EMLSAG00000000708, EMLSAG00000000708-683474 (gene) Lepeophtheirus salmonis

Overview
NameEMLSAG00000000708
Unique NameEMLSAG00000000708-683474
Typegene
OrganismLepeophtheirus salmonis (salmon louse)
Associated RNAi Experiments

Nothing found

Homology
[Close]
BLAST of EMLSAG00000000708 vs. C. finmarchicus
Match: gi|592830705|gb|GAXK01126839.1| (TSA: Calanus finmarchicus comp47903_c0_seq5 transcribed RNA sequence)

HSP 1 Score: 47.7506 bits (112), Expect = 9.708e-5
Identity = 23/46 (50.00%), Postives = 32/46 (69.57%), Query Frame = 0 
Query:  174 SCPTITNRPPVSACQGAKSNCWSVGEAXWDCPNNEFCXFDGCANTC 219
            SCP+++  P  S C+G+ S+CWS G+   DCP+   C FDGC+NTC
Sbjct:  489 SCPSLSPLP-SSDCEGSVSSCWSPGQPDGDCPDFGLCCFDGCSNTC 623

HSP 2 Score: 35.039 bits (79), Expect = 1.023e+0
Identity = 17/33 (51.52%), Postives = 21/33 (63.64%), Query Frame = 0 
Query:  532 CDGEVDTCWNTGLRD-DCSLGFICCFDGCVKTC 563
            C+G V +CW+ G  D DC    +CCFDGC  TC
Sbjct:  525 CEGSVSSCWSPGQPDGDCPDFGLCCFDGCSNTC 623
[Close]
BLAST of EMLSAG00000000708 vs. C. finmarchicus
Match: gi|592830706|gb|GAXK01126838.1| (TSA: Calanus finmarchicus comp47903_c0_seq4 transcribed RNA sequence)

HSP 1 Score: 47.7506 bits (112), Expect = 1.078e-4
Identity = 23/46 (50.00%), Postives = 32/46 (69.57%), Query Frame = 0 
Query:  174 SCPTITNRPPVSACQGAKSNCWSVGEAXWDCPNNEFCXFDGCANTC 219
            SCP+++  P  S C+G+ S+CWS G+   DCP+   C FDGC+NTC
Sbjct:  489 SCPSLSPLP-SSDCEGSVSSCWSPGQPDGDCPDFGLCCFDGCSNTC 623

HSP 2 Score: 35.039 bits (79), Expect = 1.081e+0
Identity = 17/33 (51.52%), Postives = 21/33 (63.64%), Query Frame = 0 
Query:  532 CDGEVDTCWNTGLRD-DCSLGFICCFDGCVKTC 563
            C+G V +CW+ G  D DC    +CCFDGC  TC
Sbjct:  525 CEGSVSSCWSPGQPDGDCPDFGLCCFDGCSNTC 623
[Close]
BLAST of EMLSAG00000000708 vs. C. finmarchicus
Match: gi|592902589|gb|GAXK01055786.1| (TSA: Calanus finmarchicus comp3324309_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 45.4394 bits (106), Expect = 1.105e-4
Identity = 23/60 (38.33%), Postives = 32/60 (53.33%), Query Frame = 0 
Query:  776 CPSAMKCVPKQNCDFNGVM------------------PCVNQNSGTIDVCCRDPNYKXPW 817
            CPSAMKCVP+ NCD  GV+                  P ++  +G + VCCRDP+++  W
Sbjct:  227 CPSAMKCVPRDNCDLQGVISQKKVILPTSMEILFLRAPLID-CAGDLHVCCRDPDFEYLW 403
[Close]
BLAST of EMLSAG00000000708 vs. C. finmarchicus
Match: gi|592830707|gb|GAXK01126837.1| (TSA: Calanus finmarchicus comp47903_c0_seq3 transcribed RNA sequence)

HSP 1 Score: 47.3654 bits (111), Expect = 1.265e-4
Identity = 23/46 (50.00%), Postives = 32/46 (69.57%), Query Frame = 0 
Query:  174 SCPTITNRPPVSACQGAKSNCWSVGEAXWDCPNNEFCXFDGCANTC 219
            SCP+++  P  S C+G+ S+CWS G+   DCP+   C FDGC+NTC
Sbjct:  489 SCPSLSPLP-SSDCEGSVSSCWSPGQPDGDCPDFGLCCFDGCSNTC 623

HSP 2 Score: 34.6538 bits (78), Expect = 1.207e+0
Identity = 17/33 (51.52%), Postives = 21/33 (63.64%), Query Frame = 0 
Query:  532 CDGEVDTCWNTGLRD-DCSLGFICCFDGCVKTC 563
            C+G V +CW+ G  D DC    +CCFDGC  TC
Sbjct:  525 CEGSVSSCWSPGQPDGDCPDFGLCCFDGCSNTC 623
[Close]
BLAST of EMLSAG00000000708 vs. C. finmarchicus
Match: gi|592830708|gb|GAXK01126836.1| (TSA: Calanus finmarchicus comp47903_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 47.3654 bits (111), Expect = 1.268e-4
Identity = 23/46 (50.00%), Postives = 32/46 (69.57%), Query Frame = 0 
Query:  174 SCPTITNRPPVSACQGAKSNCWSVGEAXWDCPNNEFCXFDGCANTC 219
            SCP+++  P  S C+G+ S+CWS G+   DCP+   C FDGC+NTC
Sbjct:  489 SCPSLSPLP-SSDCEGSVSSCWSPGQPDGDCPDFGLCCFDGCSNTC 623

