BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. L. salmonis genes
Match: EMLSAG00000006171 (supercontig:LSalAtl2s:LSalAtl2s336:651240:657086:1 gene:EMLSAG00000006171 transcript:EMLSAT00000006171 description:"maker-LSalAtl2s336-augustus-gene-6.36")

HSP 1 Score: 652.899 bits (1683), Expect = 0.000e+0
Identity = 300/389 (77.12%), Postives = 338/389 (86.89%), Query Frame = 0

Query:  592 EVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            E LPK+K+DLVAKIK+LR  L + QPQ+G+C LEVSRQEVFE+SYRQIVKMR K ++K+L +KFR E+GLDYGG+AREWL+LLSHEMLNPYYGLFQYS +D+YTLQINPDS++NPDHLSYFHFVGRIIGMAVFHGH IDG FTTPFYKM+LNKPINLDDIE VDP+LH SL W+L NDI+GI+D+ FSVEHEAFGL +  ELK+ GKD+ V E NK EYVKLYV +RWC GIEQQFL+LQKGFNELIPQ LLKPFDE+ELELVIGGIG IDI DWK HTRLK C+ +TPVV+WFW IVD YS EMRARLLQFVTGSSRVPLQGFK LQGSTG  GPR FTLH+    +++LPKA TCFNRLDLPAY S   M +KLTQAVEETCGFAVE
Sbjct:  544 EFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. L. salmonis genes
Match: EMLSAG00000011039 (supercontig:LSalAtl2s:LSalAtl2s744:109377:113126:-1 gene:EMLSAG00000011039 transcript:EMLSAT00000011039 description:"maker-LSalAtl2s744-snap-gene-1.41")

HSP 1 Score: 369.392 bits (947), Expect = 2.034e-113
Identity = 183/406 (45.07%), Postives = 260/406 (64.04%), Query Frame = 0

Query:  596 KYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAR--------------------EWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGI-LDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            +Y+R    K+   R   QS    S H +L V+RQ +FE+S+ Q++++   +L++RL I FRGE+GLDYGG AR                    EW  LLSHE+LNP Y LF+Y+  + Y+LQINP S +NPDHL YF F+GR I MA++HG  I  GFT PFYK +LNK + + DIE +DP  + SL W+ +N+I    L+  F V+ E  G ++  ELK GG +L+VTE+NK+EY+++   +R  RGIE+Q  +  +GFNE++P + L+ FDERELEL++ G+  ID++DW+ H+  +H   ++  ++WFW+ V     E R+RLLQFV G+ RVP+ GF  L GS GA   R     V + S   LP++HTCFNRLDLP Y+S  ++ +KL  A+EET GF +E
Sbjct:  475 QYERSFRWKLSQFRYLCQSNSLPS-HIKLSVTRQTLFEDSFHQLMRLPAFELRRRLYIIFRGEEGLDYGGXARXVDMLLLLPCSYVNLIYPLREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLHYFKFIGRFIAMALYHGKFIYSGFTMPFYKRMLNKKLIMKDIESIDPEYYNSLMWIKDNNIEECGLEMYFCVDFEVLGEIKPHELKPGGSELKVTEENKEEYIEIVCEWRMSRGIEEQTKSFLEGFNEVVPLEWLQYFDERELELMLCGMQVIDVDDWQRHSIYRHYTKNSKQIQWFWQFVRSMDHEKRSRLLQFVCGTCRVPVGGFAELMGSNGAQ--RFCVEKVGKDSW--LPRSHTCFNRLDLPPYKSYDQLVEKLNFAIEETEGFGLE 875          

HSP 2 Score: 71.633 bits (174), Expect = 7.487e-13
Identity = 40/111 (36.04%), Postives = 57/111 (51.35%), Query Frame = 0

Query:  298 PIGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSN---MSNDEVL-------------GPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHV 392
            P  LP G+E+R  E+G+IY+++  T  +TW  P + +  N +   G  +    M N   L             GP+  GWE+R    GR YFV+H +RTTQ+ DPR  G V
Sbjct:  311 PQPLPVGWEMRRDERGRIYYVDHNTRQTTWQRPNVDRLQNFASWQGERAQVLQMRNQRFLYPDQSRPSEEDPQGPLMDGWEKRVEPNGRVYFVNHKNRTTQWEDPRTQGQV 421          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. L. salmonis genes
Match: EMLSAG00000007514 (supercontig:LSalAtl2s:LSalAtl2s431:6204:41118:1 gene:EMLSAG00000007514 transcript:EMLSAT00000007514 description:"maker-LSalAtl2s431-snap-gene-0.13")

HSP 1 Score: 355.14 bits (910), Expect = 1.256e-108
Identity = 183/388 (47.16%), Postives = 250/388 (64.43%), Query Frame = 0

Query:  597 YKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKD----LKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSI-NPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLK-HCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFA 978
            Y RD   K + L+ +L+          +++ R+ + E+S+++I    P      LK +L I+F GE GLDYGG+AREW +LLS EM NPYYGLF+YS  D YTLQINP S + N DHLSYF F+GR  GMA   G  +DG F  PFYKM+L K + L D+E VD     SL W+ END    L+  F V+ E FG   ++ELK GGK++ VT +NK EY+   + +R+   I++Q      GFNEL+P   +K FDE ELEL++ GIG ID++DWK +T  K   + +  V++W+W +V  ++ EMR+RLLQFVTG+SRVP+ GFK L GS    GP+LFT+     +  + P+AHTCFNRLDLP YES  ++ D+L +A+E + GFA
Sbjct:  367 YNRDYKRKYEFLKSQLKKPSNVPNKFEIKIRRRYILEDSFQKIFMQFPSHKVDVLKTKLWIEFDGEIGLDYGGVAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGVCNEDHLSYFKFIGRXAGMAXXXGKLLDGFFIRPFYKMMLGKGMELSDMESVDTEXFNSLMWIRENDPQE-LELTFQVDEENFGTTVTRELKPGGKNIPVTNENKDEYIHQVIQWRFVSRIQKQMDNFLAGFNELVPLSFIKIFDEGELELLMCGIGSIDVKDWKQNTVYKGDYHPNHIVIQWYWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSN---GPQLFTIERWG-NPSSYPRAHTCFNRLDLPPYESYQQLRDRLIKAIEGSEGFA 749          

HSP 2 Score: 68.5514 bits (166), Expect = 5.810e-12
Identity = 39/113 (34.51%), Postives = 58/113 (51.33%), Query Frame = 0

Query:  292 PSTVISPIGLPDGFEIRHTEQG---------QIYFINVQTGVSTWHDPR------IPKDYNLSEVFGGSSNMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLS 389
            P   + P  LP G+E R    G         ++++IN     +TW DPR      +P   N+        N  +++ LGP+P GWE R  + GR +F+DH +RTTQ+ DPR+S
Sbjct:  251 PEGAVGPSALPAGWEERQDANGWTMQVAPNGRVFYINHADKETTWVDPRSGRPSALPSQANVP-------NRRHEDDLGPLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRIS 356          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. L. salmonis genes
Match: EMLSAG00000004991 (supercontig:LSalAtl2s:LSalAtl2s260:299952:311628:1 gene:EMLSAG00000004991 transcript:EMLSAT00000004991 description:"maker-LSalAtl2s260-augustus-gene-3.39")

HSP 1 Score: 287.73 bits (735), Expect = 7.134e-81
Identity = 142/384 (36.98%), Postives = 226/384 (58.85%), Query Frame = 0

Query:  600 DLVAKIKLLRQELQ--SQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGI-LDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            D   K +  R EL+   ++ +     + V R+ VFEES+R++ +  P++ K R  I F GE+G D GG+ REW  ++S E+ NP Y LF+ S  D  T  I   S  NP+HL YF FVGR+I  A++    ++  FT  FYK +L KP+   D+E  D   +  L +++ + ++ +  D  FS E + FG+ + ++L   G+++ VTE+ K +Y++L    +    I +Q  A  +GF  +IP++L+  F+E+ELEL++ G+  IDI+D K++T       ++  + WFW  +  + +  +A+ +QFVTGSS+VPLQGF  L+G  G    + F +H  + S + LP AHTCFN+LDLPAYE+  K+   L +A+ E C   +E
Sbjct: 2577 DFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGV---QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHE-CSEVME 2956          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. L. salmonis genes
Match: EMLSAG00000003493 (supercontig:LSalAtl2s:LSalAtl2s190:418567:426512:1 gene:EMLSAG00000003493 transcript:EMLSAT00000003493 description:"maker-LSalAtl2s190-snap-gene-4.16")

HSP 1 Score: 166.392 bits (420), Expect = 2.826e-42
Identity = 112/364 (30.77%), Postives = 186/364 (51.10%), Query Frame = 0

Query:  626 VSRQEVFEESYRQIVKMRPKDLKKRLMIKFRG-----EDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDS-SINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNK---PINLDDIEEVDPNLHTSLHWMLEND-ITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGI-GKIDIEDWKTHTRLK-HCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGF 977
            + R+ ++E++  ++      ++K ++ ++F+      E G+D GG+ RE+L  L     +P  G F+ ++D    L  NP    I  D L ++ F+GR++G A++    ++    + F   LL K    +++D ++ +DP L+ +L ++   D     L  +F++ +E  G    +ELK+ G  + VT  N+ EY+ L  NY+  + I  Q  A ++G +++I    LK F+ +EL+LVI G   +ID+ D + HT        D P V  FW +V+  +E  +  LL+FVT  SR PL GFK L           F +H        LP A TC N L LP +E +  + DKL  A+E   GF
Sbjct:  660 IRRKFIYEDALEKLSPEAEPNMKLKMRVEFKNIAGLREAGIDGGGLFREFLGELVKTSFDPNRGFFRLTKDQ--RLYPNPSVHKIYEDFLPHYFFIGRMLGKALYENMLVEFPLASFFLHKLLEKSSVKVDIDHLQSLDPELYRNLLYLKNYDGCVQDLGLDFTIVNEEVGTTSVEELKRNGSSIPVTNVNRTEYIHLIANYKLNKQIRLQSNAFRQGLSDVINLDWLKMFNYKELQLVISGAETEIDVSDLRNHTNYTGGYAVDHPTVVIFWNVVESMTETQKRHLLKFVTSCSRPPLLGFKELDPP--------FCVHNAGAEPHRLPTASTCMNLLKLPNFEEEKILKDKLLYAIESGSGF 1013          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. L. salmonis genes
Match: EMLSAG00000008825 (supercontig:LSalAtl2s:LSalAtl2s54:414186:494517:-1 gene:EMLSAG00000008825 transcript:EMLSAT00000008825 description:"maker-LSalAtl2s54-augustus-gene-5.10")

HSP 1 Score: 145.976 bits (367), Expect = 1.090e-35
Identity = 109/374 (29.14%), Postives = 181/374 (48.40%), Query Frame = 0

Query:  624 LEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGI-----AREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPIN-----LDDIEEVDPNLHTSLHWM--LENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERE-LELVIGGIGKIDIEDWKTHTRLKHCNTDT-PVVKWFWEIVD-GYSEEMRARLLQFVTGSSRVPLQGFKALQ----------------GSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDK 966
            + V R  + E+ YR + K+  + LK  + +KF    GLD  GI      +E+L      + +P   LF  + D+   L  +P S +  ++L  F FVG++IG AV+ G  ++  F   F   +L+K  +     LD++  +DP L+ +L ++   + ++T  LD  FS + +  G + + EL +GG+ + V + NK  Y+     +     I++Q  A  +GF  ++P   L  F   E L L+ G    +D++D + HT       D+  V+ W WEI+D  + ++ R+  L+FVT  S+ PL GF+ L+                G T  +  R F        +  LP A TCFN L LP Y+ +  + DK
Sbjct:  615 ITVHRSRIVEDGYRHLGKLSSRSLKGVIRVKFINTQGLDEAGIDQDGVFKEFLEETIKRVFDPGLNLFCSTSDN--RLYPSPTSHLTENYLQLFEFVGKMIGKAVYEGIVVEIPFAPFFISQILSKDHSAYYSYLDELPSMDPELYKNLTYIKYFDGNVTD-LDLTFSYDQDVLGRVVTHELIEGGRSVLVNDCNKISYIHHVAQFVLHSQIKEQVAAFTRGFRAILPIDWLNLFSPPEVLRLISGDNLPLDLKDLRRHTHYYGGFHDSHKVIVWLWEILDRDFDDKERSSFLKFVTSCSKPPLLGFENLEPAFSIRCVEVAEDEDDGDTVGSVLRGFLALKKRDPVNRLPTASTCFNLLKLPNYQKKATLRDK 985          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. L. salmonis genes
Match: EMLSAG00000009470 (supercontig:LSalAtl2s:LSalAtl2s60:50964:63500:-1 gene:EMLSAG00000009470 transcript:EMLSAT00000009470 description:"maker-LSalAtl2s60-augustus-gene-0.20")

