTVKV+                    LIGPHL+DKKR   TK+KNANWSPKYNE F FIIGNE+Q+ +YELHI VKDYCFGRDDRLIGVSV+QLKDI DQGSCACWLSLGRRIQMDETGWTILRILSQRT DEVAKEFVKLKSEVR ++PLQ
Sbjct:  821 VDRNEAKRDAEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNIGQLSASTIWGLFATDMKYALEEHEAHRLCKSSTYLNCHFRVKWLYKTYVTDVPPYKGQIPEYPAWFEAFVMQWLNENDDVSLEFLHGAYARDKKDGFRKNTEHSNFSSSVVDVFTQLTQCFEVLQKLECQNPAIWNRYMKRFAKTVVKVLMAYADLLKNDFHNHLKDERTLTRACVLMNNIQQTRIQLEKTYQSMGGQELEEDAASILNQLQSTLNGVLDQLAHKFAESISPKVDNSVKKMGKLLQDVKGGGQGQAISKSEVAAAADGILGPLMDLLDGSLSMYAENCDKSVLKRLLKQLWRIVMKCLERSIVLPPMSEKK-ALKNF--DAKNVAESAKKVEDMTSFLMKGMSAKAD-VKNVMNVVSDISKEGEKSLTPKQCAVLENALEVIKQYFHAGGNGLKMSYVNKSEELKSLQHALSLYTQTTDALIKNFVSSQTHQDSSSTTEGSVGEVSVQVDLYTHPGTGEHKVTVKVVAANDLKTPKTQTFKPYVEVNLIGPHLADKKRKFATKNKNANWSPKYNETFHFIIGNEEQMTAYELHIGVKDYCFGRDDRLIGVSVMQLKDIMDQGSCACWLSLGRRIQMDETGWTILRILSQRTTDEVAKEFVKLKSEVRSDEPLQ 2839          

HSP 2 Score: 553.903 bits (1426), Expect = 3.334e-168
Identity = 266/314 (84.71%), Postives = 295/314 (93.95%), Query Frame = 0

Query:   68 AAEKSSKHGADDKANTIIIAXXXXXXXXXXXXPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            AAEKSSKHGADDKANTIIIAMKERMKERE+ERPE+FELIR +F++ PD HI+ +   EQ VLDGTSKWSCKIA  V+CAQGLIAKDKTGTSDPYVTVQVGK+KKRTKTVPQELNPEWNEKF FECHNSSDRIKVRVWDEDNDLK++LRQK TRESDDFLGQTIIEVRTLSGEMDVWY+LEKRSD+SSVSG+IRLQISVEIKGEE+VAPYHVQYTCLHENLFHYLCEKNGG VSLP+A+GE++WKIYF+E  +EIVDEFALRYG+ESI+QAMTHF+CLS+KYLCP VPA MSTLLANINA+YAHTTA+TNV  ++
Sbjct: 3149 AAEKSSKHGADDKANTIIIAMKERMKEREKERPEVFELIRTVFNVGPDTHIDNLEASEQVVLDGTSKWSCKIAVNVVCAQGLIAKDKTGTSDPYVTVQVGKTKKRTKTVPQELNPEWNEKFSFECHNSSDRIKVRVWDEDNDLKARLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYHLEKRSDRSSVSGSIRLQISVEIKGEEQVAPYHVQYTCLHENLFHYLCEKNGGEVSLPDAKGEESWKIYFEEPNEEIVDEFALRYGIESIYQAMTHFNCLSTKYLCPRVPASMSTLLANINAYYAHTTASTNVSASD 4090          

HSP 3 Score: 85.1149 bits (209), Expect = 1.013e-15
Identity = 47/88 (53.41%), Postives = 52/88 (59.09%), Query Frame = 0

Query:    7 LNEKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL---------------------------KLHVYKKXLQALIYPISS 67
            + EK+G  ++ R   DNPFFKDIDSMP EFRPRRKSIPLVSEL                           K+HVYKK LQALIYPISS
Sbjct: 4307 IEEKSGAAQAGRPGPDNPFFKDIDSMP-EFRPRRKSIPLVSELVLKTMAAKRNASGLSALPRPSLDNEELKMHVYKKTLQALIYPISS 4567          

BLAST of EMLSAG00000007474 vs. C. finmarchicus
Match: gi|592770257|gb|GAXK01184311.1| (TSA: Calanus finmarchicus comp134984_c1_seq3 transcribed RNA sequence)

HSP 1 Score: 1061.98 bits (2745), Expect = 0.000e+0
Identity = 548/677 (80.95%), Postives = 593/677 (87.59%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPT---ACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFF-KSFGGKQDXXXXXXXXXXDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVXXXXXXXXXXXXXXXXXXXXXQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQ 1029
            VD NEAKRD+EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELN+GQLSA+TIWGLFA D KYALEEHE  RLCKSSTYLNCHFRVKWL+KTYV+ VPPYKGQ+PEYPAWFE FVMQWLNENDDVSLEFLH AYARDKKD F+KNTEHSNFS+SVVDVFTQLTQCFEVLQKLECQ+P IW RYMKRFAKT+VKVL AYA++LK DFHNH+KDE T   ACVLMNNIQQTRIQLEKTY +MGGQELEEDAAS+LNQLQSTLNGVLD+LAH FA S + +VD SV  MGKLL +VKGGGQGQAISKSEV AAADGIL PLMDLLDGSLSMYAE CDKSVLKRLLK+LW+IVMK LE++IVLPP ++K  A+KN   D KN+AE  KKVED+T F  K    K D   +VM+VVSDISKEGE+SL+PKQC+VLE ALEVIKQYFHAGG GLKMSYVNKSEELKSLQHALSLYTQTTDALIK +VSSQTHQDSSS+ EGSVGEVSVQVDL+THPGTGEHKVTVKV+                    LIGPHL+DKKR   TK+KNANWSPKYNE F FIIGNE+Q+ +YELHI VKDYCFGRDDRLIGVSV+QLKDI DQGSCACWLSLGRRIQMDETGWTILRILSQRT DEVAKEFVKLKSEVR ++PLQ
Sbjct: 1460 VDRNEAKRDAEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNIGQLSASTIWGLFATDMKYALEEHEAHRLCKSSTYLNCHFRVKWLYKTYVTDVPPYKGQIPEYPAWFEAFVMQWLNENDDVSLEFLHGAYARDKKDGFRKNTEHSNFSSSVVDVFTQLTQCFEVLQKLECQNPAIWNRYMKRFAKTVVKVLMAYADLLKNDFHNHLKDERTLTRACVLMNNIQQTRIQLEKTYQSMGGQELEEDAASILNQLQSTLNGVLDQLAHKFAESISPKVDNSVKKMGKLLQDVKGGGQGQAISKSEVAAAADGILGPLMDLLDGSLSMYAENCDKSVLKRLLKQLWRIVMKCLERSIVLPPMSEKK-ALKNF--DAKNVAESAKKVEDMTSFLMKGMSAKAD-VKNVMNVVSDISKEGEKSLTPKQCAVLENALEVIKQYFHAGGNGLKMSYVNKSEELKSLQHALSLYTQTTDALIKNFVSSQTHQDSSSTTEGSVGEVSVQVDLYTHPGTGEHKVTVKVVAANDLKTPKTQTFKPYVEVNLIGPHLADKKRKFATKNKNANWSPKYNETFHFIIGNEEQMTAYELHIGVKDYCFGRDDRLIGVSVMQLKDIMDQGSCACWLSLGRRIQMDETGWTILRILSQRTTDEVAKEFVKLKSEVRSDEPLQ 3478          

HSP 2 Score: 553.903 bits (1426), Expect = 3.748e-168
Identity = 266/314 (84.71%), Postives = 295/314 (93.95%), Query Frame = 0

Query:   68 AAEKSSKHGADDKANTIIIAXXXXXXXXXXXXPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            AAEKSSKHGADDKANTIIIAMKERMKERE+ERPE+FELIR +F++ PD HI+ +   EQ VLDGTSKWSCKIA  V+CAQGLIAKDKTGTSDPYVTVQVGK+KKRTKTVPQELNPEWNEKF FECHNSSDRIKVRVWDEDNDLK++LRQK TRESDDFLGQTIIEVRTLSGEMDVWY+LEKRSD+SSVSG+IRLQISVEIKGEE+VAPYHVQYTCLHENLFHYLCEKNGG VSLP+A+GE++WKIYF+E  +EIVDEFALRYG+ESI+QAMTHF+CLS+KYLCP VPA MSTLLANINA+YAHTTA+TNV  ++
Sbjct: 3788 AAEKSSKHGADDKANTIIIAMKERMKEREKERPEVFELIRTVFNVGPDTHIDNLEASEQVVLDGTSKWSCKIAVNVVCAQGLIAKDKTGTSDPYVTVQVGKTKKRTKTVPQELNPEWNEKFSFECHNSSDRIKVRVWDEDNDLKARLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYHLEKRSDRSSVSGSIRLQISVEIKGEEQVAPYHVQYTCLHENLFHYLCEKNGGEVSLPDAKGEESWKIYFEEPNEEIVDEFALRYGIESIYQAMTHFNCLSTKYLCPRVPASMSTLLANINAYYAHTTASTNVSASD 4729          

HSP 3 Score: 85.1149 bits (209), Expect = 1.017e-15
Identity = 47/88 (53.41%), Postives = 52/88 (59.09%), Query Frame = 0

Query:    7 LNEKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL---------------------------KLHVYKKXLQALIYPISS 67
            + EK+G  ++ R   DNPFFKDIDSMP EFRPRRKSIPLVSEL                           K+HVYKK LQALIYPISS
Sbjct: 4946 IEEKSGAAQAGRPGPDNPFFKDIDSMP-EFRPRRKSIPLVSELVLKTMAAKRNASGLSALPRPSLDNEELKMHVYKKTLQALIYPISS 5206          

BLAST of EMLSAG00000007474 vs. C. finmarchicus
Match: gi|592770259|gb|GAXK01184309.1| (TSA: Calanus finmarchicus comp134984_c1_seq1 transcribed RNA sequence)

HSP 1 Score: 1061.98 bits (2745), Expect = 0.000e+0
Identity = 548/677 (80.95%), Postives = 593/677 (87.59%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPT---ACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFF-KSFGGKQDXXXXXXXXXXDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVXXXXXXXXXXXXXXXXXXXXXQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQ 1029
            VD NEAKRD+EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELN+GQLSA+TIWGLFA D KYALEEHE  RLCKSSTYLNCHFRVKWL+KTYV+ VPPYKGQ+PEYPAWFE FVMQWLNENDDVSLEFLH AYARDKKD F+KNTEHSNFS+SVVDVFTQLTQCFEVLQKLECQ+P IW RYMKRFAKT+VKVL AYA++LK DFHNH+KDE T   ACVLMNNIQQTRIQLEKTY +MGGQELEEDAAS+LNQLQSTLNGVLD+LAH FA S + +VD SV  MGKLL +VKGGGQGQAISKSEV AAADGIL PLMDLLDGSLSMYAE CDKSVLKRLLK+LW+IVMK LE++IVLPP ++K  A+KN   D KN+AE  KKVED+T F  K    K D   +VM+VVSDISKEGE+SL+PKQC+VLE ALEVIKQYFHAGG GLKMSYVNKSEELKSLQHALSLYTQTTDALIK +VSSQTHQDSSS+ EGSVGEVSVQVDL+THPGTGEHKVTVKV+                    LIGPHL+DKKR   TK+KNANWSPKYNE F FIIGNE+Q+ +YELHI VKDYCFGRDDRLIGVSV+QLKDI DQGSCACWLSLGRRIQMDETGWTILRILSQRT DEVAKEFVKLKSEVR ++PLQ
Sbjct: 1460 VDRNEAKRDAEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNIGQLSASTIWGLFATDMKYALEEHEAHRLCKSSTYLNCHFRVKWLYKTYVTDVPPYKGQIPEYPAWFEAFVMQWLNENDDVSLEFLHGAYARDKKDGFRKNTEHSNFSSSVVDVFTQLTQCFEVLQKLECQNPAIWNRYMKRFAKTVVKVLMAYADLLKNDFHNHLKDERTLTRACVLMNNIQQTRIQLEKTYQSMGGQELEEDAASILNQLQSTLNGVLDQLAHKFAESISPKVDNSVKKMGKLLQDVKGGGQGQAISKSEVAAAADGILGPLMDLLDGSLSMYAENCDKSVLKRLLKQLWRIVMKCLERSIVLPPMSEKK-ALKNF--DAKNVAESAKKVEDMTSFLMKGMSAKAD-VKNVMNVVSDISKEGEKSLTPKQCAVLENALEVIKQYFHAGGNGLKMSYVNKSEELKSLQHALSLYTQTTDALIKNFVSSQTHQDSSSTTEGSVGEVSVQVDLYTHPGTGEHKVTVKVVAANDLKTPKTQTFKPYVEVNLIGPHLADKKRKFATKNKNANWSPKYNETFHFIIGNEEQMTAYELHIGVKDYCFGRDDRLIGVSVMQLKDIMDQGSCACWLSLGRRIQMDETGWTILRILSQRTTDEVAKEFVKLKSEVRSDEPLQ 3478          

HSP 2 Score: 553.903 bits (1426), Expect = 4.051e-168
Identity = 266/314 (84.71%), Postives = 295/314 (93.95%), Query Frame = 0

Query:   68 AAEKSSKHGADDKANTIIIAXXXXXXXXXXXXPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            AAEKSSKHGADDKANTIIIAMKERMKERE+ERPE+FELIR +F++ PD HI+ +   EQ VLDGTSKWSCKIA  V+CAQGLIAKDKTGTSDPYVTVQVGK+KKRTKTVPQELNPEWNEKF FECHNSSDRIKVRVWDEDNDLK++LRQK TRESDDFLGQTIIEVRTLSGEMDVWY+LEKRSD+SSVSG+IRLQISVEIKGEE+VAPYHVQYTCLHENLFHYLCEKNGG VSLP+A+GE++WKIYF+E  +EIVDEFALRYG+ESI+QAMTHF+CLS+KYLCP VPA MSTLLANINA+YAHTTA+TNV  ++
Sbjct: 3788 AAEKSSKHGADDKANTIIIAMKERMKEREKERPEVFELIRTVFNVGPDTHIDNLEASEQVVLDGTSKWSCKIAVNVVCAQGLIAKDKTGTSDPYVTVQVGKTKKRTKTVPQELNPEWNEKFSFECHNSSDRIKVRVWDEDNDLKARLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYHLEKRSDRSSVSGSIRLQISVEIKGEEQVAPYHVQYTCLHENLFHYLCEKNGGEVSLPDAKGEESWKIYFEEPNEEIVDEFALRYGIESIYQAMTHFNCLSTKYLCPRVPASMSTLLANINAYYAHTTASTNVSASD 4729          

HSP 3 Score: 85.1149 bits (209), Expect = 1.020e-15
Identity = 47/88 (53.41%), Postives = 52/88 (59.09%), Query Frame = 0

Query:    7 LNEKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL---------------------------KLHVYKKXLQALIYPISS 67
            + EK+G  ++ R   DNPFFKDIDSMP EFRPRRKSIPLVSEL                           K+HVYKK LQALIYPISS
Sbjct: 4946 IEEKSGAAQAGRPGPDNPFFKDIDSMP-EFRPRRKSIPLVSELVLKTMAAKRNASGLSALPRPSLDNEELKMHVYKKTLQALIYPISS 5206          

BLAST of EMLSAG00000007474 vs. C. finmarchicus
Match: gi|592770252|gb|GAXK01184316.1| (TSA: Calanus finmarchicus comp134984_c1_seq8 transcribed RNA sequence)

HSP 1 Score: 1060.83 bits (2742), Expect = 0.000e+0
Identity = 548/677 (80.95%), Postives = 593/677 (87.59%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPT---ACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFF-KSFGGKQDXXXXXXXXXXDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVXXXXXXXXXXXXXXXXXXXXXQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQ 1029
            VD NEAKRD+EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELN+GQLSA+TIWGLFA D KYALEEHE  RLCKSSTYLNCHFRVKWL+KTYV+ VPPYKGQ+PEYPAWFE FVMQWLNENDDVSLEFLH AYARDKKD F+KNTEHSNFS+SVVDVFTQLTQCFEVLQKLECQ+P IW RYMKRFAKT+VKVL AYA++LK DFHNH+KDE T   ACVLMNNIQQTRIQLEKTY +MGGQELEEDAAS+LNQLQSTLNGVLD+LAH FA S + +VD SV  MGKLL +VKGGGQGQAISKSEV AAADGIL PLMDLLDGSLSMYAE CDKSVLKRLLK+LW+IVMK LE++IVLPP ++K  A+KN   D KN+AE  KKVED+T F  K    K D   +VM+VVSDISKEGE+SL+PKQC+VLE ALEVIKQYFHAGG GLKMSYVNKSEELKSLQHALSLYTQTTDALIK +VSSQTHQDSSS+ EGSVGEVSVQVDL+THPGTGEHKVTVKV+                    LIGPHL+DKKR   TK+KNANWSPKYNE F FIIGNE+Q+ +YELHI VKDYCFGRDDRLIGVSV+QLKDI DQGSCACWLSLGRRIQMDETGWTILRILSQRT DEVAKEFVKLKSEVR ++PLQ
Sbjct:  821 VDRNEAKRDAEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNIGQLSASTIWGLFATDMKYALEEHEAHRLCKSSTYLNCHFRVKWLYKTYVTDVPPYKGQIPEYPAWFEAFVMQWLNENDDVSLEFLHGAYARDKKDGFRKNTEHSNFSSSVVDVFTQLTQCFEVLQKLECQNPAIWNRYMKRFAKTVVKVLMAYADLLKNDFHNHLKDERTLTRACVLMNNIQQTRIQLEKTYQSMGGQELEEDAASILNQLQSTLNGVLDQLAHKFAESISPKVDNSVKKMGKLLQDVKGGGQGQAISKSEVAAAADGILGPLMDLLDGSLSMYAENCDKSVLKRLLKQLWRIVMKCLERSIVLPPMSEKK-ALKNF--DAKNVAESAKKVEDMTSFLMKGMSAKAD-VKNVMNVVSDISKEGEKSLTPKQCAVLENALEVIKQYFHAGGNGLKMSYVNKSEELKSLQHALSLYTQTTDALIKNFVSSQTHQDSSSTTEGSVGEVSVQVDLYTHPGTGEHKVTVKVVAANDLKTPKTQTFKPYVEVNLIGPHLADKKRKFATKNKNANWSPKYNETFHFIIGNEEQMTAYELHIGVKDYCFGRDDRLIGVSVMQLKDIMDQGSCACWLSLGRRIQMDETGWTILRILSQRTTDEVAKEFVKLKSEVRSDEPLQ 2839          

HSP 2 Score: 553.903 bits (1426), Expect = 3.236e-168
Identity = 266/317 (83.91%), Postives = 296/317 (93.38%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAXXXXXXXXXXXXPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            +  AAEKSSKHGADDKANTIIIAMKERMKERE+ERPE+FELIR +F++ PD HI+ +   EQ VLDGTSKWSCKIA  V+CAQGLIAKDKTGTSDPYVTVQVGK+KKRTKTVPQELNPEWNEKF FECHNSSDRIKVRVWDEDNDLK++LRQK TRESDDFLGQTIIEVRTLSGEMDVWY+LEKRSD+SSVSG+IRLQISVEIKGEE+VAPYHVQYTCLHENLFHYLCEKNGG VSLP+A+GE++WKIYF+E  +EIVDEFALRYG+ESI+QAMTHF+CLS+KYLCP VPA MSTLLANINA+YAHTTA+TNV  ++
Sbjct: 3149 LQRAAEKSSKHGADDKANTIIIAMKERMKEREKERPEVFELIRTVFNVGPDTHIDNLEASEQVVLDGTSKWSCKIAVNVVCAQGLIAKDKTGTSDPYVTVQVGKTKKRTKTVPQELNPEWNEKFSFECHNSSDRIKVRVWDEDNDLKARLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYHLEKRSDRSSVSGSIRLQISVEIKGEEQVAPYHVQYTCLHENLFHYLCEKNGGEVSLPDAKGEESWKIYFEEPNEEIVDEFALRYGIESIYQAMTHFNCLSTKYLCPRVPASMSTLLANINAYYAHTTASTNVSASD 4099          

HSP 3 Score: 85.1149 bits (209), Expect = 1.107e-15
Identity = 47/88 (53.41%), Postives = 52/88 (59.09%), Query Frame = 0

Query:    7 LNEKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL---------------------------KLHVYKKXLQALIYPISS 67
            + EK+G  ++ R   DNPFFKDIDSMP EFRPRRKSIPLVSEL                           K+HVYKK LQALIYPISS
Sbjct: 4259 IEEKSGAAQAGRPGPDNPFFKDIDSMP-EFRPRRKSIPLVSELVLKTMAAKRNASGLSALPRPSLDNEELKMHVYKKTLQALIYPISS 4519          

BLAST of EMLSAG00000007474 vs. C. finmarchicus
Match: gi|592770254|gb|GAXK01184314.1| (TSA: Calanus finmarchicus comp134984_c1_seq6 transcribed RNA sequence)