HSP 2 Score: 34.6538 bits (78), Expect = 1.209e+0
Identity = 17/33 (51.52%), Postives = 21/33 (63.64%), Query Frame = 0 
Query:  532 CDGEVDTCWNTGLRD-DCSLGFICCFDGCVKTC 563
            C+G V +CW+ G  D DC    +CCFDGC  TC
Sbjct:  525 CEGSVSSCWSPGQPDGDCPDFGLCCFDGCSNTC 623
[Close]
BLAST of EMLSAG00000000708 vs. L. salmonis peptides
Match: EMLSAP00000000708 (pep:novel supercontig:LSalAtl2s:LSalAtl2s110:876596:884706:-1 gene:EMLSAG00000000708 transcript:EMLSAT00000000708 description:"maker-LSalAtl2s110-augustus-gene-8.13")

HSP 1 Score: 1558.12 bits (4033), Expect = 0.000e+0
Identity = 821/821 (100.00%), Postives = 821/821 (100.00%), Query Frame = 0 
Query:    1 MKCLTFTSAICLLIAPALANEQYWWQDNPNVFSQGGGGSPSNGGNSFGGSRPQSSSFNNNNNNNGPSSDNSLSHELLTECPAGTRCTVDYYCDINAVISSVPVKLTKEDLRKRGHMIPCWHEESDKFEICCSXATGFASSSSPSFSSSPSFSSSNPSIVSAKLTLPNRTLAPGSCPTITNRPPVSACQGAKSNCWSVGEAXWDCPNNEFCXFDGCANTCYFGPASGGESSPSGSNQNSNQQGGRIPRKPNGNNNRGSSSQSSNSFSGPSGQPGSNNEGFPSNSGFLTGNGGHSGKPGSSNNNGSPSNSGFQPGSGGHSGTPGSNHNNRGSPSSSGFQPGNGGHSGKNNRGSSSQNSNSSPIPSGHPGNNNRGSSSSSGFQPGSGGHSSKPGFSSGSNNEGFPSNSGFQPGNGEHFNNNNRGSSNQPENNGNTGQRTDDSPVVFPGSSPNKNNGFPSSTNNQPSSGSYSPSTGSQNSPSVSVSCNDKRKCGNVQVVVSGSGNDPPPRPTPRPSGNVIRNSNCPNNPSPPTGICDGEVDTCWNTGLRDDCSLGFICCFDGCVKTCYNGTDFLRHLDSPNLIRGTGSNNFQSPSKQQQQSPSGFPAPTFAASQGNPGQPRPSSNNEFNSPNNNRQQPSGSFPSQNHNGQLQPPASGFSPPNQNRQPSQPNNGYLSPGSGNNNRQPSRPNNNGFPSSNNNNRQPSGTNNGVSSPSRGQPGNGFPNPNKFGQNPQSGSNNRFPSSGNNNNIGGQPGRPTGQRPSGSNNNFANXATKPYKSCPSAMKCVPKQNCDFNGVMPCVNQNSGTIDVCCRDPNYKXPWPQNN 821
            MKCLTFTSAICLLIAPALANEQYWWQDNPNVFSQGGGGSPSNGGNSFGGSRPQSSSFNNNNNNNGPSSDNSLSHELLTECPAGTRCTVDYYCDINAVISSVPVKLTKEDLRKRGHMIPCWHEESDKFEICCSXATGFASSSSPSFSSSPSFSSSNPSIVSAKLTLPNRTLAPGSCPTITNRPPVSACQGAKSNCWSVGEAXWDCPNNEFCXFDGCANTCYFGPASGGESSPSGSNQNSNQQGGRIPRKPNGNNNRGSSSQSSNSFSGPSGQPGSNNEGFPSNSGFLTGNGGHSGKPGSSNNNGSPSNSGFQPGSGGHSGTPGSNHNNRGSPSSSGFQPGNGGHSGKNNRGSSSQNSNSSPIPSGHPGNNNRGSSSSSGFQPGSGGHSSKPGFSSGSNNEGFPSNSGFQPGNGEHFNNNNRGSSNQPENNGNTGQRTDDSPVVFPGSSPNKNNGFPSSTNNQPSSGSYSPSTGSQNSPSVSVSCNDKRKCGNVQVVVSGSGNDPPPRPTPRPSGNVIRNSNCPNNPSPPTGICDGEVDTCWNTGLRDDCSLGFICCFDGCVKTCYNGTDFLRHLDSPNLIRGTGSNNFQSPSKQQQQSPSGFPAPTFAASQGNPGQPRPSSNNEFNSPNNNRQQPSGSFPSQNHNGQLQPPASGFSPPNQNRQPSQPNNGYLSPGSGNNNRQPSRPNNNGFPSSNNNNRQPSGTNNGVSSPSRGQPGNGFPNPNKFGQNPQSGSNNRFPSSGNNNNIGGQPGRPTGQRPSGSNNNFANXATKPYKSCPSAMKCVPKQNCDFNGVMPCVNQNSGTIDVCCRDPNYKXPWPQNN
Sbjct:    1 MKCLTFTSAICLLIAPALANEQYWWQDNPNVFSQGGGGSPSNGGNSFGGSRPQSSSFNNNNNNNGPSSDNSLSHELLTECPAGTRCTVDYYCDINAVISSVPVKLTKEDLRKRGHMIPCWHEESDKFEICCSXATGFASSSSPSFSSSPSFSSSNPSIVSAKLTLPNRTLAPGSCPTITNRPPVSACQGAKSNCWSVGEAXWDCPNNEFCXFDGCANTCYFGPASGGESSPSGSNQNSNQQGGRIPRKPNGNNNRGSSSQSSNSFSGPSGQPGSNNEGFPSNSGFLTGNGGHSGKPGSSNNNGSPSNSGFQPGSGGHSGTPGSNHNNRGSPSSSGFQPGNGGHSGKNNRGSSSQNSNSSPIPSGHPGNNNRGSSSSSGFQPGSGGHSSKPGFSSGSNNEGFPSNSGFQPGNGEHFNNNNRGSSNQPENNGNTGQRTDDSPVVFPGSSPNKNNGFPSSTNNQPSSGSYSPSTGSQNSPSVSVSCNDKRKCGNVQVVVSGSGNDPPPRPTPRPSGNVIRNSNCPNNPSPPTGICDGEVDTCWNTGLRDDCSLGFICCFDGCVKTCYNGTDFLRHLDSPNLIRGTGSNNFQSPSKQQQQSPSGFPAPTFAASQGNPGQPRPSSNNEFNSPNNNRQQPSGSFPSQNHNGQLQPPASGFSPPNQNRQPSQPNNGYLSPGSGNNNRQPSRPNNNGFPSSNNNNRQPSGTNNGVSSPSRGQPGNGFPNPNKFGQNPQSGSNNRFPSSGNNNNIGGQPGRPTGQRPSGSNNNFANXATKPYKSCPSAMKCVPKQNCDFNGVMPCVNQNSGTIDVCCRDPNYKXPWPQNN 821
[Close]
BLAST of EMLSAG00000000708 vs. L. salmonis peptides
Match: EMLSAP00000012204 (pep:novel supercontig:LSalAtl2s:LSalAtl2s884:108860:111527:1 gene:EMLSAG00000012204 transcript:EMLSAT00000012204 description:"maker-LSalAtl2s884-augustus-gene-1.10")

