no visible phenotype

AV F353

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AV

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

14 March, 2019 - 09:00

End Date: 

24 April, 2019 - 09:00

Experiment Description: 

Repeat of downregulation of 4874 Var 2_2
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

4874_Var2_2

Target type: 

External DBref: 

Fragment description

Fragment length: 

342bp

Fragment concentration: 

600ng/ul

Sense sequence: 

Antisense sequence: 

catgaatgagtctggcttaaacttaaaatacccctcgagttatcatgaagaaattaatccaactgtcgcctctgttttcgttgctgctgcatacagatttggccattccatgattcaaaggaatgtgaatcgattggctataaaaaaatatagctatagcagtaattattccttatttaaggacttttttaatacggcaaattacgaggataggaatgggctaggaatagaagaaattttatatgggttgacctatttcccagcacaatctgcagaccgttttgttgtaaaagaagtaactaattatttgttcaataatggaaagccgtatggagcagatct

Fragment Batch ID: 

Fragment Label ID: 

F353

Fragment Mixture Formula: 

Primer Group: 

likb2502_T7_b2515

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

14

Phenotype: 

normal
Upload

Image file: 

File RAV40.tif
File RAV38.tif

Gallery Image: 

Image Quality: 

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Testing the fragment for Mvp1 knock-down.

AP F353

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AP

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

7 March, 2018 - 13:26

End Date: 

10 April, 2018 - 10:54

Experiment Description: 

Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

4874 Var2_2

Target type: 

External DBref: 

Fragment description

Fragment length: 

342bp

Fragment concentration: 

600ng/ul

Sense sequence: 

Antisense sequence: 

catgaatgagtctggcttaaacttaaaatacccctcgagttatcatgaagaaattaatccaactgtcgcctctgttttcgttgctgctgcatacagatttggccattccatgattcaaaggaatgtgaatcgattggctataaaaaaatatagctatagcagtaattattccttatttaaggacttttttaatacggcaaattacgaggataggaatgggctaggaatagaagaaattttatatgggttgacctatttcccagcacaatctgcagaccgttttgttgtaaaagaagtaactaattatttgttcaataatggaaagccgtatggagcagatct

Fragment Batch ID: 

Fragment Label ID: 

F353

Fragment Mixture Formula: 

Fragment Producer: 

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

14

Phenotype: 

Upload

Image file: 

File RAP34.tif
File RAP35.tif

Gallery Image: 

Image Quality: 

0
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Knockdown of 4874 var 2_1

AV F352

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AV

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

14 March, 2019 - 09:00

End Date: 

24 April, 2019 - 09:00

Experiment Description: 

Repeat of downregulation of 4874 Var 2_1
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000004874, EMLSAT00000004874-700721 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

4874_Var2_1
Fragment description

Fragment length: 

322bp

Fragment concentration: 

600ng/ul

Sense sequence: 

tcaagcgacgaggaattattgctactcaaggaagaaaattcttacttaatgaagagtacgctgactgatgatggaaaagaactccttcccaaagatggtaaaggtagttttgtgggaggagacgctagagctcttctaaccactggtttggcttcagttcataccttattcgttcgggaacataatcgaatatcaaggaaacttgctcagatgaatccttcatacacaaacgaaactgtttttcaagaaactcggcgaatattgggagcaatgatgcaaaatattgtttatagtgaatttcttccgacgttattaggggaaa

Fragment Label ID: 

F352

Primer Group: 

likb2502_T7_b2515

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

11

Phenotype: 

normal
Upload

Image file: 

File RAV11.tif
File RAV12.tif
File RAV13.tif

Gallery Image: 

Image Quality: 

0
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Testing the fragment for Mvp1 knock-down.