HSP 1 Score: 1060.83 bits (2742), Expect = 0.000e+0
Identity = 548/677 (80.95%), Postives = 593/677 (87.59%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPT---ACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFF-KSFGGKQDXXXXXXXXXXDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVXXXXXXXXXXXXXXXXXXXXXQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQ 1029
            VD NEAKRD+EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELN+GQLSA+TIWGLFA D KYALEEHE  RLCKSSTYLNCHFRVKWL+KTYV+ VPPYKGQ+PEYPAWFE FVMQWLNENDDVSLEFLH AYARDKKD F+KNTEHSNFS+SVVDVFTQLTQCFEVLQKLECQ+P IW RYMKRFAKT+VKVL AYA++LK DFHNH+KDE T   ACVLMNNIQQTRIQLEKTY +MGGQELEEDAAS+LNQLQSTLNGVLD+LAH FA S + +VD SV  MGKLL +VKGGGQGQAISKSEV AAADGIL PLMDLLDGSLSMYAE CDKSVLKRLLK+LW+IVMK LE++IVLPP ++K  A+KN   D KN+AE  KKVED+T F  K    K D   +VM+VVSDISKEGE+SL+PKQC+VLE ALEVIKQYFHAGG GLKMSYVNKSEELKSLQHALSLYTQTTDALIK +VSSQTHQDSSS+ EGSVGEVSVQVDL+THPGTGEHKVTVKV+                    LIGPHL+DKKR   TK+KNANWSPKYNE F FIIGNE+Q+ +YELHI VKDYCFGRDDRLIGVSV+QLKDI DQGSCACWLSLGRRIQMDETGWTILRILSQRT DEVAKEFVKLKSEVR ++PLQ
Sbjct:  821 VDRNEAKRDAEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNIGQLSASTIWGLFATDMKYALEEHEAHRLCKSSTYLNCHFRVKWLYKTYVTDVPPYKGQIPEYPAWFEAFVMQWLNENDDVSLEFLHGAYARDKKDGFRKNTEHSNFSSSVVDVFTQLTQCFEVLQKLECQNPAIWNRYMKRFAKTVVKVLMAYADLLKNDFHNHLKDERTLTRACVLMNNIQQTRIQLEKTYQSMGGQELEEDAASILNQLQSTLNGVLDQLAHKFAESISPKVDNSVKKMGKLLQDVKGGGQGQAISKSEVAAAADGILGPLMDLLDGSLSMYAENCDKSVLKRLLKQLWRIVMKCLERSIVLPPMSEKK-ALKNF--DAKNVAESAKKVEDMTSFLMKGMSAKAD-VKNVMNVVSDISKEGEKSLTPKQCAVLENALEVIKQYFHAGGNGLKMSYVNKSEELKSLQHALSLYTQTTDALIKNFVSSQTHQDSSSTTEGSVGEVSVQVDLYTHPGTGEHKVTVKVVAANDLKTPKTQTFKPYVEVNLIGPHLADKKRKFATKNKNANWSPKYNETFHFIIGNEEQMTAYELHIGVKDYCFGRDDRLIGVSVMQLKDIMDQGSCACWLSLGRRIQMDETGWTILRILSQRTTDEVAKEFVKLKSEVRSDEPLQ 2839          

HSP 2 Score: 553.903 bits (1426), Expect = 3.606e-168
Identity = 266/317 (83.91%), Postives = 296/317 (93.38%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAXXXXXXXXXXXXPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            +  AAEKSSKHGADDKANTIIIAMKERMKERE+ERPE+FELIR +F++ PD HI+ +   EQ VLDGTSKWSCKIA  V+CAQGLIAKDKTGTSDPYVTVQVGK+KKRTKTVPQELNPEWNEKF FECHNSSDRIKVRVWDEDNDLK++LRQK TRESDDFLGQTIIEVRTLSGEMDVWY+LEKRSD+SSVSG+IRLQISVEIKGEE+VAPYHVQYTCLHENLFHYLCEKNGG VSLP+A+GE++WKIYF+E  +EIVDEFALRYG+ESI+QAMTHF+CLS+KYLCP VPA MSTLLANINA+YAHTTA+TNV  ++
Sbjct: 3149 LQRAAEKSSKHGADDKANTIIIAMKERMKEREKERPEVFELIRTVFNVGPDTHIDNLEASEQVVLDGTSKWSCKIAVNVVCAQGLIAKDKTGTSDPYVTVQVGKTKKRTKTVPQELNPEWNEKFSFECHNSSDRIKVRVWDEDNDLKARLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYHLEKRSDRSSVSGSIRLQISVEIKGEEQVAPYHVQYTCLHENLFHYLCEKNGGEVSLPDAKGEESWKIYFEEPNEEIVDEFALRYGIESIYQAMTHFNCLSTKYLCPRVPASMSTLLANINAYYAHTTASTNVSASD 4099          

HSP 3 Score: 85.1149 bits (209), Expect = 1.111e-15
Identity = 47/88 (53.41%), Postives = 52/88 (59.09%), Query Frame = 0

Query:    7 LNEKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL---------------------------KLHVYKKXLQALIYPISS 67
            + EK+G  ++ R   DNPFFKDIDSMP EFRPRRKSIPLVSEL                           K+HVYKK LQALIYPISS
Sbjct: 4259 IEEKSGAAQAGRPGPDNPFFKDIDSMP-EFRPRRKSIPLVSELVLKTMAAKRNASGLSALPRPSLDNEELKMHVYKKTLQALIYPISS 4519          

BLAST of EMLSAG00000007474 vs. C. finmarchicus
Match: gi|592770256|gb|GAXK01184312.1| (TSA: Calanus finmarchicus comp134984_c1_seq4 transcribed RNA sequence)

HSP 1 Score: 1060.83 bits (2742), Expect = 0.000e+0
Identity = 548/677 (80.95%), Postives = 593/677 (87.59%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPT---ACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFF-KSFGGKQDXXXXXXXXXXDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVXXXXXXXXXXXXXXXXXXXXXQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQ 1029
            VD NEAKRD+EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELN+GQLSA+TIWGLFA D KYALEEHE  RLCKSSTYLNCHFRVKWL+KTYV+ VPPYKGQ+PEYPAWFE FVMQWLNENDDVSLEFLH AYARDKKD F+KNTEHSNFS+SVVDVFTQLTQCFEVLQKLECQ+P IW RYMKRFAKT+VKVL AYA++LK DFHNH+KDE T   ACVLMNNIQQTRIQLEKTY +MGGQELEEDAAS+LNQLQSTLNGVLD+LAH FA S + +VD SV  MGKLL +VKGGGQGQAISKSEV AAADGIL PLMDLLDGSLSMYAE CDKSVLKRLLK+LW+IVMK LE++IVLPP ++K  A+KN   D KN+AE  KKVED+T F  K    K D   +VM+VVSDISKEGE+SL+PKQC+VLE ALEVIKQYFHAGG GLKMSYVNKSEELKSLQHALSLYTQTTDALIK +VSSQTHQDSSS+ EGSVGEVSVQVDL+THPGTGEHKVTVKV+                    LIGPHL+DKKR   TK+KNANWSPKYNE F FIIGNE+Q+ +YELHI VKDYCFGRDDRLIGVSV+QLKDI DQGSCACWLSLGRRIQMDETGWTILRILSQRT DEVAKEFVKLKSEVR ++PLQ
Sbjct: 1460 VDRNEAKRDAEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNIGQLSASTIWGLFATDMKYALEEHEAHRLCKSSTYLNCHFRVKWLYKTYVTDVPPYKGQIPEYPAWFEAFVMQWLNENDDVSLEFLHGAYARDKKDGFRKNTEHSNFSSSVVDVFTQLTQCFEVLQKLECQNPAIWNRYMKRFAKTVVKVLMAYADLLKNDFHNHLKDERTLTRACVLMNNIQQTRIQLEKTYQSMGGQELEEDAASILNQLQSTLNGVLDQLAHKFAESISPKVDNSVKKMGKLLQDVKGGGQGQAISKSEVAAAADGILGPLMDLLDGSLSMYAENCDKSVLKRLLKQLWRIVMKCLERSIVLPPMSEKK-ALKNF--DAKNVAESAKKVEDMTSFLMKGMSAKAD-VKNVMNVVSDISKEGEKSLTPKQCAVLENALEVIKQYFHAGGNGLKMSYVNKSEELKSLQHALSLYTQTTDALIKNFVSSQTHQDSSSTTEGSVGEVSVQVDLYTHPGTGEHKVTVKVVAANDLKTPKTQTFKPYVEVNLIGPHLADKKRKFATKNKNANWSPKYNETFHFIIGNEEQMTAYELHIGVKDYCFGRDDRLIGVSVMQLKDIMDQGSCACWLSLGRRIQMDETGWTILRILSQRTTDEVAKEFVKLKSEVRSDEPLQ 3478          

HSP 2 Score: 553.903 bits (1426), Expect = 4.063e-168
Identity = 266/317 (83.91%), Postives = 296/317 (93.38%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAXXXXXXXXXXXXPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            +  AAEKSSKHGADDKANTIIIAMKERMKERE+ERPE+FELIR +F++ PD HI+ +   EQ VLDGTSKWSCKIA  V+CAQGLIAKDKTGTSDPYVTVQVGK+KKRTKTVPQELNPEWNEKF FECHNSSDRIKVRVWDEDNDLK++LRQK TRESDDFLGQTIIEVRTLSGEMDVWY+LEKRSD+SSVSG+IRLQISVEIKGEE+VAPYHVQYTCLHENLFHYLCEKNGG VSLP+A+GE++WKIYF+E  +EIVDEFALRYG+ESI+QAMTHF+CLS+KYLCP VPA MSTLLANINA+YAHTTA+TNV  ++
Sbjct: 3788 LQRAAEKSSKHGADDKANTIIIAMKERMKEREKERPEVFELIRTVFNVGPDTHIDNLEASEQVVLDGTSKWSCKIAVNVVCAQGLIAKDKTGTSDPYVTVQVGKTKKRTKTVPQELNPEWNEKFSFECHNSSDRIKVRVWDEDNDLKARLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYHLEKRSDRSSVSGSIRLQISVEIKGEEQVAPYHVQYTCLHENLFHYLCEKNGGEVSLPDAKGEESWKIYFEEPNEEIVDEFALRYGIESIYQAMTHFNCLSTKYLCPRVPASMSTLLANINAYYAHTTASTNVSASD 4738          

HSP 3 Score: 85.1149 bits (209), Expect = 1.115e-15
Identity = 47/88 (53.41%), Postives = 52/88 (59.09%), Query Frame = 0

Query:    7 LNEKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL---------------------------KLHVYKKXLQALIYPISS 67
            + EK+G  ++ R   DNPFFKDIDSMP EFRPRRKSIPLVSEL                           K+HVYKK LQALIYPISS
Sbjct: 4898 IEEKSGAAQAGRPGPDNPFFKDIDSMP-EFRPRRKSIPLVSELVLKTMAAKRNASGLSALPRPSLDNEELKMHVYKKTLQALIYPISS 5158          

BLAST of EMLSAG00000007474 vs. C. finmarchicus
Match: gi|592770258|gb|GAXK01184310.1| (TSA: Calanus finmarchicus comp134984_c1_seq2 transcribed RNA sequence)

HSP 1 Score: 1060.83 bits (2742), Expect = 0.000e+0
Identity = 548/677 (80.95%), Postives = 593/677 (87.59%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPT---ACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFF-KSFGGKQDXXXXXXXXXXDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVXXXXXXXXXXXXXXXXXXXXXQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQ 1029
            VD NEAKRD+EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELN+GQLSA+TIWGLFA D KYALEEHE  RLCKSSTYLNCHFRVKWL+KTYV+ VPPYKGQ+PEYPAWFE FVMQWLNENDDVSLEFLH AYARDKKD F+KNTEHSNFS+SVVDVFTQLTQCFEVLQKLECQ+P IW RYMKRFAKT+VKVL AYA++LK DFHNH+KDE T   ACVLMNNIQQTRIQLEKTY +MGGQELEEDAAS+LNQLQSTLNGVLD+LAH FA S + +VD SV  MGKLL +VKGGGQGQAISKSEV AAADGIL PLMDLLDGSLSMYAE CDKSVLKRLLK+LW+IVMK LE++IVLPP ++K  A+KN   D KN+AE  KKVED+T F  K    K D   +VM+VVSDISKEGE+SL+PKQC+VLE ALEVIKQYFHAGG GLKMSYVNKSEELKSLQHALSLYTQTTDALIK +VSSQTHQDSSS+ EGSVGEVSVQVDL+THPGTGEHKVTVKV+                    LIGPHL+DKKR   TK+KNANWSPKYNE F FIIGNE+Q+ +YELHI VKDYCFGRDDRLIGVSV+QLKDI DQGSCACWLSLGRRIQMDETGWTILRILSQRT DEVAKEFVKLKSEVR ++PLQ
Sbjct: 1460 VDRNEAKRDAEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNIGQLSASTIWGLFATDMKYALEEHEAHRLCKSSTYLNCHFRVKWLYKTYVTDVPPYKGQIPEYPAWFEAFVMQWLNENDDVSLEFLHGAYARDKKDGFRKNTEHSNFSSSVVDVFTQLTQCFEVLQKLECQNPAIWNRYMKRFAKTVVKVLMAYADLLKNDFHNHLKDERTLTRACVLMNNIQQTRIQLEKTYQSMGGQELEEDAASILNQLQSTLNGVLDQLAHKFAESISPKVDNSVKKMGKLLQDVKGGGQGQAISKSEVAAAADGILGPLMDLLDGSLSMYAENCDKSVLKRLLKQLWRIVMKCLERSIVLPPMSEKK-ALKNF--DAKNVAESAKKVEDMTSFLMKGMSAKAD-VKNVMNVVSDISKEGEKSLTPKQCAVLENALEVIKQYFHAGGNGLKMSYVNKSEELKSLQHALSLYTQTTDALIKNFVSSQTHQDSSSTTEGSVGEVSVQVDLYTHPGTGEHKVTVKVVAANDLKTPKTQTFKPYVEVNLIGPHLADKKRKFATKNKNANWSPKYNETFHFIIGNEEQMTAYELHIGVKDYCFGRDDRLIGVSVMQLKDIMDQGSCACWLSLGRRIQMDETGWTILRILSQRTTDEVAKEFVKLKSEVRSDEPLQ 3478          

HSP 2 Score: 553.903 bits (1426), Expect = 4.400e-168
Identity = 266/317 (83.91%), Postives = 296/317 (93.38%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAXXXXXXXXXXXXPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            +  AAEKSSKHGADDKANTIIIAMKERMKERE+ERPE+FELIR +F++ PD HI+ +   EQ VLDGTSKWSCKIA  V+CAQGLIAKDKTGTSDPYVTVQVGK+KKRTKTVPQELNPEWNEKF FECHNSSDRIKVRVWDEDNDLK++LRQK TRESDDFLGQTIIEVRTLSGEMDVWY+LEKRSD+SSVSG+IRLQISVEIKGEE+VAPYHVQYTCLHENLFHYLCEKNGG VSLP+A+GE++WKIYF+E  +EIVDEFALRYG+ESI+QAMTHF+CLS+KYLCP VPA MSTLLANINA+YAHTTA+TNV  ++
Sbjct: 3788 LQRAAEKSSKHGADDKANTIIIAMKERMKEREKERPEVFELIRTVFNVGPDTHIDNLEASEQVVLDGTSKWSCKIAVNVVCAQGLIAKDKTGTSDPYVTVQVGKTKKRTKTVPQELNPEWNEKFSFECHNSSDRIKVRVWDEDNDLKARLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYHLEKRSDRSSVSGSIRLQISVEIKGEEQVAPYHVQYTCLHENLFHYLCEKNGGEVSLPDAKGEESWKIYFEEPNEEIVDEFALRYGIESIYQAMTHFNCLSTKYLCPRVPASMSTLLANINAYYAHTTASTNVSASD 4738          

HSP 3 Score: 85.1149 bits (209), Expect = 1.119e-15
Identity = 47/88 (53.41%), Postives = 52/88 (59.09%), Query Frame = 0

Query:    7 LNEKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL---------------------------KLHVYKKXLQALIYPISS 67
            + EK+G  ++ R   DNPFFKDIDSMP EFRPRRKSIPLVSEL                           K+HVYKK LQALIYPISS
Sbjct: 4898 IEEKSGAAQAGRPGPDNPFFKDIDSMP-EFRPRRKSIPLVSELVLKTMAAKRNASGLSALPRPSLDNEELKMHVYKKTLQALIYPISS 5158          

BLAST of EMLSAG00000007474 vs. C. finmarchicus
Match: gi|592770249|gb|GAXK01184319.1| (TSA: Calanus finmarchicus comp134984_c1_seq11 transcribed RNA sequence)

HSP 1 Score: 1056.59 bits (2731), Expect = 0.000e+0
Identity = 548/677 (80.95%), Postives = 593/677 (87.59%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPT---ACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFF-KSFGGKQDXXXXXXXXXXDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVXXXXXXXXXXXXXXXXXXXXXQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQ 1029
            VD NEAKRD+EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELN+GQLSA+TIWGLFA D KYALEEHE  RLCKSSTYLNCHFRVKWL+KTYV+ VPPYKGQ+PEYPAWFE FVMQWLNENDDVSLEFLH AYARDKKD F+KNTEHSNFS+SVVDVFTQLTQCFEVLQKLECQ+P IW RYMKRFAKT+VKVL AYA++LK DFHNH+KDE T   ACVLMNNIQQTRIQLEKTY +MGGQELEEDAAS+LNQLQSTLNGVLD+LAH FA S + +VD SV  MGKLL +VKGGGQGQAISKSEV AAADGIL PLMDLLDGSLSMYAE CDKSVLKRLLK+LW+IVMK LE++IVLPP ++K  A+KN   D KN+AE  KKVED+T F  K    K D   +VM+VVSDISKEGE+SL+PKQC+VLE ALEVIKQYFHAGG GLKMSYVNKSEELKSLQHALSLYTQTTDALIK +VSSQTHQDSSS+ EGSVGEVSVQVDL+THPGTGEHKVTVKV+                    LIGPHL+DKKR   TK+KNANWSPKYNE F FIIGNE+Q+ +YELHI VKDYCFGRDDRLIGVSV+QLKDI DQGSCACWLSLGRRIQMDETGWTILRILSQRT DEVAKEFVKLKSEVR ++PLQ
Sbjct:  821 VDRNEAKRDAEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNIGQLSASTIWGLFATDMKYALEEHEAHRLCKSSTYLNCHFRVKWLYKTYVTDVPPYKGQIPEYPAWFEAFVMQWLNENDDVSLEFLHGAYARDKKDGFRKNTEHSNFSSSVVDVFTQLTQCFEVLQKLECQNPAIWNRYMKRFAKTVVKVLMAYADLLKNDFHNHLKDERTLTRACVLMNNIQQTRIQLEKTYQSMGGQELEEDAASILNQLQSTLNGVLDQLAHKFAESISPKVDNSVKKMGKLLQDVKGGGQGQAISKSEVAAAADGILGPLMDLLDGSLSMYAENCDKSVLKRLLKQLWRIVMKCLERSIVLPPMSEKK-ALKNF--DAKNVAESAKKVEDMTSFLMKGMSAKAD-VKNVMNVVSDISKEGEKSLTPKQCAVLENALEVIKQYFHAGGNGLKMSYVNKSEELKSLQHALSLYTQTTDALIKNFVSSQTHQDSSSTTEGSVGEVSVQVDLYTHPGTGEHKVTVKVVAANDLKTPKTQTFKPYVEVNLIGPHLADKKRKFATKNKNANWSPKYNETFHFIIGNEEQMTAYELHIGVKDYCFGRDDRLIGVSVMQLKDIMDQGSCACWLSLGRRIQMDETGWTILRILSQRTTDEVAKEFVKLKSEVRSDEPLQ 2839          

HSP 2 Score: 551.206 bits (1419), Expect = 1.707e-167
Identity = 266/314 (84.71%), Postives = 295/314 (93.95%), Query Frame = 0

Query:   68 AAEKSSKHGADDKANTIIIAXXXXXXXXXXXXPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            AAEKSSKHGADDKANTIIIAMKERMKERE+ERPE+FELIR +F++ PD HI+ +   EQ VLDGTSKWSCKIA  V+CAQGLIAKDKTGTSDPYVTVQVGK+KKRTKTVPQELNPEWNEKF FECHNSSDRIKVRVWDEDNDLK++LRQK TRESDDFLGQTIIEVRTLSGEMDVWY+LEKRSD+SSVSG+IRLQISVEIKGEE+VAPYHVQYTCLHENLFHYLCEKNGG VSLP+A+GE++WKIYF+E  +EIVDEFALRYG+ESI+QAMTHF+CLS+KYLCP VPA MSTLLANINA+YAHTTA+TNV  ++
Sbjct: 3149 AAEKSSKHGADDKANTIIIAMKERMKEREKERPEVFELIRTVFNVGPDTHIDNLEASEQVVLDGTSKWSCKIAVNVVCAQGLIAKDKTGTSDPYVTVQVGKTKKRTKTVPQELNPEWNEKFSFECHNSSDRIKVRVWDEDNDLKARLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYHLEKRSDRSSVSGSIRLQISVEIKGEEQVAPYHVQYTCLHENLFHYLCEKNGGEVSLPDAKGEESWKIYFEEPNEEIVDEFALRYGIESIYQAMTHFNCLSTKYLCPRVPASMSTLLANINAYYAHTTASTNVSASD 4090          

HSP 3 Score: 84.3445 bits (207), Expect = 1.665e-15
Identity = 47/86 (54.65%), Postives = 51/86 (59.30%), Query Frame = 0

Query:    9 EKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL---------------------------KLHVYKKXLQALIYPISS 67
            EK+G  ++ R   DNPFFKDIDSMP EFRPRRKSIPLVSEL                           K+HVYKK LQALIYPISS
Sbjct: 4307 EKSGAAQAGRPGPDNPFFKDIDSMP-EFRPRRKSIPLVSELVLKTMAAKRNASGLSALPRPSLDNEELKMHVYKKTLQALIYPISS 4561          

BLAST of EMLSAG00000007474 vs. C. finmarchicus
Match: gi|592770251|gb|GAXK01184317.1| (TSA: Calanus finmarchicus comp134984_c1_seq9 transcribed RNA sequence)