HSP 1 Score: 120.553 bits (301), Expect = 8.497e-28
Identity = 87/302 (28.81%), Postives = 137/302 (45.36%), Query Frame = 0

Query:  712 FHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKP--INLDDIEEVDPNLHTSLHWM---------LEND-----------ITGILDSNFSVEHEAFGL----LQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHC------NTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLEN--------LPKAHTCFNRLDLPAYESQTKMHDKLTQAVEE 973
            F F+G+ I  AV     ID  F+ PFY+ LL +   + + D+  VDP + +++  +         LE D           I  +      +E            + +L+KGGKD+ V   N  +YV L  ++    GI  Q  AL+ GF  + P   L+ F   ELE +  G G+ +   W   T ++        N+++P +K+  E+++ Y+ + + + LQFVTG  R+P+ GFKAL             L +++ S +N        LP   TC N L LP Y S+  M +KL  A  E
Sbjct: 1444 FTFLGKFIAKAVLDNRMIDMPFSQPFYQWLLREESTLGISDLRNVDPTIASTISSLEGIARKKRKLEQDPQISPDEKLEQIKSLTMDGCPIEDLGLDFTLPGYPNIDLRKGGKDIPVNIDNLHQYVGLVSHWLLIEGISFQMEALRDGFESVFPISTLQMFYPEELEQIFCGTGQGNFNKWDYKTLVESTKPDHGFNSESPALKYLLEVLESYNIDEQRQFLQFVTGCPRLPVGGFKALNPP----------LTIVKKSFDNADADPDDYLPSVMTCVNYLKLPDYSSKKIMREKLRIAAME 1735          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. L. salmonis genes
Match: EMLSAG00000007808 (supercontig:LSalAtl2s:LSalAtl2s458:200727:208701:-1 gene:EMLSAG00000007808 transcript:EMLSAT00000007808 description:"maker-LSalAtl2s458-snap-gene-2.45")

HSP 1 Score: 110.538 bits (275), Expect = 4.807e-25
Identity = 93/326 (28.53%), Postives = 144/326 (44.17%), Query Frame = 0

Query:  651 LMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTS-LHWMLENDITGILDSNFS--VEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEE 973
            L I F GE G++YGG+ REW  LL  E+          S   I+       +  NP    Y  F                  F++ F   L    I     +   P  +TS + ++++N +   LD  F+  +  E   L++S+ LK  GK ++VT  NK EY+ L   YR    I+      + G +  + +  L  FDE E++L+  G   ID  D K H   +  N    +++WFW +++  SEE +   L+F+TGSSR+P  GFK L        P +FT+  +      LP +H C N L++  + S          A+ E
Sbjct:  385 LKISFSGEQGIEYGGLRREWYTLLWKEL----------SDTKIFITN-ERGTGANPMFRLYLPF-----------------SFSSSFLYQLKGINIGXRHFKVDYPEFYTSRIDYIVKNKVED-LDLYFTDEIYDEKGHLIESKNLKPSGKKIKVTNFNKFEYLHLLAKYRLSDRIKGPLTKFKTGLHYYVSETYLANFDESEMDLLFSGFSTIDFNDLKKHHNCQ--NVSHKIMEWFWLVLEHLSEEQKLLFLKFITGSSRLPPNGFKDL-------CP-VFTIRQLNEE-GKLPSSHVCANVLNIHPHSSFEAFESSFITAITE 670          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. L. salmonis genes
Match: EMLSAG00000002245 (supercontig:LSalAtl2s:LSalAtl2s1426:7958:26313:1 gene:EMLSAG00000002245 transcript:EMLSAT00000002245 description:"maker-LSalAtl2s1426-augustus-gene-0.12")

HSP 1 Score: 111.694 bits (278), Expect = 6.070e-25
Identity = 95/379 (25.07%), Postives = 173/379 (45.65%), Query Frame = 0

Query:  612 LQSQQPQSGHCRLE-VSRQEVFEESYRQIVKMRPKDLK---KRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQ---------YSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWML-----ENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVE 972
            L++  PQ    RL  +    +F +   Q+  + PK+L+   +   +K  GE   D GG+  + +  +  E+      L            S  D Y L  + +S ++  H   F F+G + G+A+     I        +K++L       D+E+VD +   SLH++      +N+   +L +++ +     G  +   L  G + + ++ +NK EYVK  +N R+ + +  Q LA++KG + L P  +LK    + +E ++ G     +   K   R +  +    V+ W WEI++ ++ +++   L FV+G SR+P   F   Q          F +  +E     LP A TCF +L LP Y S+  M ++L  A++
Sbjct: 2968 LRNYGPQVTVRRLFPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSG--REVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQR---------FQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIK 3331          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. L. salmonis genes
Match: EMLSAG00000000248 (supercontig:LSalAtl2s:LSalAtl2s1036:68871:78712:-1 gene:EMLSAG00000000248 transcript:EMLSAT00000000248 description:"maker-LSalAtl2s1036-augustus-gene-0.34")

HSP 1 Score: 92.4337 bits (228), Expect = 4.439e-19
Identity = 72/291 (24.74%), Postives = 129/291 (44.33%), Query Frame = 0

Query:  707 DHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWML---ENDITGI-----LDSNFSVE---HEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTP-----VVKWFWEIVDGYSEEMRARLLQFVTGSSRVPL--QGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVE-ETCGFA 978
            + L+ F  VGR++G+ +               K +L + I   D+   DP ++ SL  ++   EN  T       LD NFS++    E  G   S EL   G+++E    N   YV+ Y  YR  +  E+    ++ G  +++P   L      +  L++ G+G I+++   ++T     + ++        +W W++V+  S   +  L+ F TGS  +P    GF+ +   T           +      +LP A+TC +RL +P Y S+  +  KL  A++ +  GF 
Sbjct: 2723 ERLNAFRNVGRLMGLCLLQNELCPLFLNRHVIKYILGRTIRFHDLAFFDPVIYESLRQLVVDAENKETSAQLFSALDLNFSIDLSPEEGGG---SVELIPNGREVEXXANNVYCYVRRYSYYRMIKCQEKALSNIKIGLYDVLPAGALDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGESQDRLVNFKRWLWQMVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSIT-----------IRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLLAIKTKNFGFV 2999          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. SwissProt
Match: gi|218547424|sp|A9JRZ0.1|SMUF2_DANRE (RecName: Full=E3 ubiquitin-protein ligase SMURF2; AltName: Full=HECT-type E3 ubiquitin transferase SMURF2; AltName: Full=SMAD ubiquitination regulatory factor 2; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 2)

HSP 1 Score: 651.744 bits (1680), Expect = 0.000e+0
Identity = 337/634 (53.15%), Postives = 425/634 (67.03%), Query Frame = 0

Query:  357 MPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKIQTHFQQRSGPPPQTEGP-PPSAPVMESNPPGASPERPAPKTSEGAPRTAEAGANVDQTEPRSPTEESETTRPPLVPKTNGETSNNNNRSHSTSSSSSNQASSQPAT--SLANGHTPAVTPTSAAAEAAAPGASQP-------STPSATSASQSTVLSNTVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNPSHHDNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            +P GWE R T++GR  +++H++R+TQ+  P                 + P  +   P  P + +++ N P  +P   A   ++  PR  E    V     R+    +    PP +P+   + +    + +   + +       P     L+N +   + P     E       +         T   T    S  L   +N  P  S  +V   +SS   R  +    ++S  + GN  P   +   +PKYKRDLV K+K+LRQEL  QQPQ+GHCR+EVSR+E+FEESYRQ++KMRPKDL KRLM+KFRGE+GLDYGG+AREWL+LLSHEMLNPYYGLFQYSRDDIYTLQINPDS++NP+HLSYFHFVGRI+GMAVFHGH+IDGGFT PFYK LL KPI LDD+E VDP+LH SL W+L+NDITG+LD  F VEH A+G +   ELK  GK + VT+  KKEYV+LYVN+R+ RGIE QFLALQKGFNE+IPQ LLK FDE+ELEL++ G+GKIDI DWK++TRLKHC  D+ +VKWFW  V+ Y EE RARLLQFVTGSSRVPLQGFKALQ   GAAGPRLFT+H I+ S  NLPKAHTCFNR+D+P YES  K++DKL  A+EETCGFAVE
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRSTQWERP-----------------TRPASEYSSPGRPLSCLVDENTPIMTPNGAAGVPADD-PRVQER--RVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNVNCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNPSPNGSRAAVEAQSSS---RPGQLKEQAQSVVSPGNL-PEDPECLTVPKYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILDNDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTQDTKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDINDWKSNTRLKHCTPDSNIVKWFWRAVESYDEERRARLLQFVTGSSRVPLQGFKALQ---GAAGPRLFTIHQIDASTNNLPKAHTCFNRIDIPPYESYDKLYDKLLTAIEETCGFAVE 765          

HSP 2 Score: 312.383 bits (799), Expect = 5.544e-92
Identity = 187/379 (49.34%), Postives = 230/379 (60.69%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDL-VTDPHHAWPVKGQIVISLLSRDGQGTGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERPSRPALSDLSLNSIPG-----LQHQISSTSTASGGSASGALAPPTQRNGHAGEGRSGRGGSRRTNGTSAAAAGPVANPSTVISPIGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSNMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHV 392
            G  K+R+T+LCA NL K+D FRLPDPF K+ VD +G          Q H TDT R TLDPKWN HYDL +  +D ITISVWN KK    +G          GFLGCVRLLSN++ RLKDTGYQRLDL    P+ +  V+GQIV+SL SRD  G+G    VVD   +    N+LP+GWEERR  SGRI Y+NH+TR TQWERP+RPA    S  S PG     L  + +   T +G +   A  P  Q        R  R    R             + + + +P  LP+G+E R T+QGQ+YF++ QTGVSTWHDPR+P+D  LS V        N E LGP+P GWE R T+TGR YFVDH +RTTQFTDPRLS ++
Sbjct:   10 GPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSG----------QCHSTDTVRNTLDPKWNQHYDLYIGKSDSITISVWNHKKIHKKQGA---------GFLGCVRLLSNSINRLKDTGYQRLDLNKLGPNDSDTVRGQIVVSLQSRDRIGSGG--PVVDC--SRLFDNDLPDGWEERRTASGRIQYLNHITRSTQWERPTRPA----SEYSSPGRPLSCLVDENTPIMTPNGAAGVPADDPRVQE-------RRVRSQRHRN----------YMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRD--LSNV--------NCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANL 334          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. SwissProt
Match: gi|123896339|sp|Q2TAS2.1|SMUF2_XENLA (RecName: Full=E3 ubiquitin-protein ligase SMURF2; AltName: Full=HECT-type E3 ubiquitin transferase SMURF2; AltName: Full=SMAD ubiquitination regulatory factor 2; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 2)

HSP 1 Score: 650.588 bits (1677), Expect = 0.000e+0
Identity = 337/634 (53.15%), Postives = 420/634 (66.25%), Query Frame = 0