HSP 1 Score: 108.997 bits (271), Expect = 3.405e-25
Identity = 66/185 (35.68%), Postives = 91/185 (49.19%), Query Frame = 0 
Query:   56 SFNNNNNNNGPSSDNSLSHELLTECPAGTRCTVDYYCDINAVISSVPVKLTKEDLRKRGHMIPCWHEESDKFEICCSXATGFASSSSPSFSSSPSFSSSNPSIVSAKLTLPNRTLAP-GSCPTITNRPPVSACQGAKSNCWSVGEAXWDCPNNEFCXFDGCANTCY--FGPASGGESSPSGSNQN 237
            +F   N +NG +S +    E+ TEC AG+ CTVD +CD+N  ISSVP+ LT+E+   RG +IPCWH E+ + ++CC                       NP     K  + +  L    +CP +      S+C    S C SVG+    C NNE C +DGCAN C     P    E+SPS +  N
Sbjct:   47 AFQYTNFHNGSASYSP--PEIRTECQAGSYCTVDMFCDVNVTISSVPLNLTEEEKDDRGELIPCWHVETQEIQVCC-----------------------NPPSDEPKENVKSEDLKDFATCPRVPRAFSESSCSDVASTCASVGKPDQSCLNNELCCYDGCANRCISDLSPEPVEETSPSEAVSN 206

HSP 2 Score: 61.2326 bits (147), Expect = 5.644e-10
Identity = 28/63 (44.44%), Postives = 37/63 (58.73%), Query Frame = 0 
Query:  776 CPSAMKCVPKQNCDFNGV-------------------MPCVNQNSGTIDVCCRDPNYKXPWPQ 819
            CPSA+ CVPK+NCDFNGV                   +PC++  +G+ +VCCRDPNY  P+ Q
Sbjct:  424 CPSALSCVPKKNCDFNGVIVSHVVSTSPEFDTVRVPLLPCIHPQTGSSNVCCRDPNYVDPYSQ 486
[Close]
BLAST of EMLSAG00000000708 vs. Select Arthropod Genomes
Match: EEB19342.1 (trypsin, putative [Pediculus humanus corporis])

