BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000007725 (supercontig:LSalAtl2s:LSalAtl2s44:25674:28922:-1 gene:EMLSAG00000007725 transcript:EMLSAT00000007725 description:"maker-LSalAtl2s44-augustus-gene-0.13")

HSP 1 Score: 851.277 bits (2198), Expect = 0.000e+0
Identity = 450/997 (45.14%), Postives = 632/997 (63.39%), Query Frame = 0

Query:   29 MIPRPSSVHVENEPSVY--SQP----------VYLKSGRNPCLKCLDAMKNGIISGMERFFFFYGKAVANRPGWFIFLCILIVSASGSGLMKFYSENVGHKLWISEASSSRKNVDWLWENYPPSFRFASLIFEAEDILVPEVIQSMYSIHKEIEAIKTVHGEGWRDNCQMVPVFKAPDISQILSFGRRKRDTQNITEPADGFETSFQDDFFSEDDGFGNVGLDDIPANTTDETNGMLDMAEAFSADSYPQPYCDIVEGIETACFEMSILELWANDGSYDDQTDSDIASLTKRSIIDKLNQVNTSGVFLIQRNFSSFLSEITYDPTNGQIIAARATVMRWFGRMNGTEALHNSVKERNEPITHGTLEFEGEMLKIMLNETGYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNA--------LSQKDYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFP-FGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQF---EFKKPLQCGQEASQLMLSK-----INFVHKVF-SGPKEHIPAMNRIKELI--AQVNASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVE-HGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGP 992
            MIPRP+S++   + S    SQP          V  + G +   KC     N I+ G+E +F ++G+ +   P + I LC   + AS +G   +  E    KLW S+ + SR N ++L E +P  FRFA++I EA+++L P  I +M+ + K+I  IKT +G+  R  C  +PV K P+    +   RRKR   +     D F + ++ +FFS+         +D      D+    +   E  S ++YP+PYC+IV+ +E  CFE S+LELW ++G Y+  T+  + SL+   I+D +N  N SG+FLI++NF+  L  IT DP   +I+ A+AT M WF +MN  EA  + V+ +   +   T+EFE EML I+LN+TG P+GLK   +  RSF D+AG TILGDV  LGIGY+IVFF+V++MLG+F+ V  R +L+ AGI+ V  GI+ + G+CSA+G F+ PMH ILPFLLLGIGIDDMF+I+QC+NTL   EK+   +DKFG TM+HAGVAIT+TS+TD++AFGIGG++ +PAL+SFC++++VGI A Y++Q TFFVAWM  D++RI + R+GCCPC        N         +     +  +F      L   P+K L+I++T+  T +  +GN L+EQ+FD  WF+PP TY++ WF  N ++F   GG  V +Y + VD   D   +  ++  I+ Q+DI+D V SWT   TNY+ KY D         +     +++QF  SP G +Y+  F          CG+ ++    S       NF +KV  S  K  I AMNRIK+++  A + +SGRIFP+SQGY++WETDEII  ELY+N+  A + VFI T  +ISN + SFLVL  V+L+++DV G MHFW LTIDTVS N LII+IGLCVDYS H+AHRF++E  G+R+QR+ + L NIGPAV+NGG STFLAF LL  S SHVF +FFK+FFLVVTFGLFHGLVFLPVILS++GP
Sbjct:    1 MIPRPNSIYSNTDHSNTGTSQPFLASSANHHDVVERQGGSKTSKC-KCQTNCIVRGLESYFQWHGRFIGKHPVFVICLCFAFLLASFAGFPYYRKEERRIKLWSSDKTPSRLNHEFLHEYFPSDFRFANVIVEADNVLDPAAIXAMFQMRKKISEIKTNYGDTLRTKCAKMPVVKTPNFLSFIFGKRRKRRVAD-----DFFTSDYESNFFSDSFEHEMEEEEDNSGFDKDD----ISYPERLSVEAYPEPYCNIVDSMEETCFESSVLELWGDNGEYE-STEKRLESLSLIDILDTINNKNRSGIFLIKKNFTHMLGGITRDPITKEIVGAKATRMFWFLKMNIAEARKSPVEGQGVIVDKKTMEFEKEMLDILLNDTGLPEGLKISPHVSRSFFDIAGPTILGDVKILGIGYSIVFFYVLLMLGKFDWVGXRXYLAIAGIISVVFGIVFSIGICSAMGFFWSPMHSILPFLLLGIGIDDMFIIIQCWNTLNPYEKASSSVDKFGYTMRHAGVAITVTSLTDIVAFGIGGTSXLPALRSFCIFSAVGIAAIYYFQVTFFVAWMVFDEKRISSGRNGCCPCIEVKKVKKNTEEIMEDMEVESLSIMSKIFRFYASHLKFKPVKFLIILITMGFTSLGAYGNYLIEQKFDPIWFIPPHTYLSDWFEANTKWFSDKGGFFVNVYVYEVDLSVDLMELERIIRAIESQSDIIDSVNSWT---TNYI-KYVDTH----GLAEGTLYERITQFLHSPMGLQYKRNFLTVNGTADFDCGKSSTGFFNSNESIWYFNFNYKVLESDSKIKISAMNRIKDILNSANLTSSGRIFPLSQGYSMWETDEIIGYELYKNIIFAFVAVFIMTWIVISNFVASFLVLFAVMLTVLDVSGIMHFWGLTIDTVSCNFLIISIGLCVDYSTHVAHRFLIEAEGSRDQRIVKALTNIGPAVMNGGISTFLAFCLLCGSSSHVFITFFKVFFLVVTFGLFHGLVFLPVILSLIGP 978          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000003602 (supercontig:LSalAtl2s:LSalAtl2s197:152115:159124:1 gene:EMLSAG00000003602 transcript:EMLSAT00000003602 description:"maker-LSalAtl2s197-snap-gene-1.29")

HSP 1 Score: 403.29 bits (1035), Expect = 1.173e-125
Identity = 295/944 (31.25%), Postives = 475/944 (50.32%), Query Frame = 0

Query:   72 GMERFFFFYGKAVANRPGWFIFLCILIVSASGSGLMKFYSENVGHKLWISEASSSRKNVDWLWENYPPSFRFASLIFEAEDILVPEVIQSMYSIHKEIEAIKTVHGEGWRDNCQMVPVFKAPDISQILSFGRRKRDTQNITEPADGFETSFQDDFFSEDDGFGNVGLDDIPANTTDETNGMLDMAEAFSADSYPQPYCDIVEGIETACFEMSILELWANDGSYDDQTDSDIASLTKRSIIDKLNQVNTSGVFLIQRNFSSFLSEITYDPTNGQIIAARATVMRWFGRMNGTEA-----LHNSVKERNEPITHGTLEFEGEMLKIMLNETGYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTE---EEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPC-WTHSNWTPNALSQKDYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYF-PFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDD-VESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGG-KYREQFEFKKPLQCGQEASQLMLSK--INFVHKVFSGPKEHIPAMNRIKELIAQVNA--SGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFM-VEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGPGPNAVE 998
            G+   F+ YG  VA     FI L + I  AS  G + + +E+   K+W+ + S   +N   + +    +   A++I     ++V   +  + +  KE+ A+  +H          +   KAP          ++R T  I+ P                                                  P   C  +   E  C   SI E+W +D S   +  S IA LT   I+D L+Q + +   L +R F   +    ++  NG +  A+A ++ +  + N ++         SVKE     + G    E   L + L  +            R  F +     +  DV     GY IVF +++  LG  N +E + +L+  GI  VGMG  ++ G+   L ++  PM+ ILPF+LLGIGIDDMFVIVQ    + +   EE    +    G  M+ AG AIT+TS+TD++AFGIGG + +PAL++FCLY+S GI   YFY  TFF+ W  ID RR+E+ RD C PC    S+W P   ++K +   +F+  G  +     + L II+T+ ++G  ++G  L+E +FD   ++ P++ +  +F  ++++F   GG     Y  N +  +    I  + +++K    I ++ + ++   F+  +N              ++  S L+ F ++ R    +  +F+  +   CG     + LS   + F H   +  ++ I +M +I ++  ++    +G IFP +  Y+   T +II  EL RN+ LA++ VFI  LFL++  +TS  VL+ ++LS+IDV G+M+FW ++IDT SA  L +++GL VDYSAHIAH ++     +R ++V+ +L  +GPAVL+GG STFLAFILLA+S S VF++FFKIFFLVV FGL+HGL+FLPV+LS +GP    V+
Sbjct:   19 GINWIFYQYGMIVAKYSYVFIGLSLGIFIASTLGFIDYVTESDPTKIWLPQDSYYVQNTKKIKDLNSDAVNAATVI----QVIVESEVNILRA--KELNAVYKLHSS--------IINLKAPSNETFKDLCFKERVTVPISTP--------------------------------------------------PSEICRALANAEWLCSAKSIFEIWTSDES--SKEASIIAQLTDEDILDALSQ-DLAISKLFRRPFRPIIGSKMFND-NGTLKGAKALLLSYSIKTNSSDKNGIPIWEKSVKEHVFNYSFGD---ESSNLSVTLFSSSI---------FRAEFQN----GMENDVQLFIFGYLIVFVYIIFNLGEMNTLEHKFYLAVVGIACVGMGYASSVGIFQLLSVYATPMNSILPFMLLGIGIDDMFVIVQASKNVKQNDDEEGENKMDSYIGNIMKSAGXAITVTSLTDILAFGIGGFSQLPALRNFCLYSSAGIFFVYFYIMTFFLGWFVIDARRVESHRDACLPCIIKKSSWVP-CYTRKSFAEXLFSCSGVLIDSLFFRGLAIIVTIVVSGFGVYGLTLIEPKFDPKSYINPESKLLSYFKASEQHFQKLGGIPGQFYILNPN--NSLVEIEKVYEELKNSEFIEENSLRTFHKAFSKTINS-----------TTNFQYSSLTNFLYTSRVPFDWAVKFKASQKPVCGDTTEDVKLSAFVLYFQHIKKTRVQDQIQSMKKIYQIGEKIEGERNGLIFPHATQYSNLITLDIILSELTRNILLALMTVFIAALFLLAXALTSIYVLLSILLSIIDVTGYMYFWGVSIDTASAVLLTVSLGLAVDYSAHIAHGYIHSSKSSRGEKVKDSLTKVGPAVLSGGISTFLAFILLANSSSIVFTTFFKIFFLVVLFGLYHGLIFLPVLLSFIGPVYRQVQ 864          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000005221 (supercontig:LSalAtl2s:LSalAtl2s276:209809:212602:-1 gene:EMLSAG00000005221 transcript:EMLSAT00000005221 description:"maker-LSalAtl2s276-augustus-gene-2.14")

HSP 1 Score: 299.286 bits (765), Expect = 3.745e-89
Identity = 196/611 (32.08%), Postives = 333/611 (54.50%), Query Frame = 0

Query:  410 SFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIE--------AKRDGCCPCWTHSNWTPNALSQKDYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDY---IHDYESIANLVDQIKQQ----------TDIVDDVESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFS--PRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQV--NASGRIFPMSQ--GYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEH-GTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGP 992
            +F D   ++I G +   G+G+ ++  + +++   F+ V QR  L   G M +GM I +++G+   +G+ +  +H I+PFLLLGIG+DD+F+I++  + L+++EK LPL ++    +QH+GV+IT+TS+TD++AF I  +T IP L++FCL  ++GI   YF+  TFF+AW  +D+RRIE         KR    P    S WT N L +K Y+  +F AI   L  G I +LV        G  I+G   ++Q++D   ++  ++Y  K+       +P  GD V IY  N+ Y   I + + I +LV+  +              ++D  ES         N   + DF         F+  L  F F+         E+F+  + L+   ++     SK  F +           AM+ I   + ++    S  + P+S+   +  W  +++IS+EL RN+ L   C+ I    L++N+  S +VL  V +++ ++ GF +F  L I+ V++  LI+++GL +DYSAHI   + ++  G+R +R ++ L ++GPA+ +GGFSTFLAF++L  + S++F+SF  +    V FGL++ LVF+P++LS  GP
Sbjct:   28 NFQDEIVTSIDGGMYVFGVGFLLILIYSLLVTSDFHPVNQRVMLLLLGFMVIGMAIGSSFGLGFHIGIIYADLHPIIPFLLLGIGVDDLFIIIKSTDNLSQKEKELPLKERIALVVQHSGVSITVTSLTDLVAFLISSTTSIPLLRAFCLSVALGISFLYFFVVTFFIAWYTLDERRIEEGKAFLWFTKRVHSVPP-KKSTWTHN-LYKKYYVEYLF-AIPSQLILGGISLLVF-------GFGIFGTFNIQQDYDPITYMRQNSYQVKFIEAKNRLYPEFGDIVDIYVGNIPYWENIQEMKQIDSLVNNCQYVRTGSHSLYWFKHVIDVCES------EGFNCSTEFDFK----KAIVFSLALKPFIFADLKLNITILEEFQDFQDLKWHIQS-----SKSTFQYGHLESISVQTKAMDEIILGLEKIIFEDSDFVSPISRSLAFHQWTANKVISKELIRNMSLNFACITIVLFMLLANIQLSLIVLFTVAMTITEIAGFAYFVGLNIEIVTSLELILSVGLVLDYSAHIGVCYTIQKTGSRRERSQRALLDMGPAIFSGGFSTFLAFVVLFFNDSYIFTSFCHMLGFGVLFGLWNALVFMPLLLSYFGP 613          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000010036 (supercontig:LSalAtl2s:LSalAtl2s660:189355:234598:1 gene:EMLSAG00000010036 transcript:EMLSAT00000010036 description:"maker-LSalAtl2s660-augustus-gene-1.6")

HSP 1 Score: 232.646 bits (592), Expect = 4.109e-67
Identity = 155/460 (33.70%), Postives = 240/460 (52.17%), Query Frame = 0

