EMLSAG00000004991, EMLSAG00000004991-687757 (gene) Lepeophtheirus salmonis
|
Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of EMLSAG00000004991 vs. GO
Match: - (symbol:HUWE1 "E3 ubiquitin-protein ligase HUWE1" species:9606 "Homo sapiens" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] Pfam:PF00632 InterPro:IPR000569 PROSITE:PS50237 SMART:SM00119 GO:GO:0016567 GO:GO:0004842 SUPFAM:SSF56204 EMBL:Z97054 EMBL:AL592046 EMBL:Z94044 HGNC:HGNC:30892 ChiTaRS:HUWE1 ProteinModelPortal:H0Y659 Ensembl:ENST00000426907 OrthoDB:EOG7G7KN8 NextBio:35519839 Uniprot:H0Y659) HSP 1 Score: 874.004 bits (2257), Expect = 0.000e+0 Identity = 457/786 (58.14%), Postives = 548/786 (69.72%), Query Frame = 0
Query: 2257 ISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNFEHDRSG------------------KDKGGLLDRF-TKETVVLNAPSK----------------------------------QXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTI----------ADQTNEENKMDVDENS----------SDSTSEAKANSQWK---IESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQL----------ADNKEME---SSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
++ + L+L+V+VLTS SCSEEGLED +LL LS G TRD +L+LLL GA+ LG + + L EL + N E R K KG + RF E VV+ A K Q + D + A K + + S + ++ A+ ++ I A EE+ MDVD+ S SD T + + + + + LSEQLSLD LW+ LG+CLK+L E+ D HAVLVLQ +VEAFFLVHA T+ + K V ++TR+ QLAHI+++ A ++ S +S S+SL PDT+KFL FAETHRTVLNQILRQST HLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE LR+ED+AVHVRR++VFE+S+REL R+SPEE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 414 LTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAEL---REYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKRPLGGRELQLPSMSMLTSKTSTQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMSESSQSEASVRREESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHA--TERESKPPV--RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 1192 HSP 2 Score: 93.9745 bits (232), Expect = 2.182e-17 Identity = 38/56 (67.86%), Postives = 51/56 (91.07%), Query Frame = 0
Query: 2111 DNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
D+E LSCLL+LLFVD+ K+NT+RLHR+LRNLCYHA TR WV+R L+SIL+R++E++
Sbjct: 20 DHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE 75
BLAST of EMLSAG00000004991 vs. GO
Match: - (symbol:HUWE1 "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] Pfam:PF00632 InterPro:IPR000449 InterPro:IPR000569 InterPro:IPR009060 InterPro:IPR016024 Pfam:PF00627 PROSITE:PS50237 SMART:SM00119 SUPFAM:SSF48371 GO:GO:0016567 InterPro:IPR015940 SMART:SM00165 SUPFAM:SSF46934 GO:GO:0004842 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 PROSITE:PS50030 InterPro:IPR004170 Pfam:PF02825 PROSITE:PS50918 OMA:GMTQEVG InterPro:IPR025527 InterPro:IPR010309 InterPro:IPR010314 Pfam:PF14377 Pfam:PF06012 Pfam:PF06025 EMBL:AAEX03026386 EMBL:AAEX03026387 Ensembl:ENSCAFT00000025916 Uniprot:F6XKL7) HSP 1 Score: 882.093 bits (2278), Expect = 0.000e+0 Identity = 457/774 (59.04%), Postives = 549/774 (70.93%), Query Frame = 0
Query: 2257 ISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDL---------------QNELLSIKSTNFEHDRSGKDKGGLLDRF-TKETVVLNAPSK----------QXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTI-------------------------ADQTNEENKMDVDENS----------SDSTSEAKANSQWK---IESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQL----------ADNKEME---SSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
++ + L+L+V+VLTS SCSEEGLED +LL LS G TRD +L+LLL GA+ LG + + L Q E LS E ++ K KG + RF E VV+ A K Q + D + A K + + S + ++ A+ ++ I A EE+ MDVD+ S SD T + + + + + LSEQLSLD LW+ LG+CLK+L E+ D HAVLVLQ +VEAFFLVHA T+ + K V ++TR+ QLAHI+++ A ++ S +S S+SL PDT+KFL FAETHRTVLNQILRQST HLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE LR+ED+AVHVRR++VFE+S+REL R+SPEE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 3622 LTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDPATRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKFFLRVLQVIIQLRDDTRRANKKAKQTGRLGSSGLGSASSIQAAVRQLEAEADAIIQMVREGQRARRQQQAATSESSQSEASVRREESPMDVDQPSPSAQDTQSIGSDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHA--TERESKPPV--RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 4391 HSP 2 Score: 382.489 bits (981), Expect = 1.182e-104 Identity = 254/656 (38.72%), Postives = 368/656 (56.10%), Query Frame = 0
Query: 397 DMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTE--GNNEEDSQQQSGKEEEENEGGSFG-------LSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSP-IEEDVFRTT---ESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCS--GSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVC---SRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK-----SKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPN--------------EEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIR---TSTLNSQTT--------SKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETD---EDTRL-------LVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV--KTPDICESSN 991
DMGF+RE +EA+ T M +ATEYLLT +R +S E++ +MRAIAMSL D+ Q + + E +E+ ++++ +++EE E L + + FT LPGC +L+D LP+TV+R CDL++ RNG ++++ IL+ ++ +V A DVL+ L L S + + E + + +++ A RI L LLFEE KL CA +VE S ++ ++ LL V L+ V TPKW+TP+LL ID YEK + RRA +T + W+W+D SGRWC Y+ ++N TID A+ SGETSVR T GRR+YT+QF TM+Q NEETGNRRP+M+++ +K S+++ + + + M++ +E K +D EE I I +GL D + L++ACV + +PVD D L+A LRLCLRLT++ + A+ FA L +++L L QSS F+G T L TLL+RH++EDP TLRHTMEKV+R TS S T+ S+EINYILRVL PAACR+P IF VA +R P+P +D E+ R+ LVK+ P K S P + + + VI D+LN L P+ E S+
Sbjct: 1372 DMGFTREHAMEALLNTSTMEQATEYLLTHPPPI------------MGGVVRDLSMS-EEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNNNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKANDTPLALENTNTEKETSLEETKIGEI-LIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKP--SPLPVIPDTIKEVIYDMLNALAAYHAPEEAEKSD 2011 HSP 3 Score: 363.999 bits (933), Expect = 5.099e-99 Identity = 171/283 (60.42%), Postives = 206/283 (72.79%), Query Frame = 0
Query: 12 TTIEKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNL---SIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSK---GSKGEDDKNSGGGA 288
T + K +K P + +IKQIKPLL ASSRLGRALAELF LLVKLCVGSP+RQRR H T AP+ A + ASAL +LL GLSW PPP +P P FRLTFF CSVGFT PMLFD++KYPYHLML KF+ SGG A F+TF WALS GGK+ EGLE ++LPDGTGEFLD+WLML+E+MVNP VLESPH+LP + NF F +++L+ T K AF + +W + PLKVYG RMAES+L+ILCHIL+GE +I E+LSK GS+GE+D G
Sbjct: 1073 TASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVAEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGG 1355 HSP 4 Score: 297.36 bits (760), Expect = 7.878e-79 Identity = 259/764 (33.90%), Postives = 369/764 (48.30%), Query Frame = 0
Query: 1467 GNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFR-----RSGAPLMM-----DVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE---------NTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAAT-----TALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVP-------PLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRN--MDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQP---------------RESEEAI-GGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS------NVPEVNPEFLAALPPNIQEEVLAQQRMEQQR-QLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNS-LSSILRGSV--SRL----GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
G R+ + R L G+++ ++ N + P+ P ILQ LG S +++ R A L++ ++ S + +D+ D +AT +G LSSIP+AL RW EE +V+D ESMHDCV+ +K I+ +E ++ EEE KI K D N D+ T + T + ++ TP+ P TTPS+T AAT T +T S+ A+ ++ Q+L + +++P P S TT + S + S + + ++SQ MDT++ A T L+ A +S T S+T VT S GD S E QP R+S AI G S P+PSTS ++P L + +PEGVDPSFLAALP+++R+EV+ +Q ++ + A N+ V EV+PEFLAALPP IQEEVLAQQR EQQR +L+Q + + P+DP F QTLP+ LR+SVL DMEDS +A +P ++AAEAQ+LRRE E R R MHER F + S LS+ILR SRL G QY + F++ GS + R + + +R +GR LLD+E LSCLL+LLFVD+ K+NT+RLHR+LRNLCYHA TR WV+R L+SIL+R++E++
Sbjct: 2587 GIGRSQRTLRQLTANTGHTIHVHYPGNRQ----PNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAG---TLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKTNDSAEQN--------LSDGTPMPDSYP---------------------TTPSSTDAATSESKDTLVTLQPSQQQQTLPPPPALGEIPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCT---------------LEEAVGDTSAAGSSEQPTAGSSTPGDAPTVVTEVQSR---GDGSGEPTQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDP-----LAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSANSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSS---------SHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE 3282
BLAST of EMLSAG00000004991 vs. GO
Match: - (symbol:HUWE1 "E3 ubiquitin-protein ligase HUWE1" species:9606 "Homo sapiens" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] Pfam:PF00632 InterPro:IPR000449 InterPro:IPR000569 InterPro:IPR009060 Pfam:PF00627 PROSITE:PS50237 SMART:SM00119 GO:GO:0005634 GO:GO:0005737 GO:GO:0016567 InterPro:IPR015940 SMART:SM00165 SUPFAM:SSF46934 GO:GO:0004842 SUPFAM:SSF56204 PROSITE:PS50030 InterPro:IPR004170 Pfam:PF02825 PROSITE:PS50918 EMBL:Z97054 EMBL:AL592046 EMBL:Z94044 HGNC:HGNC:30892 ChiTaRS:HUWE1 InterPro:IPR025527 Pfam:PF14377 ProteinModelPortal:H0Y5W0 PRIDE:H0Y5W0 Ensembl:ENST00000427052 NextBio:35519783 Bgee:H0Y5W0 Uniprot:H0Y5W0) HSP 1 Score: 841.647 bits (2173), Expect = 0.000e+0 Identity = 401/568 (70.60%), Postives = 467/568 (82.22%), Query Frame = 0
Query: 2412 EENKMDVDENS----------SDSTSEAKANSQWK---IESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQL----------ADNKEME---SSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
EE+ MDVD+ S SD T + + + + + LSEQLSLD LW+ LG+CLK+L E+ D HAVLVLQ +VEAFFLVHA T+ + K V ++TR+ QLAHI+++ A ++ S +S S+SL PDT+KFL FAETHRTVLNQILRQST HLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE LR+ED+AVHVRR++VFE+S+REL R+SPEE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 2840 EESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHA--TERESKPPV--RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 3403 HSP 2 Score: 563.148 bits (1450), Expect = 8.282e-160 Identity = 411/1081 (38.02%), Postives = 587/1081 (54.30%), Query Frame = 0