AV F353

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AV

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

14 March, 2019 - 09:00

End Date: 

24 April, 2019 - 09:00

Experiment Description: 

Downregulation of variant 2_2
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAG00000004874, EMLSAG00000004874-687640 (gene) Lepeophtheirus salmonis
Fragment description

Fragment length: 

670bp

Fragment concentration: 

600ng/ul

Sense sequence: 

tcaagcgacgaggaattattgctactcaaggaagaaaattcttacttaatgaagagtacgctgactgatgatggaaaagaactccttcccaaagatggtaaaggtagttttgtgggaggagacgctagagctcttctaaccactggtttggcttcagttcataccttattcgttcgggaacataatcgaatatcaaggaaacttgctcagatgaatccttcatacacaaacgaaactgtttttcaagaaactcggcgaatattgggagcaatgatgcaaaatattgtttatagtgaatttcttccgacgttattaggggaaaaaagcatgaatgagtctggcttaaacttaaaatacccctcgagttatcatgaagaaattaatccaactgtcgcctctgttttcgttgctgctgcatacagatttggccattccatgattcaaaggaatgtgaatcgattggctataaaaaaatatagctatagcagtaattattccttatttaaggacttttttaatacggcaaattacgaggataggaatgggctaggaatagaagaaattttatatgggttgacctatttcccagcacaatctgcagaccgttttgttgtaaaagaagtaactaattatttgttcaataatggaaagccgtatggagcagatcttg

Fragment Label ID: 

F353

Primer Group: 

likb2502_T7_b2515

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

11

Phenotype: 

normal
Upload

Image file: 

File RAV41.tif
File RAV38.tif

Gallery Image: 

Image Quality: 

0
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Testing the fragment for Mvp1 knock-down.

AV F352

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AV

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

14 March, 2019 - 09:00

End Date: 

24 April, 2019 - 09:00

Experiment Description: 

Downregulation of Var 2_1
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAG00000004874, EMLSAG00000004874-687640 (gene) Lepeophtheirus salmonis
Fragment description

Fragment length: 

670bp

Fragment concentration: 

600ng/ul

Sense sequence: 

tcaagcgacgaggaattattgctactcaaggaagaaaattcttacttaatgaagagtacgctgactgatgatggaaaagaactccttcccaaagatggtaaaggtagttttgtgggaggagacgctagagctcttctaaccactggtttggcttcagttcataccttattcgttcgggaacataatcgaatatcaaggaaacttgctcagatgaatccttcatacacaaacgaaactgtttttcaagaaactcggcgaatattgggagcaatgatgcaaaatattgtttatagtgaatttcttccgacgttattaggggaaaaaagcatgaatgagtctggcttaaacttaaaatacccctcgagttatcatgaagaaattaatccaactgtcgcctctgttttcgttgctgctgcatacagatttggccattccatgattcaaaggaatgtgaatcgattggctataaaaaaatatagctatagcagtaattattccttatttaaggacttttttaatacggcaaattacgaggataggaatgggctaggaatagaagaaattttatatgggttgacctatttcccagcacaatctgcagaccgttttgttgtaaaagaagtaactaattatttgttcaataatggaaagccgtatggagcagatcttg

Fragment Label ID: 

F352

Primer Group: 

likb2502_T7_b2515

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

11

Phenotype: 

normal
Upload

Image Quality: 

0
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AV F350

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AV

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

14 March, 2019 - 09:00

End Date: 

24 April, 2019 - 09:00

Experiment Description: 

Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAG00000009839, EMLSAG00000009839-692605 (gene) Lepeophtheirus salmonis

Target type: 

External DBref: 

Fragment description

Fragment concentration: 

600ng/ul

Sense sequence: 

Antisense sequence: 

Fragment Batch ID: 

Fragment Label ID: 

F350

Fragment Mixture Formula: 

Primer Group: 

likb2502_T7_b2515

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

20

Phenotype: 

normal
Upload

Image file: 

File RAV11.tif
File RAV12.tif
File RAV13.tif

Gallery Image: 

Image Quality: 

0
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Testing the fragment for Mvp1 knock-down.

AU F336

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AU

Experiment Contact: 

Andreas Borchel

Start Date: 

1 February, 2019 - 08:35

End Date: 

7 March, 2019 - 08:23

Experiment Description: 

injection into pradult males
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

male

Number of Individuals (Start): 

30

Developmental Stage CV: 

Adult
Target description

Primary target ID: 

EMLSAT00000001316, EMLSAT00000001316-697163 (mRNA) Lepeophtheirus salmonis
EMLSAT00000011944, EMLSAT00000011944-707791 (mRNA) Lepeophtheirus salmonis
EMLSAT00000000699, EMLSAT00000000699-696546 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F336

Fragment Mixture Formula: 

33% of each, in total 600 ng/ul

Fragment Producer: 

licelab

Fragment Producer Contact: 

Andreas Borchel
Phenotype

Number of Individuals (End): 

6
Upload

Image Quality: 

0
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Knockdown of a testis-specific ion-pump in pre-adult1 males, checking reproduction in adults. Many males were lost, and no kncokdown observed.