Query:  109 FYS-ENVGHKLWISEASSSRKNVDWLWENYPPSFRFA--SLIFEAEDILVPEVIQSMYSIHKEIEAIKTVHGEGWRDNCQMVPVFKAPDISQILSFGRRKRDTQNITEPADGFETSFQDDFFSEDDGF------GNVGLDDIPANTTDET-NGMLDMAEAFSADSYPQPYCDIVEGIETACFEMSILELWANDGSYDDQTDSDIASLTKRSIIDKLNQVNTSGVFLIQRNFSSFLSEITYDPTNGQIIAARATVMRWFGRMNGTEALHNSVKERNEPITHGTLEFEGEMLKIMLNETGYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEK-------SLPLIDKFGA-TMQHAGVAITITSITDVIAFGIGGSTV 550
            FYS E    KLWI + S    N  W+ EN+P ++R    SL+  + +IL PE +  +  IH+++E I   H   W+D C     FK P  +  L   +   + +N T     F+    DD F  DD          +  DD      +E+ NG     E       P+ YCD VE +   CFE S+LE+W  D          I  LTK  I+  +N++  S V+  + N+ +++  I  + T G +++A      WF ++N  E +   +   +E +   +LE+E + + I LN +   + +  +  + RSF D+   +I  D  +L  G+ I+  + +IMLGR NCVE R F + AG+  + +G++ + G+ SA+GL + P+  +LPFL LGIGIDDMF+IVQC+  + E+ K       S+  +++  A TM+ AGV ITITS+TD+ AFGIGG TV
Sbjct:   20 FYSFEYKMEKLWIPQNSEFISNKKWIEENFPTNYRVQIISLLSLSGNILTPEALLLLLDIHEKVEQISAGHNTRWKDLC-----FKVPVANVFLERNKVLTNDKNTT-----FDRV--DDLFISDDNVVPQNEKTEIDYDDAFLEYENESKNG--SRYEKLLKILSPKVYCDFVETLNDRCFERSLLEIWKFDRR-------RIKRLTKDRIVQAINRIQNSPVYGYKTNYDNYMGSIIRNET-GHVVSASTLNSVWFTQVN-LENVSEVIGLTDEFVNKESLEWELKFVDIFLNAS--TENISAFGISARSFNDITKKSIFFDGIRLTGGFFIMCIYTVIMLGRLNCVEVRFFATIAGLFSILLGMVVSLGIISAMGLKYSPVFAMLPFLYLGIGIDDMFIIVQCWYNILEQNKKECSTDQSIKTLEEMMAETMKDAGVTITITSLTDIFAFGIGGITV 454          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000007475 (supercontig:LSalAtl2s:LSalAtl2s429:50360:104533:1 gene:EMLSAG00000007475 transcript:EMLSAT00000007475 description:"maker-LSalAtl2s429-augustus-gene-1.9")

HSP 1 Score: 209.534 bits (532), Expect = 5.754e-60
Identity = 137/430 (31.86%), Postives = 218/430 (50.70%), Query Frame = 0

Query:  550 VIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGS----VFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDV------ESWTDPFTNYM--NKYFDPDFPVLPYNQSY-FNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFK 966
            ++P L+ F +  ++GI A +  Q ++F AW  +DQ+RI   + G  PC      T    + K +  S    +  A    +     +++VII++L +    +WG   + QEFDI  F+P  +Y  +WF   +++    G +  IY   +D   D   + N VD+  Q  D++D        E W   F  Y+  +K        L  N S+ F   LS F +S  G +Y+  F F  PL+C Q A                                          P    Y++WET+EII  EL+RN+G+A + +F+     ++N++ SFLVL  VI++++D+ GF+HFW +TIDT++  N++++IGLCVDYS HIAH F+   G   +R   ++ +IG A++NGG +TF+A ILL  S+SH+F  FF+
Sbjct:    1 ILPGLRHFSITCAIGIAAVFLCQISWFSAWFYLDQKRILNNKSGFFPCCIQYEDTSERENGKCFEFSFGNKLIRAFSRTIMHRTFQVIVIIISLILFSFGVWGAFSIRQEFDIKKFMPSSSYFVQWFEIKEKHSVSKGWQSNIYLGRIDPPLDIPKL-NSVDEFLQ--DLLDSKAYIITKEVWWTDFKLYILRDKNVSHWRSTLFKNDSFLFRVHLSDFLYSSIGAQYKVNFRFDTPLRCNQPA------------------------------------------PNINIYSIWETEEIIVFELWRNMGIACIVIFVILSLFLANLLVSFLVLCNVIITVVDIVGFLHFWGITIDTIACVNIVLSIGLCVDYSIHIAHFFLCASGDAAERAASSVESIGIAIINGGTTTFMALILLIFSESHIFIYFFQ 385          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000010537 (supercontig:LSalAtl2s:LSalAtl2s6:1415448:1420769:-1 gene:EMLSAG00000010537 transcript:EMLSAT00000010537 description:"maker-LSalAtl2s6-augustus-gene-13.12")

HSP 1 Score: 195.282 bits (495), Expect = 4.261e-51
Identity = 155/604 (25.66%), Postives = 281/604 (46.52%), Query Frame = 0

Query:  426 LGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQR------------RIEAKRDGCCPCW---------THSNWTPNALSQKDYLGSVF--TAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYF---PFGGDRVIIYCHN-VDYIHDYESIANLVDQI---KQQTDIVDD--VESWTDPFTNYMNKYFDPDFPVLPYN-QSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGRIFPMSQGYTLWETDEI-ISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFM--VEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGPG 993
            L I   I+  F ++     +  + +  + FAG+    +  I  +G    +G+ F  ++   PFLLLGIG+DD FVI+  +        +L + ++ G     A V+IT+TS+TD  +F  G  T  P ++ FCLY  + +   YF+  T F A +A+  R            ++  K       W         T+     N    KD+ G VF    +G  L++   K LV++L  A   VA WG   +E+  +       D+Y  K++  +  YF    +    +I    N  D ++        ++++    + +  ++    +SW   F +Y+ +  + +  +LP + +  F   LS  + S      R    +    + G  AS+ ++   N ++      +  +  +  I    +  + S  +F     Y ++    + I+ +  + + +  L + I + FLI +++ SF V   +I   + V G+M +W + +D V+  NLI+ IG  VD+SAHI + ++   E  + ++R+R +L  +G  ++ G  ST L  + LA + S++F +FFK+ FLV+  G  HGL+ LPV+LS+ GPG
Sbjct:  276 LCINVVIMVVFSLVSCLMSDATKSKPLVGFAGLFSATIATIAGFGFACYVGVEFISLNFAAPFLLLGIGVDDTFVILSSWR---RSSSNLSIPERMGRCYSEAAVSITVTSLTDFFSFMAGVVTPFPCVRIFCLYTGISVAFIYFWHLTLFGAILAVSGRAEKNNLHGMFCIKVIPKSLAVNKSWLTRTFMTGGTNPKDPHNPDDNKDHAGMVFFRDNMGRLLSRTWFKSLVLLLFGAYIVVACWGVTNIEEGLEKQNMANYDSYSVKYYSMDDSYFREYSYTISVIITSASNERDLLYSDSKTQARIERVLYALENSXFIEKAATQSWLRDFLDYIKRNENYEDIILPVSTEMEFVDTLSNVYLSDPSNPLRLDVAYNHN-KNGIIASRFLIQGRNILNSYME--QSMVAELRDICNQFSTPDISISVF---HPYFIYIDQYLAIAPQTVQCILVTSLVMMIISFFLIPSLVCSFWVAFSIISIELGVIGYMTWWGVRLDGVALINLIMCIGFSVDFSAHICYHYITDTESSSPDERIRSSLYGLGLPIIQGATSTILGVLGLAFAPSYLFVTFFKMVFLVIVLGGLHGLIILPVLLSLFGPG 870          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000008727 (supercontig:LSalAtl2s:LSalAtl2s542:206164:232722:1 gene:EMLSAG00000008727 transcript:EMLSAT00000008727 description:"maker-LSalAtl2s542-augustus-gene-2.8")

HSP 1 Score: 180.259 bits (456), Expect = 1.296e-46
Identity = 161/609 (26.44%), Postives = 279/609 (45.81%), Query Frame = 0

Query:  426 LGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWT--------HSNW----------TP-NALSQKDYLGSVFTAI-----GHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYF---PFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDV--ESWTDPFTNYMNKYFDPDFPVLPYN-QSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGRIFPMSQGY--TLWETDEIISEELYRNLGLAVLCVFIT-------TLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMV--EHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGPG 993
            L IG  I F  +  M+   + V+ +  L   G++   M  + ++G     G+ F  ++   PFL+LGIGIDD FV++  +   T   +++P   +   T + A ++ITITSITD+++F IG  T  P+++ FCLY    +  TY +  TFF   MAI     ++ R     C T          NW          +P +  ++KD   +    I        +  G  KI V++       +A WG   +++  +       D+Y  +++     YF   P+  + V+    N   +     +  L+ ++ + +  VD V  ESW   F +Y+ +  + DF  L  + +  F S L + +       Y +   F        +   ++ S+      +  G K  I   N  K+ + ++    R   ++  Y  T++    I  ++    L   + C+ +        +L  I N + S  V   +I   + V GFM  W + +D++S  NLI+ IG  VD+SAHI++ +M   +  T  +R+R ++  +G  ++ G  ST L  + LA + S+VF +FFK+ FLV+  G  HGL+ LP++LSI GP 
Sbjct:  279 LNIGIMIAFCIITCMMT--DWVKSKPLLGLFGVISAIMAXLFSFGFIIYCGVPFIGINLAAPFLMLGIGIDDTFVMLGSWRR-TSVHETVP--ARLAQTYRDAAISITITSITDMLSFWIGIITPFPSVRIFCLYTGACVAFTYLWHITFFGGCMAIAGYAEKSNRHSLT-CLTVIPKSKAESKNWLYRIFCSGGISPDDPFNKKDNAENAIMVIFRDYVARAINNGYFKIFVLVFFTFYLVIAGWGVSNIQEGLERKKLARYDSYSVEFYEMEDTYFREYPYRVNVVLSGHFNYSNLQVQNDVEKLLKKL-ENSSFVDHVYTESWYRDFLDYVKR--NKDFAELDISTEENFISTLKEVYLGSGFTHYVKDVHF------STDNKSIIASRF-----LIQGTK--IYNTNDEKKFVQEL----RYVCLTSNYNVTVFHPYFIYFDQFLMVLPTTIQCITVAAFVMMFISLIFIPNPVCSLWVAFSIISIEVGVVGFMTLWGVGLDSISMINLIMCIGFSVDFSAHISYHYMSCEKSFTDKERIRDSMYALGLPIVQGAISTILGVVGLALAPSYVFITFFKMVFLVIVLGALHGLLVLPILLSIFGPS 861          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000012098 (supercontig:LSalAtl2s:LSalAtl2s867:155984:163315:1 gene:EMLSAG00000012098 transcript:EMLSAT00000012098 description:"maker-LSalAtl2s867-snap-gene-0.32")

HSP 1 Score: 130.568 bits (327), Expect = 5.849e-31
Identity = 135/577 (23.40%), Postives = 271/577 (46.97%), Query Frame = 0

Query:  445 NCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGS-TVIPALKSFCLYASVGIIATYFYQCTFFVAWMAI-----DQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVF-TAIGHGLTKGPIKILVIILTLAITG---VAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRV-IIYCHNVDYIHD--YESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKYFDPDFPV-----LPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGRIF-PMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQ--RVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGPGPNAVEIE 1000
            N +  + ++S  G + + + I  A+G    +G+ +  ++    F+LLG+G+DD++VI+  +   T E  S+P  ++     + A V+ITITS+T++++F +G +  V P++  FCLY+ +GI   Y +  TFF   +AI     +  R++ K+D             ++    D+L   + + + + L K   K++VI L L       + I  N   ++  D +     D++++ ++     YF     R  +++   +DY +    + I+N +++I Q +  V   E + + + +  ++  +    +     L  ++  F +KLS+        +  E   F          ++++ S+I    +      E    M  + E++        IF P+   +   +  +++   +Y ++ +  + V + T  LI N++ +  V + ++   I V GFM  + + +D +S   LI+ +G  +DYSAH+++ F V    RN   R++  L   GP ++    ST L    L    S++  SF  +  +V+  G+ HG V LPV+LS+     N  +I+
Sbjct:  290 NPILGKPYVSLVGSLNIVVAIGAAWGFLMYIGVEWISINLASVFILLGVGLDDIYVILFSWRR-TSELLSVP--ERLSLCYEEAAVSITITSLTNIVSFVMGSAFPVFPSVWIFCLYSGIGIFFIYIWTLTFFGGVLAISGYTEETERLKKKQDIDIV---------DSEIGFDFLNHFYESTLPNILNKVYAKVIVIALYLTYLTSFILIILNNT--KEGLDRSKLSRYDSFLSSYYKAEDNYFRQNPYRFQVVFTEALDYSNSSVRQQISNFINKI-QNSKYVSQNEQFKENWLHVFDQIVEKKQTIFTGWTLENSEHRFITKLSETLHRHHLPEIEENIVF------NHNKTKILASRIFLQSEKVRNSHEEKLMMQDLYEILDSAPFETIIFNPLFPFFD--QFLQVLPSTIY-SVSITAIVVILITFILIPNIVFTLWVTIALVSIEIGVLGFMVIFEINLDIISMICLIMCVGFSIDYSAHVSYHF-VSMNIRNNSIRLKSXLXAYGPPIVQSAVSTMLGVTGLFFLPSYIIRSFSLLLVIVILLGVIHGTVLLPVLLSLTFCNSNTDDIQ 841          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000004370 (supercontig:LSalAtl2s:LSalAtl2s22:157610:161978:1 gene:EMLSAG00000004370 transcript:EMLSAT00000004370 description:"maker-LSalAtl2s22-snap-gene-1.32")

HSP 1 Score: 108.997 bits (271), Expect = 2.923e-24
Identity = 138/606 (22.77%), Postives = 255/606 (42.08%), Query Frame = 0