Query: 397 DMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQED-LMRAIAMSLDVDVVSFQVSSNTE--GNNEEDSQQQSGKEEEENEGGSFG-------LSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSP-IEEDVFRTT---ESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCS--GSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVC---SRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK-----SKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPN--------------EEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIR---TSTLNSQTT--------SKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETD---EDTRL-------LVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV--KTPDICESSN--NATVVESATELKNNKGGGAPNVIRNMX--TGDL---PGVLXXPPVTITTAQPCDTSA-------------SKDMK-------------------KPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELL---TSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSARKSKNDVPDSSA----EDSTSN--NPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERD-GSDNFTII 1366
DMGF+RE +EA+ T M +ATEYLLT +R LS+ +ED +MRAIAMSL D+ Q + + E +E+ ++++ +++EE E L + + FT LPGC +L+D LP+TV+R CDL++ RNG ++++ IL+ ++ +V A DVL+ L L S + + E + + +++ A RI L LLFEE KL CA +VE S ++ ++ LL V L+ V TPKW+TP+LL ID YEK + RRA +T S W+W+D SGRWC Y+ ++N TID A+ SGETSVR T GRR+YT+QF TM+Q NEETGNRRP+M+++ +K S+++ + + + M++ +E K +D EE I I +GL D + L++ACV + +PVD D L+A LRLCLRLT++ + A+ FA L +++L L QSS F+G T L TLL+RH++EDP TLRHTMEKV+R TS S T+ S+EINYILRVL PAACR+P IF VA +R P+P +D E+ R+ LVK+ P K S P + + + VI D+LN L P+ + S+ + + +L + G R++ + D G V +TSA S+D K KP + S + RLLAELV+SY G A LI ++ Y G E IKED S LAF+LD LL + DKD L + +A+LA+ +AQ ALV EVK AL R+L ES KH+++QA+ C+IST++ESCPS+ A + MNNI+++ LK+GL+ DLARVPH+LDLSSPN+A T+N+ALKPLETLSRIVNQP++L S S + + A +DS+SN +P G + ++ E + +V T +E A GD D EA E D A + S ++Q + + ++++D+LLERD GS N TII
Sbjct: 361 DMGFTREHAMEALLNTSTMEQATEYLLTHPPPI------------MGGVVRD--LSMSEEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEI-LIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKP--SPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPST-SSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEA----EVQEEDHDV------TQTEV--ADGDIM---DGEA-ETDSVVIAGQPEVLSSQEMQV-----ENELEDLIDELLERDGGSGNSTII 1402 HSP 3 Score: 365.54 bits (937), Expect = 1.378e-99 Identity = 171/283 (60.42%), Postives = 206/283 (72.79%), Query Frame = 0
Query: 12 TTIEKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNL---SIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSK---GSKGEDDKNSGGGA 288
T + K +K P + +IKQIKPLL ASSRLGRALAELF LLVKLCVGSP+RQRR H T AP+ A + ASAL +LL GLSW PPP +P P FRLTFF CSVGFT PMLFD++KYPYHLML KF+ SGG A F+TF WALS GGK+ EGLE ++LPDGTGEFLD+WLML+E+MVNP VLESPH+LP + NF F +++L+ T K AF + +W + PLKVYG RMAES+L+ILCHIL+GE +I E+LSK GS+GE+D G
Sbjct: 62 TASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGG 344 HSP 4 Score: 291.967 bits (746), Expect = 2.846e-77 Identity = 260/764 (34.03%), Postives = 368/764 (48.17%), Query Frame = 0
Query: 1467 GNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFR-----RSGAPLMM-----DVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE---------NTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAAT-----TALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVP-------PLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRN--MDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQP---------------RESEEAI-GGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS------NVPEVNPEFLAALPPNIQEEVLAQQRMEQQR-QLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNS-LSSILRGSV--SRL----GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
G R+ + R L G+++ ++ N P+ P ILQ LG S +++ R A L++ ++ S + +D+ D +AT +G LSSIP+AL RW EE +V+D ESMHDCV+ +K I+ +E ++ EEE KI K D N D+ A T + + TE G TP+ P TTPS+T AAT T T SS+ A+ ++ Q+L + +++P P S TT + S + S + + ++SQ MDT++ A T L+ A +S S+T +V + G S ES QP R+S AI G S P+PSTS ++P L + +PEGVDPSFLAALP+++R+EV+ +Q ++ + A N+ V EV+PEFLAALPP IQEEVLAQQR EQQR +L+Q + + P+DP F QTLP+ LR+SVL DMEDS +A +P ++AAEAQ+LRRE E R R MHER F + S LS+ILR SRL G QY + F++ GS + R + + +R +GR LLD+E LSCLL+LLFVD+ K+NT+RLHR+LRNLCYHA TR WV+R L+SIL+R++E++
Sbjct: 1576 GIGRSQRTLRQLTANTGHTIHVHYPGN----RQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAG---TLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQS-AQCTASKSNDSTEQNLSDG-------TPMPDSYP---------------------TTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCT---------------LEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDG---SGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDP-----LAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSS---------SHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE 2271 HSP 5 Score: 68.5514 bits (166), Expect = 1.530e-9 Identity = 48/123 (39.02%), Postives = 65/123 (52.85%), Query Frame = 0
Query: 2257 ISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDL---------------QNELLSIKSTNFEHDRSGKDKGGLLDRF-TKETVVLNAPSKQ 2363
++ + L+L+V+VLTS SCSEEGLED +LL LS G TRD +L+LLL GA+ LG + + L Q E LS E ++ K KG + RF E VV+ A K+
Sbjct: 2610 LTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKR 2732
BLAST of EMLSAG00000004991 vs. GO
Match: - (symbol:HUWE1 "E3 ubiquitin-protein ligase HUWE1" species:9606 "Homo sapiens" [GO:0000209 "protein polyubiquitination" evidence=IDA] [GO:0003677 "DNA binding" evidence=ISS] [GO:0004842 "ubiquitin-protein ligase activity" evidence=IDA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005634 "nucleus" evidence=ISS;IDA] [GO:0005737 "cytoplasm" evidence=IDA] [GO:0006284 "base-excision repair" evidence=IMP] [GO:0006513 "protein monoubiquitination" evidence=IDA] [GO:0016574 "histone ubiquitination" evidence=IDA] [GO:0030154 "cell differentiation" evidence=IEA] [GO:0042787 "protein ubiquitination involved in ubiquitin-dependent protein catabolic process" evidence=IBA] [GO:0044822 "poly(A) RNA binding" evidence=IDA] [GO:0070062 "extracellular vesicular exosome" evidence=IDA] [GO:0005730 "nucleolus" evidence=IDA] Pfam:PF00632 InterPro:IPR000449 InterPro:IPR000569 InterPro:IPR009060 InterPro:IPR016024 Pfam:PF00627 PROSITE:PS50237 SMART:SM00119 UniPathway:UPA00143 GO:GO:0005634 GO:GO:0005737 GO:GO:0070062 Reactome:REACT_6900 GO:GO:0030154 SUPFAM:SSF48371 GO:GO:0006284 GO:GO:0003677 InterPro:IPR015940 SMART:SM00165 SUPFAM:SSF46934 GO:GO:0004842 GO:GO:0000209 eggNOG:COG5021 TreeFam:TF323417 GO:GO:0042787 SUPFAM:SSF56204 GO:GO:0016574 GO:GO:0006513 PROSITE:PS50030 InterPro:IPR004170 Pfam:PF02825 PROSITE:PS50918 EMBL:Z97054 MIM:300705 EMBL:AY772009 EMBL:DQ097177 EMBL:AY929612 EMBL:AL592046 EMBL:Z94044 EMBL:AB071605 EMBL:AB002310 EMBL:AB046798 EMBL:AF161390 EMBL:BC002602 EMBL:BC063505 EMBL:AF057569 EMBL:CR456813 EMBL:AL162050 PIR:T47165 RefSeq:NP_113584.3 RefSeq:XP_005262022.1 RefSeq:XP_005262023.1 RefSeq:XP_005262024.1 RefSeq:XP_005262027.1 RefSeq:XP_005278018.1 RefSeq:XP_005278019.1 RefSeq:XP_005278020.1 RefSeq:XP_005278023.1 UniGene:Hs.136905 PDB:2EKK PDB:3G1N PDB:3H1D PDBsum:2EKK PDBsum:3G1N PDBsum:3H1D ProteinModelPortal:Q7Z6Z7 SMR:Q7Z6Z7 BioGrid:115385 DIP:DIP-34362N IntAct:Q7Z6Z7 MINT:MINT-1576525 PhosphoSite:Q7Z6Z7 DMDM:73915353 PaxDb:Q7Z6Z7 PRIDE:Q7Z6Z7 Ensembl:ENST00000262854 Ensembl:ENST00000342160 GeneID:10075 KEGG:hsa:10075 UCSC:uc004dsn.4 UCSC:uc004dsp.4 CTD:10075 GeneCards:GC0XM053575 HGNC:HGNC:30892 HPA:CAB022718 HPA:HPA002548 MIM:300697 MIM:300706 neXtProt:NX_Q7Z6Z7 Orphanet:85328 PharmGKB:PA128394567 HOVERGEN:HBG080254 KO:K10592 OMA:GMTQEVG ChiTaRS:HUWE1 EvolutionaryTrace:Q7Z6Z7 GeneWiki:HUWE1 GenomeRNAi:10075 NextBio:38085 PRO:PR:Q7Z6Z7 ArrayExpress:Q7Z6Z7 Bgee:Q7Z6Z7 Genevestigator:Q7Z6Z7 InterPro:IPR025527 InterPro:IPR010309 InterPro:IPR010314 Pfam:PF14377 Pfam:PF06012 Pfam:PF06025 Uniprot:Q7Z6Z7) HSP 1 Score: 838.951 bits (2166), Expect = 0.000e+0 Identity = 401/568 (70.60%), Postives = 467/568 (82.22%), Query Frame = 0
Query: 2412 EENKMDVDENS----------SDSTSEAKANSQWK---IESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQL----------ADNKEME---SSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
EE+ MDVD+ S SD T + + + + + LSEQLSLD LW+ LG+CLK+L E+ D HAVLVLQ +VEAFFLVHA T+ + K V ++TR+ QLAHI+++ A ++ S +S S+SL PDT+KFL FAETHRTVLNQILRQST HLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE LR+ED+AVHVRR++VFE+S+REL R+SPEE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 3806 EESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHA--TERESKPPV--RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 4369 HSP 2 Score: 560.451 bits (1443), Expect = 8.199e-159 Identity = 409/1080 (37.87%), Postives = 586/1080 (54.26%), Query Frame = 0
Query: 397 DMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTE--GNNEEDSQQQSGKEEEENEGGSFG-------LSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSP-IEEDVFRTT---ESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCS--GSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVC---SRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK-----SKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPN--------------EEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIR---TSTLNSQTT--------SKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETD---EDTRL-------LVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV--KTPDICESSN--NATVVESATELKNNKGGGAPNVIRNMX--TGDL---PGVLXXPPVTITTAQPCDTSA-------------SKDMKK-------------------PFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELL---TSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSARKSKNDVPDSSA----EDSTSN--NPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERD-GSDNFTII 1366