HSP 1 Score: 59.3066 bits (142), Expect = 5.375e-8
Identity = 26/70 (37.14%), Postives = 35/70 (50.00%), Query Frame = 0 
Query:  770 TKPYKSCPSAMKCVPKQNCDFNGVM-------------------PCVNQNSGTIDVCCRDPNYKXPWPQN 820
            T P+  CP+A+KCV +  C ++G+M                    C N  +G I  CCRDPNYK PWP +
Sbjct:  602 TTPHVGCPAALKCVEESYCTWDGIMSETPVTLTREEAENKVPLSDCQNIETGVIGKCCRDPNYKDPWPSD 671
[Close]
BLAST of EMLSAG00000000708 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold230_size244653-snap-gene-0.16 (protein:Tk08243 transcript:maker-scaffold230_size244653-snap-gene-0.16-mRNA-1 annotation:"serine proteinase stubble-like")

HSP 1 Score: 133.265 bits (334), Expect = 3.396e-32
Identity = 83/222 (37.39%), Postives = 117/222 (52.70%), Query Frame = 0 
Query:   76 LLTECPAGTRCTVDYYCDINAVISSVPVKLTKEDLRKRGHMIPCWHEESDKFEICCSXAT----------GFASSSSPSFS---SSPSFS----SSNPSIVSAKLTLPNRTLAPGSCPTITNRPPVSACQGAKSNCWSVGEAXWDCPNNEFCXFDGCANTCYFGPASGGESSPSGSNQNSN-----QQGGRIPRKPNGNNNRGSSSQSSNSFSGPSGQPGSN 275
            + TECPAGT+C  D++CD NA++ +  V L+    R RG+++PC ++++ +F++CCS A+               + PSF    + PSF     SS  +       + N ++  GSCP I   P +++C G KSNCWSVG+A  DCPNN  C FDGC N CYFG  +G    P  + Q SN     QQ    P     NN    + Q SN+   P+ QP  N
Sbjct:  108 VQTECPAGTKCVGDFFCDENAIMVNYRVDLSPAQKRNRGNLLPCMNQQTGQFDVCCSTASPGSQGNNRPSSQGQQNQPSFQGQQNQPSFQGQSGSSQNNNNPGNNQISN-SIPQGSCPNIGALPDIASCNGMKSNCWSVGQADVDCPNNALCCFDGCRNACYFGAGNGNAPPPRPAPQQSNNRPAPQQSNNRPAPQQSNNRPAPAPQQSNNRPAPAPQPSFN 328

HSP 2 Score: 78.5666 bits (192), Expect = 3.788e-15
Identity = 33/55 (60.00%), Postives = 38/55 (69.09%), Query Frame = 0 
Query:  520 NCPNNPSPPTGICDGEVDTCWNTGLRDDCSLGFICCFDGCVKTCYNGT--DFLRH 572
            +CPN    P+  C  E DTCWNTG RDDC+ GF+CC+DGC KTC N    DFLRH
Sbjct:  398 DCPNMSPLPSADCAAERDTCWNTGKRDDCARGFLCCYDGCSKTCLNTQTGDFLRH 452
[Close]
BLAST of EMLSAG00000000708 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold120_size336265-snap-gene-0.6 (protein:Tk08782 transcript:maker-scaffold120_size336265-snap-gene-0.6-mRNA-1 annotation:"hypothetical protein")

HSP 1 Score: 50.447 bits (119), Expect = 3.496e-7
Identity = 24/43 (55.81%), Postives = 27/43 (62.79%), Query Frame = 0 
Query:  187 CQGAKSNCWSVGEAXWDCPNNEFCXFDGCANTCYFGPASGGES 229
            C G  SNCWS G+   DCPN   C FDGCANTC  GP +  +S
Sbjct:   91 CAGTVSNCWSPGQGDTDCPNFGLCCFDGCANTCVDGPTAISQS 133
The following BLAST results are available for this feature:
BLAST of EMLSAG00000000708 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000000708 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA)
Total hits: 25
Match NameE-valueIdentityDescription
gi|592830705|gb|GAXK01126839.1|9.708e-550.00TSA: Calanus finmarchicus comp47903_c0_seq5 transc... [more]
gi|592830706|gb|GAXK01126838.1|1.078e-450.00TSA: Calanus finmarchicus comp47903_c0_seq4 transc... [more]
gi|592902589|gb|GAXK01055786.1|1.105e-438.33TSA: Calanus finmarchicus comp3324309_c0_seq1 tran... [more]
gi|592830707|gb|GAXK01126837.1|1.265e-450.00TSA: Calanus finmarchicus comp47903_c0_seq3 transc... [more]
gi|592830708|gb|GAXK01126836.1|1.268e-450.00TSA: Calanus finmarchicus comp47903_c0_seq2 transc... [more]