HSP 1 Score: 1056.59 bits (2731), Expect = 0.000e+0
Identity = 548/677 (80.95%), Postives = 593/677 (87.59%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPT---ACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFF-KSFGGKQDXXXXXXXXXXDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVXXXXXXXXXXXXXXXXXXXXXQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQ 1029
            VD NEAKRD+EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELN+GQLSA+TIWGLFA D KYALEEHE  RLCKSSTYLNCHFRVKWL+KTYV+ VPPYKGQ+PEYPAWFE FVMQWLNENDDVSLEFLH AYARDKKD F+KNTEHSNFS+SVVDVFTQLTQCFEVLQKLECQ+P IW RYMKRFAKT+VKVL AYA++LK DFHNH+KDE T   ACVLMNNIQQTRIQLEKTY +MGGQELEEDAAS+LNQLQSTLNGVLD+LAH FA S + +VD SV  MGKLL +VKGGGQGQAISKSEV AAADGIL PLMDLLDGSLSMYAE CDKSVLKRLLK+LW+IVMK LE++IVLPP ++K  A+KN   D KN+AE  KKVED+T F  K    K D   +VM+VVSDISKEGE+SL+PKQC+VLE ALEVIKQYFHAGG GLKMSYVNKSEELKSLQHALSLYTQTTDALIK +VSSQTHQDSSS+ EGSVGEVSVQVDL+THPGTGEHKVTVKV+                    LIGPHL+DKKR   TK+KNANWSPKYNE F FIIGNE+Q+ +YELHI VKDYCFGRDDRLIGVSV+QLKDI DQGSCACWLSLGRRIQMDETGWTILRILSQRT DEVAKEFVKLKSEVR ++PLQ
Sbjct: 1460 VDRNEAKRDAEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNIGQLSASTIWGLFATDMKYALEEHEAHRLCKSSTYLNCHFRVKWLYKTYVTDVPPYKGQIPEYPAWFEAFVMQWLNENDDVSLEFLHGAYARDKKDGFRKNTEHSNFSSSVVDVFTQLTQCFEVLQKLECQNPAIWNRYMKRFAKTVVKVLMAYADLLKNDFHNHLKDERTLTRACVLMNNIQQTRIQLEKTYQSMGGQELEEDAASILNQLQSTLNGVLDQLAHKFAESISPKVDNSVKKMGKLLQDVKGGGQGQAISKSEVAAAADGILGPLMDLLDGSLSMYAENCDKSVLKRLLKQLWRIVMKCLERSIVLPPMSEKK-ALKNF--DAKNVAESAKKVEDMTSFLMKGMSAKAD-VKNVMNVVSDISKEGEKSLTPKQCAVLENALEVIKQYFHAGGNGLKMSYVNKSEELKSLQHALSLYTQTTDALIKNFVSSQTHQDSSSTTEGSVGEVSVQVDLYTHPGTGEHKVTVKVVAANDLKTPKTQTFKPYVEVNLIGPHLADKKRKFATKNKNANWSPKYNETFHFIIGNEEQMTAYELHIGVKDYCFGRDDRLIGVSVMQLKDIMDQGSCACWLSLGRRIQMDETGWTILRILSQRTTDEVAKEFVKLKSEVRSDEPLQ 3478          

HSP 2 Score: 551.206 bits (1419), Expect = 2.569e-167
Identity = 266/314 (84.71%), Postives = 295/314 (93.95%), Query Frame = 0

Query:   68 AAEKSSKHGADDKANTIIIAXXXXXXXXXXXXPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            AAEKSSKHGADDKANTIIIAMKERMKERE+ERPE+FELIR +F++ PD HI+ +   EQ VLDGTSKWSCKIA  V+CAQGLIAKDKTGTSDPYVTVQVGK+KKRTKTVPQELNPEWNEKF FECHNSSDRIKVRVWDEDNDLK++LRQK TRESDDFLGQTIIEVRTLSGEMDVWY+LEKRSD+SSVSG+IRLQISVEIKGEE+VAPYHVQYTCLHENLFHYLCEKNGG VSLP+A+GE++WKIYF+E  +EIVDEFALRYG+ESI+QAMTHF+CLS+KYLCP VPA MSTLLANINA+YAHTTA+TNV  ++
Sbjct: 3788 AAEKSSKHGADDKANTIIIAMKERMKEREKERPEVFELIRTVFNVGPDTHIDNLEASEQVVLDGTSKWSCKIAVNVVCAQGLIAKDKTGTSDPYVTVQVGKTKKRTKTVPQELNPEWNEKFSFECHNSSDRIKVRVWDEDNDLKARLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYHLEKRSDRSSVSGSIRLQISVEIKGEEQVAPYHVQYTCLHENLFHYLCEKNGGEVSLPDAKGEESWKIYFEEPNEEIVDEFALRYGIESIYQAMTHFNCLSTKYLCPRVPASMSTLLANINAYYAHTTASTNVSASD 4729          

HSP 3 Score: 84.3445 bits (207), Expect = 1.680e-15
Identity = 47/86 (54.65%), Postives = 51/86 (59.30%), Query Frame = 0

Query:    9 EKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL---------------------------KLHVYKKXLQALIYPISS 67
            EK+G  ++ R   DNPFFKDIDSMP EFRPRRKSIPLVSEL                           K+HVYKK LQALIYPISS
Sbjct: 4946 EKSGAAQAGRPGPDNPFFKDIDSMP-EFRPRRKSIPLVSELVLKTMAAKRNASGLSALPRPSLDNEELKMHVYKKTLQALIYPISS 5200          

BLAST of EMLSAG00000007474 vs. L. salmonis peptides
Match: EMLSAP00000007474 (pep:novel supercontig:LSalAtl2s:LSalAtl2s429:306852:321763:-1 gene:EMLSAG00000007474 transcript:EMLSAT00000007474 description:"maker-LSalAtl2s429-snap-gene-2.18")

HSP 1 Score: 2146.32 bits (5560), Expect = 0.000e+0
Identity = 1031/1031 (100.00%), Postives = 1031/1031 (100.00%), Query Frame = 0

Query:    1 MLVKAGLNEKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSELKLHVYKKXLQALIYPISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVILIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQGQ 1031
            MLVKAGLNEKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSELKLHVYKKXLQALIYPISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVILIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQGQ
Sbjct:    1 MLVKAGLNEKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSELKLHVYKKXLQALIYPISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVILIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQGQ 1031          

BLAST of EMLSAG00000007474 vs. L. salmonis peptides
Match: EMLSAP00000007012 (pep:novel supercontig:LSalAtl2s:LSalAtl2s398:388164:498883:-1 gene:EMLSAG00000007012 transcript:EMLSAT00000007012 description:"maker-LSalAtl2s398-augustus-gene-5.6")

HSP 1 Score: 65.855 bits (159), Expect = 4.799e-11
Identity = 38/133 (28.57%), Postives = 67/133 (50.38%), Query Frame = 0

Query:  133 SKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFEC-HNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQIS 264
            + WS  +   ++  + L A D  GTSDPY   ++G  + ++K +   LNP+W E+F      + S  +++ +WD+D            R  DDF+G+  +++ +L+  M+  + L  R D+ S    I L IS
Sbjct:  336 TPWSATVNIVLVEGKNLTAMDFEGTSDPYCKFRLGNDRYKSKPIYLTLNPKWMEQFDLTLFQDQSQELEITIWDKDQ-----------RTKDDFMGKCTVDLSSLA--MEKTHELHLRVDEGSGEIFILLSIS 455          

BLAST of EMLSAG00000007474 vs. SwissProt
Match: gi|160332304|sp|O14795.2|UN13B_HUMAN (RecName: Full=Protein unc-13 homolog B; AltName: Full=Munc13-2; Short=munc13)

HSP 1 Score: 755.362 bits (1949), Expect = 0.000e+0
Identity = 369/657 (56.16%), Postives = 470/657 (71.54%), Query Frame = 0

Query:  387 EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            E+ GP + +L+FW KLI LI S+IEEDKNSY PVLNQFPQELNVG++SA  +W LFA D KYALEEHE+  LCKS+ Y+N HF+VKWLH  YV  +P  +GQVPEYPAWFE FV+QWL+EN+DVSLEFL  A  RDKKD FQ+ +EH+ FS SVVDVFTQL Q FE+++KLEC DP I   YM+RFAKTI KVL  YA+IL  DF  +   E   C+LMNN+QQ R+QLEK + AMGG+EL+ +AA  L +LQ  LN VLDEL+ +F  SF  R+D  V  M  +L +V+G G     +++     AD +L+PLMD LDG+L+++A +C+K+VLKR+LKELW++VM ++E+ IVLPP TD+                        TG    F   +++ S +  +   + +E  R+L+PKQC+VL+ AL+ IKQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTD LIKT+V SQT Q S   +   VGEVS+QVDLFTHPGTGEHKVTVKV+                    ++GPH SDKKR   TKSK+ NW+PKYNE F F++GNE+   SYEL ICVKDYCF R+DR++G++V+ L+D+  +GSCACW  LGR+I MDETG TILRILSQR+NDEVA+EFVKLKSE R
Sbjct:  956 EEQGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLNQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDHLCKSADYMNLHFKVKWLHNEYVRDLPVLQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPSILAHYMRRFAKTIGKVLMQYADILSKDFPAYCTKEKLPCILMNNVQQLRVQLEKMFEAMGGKELDLEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPDARASAAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERMIVLPPLTDQ------------------------TGTQLIFTAAKEL-SHLSKLKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTTQGSGVDD--PVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWAPKYNETFHFLLGNEEGPESYELQICVKDYCFAREDRVLGLAVMPLRDVTAKGSCACWCPLGRKIHMDETGLTILRILSQRSNDEVAREFVKLKSESR 1585          

HSP 2 Score: 516.153 bits (1328), Expect = 4.916e-160
Identity = 238/314 (75.80%), Postives = 277/314 (88.22%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNG-GNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKS KHGA+D+   II+AMK+RMK RER +PEIFE+IR++F+++   H++ M   +Q+VLDGTSKWS KI  TV+CAQGL AKDKTG+SDPYVTVQV K+KKRTKT+   LNP W EKF+FECHNSSDRIKVRVWDED+D+KS+++Q+  RESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYL +  G G V +PEARG+DAWK+YFDE  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  528 LQRAAEKSCKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVNKAAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVSKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVRIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 841          

BLAST of EMLSAG00000007474 vs. SwissProt
Match: gi|296452857|sp|Q9Z1N9.2|UN13B_MOUSE (RecName: Full=Protein unc-13 homolog B; AltName: Full=Munc13-2; Short=munc13)

HSP 1 Score: 753.821 bits (1945), Expect = 0.000e+0
Identity = 367/657 (55.86%), Postives = 472/657 (71.84%), Query Frame = 0

Query:  387 EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            E+ GP + +L+FW KLI LI S+IEEDKNSY PVL+QFPQELNVG++SA  +W LFA D KYALEEHE+ RLCKS+ Y+N HF+VKWLH  YV  +P  +GQVPEYPAWFE FV+QWL+EN+DVSLEFL  A  RDKKD FQ+ +EH+ FS SVVDVFTQL Q FE+++KLEC DP I   YM+RFAKTI KVL  YA+IL  +F  +   E   C+LMNN+QQ R+QLEK + AMGG+EL+ +AA  L +LQ  LN VLDEL+ +F  SF  R+D  V  M  +L +V+G G     +++ V   AD +L+PLMD LDG+L+++A +C+K+VLKR+LKELW++VM ++E+ IVLPP TD++     L    K +++ +K                        +   + +E  R+L+PKQC+VL+ AL+ IKQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTD LIKT+V SQT Q +   +   VGEVS+QVDLFTHPGTGEHKVTVKV+                    ++GPH SDKKR   TKSK+ NW+PKYNE F F++GNE+   +YEL ICVKDYCF R+DR+IG++V+ L+D+  +GSCACW  LGR+I MDETG TILRILSQR+NDEVA+EFVKLKSE R
Sbjct:  967 EEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLSQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRDLPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPNILAHYMRRFAKTIGKVLMQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLTDQTGTQLILTA-AKELSQLSK------------------------LKDHMVREETRNLTPKQCAVLDLALDTIKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQGAGVDD--PVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNNWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESR 1596          

HSP 2 Score: 520.005 bits (1338), Expect = 1.696e-161
Identity = 241/314 (76.75%), Postives = 279/314 (88.85%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNG-GNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+D+   II+AMK+RMK RER +PEIFE+IR++F++S   H++ M   +Q+VLDGTSKWS KI  TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W EKF+FECHNSSDRIKVRVWDED+D+KS+++Q+  RESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYL +  G G V +PEARG+DAWK+YFDE  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  540 LQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPEARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 853          

BLAST of EMLSAG00000007474 vs. SwissProt
Match: gi|51316552|sp|Q62769.2|UN13B_RAT (RecName: Full=Protein unc-13 homolog B; AltName: Full=Munc13-2)

HSP 1 Score: 742.651 bits (1916), Expect = 0.000e+0
Identity = 365/674 (54.15%), Postives = 473/674 (70.18%), Query Frame = 0

Query:  387 EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQ------------DSSSSEEGS-----VGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            E+ GP + +L+FW KLI LI S+IEEDKNSY PVLNQFPQELNVG++SA  +W LFA D KYALEEHE+ RLCKS+ Y+N HF+VKWLH  YV  +P  +GQVPEYPAWFE FV+QWL+EN+DVSLEFL     RDK+D FQ+ +EH+ FS SVVDVFTQL Q FE+++KLEC DP I   YM+RFAKTI KVL  YA+IL  +F  +   E   C+LMNN+QQ R+QLEK + AMGG+EL+ +AA  L +LQ  LN VLDEL+ +F  SF  R+D  V  M  +L +V+G G     +++ V   AD +L+PLMD LDG+L+++A +C+K+VLKR+LKELW++VM ++E+ IVLPP  D++     L    K +++ +K                        +   + +E  R+L+PKQC+VL+ AL+ +KQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTD LIKT+V SQT Q            +    E+G+     VGEVS+QVDLFTHPGTGEHKVTVKV+                    ++GPH SDKKR   TKSK+ +W+PKYNE F F++GNE+   +YEL ICVKDYCF R+DR+IG++V+ L+D+  +GSCACW  LGR+I MDETG TILRILSQR+NDEVA+EFVKLKSE R
Sbjct:  968 EEPGPSIRNLDFWPKLITLIVSIIEEDKNSYTPVLNQFPQELNVGKVSAEVMWHLFAQDMKYALEEHEKDRLCKSADYMNLHFKVKWLHNEYVRELPALQGQVPEYPAWFEQFVLQWLDENEDVSLEFLRGGLERDKRDGFQQTSEHALFSCSVVDVFTQLNQSFEIIRKLECPDPSILAHYMRRFAKTIGKVLIQYADILSKNFPAYCTKERLPCILMNNMQQLRVQLEKMFEAMGGKELDSEAADSLKELQVKLNTVLDELSMVFGNSFQVRIDECVRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVLKELWRVVMNTMERVIVLPPLIDQTGTQLILTA-AKELSQLSK------------------------LKDHMVREETRNLTPKQCAVLDLALDTVKQYFHAGGNGLKKTFLEKSPDLQSLRYALSLYTQTTDTLIKTFVRSQTAQVHDGKGIRFTANEDIRPEKGAGVDDPVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWQTAGMFRPFVEVTMVGPHQSDKKRKFTTKSKSNSWTPKYNETFHFLLGNEEGPEAYELQICVKDYCFAREDRVIGLAVMPLRDVAAKGSCACWCPLGRKIHMDETGMTILRILSQRSNDEVAREFVKLKSESR 1616          

HSP 2 Score: 517.309 bits (1331), Expect = 3.080e-160
Identity = 239/314 (76.11%), Postives = 279/314 (88.85%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNG-GNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+D+   II+AMK+RMK RER +PEIFE+IR++F++S   H++ M   +Q+VLDGTSKWS KI  TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W EKF+FECHNSSDRIKVRVWDED+D+KS+++Q+  RESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRLQI+VEIKGEEKVAPYHVQYTCLHENLFHYL +  G G V +P+ARG+DAWK+YFDE  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  541 LQRAAEKSSKHGAEDRTQNIIMAMKDRMKIRERNKPEIFEVIRDVFTVSKVAHVQQMKTVKQSVLDGTSKWSAKITITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIFGNLNPVWEEKFHFECHNSSDRIKVRVWDEDDDIKSRVKQRLKRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLQINVEIKGEEKVAPYHVQYTCLHENLFHYLTDIQGSGGVWIPDARGDDAWKVYFDETAQEIVDEFAMRYGIESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 854          

BLAST of EMLSAG00000007474 vs. SwissProt
Match: gi|342187113|sp|Q4KUS2.3|UN13A_MOUSE (RecName: Full=Protein unc-13 homolog A; AltName: Full=Munc13-1)

HSP 1 Score: 735.332 bits (1897), Expect = 0.000e+0
Identity = 369/705 (52.34%), Postives = 488/705 (69.22%), Query Frame = 0

Query:  343 CLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHV--KDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAE-KTKKVEDVTGFFKSFGGKQDVKSSVMSVVSD-ISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            CL+S Y           +  N +  Y     T      E     E+ GP + +L+FW KLI LI S+IEEDKNSY P LNQFPQELNVG++SA  +W LFA D KYA+EEH++ RLCKS+ Y+N HF+VKWL+  YV+ +P +K +VPEYPAWFEPFV+QWL+EN++VS +FLH A  RDKKD FQ+ +EH+ FS SVVDVF+QL Q FE+++KLEC DP+I   YM+RFAKTI  VL  YA+I+  DF ++   + E   C+LMNN QQ R+QLEK + AMGG+EL+ +A+  L +LQ  LN VLDEL+H+FA SF   ++  V  MG +L +VKG G   A + S V   AD +LQP+MDLLD +L+++A+IC+K+VLKR+LKELWK+VM ++EK IVLPP TD++     L    K + + + K      G    F   +++    +S + D + +E  +SL+PKQC+V+E AL+ IKQYFHAGG+GLK +++ KS +L+SL++ALSLYTQ TD LIKT+V +Q+ Q   S  E  VGEVSV V+LFTHPGTGE KVTVKV+                    ++GP LSDKKR   TKSKN +W+PKYNE F F +  +     YEL +CVKDYCF R+DR +G++V+QL+++  +GS ACWL LGRRI MD+TG T+LRILSQR+NDEVAKEFVKLKS+ R
Sbjct: 1012 CLNSTY---------EYIFNNCHELYGREYQTDPAKKGEVP--PEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQ--LSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQ--GSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTR 1701          

HSP 2 Score: 508.064 bits (1307), Expect = 2.711e-156
Identity = 233/314 (74.20%), Postives = 277/314 (88.22%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCE-KNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+D+   II+ +K+RMK RER +PEIFELI+ IF+++   H + M   +Q+VLDGTSKWS KI+ TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W E F+FECHNSSDRIKVRVWDED+D+KS+++Q+F RESDDFLGQTIIEVRTLSGEMDVWYNL+KR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFH++ + +N G V +P+A+G+DAWK+Y+DE  QEIVDEFA+RYGVESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  613 LQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 926          

BLAST of EMLSAG00000007474 vs. SwissProt
Match: gi|374095515|sp|Q9UPW8.4|UN13A_HUMAN (RecName: Full=Protein unc-13 homolog A; AltName: Full=Munc13-1)

HSP 1 Score: 735.332 bits (1897), Expect = 0.000e+0
Identity = 363/668 (54.34%), Postives = 477/668 (71.41%), Query Frame = 0

Query:  387 EDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHV--KDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNL---------AGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            E+ GP + +L+FW KLI LI S+IEEDKNSY P LNQFPQELNVG++SA  +W LFA D KYA+EEH++ RLCKS+ Y+N HF+VKWL+  YV+ +P +K +VPEYPAWFEPFV+QWL+EN++VS +FLH A  RDKKD FQ+ +EH+ FS SVVDVF+QL Q FE+++KLEC DP+I   YM+RFAKTI  VL  YA+I+  DF ++   + E   C+LMNN QQ R+QLEK + AMGG+EL+ +A+ +L +LQ  LN VLDEL+ +FA SF   ++  V  MG +L +VKG G   A + S V   AD +LQP+MDLLD +L+++A+IC+K+VLKR+LKELWK+VM ++EK IVLPP TD++  I NL          G VK +   +   + +    K  G    +K  ++       +E  +SL+PKQC+V+E AL+ IKQYFHAGG+GLK +++ KS +L+SL++ALSLYTQ TD LIKT+V +Q+ Q      E  VGEVSV V+LFTHPGTGEHKVTVKV+                    +IGP LSDKKR   TKSKN +W+PKYNE F F +  +     YEL +CVKDYCF R+DR +G++V+QL+++  +GS ACWL LGRRI MD+TG T+LRILSQR+NDEVAKEFVKLKS+ R
Sbjct: 1036 EEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWNLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVTELPAFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIISKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASDILKELQVKLNNVLDELSRVFATSFQPHIEECVKQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQT-MIGNLLRKHGKGLEKGRVK-LPSHSDGTQMIFNAAKELGQLSKLKDHMV-------REEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQ--GLGVEDPVGEVSVHVELFTHPGTGEHKVTVKVVAANDLKWQTSGIFRPFIEVNIIGPQLSDKKRKFATKSKNNSWAPKYNESFQFTLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTR 1692          

HSP 2 Score: 507.294 bits (1305), Expect = 4.261e-156
Identity = 233/314 (74.20%), Postives = 277/314 (88.22%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCE-KNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+D+   II+ +K+RMK RER +PEIFELI+ IF+++   H + M   +Q+VLDGTSKWS KI+ TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W E F+FECHNSSDRIKVRVWDED+D+KS+++Q+F RESDDFLGQTIIEVRTLSGEMDVWYNL+KR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFH++ + +N G V +P+A+G+DAWK+Y+DE  QEIVDEFA+RYGVESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  604 LQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKTAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 917          

BLAST of EMLSAG00000007474 vs. SwissProt
Match: gi|51316551|sp|Q62768.1|UN13A_RAT (RecName: Full=Protein unc-13 homolog A; AltName: Full=Munc13-1)

HSP 1 Score: 725.317 bits (1871), Expect = 0.000e+0
Identity = 367/722 (50.83%), Postives = 488/722 (67.59%), Query Frame = 0