DMGF+RE +EA+ T M +ATEYLLT +R +S E++ +MRAIAMSL D+ Q + + E +E+ ++++ +++EE E L + + FT LPGC +L+D LP+TV+R CDL++ RNG ++++ IL+ ++ +V A DVL+ L L S + + E + + +++ A RI L LLFEE KL CA +VE S ++ ++ LL V L+ V TPKW+TP+LL ID YEK + RRA +T S W+W+D SGRWC Y+ ++N TID A+ SGETSVR T GRR+YT+QF TM+Q NEETGNRRP+M+++ +K S+++ + + + M++ +E K +D EE I I +GL D + L++ACV + +PVD D L+A LRLCLRLT++ + A+ FA L +++L L QSS F+G T L TLL+RH++EDP TLRHTMEKV+R TS S T+ S+EINYILRVL PAACR+P IF VA +R P+P +D E+ R+ LVK+ P K S P + + + VI D+LN L P+ + S+ + + +L + G R++ + D G V +TSA S+D KK P + S + RLLAELV+SY G A LI ++ Y G E IKED S LAF+LD LL + DKD L + +A+LA+ +AQ ALV EVK AL R+L ES KH+++QA+ C+IST++ESCPS+ A + MNNI+++ LK+GL+ DLARVPH+LDLSSPN+A T+N+ALKPLETLSRIVNQP++L S S + + A +DS+SN +P G + ++ E + +V T +E A GD D EA E D A + S ++Q + + ++++D+LLERD GS N TII
Sbjct: 1327 DMGFTREHAMEALLNTSTMEQATEYLLTHPPPI------------MGGVVRDLSMS-EEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEI-LIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKP--SPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPST-SSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEA----EVQEEDHDV------TQTEV--ADGDIM---DGEA-ETDSVVIAGQPEVLSSQEMQV-----ENELEDLIDELLERDGGSGNSTII 2368 HSP 3 Score: 364.385 bits (934), Expect = 4.480e-99 Identity = 171/283 (60.42%), Postives = 206/283 (72.79%), Query Frame = 0
Query: 12 TTIEKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNL---SIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSK---GSKGEDDKNSGGGA 288
T + K +K P + +IKQIKPLL ASSRLGRALAELF LLVKLCVGSP+RQRR H T AP+ A + ASAL +LL GLSW PPP +P P FRLTFF CSVGFT PMLFD++KYPYHLML KF+ SGG A F+TF WALS GGK+ EGLE ++LPDGTGEFLD+WLML+E+MVNP VLESPH+LP + NF F +++L+ T K AF + +W + PLKVYG RMAES+L+ILCHIL+GE +I E+LSK GS+GE+D G
Sbjct: 1028 TASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGG 1310 HSP 4 Score: 292.352 bits (747), Expect = 2.629e-77 Identity = 260/764 (34.03%), Postives = 368/764 (48.17%), Query Frame = 0
Query: 1467 GNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFR-----RSGAPLMM-----DVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE---------NTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAAT-----TALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVP-------PLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRN--MDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQP---------------RESEEAI-GGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS------NVPEVNPEFLAALPPNIQEEVLAQQRMEQQR-QLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNS-LSSILRGSV--SRL----GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
G R+ + R L G+++ ++ N P+ P ILQ LG S +++ R A L++ ++ S + +D+ D +AT +G LSSIP+AL RW EE +V+D ESMHDCV+ +K I+ +E ++ EEE KI K D N D+ A T + + TE G TP+ P TTPS+T AAT T T SS+ A+ ++ Q+L + +++P P S TT + S + S + + ++SQ MDT++ A T L+ A +S S+T +V + G S ES QP R+S AI G S P+PSTS ++P L + +PEGVDPSFLAALP+++R+EV+ +Q ++ + A N+ V EV+PEFLAALPP IQEEVLAQQR EQQR +L+Q + + P+DP F QTLP+ LR+SVL DMEDS +A +P ++AAEAQ+LRRE E R R MHER F + S LS+ILR SRL G QY + F++ GS + R + + +R +GR LLD+E LSCLL+LLFVD+ K+NT+RLHR+LRNLCYHA TR WV+R L+SIL+R++E++
Sbjct: 2542 GIGRSQRTLRQLTANTGHTIHVHYPGN----RQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAG---TLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQS-AQCTASKSNDSTEQNLSDG-------TPMPDSYP---------------------TTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCT---------------LEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDG---SGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDP-----LAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSS---------SHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE 3237 HSP 5 Score: 67.781 bits (164), Expect = 2.220e-9 Identity = 48/123 (39.02%), Postives = 65/123 (52.85%), Query Frame = 0
Query: 2257 ISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDL---------------QNELLSIKSTNFEHDRSGKDKGGLLDRF-TKETVVLNAPSKQ 2363
++ + L+L+V+VLTS SCSEEGLED +LL LS G TRD +L+LLL GA+ LG + + L Q E LS E ++ K KG + RF E VV+ A K+
Sbjct: 3576 LTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKR 3698
BLAST of EMLSAG00000004991 vs. GO
Match: - (symbol:HUWE1 "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0000209 "protein polyubiquitination" evidence=IEA] [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0005737 "cytoplasm" evidence=IEA] [GO:0006284 "base-excision repair" evidence=IEA] [GO:0006513 "protein monoubiquitination" evidence=IEA] [GO:0016574 "histone ubiquitination" evidence=IEA] Pfam:PF00632 InterPro:IPR000449 InterPro:IPR000569 InterPro:IPR009060 InterPro:IPR016024 Pfam:PF00627 PROSITE:PS50237 SMART:SM00119 GO:GO:0005737 SUPFAM:SSF48371 GO:GO:0006284 InterPro:IPR015940 SMART:SM00165 SUPFAM:SSF46934 GO:GO:0004842 GO:GO:0000209 TreeFam:TF323417 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 GO:GO:0016574 GO:GO:0006513 PROSITE:PS50030 InterPro:IPR004170 Pfam:PF02825 PROSITE:PS50918 InterPro:IPR025527 InterPro:IPR010309 InterPro:IPR010314 Pfam:PF14377 Pfam:PF06012 Pfam:PF06025 EMBL:AAEX03026386 EMBL:AAEX03026387 Ensembl:ENSCAFT00000025893 Uniprot:F6XM35) HSP 1 Score: 838.951 bits (2166), Expect = 0.000e+0 Identity = 401/568 (70.60%), Postives = 467/568 (82.22%), Query Frame = 0
Query: 2412 EENKMDVDENS----------SDSTSEAKANSQWK---IESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQL----------ADNKEME---SSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
EE+ MDVD+ S SD T + + + + + LSEQLSLD LW+ LG+CLK+L E+ D HAVLVLQ +VEAFFLVHA T+ + K V ++TR+ QLAHI+++ A ++ S +S S+SL PDT+KFL FAETHRTVLNQILRQST HLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE LR+ED+AVHVRR++VFE+S+REL R+SPEE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 3807 EESPMDVDQPSPSAQDTQSIGSDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHA--TERESKPPV--RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 4370 HSP 2 Score: 382.104 bits (980), Expect = 1.555e-104 Identity = 254/656 (38.72%), Postives = 368/656 (56.10%), Query Frame = 0
Query: 397 DMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTE--GNNEEDSQQQSGKEEEENEGGSFG-------LSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSP-IEEDVFRTT---ESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCS--GSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVC---SRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK-----SKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPN--------------EEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIR---TSTLNSQTT--------SKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETD---EDTRL-------LVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV--KTPDICESSN 991
DMGF+RE +EA+ T M +ATEYLLT +R +S E++ +MRAIAMSL D+ Q + + E +E+ ++++ +++EE E L + + FT LPGC +L+D LP+TV+R CDL++ RNG ++++ IL+ ++ +V A DVL+ L L S + + E + + +++ A RI L LLFEE KL CA +VE S ++ ++ LL V L+ V TPKW+TP+LL ID YEK + RRA +T + W+W+D SGRWC Y+ ++N TID A+ SGETSVR T GRR+YT+QF TM+Q NEETGNRRP+M+++ +K S+++ + + + M++ +E K +D EE I I +GL D + L++ACV + +PVD D L+A LRLCLRLT++ + A+ FA L +++L L QSS F+G T L TLL+RH++EDP TLRHTMEKV+R TS S T+ S+EINYILRVL PAACR+P IF VA +R P+P +D E+ R+ LVK+ P K S P + + + VI D+LN L P+ E S+
Sbjct: 1327 DMGFTREHAMEALLNTSTMEQATEYLLTHPPPI------------MGGVVRDLSMS-EEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNNNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKANDTPLALENTNTEKETSLEETKIGEI-LIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKP--SPLPVIPDTIKEVIYDMLNALAAYHAPEEAEKSD 1966 HSP 3 Score: 363.999 bits (933), Expect = 4.833e-99 Identity = 171/283 (60.42%), Postives = 206/283 (72.79%), Query Frame = 0
Query: 12 TTIEKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNL---SIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSK---GSKGEDDKNSGGGA 288
T + K +K P + +IKQIKPLL ASSRLGRALAELF LLVKLCVGSP+RQRR H T AP+ A + ASAL +LL GLSW PPP +P P FRLTFF CSVGFT PMLFD++KYPYHLML KF+ SGG A F+TF WALS GGK+ EGLE ++LPDGTGEFLD+WLML+E+MVNP VLESPH+LP + NF F +++L+ T K AF + +W + PLKVYG RMAES+L+ILCHIL+GE +I E+LSK GS+GE+D G
Sbjct: 1028 TASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVAEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGG 1310 HSP 4 Score: 296.975 bits (759), Expect = 1.037e-78 Identity = 259/764 (33.90%), Postives = 369/764 (48.30%), Query Frame = 0
Query: 1467 GNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFR-----RSGAPLMM-----DVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE---------NTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAAT-----TALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVP-------PLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRN--MDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQP---------------RESEEAI-GGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS------NVPEVNPEFLAALPPNIQEEVLAQQRMEQQR-QLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNS-LSSILRGSV--SRL----GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
G R+ + R L G+++ ++ N + P+ P ILQ LG S +++ R A L++ ++ S + +D+ D +AT +G LSSIP+AL RW EE +V+D ESMHDCV+ +K I+ +E ++ EEE KI K D N D+ T + T + ++ TP+ P TTPS+T AAT T +T S+ A+ ++ Q+L + +++P P S TT + S + S + + ++SQ MDT++ A T L+ A +S T S+T VT S GD S E QP R+S AI G S P+PSTS ++P L + +PEGVDPSFLAALP+++R+EV+ +Q ++ + A N+ V EV+PEFLAALPP IQEEVLAQQR EQQR +L+Q + + P+DP F QTLP+ LR+SVL DMEDS +A +P ++AAEAQ+LRRE E R R MHER F + S LS+ILR SRL G QY + F++ GS + R + + +R +GR LLD+E LSCLL+LLFVD+ K+NT+RLHR+LRNLCYHA TR WV+R L+SIL+R++E++
Sbjct: 2542 GIGRSQRTLRQLTANTGHTIHVHYPGNRQ----PNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAG---TLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQSAQCTASKTNDSAEQN--------LSDGTPMPDSYP---------------------TTPSSTDAATSESKDTLVTLQPSQQQQTLPPPPALGEIPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCT---------------LEEAVGDTSAAGSSEQPTAGSSTPGDAPTVVTEVQSR---GDGSGEPTQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDP-----LAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSANSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSS---------SHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE 3237 HSP 5 Score: 68.1662 bits (165), Expect = 1.862e-9 Identity = 48/123 (39.02%), Postives = 65/123 (52.85%), Query Frame = 0