Query:  407 ARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAF---LSFAGIMGVGMGIITAYGVCSALGLFFGPM-HGILPFLLLGIGIDDMFVIVQCF--NTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDG--CCPCWTHSNWTPNALSQKDYLGSVFTAIGHGLT-----KGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGD-RVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKY-FDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQ----LMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGRI--FPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGG--FSTFLAFILLADSKSHV-FSSFFKIFFLVVTFGLFHGLVFLPVILS 988
            A  S  D   S   GDV  + I Y ++F +V   L  F    ++ F   L+ AGI  V   I++  G+   +G+    +   I+PFL+L +GID++FV+V       L+ E + + +    G T++    A+ I+++T   AF +G  + +PA+ +F LYA   ++  +  Q T F+A +++D +RI++K+    CC          N  S+ +Y  ++ T     ++     K  +K  V++L      + +   + +    D    +P  +Y+ K+F   K Y+  G     ++   N+D           +  +K Q  I          F   M  +   PD   L                +  G  + + + F     C    SQ    ++ S     H V     + + A+   + L  +      I  FP S  +  +E    +  +   ++   +L + +   FL   ++     +  V   LI V GF+    + ++ + A NL++A G+ V+    I +   V  G R+    + + N    +L  G   +  +   +L  S+S++ F  FF ++  +V  G  HGL+ LPV+LS
Sbjct:  389 AEMSIQDAIESNSHGDVLVVIISYFLMFVYVSFALTDFR--NKKYFGITLAIAGIFMVLGSILSTTGLFGFIGVKATMIIFEIVPFLVLAVGIDNIFVLVLTARRKLLSLEGREMEVDSLIGITLEEIAPAMLISTLTQTCAFYLGTLSKMPAVVTFALYAGTSLLINFLAQITGFIAILSLDLKRIKSKKLDLFCCLSDKGEIDVDNPKSRFNYQKTISTFFEKYVSPVLMKKLTVKFSVMVLIAITLSITLVFTLNIPVGLDPESTMPKGSYVKKYFEWLKLYYSVGPPVYFVVNSTNMD-----------LATVKSQNRICGSAGCDPYSFQTAMKLWSTKPDLTTL----------------ATSGQSWIDDY-FSWSNDCCMVDSQGKICILFSNFMAFHTVLRTSNDFVQAIESTRILSDKFARENNITSFPYSIFHVYYEQYLSMKTDTLVSIAYVLLTMLLICWFLSGKIVIGLYTVTFVSSILIHVTGFISMLGIQLNAILAVNLLVACGISVEIC--IGYLTSVIMGKRSA---EEVYNDQAGILMSGIHITNLIGVSVLGFSQSNIFFYYFFTVYISIVVIGFVHGLIVLPVLLS 959          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000001950 (supercontig:LSalAtl2s:LSalAtl2s1345:18618:29195:1 gene:EMLSAG00000001950 transcript:EMLSAT00000001950 description:"maker-LSalAtl2s1345-snap-gene-0.7")

HSP 1 Score: 83.9593 bits (206), Expect = 1.592e-16
Identity = 77/355 (21.69%), Postives = 153/355 (43.10%), Query Frame = 0

Query:  407 ARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLF-------FGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEK--SLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDG-----CCPCWTHSNWTPNALSQKDYLGSVFTAIGH----GLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGD-RVIIYCHNVDYI----------------HDYESIANLVDQIKQQTDIVDDVESWTDPF 726
            + RS  D       GD+  + + Y I+F ++   LG  N   +R  +     +G+G  II    V +++G+F          +  I+PFL+L +G D++F++VQ   T   + +  +   ++  G  +     ++ +TS+T+   F +G  + +PA+++F LYA + ++  +  Q T F++ +++D  R   + D      CC   +  N     + Q    G V+    H     + K  ++  V+I+       ++     +E   D    +P D+++ K+F   ++Y   G     ++   NVD                      S+ NL  +  ++T I    +SW D +
Sbjct:  576 SERSIEDELAKETYGDISTIAVSYIIMFIYITFSLGE-NTTWKRFMVESKITLGLGGVIIVLLSVAASIGIFGFIDVPATLIIFEIIPFLVLAVGXDNIFIMVQ---TCQRDPRGATETHVEHVGRIVGEVAPSMLLTSVTESTCFFLGAISDMPAVRAFALYAGMALLIDFLMQVTCFISLISLDMAR---QNDNYYDIICCVRGSKKN-----VRQNGGEGVVYKLFKHFYAPFVMKEWVRAAVVIIFFGWFCTSLAVTPRIEVGLDQKLSMPDDSFVLKYFTFIEKYLSVGPPVYFVVDSSNVDLSLPGIQNKFCGGAGCSDESLNSLINLWSKQPEKTYIAAPAQSWIDDY 918          

HSP 2 Score: 57.3806 bits (137), Expect = 2.084e-8
Identity = 44/176 (25.00%), Postives = 87/176 (49.43%), Query Frame = 0

Query:  759 PRGGK--YREQFEFKKPLQCGQEA---SQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNAS-----------GRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLIS-NVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRF 917
            P+ G   Y +    K   Q   E+     ++ S     H +   PK++  A+   + L +++  S            ++FP S  Y  +E    +  +   +L ++ L + I T  L   + +TS +++V + + LI++ G M+FWN++++ VS  NL++A+G+ V++ +HIA  F
Sbjct: 1069 PKAGHAAYSQSVRLKAHPQKDNESFFQYNVIASNFMTFHTILKTPKDYYEALRWARRLSSEIENSINGNLTDKSQGVKVFPYSIFYVFYEQYLTMWSDTINSLLVSFLSISIVTFILTGFDFLTSIIIVVVIAMILINLMGMMYFWNISLNAVSLVNLVMAVGISVEFCSHIAKAF 1244          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|123778281|sp|Q0EEE2.1|PTHD3_MOUSE (RecName: Full=Patched domain-containing protein 3; AltName: Full=RND-type protein RNDEu-3)

HSP 1 Score: 212.616 bits (540), Expect = 1.015e-55
Identity = 156/592 (26.35%), Postives = 283/592 (47.80%), Query Frame = 0

Query:  428 IGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEA---------------KRDGCCPCWT-----HSNWTPNALSQKDYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRV-IIYCHNVDYIHDYESIANLVDQIKQQ---TDIVDD--VESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKV----FSGPKEHIPAMNRIKELIAQVNASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVE-HGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILS 988
            + Y ++  F ++   R +C+  +  ++  G+  V M +++ +G+   LG+ F  +    PFL+LG+G+DDMF+++  +   +  E    + ++   +     V+ITIT+IT+V+AF  G ++   +++ FC+Y    ++  YFY  T F A MA+D +R  A               K+  C P  +       +  P  L  +DY G         LT    K +V++L +     +I+G   +++  D+      D+YI  +F   ++YF   G RV +I   +V+Y  +   +   +D+   Q    + VD    E W + +  YMN   +      P +++ F + ++ F       ++   F +   +    E +    S   F+  V     S  K  +  +  I E     N    +   +Q +  ++    I E   RN+ +A   +FI +L LI + + S  V   +   ++ V GFM FWN+ +D++S  NL+I IG   D+SAHI++ F+     + N++  + L  +G  VL    ST +   +L+ +K+++F +FFKI FLV+ FG  HGL+F+PV L+
Sbjct:  345 LAYILIILFAVVSCSRLDCIRNKMCVAVFGVFSVAMSVVSGFGLMLHLGVPFVIIVANSPFLILGVGVDDMFIMISAWQKTSLSES---IRERLSNSYSKVAVSITITTITNVLAFYTGITSSFRSVQYFCIYTGTTLLFCYFYSITCFGAVMALDGKREVAWSRWLEKPDQKYSSLKKSCCVPFGSLIDKHGEDNHPMNLFFRDYFGPF-------LTTSKAKFIVVLLYIFYIISSIYGCFQVQEGLDLRNLASDDSYITPYFNVEEDYFSDYGPRVMVIVTESVNYWDN--DVRQKLDKCMTQFEENEYVDKNLTEFWLEAYMQYMNSSGNN-----PNDKNTFMNNIAGFL------QFFPIFTYDINISSSNEIT----SSRGFIQIVDVSSSSNKKRMLLKLRSIAE-----NCEVPLMVYNQAFIYFDQYAAIIENTVRNVMIASTAMFIVSLLLIPHPVCSLWVTFAIASVIVGVTGFMAFWNVNLDSISMINLVICIGFSFDFSAHISYAFVSSTEPSVNKKSIEALYLLGYPVLQSAISTIIGVCVLSAAKAYIFRTFFKIMFLVMFFGAAHGLIFIPVFLT 904          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|8134591|sp|P56941.1|NPC1_PIG (RecName: Full=NPC intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1 protein; Flags: Precursor)

HSP 1 Score: 177.563 bits (449), Expect = 5.756e-44
Identity = 166/687 (24.16%), Postives = 310/687 (45.12%), Query Frame = 0

Query:  394 ETGYPDGLKTYAN--------ARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRF-NC----VEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMH----GILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVF-TAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIA--NLV--------DQIKQQ-----------------TDIVDDVESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFE-FKKPL----------QCGQEASQLMLSKINFV--------------HKVFSGPKEHIPAMNRIKELIAQVN-------ASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVG--GFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVE-HGTRNQRVRQTLRNIGPAVLNG-GFSTFLAFILLADSKSHVFSSF-FKIFFLVVTFGLFHGLVFLPVILSILGPGPNAVE 998
            E+ + + +K Y N        A RS  D        D+  + I Y I+F ++ I LG   +C    V+ +  L  AGI+ V   +  + G+ S +G+   P+      ++PFL+L +G+D++F++VQ +    E  +   L  + G  +     ++ ++S ++ +AF +GG +V+PA+ +F L+A + ++  +  Q T FV+ + +D +R E  R     C   +       + +  L   F  +    L K  ++ +VI + + +   +I     +E   D +  +P D+Y+  +F     Y   G     +    V+  H+Y S+   N+V        D + QQ                 +  +DD   W  P ++    Y   D      N S  +    +       GK R Q E F + L          +CG+       S +N +              H V     + I AM + + + + +        +S R+FP S  Y  +E    + ++   NLG+++  +F+ T+ L+   + +  V++CV +++I V   G M  W ++++ VS  NL+++ G+ V++ +HI   F +   G+R  R  + L ++G +V +G   + F   ++LA +KS +F  F F+++  +V  G  HGL+FLPV+LS +GP  N  +
Sbjct:  584 ESEFINFVKNYKNPNLTISFMAERSIEDELNRESNSDLFTILISYAIMFLYISIALGHIKSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYIGV---PLTLIVIEVIPFLVLAVGVDNIFILVQTYQR-DERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGGLSVVPAVHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNRLDVVCCVQGAEDGAGVQASESCLFRFFKNSYAPLLLKDWMRPIVIAVFVGVLSFSIAVLNKVEIGLDQSLSMPDDSYVMDYFQSLSRYLHAGPPVYFV----VEEGHNYTSLKGQNMVCGGLGCNNDSLVQQIFTAAQLDNYTRIGFAPSSWIDDYFDWIKPQSSCCRVYNSTDQFC---NASVVDPTCIRCRPLTSEGKQRPQGEDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILGNGSGVGATYFMTYHTVLQASADFIDAMQKARLIASNITRTMGLEASSYRVFPYSVFYVFYEQYLTVIDDTIFNLGVSLGAIFLVTVVLMGCELWA-TVIMCVTIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTLSTKGSRVDRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAIVLLGATHGLIFLPVLLSYIGPSINKAK 1258          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|83305902|sp|O15118.2|NPC1_HUMAN (RecName: Full=NPC intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1 protein; Flags: Precursor)

HSP 1 Score: 175.252 bits (443), Expect = 2.937e-43
Identity = 161/683 (23.57%), Postives = 311/683 (45.53%), Query Frame = 0

Query:  407 ARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRF-NC----VEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMH----GILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKR-DGCCPCWTHSNWTPNALSQKDYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIA--NLV--------DQIKQQTDIVDDVESWTD---PFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFF---------------FSPRGGKYREQFEFKKPL----------QCGQEASQLMLSKINFV---------------HKVFSGPKEHIPAMNRIKELIAQVN-------ASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVI-LSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVE-HGTRNQRVRQTLRNIGPAVLNG-GFSTFLAFILLADSKSHVFSSF-FKIFFLVVTFGLFHGLVFLPVILSILGPGPNAVEIELDEHGINVIENGRVI 1015
            A RS  D        DV  + I Y I+F ++ + LG   +C    V+ +  L  AGI+ V   +  + GV S +GL   P+      ++PFL+L +G+D++F++VQ +    E  +   L  + G  +     ++ ++S ++ +AF +G  +V+PA+ +F L+A + +   +  Q T FV+ + +D +R E  R D  C      + T    S+         +    L K  ++ +VI + + +   +I     ++   D +  +P D+Y+  +F    +Y   G     +    ++  HDY S    N+V        D + QQ      ++++T      +++++ YFD    V P +       ++  F                +P G +  +  +F + L          +CG+       S +N +               H V     + I A+ + + + + V        ++ R+FP S  Y  +E    I ++   NLG+++  +F+ T+ L+   + S +++   I + L+++ G M  W ++++ VS  NL+++ G+ V++ +HI   F V   G+R +R  + L ++G +V +G   + F   ++LA +KS +F  F F+++  +V  G  HGL+FLPV+LS +GP  N  +    E      E  R++
Sbjct:  605 AERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLVDSKVSLGIAGILIVLSSVACSLGVFSYIGL---PLTLIVIEVIPFLVLAVGVDNIFILVQAYQR-DERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGALSVMPAVHTFSLFAGLAVFIDFLLQITCFVSLLGLDIKRQEKNRLDIFCCVRGAEDGTSVQASESCLFRFFKNSYSPLLLKDWMRPIVIAIFVGVLSFSIAVLNKVDIGLDQSLSMPDDSYMVDYFKSISQYLHAGPPVYFV----LEEGHDYTSSKGQNMVCGGMGCNNDSLVQQIFNAAQLDNYTRIGFAPSSWIDDYFDW---VKPQSSCCRVDNITDQFCNASVVDPACVRCRPLTPEGKQRPQGGDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILLGHGTRVGATYFMTYHTVLQTSADFIDALKKARLIASNVTETMGINGSAYRVFPYSVFYVFYEQYLTIIDDTIFNLGVSLGAIFLVTMVLLGCELWSAVIMCATIAMVLVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTVSMKGSRVERAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKSCATEERYKGTERERLL 1276          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|449081276|sp|O35604.2|NPC1_MOUSE (RecName: Full=NPC intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1 protein; Flags: Precursor)