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BLAST of EMLSAG00000000708 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self)
Total hits: 2
Match NameE-valueIdentityDescription
EMLSAP000000007080.000e+0100.00pep:novel supercontig:LSalAtl2s:LSalAtl2s110:87659... [more]
EMLSAP000000122043.405e-2535.68pep:novel supercontig:LSalAtl2s:LSalAtl2s884:10886... [more]
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BLAST of EMLSAG00000000708 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000000708 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods)
Total hits: 1
Match NameE-valueIdentityDescription
EEB19342.15.375e-837.14trypsin, putative [Pediculus humanus corporis][more]
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BLAST of EMLSAG00000000708 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017))
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000000708 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins)
Total hits: 2
Match NameE-valueIdentityDescription
maker-scaffold230_size244653-snap-gene-0.163.396e-3237.39protein:Tk08243 transcript:maker-scaffold230_size2... [more]
maker-scaffold120_size336265-snap-gene-0.63.496e-755.81protein:Tk08782 transcript:maker-scaffold120_size3... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
LSalAtl2s110supercontigLSalAtl2s110:876596..884706 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
ensembl2013-09-26 .965016
Blast vs. GO2014-04-02
TblastN vs C. finmarchicus TSA2014-05-09
Blastp vs. self2014-05-10
Blastp vs. SwissProt2017-02-10
Blastp vs. Selected Arthropods2017-02-20
Blastp vs. NR (2/2017)2017-02-20
Blastp vs. Tigriopus kingsejongensis proteins2018-04-18
Properties
Property NameValue
Logic nameensemblgenomes
Descriptionmaker-LSalAtl2s110-augustus-gene-8.13
Biotypeprotein_coding
EvidenceIEA
NoteHypothetical protein
Cross References
External references for this gene
DatabaseAccession
Ensembl Metazoa (gene)EMLSAG00000000708 (primary cross-reference)
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
EMLSAT00000000708EMLSAT00000000708-696555Lepeophtheirus salmonismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at LSalAtl2s110:876596..884706-