Query: 2257 ISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDL---------------QNELLSIKSTNFEHDRSGKDKGGLLDRF-TKETVVLNAPSKQ 2363
++ + L+L+V+VLTS SCSEEGLED +LL LS G TRD +L+LLL GA+ LG + + L Q E LS E ++ K KG + RF E VV+ A K+
Sbjct: 3577 LTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDPATRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKR 3699
BLAST of EMLSAG00000004991 vs. GO
Match: - (symbol:HUWE1 "Uncharacterized protein" species:9913 "Bos taurus" [GO:0000209 "protein polyubiquitination" evidence=IEA] [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0005737 "cytoplasm" evidence=IEA] [GO:0006284 "base-excision repair" evidence=IEA] [GO:0006513 "protein monoubiquitination" evidence=IEA] [GO:0016574 "histone ubiquitination" evidence=IEA] Pfam:PF00632 InterPro:IPR000449 InterPro:IPR000569 InterPro:IPR009060 InterPro:IPR016024 Pfam:PF00627 PROSITE:PS50237 SMART:SM00119 GO:GO:0005737 SUPFAM:SSF48371 GO:GO:0006284 InterPro:IPR015940 SMART:SM00165 SUPFAM:SSF46934 GO:GO:0004842 GO:GO:0000209 TreeFam:TF323417 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 GO:GO:0016574 GO:GO:0006513 PROSITE:PS50030 InterPro:IPR004170 Pfam:PF02825 PROSITE:PS50918 CTD:10075 KO:K10592 OMA:GMTQEVG InterPro:IPR025527 InterPro:IPR010309 InterPro:IPR010314 Pfam:PF14377 Pfam:PF06012 Pfam:PF06025 OrthoDB:EOG7G7KN8 EMBL:DAAA02073284 EMBL:DAAA02073283 RefSeq:NP_001103474.1 UniGene:Bt.1460 PRIDE:E1BNY9 Ensembl:ENSBTAT00000008060 GeneID:511826 KEGG:bta:511826 NextBio:20870115 Uniprot:E1BNY9) HSP 1 Score: 838.565 bits (2165), Expect = 0.000e+0 Identity = 400/568 (70.42%), Postives = 467/568 (82.22%), Query Frame = 0
Query: 2412 EENKMDVDENS----------SDSTSEAKANSQWK---IESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQL----------ADNKEME---SSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
EE+ MDVD+ S SD T + + + + + LSEQL+LD LW+ LG+CLK+L E+ D HAVLVLQ +VEAFFLVHA T+ + K V ++TR+ QLAHI+++ A ++ S +S S+SL PDT+KFL FAETHRTVLNQILRQST HLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE LR+ED+AVHVRR++VFE+S+REL R+SPEE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 3807 EESPMDVDQPSPSAQDTQSIGSDGTPQGEKEKEERPPELPLLSEQLNLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHA--TERESKPPV--RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 4370 HSP 2 Score: 561.607 bits (1446), Expect = 3.483e-159 Identity = 409/1080 (37.87%), Postives = 585/1080 (54.17%), Query Frame = 0
Query: 397 DMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTE--GNNEEDSQQQSGKEEEENEGGSF-------GLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSP-IEEDVFRTT---ESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCS--GSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVC---SRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK-----SKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPN--------------EEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIR---TSTLNSQTT--------SKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETD---EDTRL-------LVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV--KTPDICESSN--NATVVESATELKNNKGGGAPNVIRNMX--TGDL---PGVLXXPPVTITTAQPCDTSA-------------SKDMKK-------------------PFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELL---TSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSARKSKNDVPD----SSAEDSTSN--NPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERD-GSDNFTII 1366
DMGF+RE +EA+ T M +ATEYLLT +R +S E++ +MRAIAMSL D+ Q + + E +E+ ++++ +++EE E L + + FT LPGC +L+D LP+TV+R CDL++ RNG ++++ IL+ ++ +V A DVL+ L L S + + E + + +++ A RI L LLFEE KL CA +VE S ++ ++ LL V L+ V TPKW+TP+LL ID YEK + RRA +T + W+W+D SGRWC Y+ ++N TID A+ SGETSVR T GRR+YT+QF TM+Q NEETGNRRP+M+++ +K S+++ + + + M++ +E K SD EE I I +GL D + L++ACV + +PVD D L+A LRLCLRLT++ + A+ FA L +++L L QSS F+G T L TLL+RH++EDP TLRHTMEKV+R TS S T+ S+EINYILRVL PAACR+P IF VA +R P+P +D E+ R+ LVK+ P K S P + + + VI D+LN L P+ + S+ + + +L + G R++ + D G V +TSA S+D KK P + S + RLLAELV+SY G A LI ++ Y G E IKED S LAF+LD LL + DKD L + +A+LA+ +AQ ALV EVK AL R+L ES KH+++QA+ C+IST++ESCPS+ A + MNNI+++ LK+GL+ DLARVPH+LDLSSPN+A T+N+ALKPLETLSRIVNQP++L S S + +A+DS SN +P G + ++ E + +V T +E A GD D EA E D A + S ++Q + + ++++D+LLERD GS N TII
Sbjct: 1327 DMGFTREHAMEALLNTSTMEQATEYLLTHPPPI------------MGGVVRDLSMS-EEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNNNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKTSDTPLALDSTNTEKETSLEETKIGEI-LIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKP--SPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGGMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDAQTGFSINSQVFAADGSSTETSASGVSQGEVSTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAASGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPST-SSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKSKSEQDAQGAAQDSNSNQQDPGEPGET----EVQEEDHDV------TQTEV--ADGDIM---DGEA-ETDSVVIAGQPEVLSSQEMQV-----ENELEDLIDELLERDGGSGNSTII 2368 HSP 3 Score: 363.999 bits (933), Expect = 4.674e-99 Identity = 171/283 (60.42%), Postives = 206/283 (72.79%), Query Frame = 0
Query: 12 TTIEKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNL---SIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSK---GSKGEDDKNSGGGA 288
T + K +K P + +IKQIKPLL ASSRLGRALAELF LLVKLCVGSP+RQRR H T AP+ A + ASAL +LL GLSW PPP +P P FRLTFF CSVGFT PMLFD++KYPYHLML KF+ SGG A F+TF WALS GGK+ EGLE ++LPDGTGEFLD+WLML+E+MVNP VLESPH+LP + NF F +++L+ T K AF + +W + PLKVYG RMAES+L+ILCHIL+GE +I E+LSK GS+GE+D G
Sbjct: 1028 TASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVAEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGG 1310 HSP 4 Score: 295.434 bits (755), Expect = 2.889e-78 Identity = 261/766 (34.07%), Postives = 369/766 (48.17%), Query Frame = 0
Query: 1467 GNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFR-----RSGAPLMM-----DVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE---------NTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAAT-----TALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVP-------PLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRN--MDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDV--SVTGSSSSTTGGDVSMESDQP---------------RESEEAI-GGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS------NVPEVNPEFLAALPPNIQEEVLAQQRMEQQR-QLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNS-LSSILRGSV--SRL----GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
G R+ + R L G+++ ++ N + P+ P ILQ LG S +++ R A L++ ++ S + +D+ D +AT +G LSSIP+AL RW EE +V+D ESMHDCV+ +K I+ +E ++ EEE KI K D N D+ A + T TE G TP+ P TPS+T AT T +T S+ A+ ++ Q+L + I +++P P S TT + S + S + + ++SQ MDT++ A T + + + +P S+ T SST V V G GD S E QP R+S AI G S P+PSTS ++P L + +PEGVDPSFLAALP+++R+EV+ +Q ++ + A N V EV+PEFLAALPP IQEEVLAQQR EQQR +L+Q + + P+DP F QTLP+ LR+SVL DMEDS +A +P ++AAEAQ+LRRE E R R MHER F + S LS+ILR SRL G QY + F++ GS + R + + +R +GR LLD+E LSCLL+LLFVD+ K+NT+RLHR+LRNLCYHA TR WV+R L+SIL+R++E++
Sbjct: 2542 GIGRSQRTLRQLTANTGHTIHVHYPGNRQ----PNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAG---TLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQS-AQCAASKTNDSTEQNLSDG-------TPMPDSYPA---------------------TPSSTDVATSEPRETLVTLQPSQQQPTLPTPPALGEIPQELQSPIGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCT--------------LEEAVGDAPATGSSEQPTAGSSTPGDAPPVVTEVQGR------GDGSGEPTQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDP-----LAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNNSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSS---------SHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE 3237 HSP 5 Score: 68.1662 bits (165), Expect = 1.628e-9 Identity = 48/123 (39.02%), Postives = 65/123 (52.85%), Query Frame = 0
Query: 2257 ISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDL---------------QNELLSIKSTNFEHDRSGKDKGGLLDRF-TKETVVLNAPSKQ 2363
++ + L+L+V+VLTS SCSEEGLED +LL LS G TRD +L+LLL GA+ LG + + L Q E LS E ++ K KG + RF E VV+ A K+
Sbjct: 3577 LTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDPGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGMPEEQPQTTKLKGKMQSRFDVAENVVIVASQKR 3699
BLAST of EMLSAG00000004991 vs. GO
Match: - (symbol:HUWE1 "Uncharacterized protein" species:9823 "Sus scrofa" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] Pfam:PF00632 InterPro:IPR000449 InterPro:IPR000569 InterPro:IPR009060 InterPro:IPR016024 Pfam:PF00627 PROSITE:PS50237 SMART:SM00119 SUPFAM:SSF48371 GO:GO:0016567 InterPro:IPR015940 SMART:SM00165 SUPFAM:SSF46934 GO:GO:0004842 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 PROSITE:PS50030 InterPro:IPR004170 Pfam:PF02825 PROSITE:PS50918 InterPro:IPR025527 InterPro:IPR010309 InterPro:IPR010314 Pfam:PF14377 Pfam:PF06012 Pfam:PF06025 EMBL:CU302239 EMBL:CU695116 Ensembl:ENSSSCT00000013481 ArrayExpress:F1RUH0 Uniprot:F1RUH0) HSP 1 Score: 838.18 bits (2164), Expect = 0.000e+0 Identity = 400/568 (70.42%), Postives = 467/568 (82.22%), Query Frame = 0
Query: 2412 EENKMDVDENS----------SDSTSEAKANSQWK---IESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQL----------ADNKEME---SSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
EE+ MDVD+ S SD T + + + + + LSEQLSLD LW+ LG+CLK+L E+ D HAVLVLQ +VEAFFLVHA T+ + K + ++TR+ QLAHI+++ A ++ S +S S+SL PDT+KFL FAETHRTVLNQILRQST HLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE LR+ED+AVHVRR++VFE+S+REL R+SPEE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 3807 EESPMDVDQPSPSAQDTQSIGSDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHA--TERESKPPI--RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 4370 HSP 2 Score: 382.104 bits (980), Expect = 1.516e-104 Identity = 251/643 (39.04%), Postives = 363/643 (56.45%), Query Frame = 0