Query:  357 MPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKIQTHFQQRSGPPPQTEGPPPSAPVMESNPPGASPERPAPKTSEGAPRTAEAGANVDQTEPRSPTEESETTRPPLVPKTNGETSNNNNRSHSTSSSSSNQASSQPATSLANGHTPAVTPTSAAAEAAAPGASQPSTPSATSASQSTVLSNTVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNP------SHHDNEV----LPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            +P GWE R T++GR  +++H++RTTQ+  P                R      + G P S  V E+ P   +      +TS+  PR +E    V     R+    +    PP +P+   + +    + +   +           T ++  H P V            G   P      +A+      +  N       T  T P  S+++  +     +R N       P          +EV    +P+YKRDLV K+K LRQEL  QQPQ+GHCR+EVSR+E+FEESYRQ++KMRPKDL KRLMIKFRGE+GLDYGG+AREWL+LLSH+MLNPYYGLFQYSRDDIYTLQINPDS++NP+HLSYFHFVGRI+GMAVFHGH+IDGGFT PFYK LL KPI LDD+E VDP+LH SL W+LENDITG+LD  F VEH A+G L   ELK  GK + VTE  KKEYV+LYVN+R+ RGIE QFLALQKGFNE+IPQ LLK FDE+ELEL+I G+GKID+ DWK++TRLKHC TD+ +VKWFW+ V+ + EE RARLLQFVTGSSRVPLQGFKALQ   GAAGPRLFT+H I+ S  NLPKAHTCFNR+D+P YE+  K+++KL  A+EETCGFAVE
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWERP---------------TRPASEYSSPGRPLSCFVDENTPITGTNGASCGQTSD--PRISER--RVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQ----------TGVSTWHDPRVPRDLGNVNCEELGPLPPGWEIRNTATGRVYFVDHNN-----RTTQFTDPRLSANLHLV----LNRQNQQLKEQQPPQVVSLCQLPDEVECLTVPRYKRDLVHKLKSLRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHDMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGELIQHELKPSGKSIPVTEDTKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIICGLGKIDVSDWKSNTRLKHCTTDSNIVKWFWKAVESFDEERRARLLQFVTGSSRVPLQGFKALQ---GAAGPRLFTIHQIDASTNNLPKAHTCFNRIDIPPYETYEKLYEKLLTAIEETCGFAVE 751          

HSP 2 Score: 311.612 bits (797), Expect = 8.135e-92
Identity = 189/397 (47.61%), Postives = 234/397 (58.94%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLVT-DPHHAWPVKGQIVISLLSRDGQGTGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERPSRPALSDLSLNSIPG--LQHQISSTS--TASGGSASGALAPPTQRNGHAGEGRSGRGGSRRTNGTSAAAAGPVANPSTVISPIGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSNMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKIQTHFQQ-RSGPPPQT 410
            G  K+R+T+LCA NL K+D FRLPD F K+ VD +G          Q H TDT + TLDPKWN HYDL +  +D ITISVWN KK    +G          GFLGCVRLLSNA+ RLKDTGYQRLDL    P+    V+GQIV+SL SRD  G+G    VVD   +    N+LP+GWEERR  SGRI Y+NH+TR TQWERP+RPA    S  S PG  L   +   +  T + G++ G  + P          R     SR                + + +P  LP+G+E R T+QGQ+YF++ QTGVSTWHDPR+P+D  L  V        N E LGP+P GWE R T+TGR YFVDH +RTTQFTDPRLS ++   +    QQ +   PPQ 
Sbjct:   10 GPVKLRLTVLCAKNLVKKDFFRLPDSFAKVVVDGSG----------QCHSTDTVKNTLDPKWNQHYDLYIGKSDSITISVWNHKKIHKKQGA---------GFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGSGG--QVVDC--SRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPA----SEYSSPGRPLSCFVDENTPITGTNGASCGQTSDPRISERRVRSQRHRNYMSR----------------THLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRD--LGNV--------NCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQQLKEQQPPQV 353          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. SwissProt
Match: gi|218547425|sp|A2A5Z6.1|SMUF2_MOUSE (RecName: Full=E3 ubiquitin-protein ligase SMURF2; AltName: Full=HECT-type E3 ubiquitin transferase SMURF2; AltName: Full=SMAD ubiquitination regulatory factor 2; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 2)

HSP 1 Score: 647.892 bits (1670), Expect = 0.000e+0
Identity = 336/627 (53.59%), Postives = 416/627 (66.35%), Query Frame = 0

Query:  357 MPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKIQTHFQQRSGPPPQTEGPPPSAPVMESNPPGASPERPAPKTSEGAPRTAEAGANVDQTEPRSPTEESETTRPPLVPKTNGETSNNNNRSHSTSSSSSNQASSQPATSLANGHTPAVTPTSAAAEAAAPGASQPSTPSATSASQSTVLSNTVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNP-SHHDNEVL--PKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            +P GWE R T++GR  +++H++RTTQ+  P                R      + G P S  V E+ P   +       +S+  PR AE    V     R+    +    PP +P+   + +    + +   +           T ++  H P V    +       G   P      +A+      +  N       T  T P  S+++  +        +       P    D E L  P+YKRDLV K+K+LRQEL  QQPQ+GHCR+EVSR+E+FEESYRQ++KMRPKDL KRLMIKFRGE+GLDYGG+AREWL+LLSHEMLNPYYGLFQYSRDDIYTLQINPDS++NP+HLSYFHFVGRI+GMAVFHGH+IDGGFT PFYK LL K I LDD+E VDP+LH SL W+LENDITG+LD  F VEH A+G +   ELK  GK + VTE+NKKEYV+LYVN+R+ RGIE QFLALQKGFNE+IPQ LLK FDE+ELEL+I G+GKID+ DWK +TRLKHC  D+ VVKWFW+ V+ + EE RARLLQFVTGSSRVPLQGFKALQ   GAAGPRLFT+H I+    NLPKAHTCFNR+D+P YES  K+++KL  A+EETCGFAVE
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWERP---------------TRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSD--PRLAER--RVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQ----------TGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNN-----RTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQ---GAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE 748          

HSP 2 Score: 313.538 bits (802), Expect = 1.787e-92
Identity = 183/376 (48.67%), Postives = 230/376 (61.17%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLVT-DPHHAWPVKGQIVISLLSRDGQGTGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERPSRPALSDLSLNSIPG--LQHQISSTSTASGGSASGALAPPTQRNGHAGEGRSGRGGSRRTNGTSAAAAGPVANPSTVISPIGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSNMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHV 392
            G  K+R+T+LCA NL K+D FRLPDPF K+ VD +G          Q H TDT + TLDPKWN HYDL +  +D +TISVWN KK    +G          GFLGCVRLLSNA+ RLKDTGYQRLDL    P+    V+GQIV+SL SRD  GTG    VVD   +    N+LP+GWEERR  SGRI Y+NH+TR TQWERP+RPA    S  S PG  L   +   +  +G + +          GH+ + R      R     +  +       + + +P  LP+G+E R T+QGQ+YF++ QTGVSTWHDPR+P+D  LS +        N E LGP+P GWE R T+TGR YFVDH +RTTQFTDPRLS ++
Sbjct:   10 GPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSG----------QCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGA---------GFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGG--QVVDC--SRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPA----SEYSSPGRPLSCFVDENTPITGTNGATC--------GHSSDPRLAERRVRSQRHRNYMSR------THLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRD--LSNI--------NCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANL 334          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. SwissProt
Match: gi|17865624|sp|Q9HAU4.1|SMUF2_HUMAN (RecName: Full=E3 ubiquitin-protein ligase SMURF2; Short=hSMURF2; AltName: Full=HECT-type E3 ubiquitin transferase SMURF2; AltName: Full=SMAD ubiquitination regulatory factor 2; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 2)

HSP 1 Score: 647.506 bits (1669), Expect = 0.000e+0
Identity = 331/624 (53.04%), Postives = 415/624 (66.51%), Query Frame = 0

Query:  357 MPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKIQTHFQQRSGPPPQTEGPPPSAPVMESNPPGASPERPAPKTSEGAPRTAEAGANVDQTEPRSPTEESETTRPPLVPKTNGETSNNNNRSHSTSSSSSNQASSQPATSLANGHTPAVTPTSAAAEAAAPGASQPSTPSATSASQSTVLSNTVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNPSHHDNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            +P GWE R T++GR  +++H++RTTQ+  P                R      + G P S  V E+ P   +      ++S+  PR AE    V     R+    +    PP +P+   + +    + +   +           T ++  H P V    +       G   P      +A+      +  N   TT  T      +   + N +     +      +  P   +   +P+YKRDLV K+K+LRQEL  QQPQ+GHCR+EVSR+E+FEESYRQ++KMRPKDL KRLMIKFRGE+GLDYGG+AREWL+LLSHEMLNPYYGLFQYSRDDIYTLQINPDS++NP+HLSYFHFVGRI+GMAVFHGH+IDGGFT PFYK LL K I LDD+E VDP+LH SL W+LENDITG+LD  F VEH A+G +   ELK  GK + V E+NKKEYV+LYVN+R+ RGIE QFLALQKGFNE+IPQ LLK FDE+ELEL+I G+GKID+ DWK +TRLKHC  D+ +VKWFW+ V+ + EE RARLLQFVTGSSRVPLQGFKALQ   GAAGPRLFT+H I+    NLPKAHTCFNR+D+P YES  K+++KL  A+EETCGFAVE
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWERP---------------TRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSD--PRLAER--RVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQ----------TGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNR--TTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQ---GAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE 748          

HSP 2 Score: 313.923 bits (803), Expect = 1.316e-92
Identity = 184/376 (48.94%), Postives = 228/376 (60.64%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLVT-DPHHAWPVKGQIVISLLSRDGQGTGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERPSRPALSDLSLNSIPG--LQHQISSTSTASGGSASGALAPPTQRNGHAGEGRSGRGGSRRTNGTSAAAAGPVANPSTVISPIGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSNMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHV 392
            G  K+R+T+LCA NL K+D FRLPDPF K+ VD +G          Q H TDT + TLDPKWN HYDL +  +D +TISVWN KK    +G          GFLGCVRLLSNA+ RLKDTGYQRLDL    P+    V+GQIV+SL SRD  GTG    VVD   +    N+LP+GWEERR  SGRI Y+NH+TR TQWERP+RPA    S  S PG  L   +   +  SG + +             G+    R   RR             + + + +P  LP+G+E R T+QGQ+YF++ QTGVSTWHDPR+P+D  LS +        N E LGP+P GWE R T+TGR YFVDH +RTTQFTDPRLS ++
Sbjct:   10 GPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSG----------QCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGA---------GFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGG--QVVDC--SRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPA----SEYSSPGRPLSCFVDENTPISGTNGAT-----------CGQSSDPRLAERRVRSQRHRN---YMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRD--LSNI--------NCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANL 334          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. SwissProt
Match: gi|73919319|sp|Q9V853.3|SMUF1_DROME (RecName: Full=E3 ubiquitin-protein ligase Smurf1; AltName: Full=HECT-type E3 ubiquitin transferase Smurf1; AltName: Full=Lethal with a checkpoint kinase protein; AltName: Full=SMAD ubiquitination regulatory factor 1 homolog; Short=DSmurf)

HSP 1 Score: 634.024 bits (1634), Expect = 0.000e+0
Identity = 290/390 (74.36%), Postives = 336/390 (86.15%), Query Frame = 0

Query:  592 EVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVI-EVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            ++LPKY+RDLV K++ LR ELQ+ QPQSGHCRLEVSR E+FEESYR I+KMR KD++KRLM+KF+GE+GLDYGG+AREWLHLLS EMLNP YGLFQYSRDD YTLQINPDS +NPDHLSYFHFVGR +G+AVFHGH +DGGFTTPFYK LLNKPI L DIE VDP+LH SL WMLE++I+GI++S FSVE+ +FG L   ELK GG  + VTE+NK+EYVKLYVNYR+ RGIEQQFLALQKGF ELIP  LL+PFDERELELVIGGI  ID+ DW+ +TRLKHC  +T  V WFW++V+ YS EMRARLLQFVTGSSRVPLQGF+ALQGSTGA GPRLFT+H+  +V  +NLPKAHTCFNR+DLP YE+   + DKLTQAVEETCGFAVE
Sbjct:  672 DLLPKYRRDLVGKLRALRTELQTMQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKHCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAVE 1061          

