ExpH - EMLSAT000000009293

Submitted by Aina-Cathrine Ø... on 14 June, 2018 - 11:08

General

End Date:

Wednesday, May 16, 2018 - 09:54

Experiment Description:

Nauplia I was given dsRNA 8 days prior to infestation. Fish (around 100g) were infested with 80 cops/Fish and skin with and without lice were sampled 3 days after infestation.

Experiment Batch ID:

ExpH

Start Date:

Sunday, May 13, 2018 - 09:51

Experiment Contact:

Aina-Cathrine Øvergård

Sample

Developmental Stage CV:

Copepodid

Number of Individuals (Start):

640

Sex:

both

Organism:

Lepeophtheirus salmonis

Target description

Gene or target symbol:

Unknown

Target type:

mRNA

Primary target ID:

EMLSAT00000009293, EMLSAT00000009293-705140 (mRNA) Lepeophtheirus salmonis

Fragment description

Fragment Producer Contact:

Aina-Cathrine Øvergård

Fragment Label ID:

E9293_V1+V2

Fragment concentration:

20.00ng/ul

Fragment length:

331bp

Sense sequence:

attcttaatgaagaatctggtgagggaaatgtcacagtcaccgtggtagatacaatggatagaagttcctttgtcatgaactccgatgatgagagtgctggtcttccagttccccttagtgaagtcgtggaaacccccaacaatgactatataatgaataatcctatcgttttaaatgagaaaaacgatgttattctgaatgacgatgttattcttagtggtgaagaccttactctgattgaaaatcccactttatcattggatgatcttctagttgaagaagaagaaagtgatgatgttattttgaactaaatgtcacaatatctttctg

Fragment Producer:

Aina-Cathrine Øvergård

Validation

Efficacy of Knock Down:

F>95.00%

Phenotype

Phenotype CV:

no visible phenotype

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Experiment H - Experiment to sheck immune modulatory effect of salivary gland proteins.

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