Query: 397 DMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTE--GNNEEDSQQQSGKEEEENEGGSFG-------LSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSP-IEEDVFRTT---ESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCS--GSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVC---SRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK-----SKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPN--------------EEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIR---TSTLNSQTT--------SKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETD---EDTRL-------LVKSLPTKAPNSVAPPLGEVSQSVICDLLNFL 980
DMGF+RE +EA+ T M +ATEYLLT +R +S E++ +MRAIAMSL D+ Q + + E +E+ ++++ +++EE E L + + FT LPGC +L+D LP+TV+R CDL++ RNG ++++ IL+ ++ +V A DVL+ L L S + + E + + +++ A RI L LLFEE KL CA +VE S ++ ++ LL V L+ V TPKW+TP+LL ID YEK + RRA +T + W+W+D SGRWC Y+ ++N TID A+ SGETSVR T GRR+YT+QF TM+Q NEETGNRRP+M+++ +K S+++ + + + M++ +E K +D EE I I +GL D + L++ACV + +PVD D L+A LRLCLRLT++ + A+ FA L +++L L QSS F+G T L TLL+RH++EDP TLRHTMEKV+R TS S T+ S+EINYILRVL PAACR+P IF VA +R P+P +D E+ R+ LVK+ P K S P + + + VI D+LN L
Sbjct: 1326 DMGFTREHAMEALLNTSTMEQATEYLLTHPPPI------------MGGVVRDLSMS-EEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNNNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKANDTPLALDSTNTEKETSLEETKIGEI-LIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKP--SPLPVIPDTIKEVIYDMLNAL 1952 HSP 3 Score: 363.229 bits (931), Expect = 8.209e-99 Identity = 170/280 (60.71%), Postives = 205/280 (73.21%), Query Frame = 0
Query: 15 EKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNL---SIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSK---GSKGEDDKNSGGGA 288
+ K +K P + +IKQIKPLL ASSRLGRALAELF LLVKLCVGSP+RQRR H T AP+ A + ASAL +LL GLSW PPP +P P FRLTFF CSVGFT PMLFD++KYPYHLML KF+ SGG A F+TF WALS GGK+ EGLE ++LPDGTGEFLD+WLML+E+MVNP VLESPH+LP + NF F +++L+ T K AF + +W + PLKVYG RMAES+L+ILCHIL+GE +I E+LSK GS+GE+D G
Sbjct: 1030 DSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVAEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGG 1309 HSP 4 Score: 293.508 bits (750), Expect = 1.288e-77 Identity = 261/769 (33.94%), Postives = 369/769 (47.98%), Query Frame = 0
Query: 1467 GNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFR-----RSGAPLMM-----DVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE---------NTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAAT-----TALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVP-------PLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRN--MDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTT-----GGDVSMESDQP---------------RESEEAI-GGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS------NVPEVNPEFLAALPPNIQEEVLAQQRMEQQR-QLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNS-LSSILRGSV--SRL----GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
G R+ + R L G+++ ++ N + P+ P ILQ LG S +++ R A L++ ++ S + +D+ D +AT +G LSSIP+AL RW EE +V+D ESMHDCV+ +K I+ +E ++ EEE KI K D N D+ A T + T TE TP+ P TTPS+T AAT T +T S+ A+ ++ Q+L + +++P P S TT + S + S + + ++SQ MDT++ A T E + A +S TAG+ S G + GD S E QP R+S AI G S P+PSTS ++P L + +PEGVDPSFLAALP+++R+EV+ +Q ++ + A N+ V EV+PEFLAALPP IQEEVLAQQR EQQR +L+Q + + P+DP F QTLP+ LR+SVL DMEDS +A +P ++AAEAQ+LRRE E R R MHER F + S LS+ILR SRL G QY + F++ GS + R + + +R +GR LLD+E LSCLL+LLFVD+ K+NT+RLHR+LRNLCYHA TR WV+R L+SIL+R++E++
Sbjct: 2541 GIGRSQRTLRQLTANTGHTIHVHYPGNRQ----PNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAG---TLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQS-AQCTASKTNDSTEQNLSEKD-----GTPMPDSYP---------------------TTPSSTDAATSEPKETLVTLQPSQQQPTLPPPPALGEIPQELQSPTGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCT---------------------LEEAVGDTSAAGSSEQPTAGS--STPGDAPPVVPDVQGRGDGSGEPTQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDP-----LAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSS---------SHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE 3238 HSP 5 Score: 67.781 bits (164), Expect = 2.560e-9 Identity = 48/123 (39.02%), Postives = 65/123 (52.85%), Query Frame = 0
Query: 2257 ISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDL---------------QNELLSIKSTNFEHDRSGKDKGGLLDRF-TKETVVLNAPSKQ 2363
++ + L+L+V+VLTS SCSEEGLED +LL LS G TRD +L+LLL GA+ LG + + L Q E LS E ++ K KG + RF E VV+ A K+
Sbjct: 3577 LTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDPGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKR 3699
BLAST of EMLSAG00000004991 vs. GO
Match: - (symbol:HUWE1 "Uncharacterized protein" species:9823 "Sus scrofa" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] Pfam:PF00632 InterPro:IPR000449 InterPro:IPR000569 InterPro:IPR009060 InterPro:IPR016024 Pfam:PF00627 PROSITE:PS50237 SMART:SM00119 SUPFAM:SSF48371 GO:GO:0016567 InterPro:IPR015940 SMART:SM00165 SUPFAM:SSF46934 GO:GO:0004842 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 PROSITE:PS50030 InterPro:IPR004170 Pfam:PF02825 PROSITE:PS50918 InterPro:IPR025527 InterPro:IPR010309 InterPro:IPR010314 Pfam:PF14377 Pfam:PF06012 Pfam:PF06025 OrthoDB:EOG7G7KN8 EMBL:CU302239 EMBL:CU695116 Ensembl:ENSSSCT00000035055 ArrayExpress:K7GNJ7 Uniprot:K7GNJ7) HSP 1 Score: 838.18 bits (2164), Expect = 0.000e+0 Identity = 400/568 (70.42%), Postives = 467/568 (82.22%), Query Frame = 0
Query: 2412 EENKMDVDENS----------SDSTSEAKANSQWK---IESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQL----------ADNKEME---SSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
EE+ MDVD+ S SD T + + + + + LSEQLSLD LW+ LG+CLK+L E+ D HAVLVLQ +VEAFFLVHA T+ + K + ++TR+ QLAHI+++ A ++ S +S S+SL PDT+KFL FAETHRTVLNQILRQST HLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE LR+ED+AVHVRR++VFE+S+REL R+SPEE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 3805 EESPMDVDQPSPSAQDTQSIGSDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHA--TERESKPPI--RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 4368 HSP 2 Score: 382.104 bits (980), Expect = 1.478e-104 Identity = 251/643 (39.04%), Postives = 363/643 (56.45%), Query Frame = 0
Query: 397 DMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTE--GNNEEDSQQQSGKEEEENEGGSFG-------LSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSP-IEEDVFRTT---ESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCS--GSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVC---SRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK-----SKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPN--------------EEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIR---TSTLNSQTT--------SKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETD---EDTRL-------LVKSLPTKAPNSVAPPLGEVSQSVICDLLNFL 980
DMGF+RE +EA+ T M +ATEYLLT +R +S E++ +MRAIAMSL D+ Q + + E +E+ ++++ +++EE E L + + FT LPGC +L+D LP+TV+R CDL++ RNG ++++ IL+ ++ +V A DVL+ L L S + + E + + +++ A RI L LLFEE KL CA +VE S ++ ++ LL V L+ V TPKW+TP+LL ID YEK + RRA +T + W+W+D SGRWC Y+ ++N TID A+ SGETSVR T GRR+YT+QF TM+Q NEETGNRRP+M+++ +K S+++ + + + M++ +E K +D EE I I +GL D + L++ACV + +PVD D L+A LRLCLRLT++ + A+ FA L +++L L QSS F+G T L TLL+RH++EDP TLRHTMEKV+R TS S T+ S+EINYILRVL PAACR+P IF VA +R P+P +D E+ R+ LVK+ P K S P + + + VI D+LN L
Sbjct: 1326 DMGFTREHAMEALLNTSTMEQATEYLLTHPPPI------------MGGVVRDLSMS-EEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNNNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKANDTPLALDSTNTEKETSLEETKIGEI-LIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKP--SPLPVIPDTIKEVIYDMLNAL 1952 HSP 3 Score: 363.229 bits (931), Expect = 8.069e-99 Identity = 170/280 (60.71%), Postives = 205/280 (73.21%), Query Frame = 0
Query: 15 EKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNL---SIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSK---GSKGEDDKNSGGGA 288
+ K +K P + +IKQIKPLL ASSRLGRALAELF LLVKLCVGSP+RQRR H T AP+ A + ASAL +LL GLSW PPP +P P FRLTFF CSVGFT PMLFD++KYPYHLML KF+ SGG A F+TF WALS GGK+ EGLE ++LPDGTGEFLD+WLML+E+MVNP VLESPH+LP + NF F +++L+ T K AF + +W + PLKVYG RMAES+L+ILCHIL+GE +I E+LSK GS+GE+D G
Sbjct: 1030 DSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVAEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGG 1309 HSP 4 Score: 296.204 bits (757), Expect = 2.047e-78 Identity = 262/769 (34.07%), Postives = 370/769 (48.11%), Query Frame = 0
Query: 1467 GNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFR-----RSGAPLMM-----DVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE---------NTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAAT-----TALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVP-------PLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRN--MDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTT-----GGDVSMESDQP---------------RESEEAI-GGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS------NVPEVNPEFLAALPPNIQEEVLAQQRMEQQR-QLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNS-LSSILRGSV--SRL----GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
G R+ + R L G+++ ++ N + P+ P ILQ LG S +++ R A L++ ++ S + +D+ D +AT +G LSSIP+AL RW EE +V+D ESMHDCV+ +K I+ +E ++ EEE KI K D N D+ A T + T TE G TP+ P TTPS+T AAT T +T S+ A+ ++ Q+L + +++P P S TT + S + S + + ++SQ MDT++ A T E + A +S TAG+ S G + GD S E QP R+S AI G S P+PSTS ++P L + +PEGVDPSFLAALP+++R+EV+ +Q ++ + A N+ V EV+PEFLAALPP IQEEVLAQQR EQQR +L+Q + + P+DP F QTLP+ LR+SVL DMEDS +A +P ++AAEAQ+LRRE E R R MHER F + S LS+ILR SRL G QY + F++ GS + R + + +R +GR LLD+E LSCLL+LLFVD+ K+NT+RLHR+LRNLCYHA TR WV+R L+SIL+R++E++
