LsHSCARB on free living stages

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General

End Date: 

Tuesday, August 2, 2016 - 10:00

Experiment Description: 

Knock-down evaluation of primers

Experiment Batch ID: 

F289

Start Date: 

Tuesday, July 26, 2016 - 09:55

Experiment Contact: 

Erna Irene Heggland

Publication: 

A scavenger receptor B (CD36)-like protein is a potential mediator of intestinal heme absorption in the hematophagous ectoparasite Lepeophtheirus salmonis
Sample

Developmental Stage CV: 

Nauplius I
Copepodid

Number of Individuals (Start): 

300

Sex: 

unknown

Organism: 

Lepeophtheirus salmonis
Target description

Primary target ID: 

EMLSAT00000005382, EMLSAT00000005382-701229 (mRNA) Lepeophtheirus salmonis
EMLSAG00000005382, EMLSAG00000005382-688148 (gene) Lepeophtheirus salmonis
Fragment description

Fragment Label ID: 

F289

Fragment concentration: 

10.00ng/ul

Fragment length: 

731bp

Sense sequence: 

AGCGGATAAACTCGATGGCTTGCCAAAAACAGTAAAAGATATTTGTTTTGAAATTATAAATAATGGTAATGAAGCTCTAGGTATTAAGGCCGAAGATTTATTTATTACTGAAGAAGTTGACAAAATCCTATACACGGGCTTCGACTCTAAGTCTGCTGCTATCTTTGATAAATTGGATACATTTCTTATGCTCTTGTTAGAAGTCATACAAGAATCCTTGGAGTTGGATATACCAATTAAAGCAAAAGACTTTGAAAACATAATCAAAATCATTAGTCCTGCACAGCTTTCAGAAGGGACTTTTGCTTTTTTCAAAGGTAAAAATGCAACCAAATTGCAGAATTACTATACCATTGAAAACGGACGGTTCGATAAAGAAAGTTTTATGAATATTGTTGAGTTCAATGGTAAGAATAAATTACCTGAGGCTTKGTGGCCTAATGTAGCCACAAGTATAACGGGKCAACTTTCAAGTGAGGGTGGCTCATGCCATAGAATCTATGGAACGGATGGGACTCAATTTCCACCGTTTTTATTTAACAAGAAAAAATTTCCTTTGTGGATGTTTGTGGGAGAGCTTTGTAGAACAATATATGTGGAGTTTGAGTCCGAAGTGGAAGTGGAAGGAGGGATCACAGCCTATCGTTATGGAGTAGGGAAGAGAGTTTTCTCAATGAGTAACCCTGAAAACTTTTGTTATTGTCAAGAATTTTTCTCATGCGCCAAGCAAA
Validation

Efficacy of Knock Down: 

F>99.00%
Phenotype

Phenotype CV: 

Phenotype: 

none

Number of Individuals (End): 

250
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Testing the efficiency of RNAi fragment in freeliving stages. Non-parasitic stage is not feeding and therefore no phenotype was expected.