Query:  343 CLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHV--KDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAE-KTKKVEDVTGFFKSFGGKQDVKSSVMSVVSD-ISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQ-----------------DSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            CL+S Y           +  N +  Y     T      E     E+ GP + +L+FW KLI LI S+IEEDKNSY P LNQFPQELNVG++SA  +W LFA D KYA+EEH++ RLCKS+ Y+N HF+VKWL+  YV+ +P +K +VPEYPAWFEPFV+QWL+EN++VS +FLH A  RDKKD FQ+ +EH+ FS SVVDVF+QL Q FE+++KLEC DP+I   YM+RFAKTI  VL  YA+I+  DF ++   + E   C+LMNN QQ R+QLEK + AMGG+EL+ +A+  L +LQ  LN VLDEL+H+FA SF   ++  V  MG +L +VKG G   A + S V   AD +LQP+MDLLD +L+++A+IC+K+VLKR+LKELWK+VM ++E+ IVLPP TD++     L    K + + + K      G    F   +++    +S + D + +E  +SL+PKQC+V+E AL+ IKQYFHAGG+GLK +++ KS +L+SL++ALSLYTQ TD LIKT+V +Q+ Q                 +  S  E  VGEVSV V+LFTHPGTGE KVTVKV+                    ++GP LSDKKR   TKSKN +W+PKYNE F F +  +     YEL +CVKDYCF R+DR + ++V+QL+++  +GS ACWL LGRRI MD+TG T+LRILSQR+NDEVAKEFVKLKS+ R
Sbjct: 1016 CLNSTY---------EYIFNNCHELYGREYQTDPAKKGEVP--PEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMERTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQ--LSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVELAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTR 1724          

HSP 2 Score: 502.671 bits (1293), Expect = 3.893e-154
Identity = 231/314 (73.57%), Postives = 276/314 (87.90%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCE-KNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+D+   II+ +K+RMK RER +PEIFELI+ +F+++   H + M   +Q+VLDGTSKWS KI+ TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W E F+FECHNSSDRIKVRV DED+D+KS+++Q+F RESDDFLGQTIIEVRTLSGEMDVWYNL+KR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFH++ + +N G V +P+A+G+DAWK+Y+DE  QEIVDEFA+RYGVESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV
Sbjct:  617 LQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEVFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVLDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNV 930          

BLAST of EMLSAG00000007474 vs. SwissProt
Match: gi|152031726|sp|Q8K0T7.3|UN13C_MOUSE (RecName: Full=Protein unc-13 homolog C; AltName: Full=Munc13-3)

HSP 1 Score: 692.96 bits (1787), Expect = 0.000e+0
Identity = 340/670 (50.75%), Postives = 463/670 (69.10%), Query Frame = 0

Query:  377 VDPNEAKRD--SEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRG 1024
            +DP++ K+D   ED GP   +L+FW +LI L+ ++I+EDK +Y PVLNQFPQELN+G++SA  +W LFA+D KYALEEHE+ RLCKS+ Y+N HF+VKW +  YV  +P +K  VPEY  WFEPFVMQWL+EN+DVS+EFLH A  RDKKD FQ+ ++H+ FS SVVDVF QL Q FE+++KLEC +PE     M+RFAKTI KVL  YA I+  DF ++   E   C+LMNNIQQ R+QLEK + +MGG+EL+ +A+++L +LQ  LNGVLD L+  +  SF   ++  +  MG  L +++  G   A +K+     A+ +L+PLMD LD +LS+ A+IC+K+VLKR+LKELWK+V+  +EK IVLPP TD+                        TG    F   +D+   +  +   + +E  + L+P+QC+++E  L  IKQYFHAGG GLK +++ KS +L SL++ALSLYTQTTDALIK ++ +Q  Q  SS +  +VG++SV VD+ T PGTG+HKVTVKVI                    ++GP L DKKR Q TK+K+  WSPKYNE F FI+GNE++ G+YELH+ VKDYCF R+DR+IG++V+QL++I ++GS   W  L + + MDETG TILRILSQRT+D+VAKEFV+LKSE R 
Sbjct: 1565 IDPSK-KQDVPREDQGPTTKNLDFWPQLITLMVTIIDEDKTAYTPVLNQFPQELNMGKISAEIMWSLFALDMKYALEEHEKQRLCKSTDYMNLHFKVKWFYNEYVRELPAFKDAVPEYSLWFEPFVMQWLDENEDVSMEFLHGALGRDKKDGFQQTSDHALFSCSVVDVFAQLNQSFEIIKKLECPNPEALSHLMRRFAKTINKVLVQYAAIVSNDFSSYCDKETVPCILMNNIQQLRVQLEKMFESMGGKELDPEASTILKELQIKLNGVLDALSITYGESFQLTIEECIKQMGAELNQMRANGNSTA-NKNSAAMDAEIVLRPLMDFLDKTLSLSAKICEKTVLKRVLKELWKLVLNKIEKQIVLPPLTDQ------------------------TGPQMIFIAAKDL-GQLSKLKEHMIREDAKGLTPRQCAIVEVVLATIKQYFHAGGNGLKKNFLEKSPDLHSLRYALSLYTQTTDALIKKFIETQGSQSRSSKD--AVGQISVHVDVTTTPGTGDHKVTVKVIAINDLNWQTTAMFRPFVEVCMLGPSLGDKKRKQGTKTKSNTWSPKYNETFQFILGNENRPGAYELHLSVKDYCFAREDRIIGMTVIQLQNIAEKGSYGAWYPLLKNLSMDETGLTILRILSQRTSDDVAKEFVRLKSETRS 2205          

HSP 2 Score: 508.064 bits (1307), Expect = 5.233e-154
Identity = 232/321 (72.27%), Postives = 279/321 (86.92%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCE-KNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNEAKR 384
            +  AAEKSSKHGA+DK  TII AMKERMK RER RPE+FE+I+ +F IS ++ ++     +Q+VLDGTSKWS KI  TV+ AQGL AKDKTG+SDPYVTVQVGK+K+RTKT+   LNP W+EKF+FECHNS+DRIKVRVWDED+D+KS+++Q F +ESDDFLGQTI+EVRTLSGEMDVWYNLEKR+DKS+VSGAIRL+I+VEIKGEEKVAPYH+QYTCLHENLFHYL E K+ G+V +PE +G++AWK++FD+  QEIVDEFA+RYGVESI+QAMTHF CLSSKY+CPGVPA MS LLANINAFYAHTT +TNV  + + R
Sbjct: 1144 LQRAAEKSSKHGAEDKTQTIITAMKERMKIRERNRPEVFEVIQEMFQISKEDFVQYTKAAKQSVLDGTSKWSAKITITVVSAQGLQAKDKTGSSDPYVTVQVGKNKRRTKTIFGNLNPVWDEKFFFECHNSTDRIKVRVWDEDDDIKSRVKQHFKKESDDFLGQTIVEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLKINVEIKGEEKVAPYHIQYTCLHENLFHYLTEVKSNGSVKIPEVKGDEAWKVFFDDASQEIVDEFAMRYGVESIYQAMTHFSCLSSKYMCPGVPAVMSALLANINAFYAHTTVSTNVQVSASDR 1464          

BLAST of EMLSAG00000007474 vs. SwissProt
Match: gi|148887448|sp|Q8NB66.3|UN13C_HUMAN (RecName: Full=Protein unc-13 homolog C; AltName: Full=Munc13-3)

HSP 1 Score: 689.493 bits (1778), Expect = 0.000e+0
Identity = 346/704 (49.15%), Postives = 473/704 (67.19%), Query Frame = 0

Query:  343 CLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNEAKRD--SEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRG 1024
            CL S Y           +  N +  Y+  T     DP++ K+D   ED GP   +L+FW +LI L+ ++I+EDK +Y PVLNQFPQELN+G++SA  +W LFA+D KYALEEHE  RLCKS+ Y+N HF+VKW +  YV  +P +K  VPEY  WFEPFVMQWL+EN+DVS+EFLH A  RDKKD FQ+ +EH+ FS SVVDVF QL Q FE+++KLEC +PE     M+RFAKTI KVL  YA I+  DF +H   E   C+LMNNIQQ R+QLEK + +MGG+EL+ +A+++L +LQ  L+GVLDEL+  +  SF   ++  +  M   L +++  G   + +K+     A+ +L+ LMD LD +LS+ A+IC+K+VLKR+LKELWK+V+  +EK IVLPP TD++   + +    K++ + +K  E +                         +E  R L+P+QC+++E  L  IKQYFHAGG GLK +++ KS +L+SL++ALSLYTQTTDALIK ++ +QT Q  SS +  +VG++SV VD+   PGTG+HKVTVKVI                    ++GP+L DKKR Q TK+K+  WSPKYNE F FI+G E++ G+YELH+ VKDYCF R+DR+IG++V+QL++I ++GS   W  L + I MDETG TILRILSQRT+D+VAKEFV+LKSE R 
Sbjct: 1549 CLDSTY---------KYIFDNCHELYSQLT-----DPSK-KQDIPREDQGPTTKNLDFWPQLITLMVTIIDEDKTAYTPVLNQFPQELNMGKISAEIMWTLFALDMKYALEEHENQRLCKSTDYMNLHFKVKWFYNEYVRELPAFKDAVPEYSLWFEPFVMQWLDENEDVSMEFLHGALGRDKKDGFQQTSEHALFSCSVVDVFAQLNQSFEIIKKLECPNPEALSHLMRRFAKTINKVLLQYAAIVSSDFSSHCDKENVPCILMNNIQQLRVQLEKMFESMGGKELDSEASTILKELQVKLSGVLDELSVTYGESFQVIIEECIKQMSFELNQMRANGNTTS-NKNSAAMDAEIVLRSLMDFLDKTLSLSAKICEKTVLKRVLKELWKLVLNKIEKQIVLPPLTDQTGP-QMIFIAAKDLGQLSKLKEHMI------------------------REDARGLTPRQCAIMEVVLATIKQYFHAGGNGLKKNFLEKSPDLQSLRYALSLYTQTTDALIKKFIDTQTSQSRSSKD--AVGQISVHVDITATPGTGDHKVTVKVIAINDLNWQTTAMFRPFVEVCILGPNLGDKKRKQGTKTKSNTWSPKYNETFQFILGKENRPGAYELHLSVKDYCFAREDRIIGMTVIQLQNIAEKGSYGAWYPLLKNISMDETGLTILRILSQRTSDDVAKEFVRLKSETRS 2209          

HSP 2 Score: 509.99 bits (1312), Expect = 9.705e-155
Identity = 231/321 (71.96%), Postives = 279/321 (86.92%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCE-KNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNEAKR 384
            +  AAEKSSKHGA+DK  TII AMKERMK RE+ RPE+FE+I+ +F IS ++ ++     +Q+VLDGTSKWS KI  TV+ AQGL AKDKTG+SDPYVTVQVGK+K+RTKT+   LNP W+EKFYFECHNS+DRIKVRVWDED+D+KS+++Q F +ESDDFLGQTI+EVRTLSGEMDVWYNLEKR+DKS+VSGAIRL+I+VEIKGEEKVAPYH+QYTCLHENLFHYL E K+ G V +PE +G++AWK++FD+  QEIVDEFA+RYG+ESI+QAMTHF CLSSKY+CPGVPA MSTLLANINAFYAHTT +TN+  + + R
Sbjct: 1148 LQRAAEKSSKHGAEDKTQTIITAMKERMKIREKNRPEVFEVIQEMFQISKEDFVQFTKAAKQSVLDGTSKWSAKITITVVSAQGLQAKDKTGSSDPYVTVQVGKNKRRTKTIFGNLNPVWDEKFYFECHNSTDRIKVRVWDEDDDIKSRVKQHFKKESDDFLGQTIVEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLKINVEIKGEEKVAPYHIQYTCLHENLFHYLTEVKSNGGVKIPEVKGDEAWKVFFDDASQEIVDEFAMRYGIESIYQAMTHFSCLSSKYMCPGVPAVMSTLLANINAFYAHTTVSTNIQVSASDR 1468          

BLAST of EMLSAG00000007474 vs. SwissProt
Match: gi|51316553|sp|Q62770.3|UN13C_RAT (RecName: Full=Protein unc-13 homolog C; AltName: Full=Munc13-3)

HSP 1 Score: 679.863 bits (1753), Expect = 0.000e+0
Identity = 340/679 (50.07%), Postives = 465/679 (68.48%), Query Frame = 0

Query:  370 HTTATTNVDPNEAKRD--SEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGED 1026
            H   +  +DP++ K+D   E+ GP   +L+FW +LI L+ ++I+EDK +Y PVLNQFPQELN+G++SA  +W LFA+D KYALEEHE+ RLCKS+ Y+N HF+VKW +  YV  +P +K  VPEY  WFEPFVMQWL+EN+DVS+EFLH A  RDKKD FQ+ ++H+ FS SVVDVF QL Q FE+++KLEC +PE     M+RFAKTI KVL  YA I+  DF ++   E   C+LMNNIQQ R+QLEK + +MGG+EL+ +A+++L +LQ  LNGVLD L+  +  SF   ++  +  MG  L +++  G   A +K+     A+ +L+PLMD LD  LS+ A+IC+K+VLKR+LKELWK+V+  +EK IVLPP TD++                    + +    K  G    +K  ++       ++  + L+P+QC+++E  L  IKQYFHAGG GLK +++ KS +L SL++ALSLYTQTTDALIK ++ +Q  Q  SS +  +VG++SV VD+ T PGTGEHKVTVKVI                    ++GP L DKKR Q TK+K+  WSPKYNE F FI+GNE++ G+YELH+ VKDYCF R+DR+IG++V+QL++I ++GS   W  L + + MDETG TILRILSQRT+D+VAKEFV+LKSE R  D
Sbjct: 1552 HELYSQLIDPSK-KQDVPREEQGPTTKNLDFWPQLITLMVTIIDEDKTAYTPVLNQFPQELNMGKISAEIMWSLFALDMKYALEEHEKQRLCKSTDYMNLHFKVKWFYNEYVRELPAFKDAVPEYSLWFEPFVMQWLDENEDVSMEFLHGALGRDKKDGFQQTSDHALFSCSVVDVFAQLNQSFEIIKKLECPNPEALSHLMRRFAKTINKVLVQYAAIVSSDFSSYCDKETVPCILMNNIQQLRVQLEKMFESMGGKELDPEASTILKELQIKLNGVLDALSVTYGESFQLVIEECIKQMGAELNQMRANGN-SAANKNNAAMDAEIVLRPLMDFLDKILSLSAKICEKTVLKRVLKELWKLVLNKIEKQIVLPPLTDQTGP------------------QMIFIAAKELGQLSKLKEHMI-------RDDAKGLTPRQCAIMEVVLATIKQYFHAGGNGLKKNFLEKSPDLHSLRYALSLYTQTTDALIKKFIETQGSQSRSSKD--AVGQISVHVDVTTTPGTGEHKVTVKVIAINDLNWQTTTMFRPFVEVCMLGPSLGDKKRKQGTKTKSNTWSPKYNETFQFILGNENRPGAYELHLSVKDYCFAREDRIIGMTVIQLQNIAEKGSYGAWYPLLKNLSMDETGLTILRILSQRTSDDVAKEFVRLKSETRSID 2201          

HSP 2 Score: 508.834 bits (1309), Expect = 2.038e-154
Identity = 233/321 (72.59%), Postives = 279/321 (86.92%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCE-KNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNEAKR 384
            +  AAEKSSKHGA+DK  TII AMKERMK RER RPE+FE+I+ +F IS ++ ++     +Q+VLDGTSKWS KI  TV+ AQGL AKDKTG+SDPYVTVQVGK+K+RTKT+   LNP W+EKFYFECHNS+DRIKVRVWDED+D+KS+++Q F +ESDDFLGQTI+EVRTLSGEMDVWYNLEKR+DKS+VSGAIRL+I+VEIKGEEKVAPYH+QYTCLHENLFHYL E K+ G+V +PE +G++AWK++FD+  QEIVDEFA+RYGVESI+QAMTHF CLSSKY+CPGVPA MS LLANINAFYAHTT +TNV  + + R
Sbjct: 1138 LQRAAEKSSKHGAEDKTQTIITAMKERMKIRERNRPEVFEVIQEMFQISKEDFVQYTKAAKQSVLDGTSKWSAKITITVVSAQGLQAKDKTGSSDPYVTVQVGKNKRRTKTIFGNLNPVWDEKFYFECHNSTDRIKVRVWDEDDDIKSRVKQHFKKESDDFLGQTIVEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLKINVEIKGEEKVAPYHIQYTCLHENLFHYLTEVKSNGSVKIPEVKGDEAWKVFFDDASQEIVDEFAMRYGVESIYQAMTHFSCLSSKYMCPGVPAVMSALLANINAFYAHTTVSTNVQVSASDR 1458          

BLAST of EMLSAG00000007474 vs. SwissProt
Match: gi|51338690|sp|P27715.4|UNC13_CAEEL (RecName: Full=Phorbol ester/diacylglycerol-binding protein unc-13; AltName: Full=Uncoordinated protein 13)

HSP 1 Score: 662.914 bits (1709), Expect = 0.000e+0
Identity = 336/661 (50.83%), Postives = 443/661 (67.02%), Query Frame = 0

Query:  391 PRLD-DLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFF----KSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQ-GSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGE 1025
            P LD  ++FW++ I  I  VIEED+ +Y P LNQFPQELNVG LSA T+W ++  D K ALEEH Q + CK+  Y+N +F+VK  +  YV+ +  YK  +PE+PAWF PFVM WLNEND+ S++ L +AY  DK D F + +EH+ FSNSVVDVFTQL    ++L++++C +PE+    MKRF+KT+ KVL AYA++++ DF     DE  AC+LMNN+QQ R+QLEK Y  MGG EL+E    VL  LQ  LN VLD L+  F  +    +    I +G LL ++KG      + K++VQ  AD +L+PLMDLL+GSL  YA+ C+K+VLK +LKELWKI + ++EK +VLPP +DK A +K L            K+ DVT       +S  G   VK      + D+++E E+SL+P+QC+VL+ AL+ IK  FHA G GLK S+  KS EL+SL++ALSLYTQTT+ LIKT+++SQ  QD  S E+  VGEVSVQVDLF+HPGTGE KVTVK++                    L+GPHLSDKKR   TK+K  NW+PK+NE F F +GNE +   YEL   VKDYCF RDDR++GV V+QL  + DQ GSCA W+ LG R+ +DETG  +LRILSQR  DEVAK+FV+LK+E R E
Sbjct: 1498 PILDTSVQFWYEFIDYIMRVIEEDQKNYTPALNQFPQELNVGNLSAETLWSMYKNDLKMALEEHAQKKRCKTPEYMNLYFKVKGFYFKYVADLSTYKSSIPEFPAWFIPFVMDWLNENDEHSMDILRNAYNVDKADNFPQTSEHTKFSNSVVDVFTQLNAALKLLKQMDCPNPEVAADMMKRFSKTLNKVLLAYADMVQKDFPKFAHDEKLACILMNNVQQLRVQLEKIYETMGGAELDEHIGQVLTVLQKKLNSVLDRLSAEFVTTLEPHIHEQTIKLGMLLVKIKGP----QLQKTQVQPEADAVLEPLMDLLEGSLRRYADQCEKTVLKYILKELWKITIVNMEKRVVLPPLSDK-ALLKQLPN---------AKIGDVTKLMSTNIQSIKGMNSVKD-----MMDMARESEKSLTPRQCTVLDCALDAIKDSFHASGKGLKKSFFEKSPELQSLKYALSLYTQTTEQLIKTFITSQRQQDLPSQEQ-PVGEVSVQVDLFSHPGTGEQKVTVKILAANDLRWQTSSAFKPFVEVHLVGPHLSDKKRKWSTKTKAGNWAPKFNETFHFFLGNEGEPEHYELMFQVKDYCFARDDRVVGVGVLQLSSVVDQAGSCAMWVQLGTRLHIDETGLILLRILSQRQTDEVAKDFVRLKTECRYE 2138          

HSP 2 Score: 463.766 bits (1192), Expect = 1.227e-138
Identity = 211/313 (67.41%), Postives = 261/313 (83.39%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKS+KHG  D+  +++  +++RMK +E+ +PE+F++IR +F +  +   E +   + ++L+G+SKWS KI  TV+CAQGLIAKDKTG SDPYVT QVGK+K+RT+T+ QELNP WNEKF+FECHNS+DRIKVRVWDEDNDLKSKLRQK TRESDDFLGQT+IEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL I+VEIKGEEK+APYHVQYTCLHE+LF   C      V LP+ RGED+WK+ F E  QEI +EFA+RYG+ESI+QAMTHF CL ++Y+C GVPA +STLLANINA+YAHTTAT+ V
Sbjct: 1085 LQRAAEKSTKHGEADRTQSLVNVIRDRMKIQEQNKPEVFQMIRTVFDVDENIQKETLKTVKASILEGSSKWSAKITLTVLCAQGLIAKDKTGKSDPYVTAQVGKTKRRTRTIHQELNPVWNEKFHFECHNSTDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTVIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHINVEIKGEEKLAPYHVQYTCLHEHLFAAHCVDE--EVKLPKVRGEDSWKVCFQETGQEIAEEFAMRYGIESIYQAMTHFACLCTRYMCAGVPAVLSTLLANINAYYAHTTATSAV 1395          

BLAST of EMLSAG00000007474 vs. Select Arthropod Genomes
Match: EAA06229.5 (AGAP000065-PA [Anopheles gambiae str. PEST])