Sbjct: 2541 GIGRSQRTLRQLTANTGHTIHVHYPGNRQ----PNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAG---TLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQS-AQCTASKTNDSTEQNLSDG-------TPMPDSYP---------------------TTPSSTDAATSEPKETLVTLQPSQQQPTLPPPPALGEIPQELQSPTGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCT---------------------LEEAVGDTSAAGSSEQPTAGS--STPGDAPPVVPDVQGRGDGSGEPTQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDP-----LAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSS---------SHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE 3236 HSP 5 Score: 67.781 bits (164), Expect = 2.538e-9 Identity = 48/123 (39.02%), Postives = 65/123 (52.85%), Query Frame = 0
Query: 2257 ISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDL---------------QNELLSIKSTNFEHDRSGKDKGGLLDRF-TKETVVLNAPSKQ 2363
++ + L+L+V+VLTS SCSEEGLED +LL LS G TRD +L+LLL GA+ LG + + L Q E LS E ++ K KG + RF E VV+ A K+
Sbjct: 3575 LTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDPGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKR 3697
BLAST of EMLSAG00000004991 vs. GO
Match: - (symbol:HUWE1 "Uncharacterized protein" species:9823 "Sus scrofa" [GO:0000209 "protein polyubiquitination" evidence=IEA] [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0005737 "cytoplasm" evidence=IEA] [GO:0006284 "base-excision repair" evidence=IEA] [GO:0006513 "protein monoubiquitination" evidence=IEA] [GO:0016574 "histone ubiquitination" evidence=IEA] Pfam:PF00632 InterPro:IPR000449 InterPro:IPR000569 InterPro:IPR009060 InterPro:IPR016024 Pfam:PF00627 PROSITE:PS50237 SMART:SM00119 GO:GO:0005737 SUPFAM:SSF48371 GO:GO:0006284 InterPro:IPR015940 SMART:SM00165 SUPFAM:SSF46934 GO:GO:0004842 GO:GO:0000209 TreeFam:TF323417 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 GO:GO:0016574 GO:GO:0006513 PROSITE:PS50030 InterPro:IPR004170 Pfam:PF02825 PROSITE:PS50918 OMA:GMTQEVG InterPro:IPR025527 InterPro:IPR010309 InterPro:IPR010314 Pfam:PF14377 Pfam:PF06012 Pfam:PF06025 EMBL:CU302239 EMBL:CU695116 Ensembl:ENSSSCT00000032330 ArrayExpress:I3L770 Uniprot:I3L770) HSP 1 Score: 837.795 bits (2163), Expect = 0.000e+0 Identity = 400/569 (70.30%), Postives = 467/569 (82.07%), Query Frame = 0
Query: 2412 EENKMDVDENS-----------SDSTSEAKANSQWK---IESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQL----------ADNKEME---SSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
EE+ MDVD+ S SD T + + + + + LSEQLSLD LW+ LG+CLK+L E+ D HAVLVLQ +VEAFFLVHA T+ + K + ++TR+ QLAHI+++ A ++ S +S S+SL PDT+KFL FAETHRTVLNQILRQST HLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE LR+ED+AVHVRR++VFE+S+REL R+SPEE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 3807 EESPMDVDQPSPSAQDTQSIEGSDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHA--TERESKPPI--RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 4371 HSP 2 Score: 382.104 bits (980), Expect = 1.466e-104 Identity = 251/643 (39.04%), Postives = 363/643 (56.45%), Query Frame = 0
Query: 397 DMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTE--GNNEEDSQQQSGKEEEENEGGSFG-------LSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSP-IEEDVFRTT---ESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCS--GSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVC---SRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK-----SKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPN--------------EEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIR---TSTLNSQTT--------SKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETD---EDTRL-------LVKSLPTKAPNSVAPPLGEVSQSVICDLLNFL 980
DMGF+RE +EA+ T M +ATEYLLT +R +S E++ +MRAIAMSL D+ Q + + E +E+ ++++ +++EE E L + + FT LPGC +L+D LP+TV+R CDL++ RNG ++++ IL+ ++ +V A DVL+ L L S + + E + + +++ A RI L LLFEE KL CA +VE S ++ ++ LL V L+ V TPKW+TP+LL ID YEK + RRA +T + W+W+D SGRWC Y+ ++N TID A+ SGETSVR T GRR+YT+QF TM+Q NEETGNRRP+M+++ +K S+++ + + + M++ +E K +D EE I I +GL D + L++ACV + +PVD D L+A LRLCLRLT++ + A+ FA L +++L L QSS F+G T L TLL+RH++EDP TLRHTMEKV+R TS S T+ S+EINYILRVL PAACR+P IF VA +R P+P +D E+ R+ LVK+ P K S P + + + VI D+LN L
Sbjct: 1326 DMGFTREHAMEALLNTSTMEQATEYLLTHPPPI------------MGGVVRDLSMS-EEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNNNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKANDTPLALDSTNTEKETSLEETKIGEI-LIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKP--SPLPVIPDTIKEVIYDMLNAL 1952 HSP 3 Score: 363.229 bits (931), Expect = 8.141e-99 Identity = 170/280 (60.71%), Postives = 205/280 (73.21%), Query Frame = 0
Query: 15 EKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNL---SIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSK---GSKGEDDKNSGGGA 288
+ K +K P + +IKQIKPLL ASSRLGRALAELF LLVKLCVGSP+RQRR H T AP+ A + ASAL +LL GLSW PPP +P P FRLTFF CSVGFT PMLFD++KYPYHLML KF+ SGG A F+TF WALS GGK+ EGLE ++LPDGTGEFLD+WLML+E+MVNP VLESPH+LP + NF F +++L+ T K AF + +W + PLKVYG RMAES+L+ILCHIL+GE +I E+LSK GS+GE+D G
Sbjct: 1030 DSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVAEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGG 1309 HSP 4 Score: 293.508 bits (750), Expect = 1.277e-77 Identity = 261/769 (33.94%), Postives = 369/769 (47.98%), Query Frame = 0
Query: 1467 GNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFR-----RSGAPLMM-----DVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE---------NTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAAT-----TALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVP-------PLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRN--MDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTT-----GGDVSMESDQP---------------RESEEAI-GGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS------NVPEVNPEFLAALPPNIQEEVLAQQRMEQQR-QLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNS-LSSILRGSV--SRL----GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
G R+ + R L G+++ ++ N + P+ P ILQ LG S +++ R A L++ ++ S + +D+ D +AT +G LSSIP+AL RW EE +V+D ESMHDCV+ +K I+ +E ++ EEE KI K D N D+ A T + T TE TP+ P TTPS+T AAT T +T S+ A+ ++ Q+L + +++P P S TT + S + S + + ++SQ MDT++ A T E + A +S TAG+ S G + GD S E QP R+S AI G S P+PSTS ++P L + +PEGVDPSFLAALP+++R+EV+ +Q ++ + A N+ V EV+PEFLAALPP IQEEVLAQQR EQQR +L+Q + + P+DP F QTLP+ LR+SVL DMEDS +A +P ++AAEAQ+LRRE E R R MHER F + S LS+ILR SRL G QY + F++ GS + R + + +R +GR LLD+E LSCLL+LLFVD+ K+NT+RLHR+LRNLCYHA TR WV+R L+SIL+R++E++
Sbjct: 2541 GIGRSQRTLRQLTANTGHTIHVHYPGNRQ----PNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAG---TLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQS-AQCTASKTNDSTEQNLSEKD-----GTPMPDSYP---------------------TTPSSTDAATSEPKETLVTLQPSQQQPTLPPPPALGEIPQELQSPTGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCT---------------------LEEAVGDTSAAGSSEQPTAGS--STPGDAPPVVPDVQGRGDGSGEPTQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDP-----LAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSS---------SHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE 3238 HSP 5 Score: 67.781 bits (164), Expect = 2.539e-9 Identity = 48/123 (39.02%), Postives = 65/123 (52.85%), Query Frame = 0
Query: 2257 ISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDL---------------QNELLSIKSTNFEHDRSGKDKGGLLDRF-TKETVVLNAPSKQ 2363
++ + L+L+V+VLTS SCSEEGLED +LL LS G TRD +L+LLL GA+ LG + + L Q E LS E ++ K KG + RF E VV+ A K+
Sbjct: 3577 LTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDPGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKR 3699
BLAST of EMLSAG00000004991 vs. GO
Match: - (symbol:Huwe1 "HECT, UBA and WWE domain containing 1" species:10090 "Mus musculus" [GO:0000209 "protein polyubiquitination" evidence=ISO] [GO:0003677 "DNA binding" evidence=IEA] [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISO;IBA] [GO:0005634 "nucleus" evidence=ISO;IBA] [GO:0005737 "cytoplasm" evidence=ISO;IBA] [GO:0006281 "DNA repair" evidence=IEA] [GO:0006284 "base-excision repair" evidence=ISO] [GO:0006513 "protein monoubiquitination" evidence=ISO] [GO:0006974 "cellular response to DNA damage stimulus" evidence=IEA] [GO:0016574 "histone ubiquitination" evidence=ISO] [GO:0016874 "ligase activity" evidence=IEA] [GO:0030154 "cell differentiation" evidence=IEA] [GO:0042787 "protein ubiquitination involved in ubiquitin-dependent protein catabolic process" evidence=IBA] [GO:0044822 "poly(A) RNA binding" evidence=ISO] [GO:0070062 "extracellular vesicular exosome" evidence=ISO] Pfam:PF00632 InterPro:IPR000449 InterPro:IPR000569 InterPro:IPR009060 InterPro:IPR016024 Pfam:PF00627 PROSITE:PS50237 SMART:SM00119 UniPathway:UPA00143 MGI:MGI:1926884 GO:GO:0005634 GO:GO:0005737 GO:GO:0030154 SUPFAM:SSF48371 GO:GO:0006284 GO:GO:0003677 InterPro:IPR015940 SMART:SM00165 SUPFAM:SSF46934 GO:GO:0004842 GO:GO:0000209 eggNOG:COG5021 GO:GO:0042787 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 GO:GO:0016574 GO:GO:0006513 PROSITE:PS50030 InterPro:IPR004170 Pfam:PF02825 PROSITE:PS50918 CTD:10075 HOVERGEN:HBG080254 KO:K10592 ChiTaRS:HUWE1 InterPro:IPR025527 InterPro:IPR010309 InterPro:IPR010314 Pfam:PF14377 Pfam:PF06012 Pfam:PF06025 EMBL:AY772010 EMBL:AY929611 EMBL:BX571795 EMBL:AL672180 EMBL:AL954855 EMBL:AB093227 EMBL:BC011391 EMBL:BC017642 EMBL:BC054372 EMBL:BC070444 EMBL:AK083499 EMBL:AB025966 RefSeq:NP_067498.4 UniGene:Mm.27372 UniGene:Mm.488413 ProteinModelPortal:Q7TMY8 SMR:Q7TMY8 BioGrid:208493 IntAct:Q7TMY8 MINT:MINT-4139570 STRING:10090.ENSMUSP00000026292 PhosphoSite:Q7TMY8 PaxDb:Q7TMY8 PRIDE:Q7TMY8 Ensembl:ENSMUST00000112622 GeneID:59026 KEGG:mmu:59026 UCSC:uc009upq.2 UCSC:uc009upr.1 NextBio:314602 PRO:PR:Q7TMY8 ArrayExpress:Q7TMY8 Bgee:Q7TMY8 CleanEx:MM_HUWE1 Genevestigator:Q7TMY8 Uniprot:Q7TMY8) HSP 1 Score: 836.254 bits (2159), Expect = 0.000e+0 Identity = 402/569 (70.65%), Postives = 466/569 (81.90%), Query Frame = 0
Query: 2412 EENKMDVDENS-----------SDSTSEAKANSQWKIESL---SEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQL----------ADNKEME---SSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