AV F0

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AV

Experiment Contact: 

Christiane Eichner

Start Date: 

14 March, 2019 - 09:00

End Date: 

24 April, 2019 - 09:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

CPY185 (negative control), CPY185 (PCR_product) Gadus morhua

Gene or target symbol: 

CPY185
Fragment description

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F0

Primer Group: 

likb2502_T7_b2515

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

13

Phenotype: 

normal
Upload

Image Quality: 

0
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Testing the fragment for Mvp1 knock-down.

AV F0 extra

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AV

Experiment Contact: 

Christiane Eichner

Start Date: 

14 March, 2019 - 09:00

End Date: 

24 April, 2019 - 09:00

Experiment Description: 

Extra control to Mvp1 and Mvp2/3 knock-down
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

CPY185 (negative control), CPY185 (PCR_product) Gadus morhua

Gene or target symbol: 

CPY185
Fragment description

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F0

Primer Group: 

likb2502_T7_b2515

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

17

Phenotype: 

normal
Upload

Image file: 

File RAV88.tif

Image Quality: 

0
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Testing the fragment for Mvp1 knock-down.

AV F364 (F168+F169)

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AP

Experiment Contact: 

Christiane Eichner

Start Date: 

14 March, 2019 - 09:00

End Date: 

24 April, 2019 - 09:00

Experiment Description: 

Mvp1 and Mvp2/3 knock-down
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis
EMLSAT00000005383, EMLSAT00000005383-701230 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

UN53_5145, UN52_5383
LsMvp1, LsMvp2/3

Target type: 

UN53_5145
UN52_5383
Fragment description

Fragment length: 

576bp

Fragment concentration: 

600ng/ul

Sense sequence: 

>F169 cacatggagacgaagaagtgcgattggagagagatccgtttccattatatccaggagaagagttaaaggtcaaggtcacaccattggcaatcgttcatacctcaaatgcccttttattaaaagttataagaaatttcacggacgaaaatgatgttaaacgtgtggctggagatttatttttgtttgagggacctggaacttatattccaaggaaagaggtcgaggttcttaaaaccattacagcacaagttatccatgaaaatgaagcccttaaattaagtgcaagtcgtgaaaccatagatcgatccggaattaaacacgttgcaggtgaagaatggctcataagaaagcctggagcttatttgcctttggcttatgaaaatattgtggaagttgttaaggcacatataccccttccagatgtcgcaattttggtaagagcaaaggcaactttcaaggacaagtttggtgttgagagaaaaaatggagacaaatacttgattacctttgaagatacatcagcattcattcctgaggtacaagaagaaataattggcacagtggatgctatcacac >F168 cctcccacccgaatggtcgtaatacctccaggatcttattgtgttgtatcaaaccctgttgtaacaaaagatggtactattgagaaagatcgctttggccaaattaagttatctcatggagatgaagaaattcgattaaaaagagatccttttccattatatccaggagagaaattgaaagttgaagtgacgcctttaacaatcgtacattcatcaagtgcacttcttttaaaagttatcagaaattttactgatgaagacaaaactgagcgtsttgctggagatttatacttgttcgaaggtccaggaacttattttcctagaaaagaggtggaagttgttaaaactattactgctacagtcatacatgagaatgaagctttaaaactgagtgctgctcgtgaaactttagacagatctggttgtaagagagtggcaggggagg

Fragment Label ID: 

F364

Fragment Mixture Formula: 

F169+F168

Primer Group: 

likb2502_T7_b2515

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

15

Phenotype: 

normal
Upload

Image file: 

File RAV104.tif

Image Quality: 

0
No votes yet

Testing the fragment for Mvp1 knock-down.

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