HSP 2 Score: 201.445 bits (511), Expect = 8.137e-52
Identity = 106/227 (46.70%), Postives = 141/227 (62.11%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDL-VTDPHHAWPVKGQIVISLLSRDGQGTGSLNAVVDSLGNLRCANE-------LPEGWEERRATSGRIYYVNHVTRCTQWERPSRPALSDLSLNSIPGLQH 238
            G  K+R+TILCA NLA++DLFRLPDPF K+ VD  G          Q++ T+  + +LDPKWNAHYDL L   D ITI+VWN++K   G G           FLGCVR+ +  ++ LK  G+QRLDL    P     V+GQI+ISLLS+DG  +G+  A+V   G++R  +E       LPEGWEERR  +GR+YYVNH T+ TQW+RP +P +   S  + P  +H
Sbjct:   11 GTHKVRITILCARNLARKDLFRLPDPFAKVQVDGTG----------QVYSTEISKSSLDPKWNAHYDLFLGIGDAITITVWNQRKIHKGSG-----------FLGCVRIPAFNIQSLKGAGFQRLDLGKLSPDDDELVRGQIIISLLSKDGPSSGNPLAIVGPSGDVRGPSEDDSSEDSLPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVVGSSHATSPQQRH 216          

HSP 3 Score: 123.635 bits (309), Expect = 2.079e-27
Identity = 54/99 (54.55%), Postives = 76/99 (76.77%), Query Frame = 0

Query:  299 IGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSNMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKIQ 397
            + LP G+E+R T+QGQ+YF ++ TGVSTWHDPRIP+D++   +          + +GP+P+GWE+R+T++GR YFVDH +RTTQFTDPRLSG +LQ I+
Sbjct:  513 LDLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIPRDFDTQHL--------TLDAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRLSGSILQMIR 603          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. SwissProt
Match: gi|85681896|sp|Q9CUN6.2|SMUF1_MOUSE (RecName: Full=E3 ubiquitin-protein ligase SMURF1; AltName: Full=HECT-type E3 ubiquitin transferase SMURF1; AltName: Full=SMAD ubiquitination regulatory factor 1; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 1)

HSP 1 Score: 633.254 bits (1632), Expect = 0.000e+0
Identity = 289/393 (73.54%), Postives = 344/393 (87.53%), Query Frame = 0

Query:  590 DNEVLP--KYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            ++E LP  +Y+RDLV K+K+LR EL  QQPQ+GHCR+EVSR+E+FEESYRQI+KMRPKDLKKRLM+KFRGE+GLDYGG+AREWL+LL HEMLNPYYGLFQYS D+IYTLQINPDSSINPDHLSYFHFVGRI+G+AVFHGH+I+GGFT PFYK LL KPI L D+E VDP LH SL W+LENDIT +LD  F VEH AFG +   ELK  G+++ VTE+NKKEYV+LYVN+R+ RGIE QFLALQKGFNELIPQ LLKPFD++ELEL+IGG+ KID+ DWK++TRLKHC  D+ +V+WFW+ V+ + EE RARLLQFVTGS+RVPLQGFKALQGSTGAAGPRLFT+H+I+ + +NLPKAHTCFNR+D+P YES  K+++KL  AVEETCGFAVE
Sbjct:  339 EDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE 731          

HSP 2 Score: 165.622 bits (418), Expect = 4.899e-41
Identity = 95/174 (54.60%), Postives = 111/174 (63.79%), Query Frame = 0

Query:   13 RSSSSSVGVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLVT-DPHHAWPVKGQIVISLLSRDGQGTGSLNAVVDSLGNL 185
            R + SS+   KIR+T+LCA NLAK+D FRLPDPF K+ VD +G          Q H TDT + TLDPKWN HYDL +  TD ITISVWN KK    +G          GFLGCVRLLSNA+ RLKDTGYQRLDL   +P     V+GQIV+SL +RD  G G   +VVD  G L
Sbjct:    7 RRNGSSI---KIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSG----------QCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGA---------GFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGG--SVVDCRGLL 156          

HSP 3 Score: 112.079 bits (279), Expect = 5.565e-24
Identity = 58/106 (54.72%), Postives = 73/106 (68.87%), Query Frame = 0

Query:  301 LPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSNMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKIQTHFQQRSGP 406
            LP+G+E R T QGQ+YF++ QTGVSTWHDPRIP+D N         +++ DE LGP+P GWE R T +GR YFVDH +RTTQFTDPRL  H +   Q   ++ S P
Sbjct:  236 LPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLN---------SVNCDE-LGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRL--HHIMNHQCQLKEPSQP 329          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. SwissProt
Match: gi|17865625|sp|Q9HCE7.2|SMUF1_HUMAN (RecName: Full=E3 ubiquitin-protein ligase SMURF1; Short=hSMURF1; AltName: Full=HECT-type E3 ubiquitin transferase SMURF1; AltName: Full=SMAD ubiquitination regulatory factor 1; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 1)

HSP 1 Score: 632.869 bits (1631), Expect = 0.000e+0
Identity = 288/393 (73.28%), Postives = 343/393 (87.28%), Query Frame = 0

Query:  590 DNEVLP--KYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            ++E LP  +Y+RDLV K+K+LR EL  QQPQ+GHCR+EVSR+E+FEESYRQI+KMRPKDLKKRLM+KFRGE+GLDYGG+AREWL+LL HEMLNPYYGLFQYS D+IY LQINPDSSINPDHLSYFHFVGRI+G+AVFHGH+I+GGFT PFYK LL KPI L D+E VDP LH SL W+LENDIT +LD  F VEH AFG +   ELK  G+++ VTE+NKKEYV+LYVN+R+ RGIE QFLALQKGFNELIPQ LLKPFD++ELEL+IGG+ KID+ DWK++TRLKHC  D+ +V+WFW+ V+ + EE RARLLQFVTGS+RVPLQGFKALQGSTGAAGPRLFT+H+I+ + +NLPKAHTCFNR+D+P YES  K+++KL  AVEETCGFAVE
Sbjct:  365 EDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE 757          

HSP 2 Score: 252.292 bits (643), Expect = 2.836e-70
Identity = 177/413 (42.86%), Postives = 214/413 (51.82%), Query Frame = 0

Query:   13 RSSSSSVGVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLVT-DPHHAWPVKGQIVISLLSRDGQGTGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERPSRPALSDLSLNSIPGLQHQISSTSTASGGSASGALAPPTQRNGHAGEGRSGRGGSRRTNGTSAAAAGPVANPSTVISPIGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPK--------DYNLSEVF---GGSS-----NMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKIQTHFQQRSGPPP 408
            R + SS+   KIR+T+LCA NLAK+D FRLPDPF K+ VD +G          Q H TDT + TLDPKWN HYDL +  TD ITISVWN KK    +G          GFLGCVRLLSNA+ RLKDTGYQRLDL   +P     V+GQIV+SL +RD  GTG   +VVD  G L                 G +Y         +   P RP LS       P       ST  A+GG     +  P+Q        +  +    R      +   P   P    SP  LP+G+E R T QGQ+YF++ QTGVSTWHDPRIP         D      F   G +S     N  N + LGP+P GWE R T +GR YFVDH +RTTQFTDPRL  H +   Q   ++ S P P
Sbjct:    7 RRNGSSI---KIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSG----------QCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGA---------GFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGG--SVVDCRGLLE--------------NEGTVY---------EDSGPGRP-LSCFMEEPAP----YTDSTGAAAGGGNCRFVESPSQ-------DQRLQAQRLRNPDVRGSLQTPQNRPHGHQSP-ELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPSGTIPGGDAAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRL--HHIMNHQCQLKEPSQPLP 357          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. SwissProt
Match: gi|17865628|sp|Q9PUN2.1|SMUF1_XENLA (RecName: Full=E3 ubiquitin-protein ligase SMURF1; Short=xSMURF1; AltName: Full=HECT-type E3 ubiquitin transferase SMURF1; AltName: Full=SMAD ubiquitination regulatory factor 1; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 1)

HSP 1 Score: 629.402 bits (1622), Expect = 0.000e+0
Identity = 286/385 (74.29%), Postives = 337/385 (87.53%), Query Frame = 0

Query:  596 KYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            +Y+RDLV K+K+LR EL   QPQ+GHCR+EVSR+E+FEESYRQI+KMRPKDLKKRLM+KFRGE+GLDYGG+AREWL+LL HEMLNPYYGLFQYS D+IYTLQINPDSSINPDHLSYFHFVGRI+G+AVFHGH+I+GGFT PFYK LL KPI L D+E VDP LH SL W+LENDIT +LD  F VEH AFG L   ELK  GK+L+VTE+NKKEYV+LYVN+R+ RGIE QFLALQKGFNELIPQ LLKPF+++ELEL+IGG+ KIDI DWK +TRLKHC  ++ +V+WFW+ V+ + EE RARLLQFVTGS+RVPLQGFKALQGSTGAAGPRLFT+H+I+ + +NLPKAHTCFNR+D+P YES  K+++KL  AVEET GFAVE
Sbjct:  347 RYERDLVQKLKVLRHELSLLQPQAGHCRVEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSVLDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETSGFAVE 731          

HSP 2 Score: 164.466 bits (415), Expect = 1.260e-40
Identity = 93/174 (53.45%), Postives = 110/174 (63.22%), Query Frame = 0

Query:   13 RSSSSSVGVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLVT-DPHHAWPVKGQIVISLLSRDGQGTGSLNAVVDSLGNL 185
            R   SS+   ++R+T+LCA NLAKRD FRLPDPF K+ VD +G          Q H TDT + TLDPKWN HYDL +   D ITIS+WN KK    +G          GFLGCVRLLSNA+ RLKDTGYQRLDL   +P     V+GQIV+SL +RD  GT  L +VVD  G L
Sbjct:    7 RRGGSSI---RVRLTVLCAKNLAKRDFFRLPDPFAKIVVDGSG----------QCHSTDTVKNTLDPKWNQHYDLYVGKMDSITISIWNHKKIHKKQGA---------GFLGCVRLLSNAISRLKDTGYQRLDLCKLNPTDNDAVRGQIVVSLQTRDRIGT--LGSVVDCRGLL 156          

HSP 3 Score: 108.997 bits (271), Expect = 5.446e-23
Identity = 57/112 (50.89%), Postives = 72/112 (64.29%), Query Frame = 0

Query:  301 LPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSNMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKIQTHFQQRSGPPPQTEG 412
            LP+G+E R T QGQ+YF++ QTGVSTWHDPRIP+D N       S N  +   LG +P GWE R T +GR YFVDH +RTTQFTDPRL  H++       +     P Q++G
Sbjct:  235 LPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLN-------SVNCDD---LGSLPAGWEVRTTVSGRIYFVDHNNRTTQFTDPRLH-HIINHQSQLKEPNHAIPVQSDG 335          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. SwissProt
Match: gi|3915818|sp|Q92462.2|PUB1_SCHPO (RecName: Full=E3 ubiquitin-protein ligase pub1; AltName: Full=HECT-type E3 ubiquitin transferase pub1)

HSP 1 Score: 446.432 bits (1147), Expect = 2.480e-142
Identity = 244/527 (46.30%), Postives = 326/527 (61.86%), Query Frame = 0