EE+ MDVD+ S SD T + + + K L SEQLSLD LW+ LG+CLK+L E+ D HAVLVLQ +VEAFFLVHA T+ + K V ++TR+ QLAHI+++ A ++ S +S S+SL PDT+KFL FAETHRTVLNQILRQST HLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE LR+ED+AVHVRR++VFE+S+REL R+SPEE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 3808 EESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHA--TERESKPPV--RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 4372 HSP 2 Score: 386.726 bits (992), Expect = 7.161e-106 Identity = 254/646 (39.32%), Postives = 364/646 (56.35%), Query Frame = 0
Query: 397 DMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTE--GNNEEDSQQQSGKEEEENEGGSFG-------LSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSP-IEEDVFRTT---ESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCS--GSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVC---SRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIKSKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPNEEDVITTIPTA----------------------EGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIR---TSTLNSQTT--------SKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETD---EDTRL-------LVKSLPTKAPNSVAPPLGEVSQSVICDLLNFL 980
DMGF+RE +EA+ T M +ATEYLLT +R +S E++ +MRAIAMSL D+ Q + + E +E+ ++++ +++EE E L + + FT LPGC +L+D LP+TV+R CDL++ RNG ++++ IL+ ++ +V A DVL+ L L S + + E + + +++ A RI L LLFEE KL CA +VE S ++ ++ LL V L+ V TPKW+TP+LL ID YEK + RRA +T S W+W+D SGRWC Y+ ++N TID A+ SGETSVR T GRR+YT+QF TM+Q NEETGNRRP+M+++ R + ++ SSN + EE K+ ++E+ IP A +GL D + L++ACV + +PVD D L+A LRLCLRLT++ + A+ FA L +++L L QSS F+G T L TLL+RH++EDP TLRHTMEKV+R TS S T+ S+EINYILRVL PAACR+P IF VA +R P+P +D E+ R+ LVK+ P K P+S+ P + + + VI D+LN L
Sbjct: 1327 DMGFTREHAMEALLNTSTMEQATEYLLTHPPPI------------IGGVVRDLSMS-EEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLL----RVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLK-PSSL-PVIPDTIKEVIYDMLNAL 1953 HSP 3 Score: 353.214 bits (905), Expect = 9.653e-96 Identity = 162/258 (62.79%), Postives = 195/258 (75.58%), Query Frame = 0
Query: 24 PCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNL---SIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKG 278
P + +IKQIKPLL ASSRLGRALAELF LLVKLCVGSP+RQRR H T AP+ A + ASAL +LL GLSW PPP +P P FRLTFF CSVGFT PMLFD++KYPYHLML KF+ SGG A F+TF WALS GGK+ EGLE ++LPDGTGEFLD+WLML+E+MVNP VLESPH+LP + +F F +++L+ T K AF + +W + PLKVYG RMAES+L+ILCHIL+GE +I E+LSK +G
Sbjct: 1040 PAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEG 1297 HSP 4 Score: 298.516 bits (763), Expect = 3.395e-79 Identity = 260/759 (34.26%), Postives = 371/759 (48.88%), Query Frame = 0
Query: 1467 GNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFR-----RSGAPLMM-----DVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE---------NTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAATTALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVP-------PLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRN--MDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQP---------------RESEEAI-GGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQ------RRLQNIRQRAQQQNTEGSNVPEVNPEFLAALPPNIQEEVLAQQRMEQQR-QLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNS-LSSILRGSV--SRL----GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
G R+ + R L G+++ ++ N P+ P ILQ LG S +++ R A L++ ++ S + +D+ D +AT +G LSSIP+AL RW EE +V+D ESMHDCV+ +K I+ +E ++ EEE KII K D N D+ A T T T TE G TP+ P TT ++T T+ + T T S+ A ++ ++ Q+L + +++P P S TT + S + S + + ++SQ MDT++ A T + + + +P A+ S T+ ++T G SV DVS ES+QP R+S AI G S P+PSTS ++P L + +PEGVDPSFLAALP+++R+EV+ +Q R + V EV+PEFLAALPP IQEEVLAQQR EQQR +L+Q + + P+DP F QTLP+ LR+SVL DMEDS +A +P ++AAEAQ+LRRE E R R MHER F + S LS+ILR SRL G QY + F++ GS + R + + +R +GR LLD+E LSCLL+LLFVD+ K+NT+RLHR+LRNLCYHA TR WV+R L+SIL+R++E++
Sbjct: 2542 GIGRSQRTLRQLTANTGHTIHVHYPGN----RQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAG---TLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQS-AQCTVTKTNDSTEQNVSDG-------TPMPDSYP----------------TTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCT--------------LEEAVGDTP--AAGSSEQPTAGSSTPGDAPSVVAEVQGRP--DVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDP-----LAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSS---------SHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE 3237 HSP 5 Score: 68.5514 bits (166), Expect = 1.389e-9 Identity = 53/147 (36.05%), Postives = 74/147 (50.34%), Query Frame = 0
Query: 2239 KGTGSKEESGSGNN------LPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDL---------------QNELLSIKSTNFEHDRSGKDKGGLLDRF-TKETVVLNAPSKQ 2363
KG+ S + G G + + ++ + L+L+V+VLTS SCSEEGLED +LL LS G TRD +L+LLL GA+ LG + + L Q E LS E ++ K KG + RF E VV+ A K+
Sbjct: 3553 KGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKR 3699
BLAST of EMLSAG00000004991 vs. C. finmarchicus
Match: gi|592944255|gb|GAXK01014298.1| (TSA: Calanus finmarchicus comp8631_c3_seq10 transcribed RNA sequence) HSP 1 Score: 830.091 bits (2143), Expect = 0.000e+0 Identity = 413/505 (81.78%), Postives = 448/505 (88.71%), Query Frame = 0
Query: 2453 LGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMEXXXXXXXXXXXXXXPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTXXXXXXXXXXXXXXAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
L +CLK+LA+TPDHHAVLVLQ +VEAFFLVHAA +EKKK QKETR+EQLAHI+E+ E E + S +SPDTKKFLAFAETHRTVLNQILRQST HLADGPFSVLVDHTRVLDFDIKRRYFRTELER+DE +RREDLAVHVRR+ VFEESFREL RRS EE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNP YALFK+SPGD+VTY I + S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHIL K VK+ DMESEDY FYKGL+FL+ +KVSDLGYDLTFSTEIQEFGVTE+RDLIP+GRN+ VT++ K DYIRLVCQMKMTGAIRKQL+AFLEGFY+IIPRRLI+IFNEQELELLLSGLP I+IDDLK+N+EYHKY SLQI+WFWRALRSFDQTD+AKF+QFVTGSSKVPLQGF LEGMNG QKFQIHRDDRSTDRLP+AHTCFNQLDLPAYETYDKLRTYLLKAI ECSE F
Sbjct: 158 LSNCLKELADTPDHHAVLVLQPTVEAFFLVHAAVISSEEKKKPNQKETRKEQLAHIEEK-EGQLESEQAPQTVVGSEEDISPDTKKFLAFAETHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERLDEGIRREDLAVHVRRDAVFEESFRELHRRSAEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPNYALFKSSPGDRVTYTINDFSHINSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKLVKHQDMESEDYEFYKGLDFLLENKVSDLGYDLTFSTEIQEFGVTEVRDLIPDGRNVIVTDQNKADYIRLVCQMKMTGAIRKQLAAFLEGFYDIIPRRLIAIFNEQELELLLSGLPDINIDDLKANSEYHKYSVTSLQIVWFWRALRSFDQTDRAKFLQFVTGSSKVPLQGFGALEGMNGAQKFQIHRDDRSTDRLPAAHTCFNQLDLPAYETYDKLRTYLLKAIQECSEGFGF 1669
BLAST of EMLSAG00000004991 vs. C. finmarchicus
Match: gi|592944256|gb|GAXK01014297.1| (TSA: Calanus finmarchicus comp8631_c3_seq9 transcribed RNA sequence) HSP 1 Score: 840.106 bits (2169), Expect = 0.000e+0 Identity = 413/505 (81.78%), Postives = 448/505 (88.71%), Query Frame = 0
Query: 2453 LGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMEXXXXXXXXXXXXXXPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTXXXXXXXXXXXXXXAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
L +CLK+LA+TPDHHAVLVLQ +VEAFFLVHAA +EKKK QKETR+EQLAHI+E+ E E + S +SPDTKKFLAFAETHRTVLNQILRQST HLADGPFSVLVDHTRVLDFDIKRRYFRTELER+DE +RREDLAVHVRR+ VFEESFREL RRS EE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNP YALFK+SPGD+VTY I + S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHIL K VK+ DMESEDY FYKGL+FL+ +KVSDLGYDLTFSTEIQEFGVTE+RDLIP+GRN+ VT++ K DYIRLVCQMKMTGAIRKQL+AFLEGFY+IIPRRLI+IFNEQELELLLSGLP I+IDDLK+N+EYHKY SLQI+WFWRALRSFDQTD+AKF+QFVTGSSKVPLQGF LEGMNG QKFQIHRDDRSTDRLP+AHTCFNQLDLPAYETYDKLRTYLLKAI ECSE F
Sbjct: 1807 LSNCLKELADTPDHHAVLVLQPTVEAFFLVHAAVISSEEKKKPNQKETRKEQLAHIEEK-EGQLESEQAPQTVVGSEEDISPDTKKFLAFAETHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERLDEGIRREDLAVHVRRDAVFEESFRELHRRSAEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPNYALFKSSPGDRVTYTINDFSHINSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKLVKHQDMESEDYEFYKGLDFLLENKVSDLGYDLTFSTEIQEFGVTEVRDLIPDGRNVIVTDQNKADYIRLVCQMKMTGAIRKQLAAFLEGFYDIIPRRLIAIFNEQELELLLSGLPDINIDDLKANSEYHKYSVTSLQIVWFWRALRSFDQTDRAKFLQFVTGSSKVPLQGFGALEGMNGAQKFQIHRDDRSTDRLPAAHTCFNQLDLPAYETYDKLRTYLLKAIQECSEGFGF 3318 HSP 2 Score: 130.568 bits (327), Expect = 4.798e-29 Identity = 61/93 (65.59%), Postives = 77/93 (82.80%), Query Frame = 0
Query: 2100 NSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVK 2192
N+++F+GRQLLD+EGLSCLLILLF+DD+K+NTTRLHRILRNLCYHAPTRDWVV+CL+SILE+AN + A Q + P+ S ++T TP K
Sbjct: 85 NNVKFRGRQLLDHEGLSCLLILLFIDDAKLNTTRLHRILRNLCYHAPTRDWVVKCLLSILEKANTGAECPA--QQTMADPSTSG-ANTPTPAK 354 HSP 3 Score: 119.783 bits (299), Expect = 8.633e-26 Identity = 91/164 (55.49%), Postives = 113/164 (68.90%), Query Frame = 0
Query: 2205 PVIRRNXXXXXXXXXXXXXXXIGRPDSLNNKDDXXXXXXXXXXXXXNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNF------EHDRSGKDKGGLLDRFTKETVVLNAPSK 2362
PVI+R+SLLTDKLLRLLSLIS+G+PD L D+ E G + + D ++LAV+VLTSK+CSEEGLEDVTALLLNLS+G TR+ IL LLL GA++LGNVV A+V DL EL +K++ E + GK KG + DRFTKE VVL AP+K
Sbjct: 877 PVIKRSSLLTDKLLRLLSLISLGQPDVLKRLDESARQEENTEGGIL--VDKAVKEDQIQLAVEVLTSKACSEEGLEDVTALLLNLSYGGTQTRESILHLLLAGARQLGNVVSAHVSDLLTELAELKASGGLASLPKEEEEEGKHKGVMADRFTKEAVVLVAPTK 1362
BLAST of EMLSAG00000004991 vs. C. finmarchicus
Match: gi|592944293|gb|GAXK01014260.1| (TSA: Calanus finmarchicus comp8631_c2_seq1 transcribed RNA sequence) HSP 1 Score: 843.573 bits (2178), Expect = 0.000e+0 Identity = 413/505 (81.78%), Postives = 448/505 (88.71%), Query Frame = 0
Query: 2453 LGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMEXXXXXXXXXXXXXXPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTXXXXXXXXXXXXXXAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
L +CLK+LA+TPDHHAVLVLQ +VEAFFLVHAA +EKKK QKETR+EQLAHI+E+ E E + S +SPDTKKFLAFAETHRTVLNQILRQST HLADGPFSVLVDHTRVLDFDIKRRYFRTELER+DE +RREDLAVHVRR+ VFEESFREL RRS EE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNP YALFK+SPGD+VTY I + S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHIL K VK+ DMESEDY FYKGL+FL+ +KVSDLGYDLTFSTEIQEFGVTE+RDLIP+GRN+ VT++ K DYIRLVCQMKMTGAIRKQL+AFLEGFY+IIPRRLI+IFNEQELELLLSGLP I+IDDLK+N+EYHKY SLQI+WFWRALRSFDQTD+AKF+QFVTGSSKVPLQGF LEGMNG QKFQIHRDDRSTDRLP+AHTCFNQLDLPAYETYDKLRTYLLKAI ECSE F
