Z F220

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General

End Date: 

Thursday, October 1, 2015 - 10:33

Experiment Batch ID: 

Z

Start Date: 

Thursday, August 27, 2015 - 13:44

Experiment Contact: 

Sussie Dalvin

Publication: 

Molecular characterization and functional analysis of components of the TOR pathway of the salmon louse, Lepeophtheirus salmonis (Krøyer, 1838)
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

30

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

TOR

Primary target ID: 

EMLSAT00000004214, EMLSAT00000004214-700061 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Heidi Kongshaug

Fragment Label ID: 

F220

Fragment concentration: 

600.00ng/ul

Fragment length: 

457bp

Sense sequence: 

aacggccttggatccagcaatatttgcaaacatcagcatgttggctcgagcagtaaaaggctctgttaagaacgacgttgctcctatgttagactccatgtttagtgttggacttagtccatctcttactatggcacttcatgagttagcgaggcatattccgtctctcaagaaagaaattgctgatggacttctcaaaatattatcacttatattaatgcaacaaccgtttagacacccaggaactcctattgcttccgcagctaattctcctactcctcctatgactctcgaaccaattccatccactgcatctgttgttcttggactacgcactctcggaacttttgattttgaaggtcactcattacttcaatttgtcagacattgtgctgatacatacctctataaagttgagaaagtggtccgacttgaagccgtcaagacatgctcctct

Fragment Producer: 

licelab
Validation

Efficacy of Knock Down: 

F>50.00%
Phenotype

Phenotype CV: 

Number of Individuals (End): 

17

Gallery Image: 

Image file: 

File rfz18.tif
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Image Quality: 

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Repeat injections of TOR. Take out samples at 10 and 20 days after injections + at end. Look at the varations.

15 days post injections: 70% downregulation

25 days post injections: 50 % downregulation

35 days post injections 20% downregulation