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>EMLSAG00000000708-683474 ID=EMLSAG00000000708-683474|Name=EMLSAG00000000708|organism=Lepeophtheirus salmonis|type=gene|length=8111bp|location=Sequence derived from alignment at LSalAtl2s110:876596..884706- (Lepeophtheirus salmonis)
GAAATGAAGTGTCTAACATTTACATCGGCAATTTGCCTTCTCATTGCTCC
AGCCTTAGCTAATGAGCAATACTGGTGGCAGGACAACCCAAATGTATTTT
CTCAGGGTGGTGGAGGTGGCGGAGGATCTAATGGACAGGGGCCTCGAGGK
TTGTTGTCTAATTTTTATTTGGCTATTTTGTTTTATAAATAACTTTTTTA
TATTTTTAAAGGATCTCCTAGTAATGGAGGAAACTCCTTTGGTGGTTCCC
GTCCTCAATCGTCGTCCTTCAATAACAACAACAATAATAATGGGCCATCA
AGTGGTAAGTTTTTTTTGTTTTTTTTTCATTCACTTTATGATGTAATAAC
TTAATGCTAATTCACAAAAGTGTGCAATCCATCTACTTCCAAAAGTTTAT
ATACATAAAAGACTTAATTTATTTTGCAATTTTCAATAAAAAATCTACTA
ATCCAAAATTTTGTAGATCTTTAATTCCATACTTCAATTAAGATCTCTCT
ATTGTAATCCCTTATCATTTTTTAGACAACTCTCTTAGCCACGAACTATT
GACTGAGTGTCCAGCAGGTACTCGCTGCACTGTCGACTACTATTGTGATA
TCAATGCTGTAATTTCATCCGTACCAGTTAAACTAACAAAGGAGGACTTG
CGTAAACGTGGGCATATGATTGTGAGGAAACAAAAAAAGAACTTTTTACT
TTTTAAAAGACACTTTTAACCCATGACTTAAAAAATTTATAATCAGTAGA
TTCCACTTAAYGGGCATCGTTTGACACGTTTTAAAGACATTTTTAAAAGG
AATACTCGGGAAACTCCGTATATTTACCTCATAAGTATACATAGACTATT
AAATCCGAATAATAGTTTTGTATTAATTCATTATAAATGTTACTCTCATA
TCCGATCTGCATCTATAACTTGGTTTTATAGTCTGCATAGATCCTTATCA
ATTTTAAGTACTATTTAGGCCAATTTTTCTTAATTTTTACTTTTAAATTA
TTTTTCCATCTATCTCACCCAACAACAGTGAAAATATGGTTTCCATATTT
GTGAACATTCTGTGTCGTTATTATATGAGGCTCAGAAGTAAAATTTGTGA
AAGAAAAAGGTTCTCTAATTTTTTTATTATAGAATATATGTGTGAACTTA
ATGAAGTTGAGATATTGTAGAATTACACAAACATAGCTTTTATTTTCATT
ATTAATGATGAATAATACAAAAATAATCTTAATCAAAGATCAATAGTGAA
GAAATGAACATGTTTCCAATGCTGGAAGGTTTAAAAAAAAATGTAAACTA
TACTTTGGAACATTCAATTTAAGTGCAATGTGCAACGTCTGTAATGTCTT
CATCAAAATATCATAGAAATTGATGATAATTCGTCAAAGACTAAAAATAC
AACTTAGACTCAATTTCTGATCACAATTGTTCACACTTATTTGTGATTAA
TGGTTTACATTCATAGTAATGTTGCTGTTTATTTGAATTAATTTATTTAG
TTGTATCGGTCCATATTTAGGACTAAAAACTGCAGTCCTGTTCAGTTCTG
TCTTCGTTCGGTGGTCGAGTCCAGTCTTTAAATATATAAAGTTTGGTCCT
TGATTACGCCACTCAACATTATTTCTTCTTTTTTTAATAAGTCTTAGTAC
TGACAGTTTAAGGGACCAACATTCCTAAGGTAGAGTCCAAAGGACTGTTA
GAACTTAAGACTAGACTAGACTAATTGAATAAATAAGGACGGGCACGACA
GTCAAAACACTCAGAGTGATTTTCGCTCTAATCCGGATTTGACTGTATTT
TTAAAGATGTGAATCTGTTTTAGCTAAATAAAAGTAGAAGATTCAATTAA
TCCCATTAAGCAGAATTTACTGTGTCTCAAATTTTCTACAATACAAATAT
ATTATCGACTGTAATATTTTAGCCTTGCTGGCACGAAGAATCTGATAAAT
TTGAAATATGCTGTTCAMCAGCCACTGGTTTTGCTTCTTCTTCTTCTCCC
TCCTTCTCCTCTTCTCCTTCCTTTTCTTCTTCAAATCCCAGCATCGTATC
TGCTAAGCTTACTCTCCCAAATAGAACCCTTGGTAATTAATTCATAGCTT
ATAATTATTAATTTATATCTAACAAAACCTACTAACTTAATTGTACAATG
GAGTATGAGTGTGATTTACTTTATTATCCATTACTATAACTAAATACAGT
AGTGCCGCCTTCCGGAAAATCCATTAGCTTGCTCATACCCCATTTCCCAC
TAAAAATGAAGCTATGGAATATTTAATTTTTTTTAATAATAAACCCAATT
ATTGTCTTATTTCCAGCTCCAGGATCATGCCCCACCATAACGAACAGACC
CCCTGTCTCTGCTTGTCAAGGAGCCAAGTCCAACTGCTGGAGTGTTGGAG
AGGCTGRCTGGGACTGTCCCAACAATGAGTTCTGTYGTTTTGATGGATGT
GCCAATACATGTTACTTTGGACCAGGTGTGCATTTATTTTTAATCTGCTT
ATTTTTTCCTGCATTAATTTTTCAAAGAAATTTACTTTTAATAGCACTTT