HSP 1 Score: 1006.13 bits (2600), Expect = 0.000e+0
Identity = 523/940 (55.64%), Postives = 662/940 (70.43%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ----GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQ-THQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQ 977
             + A+EKSSKHGA+DKAN++I AMKERMK+RER+RPEIFELIR +F++   +H   M   +Q+VLDGTSKWS KIA TVICAQGLIAKDK+GTSDPYVTVQV K KKRT+T+PQELNP WNEKF FECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHEN+FHYLCE+  G V+LP+ +G+D WKIYFDE  +EIVDEFA+RYG+E+I+QAMTHFHCLS+KYLC GVPA MSTLLANINA+YAHTTA++ V  ++ +  + + G      E + KL+  + + +  D + Y    N FP       +   +   L      + ++  E S   ++ST +    +   L  TY                       Q+ +E         H+ ++    D F + T+         DV ++L          +C DPEIWKRYM+RFAKTIVKVL AY +I+K +F + + +E TAC+LMNNIQQ R+QLEK + +MGG +LEEDAA++L +LQ  LN  LD+LA  FA S   R+  SV  +G LL +VK          A   + +   AD +L+PLMDLLDGSLSMYA+ C+K+VLKRLLKELWKIV+++LEK IVLPP TD++   K+L  + KN+A    K+ED++   K+   GKQD K +V+S V DISKE E++LSPKQC+VLE AL  I+QYFHAGG GLKM ++ KS EL+SL++ALSLYTQTTD LIK++VS Q   ++  S ++ SVGEVS+Q+D+ ++P  GE K++VKVI                     LIGPHL+D+KR   TKSK+ NWSPKYN+ F FIIGNE+Q+  +ELHICVKDYCF R+DRL+GV++++LKD+ DQ
Sbjct: 1386 FAGASEKSSKHGAEDKANSVIAAMKERMKQRERDRPEIFELIRKVFAVEEKSHAGHMKAVKQSVLDGTSKWSAKIAITVICAQGLIAKDKSGTSDPYVTVQVSKVKKRTRTMPQELNPVWNEKFNFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENVFHYLCEEALGAVNLPQTKGDDGWKIYFDETPEEIVDEFAMRYGIENIYQAMTHFHCLSTKYLCAGVPAVMSTLLANINAYYAHTTASSAVSASD-RFAASNFGK-----EKFVKLLDQLHNSLRIDLSMY---RNNFPASSQEKLMDLKSTVDLLTSITFFRMKVQELSSPPRASTVVKDCVKA-CLRSTY-----------------------QFFSE---------HALFSNSVVDVFTQLTQ-------CFDVVSKL----------DCPDPEIWKRYMRRFAKTIVKVLLAYVDIVKSEFPDIMHEERTACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNTALDDLATQFAKSLEPRITGSVRELGDLLQQVKQQSSLFHAPPADDANLIAIEADEVLRPLMDLLDGSLSMYAQSCEKTVLKRLLKELWKIVIRTLEKTIVLPPMTDRTMVFKHLTDNAKNLAANA-KIEDMSRLIKNHMSGKQDAK-NVLSGVMDISKEMEKNLSPKQCAVLEVALGTIQQYFHAGGNGLKMPFLEKSPELQSLKYALSLYTQTTDTLIKSFVSGQGASEEGVSHDDASVGEVSIQIDVSSNPENGEQKISVKVIAANDLKWTVPSGMFRPFIEVNLIGPHLNDRKRKFATKSKSNNWSPKYNDTFHFIIGNEEQLEFFELHICVKDYCFAREDRLVGVAILKLKDVVDQ 2264          

HSP 2 Score: 66.6254 bits (161), Expect = 5.025e-10
Identity = 38/76 (50.00%), Postives = 42/76 (55.26%), Query Frame = 0

Query:   17 NRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL-------------------------KLHVYKKXLQALIYPISS 67
            N    DN F+ +IDSMP + RPRRKSIPLVSEL                         K+HVYKK LQALIYPISS
Sbjct: 1257 NNGLGDNTFYSNIDSMP-DIRPRRKSIPLVSELTMAATKRNAGLTSAVPRATLNDEELKMHVYKKALQALIYPISS 1331          

BLAST of EMLSAG00000007474 vs. Select Arthropod Genomes
Match: gb|KXZ75744.1| (Protein unc-13 homolog B-like Protein [Tribolium castaneum])

HSP 1 Score: 978.393 bits (2528), Expect = 0.000e+0
Identity = 469/679 (69.07%), Postives = 555/679 (81.74%), Query Frame = 0

Query:  378 DPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ---GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQGQ 1031
            DPNE KRD ED GPRLD ++FWHKLIALI SVIEEDKNSYGPVLNQFPQELN+GQLSAAT+W LFAVD KYALEEHEQ RLCKSS Y+N HF+VKWL+  YV  VPPYKG VPEYPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQ+++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYMKRFAKTIVKVL AYA+I+KG+F  HVK+E  AC+LMNNIQQ R+QLEK + +MGG++LEEDAA++L  LQ TLNG LDELA  FA S   R+  SV  +G LL  +KGGGQ    Q   ++ V + AD +L+PLMDLLDGSLS+YA+ C+K+VLKRLLKELWKIVM+ LEK +VLPP TDK+   K+L  + KN+A KT K+ED++  FK+   GKQDVK++ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK ++++KS EL+SL++ALSLYTQTTD LIKT+V++Q ++D+   +EGSVGEVS+QVDLFTHPGTGEHKVTVKV+                     LIGPHLSDKKR   TKSK  NWSPKYNE F F IGNE+Q+  +ELHICVKDYCF R+DRL+GV+V+QLKDI ++GSCACWLSLG+RIQMDETGWTILRILSQRTNDEVAKEFVKLKS++R E+PL  Q
Sbjct:  598 DPNEPKRDPEDHGPRLDSVDFWHKLIALIVSVIEEDKNSYGPVLNQFPQELNIGQLSAATMWSLFAVDMKYALEEHEQHRLCKSSAYMNLHFKVKWLYSNYVKDVPPYKGAVPEYPAWFEPFVMQWLNENDDVSLEYLHGAFNRDKKDGFQRSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMKRFAKTIVKVLIAYADIVKGEFPEHVKEERIACILMNNIQQLRVQLEKMFESMGGEKLEEDAANILKDLQQTLNGCLDELATQFASSLEPRITVSVKELGDLLLSIKGGGQPGTTQPAQRNVVASEADEVLRPLMDLLDGSLSLYAQSCEKTVLKRLLKELWKIVMRILEKTVVLPPMTDKTMMFKSLTDNAKNLAAKT-KIEDMSTLFKNHMAGKQDVKNA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKAFLDKSPELQSLRYALSLYTQTTDTLIKTFVTTQINEDTLDGQEGSVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWNVVSGMFRPFVEVNLIGPHLSDKKRKHATKSKANNWSPKYNETFHFTIGNEEQLDYFELHICVKDYCFAREDRLVGVAVMQLKDIVEKGSCACWLSLGKRIQMDETGWTILRILSQRTNDEVAKEFVKLKSDIRQENPLPNQ 1274          

HSP 2 Score: 490.73 bits (1262), Expect = 1.820e-153
Identity = 223/275 (81.09%), Postives = 250/275 (90.91%), Query Frame = 0

Query:  107 RNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            R  FS+ PD HI+ + Q EQ  ++GTSKWSCKIA TV  AQGLIAKDK+GTSDPYVTVQVGK KKRT+T+PQELNP W+EKFYFECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G VSLP+A+G+DAWK+YF+E  +EIVDEFA+RYG+ESI+QAMTHFHCLS+KYLCPGVPA MS LLANINA+YAHTTA++ V  ++
Sbjct:  219 RTTFSVDPDTHIDSIEQAEQMTIEGTSKWSCKIAITVKSAQGLIAKDKSGTSDPYVTVQVGKVKKRTRTMPQELNPVWDEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEQNSGVVSLPQAKGDDAWKVYFEEAGEEIVDEFAMRYGIESIYQAMTHFHCLSTKYLCPGVPAVMSMLLANINAYYAHTTASSAVSASD 493          

BLAST of EMLSAG00000007474 vs. Select Arthropod Genomes
Match: gb|KXZ75745.1| (Protein unc-13 homolog B-like Protein [Tribolium castaneum])

HSP 1 Score: 961.059 bits (2483), Expect = 0.000e+0
Identity = 470/721 (65.19%), Postives = 555/721 (76.98%), Query Frame = 0

Query:  378 DPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ---GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQ------------------------------------------THQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQGQ 1031
            DPNE KRD ED GPRLD ++FWHKLIALI SVIEEDKNSYGPVLNQFPQELN+GQLSAAT+W LFAVD KYALEEHEQ RLCKSS Y+N HF+VKWL+  YV  VPPYKG VPEYPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQ+++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYMKRFAKTIVKVL AYA+I+KG+F  HVK+E  AC+LMNNIQQ R+QLEK + +MGG++LEEDAA++L  LQ TLNG LDELA  FA S   R+  SV  +G LL  +KGGGQ    Q   ++ V + AD +L+PLMDLLDGSLS+YA+ C+K+VLKRLLKELWKIVM+ LEK +VLPP TDK+   K+L  + KN+A KTK +ED++  FK+   GKQDVK++ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK ++++KS EL+SL++ALSLYTQTTD LIKT+V++Q                                          + QD+   +EGSVGEVS+QVDLFTHPGTGEHKVTVKV+                     LIGPHLSDKKR   TKSK  NWSPKYNE F F IGNE+Q+  +ELHICVKDYCF R+DRL+GV+V+QLKDI ++GSCACWLSLG+RIQMDETGWTILRILSQRTNDEVAKEFVKLKS++R E+PL  Q
Sbjct:  535 DPNEPKRDPEDHGPRLDSVDFWHKLIALIVSVIEEDKNSYGPVLNQFPQELNIGQLSAATMWSLFAVDMKYALEEHEQHRLCKSSAYMNLHFKVKWLYSNYVKDVPPYKGAVPEYPAWFEPFVMQWLNENDDVSLEYLHGAFNRDKKDGFQRSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMKRFAKTIVKVLIAYADIVKGEFPEHVKEERIACILMNNIQQLRVQLEKMFESMGGEKLEEDAANILKDLQQTLNGCLDELATQFASSLEPRITVSVKELGDLLLSIKGGGQPGTTQPAQRNVVASEADEVLRPLMDLLDGSLSLYAQSCEKTVLKRLLKELWKIVMRILEKTVVLPPMTDKTMMFKSLTDNAKNLAAKTK-IEDMSTLFKNHMAGKQDVKNA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKAFLDKSPELQSLRYALSLYTQTTDTLIKTFVTTQINEVPLNNKETMLQRQISIERDETETGLEALEEPLKAKKRRESRQDTLDGQEGSVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWNVVSGMFRPFVEVNLIGPHLSDKKRKHATKSKANNWSPKYNETFHFTIGNEEQLDYFELHICVKDYCFAREDRLVGVAVMQLKDIVEKGSCACWLSLGKRIQMDETGWTILRILSQRTNDEVAKEFVKLKSDIRQENPLPNQ 1253          

HSP 2 Score: 560.066 bits (1442), Expect = 6.042e-180
Identity = 257/313 (82.11%), Postives = 287/313 (91.69%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+DKAN+II AMKERMK+RERE+PEIFELIR  FS+ PD HI+ + Q EQ  ++GTSKWSCKIA TV  AQGLIAKDK+GTSDPYVTVQVGK KKRT+T+PQELNP W+EKFYFECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G VSLP+A+G+DAWK+YF+E  +EIVDEFA+RYG+ESI+QAMTHFHCLS+KYLCPGVPA MS LLANINA+YAHTTA++ V
Sbjct:  114 LQRAAEKSSKHGAEDKANSIITAMKERMKQREREKPEIFELIRTTFSVDPDTHIDSIEQAEQMTIEGTSKWSCKIAITVKSAQGLIAKDKSGTSDPYVTVQVGKVKKRTRTMPQELNPVWDEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEQNSGVVSLPQAKGDDAWKVYFEEAGEEIVDEFAMRYGIESIYQAMTHFHCLSTKYLCPGVPAVMSMLLANINAYYAHTTASSAV 426          

BLAST of EMLSAG00000007474 vs. Select Arthropod Genomes
Match: gb|KXZ75743.1| (Protein unc-13 homolog B-like Protein [Tribolium castaneum])

HSP 1 Score: 960.288 bits (2481), Expect = 0.000e+0
Identity = 470/721 (65.19%), Postives = 555/721 (76.98%), Query Frame = 0

Query:  378 DPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ---GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQ------------------------------------------THQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQGQ 1031
            DPNE KRD ED GPRLD ++FWHKLIALI SVIEEDKNSYGPVLNQFPQELN+GQLSAAT+W LFAVD KYALEEHEQ RLCKSS Y+N HF+VKWL+  YV  VPPYKG VPEYPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQ+++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYMKRFAKTIVKVL AYA+I+KG+F  HVK+E  AC+LMNNIQQ R+QLEK + +MGG++LEEDAA++L  LQ TLNG LDELA  FA S   R+  SV  +G LL  +KGGGQ    Q   ++ V + AD +L+PLMDLLDGSLS+YA+ C+K+VLKRLLKELWKIVM+ LEK +VLPP TDK+   K+L  + KN+A KTK +ED++  FK+   GKQDVK++ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK ++++KS EL+SL++ALSLYTQTTD LIKT+V++Q                                          + QD+   +EGSVGEVS+QVDLFTHPGTGEHKVTVKV+                     LIGPHLSDKKR   TKSK  NWSPKYNE F F IGNE+Q+  +ELHICVKDYCF R+DRL+GV+V+QLKDI ++GSCACWLSLG+RIQMDETGWTILRILSQRTNDEVAKEFVKLKS++R E+PL  Q
Sbjct:  598 DPNEPKRDPEDHGPRLDSVDFWHKLIALIVSVIEEDKNSYGPVLNQFPQELNIGQLSAATMWSLFAVDMKYALEEHEQHRLCKSSAYMNLHFKVKWLYSNYVKDVPPYKGAVPEYPAWFEPFVMQWLNENDDVSLEYLHGAFNRDKKDGFQRSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMKRFAKTIVKVLIAYADIVKGEFPEHVKEERIACILMNNIQQLRVQLEKMFESMGGEKLEEDAANILKDLQQTLNGCLDELATQFASSLEPRITVSVKELGDLLLSIKGGGQPGTTQPAQRNVVASEADEVLRPLMDLLDGSLSLYAQSCEKTVLKRLLKELWKIVMRILEKTVVLPPMTDKTMMFKSLTDNAKNLAAKTK-IEDMSTLFKNHMAGKQDVKNA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKAFLDKSPELQSLRYALSLYTQTTDTLIKTFVTTQINEVPLNNKETMLQRQISIERDETETGLEALEEPLKAKKRRESRQDTLDGQEGSVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWNVVSGMFRPFVEVNLIGPHLSDKKRKHATKSKANNWSPKYNETFHFTIGNEEQLDYFELHICVKDYCFAREDRLVGVAVMQLKDIVEKGSCACWLSLGKRIQMDETGWTILRILSQRTNDEVAKEFVKLKSDIRQENPLPNQ 1316          

HSP 2 Score: 491.115 bits (1263), Expect = 3.181e-153
Identity = 223/275 (81.09%), Postives = 250/275 (90.91%), Query Frame = 0

Query:  107 RNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            R  FS+ PD HI+ + Q EQ  ++GTSKWSCKIA TV  AQGLIAKDK+GTSDPYVTVQVGK KKRT+T+PQELNP W+EKFYFECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G VSLP+A+G+DAWK+YF+E  +EIVDEFA+RYG+ESI+QAMTHFHCLS+KYLCPGVPA MS LLANINA+YAHTTA++ V  ++
Sbjct:  219 RTTFSVDPDTHIDSIEQAEQMTIEGTSKWSCKIAITVKSAQGLIAKDKSGTSDPYVTVQVGKVKKRTRTMPQELNPVWDEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEQNSGVVSLPQAKGDDAWKVYFEEAGEEIVDEFAMRYGIESIYQAMTHFHCLSTKYLCPGVPAVMSMLLANINAYYAHTTASSAVSASD 493          

BLAST of EMLSAG00000007474 vs. Select Arthropod Genomes
Match: gb|KXZ75742.1| (Protein unc-13 homolog B-like Protein [Tribolium castaneum])

HSP 1 Score: 960.288 bits (2481), Expect = 0.000e+0
Identity = 470/721 (65.19%), Postives = 555/721 (76.98%), Query Frame = 0

Query:  378 DPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ---GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQ------------------------------------------THQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQGQ 1031
            DPNE KRD ED GPRLD ++FWHKLIALI SVIEEDKNSYGPVLNQFPQELN+GQLSAAT+W LFAVD KYALEEHEQ RLCKSS Y+N HF+VKWL+  YV  VPPYKG VPEYPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQ+++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYMKRFAKTIVKVL AYA+I+KG+F  HVK+E  AC+LMNNIQQ R+QLEK + +MGG++LEEDAA++L  LQ TLNG LDELA  FA S   R+  SV  +G LL  +KGGGQ    Q   ++ V + AD +L+PLMDLLDGSLS+YA+ C+K+VLKRLLKELWKIVM+ LEK +VLPP TDK+   K+L  + KN+A KTK +ED++  FK+   GKQDVK++ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK ++++KS EL+SL++ALSLYTQTTD LIKT+V++Q                                          + QD+   +EGSVGEVS+QVDLFTHPGTGEHKVTVKV+                     LIGPHLSDKKR   TKSK  NWSPKYNE F F IGNE+Q+  +ELHICVKDYCF R+DRL+GV+V+QLKDI ++GSCACWLSLG+RIQMDETGWTILRILSQRTNDEVAKEFVKLKS++R E+PL  Q
Sbjct:  601 DPNEPKRDPEDHGPRLDSVDFWHKLIALIVSVIEEDKNSYGPVLNQFPQELNIGQLSAATMWSLFAVDMKYALEEHEQHRLCKSSAYMNLHFKVKWLYSNYVKDVPPYKGAVPEYPAWFEPFVMQWLNENDDVSLEYLHGAFNRDKKDGFQRSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMKRFAKTIVKVLIAYADIVKGEFPEHVKEERIACILMNNIQQLRVQLEKMFESMGGEKLEEDAANILKDLQQTLNGCLDELATQFASSLEPRITVSVKELGDLLLSIKGGGQPGTTQPAQRNVVASEADEVLRPLMDLLDGSLSLYAQSCEKTVLKRLLKELWKIVMRILEKTVVLPPMTDKTMMFKSLTDNAKNLAAKTK-IEDMSTLFKNHMAGKQDVKNA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKAFLDKSPELQSLRYALSLYTQTTDTLIKTFVTTQINEVPLNNKETMLQRQISIERDETETGLEALEEPLKAKKRRESRQDTLDGQEGSVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWNVVSGMFRPFVEVNLIGPHLSDKKRKHATKSKANNWSPKYNETFHFTIGNEEQLDYFELHICVKDYCFAREDRLVGVAVMQLKDIVEKGSCACWLSLGKRIQMDETGWTILRILSQRTNDEVAKEFVKLKSDIRQENPLPNQ 1319          

HSP 2 Score: 491.115 bits (1263), Expect = 3.174e-153
Identity = 223/275 (81.09%), Postives = 250/275 (90.91%), Query Frame = 0

Query:  107 RNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            R  FS+ PD HI+ + Q EQ  ++GTSKWSCKIA TV  AQGLIAKDK+GTSDPYVTVQVGK KKRT+T+PQELNP W+EKFYFECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G VSLP+A+G+DAWK+YF+E  +EIVDEFA+RYG+ESI+QAMTHFHCLS+KYLCPGVPA MS LLANINA+YAHTTA++ V  ++
Sbjct:  222 RTTFSVDPDTHIDSIEQAEQMTIEGTSKWSCKIAITVKSAQGLIAKDKSGTSDPYVTVQVGKVKKRTRTMPQELNPVWDEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEQNSGVVSLPQAKGDDAWKVYFEEAGEEIVDEFAMRYGIESIYQAMTHFHCLSTKYLCPGVPAVMSMLLANINAYYAHTTASSAVSASD 496          

BLAST of EMLSAG00000007474 vs. Select Arthropod Genomes
Match: EFX71298.1 (hypothetical protein DAPPUDRAFT_60333 [Daphnia pulex])

HSP 1 Score: 927.546 bits (2396), Expect = 0.000e+0
Identity = 458/709 (64.60%), Postives = 542/709 (76.45%), Query Frame = 0

Query:  343 CLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ---GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFK-SFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGED 1026
            CL S Y           L  N    YA        +P EAKRD ED GPRLD ++FWHKLIALI SVIEEDKNSY PVLNQFPQELN+GQLSA T+W  FAVD KYALEEHEQ RLCKSS Y+N HF+VKWL+  YV  +P YKG VPEYPAWFEPFVMQWLNENDDVSLE+LH A+ RDK+D FQ+++EH+ FS SVVD+FTQL QCF+V+ KLEC DPEIWKRYMKRFAKTIVKVL AYA+  K +F    KDE  AC LMNNIQQ R+QLEK +  MGG +LEEDAA++L +LQ +LN VLDELA +FA S   ++  SV  +G LL  +KGGGQ    Q   ++ V   A+  L+PLMDLLDGSLS+YAE C+K+VLKRLLKELWKIVM ++EK IVLPP TDKS  +KNL  + KN+A    K+ED++  FK S  GKQDVKS+ +S V +ISKE E++L+PKQC+VL+ AL+ IKQYFHAGG GLK +++ KS EL+SL++ALSLYTQTTD LIKT+V++QT+QD  S EEGSVGEVSVQVDLFTHPG+GEHKVTVKV+                     L+GPHL+DKKR   TKSK+  WSPKYNE F F IGNE+ + S+ELHICVKDYCF RDD L+GV+V+QLKDI +QGSCACWL+LG+R  MDETGWTILRILSQRTNDEVA+EFVK+KSEVR E+
Sbjct:  598 CLKSTY---------QFLFENCYELYAREY---QAEPGEAKRDPEDHGPRLDSVDFWHKLIALIVSVIEEDKNSYAPVLNQFPQELNIGQLSAYTMWTQFAVDMKYALEEHEQQRLCKSSAYMNLHFKVKWLYNHYVKDLPQYKGAVPEYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKQDGFQRSSEHTLFSTSVVDIFTQLAQCFDVVSKLECPDPEIWKRYMKRFAKTIVKVLLAYADTAKKEFPQLTKDERIACTLMNNIQQLRVQLEKLFEQMGGSKLEEDAANILKELQQSLNNVLDELATMFAKSLEPKITVSVKEVGDLLSHIKGGGQVSLTQPSQRNAVSTEAEDSLKPLMDLLDGSLSLYAESCEKAVLKRLLKELWKIVMHTMEKTIVLPPMTDKSMILKNLTDNAKNLAANA-KIEDMSRLFKNSVAGKQDVKSA-LSGVMEISKEFEKNLTPKQCAVLDVALDTIKQYFHAGGSGLKKTFLEKSLELQSLRYALSLYTQTTDTLIKTFVTTQTNQDLPSVEEGSVGEVSVQVDLFTHPGSGEHKVTVKVVAANDLKWLVQQGIFRPFVETNLVGPHLADKKRKFATKSKSNTWSPKYNETFHFAIGNEETLESFELHICVKDYCFARDDSLVGVAVMQLKDIVEQGSCACWLALGKRCHMDETGWTILRILSQRTNDEVAREFVKMKSEVRPEE 1292          