HSP 1 Score: 164.081 bits (414), Expect = 9.897e-40
Identity = 153/663 (23.08%), Postives = 299/663 (45.10%), Query Frame = 0

Query:  407 ARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRF-NC----VEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMH----GILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVF-TAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLV--------DQIKQQTDIVDDVESWTD---PFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFF--------------FSPRGGKYREQFEFKKPL----------QCGQEASQLMLSKINFV--------------HKVFSGPKEHIPAMNR-------IKELIAQVNASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVG--GFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRF-MVEHGTRNQRVRQTLRNIGPAVLNG-GFSTFLAFILLADSKSHVFSSF-FKIFFLVVTFGLFHGLVFLPVILSILGPGPNAVE 998
            A RS  D        DV  + I Y ++F ++ + LG   +C    V+ +  L  AGI+ V   +  + G+ S +G+   P+      ++PFL+L +G+D++F++VQ +    E  +   L  + G  +      + ++S ++  AF  G  + +PA+ +F L+A + ++  +  Q T FV+ + +D +R E        C   ++    + + + YL   F       L K  ++ +V+ + + +   ++     ++   D +  +P D+Y+  +F    +Y   G    + +     Y +      N+V        D + QQ     +++++T      +++++ YFD   P     + Y  +   QF                +P G +  +  EF K L          +CG+       S +N V              H +     ++  AM +       I E +    +  R+FP S  Y  +E    I ++   NL +++  +F+ TL ++   + S  V++C+ +++I V   G M  W ++++ VS  NL+++ G+ V++ +HI   F M   G+R  R  + L ++G +V +G   + F   ++LA +KS +F  F F+++  +V  G  HGL+FLPV+LS +GP  N  +
Sbjct:  605 AERSIEDELNRESNSDVFTVIISYVVMFLYISLALGHIQSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYMGM---PLTLIVIEVIPFLVLAVGVDNIFILVQTYQR-DERLQEETLDQQLGRILGEVAPTMFLSSFSETSAFFFGALSSMPAVHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNHLDILCCVRGADDGQGSHASESYLFRFFKNYFAPLLLKDWLRPIVVAVFVGVLSFSVAVVNKVDIGLDQSLSMPNDSYVIDYFKSLAQYLHSGPP--VYFVLEEGYNYSSRKGQNMVCGGMGCDNDSLVQQIFNAAELDTYTRVGFAPSSWIDDYFDWVSPQSSCCRLY--NVTHQFCNASVMDPTCVRCRPLTPEGKQRPQGKEFMKFLPMFLSDNPNPKCGKGGHAAYGSAVNIVGDDTYIGATYFMTYHTILKTSADYTDAMKKARLIASNITETMRSKGSDYRVFPYSVFYVFYEQYLTIIDDTIFNLSVSLGSIFLVTLVVLGCELWS-AVIMCITIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTMSTKGSRVSRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAK 1258          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|1100165154|sp|Q9VRC9.2|NPC1B_DROME (RecName: Full=NPC intracellular cholesterol transporter 1 homolog 1b; AltName: Full=Niemann-Pick type protein homolog 1b; Flags: Precursor)

HSP 1 Score: 156.762 bits (395), Expect = 1.497e-37
Identity = 158/660 (23.94%), Postives = 279/660 (42.27%), Query Frame = 0

Query:  399 DGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGV--GMGIITAYGVCSALGLFFGPMH--------GILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRR-IEAKRDGCCPCWTHSNWTPN-----ALSQKDYLGSVFTAIGHG----LTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLV------------------DQIKQQTDIVDDVESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRG-------------GKYREQFEFKKP-LQCGQEASQLMLSKINFV------------------------HKVFSGPKEHIPAMNRIKELIAQVNASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITS-FLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNG-GFSTFLAFILLADSKSHVFSSF-FKIFFLVVTFGLFHG 979
            D L     A RS  D       G+V  + I Y ++F +V I LG       R FL  + IM    G+ I+ A  VCS LG F+G +          ++PFL+L +G+D++F++V  +  L +  K     +  G  +   G +I  T+ +++  F IG  + +PA+K+F +YA++ I+  +  Q T FVA MAID++R ++ + D  C C        N      + +   +G + T   +     L   P+K+ V+++   IT +++     +E+  D    +P ++++ K+F    +    G     +    ++Y    +   NL+                   Q  + T +     SW D + +++       + V        NSK        RG              KY   F F  P  +C +         + +                          + +S  +E       I  +  + N    IF     Y  +E    I  +   +LG++++ +F+ TL +    ITS F+VL  VI  LI++ G M  W++ ++ +S  NL++ +G+ V++ AHI   F    GT  +R R +L   G +VL+G   + F   ++L  S S +F  F F+++  +V  G  HG
Sbjct:  569 DRLDIAYMAERSIQDAIVELSEGEVSTVVISYVVMFVYVAIALGHIRSC--RGFLRESRIMLAIGGIVIVLASVVCS-LG-FWGYLDVTTTMLAIEVIPFLVLAVGVDNIFIMVHTYQRL-DHSKFKTTHEAIGEAIGQVGPSILQTAGSEMACFAIGCISDMPAVKTFAMYAAIAILLDFLLQITAFVALMAIDEKRYLDGRLDMLC-CVKSGGKKINDEDGDGVDRPKEVGLLETLFKNFYSPFLLSKPVKVSVLLIFTVITCLSLMVTPSIEKGLDQEMSMPKNSHVVKYFRYMVDLLAMGAPVYWVLKPGLNYSEPLQQ--NLICGGVECNNNSLSVQLYTQAQYPEITSLARPASSWLDDYIDWLAISDCCKYNVTTGGFCSSNSKSEDCLPCERGFTENGLRPDAETFNKYIPYFLFDLPDAECAKAGRASYADAVIYTIDDVGMSTVQDSYFMQYSTTSTTSEEFYSQLREVRRISGEINAMFKENNVDAEIFAYCVFYIYYEQYLTIWGDAMFSLGMSLVAIFLVTLLITGLDITSTFIVLFMVICILINMLGMMWAWSINLNAISLVNLVVCVGIGVEFVAHIVRSFKRAEGTAQERARHSLNVTGSSVLSGITLTKFAGIVVLGFSNSQIFQVFYFRMYLGIVLIGAAHG 1220          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|73921247|sp|Q6T3U3.1|NPCL1_RAT (RecName: Full=NPC1-like intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1-like protein 1; Flags: Precursor)

HSP 1 Score: 144.436 bits (363), Expect = 1.124e-33
Identity = 161/686 (23.47%), Postives = 280/686 (40.82%), Query Frame = 0

Query:  399 DGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFN-----CVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFG-PMHGILPFLLLGIGIDDMFVIVQCFNTLTE---EEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWG----NVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIY--------------------CHNVDYIHDYESIANLVDQ---IKQQTDIVDDVESWTDP---------------------------FTNYMNKYFDPDFPVLPYNQSYFNSKLSQ--FFFSPRGG--KYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGR----------IFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLIS-NVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVE-HGTRNQRVRQTLRNIGPAVLNG-GFSTFLAFILLADSKSHVFSS-FFKIFFLVVTFGLFHGLVFLPVILSILGPGPNAVEIELDE 1003
            D  +   +A RS  D    T + D+    + Y IVF ++ + LG ++      VE +A L   G++ V   ++ A G  S LG+     +  ++PFL+L +G D++F+ V  +  L     E++        G T+     ++ + S+++ I F +G  T +PA+++F L + + II  +  Q T FVA +++D +R EA R     C++     P    +   L          L    I+ +V++L L + G  ++     NV L+QE      LP D+Y+  +F+    Y   G     +                     C +       +  +   DQ       +  VDD   W  P                             N MN+   P  P       Y    L+       P+GG   YR                Q++ S+    HK     ++   A+   + L A + A  R          +FP +     ++    +  E    L L  +  F+    L+  ++ +  L L+ +I+ L+D  G M  W ++ + VS  NL+ A+G+ V++ +HI   F V    TR +R +     +G AV  G   + F   ++L  +++ +    FF++  L+   GL HGLVFLPV+LS LGP  N   ++ ++
Sbjct:  609 DKFQVAFSAERSLEDEINRTTIQDLPVFAVSYIIVFLYISLALGSYSRCSRVAVESKATLGLGGVIVVLGAVLAAMGFYSYLGVPSSLVIIQVVPFLVLAVGADNIFIFVLEYQRLPRMPGEQRE----AHIGRTLGSVAPSMLLCSLSEAICFFLGALTPMPAVRTFALTSGLAIILDFLLQMTAFVALLSLDSKRQEASRPDVLCCFSTRKLPPPKEKEGLLLRFFRKIYAPFLLHRFIRPVVMLLFLTLFGANLYLMCNINVGLDQELA----LPKDSYLIDYFLFLNRYLEVGPPVYFVTTSGFNFSSEAGMNATCSSAGCKSFSLTQKIQYASEFPDQSYVAIAASSWVDDFIDWLTPSSSCCRLYIRGPHKDEFCPSTDTSFNCLKNCMNRTLGPVRPTAEQFHKYLPWFLNDPPNIRCPKGGLAAYRTSVNLS-------SDGQVIASQFMAYHKPLRNSQDFTEALRASRLLAANITADLRKVPGTDPNFEVFPYTISNVFYQQYLTVLPEGIFTLALCFVPTFVVCYLLLGLDMCSGILNLLSIIMILVDTIGLMAVWGISYNAVSLINLVTAVGMSVEFVSHITRSFAVSTKPTRLERAKDATVFMGSAVFAGVAMTNFPGILILGFAQAQLIQIFFFRLNLLITLLGLLHGLVFLPVVLSYLGPDVNQALVQEEK 1279          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|62906873|sp|Q03602.2|PTR9_CAEEL (RecName: Full=Patched-related protein 9)

HSP 1 Score: 137.117 bits (344), Expect = 1.120e-31
Identity = 128/571 (22.42%), Postives = 251/571 (43.96%), Query Frame = 0

Query:  432 IVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQK----DYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFP-FGG-DRVIIYCHNVDYIHDYESIANLVDQIKQQ--TDIVDDVESWTDPF--TNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGP 992
            ++  F M      N V+ + F+ F  +    M  I +  +   L   F P+  I+PFL++ IG D+MF++++ +      +KS     ++   +  +  ++ +TS+TD ++F IG  +   A++ FC Y ++ I+  + +Q TFF A M++  RR  + +     C+  +      + +K    D+   V   +   L   P +I V  + LA   ++I   + L    D+    P D+Y++K     +  F  +GG    ++   N+  + D     NL+   +    ++     E W DPF   N   KY + DF          + +L  F       K+R    F   +    EA ++M      V K+  G     P    +++++     +G ++  S  + L +        +  ++  A+L +    + ++   +++  +   ++   I V G +   N  +D +S   +++++G  VDY  H    F+++   R+ R+ + L  +   +L    ST +   LL+   S++  +F    F VV  G+ HGL+FLPV+L  + P
Sbjct:  274 VLVVFCMFACSSLNIVKSQPFIGFFAMFNATMATIASTSLLLYLQYPFLPLVFIMPFLVVSIGTDNMFLMLKSWRM---TKKSSNEEQRYIHALTESAASLFLTSLTDGLSFAIGSISDFHAVRVFCTYCAMAILFMFLFQVTFFNAVMSLCCRREVSGKHPVFCCYEITPPEETQVLEKQSNFDFSSIVAKHLFKILNPLPSRIGVFFIFLAYLFISIHFAIGLPLGLDLKLLAPDDSYVSKELEAQERLFADYGGFCFALVRAENIS-LKDPMVRRNLIGLYRDLGGSEYSSPSEFWLDPFEKKNRGKKYSESDF----------SDELHTFLAKEPHLKFRNDIRFT--MMGKIEAMKMMFR----VRKL--GKDNDAPRAEYMRKVMENSQFNGFVYDTS--FLLVDQQMTTVYNVIVDVVSAILTMLSICVLMVPRPVSAMCIAFAILSVNIGVIGALAATNTRLDIISMITIVMSVGFSVDYVTHTTFHFVIQ---RDNRLEKCLLVMTEPILQSALSTAIGVSLLSFVHSYIVRTFVNTVFFVVGLGILHGLIFLPVLLDTIVP 817          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|74676548|sp|Q12200.1|NPC1_YEAST (RecName: Full=NPC intracellular cholesterol transporter 1-related protein 1; AltName: Full=Niemann-Pick type C-related protein 1; Flags: Precursor)

HSP 1 Score: 127.872 bits (320), Expect = 1.493e-28
Identity = 145/643 (22.55%), Postives = 283/643 (44.01%), Query Frame = 0

Query:  386 EMLKIMLNETGYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHG-ILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIH--------------DYESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKYFD--------------PDFPV----LPYNQSYFNSKLSQFFFSPRGGKYREQFEF-----KKPLQCGQEA---SQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGRI---------FPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMV----EHGTRNQRVRQTLRNIGPAVLNG-GFSTFLAFILLADSKSHVFSSF-FKIFFLVV 972
            E L+  L +   P+GL+   N   S      +    D+  + I Y ++F +    L R +  + R  L  +G++ V   I+ A G  +  GL    +   ++PFL+L IGID++F+I   ++   E++    +  K  + +     +I ++ +     F I     +PA+ +F +Y++V +I     Q T +V+ +++ ++R   K+            T N  +++ +L + +  +   LT+   K L+II+  A    ++     ++   D T  +P D+Y+  +F K+   F   G  V +   N+D                 + +S+AN+++Q + ++ I + + +W D +  ++N   D              P FP       + Q  +N  +S F   P G  + E           P   G  A   + L+ ++ +    VF     H P  ++ K+ I   +   RI         F  S  Y  +   + +     + +G A++ +F  +   + N+ +SFL+ + V + ++D+G  M    ++++ VS  NLII +GL V++  HI   F V         N RV  +L  IG +V+ G   + F+   +LA ++S +F  F F+++F ++
Sbjct:  523 ERLEEYLLDLKVPEGLRISFNTEISLEKELNNN--NDISTVAISYLMMFLYATWALRRKD-GKTRLLLGISGLLIVLASIVCAAGFLTLFGLKSTLIIAEVIPFLILAIGIDNIFLITHEYDRNCEQKPEYSIDQKIISAIGRMSPSILMSLLCQTGCFLIAAFVTMPAVHNFAIYSTVSVIFNGVLQLTAYVSILSLYEKRSNYKQ-----------ITGNEETKESFLKTFYFKM---LTQ---KRLIIIIFSAWFFTSLVFLPEIQFGLDQTLAVPQDSYLVDYF-KDVYSFLNVGPPVYMVVKNLDLTKRQNQQKICGKFTTCERDSLANVLEQERHRSTITEPLANWLDDYFMFLNPQNDQCCRLKKGTDEVCPPSFPSRRCETCFQQGSWNYNMSGF---PEGKDFMEYLSIWINAPSDPCPLGGRAPYSTALVYNETSVSASVFR--TAHHPLRSQ-KDFIQAYSDGVRISSSFPELDMFAYSPFYIFFVQYQTLGPLTLKLIGSAIILIFFISSVFLQNIRSSFLLALVVTMIIVDIGALMALLGISLNAVSLVNLIICVGLGVEFCVHIVRSFTVVPSETKKDANSRVLYSLNTIGESVIKGITLTKFIGVCVLAFAQSKIFDVFYFRMWFTLI 1138          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|73921246|sp|Q6T3U4.1|NPCL1_MOUSE (RecName: Full=NPC1-like intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1-like protein 1; Flags: Precursor)