Query:  485 HSTSSSSSNQASSQPATSLANGHTPAVTPTSAAAEAAA----PGASQPSTPSA--TSASQSTVLSNTVNAPPTTSVTSVTTPTSSS-------------SIRNIRATSYSRSNSTNGNNN---------PSHHDNEVLPKYKRDLVAK-IKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLE--NLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            HS++SS++     QP++S A   T A   TS A  A +    PG  Q  TP         +T  +  V+      + S   P +++             S   +R T+ +R    + N           PS  D  V P+YKRD   K I  L Q   +  P  G C ++V R  +FE+SY +I++    DLKKRLMIKF GEDGLDYGG++RE+  LLSHEM NP+Y LF+YS  D YTLQINP S INP+HL+YF F+GR+IG+A+FH   +D  F   FYKM+L K + L D+E +D   + SL W+L+NDITG+LD  FSVE   FG + + +LK  G+++EVTE+NK+EYV L   +R  + IE+QF A  +GF+ELIPQ+L+  FDERELEL+IGGI +ID+EDWK HT  +  + +  ++KWFWE++D +S E ++RLLQF TG+SR+P+ GFK LQGS    GPR FT   IE + E   LPKAHTCFNRLDLP Y S+  +  KL+ AVEET GF  E
Sbjct:  250 HSSASSANVTEGVQPSSSNAARRTEASVLTSNATTAGSGELPPGWEQRYTPEGRPYFVDHNTRTTTWVDPRRQQYIRSYGGPNNATIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNV-PQYKRDFRRKLIYFLSQP--ALHPLPGQCHIKVRRNHIFEDSYAEIMRQSATDLKKRLMIKFDGEDGLDYGGLSREYFFLLSHEMFNPFYCLFEYSSVDNYTLQINPHSGINPEHLNYFKFIGRVIGLAIFHRRFVDAFFVVSFYKMILQKKVTLQDMESMDAEYYRSLVWILDNDITGVLDLTFSVEDNCFGEVVTIDLKPNGRNIEVTEENKREYVDLVTVWRIQKRIEEQFNAFHEGFSELIPQELINVFDERELELLIGGISEIDMEDWKKHTDYRSYSENDQIIKWFWELMDEWSNEKKSRLLQFTTGTSRIPVNGFKDLQGSD---GPRKFT---IEKAGEPNKLPKAHTCFNRLDLPPYTSKKDLDHKLSIAVEETIGFGQE 767          

HSP 2 Score: 105.531 bits (262), Expect = 5.763e-22
Identity = 71/210 (33.81%), Postives = 102/210 (48.57%), Query Frame = 0

Query:  191 LPEGWEERRATSGRIYYVNHVTRCTQWERPSRPALSDLSLNSIPGLQHQISSTSTASGGSASGALAPPTQRNGHAGEGRSGRGGSRRTNGTSAAAAGPVANPSTVISPIGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSNMSNDEV----LGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKI 396
            LP GWE R    GR YYV+H TR T W RP+                      S+ +G +A+   +  +  N   G   S    +RRT  +   +    A          LP G+E R+T +G+ YF++  T  +TW DPR  + Y  S  +GG +N +  +     LGP+P+GWE R T+T R YFVDH ++TT + DPRL   + Q +
Sbjct:  207 LPPGWERRTDNLGRTYYVDHNTRSTTWIRPN---------------------LSSVAGAAAAELHSSASSANVTEGVQPSSSNAARRTEASVLTSNATTAGSGE------LPPGWEQRYTPEGRPYFVDHNTRTTTWVDPR-RQQYIRS--YGGPNNATIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNV 386          

HSP 3 Score: 58.5362 bits (140), Expect = 2.040e-7
Identity = 32/86 (37.21%), Postives = 45/86 (52.33%), Query Frame = 0

Query:   16 SSSVGVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWN 101
            S+S    +IRVTI+ A  L KRD+FR PDPF  L+VD             Q H T   + TL+P WN  +++ +     I I V++
Sbjct:    2 SNSAQSRRIRVTIVAADGLYKRDVFRFPDPFAVLTVDGE-----------QTHTTTAIKKTLNPYWNETFEVNVTDNSTIAIQVFD 76          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. SwissProt
Match: gi|1008808725|sp|G0S9J5.2|RSP5_CHATD (RecName: Full=E3 ubiquitin-protein ligase RSP5; AltName: Full=HECT-type E3 ubiquitin transferase RSP5)

HSP 1 Score: 441.81 bits (1135), Expect = 5.571e-140
Identity = 208/396 (52.53%), Postives = 281/396 (70.96%), Query Frame = 0

Query:  586 PSHHDNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDIT-GILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            PS  D  V P+YKRD   K+   R +  + +  SG C ++V R  +FE+++ +I +    DLKKRLMIKF GEDGLDYGG++RE+  LLSHEM NP+Y LF+YS  D YTLQINP S INP+HL+YF F+GR++G+A+FH   +D  F T FYKM+L KP+ L D+E VD + H SL WML+NDI+ GI+++ FS E E FG++  ++LK  G+++EVT +NK+EYV+L V +R  + +E+QF A ++GFNELIPQ L+  FDERELEL+IGGI +ID++DWK HT  +       V+++FW+ V  +  E ++RLLQF TG+SR+P+ GFK LQGS    GPR FT+      + NLPKAHTCFNRLDLP Y++  ++  KLT AVEET GF  E
Sbjct:  431 PSSLDQNV-PQYKRDFRRKLIYFRSQ-PAMRILSGQCHIKVRRSHIFEDAFAEISRQSATDLKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCLFEYSAHDNYTLQINPHSGINPEHLNYFKFIGRVVGLAIFHRRFLDAFFITAFYKMILGKPVTLADMEGVDADFHRSLQWMLDNDISGGIIEATFSTEDERFGVITVEDLKPNGRNIEVTNENKREYVELMVKWRIQKRVEEQFKAFKEGFNELIPQDLINVFDERELELLIGGIAEIDVDDWKKHTDYRGYTESDEVIQFFWQTVRSWDSEQKSRLLQFTTGTSRIPVNGFKDLQGSD---GPRRFTIERAG-DINNLPKAHTCFNRLDLPPYKTLEQLQQKLTMAVEETMGFGQE 820          

HSP 2 Score: 93.9745 bits (232), Expect = 2.934e-18
Identity = 75/232 (32.33%), Postives = 104/232 (44.83%), Query Frame = 0

Query:  175 LNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERPSRPALSDLSLNSIPGLQHQISSTSTASGGSASGALAPPTQRNGHAGEGRSGRGG---SRRTNGTSAAAAGPVANPSTVISPIGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSNMSNDEV-------LGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKI 396
            L+   D+LG       LP GWE R    GR YYV+H TR T W RP+    SD                       A+        +N    E R+G       ++     A A   V   +T      LP G+E+R T +G+ YF++  T  +TW DPR  +      ++GG  N  N  +       LGP+P+GWE R T+T R YFVDH ++TT + DPRL   + Q +
Sbjct:  239 LSPFEDALG------RLPPGWERREDHLGRTYYVDHNTRTTSWNRPTGTGQSD---------------------AEATQQAQRQQHQNRSLPEDRTGANSPTLQQQQAVAQAQATALVHTGATTAGTGELPPGWEMRWTPEGRPYFVDHNTRTTTWVDPRRQQ---YIRMYGG--NNPNGIIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNV 438          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. nr
Match: gi|1325267423|ref|XP_023341202.1| (E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267425|ref|XP_023341211.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267427|ref|XP_023341220.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267429|ref|XP_023341227.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267431|ref|XP_023341234.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267433|ref|XP_023341242.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267435|ref|XP_023341251.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267437|ref|XP_023341257.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267439|ref|XP_023341263.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267441|ref|XP_023341270.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267443|ref|XP_023341277.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267445|ref|XP_023341285.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267447|ref|XP_023341293.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267449|ref|XP_023341299.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267451|ref|XP_023341304.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267453|ref|XP_023341312.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267455|ref|XP_023341320.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267457|ref|XP_023341327.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267459|ref|XP_023341335.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267461|ref|XP_023341343.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267463|ref|XP_023341351.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267465|ref|XP_023341362.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267467|ref|XP_023341368.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267469|ref|XP_023341374.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267471|ref|XP_023341384.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267473|ref|XP_023341391.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267475|ref|XP_023341399.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267477|ref|XP_023341409.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267479|ref|XP_023341415.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267481|ref|XP_023341422.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267483|ref|XP_023341429.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267485|ref|XP_023341438.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267487|ref|XP_023341445.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267489|ref|XP_023341451.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267491|ref|XP_023341457.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267493|ref|XP_023341465.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267495|ref|XP_023341472.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267497|ref|XP_023341479.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267499|ref|XP_023341485.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267501|ref|XP_023341491.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis] >gi|1325267503|ref|XP_023341499.1| E3 ubiquitin-protein ligase SMURF2-like [Eurytemora affinis])

HSP 1 Score: 1031.17 bits (2665), Expect = 0.000e+0
Identity = 561/1003 (55.93%), Postives = 662/1003 (66.00%), Query Frame = 0

Query:   16 SSSVGVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLVTDPHHAWPVKGQIVISLLSRDGQGTGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERPSRPALSD---LSLNSIPGLQHQISSTSTASGGSASGALAPPTQRNGHAGEGRSGRGGSRRTNGTSAAAAGPVANPSTVI--SPIGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSNMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKIQTHFQQRSGPPPQTEGPPPSAPVMESNPPGASPERPAPKTSEGAPRTAEAGANVDQTEPRSP-----TEESETTRPPLVPKTNGETSNNNNRSHSTSSSSSNQASSQPATSLANGHTPAVTPTSAAAEAAAPGASQPSTPSATSASQSTVLSNTVNA------PPTTSVTSVTTPTSSSSIR-------------------NIRATS-YSRSNSTNGNNNPS--HHDNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
            S+  G  KIRVTILCA NLAKRDLFRLPDPF ++SVD +G          Q H TD  R TLDPKWN HYDL +  +D ITISVWN+KK +   G          GFLGCVRLL N++ RLKDTGYQRLDLV+D ++  PVKGQIV+SLLSRDG GTGSLNAVVD LGNL C  +LPEGWEERR TSGR+YYVNH  R TQWERP+RPA+ +   LS    P      S+  + +G   S     P        EG         T G +   A      S  +   P  LP GFE+R T+QGQIYF +V +G+STWHDPRIP+D    E             LG +P GWE+RET +GR YFV+H +RTTQFTDPRL+  +   I+   + RS   P+TE              G S E        G  R     +  D    R       TE   +   PL P+    +S+  + + ++S+     +   P T +    +PA +             S+P    A        +S    A      PP  +VT+V  P    ++                     +R +S Y  S   N +++P     + E LPKYKRDLVAK+K+LR EL S Q QSGHCRLEVSRQEVFE+SYRQ+VKMRPKD++KRLM+KF+ E+GLDYGG+AREWL+LLSHEMLNPYYGLFQYSRDDIYTLQIN DSSINPDHLSYFHFVGRIIG+A+FHGH+IDGGFT PFYKMLLNK INL DIE VDPNL+TSL WMLENDIT I+D+ FSVEH++FG L+  ELKK GK++ V E NKKEYVKLYVNYR+  GIEQQFLALQKGFNELIPQ LLK FDERELELVIGG+GKID+EDW+++TRLKHC  DT  V WFW+IV+GYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLH+++V L NLPKAHTCFNRLD+P YE+  KM DKLTQAVEETCGFAVE
Sbjct:    5 SNRNGSVKIRVTILCARNLAKRDLFRLPDPFARISVDGSG----------QTHTTDISRNTLDPKWNQHYDLYIGKSDAITISVWNDKKVQKKSGA---------GFLGCVRLLGNSINRLKDTGYQRLDLVSDNNNPLPVKGQIVVSLLSRDGHGTGSLNAVVDPLGNLSCPADLPEGWEERRTTSGRVYYVNHYLRTTQWERPTRPAVENSNPLSNPRFPTDTSLPSAAQSFTGEPTSSTPRRPEPDPVDLVEGERPPPCDPLTPGNNKVEANKQRFMSRTVLHQPQDLPAGFEMRTTQQGQIYFHHVPSGISTWHDPRIPRDAVPPEGVE----------LGTLPPGWEKRETPSGRGYFVNHHNRTTQFTDPRLNTSL---IKAMLKSRSESKPRTEN-------------GNSSEI-------GENRNENTNSGTDIVNARGENGNGRTENQNSVPAPLPPRL---SSSGPSSTPASSNGDVFHSVISPPTPV--NMSPAKSSRPLPPLPTETPTSEPEPAPADEQVDGLAMSCLAMALEPPARPPNPAVTNVRAPHILPNLAILQRDSSSPRSDSLQDPGPGVRTSSGYVPSPQNNKSSSPKILFENEECLPKYKRDLVAKMKVLRSELSSLQAQSGHCRLEVSRQEVFEDSYRQVVKMRPKDMRKRLMVKFKMEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINADSSINPDHLSYFHFVGRIIGIAMFHGHYIDGGFTMPFYKMLLNKSINLMDIESVDPNLYTSLKWMLENDITDIIDTTFSVEHDSFGCLKVHELKKNGKNIPVNESNKKEYVKLYVNYRFMHGIEQQFLALQKGFNELIPQCLLKSFDERELELVIGGLGKIDLEDWRSNTRLKHCQPDTIQVGWFWKIVEGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHLVDVDLNNLPKAHTCFNRLDMPPYETFEKMSDKLTQAVEETCGFAVE 950          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. nr
Match: gi|954547606|ref|XP_014616349.1| (PREDICTED: E3 ubiquitin-protein ligase SMURF1 isoform X1 [Polistes canadensis] >gi|954547608|ref|XP_014616350.1| PREDICTED: E3 ubiquitin-protein ligase SMURF1 isoform X1 [Polistes canadensis])

