AL Hormone receptor 3 (HR3)

Submitted by on

General

End Date: 

Friday, October 6, 2017 - 10:00

Experiment Description: 

Knockdown of the ortholog of the nuclear receptor HR3. Injection of pre-adult 2 females

Experiment Batch ID: 

RNA_AL

Start Date: 

Monday, August 28, 2017 - 10:00

Experiment Contact: 

Joakim Brunet
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

30

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

EMLSAG00000000733

Target type: 

mRNA

Primary target ID: 

EMLSAT00000000733, EMLSAT00000000733-696580 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Joakim Brunet

Fragment Label ID: 

F341

Fragment Batch ID: 

F341

Fragment length: 

441bp

Sense sequence: 

gccta Gcaccattcgagcaagaaatctcattgcaaaacaaacaaacaaaaaggattcatagaata Aatagaaaaaggaccctacaaggcaaaaaaaacagcaactattttttacaaattgtttcg Tactcgtcgatgcaaaatcagaaatattatacgaatttgtcctcaatggattggatatca Ggctatcgtcttccatccaattcggatttaaagtgtgatgatgatcaccatggcggcggg Gcaggagtacagcatgcatctgttgtcggcggaccaacaggcggagcactaggatctggc Tcttccggtcccctcacaaacaacaccaattccaactcttcctcaccctccatgctaggc Atgtgggcagcagctgtggccaatacacctcttaaagtggaaacagctgatcatcatgga Aatggatctctggtgg

Fragment Producer: 

licelab
Validation

Efficacy of Knock Down: 

F>90.00%
Phenotype

Phenotype CV: 

Phenotype: 

No eggstrings. Disorganization of eggs in genital segment

Number of Individuals (End): 

17

Gallery Image: 

Image file: 

Image icon RFAL16_HR3_s109_s2_genital_2.5x_AX10_ScopeA1.jpeg
Image icon RAL72.jpg
Upload

Image Quality: 

0
No votes yet

Knockdown of HR3 in pre-adult 2 females. Resulted in no eggstring production. Sections reveals disorganized eggs in genital segment. Ovaries look normal.