HSP 2 Score: 532.332 bits (1370), Expect = 5.428e-169
Identity = 249/318 (78.30%), Postives = 281/318 (88.36%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEK-NGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            +  AAEKSSKHGA+DK   II AMKERMK+RER +PEIFELIR +F +   +H   M   +Q+VLDGTSKWS KIA TVICAQGLIAKDK+GTSDPYVTVQVGK KKRT+T+PQELNP WNE+FYFECHNSSDRIKVRVWDEDND KSK+RQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYL E+ + G V LP A+G+D+WKIYFDE  QE+V+EFA+R+G+E I+QAMT+FHCLS+KYLCPGVPA MSTLLANINAFYAHTTA+T V  ++
Sbjct:  199 LQRAAEKSSKHGAEDKTQIIITAMKERMKQRERTKPEIFELIRAVFGVEERSHNGHMKAVKQSVLDGTSKWSAKIAITVICAQGLIAKDKSGTSDPYVTVQVGKVKKRTRTMPQELNPIWNERFYFECHNSSDRIKVRVWDEDNDFKSKMRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLSEQVHHGGVKLPVAKGDDSWKIYFDEPSQELVEEFAMRFGIEPIYQAMTNFHCLSTKYLCPGVPAVMSTLLANINAFYAHTTASTAVSASD 516          

BLAST of EMLSAG00000007474 vs. Select Arthropod Genomes
Match: AGB96565.1 (unc-13, isoform F [Drosophila melanogaster])

HSP 1 Score: 926.006 bits (2392), Expect = 0.000e+0
Identity = 447/681 (65.64%), Postives = 534/681 (78.41%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGG-----GQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQG 1030
            VDPNEAKR  +D  P+LD ++FWHKLIALI SVI+EDKNSYG VLNQFPQELN+GQLSA+++W LFAVD KYALEEHEQ RLCKSS Y+N HFRVKWL+  YV  VPPYKG VP+YPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQK++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYM+RFAKTIVKVL AYA+I+K +F  H+KDE  AC+LMNNIQQ R+QLEK + +MGG +LEEDAA++L +LQ  LN  LD+LA  FA+S   R+  SV  +G +L  +KGG         A  ++ V   AD +L+PLMDLLDGSL++YA+ C+K+VLKRLLKELWKIVM+ LEK IVLPP TDK+   K+L  + KN+A    K+ED+   FKS   GKQDVKS+ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK +++ KS EL+SL++ALSLYTQ TD LIKT++SSQ H+    + E SVGE+SVQ+DLF+HPGTGEHKV VKV+                     LIGPHL +KKR   TKSK+ NWSPKYNE FSF IGNE+Q+  +ELHICVKDYCF RDDRL+GV+V+ LKDI ++GS ACWL L RRI+MDETGWTILRILSQR NDEVAKEFVKLKSE+R E P  G
Sbjct: 2033 VDPNEAKRAPDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFPQELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVPDYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEHMKDERIACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLPPMTDKTMMFKHLTDNAKNLASNA-KIEDMGRLFKSHMAGKQDVKSA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFISSQVHEVDLENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIPSGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCFARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEIRQE-PTMG 2710          

HSP 2 Score: 391.349 bits (1004), Expect = 6.558e-114
Identity = 220/430 (51.16%), Postives = 249/430 (57.91%), Query Frame = 0

Query:   13 GNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL-----------------------------------------------------KLHVYKKXLQALIYPISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIA---------KDKTGTSD---PYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            G R+N    DNPF+ +IDSMP + RPRRKSIPLVSEL                                                     K+HVYKK LQALIYPISS                                P  F L     + SP    E           G     C + C   C   L A           K G  D     +T    + K+R +  P+        +   ECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G V LP  +G+DAWK+YFDE  +EIVDEF++RYG+E+I+QAMTHFHCLS+KYLCPGVPA MSTLLANINA+YAHTTA++ V
Sbjct: 1536 GLRTNGHPGDNPFYSNIDSMP-DIRPRRKSIPLVSELVLKANYPNAPSKMDQCYLLSLQENVETTTMAATKRNAGLTSAVPRATLNDEDLKMHVYKKALQALIYPISSTT------------------------------PHNFLLWT---ATSPTYCYECEGLLWGIARQGVRCTECGVKCHEKCKDLLNADCLQRAAEKSSKHGAEDKANSIITAMKDRMKQREREKPEIF------ELISECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHFHCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAV 1925          

BLAST of EMLSAG00000007474 vs. Select Arthropod Genomes
Match: AFH06787.1 (unc-13, isoform E [Drosophila melanogaster])

HSP 1 Score: 925.235 bits (2390), Expect = 0.000e+0
Identity = 447/681 (65.64%), Postives = 534/681 (78.41%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGG-----GQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQG 1030
            VDPNEAKR  +D  P+LD ++FWHKLIALI SVI+EDKNSYG VLNQFPQELN+GQLSA+++W LFAVD KYALEEHEQ RLCKSS Y+N HFRVKWL+  YV  VPPYKG VP+YPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQK++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYM+RFAKTIVKVL AYA+I+K +F  H+KDE  AC+LMNNIQQ R+QLEK + +MGG +LEEDAA++L +LQ  LN  LD+LA  FA+S   R+  SV  +G +L  +KGG         A  ++ V   AD +L+PLMDLLDGSL++YA+ C+K+VLKRLLKELWKIVM+ LEK IVLPP TDK+   K+L  + KN+A    K+ED+   FKS   GKQDVKS+ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK +++ KS EL+SL++ALSLYTQ TD LIKT++SSQ H+    + E SVGE+SVQ+DLF+HPGTGEHKV VKV+                     LIGPHL +KKR   TKSK+ NWSPKYNE FSF IGNE+Q+  +ELHICVKDYCF RDDRL+GV+V+ LKDI ++GS ACWL L RRI+MDETGWTILRILSQR NDEVAKEFVKLKSE+R E P  G
Sbjct: 2508 VDPNEAKRAPDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFPQELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVPDYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEHMKDERIACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLPPMTDKTMMFKHLTDNAKNLASNA-KIEDMGRLFKSHMAGKQDVKSA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFISSQVHEVDLENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIPSGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCFARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEIRQE-PTMG 3185          

HSP 2 Score: 550.436 bits (1417), Expect = 4.627e-168
Identity = 253/313 (80.83%), Postives = 284/313 (90.73%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+DKAN+II AMK+RMK+RERE+PEIFELIR +FS+   +H   M   +Q+VLDGTSKWS KIA TVICAQGLIAKDK+GTSDPYVTVQV K KKRT+T+PQELNP WNEKF+FECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G V LP  +G+DAWK+YFDE  +EIVDEF++RYG+E+I+QAMTHFHCLS+KYLCPGVPA MSTLLANINA+YAHTTA++ V
Sbjct: 2088 LQRAAEKSSKHGAEDKANSIITAMKDRMKQREREKPEIFELIRAVFSVEEKSHAGHMKAVKQSVLDGTSKWSAKIAITVICAQGLIAKDKSGTSDPYVTVQVSKVKKRTRTMPQELNPVWNEKFHFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHFHCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAV 2400          

BLAST of EMLSAG00000007474 vs. Select Arthropod Genomes
Match: AAN06593.1 (unc-13, isoform B [Drosophila melanogaster])

HSP 1 Score: 925.235 bits (2390), Expect = 0.000e+0
Identity = 447/681 (65.64%), Postives = 534/681 (78.41%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGG-----GQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQG 1030
            VDPNEAKR  +D  P+LD ++FWHKLIALI SVI+EDKNSYG VLNQFPQELN+GQLSA+++W LFAVD KYALEEHEQ RLCKSS Y+N HFRVKWL+  YV  VPPYKG VP+YPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQK++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYM+RFAKTIVKVL AYA+I+K +F  H+KDE  AC+LMNNIQQ R+QLEK + +MGG +LEEDAA++L +LQ  LN  LD+LA  FA+S   R+  SV  +G +L  +KGG         A  ++ V   AD +L+PLMDLLDGSL++YA+ C+K+VLKRLLKELWKIVM+ LEK IVLPP TDK+   K+L  + KN+A    K+ED+   FKS   GKQDVKS+ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK +++ KS EL+SL++ALSLYTQ TD LIKT++SSQ H+    + E SVGE+SVQ+DLF+HPGTGEHKV VKV+                     LIGPHL +KKR   TKSK+ NWSPKYNE FSF IGNE+Q+  +ELHICVKDYCF RDDRL+GV+V+ LKDI ++GS ACWL L RRI+MDETGWTILRILSQR NDEVAKEFVKLKSE+R E P  G
Sbjct: 2505 VDPNEAKRAPDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFPQELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVPDYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEHMKDERIACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLPPMTDKTMMFKHLTDNAKNLASNA-KIEDMGRLFKSHMAGKQDVKSA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFISSQVHEVDLENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIPSGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCFARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEIRQE-PTMG 3182          

HSP 2 Score: 550.436 bits (1417), Expect = 4.668e-168
Identity = 253/313 (80.83%), Postives = 284/313 (90.73%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+DKAN+II AMK+RMK+RERE+PEIFELIR +FS+   +H   M   +Q+VLDGTSKWS KIA TVICAQGLIAKDK+GTSDPYVTVQV K KKRT+T+PQELNP WNEKF+FECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G V LP  +G+DAWK+YFDE  +EIVDEF++RYG+E+I+QAMTHFHCLS+KYLCPGVPA MSTLLANINA+YAHTTA++ V
Sbjct: 2085 LQRAAEKSSKHGAEDKANSIITAMKDRMKQREREKPEIFELIRAVFSVEEKSHAGHMKAVKQSVLDGTSKWSAKIAITVICAQGLIAKDKSGTSDPYVTVQVSKVKKRTRTMPQELNPVWNEKFHFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHFHCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAV 2397          

HSP 3 Score: 73.1738 bits (178), Expect = 5.794e-12
Identity = 41/82 (50.00%), Postives = 46/82 (56.10%), Query Frame = 0

Query:   13 GNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL-------------------------KLHVYKKXLQALIYPISSAA 69
            G R+N    DNPF+ +IDSMP + RPRRKSIPLVSEL                         K+HVYKK LQALIYPISS  
Sbjct: 1953 GLRTNGHPGDNPFYSNIDSMP-DIRPRRKSIPLVSELTMAATKRNAGLTSAVPRATLNDEDLKMHVYKKALQALIYPISSTT 2033          

BLAST of EMLSAG00000007474 vs. Select Arthropod Genomes
Match: AHN58218.1 (unc-13, isoform G [Drosophila melanogaster])

HSP 1 Score: 924.465 bits (2388), Expect = 0.000e+0
Identity = 447/681 (65.64%), Postives = 534/681 (78.41%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGG-----GQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQG 1030
            VDPNEAKR  +D  P+LD ++FWHKLIALI SVI+EDKNSYG VLNQFPQELN+GQLSA+++W LFAVD KYALEEHEQ RLCKSS Y+N HFRVKWL+  YV  VPPYKG VP+YPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQK++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYM+RFAKTIVKVL AYA+I+K +F  H+KDE  AC+LMNNIQQ R+QLEK + +MGG +LEEDAA++L +LQ  LN  LD+LA  FA+S   R+  SV  +G +L  +KGG         A  ++ V   AD +L+PLMDLLDGSL++YA+ C+K+VLKRLLKELWKIVM+ LEK IVLPP TDK+   K+L  + KN+A    K+ED+   FKS   GKQDVKS+ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK +++ KS EL+SL++ALSLYTQ TD LIKT++SSQ H+    + E SVGE+SVQ+DLF+HPGTGEHKV VKV+                     LIGPHL +KKR   TKSK+ NWSPKYNE FSF IGNE+Q+  +ELHICVKDYCF RDDRL+GV+V+ LKDI ++GS ACWL L RRI+MDETGWTILRILSQR NDEVAKEFVKLKSE+R E P  G
Sbjct: 2193 VDPNEAKRAPDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFPQELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVPDYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEHMKDERIACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLPPMTDKTMMFKHLTDNAKNLASNA-KIEDMGRLFKSHMAGKQDVKSA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFISSQVHEVDLENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIPSGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCFARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEIRQE-PTMG 2870          

HSP 2 Score: 550.051 bits (1416), Expect = 5.330e-168
Identity = 253/310 (81.61%), Postives = 283/310 (91.29%), Query Frame = 0

Query:   68 AAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            AAEKSSKHGA+DKAN+II AMK+RMK+RERE+PEIFELIR +FS+   +H   M   +Q+VLDGTSKWS KIA TVICAQGLIAKDK+GTSDPYVTVQV K KKRT+T+PQELNP WNEKF+FECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G V LP  +G+DAWK+YFDE  +EIVDEF++RYG+E+I+QAMTHFHCLS+KYLCPGVPA MSTLLANINA+YAHTTA++ V
Sbjct: 1776 AAEKSSKHGAEDKANSIITAMKDRMKQREREKPEIFELIRAVFSVEEKSHAGHMKAVKQSVLDGTSKWSAKIAITVICAQGLIAKDKSGTSDPYVTVQVSKVKKRTRTMPQELNPVWNEKFHFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHFHCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAV 2085          

HSP 3 Score: 72.7886 bits (177), Expect = 6.339e-12
Identity = 41/82 (50.00%), Postives = 46/82 (56.10%), Query Frame = 0

Query:   13 GNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL-------------------------KLHVYKKXLQALIYPISSAA 69
            G R+N    DNPF+ +IDSMP + RPRRKSIPLVSEL                         K+HVYKK LQALIYPISS  
Sbjct: 1641 GLRTNGHPGDNPFYSNIDSMP-DIRPRRKSIPLVSELTMAATKRNAGLTSAVPRATLNDEDLKMHVYKKALQALIYPISSTT 1721          

BLAST of EMLSAG00000007474 vs. nr
Match: gi|982311345|ref|XP_015295697.1| (PREDICTED: protein unc-13 homolog A [Macaca fascicularis])

HSP 1 Score: 1213.75 bits (3139), Expect = 0.000e+0
Identity = 599/1044 (57.38%), Postives = 759/1044 (72.70%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCE-KNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVD--------------------------------------------------PNEAKR---DSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHV--KDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNL---------AGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            +  AAEKSSKHGA+D+   II+ +K+RMK RER +PEIFELI+ IF+++   H + M   +Q+VLDGTSKWS KI+ TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W E F+FECHNSSDRIKVRVWDED+D+KS+++Q+F RESDDFLGQTIIEVRTLSGEMDVWYNL+KR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFH++ + +N G V +P+A+G+DAWK+Y+DE  QEIVDEFA+RYGVESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV                                                   P +AK+     E+ GP + +L+FW KLI LI S+IEEDKNSY P LNQFPQELNVG++SA  +W LFA D KYA+EEH++ RLCKS+ Y+N HF+VKWL+  YV+ +P +K +VPEYPAWFEPFV+QWL+EN++VS +FLH A  RDKKD FQ+ +EH+ FS SVVDVF+QL Q FE+++KLEC DP+I   YM+RFAKTI  VL  YA+I+  DF ++   + E   C+LMNN QQ R+QLEK + AMGG+EL+ +A+ +L +LQ  LN VLDEL+ +FA SF   ++  V  MG +L +VKG G   A + S V   AD +LQP+MDLLD +L+++A+IC+K+VLKR+LKELWK+VM ++EK IVLPP TD++  I NL          G VK +   +   + +    K  G    +K  ++       +E  +SL+PKQC+V+E AL+ IKQYFHAGG+GLK +++ KS +L+SL++ALSLYTQ TD LIKT+V +Q+ Q      E  VGEVSV V+LFTHPGTGEHKVTVKV+                    +IGP LSDKKR   TKSKN +W+PKYNE F F +  +     YEL +CVKDYCF R+DR +G++V+QL+++  +GS ACWL LGRRI MD+TG T+LRILSQR+NDEVAKEFVKLKS+ R
Sbjct:  624 LQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKTAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGVSPGDWDWRMRESPTLCRWVELPCVYTNLWTPYNRGLHCPFQAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWNLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVTELPAFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIISKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASDILKELQVKLNNVLDELSRVFATSFQPHIEECVKQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQT-MIGNLLRKHGKGLEKGRVK-LPSHSDGTQMIFNAAKELGQLSKLKDHMV-------REEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQ--GLGVEDPVGEVSVHVELFTHPGTGEHKVTVKVVAANDLKWQTSGIFRPFIEVNIIGPQLSDKKRKFATKSKNNSWAPKYNESFQFTLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTR 1656          

BLAST of EMLSAG00000007474 vs. nr
Match: gi|850271891|ref|XP_012863161.1| (PREDICTED: protein unc-13 homolog A [Echinops telfairi])

HSP 1 Score: 1140.95 bits (2950), Expect = 0.000e+0
Identity = 568/1017 (55.85%), Postives = 735/1017 (72.27%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCE-KNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQ----------------------AMTHFHCLSSKYLCPGVPAQM--STLLANINAFY------AHTTATTNVDPNEAKR---DSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHV--KDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAE-KTKKVEDVTGFFKSFGGKQDVKSSVMSVVSD-ISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            +  AAEKSSKHGA+D+   II+ +K+RMK RER +PEIFELI+ IF+++   H + M   +Q+VLDGTSKWS KI+ TV+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W E F+FECHNSSDRIKVRVWDED+D+KS+++Q+F RESDDFLGQTIIEVRTLSGEMDVWYNL+KR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFH++ + +N G V +P+A+G+DAWK+Y+DE  QEIVDEFA+RYGVESI+Q                      ++T F     +   P   +Q+    + A +N+ Y       H   +     + AK+     E+ GP + +L+FW KLI LI S+IEEDKNSY P LNQFPQELNVG++SA  +W LFA D KYA+EEH++ RLCKS+ Y+N HF+VKWL+  YV+ +P +K +VPEYPAWFEPFV+QWL+EN++VS +FLH A  RDKKD FQ+ +EH+ FS SVVDVF+QL Q FE+++KLEC DP+I   YM+RFAKTI  VL  YA+I+  DF  +   + E   C+LMNN QQ R+QLEK + AMGG+EL+ +A+ +L +LQ  LN VLDEL+ +FA SF   ++  V  MG +L +VKG G   A + S V   AD +LQP+MDLLD +L+++A+IC+K+VLKR+LKELWK+VM ++EK IVLPP TD++     L    K + + + K      G    F   +++    +S + D + +E  +SL+PKQC+V+E AL+ IKQYFHAGG+GLK +++ KS +L+SL++ALSLYTQ TD LIKT+V +Q  Q S   +   VGEVSV V+LFTHPGTGEHKVTVKV+                    +IGP LSDKKR   TKSKN +W+PKYNE F F +  +     YEL +CVKDYCF R+DR +G++V+QL+++  +GS ACWL LGRRI MD+TG T+LRILSQR+NDEVAKEFVKLKS+ R
Sbjct:  638 LQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEIFAVTKTAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYSREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWNLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVTELPSFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIISKDFACYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASDILKELQVKLNNVLDELSRVFATSFQPHIEECVKQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQ--LSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQAAQGSGVDD--PVGEVSVHVELFTHPGTGEHKVTVKVVAANDLKWQTSGIFRPFIEVNIIGPQLSDKKRKFATKSKNNSWAPKYNESFQFTLSADTGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTR 1650          

BLAST of EMLSAG00000007474 vs. nr
Match: gi|795076676|ref|XP_011846576.1| (PREDICTED: protein unc-13 homolog A [Mandrillus leucophaeus])

HSP 1 Score: 1139.79 bits (2947), Expect = 0.000e+0
Identity = 555/930 (59.68%), Postives = 702/930 (75.48%), Query Frame = 0

Query:  137 CKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCE-KNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE--------AKR---DSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHV--KDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNL---------AGDVKNMAEKTKKVEDVTGFFKSFGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVR 1023
            C +   V+CAQGL AKDKTG+SDPYVTVQVGK+KKRTKT+   LNP W E F+FECHNSSDRIKVRVWDED+D+KS+++Q+F RESDDFLGQTIIEVRTLSGEMDVWYNL+KR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFH++ + +N G V +P+A+G+DAWK+Y+DE  QEIVDEFA+RYGVESI+QAMTHF CLSSKY+CPGVPA MSTLLANINA+YAHTTA+TNV  ++        AK+     E+ GP + +L+FW KLI LI S+IEEDKNSY P LNQFPQELNVG++SA  +W LFA D KYA+EEH++ RLCKS+ Y+N HF+VKWL+  YV+ +P +K +VPEYPAWFEPFV+QWL+EN++VS +FLH A  RDKKD FQ+ +EH+ FS SVVDVF+QL Q FE+++KLEC DP+I   YM+RFAKTI  VL  YA+I+  DF ++   + E   C+LMNN QQ R+QLEK + AMGG+EL+ +A+ +L +LQ  LN VLDEL+ +FA SF   ++  V  MG +L +VKG G   A + S V   AD +LQP+MDLLD +L+++A+IC+K+VLKR+LKELWK+VM ++EK IVLPP TD++  I NL          G VK +   +   + +    K  G    +K  ++       +E  +SL+PKQC+V+E AL+ IKQYFHAGG+GLK +++ KS +L+SL++ALSLYTQ TD LIKT+V +Q+ Q      E  VGEVSV V+LFTHPGTGEHKVTVKV+                    +IGP LSDKKR   TKSKN +W+PKYNE F F +  +     YEL +CVKDYCF R+DR +G++V+QL+++  +GS ACWL LGRRI MD+TG T+LRILSQR+NDEVAKEFVKLKS+ R
Sbjct:  654 CPLCLPVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWNLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVTELPAFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIISKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASDILKELQVKLNNVLDELSRVFATSFQPHIEECVKQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPPLTDQT-MIGNLLRKHGKGLEKGRVK-LPSHSDGTQMIFNAAKELGQLSKLKDHMV-------REEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQ--GLGVEDPVGEVSVHVELFTHPGTGEHKVTVKVVAANDLKWQTSGIFRPFIEVNIIGPQLSDKKRKFATKSKNNSWAPKYNESFQFTLSADAGPECYELQVCVKDYCFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTR 1572          

BLAST of EMLSAG00000007474 vs. nr
Match: gi|1069817546|ref|XP_018331998.1| (PREDICTED: protein unc-13 homolog A isoform X5 [Agrilus planipennis])