HSP 1 Score: 885.56 bits (2287), Expect = 0.000e+0
Identity = 506/996 (50.80%), Postives = 611/996 (61.35%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLV-TDPHHAWPVKGQIVISLLSRDGQG-----TGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERPS-RPALSDLSLNSIPGLQHQISSTSTASGGSASGALAPPTQRNGHAGEGRS-----GRGGSRRTNGTSAAAAGPVANPSTVISPIGLPDGFEIRHT-------EQGQIYFINVQTGVSTWHDPRIPKDYN----------LSEVFGGSSNMSNDEVLGPM--PTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHV-LQKIQTHFQQRSGPPPQTEGPPPSAPVMESNPPGASPERPAPKTSEGAPRTAEAGANVDQTEPRSPTEESETTRPPLVPKTNGETSNNNNRSHSTSSSSSNQASSQPATSLANGHTPAV--TPTSAAAEAAAPGASQPSTPSATSASQSTVLSNTVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNPSHHDNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLP-AYESQTKMHDKLTQAVEETCGFAVE 980
            G  KIR+TILCA NLA++DLFRLPDPF K++VD +G          Q H TDT + TL PKWN HYDL +   D ITISVWN +K    +G          GFLGCVR+LSN ++RLKDTGYQRLDL          VKGQIV+SLLSRDG       T   NAVVD LG+L C  +LP+GWEERR  SGR+YY+NH TR TQW RP+ RP+      N+I     +      +   S SG+ +PP  R       +S     G G S RT    ++A     N  T            +R+T       ++  +   + Q G     + RI +  N           +      S    +E L PM  P G+E R+T  G+ YF    + T+ + DPR+   +   ++ T             GP PS   M     G               R      N   T+   P   S+              SN  NR  +  +S++NQ S  P  S A  + P    TP S+     +    +P   + ++ +  T+ ++T   PPT +V+ +      S                           E+LPKYKRDLVAK+K LR EL + QPQSGHCRLEVSR E+FEESYR I+KMRPKD++KRLM+KFRGE+GLDYGG+AREWL+LLSHEMLNP YGLFQYSRDD YTLQINPDS INP+HLSYFHF GRIIG+AVFHGHHIDGGFTTPFYKMLLNK I L DIE VDP LH SL WMLEN I G+LD+ FSVEH +FG+L++ ELK GGKD++VTE+NKKEYV+LYVNYR+ RGIEQQFLALQKGF+ELIP  LL+PFDERELELVIGG+G IDI DWK HTRLKHC  DTPVVKWFW+IV+ Y EEMRARLLQFVTGSSRVPLQGFKALQGSTGA+GPRLFT+H ++   ENLPKAHTCFNR+D+P +Y    KM DKLTQAVEETCGFAVE
Sbjct:   11 GAMKIRLTILCARNLARKDLFRLPDPFAKITVDGSG----------QCHSTDTCKATLAPKWNQHYDLYIGKNDGITISVWNHRKIHKKQG---------GGFLGCVRILSNTIQRLKDTGYQRLDLCKAHSEDTDVVKGQIVVSLLSRDGHNGAVSNTVGHNAVVDVLGDLSCPTDLPDGWEERRTQSGRLYYINHYTRTTQWIRPNNRPS------NAIIPTTPEPPPLPNSEPTSPSGSGSPPNVRTDSPTVLQSTPEWNGEGTSSRTPSRDSSAQNTPRNTPTQQQNRSQQSQQHMRNTPPSSAIRDRRPVRSSDEQNGN---QNSRIERSNNGQPRRPNRSNRNRNNAVLSAERRNESLQPMDLPRGYEMRKTQQGQVYFYHVPTGTSTWHDPRIPRDLPANELTTEL-----------GPLPSGWEMRQTQSG---------------RVYFVDHNNRTTQFTDPRLSSQII------------SNLLNRRQN--NSTNNQVSGNPNNSPAATNEPVTVDTPVSSIPPQISTNQQRPRIENGSNNNSPTLETST---PPTQTVSDLPKELMES---------------------------ELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLSDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGVLKNHELKPGGKDIQVTEENKKEYVRLYVNYRFMRGIEQQFLALQKGFHELIPPSLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGASGPRLFTIHAVDAPSENLPKAHTCFNRIDIPQSYPCYQKMLDKLTQAVEETCGFAVE 908          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. nr
Match: gi|954547610|ref|XP_014616351.1| (PREDICTED: E3 ubiquitin-protein ligase SMURF2 isoform X2 [Polistes canadensis])

HSP 1 Score: 884.404 bits (2284), Expect = 0.000e+0
Identity = 504/996 (50.60%), Postives = 608/996 (61.04%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLV-TDPHHAWPVKGQIVISLLSRDGQG-----TGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERPS-RPALSDLSLNSIPGLQHQISSTSTASGGSASGALAPPTQRNGHAGEGRS-----GRGGSRRTNGTSAAAAGPVANPSTVISPIGLPDGFEIRHT-------EQGQIYFINVQTGVSTWHDPRIPKDYN----------LSEVFGGSSNMSNDEVLGPM--PTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHV-LQKIQTHFQQRSGPPPQTEGPPPSAPVMESNPPGASPERPAPKTSEGAPRTAEAGANVDQTEPRSPTEESETTRPPLVPKTNGETSNNNNRSHSTSSSSSNQASSQPATSLANGHTPAV--TPTSAAAEAAAPGASQPSTPSATSASQSTVLSNTVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNPSHHDNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLP-AYESQTKMHDKLTQAVEETCGFAVE 980
            G  KIR+TILCA NLA++DLFRLPDPF K++VD +G          Q H TDT + TL PKWN HYDL +   D ITISVWN +K    +G          GFLGCVR+LSN ++RLKDTGYQRLDL          VKGQIV+SLLSRDG       T   NAVVD LG+L C  +LP+GWEERR  SGR+YY+NH TR TQW RP+ RP+      N+I     +      +   S SG+ +PP  R       +S     G G S RT    ++A     N  T            +R+T       ++  +   + Q G     + RI +  N           +      S    +E L PM  P G+E R+T  G+ YF    + T+ + DPR+   +   ++ T             GP PS   M     G               R      N   T+   P   S+     L  + N               S++NQ S  P  S A  + P    TP S+     +    +P   + ++ +  T+ ++T   PPT +V+ +      S                           E+LPKYKRDLVAK+K LR EL + QPQSGHCRLEVSR E+FEESYR I+KMRPKD++KRLM+KFRGE+GLDYGG+AREWL+LLSHEMLNP YGLFQYSRDD YTLQINPDS INP+HLSYFHF GRIIG+AVFHGHHIDGGFTTPFYKMLLNK I L DIE VDP LH SL WMLEN I G+LD+ FSVEH +FG+L++ ELK GGKD++VTE+NKKEYV+LYVNYR+ RGIEQQFLALQKGF+ELIP  LL+PFDERELELVIGG+G IDI DWK HTRLKHC  DTPVVKWFW+IV+ Y EEMRARLLQFVTGSSRVPLQGFKALQGSTGA+GPRLFT+H ++   ENLPKAHTCFNR+D+P +Y    KM DKLTQAVEETCGFAVE
Sbjct:   11 GAMKIRLTILCARNLARKDLFRLPDPFAKITVDGSG----------QCHSTDTCKATLAPKWNQHYDLYIGKNDGITISVWNHRKIHKKQG---------GGFLGCVRILSNTIQRLKDTGYQRLDLCKAHSEDTDVVKGQIVVSLLSRDGHNGAVSNTVGHNAVVDVLGDLSCPTDLPDGWEERRTQSGRLYYINHYTRTTQWIRPNNRPS------NAIIPTTPEPPPLPNSEPTSPSGSGSPPNVRTDSPTVLQSTPEWNGEGTSSRTPSRDSSAQNTPRNTPTQQQNRSQQSQQHMRNTPPSSAIRDRRPVRSSDEQNGN---QNSRIERSNNGQPRRPNRSNRNRNNAVLSAERRNESLQPMDLPRGYEMRKTQQGQVYFYHVPTGTSTWHDPRIPRDLPANELTTEL-----------GPLPSGWEMRQTQSG---------------RVYFVDHNNRTTQFTDPRLSSQIISNLLKRQNN---------------STNNQVSGNPNNSPAATNEPVTVDTPVSSIPPQISTNQQRPRIENGSNNNSPTLETST---PPTQTVSDLPKELMES---------------------------ELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLSDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGVLKNHELKPGGKDIQVTEENKKEYVRLYVNYRFMRGIEQQFLALQKGFHELIPPSLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGASGPRLFTIHAVDAPSENLPKAHTCFNRIDIPQSYPCYQKMLDKLTQAVEETCGFAVE 907          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. nr
Match: gi|972174080|ref|XP_015179970.1| (PREDICTED: E3 ubiquitin-protein ligase SMURF1 [Polistes dominula] >gi|972174082|ref|XP_015179977.1| PREDICTED: E3 ubiquitin-protein ligase SMURF1 [Polistes dominula])