HSP 1 Score: 125.561 bits (314), Expect = 7.132e-28
Identity = 164/717 (22.87%), Postives = 286/717 (39.89%), Query Frame = 0

Query:  367 NSVKERNEPITHGTLEFEGEMLKIMLNETGYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVG-----------MGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTE---EEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKD-----YLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFG-----------------GDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPF----------------------------------TNYMNKYFDPDFPVLPYNQSYFNSKLSQF--FFSPRGG--KYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGR----------IFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLIS-NVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVE-HGTRNQRVRQTLRNIGPAVLNG-GFSTFLAFILLADSKSHVFSS-FFKIFFLVVTFGLFHGLVFLPVILSILGPGPN 995
            N+    +  + H  L  E  + ++   +    D  +   +A RS  D    T + D+    I Y IVF ++ + LG ++   + A  S A +   G           MG  +  GV S+L +       ++PFL+L +G D++F+ V  +  L     E++        G T+     ++ + S+++ I F +G  T +PA+++F L + + II  +  Q T FVA +++D +R EA R     C++  N  P    QK+     +   ++T     L    I+ +V++L L + G  ++    +    D    LP D+Y+  +F+    Y   G                 G   I      +     + I     +   Q+ +     SW D F                                   N MN+   P  P       Y    L+       P+GG   YR                Q++ S+    HK     ++   A+   + L A + A  R          +FP +     ++    +  E    L L  +  F+    L+  ++ +  L L+ +I+ L+D  G M  W ++ + VS  NL+ A+G+ V++ +HI   F V    TR +R +     +G AV  G   + F   ++L  +++ +    FF++  L+   GL HGLVFLPV+LS LGP  N
Sbjct:  577 NNYPADDPRMAHAKLWEEAFLKEMQSFQRSTADKFQIAFSAERSLEDEINRTTIQDLPVFAISYLIVFLYISLALGSYSRWSRVAVDSKATLGLGGVAVVLGAVVAAMGFYSYLGVPSSLVII-----QVVPFLVLAVGADNIFIFVLEYQRLPRMPGEQRE----AHIGRTLGSVAPSMLLCSLSEAICFFLGALTSMPAVRTFALTSGLAIIFDFLLQMTAFVALLSLDSKRQEASRPDVVCCFSSRNLPPP--KQKEGLLLCFFRKIYTPF---LLHRFIRPVVLLLFLVLFGANLYLMCNISVGLDQDLALPKDSYLIDYFLFLNRYLEVGPPVYFDTTSGYNFSTEAGMNAICSSAGCESFSLTQKI-QYASEFPNQSYVAIAASSWVDDFIDWLTPSSSCCRIYTRGPHKDEFCPSTDTSFNCLKNCMNRTLGPVRPTTEQFHKYLPWFLNDTPNIRCPKGGLAAYRTSVNLS-------SDGQIIASQFMAYHKPLRNSQDFTEALRASRLLAANITAELRKVPGTDPNFEVFPYTISNVFYQQYLTVLPEGIFTLALCFVPTFVVCYLLLGLDIRSGILNLLSIIMILVDTIGLMAVWGISYNAVSLINLVTAVGMSVEFVSHITRSFAVSTKPTRLERAKDATIFMGSAVFAGVAMTNFPGILILGFAQAQLIQIFFFRLNLLITLLGLLHGLVFLPVVLSYLGPDVN 1271          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|1042851721|sp|H2L0G5.1|PTC3_CAEEL (RecName: Full=Protein patched homolog 3)

HSP 1 Score: 120.168 bits (300), Expect = 3.639e-26
Identity = 85/320 (26.56%), Postives = 152/320 (47.50%), Query Frame = 0

Query:  726 FTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASG-RIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSK-SHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGPGPNAVEIELDEHGINVIENGRVIATELVETSVNVNSGQNGNPQDRAKSYQL 1043
            F NY+  +F+ D      N  Y+ S+ S F+ +P G +Y E+     P      A  L+ S++ F           +  +  I+    + +  G    P    +T WE    +   L++ + +  L VF     L+ N   + L++  V+++ I++GGFM    + ++ +SA  LI A+G+ V+++AH+   F+   GT +QR+   L+++   V +G  STFL  ++L  S+   V + FF    L+V  G+F+GL  LPVIL+++GP P     +    G +V+     +  +  E S  V  G     + R    ++
Sbjct: 1040 FYNYLTGWFNVD------NMMYYVSQAS-FYPTPPGWEYNEKLAKVVP-----AAEPLLYSQMPFYQNDLIDTPAIVKMIEEIRATCEEYSERGLSNHPSGIAFTFWEQYLTLRWNLFQAICIIALAVFCVISILMFNPWAATLIMCIVVITTIELGGFMGLMGIKMNPISAVTLICAVGIGVEFTAHVELAFLTALGTIDQRLESCLQHMFVPVYHGAISTFLGVVMLVFSEFDFVVTYFFYTMTLLVALGVFNGLCVLPVILTLVGPKPELTPTD----GSSVLPPPPPLRQQYAEKSGGVEGGMRKRKEKRPAEVEM 1343          

HSP 2 Score: 78.9518 bits (193), Expect = 1.470e-13
Identity = 55/199 (27.64%), Postives = 99/199 (49.75%), Query Frame = 0

Query:  408 RRSFGDVAGSTILGDVGKLG--------IGYTIVFFFVMIMLGRFN----CVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFF-GPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRR-IEAKRD 592
            RR F  +A ++I   + +          IGY ++  +     GRF      V+    L+  G++ V +  I   G  + LG+ F      ++PFL LG+GIDDMF+++  ++ +     ++   ++ G  ++  G+++ +TSI +++AF  G    IPAL+SFC   ++ +     +    F A + ID RR  + KRD
Sbjct:  605 RRQFHPLASTSIADMLEEFSQFNYIIIVIGYILMVIYAAFTQGRFQGWWLAVQSNVALAICGVILVTISSICGLGFATHLGINFNAATTQVVPFLSLGLGIDDMFLLLHNYDEII----NICNKNEIGVLLKETGMSVMLTSINNILAFISGYVLPIPALRSFCSQTAILLAFNLIFLMFIFPAMIGIDLRRQRKGKRD 799          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1325261885|ref|XP_023331667.1| (patched domain-containing protein 3-like isoform X1 [Eurytemora affinis] >gi|1325261897|ref|XP_023331674.1| patched domain-containing protein 3-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 993.416 bits (2567), Expect = 0.000e+0
Identity = 480/931 (51.56%), Postives = 631/931 (67.78%), Query Frame = 0

Query:   62 LDAMKNGIISGMERFFFFYGKAVANRPGWFIFLCILIVSASGSGLMKFYSENVGHKLWISEASSSRKNVDWLWENYPPSFRFASLIFEAEDILVPEVIQSMYSIHKEIEAIKTVHGEGWRDNCQMVPVFKAPDISQILSFGRRKRDTQNITEPADGFETSFQDDFFSEDDGFGNVGLDDIPANTTDETNGMLDMAEAFSADSYPQPYCDIVEGIETACFEMSILELWANDGSYDDQTDSDIASLTKRSIIDKLNQVNTSGVFLIQRNFSSFLSEITYDPTNGQIIAARATVMRWFGRMNGTEALHNSVKERNEPITHGTLEFEGEMLKIMLNETGYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGP 992
            L+ ++   +  +E  F  YG+ VA  P   I  C+ +    G GL  F +EN G KLWI   S  R N DW++ENYP   RF SLI   E++L  +V+++MY + K I  ++ ++ E W D+C   PV K P +  I  FGR++R            E  F DD F           D+            L +A  FS D YP PYC IV G+  AC E+SILEL+   G YD++TD  + +LT   ++D +N  N SG+FL   NF+S+LS I Y+ T G+II A+AT + W GRMN TEA  N    R EPI+   L++EG+ML++MLN++ YP GL+++ N  RSFGD+AGSTI+GD+     GY ++F +V IMLG+F+CVE R +LS  GI+GV MGII +YG+CSA G F+GPMH ++PFL+LGIGIDDMFVI+QC++TL+EEEK   L+ +FG  M+ AG AIT+TS TDVIAFG+G  T++PAL+SFC+YA+VGI+AT+ +Q +FF+AWM +DQRRIE+ R+GCCPC+ H         +K  L  VF      L K P+KI V+++ L +TG  ++GNVLL QEFD TWFLP DTYIA+WFV NK YFP  G+R  IY    +   D + I  L   +++QTDI+   ++WT P+ +Y+ K    + P    N++ F S LSQF FSP G  YR  F FK+ L+CG+ AS++ LS+I + H++ +GP+EH+PAMNR+K +I   N SG++F M+ GY  WETDEIIS E+YRN+ LA++CVFITTL  ISN+  S L+LVCV L+L+DVGGFMHFW LTIDTVS NNLIIAIGLCVDYSAH+ HRF+ E G R  RV QT+ NIGPAV NGG STFLAF+LLA S+SHVFSSFFKIFFLVV+FGLFHGLV LPVILS +GP
Sbjct:   17 LNKLQGSTLRILEDVFQSYGEFVARHPVIVILSCVALTCVCGGGLYWFRAENEGVKLWIPYNSDYRLNNDWMFENYPRDLRFNSLILTGENVLTAQVLRTMYRVSKSIRTMRNINNETWDDHCMKRPVIKVPSLFDI--FGRKRR------------EIKFDDDLFG----------DEFDEEKDTVITDPLKLATQFSIDEYPDPYCGIVTGMPLACMELSILELFGQGGDYDERTDDVMETLTDEIVLDVINNKNVSGIFLTPYNFTSYLSNIEYN-TQGEIIGAKATYIIWLGRMNMTEAKLNPAPGRGEPISMSMLQWEGDMLEVMLNKSDYPKGLESFPNVARSFGDIAGSTIIGDITFFVAGYIMMFTYVQIMLGKFSCVEHRTYLSTVGILGVIMGIIVSYGICSAAGHFYGPMHSVMPFLMLGIGIDDMFVIIQCWDTLSEEEKKENLVKRFGFLMKQAGAAITVTSATDVIAFGVGAFTILPALQSFCIYAAVGIVATFVFQSSFFLAWMNLDQRRIESNRNGCCPCYVHPETNNQTQERKGALQRVFKKYAEYLIKTPVKISVLLVALVLTGFGVYGNVLLRQEFDPTWFLPQDTYIAQWFVNNKMYFPSSGERGTIYFSGTELPADIDKIEKLSQDLREQTDIIVKSDAWTRPYIDYLQKLNYTEKPS-KLNKTAFRSSLSQFLFSPVGAVYRNGFNFKEQLECGELASEVQLSEITYTHRLMNGPEEHVPAMNRVKNIIESANISGKVFAMAMGYATWETDEIISLEIYRNMALAIVCVFITTLLFISNLGGSLLILVCVCLTLVDVGGFMHFWGLTIDTVSCNNLIIAIGLCVDYSAHVMHRFLTEQGPREDRVVQTVSNIGPAVFNGGVSTFLAFVLLATSRSHVFSSFFKIFFLVVSFGLFHGLVVLPVILSFIGP 921          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1325261904|ref|XP_023331682.1| (patched domain-containing protein 3-like isoform X3 [Eurytemora affinis])

HSP 1 Score: 993.03 bits (2566), Expect = 0.000e+0
Identity = 480/931 (51.56%), Postives = 631/931 (67.78%), Query Frame = 0

