K F77

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General

End Date: 

Monday, May 13, 2013 - 00:00

Experiment Batch ID: 

K

Start Date: 

Wednesday, April 3, 2013 - 14:49

Experiment Contact: 

Sussie Dalvin
Sample

Developmental Stage CV: 

PreAdult2
Adult

Number of Individuals (Start): 

36

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

UN8_g528

Primary target ID: 

EMLSAT00000003661, EMLSAT00000003661-699508 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Heidi Kongshaug

Antisense sequence: 

Aggcagagccaatgtgtctccatcaaaaaaaaagaagggcaatgaaaagaaaaagattgaaaaacatgtgcaaaacgggagtcgactctatcatcatcaacaaaccaaaaagcaccccaaagttcgccaacgtatcaactctggagaagggagttccgaagcggattccttatcatgggatgactacgatgttgctgctcacaaaaggagacagggaatacatacccttgagaacgttgagtctctcatctcaacttattcctctccttccctaagttcttcttccgcctcatccaaggagtccactccagagatgcaccaggaaggaaaacttttacttcaggaaatgaaggaactcagaaaggagattaagaaaatgggagatacttctgtccttgagaacaacactcactccaaggtgatacaactacttccaacggactattcaattgaggaactccaagaaatgatgaacaaaatggaggaagaggatcgttgttgggattc

Fragment Label ID: 

F77

Fragment concentration: 

600.00ng/ul

Fragment length: 

507bp

Fragment Producer: 

licelab

Primer Group: 

b1400
Validation

Efficacy of Knock Down: 

F>90.00%
Phenotype

Phenotype CV: 

Number of Individuals (End): 

19

Gallery Image: 

Image file: 

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F77 (UN8_g528): 12/21 animals did not carry eggstrings. Hatched copepodites looked normal.

RFK8 sectioned, looked fairly normal but dorsally the immature (posterior) part of the ovary looks abnormal. Egg anterior in GS (about to be vitellogenised) show abnormalities

The intestine seem to stain patchy especially in the anterior part (significant ?).

Subcuticular tissue appears normal

Some animals produced normal copepodites. It can be expected that all normal eggproduction would have ceased within one or 2 cycles.

The student Julia do the qPCR testing on sampled lice. 130902_F77

5' and 3' race have been done