HSP 1 Score: 982.63 bits (2539), Expect = 0.000e+0
Identity = 467/676 (69.08%), Postives = 553/676 (81.80%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ----GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGED 1026
             DPNE KRD ED GPRLD ++FWHKLIALI SVIEEDKNSYGPVLNQFPQELN+GQLSAAT+W LFAVD KYALEEHEQ RLCKSS Y+N HF+VKWL+  YV  VPPYKG VPEYP+WFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQK++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYMKRFAKTIVKVL AYA+I+K +F  H+K+E  AC+LMNNIQQ R+QLEK + +MGG++LEEDAA++L +LQ +LNG LDELA LFA S   R+  SV  +G LL  +KGGGQ     Q   ++ V   AD +L+PLMDLLDGSLS+YA+ C+K+VLKRLLKELWKIVM+ LEK +VLPP TDK+   K+L  + KN+A  T K+ED++  FK+   GKQDVK++ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK ++++KS EL+SL++ALSLYTQTTD LIKT+V+SQ ++D++ S+EG VGEVS+QVDLFTHPGTGEHKVTVKV+                     LIGPHLSDKKR   TKSK  NWSPKYNE F FIIGNE+Q+  YELHICVKDYCF R+DRL+GV+V+QLKDI D+GSCACWLSLG+RIQMDETGWTILRILSQRTNDEVAKEFVKLKS++R E+
Sbjct: 2057 ADPNEPKRDPEDHGPRLDSVDFWHKLIALIVSVIEEDKNSYGPVLNQFPQELNIGQLSAATMWSLFAVDMKYALEEHEQHRLCKSSAYMNLHFKVKWLYTNYVKDVPPYKGAVPEYPSWFEPFVMQWLNENDDVSLEYLHGAFNRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMKRFAKTIVKVLIAYADIVKREFPEHLKEERIACILMNNIQQLRVQLEKMFESMGGEKLEEDAANILKELQQSLNGALDELASLFASSLEPRITQSVKELGDLLLAIKGGGQPGTLNQPAQRNAVAVEADEVLRPLMDLLDGSLSLYAQSCEKTVLKRLLKELWKIVMRILEKTVVLPPMTDKTMVFKSLTDNAKNLAANT-KIEDMSRIFKNHMTGKQDVKNA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKAFLDKSSELQSLRYALSLYTQTTDTLIKTFVTSQVNEDTTDSQEGCVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWTVVSGMFRPFVEVNLIGPHLSDKKRKHATKSKANNWSPKYNETFHFIIGNEEQLDFYELHICVKDYCFAREDRLVGVAVMQLKDIVDRGSCACWLSLGKRIQMDETGWTILRILSQRTNDEVAKEFVKLKSDIRQEN 2730          

HSP 2 Score: 568.54 bits (1464), Expect = 6.442e-172
Identity = 257/313 (82.11%), Postives = 289/313 (92.33%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+DKAN+II AMKERMK+RERE+PEIFELIR  F++ PD HI+ + Q E+  ++GTSKWSCKIA TV CAQGLIAKDK+GTSDPYVTVQVGK KKRT+T+PQELNP W+EKFYFECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G V+LP+A+G+DAWK+YFDE  +EIVDEFA+RYG+ESI+QAMTHFHCLS+KYLCPGVPA MSTLLANINA+YAHTTA++ V
Sbjct: 1637 LQRAAEKSSKHGAEDKANSIITAMKERMKQREREKPEIFELIRTTFNVDPDTHIDSIEQAEEMTIEGTSKWSCKIAITVKCAQGLIAKDKSGTSDPYVTVQVGKVKKRTRTMPQELNPVWDEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEQNSGVVNLPQAKGDDAWKVYFDEAPEEIVDEFAMRYGIESIYQAMTHFHCLSTKYLCPGVPAVMSTLLANINAYYAHTTASSAV 1949          

BLAST of EMLSAG00000007474 vs. nr
Match: gi|1008456644|ref|XP_015840294.1| (PREDICTED: protein unc-13 homolog B isoform X6 [Tribolium castaneum])

HSP 1 Score: 982.63 bits (2539), Expect = 0.000e+0
Identity = 469/679 (69.07%), Postives = 555/679 (81.74%), Query Frame = 0

Query:  378 DPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ---GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQGQ 1031
            DPNE KRD ED GPRLD ++FWHKLIALI SVIEEDKNSYGPVLNQFPQELN+GQLSAAT+W LFAVD KYALEEHEQ RLCKSS Y+N HF+VKWL+  YV  VPPYKG VPEYPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQ+++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYMKRFAKTIVKVL AYA+I+KG+F  HVK+E  AC+LMNNIQQ R+QLEK + +MGG++LEEDAA++L  LQ TLNG LDELA  FA S   R+  SV  +G LL  +KGGGQ    Q   ++ V + AD +L+PLMDLLDGSLS+YA+ C+K+VLKRLLKELWKIVM+ LEK +VLPP TDK+   K+L  + KN+A KT K+ED++  FK+   GKQDVK++ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK ++++KS EL+SL++ALSLYTQTTD LIKT+V++Q ++D+   +EGSVGEVS+QVDLFTHPGTGEHKVTVKV+                     LIGPHLSDKKR   TKSK  NWSPKYNE F F IGNE+Q+  +ELHICVKDYCF R+DRL+GV+V+QLKDI ++GSCACWLSLG+RIQMDETGWTILRILSQRTNDEVAKEFVKLKS++R E+PL  Q
Sbjct: 1857 DPNEPKRDPEDHGPRLDSVDFWHKLIALIVSVIEEDKNSYGPVLNQFPQELNIGQLSAATMWSLFAVDMKYALEEHEQHRLCKSSAYMNLHFKVKWLYSNYVKDVPPYKGAVPEYPAWFEPFVMQWLNENDDVSLEYLHGAFNRDKKDGFQRSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMKRFAKTIVKVLIAYADIVKGEFPEHVKEERIACILMNNIQQLRVQLEKMFESMGGEKLEEDAANILKDLQQTLNGCLDELATQFASSLEPRITVSVKELGDLLLSIKGGGQPGTTQPAQRNVVASEADEVLRPLMDLLDGSLSLYAQSCEKTVLKRLLKELWKIVMRILEKTVVLPPMTDKTMMFKSLTDNAKNLAAKT-KIEDMSTLFKNHMAGKQDVKNA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKAFLDKSPELQSLRYALSLYTQTTDTLIKTFVTTQINEDTLDGQEGSVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWNVVSGMFRPFVEVNLIGPHLSDKKRKHATKSKANNWSPKYNETFHFTIGNEEQLDYFELHICVKDYCFAREDRLVGVAVMQLKDIVEKGSCACWLSLGKRIQMDETGWTILRILSQRTNDEVAKEFVKLKSDIRQENPLPNQ 2533          

HSP 2 Score: 548.895 bits (1413), Expect = 2.118e-165
Identity = 254/310 (81.94%), Postives = 283/310 (91.29%), Query Frame = 0

Query:   68 AAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            AAEKSSKHGA+DKAN+II AMKERMK+RERE+PEIFELIR +F +   +H   M   +Q+VLDGTSKWS KIA TV  AQGLIAKDK+GTSDPYVTVQVGK KKRT+T+PQELNP W+EKFYFECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G VSLP+A+G+DAWK+YF+E  +EIVDEFA+RYG+ESI+QAMTHFHCLS+KYLCPGVPA MS LLANINA+YAHTTA++ V
Sbjct: 1439 AAEKSSKHGAEDKANSIITAMKERMKQREREKPEIFELIRAVFGVEDKSHAGHMKAVKQSVLDGTSKWSAKIAITVKSAQGLIAKDKSGTSDPYVTVQVGKVKKRTRTMPQELNPVWDEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEQNSGVVSLPQAKGDDAWKVYFEEAGEEIVDEFAMRYGIESIYQAMTHFHCLSTKYLCPGVPAVMSMLLANINAYYAHTTASSAV 1748          

BLAST of EMLSAG00000007474 vs. nr
Match: gi|91093435|ref|XP_969667.1| (PREDICTED: protein unc-13 homolog B isoform X7 [Tribolium castaneum])

HSP 1 Score: 982.63 bits (2539), Expect = 0.000e+0
Identity = 469/679 (69.07%), Postives = 555/679 (81.74%), Query Frame = 0

Query:  378 DPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ---GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQGQ 1031
            DPNE KRD ED GPRLD ++FWHKLIALI SVIEEDKNSYGPVLNQFPQELN+GQLSAAT+W LFAVD KYALEEHEQ RLCKSS Y+N HF+VKWL+  YV  VPPYKG VPEYPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQ+++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYMKRFAKTIVKVL AYA+I+KG+F  HVK+E  AC+LMNNIQQ R+QLEK + +MGG++LEEDAA++L  LQ TLNG LDELA  FA S   R+  SV  +G LL  +KGGGQ    Q   ++ V + AD +L+PLMDLLDGSLS+YA+ C+K+VLKRLLKELWKIVM+ LEK +VLPP TDK+   K+L  + KN+A KT K+ED++  FK+   GKQDVK++ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK ++++KS EL+SL++ALSLYTQTTD LIKT+V++Q ++D+   +EGSVGEVS+QVDLFTHPGTGEHKVTVKV+                     LIGPHLSDKKR   TKSK  NWSPKYNE F F IGNE+Q+  +ELHICVKDYCF R+DRL+GV+V+QLKDI ++GSCACWLSLG+RIQMDETGWTILRILSQRTNDEVAKEFVKLKS++R E+PL  Q
Sbjct: 1836 DPNEPKRDPEDHGPRLDSVDFWHKLIALIVSVIEEDKNSYGPVLNQFPQELNIGQLSAATMWSLFAVDMKYALEEHEQHRLCKSSAYMNLHFKVKWLYSNYVKDVPPYKGAVPEYPAWFEPFVMQWLNENDDVSLEYLHGAFNRDKKDGFQRSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMKRFAKTIVKVLIAYADIVKGEFPEHVKEERIACILMNNIQQLRVQLEKMFESMGGEKLEEDAANILKDLQQTLNGCLDELATQFASSLEPRITVSVKELGDLLLSIKGGGQPGTTQPAQRNVVASEADEVLRPLMDLLDGSLSLYAQSCEKTVLKRLLKELWKIVMRILEKTVVLPPMTDKTMMFKSLTDNAKNLAAKT-KIEDMSTLFKNHMAGKQDVKNA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKAFLDKSPELQSLRYALSLYTQTTDTLIKTFVTTQINEDTLDGQEGSVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWNVVSGMFRPFVEVNLIGPHLSDKKRKHATKSKANNWSPKYNETFHFTIGNEEQLDYFELHICVKDYCFAREDRLVGVAVMQLKDIVEKGSCACWLSLGKRIQMDETGWTILRILSQRTNDEVAKEFVKLKSDIRQENPLPNQ 2512          

HSP 2 Score: 548.895 bits (1413), Expect = 1.831e-165
Identity = 254/313 (81.15%), Postives = 284/313 (90.73%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+DKAN+II AMKERMK+RERE+PEIFELIR +F +   +H   M   +Q+VLDGTSKWS KIA TV  AQGLIAKDK+GTSDPYVTVQVGK KKRT+T+PQELNP W+EKFYFECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G VSLP+A+G+DAWK+YF+E  +EIVDEFA+RYG+ESI+QAMTHFHCLS+KYLCPGVPA MS LLANINA+YAHTTA++ V
Sbjct: 1415 LQRAAEKSSKHGAEDKANSIITAMKERMKQREREKPEIFELIRAVFGVEDKSHAGHMKAVKQSVLDGTSKWSAKIAITVKSAQGLIAKDKSGTSDPYVTVQVGKVKKRTRTMPQELNPVWDEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEQNSGVVSLPQAKGDDAWKVYFEEAGEEIVDEFAMRYGIESIYQAMTHFHCLSTKYLCPGVPAVMSMLLANINAYYAHTTASSAV 1727          

BLAST of EMLSAG00000007474 vs. nr
Match: gi|1069817549|ref|XP_018331999.1| (PREDICTED: protein unc-13 homolog A isoform X6 [Agrilus planipennis])

HSP 1 Score: 982.245 bits (2538), Expect = 0.000e+0
Identity = 467/676 (69.08%), Postives = 553/676 (81.80%), Query Frame = 0

Query:  377 VDPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ----GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGED 1026
             DPNE KRD ED GPRLD ++FWHKLIALI SVIEEDKNSYGPVLNQFPQELN+GQLSAAT+W LFAVD KYALEEHEQ RLCKSS Y+N HF+VKWL+  YV  VPPYKG VPEYP+WFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQK++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYMKRFAKTIVKVL AYA+I+K +F  H+K+E  AC+LMNNIQQ R+QLEK + +MGG++LEEDAA++L +LQ +LNG LDELA LFA S   R+  SV  +G LL  +KGGGQ     Q   ++ V   AD +L+PLMDLLDGSLS+YA+ C+K+VLKRLLKELWKIVM+ LEK +VLPP TDK+   K+L  + KN+A  T K+ED++  FK+   GKQDVK++ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK ++++KS EL+SL++ALSLYTQTTD LIKT+V+SQ ++D++ S+EG VGEVS+QVDLFTHPGTGEHKVTVKV+                     LIGPHLSDKKR   TKSK  NWSPKYNE F FIIGNE+Q+  YELHICVKDYCF R+DRL+GV+V+QLKDI D+GSCACWLSLG+RIQMDETGWTILRILSQRTNDEVAKEFVKLKS++R E+
Sbjct: 2057 ADPNEPKRDPEDHGPRLDSVDFWHKLIALIVSVIEEDKNSYGPVLNQFPQELNIGQLSAATMWSLFAVDMKYALEEHEQHRLCKSSAYMNLHFKVKWLYTNYVKDVPPYKGAVPEYPSWFEPFVMQWLNENDDVSLEYLHGAFNRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMKRFAKTIVKVLIAYADIVKREFPEHLKEERIACILMNNIQQLRVQLEKMFESMGGEKLEEDAANILKELQQSLNGALDELASLFASSLEPRITQSVKELGDLLLAIKGGGQPGTLNQPAQRNAVAVEADEVLRPLMDLLDGSLSLYAQSCEKTVLKRLLKELWKIVMRILEKTVVLPPMTDKTMVFKSLTDNAKNLAANT-KIEDMSRIFKNHMTGKQDVKNA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKAFLDKSSELQSLRYALSLYTQTTDTLIKTFVTSQVNEDTTDSQEGCVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWTVVSGMFRPFVEVNLIGPHLSDKKRKHATKSKANNWSPKYNETFHFIIGNEEQLDFYELHICVKDYCFAREDRLVGVAVMQLKDIVDRGSCACWLSLGKRIQMDETGWTILRILSQRTNDEVAKEFVKLKSDIRQEN 2730          

HSP 2 Score: 559.296 bits (1440), Expect = 1.044e-168
Identity = 256/313 (81.79%), Postives = 287/313 (91.69%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+DKAN+II AMKERMK+RERE+PEIFELIR +F +   +H+  M   +Q+VLDGTSKWS KIA TV CAQGLIAKDK+GTSDPYVTVQVGK KKRT+T+PQELNP W+EKFYFECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G V+LP+A+G+DAWK+YFDE  +EIVDEFA+RYG+ESI+QAMTHFHCLS+KYLCPGVPA MSTLLANINA+YAHTTA++ V
Sbjct: 1637 LQRAAEKSSKHGAEDKANSIITAMKERMKQREREKPEIFELIRAVFGVEDKSHVGHMKAVKQSVLDGTSKWSAKIAITVKCAQGLIAKDKSGTSDPYVTVQVGKVKKRTRTMPQELNPVWDEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEQNSGVVNLPQAKGDDAWKVYFDEAPEEIVDEFAMRYGIESIYQAMTHFHCLSTKYLCPGVPAVMSTLLANINAYYAHTTASSAV 1949          

BLAST of EMLSAG00000007474 vs. nr
Match: gi|1008456649|ref|XP_015840296.1| (PREDICTED: protein unc-13 homolog B isoform X9 [Tribolium castaneum])

HSP 1 Score: 982.245 bits (2538), Expect = 0.000e+0
Identity = 469/679 (69.07%), Postives = 555/679 (81.74%), Query Frame = 0

Query:  378 DPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ---GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQGQ 1031
            DPNE KRD ED GPRLD ++FWHKLIALI SVIEEDKNSYGPVLNQFPQELN+GQLSAAT+W LFAVD KYALEEHEQ RLCKSS Y+N HF+VKWL+  YV  VPPYKG VPEYPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQ+++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYMKRFAKTIVKVL AYA+I+KG+F  HVK+E  AC+LMNNIQQ R+QLEK + +MGG++LEEDAA++L  LQ TLNG LDELA  FA S   R+  SV  +G LL  +KGGGQ    Q   ++ V + AD +L+PLMDLLDGSLS+YA+ C+K+VLKRLLKELWKIVM+ LEK +VLPP TDK+   K+L  + KN+A KT K+ED++  FK+   GKQDVK++ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK ++++KS EL+SL++ALSLYTQTTD LIKT+V++Q ++D+   +EGSVGEVS+QVDLFTHPGTGEHKVTVKV+                     LIGPHLSDKKR   TKSK  NWSPKYNE F F IGNE+Q+  +ELHICVKDYCF R+DRL+GV+V+QLKDI ++GSCACWLSLG+RIQMDETGWTILRILSQRTNDEVAKEFVKLKS++R E+PL  Q
Sbjct: 1833 DPNEPKRDPEDHGPRLDSVDFWHKLIALIVSVIEEDKNSYGPVLNQFPQELNIGQLSAATMWSLFAVDMKYALEEHEQHRLCKSSAYMNLHFKVKWLYSNYVKDVPPYKGAVPEYPAWFEPFVMQWLNENDDVSLEYLHGAFNRDKKDGFQRSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMKRFAKTIVKVLIAYADIVKGEFPEHVKEERIACILMNNIQQLRVQLEKMFESMGGEKLEEDAANILKDLQQTLNGCLDELATQFASSLEPRITVSVKELGDLLLSIKGGGQPGTTQPAQRNVVASEADEVLRPLMDLLDGSLSLYAQSCEKTVLKRLLKELWKIVMRILEKTVVLPPMTDKTMMFKSLTDNAKNLAAKT-KIEDMSTLFKNHMAGKQDVKNA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKAFLDKSPELQSLRYALSLYTQTTDTLIKTFVTTQINEDTLDGQEGSVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWNVVSGMFRPFVEVNLIGPHLSDKKRKHATKSKANNWSPKYNETFHFTIGNEEQLDYFELHICVKDYCFAREDRLVGVAVMQLKDIVEKGSCACWLSLGKRIQMDETGWTILRILSQRTNDEVAKEFVKLKSDIRQENPLPNQ 2509          

HSP 2 Score: 561.992 bits (1447), Expect = 5.110e-170
Identity = 257/313 (82.11%), Postives = 287/313 (91.69%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+DKAN+II AMKERMK+RERE+PEIFELIR  FS+ PD HI+ + Q EQ  ++GTSKWSCKIA TV  AQGLIAKDK+GTSDPYVTVQVGK KKRT+T+PQELNP W+EKFYFECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G VSLP+A+G+DAWK+YF+E  +EIVDEFA+RYG+ESI+QAMTHFHCLS+KYLCPGVPA MS LLANINA+YAHTTA++ V
Sbjct: 1412 LQRAAEKSSKHGAEDKANSIITAMKERMKQREREKPEIFELIRTTFSVDPDTHIDSIEQAEQMTIEGTSKWSCKIAITVKSAQGLIAKDKSGTSDPYVTVQVGKVKKRTRTMPQELNPVWDEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEQNSGVVSLPQAKGDDAWKVYFEEAGEEIVDEFAMRYGIESIYQAMTHFHCLSTKYLCPGVPAVMSMLLANINAYYAHTTASSAV 1724          

HSP 3 Score: 71.633 bits (174), Expect = 7.160e-9
Identity = 40/80 (50.00%), Postives = 45/80 (56.25%), Query Frame = 0

Query:   15 RSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVS-------------------------ELKLHVYKKXLQALIYPISSAA 69
            R+N    DNPF+ +IDSMP + RPRRKSIPLVS                         ELK+HVYKK LQALIYPISS  
Sbjct: 1282 RTNGHPGDNPFYSNIDSMP-DIRPRRKSIPLVSDLTMAATKRNAGLTSAVPRATLNDEELKMHVYKKTLQALIYPISSTT 1360          

BLAST of EMLSAG00000007474 vs. nr
Match: gi|1008456647|ref|XP_015840295.1| (PREDICTED: protein unc-13 homolog B isoform X8 [Tribolium castaneum])

HSP 1 Score: 982.245 bits (2538), Expect = 0.000e+0
Identity = 469/679 (69.07%), Postives = 555/679 (81.74%), Query Frame = 0

Query:  378 DPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ---GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQGQ 1031
            DPNE KRD ED GPRLD ++FWHKLIALI SVIEEDKNSYGPVLNQFPQELN+GQLSAAT+W LFAVD KYALEEHEQ RLCKSS Y+N HF+VKWL+  YV  VPPYKG VPEYPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQ+++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYMKRFAKTIVKVL AYA+I+KG+F  HVK+E  AC+LMNNIQQ R+QLEK + +MGG++LEEDAA++L  LQ TLNG LDELA  FA S   R+  SV  +G LL  +KGGGQ    Q   ++ V + AD +L+PLMDLLDGSLS+YA+ C+K+VLKRLLKELWKIVM+ LEK +VLPP TDK+   K+L  + KN+A KT K+ED++  FK+   GKQDVK++ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK ++++KS EL+SL++ALSLYTQTTD LIKT+V++Q ++D+   +EGSVGEVS+QVDLFTHPGTGEHKVTVKV+                     LIGPHLSDKKR   TKSK  NWSPKYNE F F IGNE+Q+  +ELHICVKDYCF R+DRL+GV+V+QLKDI ++GSCACWLSLG+RIQMDETGWTILRILSQRTNDEVAKEFVKLKS++R E+PL  Q
Sbjct: 1833 DPNEPKRDPEDHGPRLDSVDFWHKLIALIVSVIEEDKNSYGPVLNQFPQELNIGQLSAATMWSLFAVDMKYALEEHEQHRLCKSSAYMNLHFKVKWLYSNYVKDVPPYKGAVPEYPAWFEPFVMQWLNENDDVSLEYLHGAFNRDKKDGFQRSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMKRFAKTIVKVLIAYADIVKGEFPEHVKEERIACILMNNIQQLRVQLEKMFESMGGEKLEEDAANILKDLQQTLNGCLDELATQFASSLEPRITVSVKELGDLLLSIKGGGQPGTTQPAQRNVVASEADEVLRPLMDLLDGSLSLYAQSCEKTVLKRLLKELWKIVMRILEKTVVLPPMTDKTMMFKSLTDNAKNLAAKT-KIEDMSTLFKNHMAGKQDVKNA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKAFLDKSPELQSLRYALSLYTQTTDTLIKTFVTTQINEDTLDGQEGSVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWNVVSGMFRPFVEVNLIGPHLSDKKRKHATKSKANNWSPKYNETFHFTIGNEEQLDYFELHICVKDYCFAREDRLVGVAVMQLKDIVEKGSCACWLSLGKRIQMDETGWTILRILSQRTNDEVAKEFVKLKSDIRQENPLPNQ 2509          