HSP 1 Score: 881.708 bits (2277), Expect = 0.000e+0
Identity = 509/995 (51.16%), Postives = 612/995 (61.51%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLV-TDPHHAWPVKGQIVISLLSRDGQG-----TGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERPS-RPALSDLSLNSIPGLQHQISSTSTASGGSASGALAPPTQRNGHAGEGRS-----GRGGSRRTNGTSAAAAGPVANPSTVISPIGLPDGFEIRHT-------EQGQIYFINVQTGVSTWHDPRIPKDYN----------LSEVFGGSSNMSNDEVLGP--MPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHV-LQKIQTHFQQRSGPPPQTEGPPPSAPVMESNPPGASPERPAPKTSEGAPRTAEAGANVDQTEPRSPTEESETTRPPLVPKTNGETSNNNNRSHSTSSSSSNQASSQPATSLANGHTPAVTPTSAAAEAAAPGASQPSTPSATSASQSTVLSN-TVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNPSHHDNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLP-AYESQTKMHDKLTQAVEETCGFAVE 980
            G  KIR+TILCA NLA++DLFRLPDPF K++VD +G          Q H TDT + TL PKWN HYDL +   D ITISVWN +K    +G          GFLGCVR+LSN ++RLKDTGYQRLDL          VKGQIV+SLLSRDG       T   NAVVD LG+L C  +LP+GWEERR   GR+YY+NH TR TQW RP+ RP+      N+I     +      +   S SG+ +PP  R       +S     G G S RT    ++A     N  T            IR+T       ++  +   + Q G     + RI +  N           +      S    +E L P  +P G+E R+T  G+ YF    + T+ + DPR+   +   ++ T             GP PS   M     G               R      N   T+   P   S+              SN  NR  +  +S++NQ S  P  S      PA T  S A E   P +S P  P  ++  Q   + N + N  PT   ++  T T S   + +                    ++E+LPKYKRDLVAK+K LR EL + QPQSGHCRLEVSR E+FEESYR I+KMRPKD++KRLM+KFRGE+GLDYGG+AREWL+LLSHEMLNP YGLFQYSRDD YTLQINPDS INP+HLSYFHF GRIIG+AVFHGHHIDGGFTTPFYKMLLNK I L DIE VDP LH SL WMLEN I G+LD+ FSVEH +FG+L++ ELK GGKD++VTE+NK+EYV+LYVNYR+ RGIEQQFLALQKGF+ELIP  LL+PFDERELELVIGG+G IDI DWK HTRLKHC  DTPVVKWFW+IV+ Y EEMRARLLQFVTGSSRVPLQGFKALQGSTGA+GPRLFT+H ++   ENLPKAHTCFNR+D+P +Y    KM DKLTQAVEETCGFAVE
Sbjct:   11 GAMKIRLTILCARNLARKDLFRLPDPFAKITVDGSG----------QCHSTDTCKATLAPKWNQHYDLYIGKNDGITISVWNHRKIHKKQG---------GGFLGCVRILSNTIQRLKDTGYQRLDLCKAHSEDTDVVKGQIVVSLLSRDGHNGAVSNTVVHNAVVDVLGDLSCPTDLPDGWEERRTQRGRLYYINHYTRTTQWIRPNNRPS------NAIIPTTPEPPPLPNSEPTSPSGSGSPPNVRTDSPTVLQSTPEWNGEGTSSRTPSRDSSAQNTPRNTPTQQQNRSQQSQQHIRNTPPSSAIRDRRPVRSNDEQDGN---QNSRIERSNNGQPRRPNRSNRNRNNAVLSAERRNESLQPTDLPRGYEMRKTQQGQVYFYHVPTGTSTWHDPRIPRDLPANELTTEL-----------GPLPSGWEMRQTQSG---------------RVYFVDHNNRTTQFTDPRLSSQII------------SNLLNRRQN--NSTNNQVSGNPNNS------PAATNESVAVET--PVSSIP--PQISTNQQRPRIENGSNNNSPTLETSTPPTQTVSDLPKEL-------------------MESELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLSDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGVLKNHELKPGGKDIQVTEENKREYVRLYVNYRFMRGIEQQFLALQKGFHELIPPSLLRPFDERELELVIGGLGTIDINDWKLHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGASGPRLFTIHAVDAPSENLPKAHTCFNRIDIPQSYPCYQKMLDKLTQAVEETCGFAVE 908          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. nr
Match: gi|817086932|ref|XP_012266218.1| (E3 ubiquitin-protein ligase SMURF2 [Athalia rosae] >gi|1183244073|ref|XP_020711414.1| E3 ubiquitin-protein ligase SMURF2 [Athalia rosae])

HSP 1 Score: 879.782 bits (2272), Expect = 0.000e+0
Identity = 511/997 (51.25%), Postives = 603/997 (60.48%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLV-TDPHHAWPVKGQIVISLLSRDGQG-----TGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERP-SRPA--LSDLSLNSIPGLQHQISSTSTASGGSASGALAPPTQRNG----HAGEGRSGRGGSR-RTNGTSAAAAGPVANPSTVISPIGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSS------------------NMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKIQTHFQQRSGPPPQTEGPPPSAPVME-SNPPGASPERPAPKTSEGAPRTAEAGANVDQTEPRSPTEESETTRPPLVPKTNGETSNNNNR-SHSTSSSSSNQASSQPATSLANGHTPAVTPTSAAAEAAAPGASQPSTPSATSASQSTVLSNTVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNPSH-HDNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPA-YESQTKMHDKLTQAVEETCGFAVE 980
            G  KIR+TILCA NLA++DLFRLPDPF K++VD +G          Q H T T   TLDPKWN HYDL +   D I IS+WN +K      +PG       GFLGCVR+LSN ++RLKDTGYQRLDL       A  VKGQIV+SLLSRDG       T   NAVVD LG+L C   LP+GWEERR  +GR+YY++H+ R TQW RP SRP   +   +L S+P           +   S SG+ +P   RN     H  E   G  G + RT     +A         VI          +R+T                   PR   + N + +  GS                      ND     +P G+E R+T  G+ YF    S  + + DPR+                         P   P  E +N  G  P                +G  + QT+         T R   V   N  T   + R S    S+   + S Q  TS     TP   P +   E   PG   P TP+ +S SQ+     T  A    S TS +TPT                N    ++ P    ++E+LPKYKRDLVAK+K LR EL + QPQSGHCRLEVSR E+FEESYR I+KMRPKD++KRLM+KFRGE+GLDYGG+AREWL+LLSHEMLNP YGLFQYSRDD YTLQINPDS INP+HLSYFHF GRIIG+AVFHGHH+DGGFTTPFYKMLLNK I L DIE VDP LH SL WMLEN I G+LD+ FSVEH +FG L++ ELK GGKD+ VTE+NK+EYV+LYVNYR+ RGIEQQFLALQKGF+ELIP QLL+PFDERELELVIGG+G IDI DWK HTRLKHC  DTP+VKWFW+IV+ Y EEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFT+H I+   ENLPKAHTCFNR+D+P  Y+S  KM DKLTQAVEETCGFAVE
Sbjct:   11 GAVKIRLTILCARNLARKDLFRLPDPFAKITVDGSG----------QCHSTATCTATLDPKWNQHYDLFIGKNDGIAISIWNRRKI---HKKPG------SGFLGCVRILSNTIQRLKDTGYQRLDLCKAHSDDADVVKGQIVVSLLSRDGHNGAVSNTVGHNAVVDVLGDLSCPTVLPDGWEERRTGNGRLYYIDHLNRSTQWVRPNSRPGNPIIPTTLESLPA--------PGSEPTSPSGSGSPQNTRNQSPTPHTPERNGGEVGQQNRTPVRENSACNTSTQSQNVIQ--------NLRNT--------TTNNSTRERRTPRSNDEQNGNRLPNGSQARRTNRTNRNRNSIMASGERRNDNQPSDLPRGYELRKTQQGQVYFYHVPSGASTWHDPRI-------------------------PRDLPANELANELGPLP----------------SGWEMRQTQ---------TGRVYFVDHNNRTTQFTDPRLSGQIISNLLKRQSPQNGTSSQVQSTPVRNPATPRLEL--PGNETPLTPAVSSPSQNLSRPRTEGASNNNSPTSESTPTQ---------------NGQTVSDLPKELMESELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHVDGGFTTPFYKMLLNKAITLCDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGALKNHELKPGGKDIAVTEENKREYVRLYVNYRFMRGIEQQFLALQKGFHELIPPQLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPIVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTIHAIDAPCENLPKAHTCFNRIDIPQNYQSYQKMLDKLTQAVEETCGFAVE 897          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. nr
Match: gi|571558557|ref|XP_006564410.1| (PREDICTED: E3 ubiquitin-protein ligase SMURF2 isoform X2 [Apis mellifera])

HSP 1 Score: 689.878 bits (1779), Expect = 0.000e+0
Identity = 334/459 (72.77%), Postives = 376/459 (81.92%), Query Frame = 0

Query:  535 SASQSTVLSNTVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNPSHH------------DNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLP-AYESQTKMHDKLTQAVEETCGFAVE 980
            S +Q+T  SN+ N+      T + TP S S   NI      RS + NG  N + +            DNE+LPKYKRDLVAK+K LR EL + QPQSGHCRLEVSR E+FEESYR I+KMRPKD++KRLM+KFRGE+GLDYGG+AREWL+LLSHEMLNP YGLFQYSRDD YTLQINPDS INP+HLSYFHF GRIIG+AVFHGHHIDGGFTTPFYKMLLNK I L DIE VDP LH SL WMLEN I G+LD+ FSVEH +FG+L++ ELK GGKD+ VTE+NKKEYV+LYVNYR+ RGIEQQFLALQKGF+ELIP QLL+PFDERELELVIGG+G IDI DWK HTRLKHC  DTPVVKWFW+IV+ Y EEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFT+H ++   ENLPKAHTCFNR+D+P +Y S  KM DKLTQAVEETCGFAVE
Sbjct:  445 STNQTT--SNSNNSATINESTEIETPASPSIQCNI---VQQRSRTENGMENTAQNAQTVSELPKELMDNELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLTDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGVLKNHELKPGGKDIPVTEENKKEYVRLYVNYRFMRGIEQQFLALQKGFHELIPPQLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTIHAVDAPSENLPKAHTCFNRIDIPESYPSYQKMLDKLTQAVEETCGFAVE 898          

HSP 2 Score: 211.075 bits (536), Expect = 6.655e-53
Identity = 117/211 (55.45%), Postives = 136/211 (64.45%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLV-TDPHHAWPVKGQIVISLLSRDGQG-----TGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERP-SRP 223
            G  KIR+TILCA NLA++DLFRLPDPF K++VD +G          Q H T T + TLDP WN HYDL +   D ITISVWN KK                GFLGCVR+LSN ++RLKDTGYQRLDL          VKG++V+SLLSRDG       T   NAVVD LG+L C  +LP+GWEERR  SGR+YYVNH TR TQW RP +RP
Sbjct:   11 GAMKIRLTILCARNLARKDLFRLPDPFAKITVDGSG----------QCHSTHTCKATLDPMWNQHYDLYIGKDDGITISVWNHKKI---------HKKKGGGFLGCVRILSNTIQRLKDTGYQRLDLCKAHSDDTDVVKGEVVVSLLSRDGHNGAVSNTVGHNAVVDVLGDLSCPTDLPDGWEERRTRSGRLYYVNHYTRTTQWIRPNARP 202          

HSP 3 Score: 115.546 bits (288), Expect = 1.879e-22
Identity = 54/99 (54.55%), Postives = 72/99 (72.73%), Query Frame = 0

Query:  298 PIGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSNMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKI 396
            P  LP G+E+R T+QGQ+YF +V TG STWHDPRIP+D   +E       ++N+  LGP+P+GWE R+T +GR YFVDH +RTTQFTDPRLS  ++  +
Sbjct:  349 PPDLPRGYEMRKTQQGQVYFYHVPTGSSTWHDPRIPRDLPANE-------LANE--LGPLPSGWEMRQTQSGRVYFVDHNNRTTQFTDPRLSSQIISNL 438          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. nr
Match: gi|1035609007|ref|XP_016912090.1| (PREDICTED: E3 ubiquitin-protein ligase SMURF1 isoform X2 [Apis cerana])

HSP 1 Score: 689.108 bits (1777), Expect = 0.000e+0
Identity = 334/465 (71.83%), Postives = 378/465 (81.29%), Query Frame = 0

Query:  535 SASQSTVLSNTVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNPSHH------------------DNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLP-AYESQTKMHDKLTQAVEETCGFAVE 980
            S +Q+T  SN+ N+      T + TP S S   NI     SR+ + + NN+P+                    DNE+LPKYKRDLVAK+K LR EL + QPQSGHCRLEVSR E+FEESYR I+KMRPKD++KRLM+KFRGE+GLDYGG+AREWL+LLSHEMLNP YGLFQYSRDD YTLQINPDS INP+HLSYFHF GRIIG+AVFHGHHIDGGFTTPFYKMLLNK I L DIE VDP LH SL WMLEN I G+LD+ FSVEH +FG+L++ ELK GGKD+ VTE+NKKEYV+LYVNYR+ RGIEQQFLALQKGF+ELIP QLL+PFDERELELVIGG+G IDI DWK HTRLKHC  DTPVVKWFW+IV+ Y EEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFT+H ++   ENLPKAHTCFNR+D+P +Y S  KM DKLTQAVEETCGFAVE
Sbjct:  444 STNQTT--SNSNNSATINESTEIETPASPSIQCNI-VQQRSRTENGSNNNSPTLENTSAQNAQTVSELPKELMDNELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLTDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGVLKNHELKPGGKDIPVTEENKKEYVRLYVNYRFMRGIEQQFLALQKGFHELIPPQLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTIHAVDAPSENLPKAHTCFNRIDIPESYPSYQKMLDKLTQAVEETCGFAVE 905          

