AP F367 EMLSAG00000005299

Submitted by Christiane Eichner on 29 May, 2018 - 12:27

Phenotype CV:

General

Experiment Batch ID:

AP

Experiment Contact:

Christiane Eichner

Start Date:

6 March, 2018 - 09:00

End Date:

10 April, 2018 - 09:00

Experiment Description:

EMLSAG1458 knock-down

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000005299, EMLSAT00000005299-701146 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

EMLSAG00000005299

Fragment description

Fragment length:

338bp

Fragment concentration:

600ng/ul

Sense sequence:

>F367 gtatgatgacggacatgctcaagggtaacattactaacatgttgccaatgattgtaattggaggatggatcaactggcatttctcaggctttgtcactaccaaaatcccttttcccctcactcttcgtttcaagcctatgcttcaaagaggcattgaactcatgagtctagatgcttcatgggtctcctccgcctcctggtactttcttaacgttttcggactacgatctatttatgacttagttctcggagaaaataatgttgcggatcaatcaagaatcatgcaggaccagatgatgatgcctgccaacggaatggctaccgactataaacaggcc

Fragment Label ID:

F367

Primer Group:

likb2502_T7_b2515

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Eichner

Validation

Efficacy of Knock Down:

F>16%

Phenotype

Number of Individuals (End):

24

Phenotype:

slightly shorter gonade and abdominal segment

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Testing the fragment for Mvp1 knock-down.

LsRXR

Submitted by Michael Dondrup on 11 April, 2016 - 12:18

Phenotype CV:

General

Experiment Batch ID:

LsRXR

Experiment Contact:

Christiane Eichner

Michael Dondrup

Frank Nilsen

Start Date:

13 March, 2015 - 11:54

End Date:

7 April, 2015 - 10:02

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

50

Developmental Stage CV:

Adult

Copepodid

Target description

Primary target ID:

EMLSAT00000001919, EMLSAT00000001919-697766 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsRXR

Target type:

protein_coding_primary_transcript

Fragment description

Fragment length:

301bp

Fragment Label ID:

RNAiFr1

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>60%

Phenotype

Number of Individuals (End):

38

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Background

Z F214

Submitted by Heidi Kongshaug on 3 September, 2015 - 09:21

Phenotype CV:

General

Experiment Batch ID:

Z

Experiment Contact:

Frank Nilsen

Start Date:

27 August, 2015 - 13:44

End Date:

1 October, 2015 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAG00000000426, EMLSAG00000000426-683192 (gene) Lepeophtheirus salmonis

Gene or target symbol:

426

Fragment description

Fragment length:

766bp

Fragment concentration:

600ng/ul

Sense sequence:

agtgggatttgcttgcaggagtgtataaatttagggtttccacctttttaaaaagtcttgtatttgaatccaaagaaaaaagagtgaccattgatcgttctctaataaaagactatggaatcagatcaatcaataagcgaaaatattatttcttctgccttgggagtaggagcccctttggtaagggggaaattatccttaatagttttgattttgatttggatgaatttaacaattatgctctatcactccatgctaacaaaagtttaagcttaaaacgatcagtttctcgaaaaaatggaagaattaagtcaagaacaagttcagaatctgatgttaccaaacaagtttccaatttatccaaactgttttcaaaattgtttaagagcaaaaaagataggaagaaaaaattgttgaaggatagggattcgttgacatctaaagaggattactcagttgttgatgaatattcggaaatcttaccaagtggctatagtggtcaaacttatctctgcacttctgaagatttatcggatagtgaatctatgggkcttgtattkccttctccygattttwagacaaaatcctgcaaatcctttaaaaaaaataatcctaaaaaccatctcaaatggcatgaacagaatgaaatttactccgagtacgctccaagttatttgattcctgttgagcatgaaacaaaacatgaagttcttgtaaaagaaacaggagatgtctacaccctaccaatgacaaagaaaaagccaag

Fragment Label ID:

F214

Primer Group:

b3309

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Phenotype

Number of Individuals (End):

19

Phenotype:

Many bad and short eggstrings. Check hatching numbers

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Unknown function of gene.

Downregulation not verified by qPCR.