GCATGATAATTTTTTGCACCCTAAATTGAATTAATTTCATTTTTTTTCCC
CTCTTGAATTGAATGAACTCCTAATTAAATGTCTTGTAACGGCCCTGAGT
AAAGAATTTGCCTTAAAAACTCCAATCACACTATTTCACTACCTATAAAA
TAATTTATTCCCAGCATCCGGAGGAGAATCATCTCCATCTGGATCAAATC
AAAACAGCAATCAACAAGGAGGAAGAATTCCCCGGAAGTTCATCAGGCTC
TACACCAGGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNA
GGCCCATCAACTAATAACAATAATAGACCCAATGGGAACAATAATCGTGG
ATCCTCTAGTCAATCTAGTAATAGTTTTTCCGGTCCCTCAGGACAACCTG
GGAGTAATAATGAAGGATTCCCTAGCAATTCTGGATTCCTAACTGGAAAT
GGAGGACACTCAGGCAAGCCTGGAAGTAGCAATAACAATGGATCTCCAAG
TAACTCTGGATTTCAGCCAGGAAGTGGAGGACATTCTGGTACACCTGGAA
GTAATCATAATAATAGAGGATCTCCAAGTAGTTCTGGATTCCAACCAGGA
AATGGAGGACACTCAGGCAAGAATAATAGAGGATCATCAAGTCAAAATAG
CAATAGTTCCCCCATTCCATCAGGACATCCAGGAAACAATAATAGGGGAT
CTTCAAGTAGTTCTGGTTTTCAACCAGGAAGTGGAGGCCATTCTAGCAAA
CCAGGATTTTCCTCTGGAAGCAACAACGAAGGGTTTCCAAGTAATTCAGG
ATTCCAACCAGGAAATGGAGAACATTTCAATAACAATAACAGAGGCTCAT
CCAATCAACCTGAAAACAATGGCAATACTGGACAAAGAACAGATGATTCT
CCAGTCGTATTTCCTGGATCTTCTCCAAACAAAAACAATGGATTTCCTTC
ATCAACTAACAACCAACCCTCATCTGGTTCATATTCTCCTTCTACTGGAT
CTCAAAATTCTCCCTCCGTAAGCGTTAGCTGTAATGATAAAAGAAAATGT
GGAAATGTCCAAGTCGTTGTCTCTGGCAGTGGTAATGATCCACCTCCAAG
ACCAACCCCAAGACCTTCGGGTAATGTAATACGAAACTCTAACTGTCCTA
ATAACCCATCTCCGCCTACCGGAATTTGTGATGGTGAAGTAGATACTTGT
TGGAATACGGGTCTTCGTGATGATTGCTCTCTAGGCTTTATTTGCTGCTT
TGATGGATGTGTTAAAACATGCTACAATGGAACAGATTTTCTAAGACATT
TAGATTCTCCTAACTTAATTAGAGGTGGTGCTTAAATATTAAAAACCTAA
ACATATAGGAACTGTACATATTTATGTAGTTTAACACATAGTGGTGAAGC
ATTGTTATTTAACTCTTGCATTTTTTCTTTAAACTAAACAACTTACAACT
TACTTATAAATTAGAGTAGTCGTAGTTCATAATTTCTAATCCTGTCTTCA
TATACCGATTGGTTATTTTGTAATAATAAATATTTTTCACAGGCACTGGA
TCAAACAACTTCCAATCTCCTTCAAAACAGCAACAACAGTCTCCCTCTGG
TTTTCCTGCCCCGACTTTTGCAGCCTCTCAAGGCAATCCTGGACAAGTAA
GATTCTTGACAATATTTAATGTTTTTTATCTATAAAAAGTTATTKCMCTT
CCTCTCCATTTAGCCCCGTCCATCAAGCAACAATGAATTCAATTCTCCCA
ATAACAATAGACAGCAACCCTCTGGAAGTTTTCCATCCCAAAATCATAAT
GGACAAGTAAGTGGCAATACGTAAAGTGAGTTTCAATCCTAAATCTGTTC
TTTTTTCAGCTTCAACCTCCAGCAAGTGGATTTTCCCCTCCCAACCAAAA
CAGACAAGTGAGTTAATTGAATCATAAGTAGGTCAATTGAGAACCTAATC
CTGTATTTTACAGCCTTCACAGCCCAATAATGGATACTTATCTCCAGGTA
GCGGTAATAACAACAGACAGGTAATTTGATATTGTCAAATACAATTCACA
TTCATAAAACTTAACTTATTTCTTTATCATCTACAGCCATCAAGACCCAA
CAATAATGGATTCCCATCCTCGAATAACAATAACAGACAAGTATGTTTTT
TTTTTTTAATCAGGTTGAGGGATTTTATTCCTAGCTTATAATCTTATTTT
TAGCCATCTGGAACAAACAATGGAGTCTCTTCTCCATCAAGAGGACAGGT
AATATCCATTATTCTGTATTTAGAATTGTGTTTTCGAATTAATACATCTA
TATATGTNAAGTAAGTTTGAAACTCATTTATTTACAAGGGGTCGATTAGA
TAAACTATTTCTATTTTCAATTTTATCAAAACTTTATTTAGATGAAATAT
TATTATTTGGTGGTGTGAAAGTAGATGTTTCAGAACATGTTCGTRCTCTC
ATATAAAAAAAGAAAAGATGAATAAGTAGCTCATTCTTTTTTTTTCTATC
CAGAACGTGTTTGATTACCGTAATGAGTACAGTTTATTACCTCAATAATC
ATTAGAAATAGGTTGACAAGGATGTTGGGAAAGTGTATAAGCCAAGGTGT
GATAAAACAACCTTGGTTACTGATCTAAAACAACAAATCCGGTCAAATCC
TATAATCATTTCCTTATCCTGTACAAAGAACGTAATTAAAGGTCTTTAAC
CATCTCCAAAAACATTGTTTTTGATATAAGAATAAACGCTTTAAAATGAA
CCACTTAATTAAAAAAAACAATACAGATATGATTTACTGTATGTTGATCT