Query:   62 LDAMKNGIISGMERFFFFYGKAVANRPGWFIFLCILIVSASGSGLMKFYSENVGHKLWISEASSSRKNVDWLWENYPPSFRFASLIFEAEDILVPEVIQSMYSIHKEIEAIKTVHGEGWRDNCQMVPVFKAPDISQILSFGRRKRDTQNITEPADGFETSFQDDFFSEDDGFGNVGLDDIPANTTDETNGMLDMAEAFSADSYPQPYCDIVEGIETACFEMSILELWANDGSYDDQTDSDIASLTKRSIIDKLNQVNTSGVFLIQRNFSSFLSEITYDPTNGQIIAARATVMRWFGRMNGTEALHNSVKERNEPITHGTLEFEGEMLKIMLNETGYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGP 992
            L+ ++   +  +E  F  YG+ VA  P   I  C+ +    G GL  F +EN G KLWI   S  R N DW++ENYP   RF SLI   E++L  +V+++MY + K I  ++ ++ E W D+C   PV K P +  I  FGR++R            E  F DD F           D+            L +A  FS D YP PYC IV G+  AC E+SILEL+   G YD++TD  + +LT   ++D +N  N SG+FL   NF+S+LS I Y+ T G+II A+AT + W GRMN TEA  N    R EPI+   L++EG+ML++MLN++ YP GL+++ N  RSFGD+AGSTI+GD+     GY ++F +V IMLG+F+CVE R +LS  GI+GV MGII +YG+CSA G F+GPMH ++PFL+LGIGIDDMFVI+QC++TL+EEEK   L+ +FG  M+ AG AIT+TS TDVIAFG+G  T++PAL+SFC+YA+VGI+AT+ +Q +FF+AWM +DQRRIE+ R+GCCPC+ H         +K  L  VF      L K P+KI V+++ L +TG  ++GNVLL QEFD TWFLP DTYIA+WFV NK YFP  G+R  IY    +   D + I  L   +++QTDI+   ++WT P+ +Y+ K    + P    N++ F S LSQF FSP G  YR  F FK+ L+CG+ AS++ LS+I + H++ +GP+EH+PAMNR+K +I   N SG++F M+ GY  WETDEIIS E+YRN+ LA++CVFITTL  ISN+  S L+LVCV L+L+DVGGFMHFW LTIDTVS NNLIIAIGLCVDYSAH+ HRF+ E G R  RV QT+ NIGPAV NGG STFLAF+LLA S+SHVFSSFFKIFFLVV+FGLFHGLV LPVILS +GP
Sbjct:   17 LNKLQGSTLRILEDVFQSYGEFVARHPVIVILSCVALTCVCGGGLYWFRAENEGVKLWIPYNSDYRLNNDWMFENYPRDLRFNSLILTGENVLTAQVLRTMYRVSKSIRTMRNINNETWDDHCMKRPVIKVPSLFDI--FGRKRR------------EIKFDDDLFG----------DEFDEEKDTVITDPLKLATQFSIDEYPDPYCGIVTGMPLACMELSILELFGQGGDYDERTDDVMETLTDEIVLDVINNKNVSGIFLTPYNFTSYLSNIEYN-TQGEIIGAKATYIIWLGRMNMTEAKLNPAPGRGEPISMSMLQWEGDMLEVMLNKSDYPKGLESFPNVARSFGDIAGSTIIGDITFFVAGYIMMFTYVQIMLGKFSCVEHRTYLSTVGILGVIMGIIVSYGICSAAGHFYGPMHSVMPFLMLGIGIDDMFVIIQCWDTLSEEEKKENLVKRFGFLMKQAGAAITVTSATDVIAFGVGAFTILPALQSFCIYAAVGIVATFVFQSSFFLAWMNLDQRRIESNRNGCCPCYVHPETNNQTQERKGALQRVFKKYAEYLIKTPVKISVLLVALVLTGFGVYGNVLLRQEFDPTWFLPQDTYIAQWFVNNKMYFPSSGERGTIYFSGTELPADIDKIEKLSQDLREQTDIIVKSDAWTRPYIDYLQKLNYTEKPS-KLNKTAFRSSLSQFLFSPVGAVYRNGFNFKEQLECGELASEVQLSEITYTHRLMNGPEEHVPAMNRVKNIIESANISGKVFAMAMGYATWETDEIISLEIYRNMALAIVCVFITTLLFISNLGGSLLILVCVCLTLVDVGGFMHFWGLTIDTVSCNNLIIAIGLCVDYSAHVMHRFLTEQGPREDRVVQTVSNIGPAVFNGGVSTFLAFVLLATSRSHVFSSFFKIFFLVVSFGLFHGLVVLPVILSFIGP 921          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1061485453|gb|ODN02586.1| (Patched domain-containing protein 3, partial [Orchesella cincta])

HSP 1 Score: 797.349 bits (2058), Expect = 0.000e+0
Identity = 422/947 (44.56%), Postives = 593/947 (62.62%), Query Frame = 0

Query:   69 IISGMERFFFFYGKAVANRPGWFIFLCILIVSASGSGLMKFYSENVGHKLWISEASSSRKNVDWLWENYPPSFRFASLIFEAEDILVPEVIQSMYSIHKEIEAIKTVHGEGWRDNCQMVPVFKAPDISQILSFGRRKRDTQNITEPADGFETSFQDDFFSEDDGFGNVGLDDIPANTTDETNGMLDMAEAFSADSYPQPYCDIVEGIETACFEMSILELWANDGSYDDQTDSDIASLTKRSIIDKLNQVNTSGVF--LIQR--NFSSFLSEITYDPTNGQIIAARATVMRWFGRMNGTEALHNSV---KERNEPITHGTLEFEGEMLKIMLNETGYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVFTAI-GHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVN---ASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGPGPNAVEIELDEH 1004
            I+  +ERFF+ +G  +   P  FI  C+++    G GL++F  EN   KLWI   S   K+ +WL +N+P  FRF   I  AED+L P+V+  +  IH +++ I+      W+D C  +PV K  D ++     RRKR+  N    A  F  +F     + D         + P           D + +   D Y    C+IV  ++  CFE+++LELW       +   S I  LTK  I++ +N      VF  +  R  N+++ L ++  + + G II A A ++RWF R+N T      V   +   + +      +E E+ K  L+    P+G+  Y N  RSF D++GSTI+GD   L +G++IVF +V+ MLG+FN VEQR  LS AG+  VG+ I+ +YG+CSA  + +GP+H ILPFLLLGIGIDDMFVI+QC+N L  E+    + +K G T+QHAGV+ITITS+TD  AF IGG+TV+PAL+SFC+YA++GI+ATY +Q TFFVAW+AIDQ+RIE +R+   P   H++++P  L  + +  +    I    L +   K++V ++T  + GV +WGN+LL QEF+  WFLP D+Y+ K+F    ++FP  G+   I  ++VD   +   + +L+   +  T  V ++++W  PF  Y+N  F  D P        FN  L +F FSP GGKY+ +F+F K L CG+ A  +MLS  NF   +FSGP++H+PAMN IK +I + N   A+   F  S  +  WETDEII++ELYRN+GLA++CVF+TTL LI+N+ T  +VL+CVIL+L+DVGG MHFWNLTIDTVS  +L++AIGLCVDY+AHIAH FM   GTRN+R  + L NIGPAVLNGGFSTFLAFILLA+SKSHVF +FFKIFFLVV +GLFHGLVFLPV+LSI+GP P A  +  DE+
Sbjct:   63 IVGSLERFFYRWGYQIGLTPVPFIVACVILTVLCGIGLLRFTPENRPDKLWIPWNSEFVKDTEWLNKNFPSPFRFHYAIITAEDVLQPKVLDVLLEIHNKVKEIEA-STYKWKDICFKLPVIKF-DTNK-----RRKREVNNANNSATLFNGNFDGFDSAFDFDDDEEATSEKP----------FDPSVSLPTDVY----CNIVSSLDKECFELNMLELW-------NYNSSVINKLTKEEIVNAINHEQIRQVFYPVFGRPVNYATELGKLQRNES-GYIIGAEAVMIRWFARLNRTAMNLGDVLNDEGTGQAVDKYNFMWEKELSKFFLSFRP-PEGVNIYFNVARSFADISGSTIMGDASMLAVGFSIVFLYVIFMLGKFNFVEQRPLLSLAGLSCVGLAILVSYGICSAFKIIYGPVHNILPFLLLGIGIDDMFVIMQCWNNLRPEDMKKSIPEKLGLTLQHAGVSITITSLTDFFAFAIGGTTVLPALQSFCVYAALGIVATYIFQATFFVAWLAIDQKRIERRRNAFIPYIIHASFSPKTLMTRSFAQTAIEKIYCKVLFRKFSKVMVFLVTFGVFGVNVWGNILLRQEFNPIWFLPQDSYLFKYFAARDQFFPEKGEEGFILMNDVDLNSNLPKLESLLLDFENST-FVHEIDAWYPPFKQYVNSNFGKDIPNQKLTNKEFNKYLGKFLFSPTGGKYQSRFKFDKNLTCGKSAPAVMLSSANFKFNIFSGPEQHVPAMNNIKRIIREANFTPANSHTFIWSFAFAGWETDEIITDELYRNMGLAMICVFLTTLVLIANLQTCCMVLLCVILTLVDVGGSMHFWNLTIDTVSCIDLVLAIGLCVDYAAHIAHAFMTSVGTRNERSTKALVNIGPAVLNGGFSTFLAFILLANSKSHVFLTFFKIFFLVVVYGLFHGLVFLPVLLSIIGPAPYATAVLEDEN 978          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1022768415|gb|KZS13150.1| (Niemann-Pick C1 protein [Daphnia magna])

HSP 1 Score: 747.273 bits (1928), Expect = 0.000e+0
Identity = 409/958 (42.69%), Postives = 596/958 (62.21%), Query Frame = 0

Query:   52 KSGRNPCLKCLDAMKNGIISGMERFFFFYGKAVANRPGWFIFLCILIVSASGSGLMKFYSENVGHKLWISEASSSRKNVDWLWENYPPSFRFASLIFEAEDILVPEVIQSMYSIHKEIEAIKTVHGEGWRDNCQMVPVFKAPDISQILSFGRRKRDTQNITEPADGFETSFQDDFFSEDDGFGNVGLDDIPANTTD-ETNGMLDMAEAFSADSYPQPYCDIVEGIETACFEMSILELWANDGSYDDQTDSDIASLTKRSIIDKLNQVNTSGVFLIQRNFSSFLSEITYDPTNGQIIAARATVMRWFGRMNGTEALHNSVKERN----EPITHGTLEFEGEMLKIMLNETGYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVFTA-IGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQE-ASQLMLSKINFVHKVFSGPKEHIPAMNRIKELI--------AQVNASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGPGP 994
            +S       C++      ++  E FF  +G  VA  P  +I   ++I +    GL+ F +EN   KLWI + S   +N +WL EN+P   R  S +   +++L PEV+  M  +H  I A+ +  G  WRD C     FK P +++  +  RRKR   NI+E  D F+  F+D  F   DGF +   +++P+++ D +    +D+            +CDIVEG+E AC E +IL++W    SY  +    I+ L++  II+ +N V  S +  +Q  +SS L +  ++ T G I++A + +++ F R+N +  + N V + +    E +    LE+E   + ++ N T  P+  K Y  A RSFGD++ +TILGDV  L  G  +VF +V +MLG+FN VEQRAFLS  GI  V M I+ +YG+CSA G+F+GP+H ILPFLLLGIGIDDMFVIVQC++ L +EE    L  + G TM+HAGV+ITITS+TD  AF +G +TV+P+L+SFC+Y++VGI+ATY +Q +FFVAW+ +DQ+RIE+ RDG  PC+ H NW P++LS+ + L   F   +   L K P K+ +++L   + G+  WG+VL+ QEF+  WF+P  TY++++F   + +FP  G    IY    +   + + +  L+D ++ +T+IV  V+ W   F  +  K    D+         F++ L  + F+ +G K+R  F+F+ PLQC    A  + ++  +FV + FSG +EHIPAM RIK++         +QV AS RIF      + WETDE+I EELYRNL +A++CVFITT  LI+N+    LVL+CV+L+L+ V G MHFW LTIDTVS  NL++AIGLCVDY+AH+AH FM + GTRN+R   T+ +IGPAV +GGFSTFLAFI LA+S SHVF +FFKIF LVV +GLFHGL+F PVILS++GP P
Sbjct:   13 QSTSTSTKSCVNRASFIFMANFENFFLRWGITVAKNPWTYIIASVVIAATCSIGLLNFTAENRPTKLWIPQDSGFVQNSEWLQENFPNEIRLQSWVVVDDNVLRPEVLLWMLELHDNITAVSSASGYQWRDVC-----FKVPVVTEQQTNQRRKRQL-NISE-NDPFD--FEDFNF---DGF-DANFEEMPSSSIDPDFEPAIDLPTGI--------FCDIVEGLEEACLENNILDIW----SYKKEV---ISKLSREDIINTINTVQKSNLTRVQ--YSSLLGQPRFNET-GHIVSAGSLLVQLFSRVNYS-LIANDVTDNDAGTGEVVDPINLEWEKAFVNLLHNFTSPPNSSKLYYMAARSFGDISSATILGDVRLLAAGICVVFIYVQLMLGKFNLVEQRAFLSLTGIASVAMAIVVSYGLCSAFGVFYGPVHSILPFLLLGIGIDDMFVIVQCWSNLNKEEMKRSLPVRIGITMKHAGVSITITSVTDFAAFAVGATTVLPSLRSFCIYSAVGILATYIFQASFFVAWLTLDQKRIESHRDGMIPCFVHRNWKPSSLSRIEPLQLFFNRFLAKYLFKWPAKVFILVLAAFLFGINTWGSVLMRQEFNPLWFIPTSTYLSQYFSVIESHFPDNGQMASIYIQTTNLSSNLDRLDALIDTVRNETNIVSQVDDWFGGFKEFTAKRHAIDWMNQNMTDEQFSTYLKNYLFTQKGAKFRRNFKFEDPLQCRSAIAPPISITNFDFVFRPFSGREEHIPAMRRIKDIAEDFSQQFGSQVFASARIF------SSWETDEVIMEELYRNLAIAMVCVFITTFILIANLFACVLVLLCVVLTLVCVNGTMHFWGLTIDTVSCINLVLAIGLCVDYAAHVAHTFMTKTGTRNERAAATVSSIGPAVFHGGFSTFLAFIFLANSDSHVFITFFKIFVLVVAYGLFHGLLFFPVILSLIGPPP 932          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1325286696|ref|XP_023328081.1| (Niemann-Pick C1 protein-like [Eurytemora affinis])

HSP 1 Score: 740.339 bits (1910), Expect = 0.000e+0
Identity = 403/968 (41.63%), Postives = 594/968 (61.36%), Query Frame = 0