Z F220

Submitted by Heidi Kongshaug on 3 September, 2015 - 08:53

Phenotype CV:

General

Experiment Batch ID:

Z

Experiment Contact:

Sussie Dalvin

Start Date:

27 August, 2015 - 13:44

End Date:

1 October, 2015 - 10:33

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

30

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000004214, EMLSAT00000004214-700061 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

TOR

Fragment description

Fragment length:

457bp

Fragment concentration:

600ng/ul

Sense sequence:

aacggccttggatccagcaatatttgcaaacatcagcatgttggctcgagcagtaaaaggctctgttaagaacgacgttgctcctatgttagactccatgtttagtgttggacttagtccatctcttactatggcacttcatgagttagcgaggcatattccgtctctcaagaaagaaattgctgatggacttctcaaaatattatcacttatattaatgcaacaaccgtttagacacccaggaactcctattgcttccgcagctaattctcctactcctcctatgactctcgaaccaattccatccactgcatctgttgttcttggactacgcactctcggaacttttgattttgaaggtcactcattacttcaatttgtcagacattgtgctgatacatacctctataaagttgagaaagtggtccgacttgaagccgtcaagacatgctcctct

Fragment Label ID:

F220

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>50%

Phenotype

Number of Individuals (End):

17

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Repeat injections of TOR. Take out samples at 10 and 20 days after injections + at end. Look at the varations.

15 days post injections: 70% downregulation

25 days post injections: 50 % downregulation

35 days post injections 20% downregulation

B F2+F3 (IRP1+IRP2)

Submitted by Christiane Trösse on 29 April, 2015 - 16:21

Phenotype CV:

General

Experiment Batch ID:

B

Experiment Contact:

Christiane Trösse

Start Date:

19 March, 2012 - 09:00

End Date:

18 April, 2012 - 09:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000007801, EMLSAT00000007801-703648 (mRNA) Lepeophtheirus salmonis

EMLSAT00000000118, EMLSAT00000000118-695965 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsIRP1B

LsIRP1A

Fragment description

Fragment length:

441bp

Fragment concentration:

600ng/ul

Sense sequence:

ggtgtgagtccgagtccacagcgattccaattaaattaatgaaagaagggccctcatcagggactatggaagatgaagaccttctcaaggatttccaagttggggaaagaagctacaaatattacgacgtaacccaaatccagggctatgaggaactcccttactccttgcgtgttctcctggagtgtgccgttcgaaagcgttcggaaacgcaaggacaagtctggaaggatgtttatgaacggattttgaggcgagttcttcttcaggatttcacaggagttccagcattgatcgatttagcagcgatccgtgaatctgtatccaagtccggtggttgtccggatgtggtggacagtctttgtccagcagatctggttgtagatcactgtgttcaagtggactacaaccaaatcaagcagcaactgtcaagtccgcctg

Fragment Label ID:

F3

F2

Fragment Mixture Formula:

1:1 mixture, resulting in 300 ng/ul for each of the fragments

Primer Group:

b640

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>44%

Phenotype

Number of Individuals (End):

23

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Knockdown of LsIRP1A+LsIRP1B (formerly called IRP1 and IRP2) in preadult female lice. Slightly shorter egg strings. Knockdown 62% for IRP1 and 44% for IRP2. Described in the fragment fields is only F3, see experiment A for F2.

A F3 (IRP2)

Submitted by Christiane Trösse on 29 April, 2015 - 15:16

Phenotype CV:

General

Experiment Batch ID:

A

Experiment Contact:

Christiane Trösse

Start Date:

23 January, 2012 - 09:00

End Date:

24 February, 2012 - 09:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

39

Developmental Stage CV:

PreAdult2

Adult

Target description

Primary target ID:

EMLSAT00000007801, EMLSAT00000007801-703648 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

LsIRP1B

Fragment description

Fragment length:

441bp

Fragment concentration:

600ng/ul

Sense sequence:

ggtgtgagtccgagtccacagcgattccaattaaattaatgaaagaagggccctcatcagggactatggaagatgaagaccttctcaaggatttccaagttggggaaagaagctacaaatattacgacgtaacccaaatccagggctatgaggaactcccttactccttgcgtgttctcctggagtgtgccgttcgaaagcgttcggaaacgcaaggacaagtctggaaggatgtttatgaacggattttgaggcgagttcttcttcaggatttcacaggagttccagcattgatcgatttagcagcgatccgtgaatctgtatccaagtccggtggttgtccggatgtggtggacagtctttgtccagcagatctggttgtagatcactgtgttcaagtggactacaaccaaatcaagcagcaactgtcaagtccgcctg

Fragment Label ID:

F3

Primer Group:

b640

Fragment Producer:

licelab

Fragment Producer Contact:

Christiane Trösse

Validation

Efficacy of Knock Down:

F>45%

Phenotype

Number of Individuals (End):

23

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Knockdown of LsIRP1B (formerly called IRP2) in preadult female lice. Slightly shorter egg strings caused less offspring.