HSP 2 Score: 548.895 bits (1413), Expect = 2.110e-165
Identity = 254/313 (81.15%), Postives = 284/313 (90.73%), Query Frame = 0

Query:   65 ISSAAEKSSKHGADDKANTIIIAMKERMKERERERPEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +  AAEKSSKHGA+DKAN+II AMKERMK+RERE+PEIFELIR +F +   +H   M   +Q+VLDGTSKWS KIA TV  AQGLIAKDK+GTSDPYVTVQVGK KKRT+T+PQELNP W+EKFYFECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G VSLP+A+G+DAWK+YF+E  +EIVDEFA+RYG+ESI+QAMTHFHCLS+KYLCPGVPA MS LLANINA+YAHTTA++ V
Sbjct: 1412 LQRAAEKSSKHGAEDKANSIITAMKERMKQREREKPEIFELIRAVFGVEDKSHAGHMKAVKQSVLDGTSKWSAKIAITVKSAQGLIAKDKSGTSDPYVTVQVGKVKKRTRTMPQELNPVWDEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEQNSGVVSLPQAKGDDAWKVYFEEAGEEIVDEFAMRYGIESIYQAMTHFHCLSTKYLCPGVPAVMSMLLANINAYYAHTTASSAV 1724          

HSP 3 Score: 71.633 bits (174), Expect = 7.160e-9
Identity = 40/78 (51.28%), Postives = 45/78 (57.69%), Query Frame = 0

Query:   15 RSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVS-------------------------ELKLHVYKKXLQALIYPISS 67
            R+N    DNPF+ +IDSMP + RPRRKSIPLVS                         ELK+HVYKK LQALIYPISS
Sbjct: 1282 RTNGHPGDNPFYSNIDSMP-DIRPRRKSIPLVSDLTMAATKRNAGLTSAVPRATLNDEELKMHVYKKTLQALIYPISS 1358          

BLAST of EMLSAG00000007474 vs. nr
Match: gi|1004172941|gb|KXZ75744.1| (Protein unc-13 homolog B-like Protein [Tribolium castaneum])

HSP 1 Score: 978.393 bits (2528), Expect = 0.000e+0
Identity = 469/679 (69.07%), Postives = 555/679 (81.74%), Query Frame = 0

Query:  378 DPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQ---GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKSAAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI---------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDPLQGQ 1031
            DPNE KRD ED GPRLD ++FWHKLIALI SVIEEDKNSYGPVLNQFPQELN+GQLSAAT+W LFAVD KYALEEHEQ RLCKSS Y+N HF+VKWL+  YV  VPPYKG VPEYPAWFEPFVMQWLNENDDVSLE+LH A+ RDKKD FQ+++EH+ FSNSVVDVFTQLTQCF+V+ KLEC DPEIWKRYMKRFAKTIVKVL AYA+I+KG+F  HVK+E  AC+LMNNIQQ R+QLEK + +MGG++LEEDAA++L  LQ TLNG LDELA  FA S   R+  SV  +G LL  +KGGGQ    Q   ++ V + AD +L+PLMDLLDGSLS+YA+ C+K+VLKRLLKELWKIVM+ LEK +VLPP TDK+   K+L  + KN+A KT K+ED++  FK+   GKQDVK++ +S V DISKE E++LSPKQC+VL+ AL+ IKQYFHAGG GLK ++++KS EL+SL++ALSLYTQTTD LIKT+V++Q ++D+   +EGSVGEVS+QVDLFTHPGTGEHKVTVKV+                     LIGPHLSDKKR   TKSK  NWSPKYNE F F IGNE+Q+  +ELHICVKDYCF R+DRL+GV+V+QLKDI ++GSCACWLSLG+RIQMDETGWTILRILSQRTNDEVAKEFVKLKS++R E+PL  Q
Sbjct:  598 DPNEPKRDPEDHGPRLDSVDFWHKLIALIVSVIEEDKNSYGPVLNQFPQELNIGQLSAATMWSLFAVDMKYALEEHEQHRLCKSSAYMNLHFKVKWLYSNYVKDVPPYKGAVPEYPAWFEPFVMQWLNENDDVSLEYLHGAFNRDKKDGFQRSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMKRFAKTIVKVLIAYADIVKGEFPEHVKEERIACILMNNIQQLRVQLEKMFESMGGEKLEEDAANILKDLQQTLNGCLDELATQFASSLEPRITVSVKELGDLLLSIKGGGQPGTTQPAQRNVVASEADEVLRPLMDLLDGSLSLYAQSCEKTVLKRLLKELWKIVMRILEKTVVLPPMTDKTMMFKSLTDNAKNLAAKT-KIEDMSTLFKNHMAGKQDVKNA-LSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKAFLDKSPELQSLRYALSLYTQTTDTLIKTFVTTQINEDTLDGQEGSVGEVSIQVDLFTHPGTGEHKVTVKVVAANDLKWNVVSGMFRPFVEVNLIGPHLSDKKRKHATKSKANNWSPKYNETFHFTIGNEEQLDYFELHICVKDYCFAREDRLVGVAVMQLKDIVEKGSCACWLSLGKRIQMDETGWTILRILSQRTNDEVAKEFVKLKSDIRQENPLPNQ 1274          

HSP 2 Score: 490.73 bits (1262), Expect = 8.903e-151
Identity = 223/275 (81.09%), Postives = 250/275 (90.91%), Query Frame = 0

Query:  107 RNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNVDPNE 381
            R  FS+ PD HI+ + Q EQ  ++GTSKWSCKIA TV  AQGLIAKDK+GTSDPYVTVQVGK KKRT+T+PQELNP W+EKFYFECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKR+DKS+VSGAIRL ISVEIKGEEKVAPYHVQYTCLHENLFHYLCE+N G VSLP+A+G+DAWK+YF+E  +EIVDEFA+RYG+ESI+QAMTHFHCLS+KYLCPGVPA MS LLANINA+YAHTTA++ V  ++
Sbjct:  219 RTTFSVDPDTHIDSIEQAEQMTIEGTSKWSCKIAITVKSAQGLIAKDKSGTSDPYVTVQVGKVKKRTRTMPQELNPVWDEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEQNSGVVSLPQAKGDDAWKVYFEEAGEEIVDEFAMRYGIESIYQAMTHFHCLSTKYLCPGVPAVMSMLLANINAYYAHTTASSAVSASD 493          

BLAST of EMLSAG00000007474 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold131_size323982-processed-gene-0.13 (protein:Tk05544 transcript:snap_masked-scaffold131_size323982-processed-gene-0.13-mRNA-1 annotation:"protein unc-13 homolog a-like isoform x11")

HSP 1 Score: 1159.44 bits (2998), Expect = 0.000e+0
Identity = 557/677 (82.27%), Postives = 604/677 (89.22%), Query Frame = 0

Query:  378 DPNEAKRDSEDTGPRLDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNVGQLSAATIWGLFAVDKKYALEEHEQSRLCKSSTYLNCHFRVKWLHKTYVSSVPPYKGQVPEYPAWFEPFVMQWLNENDDVSLEFLHSAYARDKKDKFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTIVKVLTAYANILKGDFHNHVKDEPTACVLMNNIQQTRIQLEKTYNAMGGQELEEDAASVLNQLQSTLNGVLDELAHLFAMSFNRRVDASVISMGKLLWEVKGGGQGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMKSLEKNIVLPPTTDKS------AAIKNLAGDVKNMAEKTKKVEDVTGFFKS-FGGKQDVKSSVMSVVSDISKEGERSLSPKQCSVLEAALEVIKQYFHAGGIGLKMSYVNKSEELKSLQHALSLYTQTTDALIKTYVSSQTHQDSSSSEEGSVGEVSVQVDLFTHPGTGEHKVTVKVI--------------------LIGPHLSDKKRLQKTKSKNANWSPKYNEIFSFIIGNEDQIGSYELHICVKDYCFGRDDRLIGVSVVQLKDIQDQGSCACWLSLGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKSEVRGEDP 1027
            DP  AK+D +++GPR DDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELN+G LSAATIW LFA+D KYALE+H   R CKSSTYLNCHFRVKWL+KTYV+ VPPYKGQVPEYPAWFEPFV+QWL+END+VSLEFLHSAYARDKKD+FQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKT+VKVLT+YAN+LK DF NHVKDE TACVLMNNIQQTRIQLEKTY AMGGQELEEDAASVLNQLQS LN  LDELA +FA SFNRRV  SV++MGKLL+EVKGGG GQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVM+SLE+NIVLPP  DK+      A IKNLAGDVKN+AE TKKVEDVTGFFKS F GK DVK  +++VVSD+ +   RSLSPKQC+VLEAALE IKQYFHAGG+GLKM+YVNKSE+LKSLQHALSLYTQTTDALIK +V++Q +QD+SSSEEGS GEVSVQVDL+THPGTGEHKV+VKV+                    LIGPHLSDKKR Q TK+KNANWSPKYNE F+FIIGNE+ +GSYELHI VKDYCFGRDDRLIGVSV+QLKDIQDQGSCACWL+LGRRIQMDETGWTILRILSQRTNDEVAKEFVKLK EVRGEDP
Sbjct: 2046 DP--AKKDGDESGPRKDDLEFWHKLIALITSVIEEDKNSYGPVLNQFPQELNIGHLSAATIWNLFAMDMKYALEDHAHERACKSSTYLNCHFRVKWLYKTYVAHVPPYKGQVPEYPAWFEPFVLQWLSENDEVSLEFLHSAYARDKKDRFQKNTEHSNFSNSVVDVFTQLTQCFEVLQKLECQDPEIWKRYMKRFAKTVVKVLTSYANLLKQDFPNHVKDEATACVLMNNIQQTRIQLEKTYQAMGGQELEEDAASVLNQLQSKLNKDLDELAQIFAGSFNRRVGVSVMAMGKLLYEVKGGGHGQAISKSEVQAAADGILQPLMDLLDGSLSMYAEICDKSVLKRLLKELWKIVMRSLERNIVLPPNNDKAVMMASEAVIKNLAGDVKNIAENTKKVEDVTGFFKSKFSGKGDVK-DMLNVVSDMKEGQSRSLSPKQCAVLEAALETIKQYFHAGGVGLKMNYVNKSEDLKSLQHALSLYTQTTDALIKNFVATQNNQDTSSSEEGSAGEVSVQVDLYTHPGTGEHKVSVKVVAANDLKWPKTQTFKPFVEVNLIGPHLSDKKRRQVTKTKNANWSPKYNESFNFIIGNEESMGSYELHIAVKDYCFGRDDRLIGVSVMQLKDIQDQGSCACWLALGRRIQMDETGWTILRILSQRTNDEVAKEFVKLKYEVRGEDP 2719          

HSP 2 Score: 600.512 bits (1547), Expect = 0.000e+0
Identity = 299/451 (66.30%), Postives = 330/451 (73.17%), Query Frame = 0

Query:    8 NEKTGGNRSNRAESDNPFFKDIDSMPPEFRPRRKSIPLVSEL-------------------------KLHVYKKXLQALIYPISSAA----------------------------------------------------EKSSKHGADDKANTIIIAMKERMKERERER----PEIFELIRNIFSISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAPYHVQYTCLHENLFHYLCEKNGGNVSLPEARGEDAWKIYFDENEQEIVDEFALRYGVESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATTNV 377
            +EK G  ++++  +DNPFFKDIDSM  E+RPRRKSIPLVSEL                         K+HVY+K LQALIYPISS                                                      +++++  A   A+    A+   MKER + R    PE+FELIR  F +  D H++LM+Q EQ VLDGTSKWSCKIA TVICAQGLIAKDKTGTSDPY TVQVGKSKKRTKTVP+ELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQK TRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSG+IRLQISVEIKGEEKVAPYHVQYTCLHENLFH+LCE+NGG+V LPEA GEDAWKIYF+  +QEI+DEFALRYG+ESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTAT NV
Sbjct: 1484 SEKRGDGQASKPGADNPFFKDIDSMD-EYRPRRKSIPLVSELTMAAKRNATGLASAMPRQSLNDEELKMHVYRKTLQALIYPISSTTPHNFVLWSATSPTYCYECEGLLWGIARQGVRCTECGVKCHEKCKDLLNADCLQRAAEKSAKHGADDKANAIIVAMKERMKTRVEVKPEVFELIRTTFDVKADTHLDLMHQAEQAVLDGTSKWSCKIAITVICAQGLIAKDKTGTSDPYTTVQVGKSKKRTKTVPKELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGSIRLQISVEIKGEEKVAPYHVQYTCLHENLFHFLCEQNGGDVKLPEAHGEDAWKIYFEGPQQEIIDEFALRYGIESIFQAMTHFHCLSSKYLCPGVPAQMSTLLANINAFYAHTTATNNV 1933          

BLAST of EMLSAG00000007474 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold252_size238019-snap-gene-1.36 (protein:Tk06203 transcript:maker-scaffold252_size238019-snap-gene-1.36-mRNA-1 annotation:"multiple c2 and transmembrane domain-containing protein partial")

HSP 1 Score: 71.2478 bits (173), Expect = 1.170e-12
Identity = 42/146 (28.77%), Postives = 70/146 (47.95%), Query Frame = 0

Query:  133 SKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFEC-HNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGE--MDVWYNLEKRSDKSSVSGAIRLQISVEIKGEEKVAP 275
            S WS  +   +I A+ L+A D  GTSDPY   ++G  + ++K + + LNP W E F      + +  +++ VWD+D            R  DDF+G+  +++  L  +   D WY+LE         G+ R+ + + I G     P
Sbjct:  444 SAWSAVVNVGLIEAKDLLAMDFEGTSDPYCKFRLGNERYKSKIIYETLNPTWLESFDLNVFEDQAQELEITVWDKDQ-----------RSKDDFIGRCSVDLSLLERDCTHDQWYDLE--------DGSGRIHLLISISGLTNAEP 570          

HSP 2 Score: 56.225 bits (134), Expect = 5.239e-8
Identity = 31/120 (25.83%), Postives = 54/120 (45.00%), Query Frame = 0

Query:  139 IACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGA 258
            +A  + CA+GL A D  G SDPYV +++   + +T T  + ++P WN  F     +    + + V DED +            + +FLG+  I +  +      WY L+ +  +    G 
Sbjct:  604 LAVKIFCAKGLYAADLNGKSDPYVVLELDNERIQTHTEYKTISPTWNRIFLMPIKDIHSVLYITVNDEDRN-----------HTFEFLGKLAIPMLKVESRRKKWYALKDKKLRFRAKGT 712          

HSP 3 Score: 52.373 bits (124), Expect = 6.524e-7
Identity = 38/129 (29.46%), Postives = 59/129 (45.74%), Query Frame = 0

Query:  111 SISPDNHIELMNQGEQTVLDGTSKWSCKIACTVICAQG--LIAKDKTGTSDPYVTVQV-GKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTL 236
            S+S D+HI L+                     V   QG  L A+D  GTSDPYV   + GK   ++KT+ ++LNP W+E+F     +    + ++V+D D   +           DDF+G+  I +  L
Sbjct:  269 SMSDDHHIALLRS------------HAFFHLYVHLKQGYDLAARDSNGTSDPYVKFLMKGKVVHKSKTIYKDLNPYWDEQFVLNIEDPFVPLHIKVYDYDWAFR-----------DDFMGEASISLVRL 374          

BLAST of EMLSAG00000007474 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold609_size125094-snap-gene-0.21 (protein:Tk01017 transcript:maker-scaffold609_size125094-snap-gene-0.21-mRNA-1 annotation:"multiple c2 and transmembrane domain-containing protein 1-like")

HSP 1 Score: 68.9366 bits (167), Expect = 6.214e-12
Identity = 43/137 (31.39%), Postives = 73/137 (53.28%), Query Frame = 0

Query:  135 WSCKIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSD-RIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSGAIRLQISVEIKGE 270
            WS  +   ++ A+ LIA D   +SDPY  + +GK + +TKT+   +NP+W E F    +   D ++++ +WD+  DL SK         DDF+G+  I++R L  E  V +NL +  +    +  + + IS   KG+
Sbjct:  419 WSAVVHVVLVQARDLIAMDVGDSSDPYCKMSLGKERHKTKTISNTVNPKWREGFDLYWYEEFDNQMEITIWDK--DLGSK---------DDFMGRVSIDLRDLKKE--VTHNLWREVENGKGTMNLLVTISGTTKGD 542          

HSP 2 Score: 63.5438 bits (153), Expect = 2.854e-10
Identity = 38/119 (31.93%), Postives = 60/119 (50.42%), Query Frame = 0

Query:  139 IACTVICAQGLIAKDKTGTSDPYVTVQVGKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRSDKSSVSG 257
            +   V  AQGL A D  G SDP+V +++  S+ +T T  + L P W++ F FE  +  D ++V V+DED D K            +FLG+  I +  +      WY+L+ +  + S  G
Sbjct:  580 LMVKVFKAQGLHAADLGGKSDPFVVLELVNSRVQTNTEYKTLGPTWSKLFQFEVKDIHDFLEVTVYDEDKDHKY-----------EFLGRVRIPLLKIKNNEKRWYSLKDKKLRKSAKG 687          

HSP 3 Score: 56.6102 bits (135), Expect = 4.006e-8
Identity = 41/142 (28.87%), Postives = 71/142 (50.00%), Query Frame = 0

Query:  138 KIACTVICAQGLIAKDKTGTSDPYVTVQVGKSKK-RTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTL-------------SGEMDVWYNLEKRSDKSSVSGAIRLQISV 265
            KI C +   +GLIA D+ GTSDPYV    G+ K  +T+ V +  +P W E F     N    +  +++D+D              +DDF+G+T++++ +L             SG+ +   +L +++ K    GAI ++IS+
Sbjct:  264 KIHCHLNSGKGLIAMDRGGTSDPYVKCIHGQEKLFQTRVVQKTTHPVWKENFISYVDNPFKPLTFQIYDKD-----------LVGTDDFMGETMLDLTSLQMGKVIPLVLDLMSGDNE---DLLRKNKKKKPLGAISIKISL 391          

BLAST of EMLSAG00000007474 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold186_size273091-processed-gene-0.4 (protein:Tk00902 transcript:snap_masked-scaffold186_size273091-processed-gene-0.4-mRNA-1 annotation:"e3 ubiquitin-protein ligase nedd-4")

HSP 1 Score: 63.1586 bits (152), Expect = 3.952e-10
Identity = 45/132 (34.09%), Postives = 65/132 (49.24%), Query Frame = 0

Query:  143 VICAQGLIAKDKTGTSDPYVTVQVGKSKKR--------TKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTL--SGEM-DVWYNLEKRSDKSSVSGAIRLQI 263
            +I    L  KD  G SDPYVTV + +            T  V + LNP W  +F F  +     +  +V+DE+         + TR  DDFLG  I++++ +  +G + D +Y L KRS KS V G + LQI
Sbjct:  820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDEN---------RITR--DDFLGLVIVDLKDVATTGHLPDTFYTLRKRSPKSKVRGRLFLQI 940          

HSP 2 Score: 55.8398 bits (133), Expect = 6.280e-8
Identity = 45/150 (30.00%), Postives = 68/150 (45.33%), Query Frame = 0

Query:  131 GTSKWSCKIACTVICAQGLIAKDKTGTSDPYVTVQV-------------GKSKKRTKTVPQELNPEWNEKFYFECHNSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEVRTLSGEMD------VWYNLEKRSDKSSVSGAIRL 261
            G++  +  +   VI   GL  KD  G SDPY+ + +              K+KKRT      LNP+W E+F F    S+ ++ + V+DE+         + TR  DDFLG   + +  L  E +        Y L  RS +S V G ++L
Sbjct:   14 GSTDRTKLLRLKVIAGHGLAKKDIFGASDPYLRIDLVTVDGEEVEDSVLTKTKKRT------LNPKWEEEFIFRVKPSAHKLVLEVFDEN---------RLTR--DDFLGMVELTLANLPRESEGRNIPTKQYLLRPRSARSKVKGNLQL 146          

BLAST of EMLSAG00000007474 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold1589_size34815-snap-gene-0.7 (protein:Tk01204 transcript:maker-scaffold1589_size34815-snap-gene-0.7-mRNA-1 annotation:"protein kinase brain isozyme")

HSP 1 Score: 54.6842 bits (130), Expect = 1.370e-7
Identity = 36/117 (30.77%), Postives = 56/117 (47.86%), Query Frame = 0

Query:  138 KIACTVICAQGLIAKDKTGTSDPYVTVQV----GKS-KKRTKTVPQELNPEWNEKFYFECH--NSSDRIKVRVWDEDNDLKSKLRQKFTRESDDFLGQTIIEV-RTLSGEMDVWYNL 246
            K+ C V+ A+ LI  D  G SDPYV +++    G++ KK++KT+   LNP WNE    +    +   R+ V VWD D   +           +DF+G     +   +    D W+ L
Sbjct:  173 KLVCEVVEAKNLIPMDPNGLSDPYVKIKLIPDQGENLKKKSKTIKANLNPTWNETLSIDLKPDDKDRRLLVEVWDWDRTSR-----------NDFMGSMSFGISEIIKNPQDGWFKL 278