Query:   69 IISGMERFFFFYGKAVANRPGWFIFLCILIVSASGSGLMKFYSENVGHKLWISEASSSRKNVDWLWENYPPSFRFASLIFEAEDILVPEVIQSMYSIHKEIEAIKTVHGEGWRDNCQMVPVFKAPDISQILS-----FGRRKRDTQNITEPA----DGFETSFQDDFFSEDDGFGNVGLDDIPANTTDETNGMLDMAEAF--SADSYPQPYCDIVEGIETA-CFEMSILELWANDGSYDDQTDSDIASLTKRSIIDKLNQVNTSGVFLIQRNFSSFLSEITYDPTNGQIIAARATVMRWFGRMNGTEALHNS---VKERNEPITHGTLEFEGEMLKIMLNETGYPDGLKTYA-NARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSL--PLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPFTNYM---NKYFDPD-FPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGPGPNAVEIELDEHGINVIENGRV 1014
            I+  ME FF++YG  +A  P   I L ++I      GL++   EN  +KLWI + S   +N +WLW+N+P   RF S+I   +++L PE I+ +Y +H+ +  + +  GE W D C  VP+     I  +L+     +   + D + I E      DG   +  D  F        +  D+    +++ +   +  A  F  + D YP+ YC ++E + T+ C E S+LELW   G Y  +T   I +LT+  I+  +N V TS V+   ++FS +L +I+ + ++G II ARA  +R+FG++N T AL +S      +  P+   TL +E  ++ ++   +   DG  +   N  +SF D++   I GD    GIG + VF +V IMLG+FN V+ R  LS  G+   G+ I+T+YG CS +GL F P+H ++PFL++G+G+DDMFVI+Q  + L  +  +    +  K   TM+  GVAITITS TD+IAF IG  TV+PALKSFCL+ +VGI + YFY  T FVA+++ID+RRI AKR+G CP   H +WTPN +SQ+++   +F  +G  L+   ++  V+I T  + G  I+G   L+QEF+   F+PP++Y+A WF  N EYFP  G+RVI+   ++DY ++   + NLV  +  +T+I+  V+SW   F  +    N   + D F +   N+  F + L+QF FSP G  YR +F F   L CG+ A  ++LS I   H++F+ P E IPAMNRIKEL+ + N S R FP    Y  WETDE+I  ELY+N+G+++LCVF+T L LI+++    LVLVCV+LSL++V G+MHFW LT+D +S  NL IAIGL VDYSAH+AH F+++ G++++R+R TL  IGPAVLNGGFSTFLAFI    SKSHVF SFF+IF LVV FGL+HGL+FLPV L+++GP P+    E D+  +N  E G++
Sbjct:   31 IVHSMELFFYWYGSILARYPIRSICLTLVIPGLCAIGLLRLEQENNPYKLWIPQDSDYIRNHEWLWQNFPDDIRFHSVILTGDNVLTPEYIRKLYEVHEAMVNVTSSKGERWSDICYRVPMVDLEPIQDLLNDSLYEYEDYEDDVEEIPETTEQIQDGGSETTADSMFDITTQISVI--DNSTLISSNNSENRIKRASEFDPAIDLYPEDYCPLIEDLTTSICLENSLLELWGKAG-YTQETKDIIYALTQEDILRAINTVTTSPVYDRAQDFSKYLGDISRN-SSGHIIGARAMFLRFFGQVN-TTALTDSEDVAVTKGSPVDGNTLAWEDAIIDVL---SKLADGDSSLVFNVAKSFSDLSAGAIYGDALLFGIGTSTVFVYVQIMLGKFNFVQTRPGLSSVGLGCYGLAILTSYGFCSGIGLVFSPLHSVIPFLIMGLGVDDMFVIMQSLDNLDAKGGTALWSIERKIAETMKLGGVAITITSFTDLIAFAIGAITVLPALKSFCLFCAVGIASVYFYTATIFVAFLSIDERRIRAKRNGMCPLIVHKDWTPNKISQREFGRELFEKLGWVLSNPFVQAFVLIATAVLGGFGIYGLSELKQEFNPIKFIPPESYLAGWFHANTEYFPKEGERVIVNIGDIDYNNELHKLENLVGALVNETEILSAVDSWYLEFRKFAVGKNLVEESDWFNLFQENREKFYTILTQFLFSPDGAPYRRRFNFVNDLICGEAAPTVLLSSIELTHRLFTSPSEWIPAMNRIKELVREANFSSRAFPTGTEYASWETDEVIGYELYQNMGISLLCVFLTVLLLIASIRACLLVLVCVVLSLVNVAGYMHFWGLTVDVISCVNLEIAIGLTVDYSAHVAHTFLIQKGSKSERMRSTLTEIGPAVLNGGFSTFLAFIFTVGSKSHVFISFFRIFMLVVLFGLYHGLIFLPVALALIGPEPHQKRKE-DDDEMNT-EEGKI 988          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|321463817|gb|EFX74830.1| (hypothetical protein DAPPUDRAFT_306990 [Daphnia pulex])

HSP 1 Score: 721.079 bits (1860), Expect = 0.000e+0
Identity = 391/951 (41.11%), Postives = 576/951 (60.57%), Query Frame = 0

Query:   80 YGKAVANRPGWFIFLCILIVSASGSGLMKFYSENVGHKLWISEASSSRKNVDWLWENYPPSFRFASLIFEAEDILVPEVIQSMYSIHKEIEAIKTVHGEGWRDNCQMVPVFKAPDISQILSFGRRKRDTQNITEPADGFETSFQDDFFSEDDGFGNVGLDDIPANTTDET-NGMLDMAEAFSADSYPQPYCDIVEGIETACFEMSILELWANDGSYDDQTDSDIASLTKRSIIDKLNQVNTSGVFLIQRNFSSFLSEITYDPTNGQIIAARATVMRWFGRMNGTEALHNSVKERN------EPITHGTLEFEGEMLKIMLNETGYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVFTA-IGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQE-ASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNAS--GRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGPGPNAVEIELDEHGINVIENGRVIATEL 1019
            +G  VA  P  +I   ++I +    GL+ F +EN   KLWI + S    N +WL E++P   R  S +   +D+L PE    M  +H  I  + + +G  W+D C  +PV   P+        RR++   N +E        F  D F  +        +DIPA   D      +D+            +CDIVEG+E AC E + L++W    +Y  +    I+ L+++ II+ +N V  S +  +Q  +S+ + +  Y+ + G I+ A + +++   R+N +   +++           +PI+   LE+EG  + ++ N T   +    Y  A RSFGD++ +TILGDV  L  G  +VF +V +MLG+FN VEQRAFLS  GI  V M I+ +YG+CSA G+F+GP+H ILPFLLLGIGIDDMFVIVQC++ L +EE    L  + G TM+HAGV+ITITS+TD  AF +G +TV+P+L+SFC+Y++VGI+ATY +Q +FFVAW+ +DQRRIE+ RDG  PC+ H NW P+ LS+ + L   F+  +   L K P K+ ++IL+  + GV  WG+VL+ QEF+  WF+P  TY++++F   + ++P  G    IY    +   + + +  L+D ++ +T IV  V+ W   F ++  K    D+         F++ L  + F+ +G K+R  F+F+K L+C    A  + ++  +FV + FSG +EHIPAM RIKE+  + +      +F  ++ ++ WETDE+I EELYRNL +A++CVF+TT  LI+N+    LVL+CV+L+LI V G MHFW LTIDTVS  NL++AIGLCVDY+AH+AH FM + GTRN+R   T+ +IGPAV +GGFSTFLAFI LA+S SHVF +FFKIF LVV +GLFHGL+F PV+LS++GP P  V    D      +E+  V A EL
Sbjct:   66 WGITVAKNPWTYIIASVVIAATCSIGLLNFTAENRPTKLWIPQDSGFVHNSEWLQEHFPNEIRLQSWVLVDDDVLRPETFLWMLELHDNITELASSNGYKWKDVCFKIPVVTEPETK------RRRKRQANSSEIESFGFEDFDFDGFDSN-------FEDIPAPALDPNFEPAIDLPIGI--------FCDIVEGLEEACLENNFLDIW----NYKKEV---ISKLSRQDIINAINSVERSNLTRVQ--YSNLIGQPRYNES-GHIVGAGSVLVQLLTRVNYSLITNDATSNDAGTGDLVDPIS---LEWEGAFVNLLHNFTVTLNNSNLYYMASRSFGDISSATILGDVRLLSAGICVVFIYVQLMLGKFNLVEQRAFLSLTGIASVAMAILVSYGLCSAFGVFYGPVHSILPFLLLGIGIDDMFVIVQCWSNLNKEEMKRSLPVRIGITMKHAGVSITITSVTDFAAFAVGATTVLPSLRSFCIYSAVGILATYLFQASFFVAWLTLDQRRIESHRDGMLPCFVHRNWKPSTLSRIEPLQVFFSRFLAKYLFKWPAKVFILILSAFLLGVNTWGSVLMRQEFNPLWFIPTSTYLSQYFSTIESHYPDNGQLASIYIQTTNLSSNLDQLEALIDTVRNETQIVSQVDDWFTGFKDFTAKRHAIDWSSQNMTDEQFSTYLKNYLFTQKGAKFRSNFKFEKSLECRSSIAPPISITNFDFVFRPFSGREEHIPAMRRIKEIAEEYSLEFGSHVFASARIFSSWETDEVIMEELYRNLAIAMVCVFVTTFILIANLFACLLVLLCVVLTLICVNGSMHFWGLTIDTVSCINLVLAIGLCVDYAAHVAHTFMTKTGTRNERAAATISSIGPAVFHGGFSTFLAFIFLANSDSHVFLTFFKIFVLVVAYGLFHGLLFFPVVLSLVGPAPFDVAAPPD------VEDKAVTAEEL 976          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1325316278|ref|XP_023339853.1| (patched domain-containing protein 3-like isoform X1 [Eurytemora affinis] >gi|1325316280|ref|XP_023339854.1| patched domain-containing protein 3-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 699.893 bits (1805), Expect = 0.000e+0
Identity = 375/931 (40.28%), Postives = 554/931 (59.51%), Query Frame = 0

Query:   66 KNGIISGMERFFFFYGKAVANRPGWFIFLCILIVSASGSGLMKFYSENVGHKLWISEASSSRKNVDWLWENYPPSFRFASLIFEAEDILVPEVIQSMYSIHKEIEAIKTVHGEGWRDNCQMVPVFKAPDISQILSFGRRKRDTQNITEPADGFETSFQDDFFSEDDGFG--NVGLDDIPANTTDETNGMLDMAEAFSADSYPQPYCDIVEGIETACFEMSILELWANDGSYDDQTDSDIASLTKRSIIDKLNQVNTSGVFLIQRNFSSFLSEITYDPTNGQIIAARATVMRWFGRMNGTEALHNSVKERNEPITHGTLEFEGEMLKIMLNETGYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKYF--DPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGP 992
            + G+I  +E+FF +YG+ VA  P   I +C L     G GL++F+ E     + I   S  R+N+D+L  N+P   R  S+++ A+++L P VIQ +Y   K+++ ++T   + ++D C  VP+ K P+     S   +K    N      G E    D+ F + +G    N GL     N   E    +D+  ++S   YP+PYC  V+  ETACFE++I+ELW + G Y + TD  IA LT+  I+DK+N  N S +FL ++NF   L ++ Y+   G+II A +  ++++  +N +         R E I   + +FEG ++K+     G P  + +Y N +R F +        D  KL +GY +VF +V +ML +FNCVEQR  LS  GI+ V MG+I AYGVCS  GLF+   H ++PFLLLGIGID++FVI Q FNTL      L L+ + G TM HAGVA+++T+ TDVIAF +G +TV+P L SFC+Y++V I A Y  Q T FVAWM++D++R+ A RDGC  C TH N+     S+K  L   F  +G  +T    KI +I+LT+      IWG + L QE+   W LPP++ IA+WF     Y+P  G+   +    +D   ++  +  LV  ++ +   ++ V +WT  F NY+N +      F  L  ++ +F  K +QF +SP+G  Y+  FEF + L+CG+ A  L+L  I F H  F    E IPAM  +++++     S   FPM+  Y  W+TD I+  EL+RN+G+A++C+F  TL  + +   S  V++CV+L+ +DV GFMH+W LTID  S N LII++GLCVD+ AHI H F++  GTR++RV   + NI PAVLNGGFS+ LA  LL  S+SH+F SFFKIFF++ TFGLFHGLV LPV+L+++GP
Sbjct:   38 QTGVIGSLEKFFIWYGELVATHPWKAILVCFLATLLGGLGLLRFHEEGDAASIVIPTNSEFRRNIDFLDNNFPREIRVHSVVYVADNVLTPSVIQYIYKQRKDLQNLET-DKKTFQDMCIRVPILKFPENGLGGSDSGKKSTVNNFENDDWGDEFKEFDEIFEQSEGGDPFNGGL----GNLFSEKEVDVDLLMSWSNTFYPEPYCSAVKATETACFELNIVELWGDQGHYSESTDLKIAFLTQADILDKINNKNISQIFLKEKNFKELLGDVKYNK-EGEIIGAGSVELKFYTTVNISSVKLYGTASRGEKIDQASYDFEGALIKLFTTREGMPRHMTSYVNVQRQFFESFVGQTFKDADKLVLGYVLVFIYVNLMLSKFNCVEQRIGLSVVGILSVAMGMILAYGVCSLFGLFYSAAHTVIPFLLLGIGIDNIFVITQAFNTLEASSVPLSLVKRIGQTMGHAGVAVSVTTFTDVIAFFVGSNTVLPGLASFCVYSAVSIFAIYALQVTHFVAWMSLDEKRMRAHRDGCLCCVTHKNFQSFEFSRKSILNKAFRMLGLLITNTVFKIFIILLTITFIVFGIWGALSLTQEYQPEWLLPPESEIAQWFGIKAIYYPSHGEPGFVMIPKIDMSTEFFKVEQLVQTLRSRDKNIEWVNTWTADFRNYVNTFTFSSLSFEELMQDEDFFRDKFTQFLYSPKGAAYQPYFEFGEELECGKPAPDLLLQAIPFGHLRFERSSEWIPAMQEVEQIVQDAGFSNYSFPMALTYINWKTDAIVGIELFRNIGIALICIFTATLCTLGSWRGSMFVMMCVLLTCVDVSGFMHWWGLTIDITSMNILIISVGLCVDFCAHIVHGFLIGKGTRHERVVFVMENIAPAVLNGGFSSLLALSLLVTSRSHIFISFFKIFFMICTFGLFHGLVTLPVVLTLIGP 962          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1228374474|ref|XP_021948457.1| (protein patched homolog 2-like [Folsomia candida])

HSP 1 Score: 676.396 bits (1744), Expect = 0.000e+0
Identity = 382/1007 (37.93%), Postives = 569/1007 (56.50%), Query Frame = 0

