ExpH - EMLSAT000000010949

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General

End Date: 

Wednesday, May 16, 2018 - 09:54

Experiment Description: 

Nauplia I was given dsRNA 8 days prior to infestation. Fish (around 100g) were infested with 80 cops/Fish and skin with and without lice were sampled 3 days after infestation.

Experiment Batch ID: 

ExpH

Start Date: 

Sunday, May 13, 2018 - 09:51

Experiment Contact: 

Aina-Cathrine Øvergård
Sample

Developmental Stage CV: 

Copepodid
Copepodid

Number of Individuals (Start): 

640

Sex: 

both

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

Trypsin

Target type: 

mRNA

Primary target ID: 

EMLSAT00000010949, EMLSAT00000010949-706796 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Aina-Cathrine Øvergård

Fragment Label ID: 

F233

Fragment concentration: 

14.00ng/ul

Fragment length: 

446bp

Sense sequence: 

gggacaaacctcggtcagcgataaaagagggagmaatgttttaaaacgtacaagactccaggttctccagaactctcatcagtattgtattaatggagcttctcttgggaaaccaccatggataaagcaaatgtgcgcctatgctgaaggaacagattcttgtggaggggatagtggaggmccacrcacattacctcaaaatgggagatgtgstctagtagggyttgtatcttatgggttggaatgtgccctgacttaccatgctggagtatatactcgtgtctctgaatatttgttttggataaaacatctagcaaaagatggaggctgtttcggtggagggaccgaaatgaaaccaaaatattgtgatatgcgctgtttgtggggacatgtcactgcaacatactacattactaataataggcatgaagttaagtgcgaccagg

Fragment Producer: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>93.00%
Phenotype

Phenotype CV: 

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Experiment H - Experiment to sheck immune modulatory effect of salivary gland proteins.