TTTTGAACGTGTATTGAGTGAGTGATTGCTGTTATAGGTGATCATATTCA
ATAGGTGCATCTTTGCAACGTTAAAAAAAAGTTAAAAAAAAATATTATTC
TCAAAATTTATAGCAAATGACATTTGTATTCTTCCTTGAGTTGTTGTTCA
TTTCTATTAACAAATTATTAGTATTAACCCCTTGGAAGCGCTGCCAATAG
CTCTTACAGGATACAAAATTGATCGTCATAATATAAAATAAAATATGTAT
TTGAAAGGGGTCATACGGCGGAAACCAATGTAAGTCTCACACACCAAATA
AAAGTTTTAAACTATATCTAAACTTCTTAAGTATCTTAACTCATCCCCAA
AATTTGAAAACAAAGTTAGTAACGGATTTTATATGGCTAAGAAATATTAG
GGTGTATGTACCTAATAATTAAGTGATAAATGTATTTTTTTTTTTTTCGT
GATTTTTATTTAAGATTTGTTTTTTTATCGTTGATTTTTCTTTCCATTAT
TTGATCTTGATACTGTTTTAAATATGTTTTTTTAAATAGGTCGGGGGGGG
GGGGGGTGTWTTTAATCGACTCTGAATGTTTTCCTACATCCACGAACCCC
TCTCAAAGTAGATGTCTGAAGAATCATAAGCATATTTCATCATATTTATT
TTACACTTTTATCTTCTTTCCTTTTTAATCATCTAGCCTGGAAATGGTTT
TCCCAATCCCAATAAATTTGGACAAGTAATACGTCGTTGTTTTAAATTTT
ATGTATGACAAATAACATTTTTCTTTTTTTCCGATAGAATCCTCAATCTG
GATCAAATAACAGATTTCCTTCATCAGGCAATAATAATAATATAGGCGTA
TGTTTATTTCATCAATTTAACACTTGTATTGATAGTAATATCATATAACT
TTTTTTTGTTATAGGGACAACCAGGCAGACCTACTGGACAAAGGGTAAGT
CCCTCACTCCAGCTTTTGAGCCATTCTAATTACTATTTATTATCTATAGC
CCTCTGGTTCTAACAATAACTTTGCCAATSCAGCCACAAAGCCTTACAAA
AGCTGTCCCTCGGCCATGAAATGCGTTCCTAAGCAAAACTGCGACTTTAA
TGGTGTTATGGTCAATGAAATCATTACAACAGATCCTAAGTTTGATGACA
TCCGCGTTCCTCTTATTGTAAGTCATACACTTTATGTCCTTTAGGTCAGT
GACCCCTAACCTTTTTCTACCGGCGGACTGGTTAACACTTCACAATTTAC
TACAGACTGGAAGGGTTAGAAATAACCACTGTATTTTTTTATATATGCTG
CAAATCATTTTTGAAGGCCGGTCTCTGCATATTGTGCAGAGAGGACTTGG
TGTGTAGTAATTACCTGTTGTGATAAATCCATAGTTAAACTAGAATTCCT
GGTATTGTCTTCTAACAACTTTCTTTTTCTTGGAAGTCGTAACCAGCTTT
TTTCTTTCTTCATTGTGTATTTTCCACTTTGCAAGTGATGGAGAACGGTC
ATTTGAAAATAAAAGACCGCTCGAATAAGACTAGGACTTTAATAGAGGGT
GACTTACTACTCTATCTACAGCTGGTATGTTAAGAAGAAAACATTAATTA
TGAATGCAAATAGCAATAGAGTGCCGACAAAATTTAGTAGGTATTGAAGC
TGGAGCAATGGAGCAAAATKCTCTCTWCTTTTAATGTGCKCTTTTTTTTT
TCTTCATTTTTTTTTTTTTTTTGACCGCTTAAATGTTATTTTTTTATTAG
TAGAACAGCTATAGAATAAAAAAGGGAATTGAATTGACCCAATTGTGTAA
TGATTATCTTAAAAAATAAAAGAATTTTTTTTATTTGATAACATAAAATA
AAAAGATCAATTAAGAAAAATATTTTAATTCCACAAGATATTCATTACAC
GATTGTTTTAATTAAAGTACTTATTTCCTTTCATAATTATTTTAGTCTAA
TAAAAGCAGCCCTCTTCTGGTCAACAAAAAAAAAAGAAGAAGAAGATGCA
CGCTCCCTGAATAGATAGAAAAGCATATAGGAGACAAGGATTGTGCTAGG
ATTTACCATTAAGTTCACCTAATAATGAATTATTACAATTTGAATATAGT
CTGCAATACCCTATTTAGTGGTTTGGATTTAATCAGTCCTTGTTATATCT
TTTTTTTTAAATCCATACTCTTTTAGCAATACAAAATATTAGCATGGGCT
CTGACAGTCTCGAATACTCCGGATTCAAACAGACCAATATATCAACAAAC
CAATTACCTGAAATGTTTGGAGTATTATTGAACTATAATAGATAACAGGG
GTGTCTGCAGGGGGTGGCCTGGAGGTGCTGTAACCACCCTCCAAATCAAG
TATTTTTTTTATTTATCCGACAAAAATGATAGCTTTGTGTGAAATCTTTA
AAATGTGAATTTTTTTAATAGAAATTAAAGTCGTTTGGGGGTCCAATTTA
GTCCCGGATCAAATTTTGAAAAATTAGCCCCCCCCCCCCTTAAAAATATT
ATGCCCTTGTTAAATGTTATATCCCCTTTTCCGACGTTGAATTTTTGTCC
CAGACATATAATTTTTTTACTTATGCTGCCCAGTTACGCCAAACAGCCAA
ATGGAACGCCTTTCGATTTATTTACTCTATAATATGACTTAATTTTATGT
CACGTATTTTTTTTTTTATATTTATAGCCATGTGTGAATCAAAACAGTGG
AACCATTGATGTATGTTGCCGTGATCCAAATTACAAGGKCCCCTGGCCAC
AAAACAACTGA
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