HSP 2 Score: 211.075 bits (536), Expect = 6.601e-53
Identity = 117/211 (55.45%), Postives = 136/211 (64.45%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLV-TDPHHAWPVKGQIVISLLSRDGQG-----TGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERP-SRP 223
            G  KIR+TILCA NLA++DLFRLPDPF K++VD +G          Q H T T + TLDP WN HYDL +   D ITISVWN KK                GFLGCVR+LSN ++RLKDTGYQRLDL          VKG++V+SLLSRDG       T   NAVVD LG+L C  +LP+GWEERR  SGR+YYVNH TR TQW RP +RP
Sbjct:   11 GAMKIRLTILCARNLARKDLFRLPDPFAKITVDGSG----------QCHSTHTCKATLDPMWNQHYDLYIGKDDGITISVWNHKKI---------HKKKGGGFLGCVRILSNTIQRLKDTGYQRLDLCKAHSDDTDVVKGEVVVSLLSRDGHNGAVSNTVGHNAVVDVLGDLSCPTDLPDGWEERRTRSGRLYYVNHYTRTTQWIRPNARP 202          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. nr
Match: gi|1035609005|ref|XP_016912089.1| (PREDICTED: E3 ubiquitin-protein ligase SMURF1 isoform X1 [Apis cerana])

HSP 1 Score: 689.108 bits (1777), Expect = 0.000e+0
Identity = 334/465 (71.83%), Postives = 378/465 (81.29%), Query Frame = 0

Query:  535 SASQSTVLSNTVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNPSHH------------------DNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLP-AYESQTKMHDKLTQAVEETCGFAVE 980
            S +Q+T  SN+ N+      T + TP S S   NI     SR+ + + NN+P+                    DNE+LPKYKRDLVAK+K LR EL + QPQSGHCRLEVSR E+FEESYR I+KMRPKD++KRLM+KFRGE+GLDYGG+AREWL+LLSHEMLNP YGLFQYSRDD YTLQINPDS INP+HLSYFHF GRIIG+AVFHGHHIDGGFTTPFYKMLLNK I L DIE VDP LH SL WMLEN I G+LD+ FSVEH +FG+L++ ELK GGKD+ VTE+NKKEYV+LYVNYR+ RGIEQQFLALQKGF+ELIP QLL+PFDERELELVIGG+G IDI DWK HTRLKHC  DTPVVKWFW+IV+ Y EEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFT+H ++   ENLPKAHTCFNR+D+P +Y S  KM DKLTQAVEETCGFAVE
Sbjct:  445 STNQTT--SNSNNSATINESTEIETPASPSIQCNI-VQQRSRTENGSNNNSPTLENTSAQNAQTVSELPKELMDNELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLTDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGVLKNHELKPGGKDIPVTEENKKEYVRLYVNYRFMRGIEQQFLALQKGFHELIPPQLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTIHAVDAPSENLPKAHTCFNRIDIPESYPSYQKMLDKLTQAVEETCGFAVE 906          

HSP 2 Score: 211.46 bits (537), Expect = 6.355e-53
Identity = 117/211 (55.45%), Postives = 136/211 (64.45%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLV-TDPHHAWPVKGQIVISLLSRDGQG-----TGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERP-SRP 223
            G  KIR+TILCA NLA++DLFRLPDPF K++VD +G          Q H T T + TLDP WN HYDL +   D ITISVWN KK                GFLGCVR+LSN ++RLKDTGYQRLDL          VKG++V+SLLSRDG       T   NAVVD LG+L C  +LP+GWEERR  SGR+YYVNH TR TQW RP +RP
Sbjct:   11 GAMKIRLTILCARNLARKDLFRLPDPFAKITVDGSG----------QCHSTHTCKATLDPMWNQHYDLYIGKDDGITISVWNHKKI---------HKKKGGGFLGCVRILSNTIQRLKDTGYQRLDLCKAHSDDTDVVKGEVVVSLLSRDGHNGAVSNTVGHNAVVDVLGDLSCPTDLPDGWEERRTRSGRLYYVNHYTRTTQWIRPNARP 202          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. nr
Match: gi|328784531|ref|XP_396318.4| (PREDICTED: E3 ubiquitin-protein ligase SMURF2 isoform X1 [Apis mellifera])

HSP 1 Score: 689.108 bits (1777), Expect = 0.000e+0
Identity = 334/464 (71.98%), Postives = 378/464 (81.47%), Query Frame = 0

Query:  535 SASQSTVLSNTVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNPSHH-----------------DNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLP-AYESQTKMHDKLTQAVEETCGFAVE 980
            S +Q+T  SN+ N+      T + TP S S   NI     SR+ + + NN+P+                   DNE+LPKYKRDLVAK+K LR EL + QPQSGHCRLEVSR E+FEESYR I+KMRPKD++KRLM+KFRGE+GLDYGG+AREWL+LLSHEMLNP YGLFQYSRDD YTLQINPDS INP+HLSYFHF GRIIG+AVFHGHHIDGGFTTPFYKMLLNK I L DIE VDP LH SL WMLEN I G+LD+ FSVEH +FG+L++ ELK GGKD+ VTE+NKKEYV+LYVNYR+ RGIEQQFLALQKGF+ELIP QLL+PFDERELELVIGG+G IDI DWK HTRLKHC  DTPVVKWFW+IV+ Y EEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFT+H ++   ENLPKAHTCFNR+D+P +Y S  KM DKLTQAVEETCGFAVE
Sbjct:  445 STNQTT--SNSNNSATINESTEIETPASPSIQCNI-VQQRSRTENGSNNNSPTLENTAQNAQTVSELPKELMDNELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLTDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGVLKNHELKPGGKDIPVTEENKKEYVRLYVNYRFMRGIEQQFLALQKGFHELIPPQLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTIHAVDAPSENLPKAHTCFNRIDIPESYPSYQKMLDKLTQAVEETCGFAVE 905          

HSP 2 Score: 211.075 bits (536), Expect = 6.786e-53
Identity = 117/212 (55.19%), Postives = 136/212 (64.15%), Query Frame = 0

Query:   20 GVPKIRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLV-TDPHHAWPVKGQIVISLLSRDGQG-----TGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERP-SRPA 224
            G  KIR+TILCA NLA++DLFRLPDPF K++VD +G          Q H T T + TLDP WN HYDL +   D ITISVWN KK                GFLGCVR+LSN ++RLKDTGYQRLDL          VKG++V+SLLSRDG       T   NAVVD LG+L C  +LP+GWEERR  SGR+YYVNH TR TQW RP +RP 
Sbjct:   11 GAMKIRLTILCARNLARKDLFRLPDPFAKITVDGSG----------QCHSTHTCKATLDPMWNQHYDLYIGKDDGITISVWNHKKI---------HKKKGGGFLGCVRILSNTIQRLKDTGYQRLDLCKAHSDDTDVVKGEVVVSLLSRDGHNGAVSNTVGHNAVVDVLGDLSCPTDLPDGWEERRTRSGRLYYVNHYTRTTQWIRPNARPT 203          

HSP 3 Score: 115.546 bits (288), Expect = 1.930e-22
Identity = 54/99 (54.55%), Postives = 72/99 (72.73%), Query Frame = 0

Query:  298 PIGLPDGFEIRHTEQGQIYFINVQTGVSTWHDPRIPKDYNLSEVFGGSSNMSNDEVLGPMPTGWERRETSTGRPYFVDHLSRTTQFTDPRLSGHVLQKI 396
            P  LP G+E+R T+QGQ+YF +V TG STWHDPRIP+D   +E       ++N+  LGP+P+GWE R+T +GR YFVDH +RTTQFTDPRLS  ++  +
Sbjct:  349 PPDLPRGYEMRKTQQGQVYFYHVPTGSSTWHDPRIPRDLPANE-------LANE--LGPLPSGWEMRQTQSGRVYFVDHNNRTTQFTDPRLSSQIISNL 438          

BLAST of e3 ubiquitin-protein ligase smurf1 isoform x1 vs. nr
Match: gi|925676035|gb|KOX73303.1| (E3 ubiquitin-protein ligase SMURF2, partial [Melipona quadrifasciata])

HSP 1 Score: 688.723 bits (1776), Expect = 0.000e+0
Identity = 334/466 (71.67%), Postives = 376/466 (80.69%), Query Frame = 0

Query:  534 TSASQSTVLSNTVNAPPTTSVTSVTTPTSSSSIRNIRATSYSRSNSTNGNNNPSHH------------------DNEVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLP-AYESQTKMHDKLTQAVEETCGFAVE 980
            ++ +Q+T  SN   A    S   + TP SSS   NI        N +N NN+P+                    DNE+LPKYKRDLVAK+K LR EL + QPQSGHCRLEVSR E+FEESYR I+KMRPKD++KRLM+KFRGE+GLDYGG+AREWL+LLSHEMLNP YGLFQYSRDD YTLQINPDS INP+HLSYFHF GRIIG+AVFHGHHIDGGFTTPFYKMLLNK I L DIE VDP LH SL WMLEN I G+LD+ FSVEH +FG+L++ ELK GGKD+ VTE+NKKEYV+LYVNYR+ RGIEQQFLALQKGF+ELIP QLL+PFDERELELVIGG+G IDI DWK HTRLKHC  DTPVVKWFW+IV+ Y EEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFT+H ++   ENLPKAHTCFNR+D+P +Y S  KM DKLTQAVEETCGFAVE
Sbjct:  430 STINQTTSNSNNSTAAANESA-EIETPVSSSIQSNIVQQRQRTENGSN-NNSPTLENTSTQNAQTVSELPKELMDNELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLTDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGVLKNHELKPGGKDIPVTEENKKEYVRLYVNYRFMRGIEQQFLALQKGFHELIPPQLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTIHAVDAPSENLPKAHTCFNRIDIPESYPSYQKMLDKLTQAVEETCGFAVE 893          

HSP 2 Score: 204.912 bits (520), Expect = 8.331e-51
Identity = 112/207 (54.11%), Postives = 132/207 (63.77%), Query Frame = 0

Query:   24 IRVTILCACNLAKRDLFRLPDPFVKLSVDPAGGLPPGRPLWGQMHCTDTDRGTLDPKWNAHYDLMLRPTDVITISVWNEKKAKGGEGRPGHSLTLTDGFLGCVRLLSNAVERLKDTGYQRLDLV-TDPHHAWPVKGQIVISLLSRDGQG-----TGSLNAVVDSLGNLRCANELPEGWEERRATSGRIYYVNHVTRCTQWERP-SRP 223
            +  T+LCA NLA++DLFRLPDPF K++VD +G          Q H T T + TLDP WN HYDL +   D ITISVWN KK                GFLGCVR+LSN ++RLKDTGYQRLDL          +KG++VISLLSRDG       T   NAVVD LG+L C  +LP+GWEERR  SGR+YYVNH TR TQW RP +RP
Sbjct:    1 VNFTVLCARNLARKDLFRLPDPFAKITVDGSG----------QCHSTHTCKATLDPMWNQHYDLYIGKDDGITISVWNHKKI---------HKKKGGGFLGCVRILSNTIQRLKDTGYQRLDLCKAHSDDTDVIKGEVVISLLSRDGHNGAVSNTVGHNAVVDVLGDLSCPTDLPDGWEERRTRSGRLYYVNHYTRTTQWIRPNARP 188