Query:   54 GRNPC--------LKCLDAMKNGIISGMERFFFFYGKAVANRPGWFIFLCILIVSASGSGLMKFYSENVGHKLWISEASSSRKNVDWLWENYPPSFRFASLIFEAEDILVPEVIQSMYSIHKEIEAIKTVHGEGWRDNCQMVPVFKAPDISQILSFGRRKRDTQNITEPADGFETSFQDDFFSEDDGFGNVGLDDIPANTTDETNGMLDMAEAFSADSYPQPYCDIVEGIETACFEMSILELWANDGSYDDQTDSDIASLTKRSIIDKLNQVNTSGVFLIQRNFSSFLSEITYDPTNGQIIAARATVMRWFGRMNGTEALHNSV--KERNEPITHGTLEFEGEMLKIMLN-ETGYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTL----TEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDG--CCPCWTHSNWTPNALSQKDYLGSVFTAIGHGLTKGP-IKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVN------ASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGPGPNAVEIELDEHGINVIENGRVIATELVETS---VNVNSGQNGN 1033
             RNP         +  L  +   I   ME+FF+ +G  VA  P   I  C+LI S  G G+  F  EN   KLWI   S   +N +W+   +P   R +  I  A+++LVPEV++ + +IH+ ++ I+   G+ W   C  +P+ K     +  S G+    + + T    G +    D   + DD F +      P  +    + +  + + F        YC IV  ++ AC+E+S  ELW+ D S  D       +LT+  II  +N    S +F    N+++           G++I+A + ++RW   +N T      V   +  E      LE+E + + +M N     P G+ TY    +S+ +++ +TIL D  +L +G+ I+FF+V IMLG+FN VEQR  L+ AG+  VG+  + +YG+CS   + +GP++ ILPFLLLGIGIDDMFVI+QC+N L       +  L + ++ G  M+HAGV+IT+TS+TDV AFG+G ST++P L+SFC+YA+VGIIA +  Q TFFVA  A+DQ+R+E +R+       +    + PN+ SQ+ YL ++F  I       P  KI VI++TL++ GV ++GN LL QEFD  WFLP D+Y++K+  K + Y+P  G    +  + V++   ++S   +++ +K  +D + ++E+W D F  Y N  FD + P  P   S F   + +F +SP G K+R  F+      CG+    L    + F  + F+GP+EHIPA+ ++K L+   N       S + F    G   WETD II+ ELYRN+ L++LCV +TT  L++N        +CV+L+LI+VGGFMHFW L+IDTVS  ++++AIGLCVDY+ HIA  FM   G+R  R +Q L+NIGPAVLNGGFSTFLA ILLA SKSH F SFFKIF +V+  GLFHGL+FLPV+LSI GP P       D    +  +  +V+  + ++ S   V V    N N
Sbjct:   20 ARNPTGTNSQTNSMTSLQKISLAITGSMEKFFYRWGFFVATHPLKAISCCLLITSICGLGIFNFTMENRPDKLWIPRNSEFAQNTEWIRATFPSPIRTSFAIVTADNVLVPEVMRVILNIHERVKQIEVTGGKNWESLCYKLPITKFLSKRKRRSIGQAYLHSNSTTNSHTGLQILSNDFDDAFDDEFFSENSTLKPQTSESGFSQLFVVPDEF--------YCSIVNELDAACWEVSPAELWSFDKSLID-------NLTQEEIIRVVNHELISPIFGRSVNYTAEFGGNIVRNDLGEVISAGSVLLRWSIGINQTLLQEGRVVTDKTGEVADDDCLEWELKWIDLMKNISKDMPPGINTYFVNSQSWMEISEATILDDGMQLAMGFIIMFFYVGIMLGKFNVVEQRPLLTIAGLSCVGLACVVSYGLCSLCFIVYGPVNKILPFLLLGIGIDDMFVIMQCWNNLFSSPLTNKSKLSIPERMGMAMKHAGVSITVTSLTDVAAFGVGASTIMPGLQSFCIYAAVGIIAVFLLQATFFVACFALDQKRVEDRRNAFFLWIKYKEDEFIPNSWSQESYLQTLFNRILAKYLFTPWAKITVILITLSMLGVTMYGNTLLRQEFDPMWFLPSDSYMSKYVEKRETYYPGTGYPSFLVANQVNWSESFQSFDRVLNDLKS-SDAIYNLENWYDDFKEYSNANFDTNIPNRPLTPSKFQKYMGKFLYSPAGSKHRPYFKSDGNFSCGESLPPLKTVVMPFRFRKFNGPQEHIPALRKVKSLLKTANMTFNHHESPKFFVTGTGLVAWETDNIINAELYRNVSLSLLCVLVTTFLLLANFRICAFCGICVVLTLINVGGFMHFWGLSIDTVSTIDIVLAIGLCVDYATHIALAFMTCTGSRESRAKQALKNIGPAVLNGGFSTFLAIILLAGSKSHAFQSFFKIFSVVIVCGLFHGLIFLPVLLSIFGPEPYPFAYSRDHELFDAFKPQKVVEMKPLQNSQVQVEVKDSSNAN 1010          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1228378754|ref|XP_021950763.1| (Niemann-Pick type protein homolog 1B-like [Folsomia candida] >gi|1215270797|gb|OXA57108.1| Niemann-Pick C1 protein [Folsomia candida])

HSP 1 Score: 667.537 bits (1721), Expect = 0.000e+0
Identity = 378/975 (38.77%), Postives = 558/975 (57.23%), Query Frame = 0

Query:   62 LDAMKNGIISGMERFFFFYGKAVANRPGWFIFLCILIVSASGSGLMKFYSENVGHKLWISEASSSRKNVDWLWENYPPSFRFASLIFEAEDILVPEVIQSMYSIHKEIEAIKTVHGEGWRDNCQMVPVFKAPDISQ-------------------ILSFGRRKRDTQNITEPA-DGFETSFQDDFFSEDDGFGNVGLD---DIPANTTDETNGMLDMAEAFSADSYPQPYCDIVEGIETACFEMSILELWANDGSYDDQTDSDIASLTKRSIIDKLNQVNTSGVFLIQRNFSSFLSEITYDPTNGQIIAARATVMRWFGRMNGTEA-LHNSVKE--RNEPITHGTLEFEGEMLKIMLNET-GYPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTL------TEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPC--WTHSNWTPNALSQKDYLGSVFTAIGHGLTKGPI-KILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNAS------GRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGPGP 994
            L+ +   I   +E FF+ +G  VA  P  FIF   +  +    G + F  EN   KLWI   S    + +WL  N+P   R +  IF AE++LVPEV+Q+++ IH+ +  I  + G+ W D C  +P+ K  + S+                   + S  R  R+       A D F+ +F +DFF+E      V  D   ++P  T                      YC +V  ++ AC+E++  ELW+ + S        I +LTK  I+  +N   TS +F  + N+++ L         G+II A A  +++   +N T   L  S+ +    E    GT  +E    ++M N +   P G++ Y    RS+GD+  S I  D   L +G+ ++F ++ IM+GRFN VEQR FLS AGI  VG  +I AYG+ +   L +GP++ ILPF+LLGIGIDDMFVI+QC++ L       + ++S+P  ++ G TM+HAGV+IT+TS+TDV AFG+G ST++P L+SFC+YA++GIIA +  Q TFFVA  A+DQ+R+E +R+G  P   ++ S +TP+  SQK Y  SVF      +   PI K +VI++TL   GV I+GN+LL+QEFD  WFLP D+++A +    +  +P  G+   I   NV++   +ES     +  K  +D +   E+W D F  Y N++F  D P  P +   F   + +F +SPRG K+R  F+ +    CG     + +  + F  + F+G  EHIP M+ IK ++A VN +      GR+ P S     WETDE+I+EELY+N+  + + V  TT  L++N+   F   +CVI +LI+VGG MHFW LTID +S  ++++A+GLCVDY+AHIA  FM   G+R  R    +R+IGPAVLNGGFSTFLA +LLA+S+SH F SFFKIF +V+ FGLFHGLVFLPV+L+++GP P
Sbjct:   18 LEKVSLAIQGSLENFFYRWGVLVATHPFKFIFGAFMFTALLALGFINFTQENRPDKLWIPRDSKFASDTEWLRNNFPSPLRESFAIFTAENVLVPEVMQAIFEIHERVGRISILDGKKWEDLCFKLPITKFNEASKYRRRRRRSVATTLPSREVLMTSENRTAREEDTFFGHAFDDFDNAFNEDFFNEVSSPHVVDWDPSIELPVET----------------------YCSMVNSLDEACWEVNPTELWSFNRSL-------IHNLTKDDIVQVMNYQLTSPIFGRKVNYTAELGGKITHNEKGEIIGAEAIRLKFVVAINQTAVDLGKSMSDFSTGETADEGTWLWEKAWTELMQNASKSLPRGIQVYYANGRSWGDITESLIFEDGMMLVVGFLVMFVYISIMIGRFNMVEQRPFLSIAGITCVGFAVIVAYGIGAMCFLLYGPVNKILPFMLLGIGIDDMFVIMQCWSNLYPSCLTKKCKQSIP--ERMGMTMKHAGVSITVTSLTDVAAFGVGASTIMPGLQSFCIYAAIGIIAVFLLQATFFVACFAVDQKRLEDRRNGFFPWIKYSESEFTPSEWSQKSYYQSVFEWFFFKIILTPIGKTIVILMTLGFLGVNIYGNLLLKQEFDPVWFLPDDSHLALFIKTRENNYPGTGNPAFIVAENVNWTASFESYDKFSEMFKS-SDEIYSFENWYDDFKIYANQHFGTDIPNKPLDPKLFQKYMGKFLYSPRGSKHRGFFKLEGNFSCGNNLPTIKMVMMPFRFRKFAGAAEHIPGMHHIKNIVASVNLTYNTGKHGRMIPWSPAMVTWETDELITEELYKNVLFSFISVLGTTFLLLANIRVCFFCGLCVIFTLINVGGMMHFWGLTIDMISFIDIVLAVGLCVDYAAHIALAFMSCSGSRAGRAEYAVRSIGPAVLNGGFSTFLAILLLANSQSHAFKSFFKIFTVVICFGLFHGLVFLPVLLTLIGPQP 960          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1325255661|ref|XP_023320290.1| (Niemann-Pick C1 protein-like [Eurytemora affinis])

HSP 1 Score: 657.522 bits (1695), Expect = 0.000e+0
Identity = 321/614 (52.28%), Postives = 434/614 (70.68%), Query Frame = 0

Query:  380 TLEFEGEMLKIMLNETG-YPDGLKTYANARRSFGDVAGSTILGDVGKLGIGYTIVFFFVMIMLGRFNCVEQRAFLSFAGIMGVGMGIITAYGVCSALGLFFGPMHGILPFLLLGIGIDDMFVIVQCFNTLTEEEKSLPLIDKFGATMQHAGVAITITSITDVIAFGIGGSTVIPALKSFCLYASVGIIATYFYQCTFFVAWMAIDQRRIEAKRDGCCPCWTHSNWTPNALSQKDYLGSVFTAIGHGLTKGPIKILVIILTLAITGVAIWGNVLLEQEFDITWFLPPDTYIAKWFVKNKEYFPFGGDRVIIYCHNVDYIHDYESIANLVDQIKQQTDIVDDVESWTDPFTNYMNKYFDPDFPVLPYNQSYFNSKLSQFFFSPRGGKYREQFEFKKPLQCGQEASQLMLSKINFVHKVFSGPKEHIPAMNRIKELIAQVNASGRIFPMSQGYTLWETDEIISEELYRNLGLAVLCVFITTLFLISNVITSFLVLVCVILSLIDVGGFMHFWNLTIDTVSANNLIIAIGLCVDYSAHIAHRFMVEHGTRNQRVRQTLRNIGPAVLNGGFSTFLAFILLADSKSHVFSSFFKIFFLVVTFGLFHGLVFLPVILSILGP 992
            T EFE  +  ++LN         K   N   SF  +A STILGDV    +G+ IVF +V IMLGR N VEQR FLS  G+M VG+ I  +YGVCS +GL FGP+H I+PFL+LGIGIDDMFV  QCF+ L ++ K   L ++ G  M++AG AITITSITD IAF IGG+TV+PAL+SFC++ ++GI+A YF Q T+F+AW ++DQRRIE KR+G  PC+ H N+TPN LSQ++ + ++F  +   + + P KI+++ LT A+ G++I+GNV L QEF+  WFLP D+YIA+W   N  Y+P  G++V ++  N+D   +  ++ ++  ++ +Q DI+ +++SW   F NYM   F     +    +  F+ +L+Q+ FSP G ++R  F+F   L CG+ +    LS ++F H++ SGP+E IPAMNR+K +I   N S R+FPM+QGY  WETDE+IS ELYRN+ LA+ C+FITT  L+ N     +VL+ V+++L++V GFMHFW LTIDTVS  NLIIAIGLCVDYSAHI H FMV  G+R +RV+  L NIGPAVLNGGFSTFLAFILLA SKSHVF+SFFKIFFLVV FGL++GL+ LP++LSI GP
Sbjct:   22 TEEFESALATLLLNTRDIVSKEFKVEVNVASSFAQIASSTILGDVTNFVVGFFIVFIYVNIMLGRLNFVEQRMFLSLMGLMSVGISIFFSYGVCSLMGLQFGPLHNIIPFLILGIGIDDMFVTTQCFSNLPKDGKERSLHERMGCAMKNAGCAITITSITDFIAFIIGGTTVLPALRSFCIFCAIGILAVYFLQATWFIAWFSLDQRRIEEKRNGSIPCYVHVNYTPNKLSQQNIVATMFQHLADIILRKPAKIMLLFLTAALFGISIYGNVELRQEFNPIWFLPSDSYIAQWHHFNSIYYPSRGEQVTVFIGNLDLPRELVNLEHMHSELLEQKDIIAEIDSWYLGFKNYMETNFLNGKSLFELEKDEFDRRLTQYLFSPGGSQHRFLFQFNHHLACGEPSPTPQLSILSFKHRLMSGPEEQIPAMNRVKTIIRNSNFSSRVFPMAQGYASWETDEVISVELYRNMSLALGCIFITTWILLFNFWACIMVLISVVVTLVNVAGFMHFWGLTIDTVSCTNLIIAIGLCVDYSAHITHTFMVNTGSRERRVKDALINIGPAVLNGGFSTFLAFILLATSKSHVFTSFFKIFFLVVLFGLYNGLILLPLLLSIFGP 635