D F32/F33

Submitted by Heidi Kongshaug on 5 February, 2015 - 08:46

Phenotype CV:

General

Experiment Batch ID:

d

Experiment Contact:

Sussie Dalvin

Start Date:

24 August, 2012 - 13:49

End Date:

3 October, 2012 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

40

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000002599, EMLSAT00000002599-698446 (mRNA) Lepeophtheirus salmonis

EMLSAT00000003443, EMLSAT00000003443-699290 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

g18363_TSC2

NLG3408_TSC1

Fragment description

Fragment concentration:

600ng/ul

Fragment Label ID:

F

Primer Group:

LS_TSC2-F_b872

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>30%

Phenotype

Number of Individuals (End):

14

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RD71.tiff

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TSC1+2, short eggstrings (15853), not the best appetite jlkj jkljlkj jk kl lj l jklkj

D F33

Submitted by Heidi Kongshaug on 4 February, 2015 - 13:53

Phenotype CV:

General

Experiment Batch ID:

d

Experiment Contact:

Sussie Dalvin

Start Date:

24 August, 2012 - 13:49

End Date:

3 October, 2012 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

40

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000002599, EMLSAT00000002599-698446 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

g18363_TSC2

Fragment description

Fragment length:

432bp

Fragment concentration:

600ng/ul

Sense sequence:

aaaatgggcattcagcgacttggttgattggcacaatgcttataactgtttctacgagctgttgtacccatattgcaatgagaaatggtgtatgtgatagatgttatttttattgcattcgagatggaactttaagtaaggatgatattactaaaaattcggatagaagaagacacatgtcggatttttactccggtacaaataagagtcagttatcaaacttaagaagtgataaggaacttcataggcacacttctctggagagaatggagtcttctggagatgattatagagtaaaaaaagtgctacctgaaacatgcagttgttggtgctctggctgggctgaaatttacattcggcgtccaagtggggatgtatcctggatgtgtagagtgcaaaatactcagttgcttagtgcgcatacaccctcaa

Fragment Label ID:

F33

Primer Group:

LS_TSC2-F_b872

Fragment Producer:

licelab

Fragment Producer Contact:

Heidi Kongshaug

Validation

Efficacy of Knock Down:

F>72%

Phenotype

Number of Individuals (End):

11

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Tsc2, a bit short eggstring TSC-2: Significant downregulation (but only with primers outside the dsRNA fragments, both tested)

D F31

Submitted by Heidi Kongshaug on 4 February, 2015 - 13:36

Phenotype CV:

General

Experiment Batch ID:

d

Experiment Contact:

Sussie Dalvin

Start Date:

24 August, 2012 - 13:31

End Date:

3 October, 2012 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

female

Number of Individuals (Start):

40

Developmental Stage CV:

PreAdult2

Target description

Primary target ID:

EMLSAT00000004214, EMLSAT00000004214-700061 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

TOR

Fragment description

Fragment length:

505bp

Fragment concentration:

600ng/ul

Sense sequence:

tgtctggggactcagcctccaagatgggaggtatcctggccatagttgctctactcaacgcggatgtttgtaacacggggagtcgaattcatcgatttggaaattatcttcaaaacaattgtttaccgggagggaactccgtgacggatcctgtggtgattgccctggctactaaagccattggccgacttactcaagtatatggtagctacaaggctaatttacgctccgacttcattaattcactcgtcaagaaatcatttgagtggctttccaaccccaatgatcgcaatgatctcaagtggtacgctgctgttctcattctaagagaaatagccatttgtgagcccactttctttttccagaacgtaccaacgttttttgatcttatcttcattcctgtcagagactcaaaaccatttcttagagaatcagccgttaatgcgctacgagctggtctcatcgttactgcccaaagagaaaccaccaaacaatccagacgcca

Fragment Label ID:

F31

Primer Group:

LsTOR_f_b807

Validation

Efficacy of Knock Down:

F>0%

Phenotype

Number of Individuals (End):

14

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TOR, short eggstrings (14763), stunted growth of genital segment (90% of control), 2 ødipus eggstrings TOR: Significant downregulation (but only with primers outside the dsRNA fragments, both tested) NB! No downregulation BUT upregulation -95%