Sbjct: 190 LSNCLKELADTPDHHAVLVLQPTVEAFFLVHAAVISSEEKKKPNQKETRKEQLAHIEEK-EGQLESEQAPQTVVGSEEDISPDTKKFLAFAETHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERLDEGIRREDLAVHVRRDAVFEESFRELHRRSAEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPNYALFKSSPGDRVTYTINDFSHINSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKLVKHQDMESEDYEFYKGLDFLLENKVSDLGYDLTFSTEIQEFGVTEVRDLIPDGRNVIVTDQNKADYIRLVCQMKMTGAIRKQLAAFLEGFYDIIPRRLIAIFNEQELELLLSGLPDINIDDLKANSEYHKYSVTSLQIVWFWRALRSFDQTDRAKFLQFVTGSSKVPLQGFGALEGMNGAQKFQIHRDDRSTDRLPAAHTCFNQLDLPAYETYDKLRTYLLKAIQECSEGFGF 1701 HSP 2 Score: 325.865 bits (834), Expect = 1.812e-88 Identity = 197/331 (59.52%), Postives = 245/331 (74.02%), Query Frame = 0
Query: 1877 PIPSTSGTS--QNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS---NVPEVNPEFLAALPPNIQEEVLAXXX-XXXXXXXXXVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMXXXXXXXXXXXQSLRREXEQRNRNFMHERFFNN------GGNSLSSILRGSVSRLGNQYIIGNLVG---REAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVK 2192
P+P +SGTS ++PD+SSILGD++IPEGVDPSFLAALPE+MR EVI++QRRL RQ+ Q G+ + EVNPEFLAALPPNIQEEVLAQQR +Q++ +Q +P APVDPGEF QTLP SLRQS+LADME+SQ++ALPA+LAAEAQ+LRR+ EQRNR MHERFFN+ G +LSSILR +V+RLG+QY I G R S R ++ AN+++F+GRQLLD+EGLSCLLILLF+DD+K+NTTRLHRILRNLCYHAPTRDWVV+CL+SILE+AN + A Q + P+ S ++T TP K
Sbjct: 3154 PLPGSSGTSTIRDPDFSSILGDLDIPEGVDPSFLAALPEDMRAEVIEEQRRLVRARQQPPAQPPPGTTAQGMQEVNPEFLAALPPNIQEEVLAQQRLEQQRQNAAQADPTAPVDPGEFLQTLPPSLRQSLLADMEESQISALPADLAAEAQNLRRDYEQRNRQMMHERFFNHVNHTGPSGPTLSSILRNTVNRLGSQYAIHTGAGGPGRSNLWRSIGR-----GGPGHQQSAAALAAANNVKFRGRQLLDHEGLSCLLILLFIDDAKLNTTRLHRILRNLCYHAPTRDWVVKCLLSILEKANTGAECPA--QQTMADPSTSG-ANTPTPAK 4122 HSP 3 Score: 119.013 bits (297), Expect = 2.218e-25 Identity = 91/164 (55.49%), Postives = 113/164 (68.90%), Query Frame = 0
Query: 2205 PVIRRNXXXXXXXXXXXXXXXIGRPDSLNNKDDXXXXXXXXXXXXXNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNF------EHDRSGKDKGGLLDRFTKETVVLNAPSK 2362
PVI+R+SLLTDKLLRLLSLIS+G+PD L D+ E G + + D ++LAV+VLTSK+CSEEGLEDVTALLLNLS+G TR+ IL LLL GA++LGNVV A+V DL EL +K++ E + GK KG + DRFTKE VVL AP+K
Sbjct: 2146 PVIKRSSLLTDKLLRLLSLISLGQPDVLKRLDESARQEENTEGGIL--VDKAVKEDQIQLAVEVLTSKACSEEGLEDVTALLLNLSYGGTQTRESILHLLLAGARQLGNVVSAHVSDLLTELAELKASGGLASLPKEEEEEGKHKGVMADRFTKEAVVLVAPTK 2631 HSP 4 Score: 104.375 bits (259), Expect = 5.336e-21 Identity = 67/163 (41.10%), Postives = 98/163 (60.12%), Query Frame = 0
Query: 1451 PSHPLLMGRQNLETGTGNARNSASTRSLPRTRGNSLRPYFQLNSR-AQNNPSTPAILQNFLGGSNPQELITF----------GFRRSGAPLMMDV-VGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE 1601
PSHPLLMGR G+AR RSL R RG Y QLN R P TPAILQ+ LG ++ ++ + R + L+M+ G ++LD++++++P L+ G +ALS++P+AL+RW EESRV+DG+S+HDC+T KPE+++VIE
Sbjct: 4948 PSHPLLMGRTEQVVPAGSARGQG--RSLTRQRGFR---YIQLNPRNGGGGPGTPAILQSLLGHNSGRDFLQLTGNHPGLNVGAMRDATRVLVMESGSGFAILDSLEDEIPGLEGLGQGG--GSALSTVPNALVRWTEESRVLDGDSLHDCMTVCKPELLDVIE 5415
BLAST of EMLSAG00000004991 vs. C. finmarchicus
Match: gi|592944257|gb|GAXK01014296.1| (TSA: Calanus finmarchicus comp8631_c3_seq8 transcribed RNA sequence) HSP 1 Score: 843.573 bits (2178), Expect = 0.000e+0 Identity = 413/505 (81.78%), Postives = 448/505 (88.71%), Query Frame = 0
Query: 2453 LGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMEXXXXXXXXXXXXXXPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTXXXXXXXXXXXXXXAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
L +CLK+LA+TPDHHAVLVLQ +VEAFFLVHAA +EKKK QKETR+EQLAHI+E+ E E + S +SPDTKKFLAFAETHRTVLNQILRQST HLADGPFSVLVDHTRVLDFDIKRRYFRTELER+DE +RREDLAVHVRR+ VFEESFREL RRS EE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNP YALFK+SPGD+VTY I + S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHIL K VK+ DMESEDY FYKGL+FL+ +KVSDLGYDLTFSTEIQEFGVTE+RDLIP+GRN+ VT++ K DYIRLVCQMKMTGAIRKQL+AFLEGFY+IIPRRLI+IFNEQELELLLSGLP I+IDDLK+N+EYHKY SLQI+WFWRALRSFDQTD+AKF+QFVTGSSKVPLQGF LEGMNG QKFQIHRDDRSTDRLP+AHTCFNQLDLPAYETYDKLRTYLLKAI ECSE F
Sbjct: 4108 LSNCLKELADTPDHHAVLVLQPTVEAFFLVHAAVISSEEKKKPNQKETRKEQLAHIEEK-EGQLESEQAPQTVVGSEEDISPDTKKFLAFAETHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERLDEGIRREDLAVHVRRDAVFEESFRELHRRSAEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPNYALFKSSPGDRVTYTINDFSHINSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKLVKHQDMESEDYEFYKGLDFLLENKVSDLGYDLTFSTEIQEFGVTEVRDLIPDGRNVIVTDQNKADYIRLVCQMKMTGAIRKQLAAFLEGFYDIIPRRLIAIFNEQELELLLSGLPDINIDDLKANSEYHKYSVTSLQIVWFWRALRSFDQTDRAKFLQFVTGSSKVPLQGFGALEGMNGAQKFQIHRDDRSTDRLPAAHTCFNQLDLPAYETYDKLRTYLLKAIQECSEGFGF 5619 HSP 2 Score: 325.865 bits (834), Expect = 1.899e-88 Identity = 197/331 (59.52%), Postives = 245/331 (74.02%), Query Frame = 0
Query: 1877 PIPSTSGTS--QNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS---NVPEVNPEFLAALPPNIQEEVLAXXX-XXXXXXXXXVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMXXXXXXXXXXXQSLRREXEQRNRNFMHERFFNN------GGNSLSSILRGSVSRLGNQYIIGNLVG---REAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVK 2192
P+P +SGTS ++PD+SSILGD++IPEGVDPSFLAALPE+MR EVI++QRRL RQ+ Q G+ + EVNPEFLAALPPNIQEEVLAQQR +Q++ +Q +P APVDPGEF QTLP SLRQS+LADME+SQ++ALPA+LAAEAQ+LRR+ EQRNR MHERFFN+ G +LSSILR +V+RLG+QY I G R S R ++ AN+++F+GRQLLD+EGLSCLLILLF+DD+K+NTTRLHRILRNLCYHAPTRDWVV+CL+SILE+AN + A Q + P+ S ++T TP K
Sbjct: 1687 PLPGSSGTSTIRDPDFSSILGDLDIPEGVDPSFLAALPEDMRAEVIEEQRRLVRARQQPPAQPPPGTTAQGMQEVNPEFLAALPPNIQEEVLAQQRLEQQRQNAAQADPTAPVDPGEFLQTLPPSLRQSLLADMEESQISALPADLAAEAQNLRRDYEQRNRQMMHERFFNHVNHTGPSGPTLSSILRNTVNRLGSQYAIHTGAGGPGRSNLWRSIGR-----GGPGHQQSAAALAAANNVKFRGRQLLDHEGLSCLLILLFIDDAKLNTTRLHRILRNLCYHAPTRDWVVKCLLSILEKANTGAECPA--QQTMADPSTSG-ANTPTPAK 2655 HSP 3 Score: 119.013 bits (297), Expect = 2.230e-25 Identity = 91/164 (55.49%), Postives = 113/164 (68.90%), Query Frame = 0
Query: 2205 PVIRRNXXXXXXXXXXXXXXXIGRPDSLNNKDDXXXXXXXXXXXXXNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNF------EHDRSGKDKGGLLDRFTKETVVLNAPSK 2362
PVI+R+SLLTDKLLRLLSLIS+G+PD L D+ E G + + D ++LAV+VLTSK+CSEEGLEDVTALLLNLS+G TR+ IL LLL GA++LGNVV A+V DL EL +K++ E + GK KG + DRFTKE VVL AP+K
Sbjct: 3178 PVIKRSSLLTDKLLRLLSLISLGQPDVLKRLDESARQEENTEGGIL--VDKAVKEDQIQLAVEVLTSKACSEEGLEDVTALLLNLSYGGTQTRESILHLLLAGARQLGNVVSAHVSDLLTELAELKASGGLASLPKEEEEEGKHKGVMADRFTKEAVVLVAPTK 3663 HSP 4 Score: 104.375 bits (259), Expect = 5.315e-21 Identity = 67/163 (41.10%), Postives = 98/163 (60.12%), Query Frame = 0
Query: 1451 PSHPLLMGRQNLETGTGNARNSASTRSLPRTRGNSLRPYFQLNSR-AQNNPSTPAILQNFLGGSNPQELITF----------GFRRSGAPLMMDV-VGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE 1601
PSHPLLMGR G+AR RSL R RG Y QLN R P TPAILQ+ LG ++ ++ + R + L+M+ G ++LD++++++P L+ G +ALS++P+AL+RW EESRV+DG+S+HDC+T KPE+++VIE
Sbjct: 394 PSHPLLMGRTEQVVPAGSARGQG--RSLTRQRGFR---YIQLNPRNGGGGPGTPAILQSLLGHNSGRDFLQLTGNHPGLNVGAMRDATRVLVMESGSGFAILDSLEDEIPGLEGLGQGG--GSALSTVPNALVRWTEESRVLDGDSLHDCMTVCKPELLDVIE 861
BLAST of EMLSAG00000004991 vs. C. finmarchicus
Match: gi|592944258|gb|GAXK01014295.1| (TSA: Calanus finmarchicus comp8631_c3_seq7 transcribed RNA sequence) HSP 1 Score: 846.655 bits (2186), Expect = 0.000e+0 Identity = 413/505 (81.78%), Postives = 448/505 (88.71%), Query Frame = 0
Query: 2453 LGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMEXXXXXXXXXXXXXXPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTXXXXXXXXXXXXXXAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
L +CLK+LA+TPDHHAVLVLQ +VEAFFLVHAA +EKKK QKETR+EQLAHI+E+ E E + S +SPDTKKFLAFAETHRTVLNQILRQST HLADGPFSVLVDHTRVLDFDIKRRYFRTELER+DE +RREDLAVHVRR+ VFEESFREL RRS EE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNP YALFK+SPGD+VTY I + S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHIL K VK+ DMESEDY FYKGL+FL+ +KVSDLGYDLTFSTEIQEFGVTE+RDLIP+GRN+ VT++ K DYIRLVCQMKMTGAIRKQL+AFLEGFY+IIPRRLI+IFNEQELELLLSGLP I+IDDLK+N+EYHKY SLQI+WFWRALRSFDQTD+AKF+QFVTGSSKVPLQGF LEGMNG QKFQIHRDDRSTDRLP+AHTCFNQLDLPAYETYDKLRTYLLKAI ECSE F
Sbjct: 5555 LSNCLKELADTPDHHAVLVLQPTVEAFFLVHAAVISSEEKKKPNQKETRKEQLAHIEEK-EGQLESEQAPQTVVGSEEDISPDTKKFLAFAETHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERLDEGIRREDLAVHVRRDAVFEESFRELHRRSAEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPNYALFKSSPGDRVTYTINDFSHINSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKLVKHQDMESEDYEFYKGLDFLLENKVSDLGYDLTFSTEIQEFGVTEVRDLIPDGRNVIVTDQNKADYIRLVCQMKMTGAIRKQLAAFLEGFYDIIPRRLIAIFNEQELELLLSGLPDINIDDLKANSEYHKYSVTSLQIVWFWRALRSFDQTDRAKFLQFVTGSSKVPLQGFGALEGMNGAQKFQIHRDDRSTDRLPAAHTCFNQLDLPAYETYDKLRTYLLKAIQECSEGFGF 7066 HSP 2 Score: 412.149 bits (1058), Expect = 1.401e-114 Identity = 262/524 (50.00%), Postives = 329/524 (62.79%), Query Frame = 0
Query: 915 CRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV------------------KTPDICE-SSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKDMKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSAR---KSKNDVPDSSAEDXXXXXXXXXXXXVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERDGSD----NFTIIP--QVGSN------VNSDQDHHQDGSRFAGH-GCQDDVDRAXESSQ 1403
CR P F SVA+DILR + S L+KRGE ++D RLLVKSL KA ++ APPL +VS+SVI DLL+FLV K+ DI E ++ A+++ S T P V+R + + +PG + + K+ L KSAVCRLLAE+VKSY GCA+LIT+HVY++GI + +KED +ALAF+LDELLTS DK+ LVK L+AALASCNH PEAQ++LV EVK AL R+L PE +KH+K+Q+LT LISTMIE+CP+S+ T QP FK Q MNNIVK M+KRGLITDLARV HALDLSSP++A T+N+ALKPLETLSRIVNQPT L+ + K KND ++ A D + N TNTTNSEATRAQ DE D EATEHD+STTAES+DPNSESQL TVE+ + ED+DEMM+QLLE+D D +I + G N +N Q Q+ S G G ++D D SQ
Sbjct: 2 CRQPDTFTSVARDILRVDISLLSKRGEPEDDHRLLVKSLLGKA-SAPAPPLKDVSKSVISDLLDFLVQSDPDEPLESDQVGDIIGKSDDIAELPTSVASILNSGTVAVQGSSTRGPAVVRQISSELIPG--EKKEEVVKDGKETKEVEDARKKRHLLPKSAVCRLLAEMVKSYAGCARLITEHVYQAGITDLVKEDCTALAFILDELLTSTKDKESATLVKMLVAALASCNHAPEAQTSLVTEVKNALTRALSLPECSMKHTKVQSLTGLISTMIENCPASL-ATNQPPFKSG-QVNMNNIVKCMVKRGLITDLARVSHALDLSSPSMAATVNAALKPLETLSRIVNQPTGLLPPTQNKTKPKND--EARANDINNGN-----------------------TNTTNSEATRAQNDEIAGIDNEATEHDISTTAESMDPNSESQLHTVEEGDGEDFDEMMEQLLEQDRGDAALLEVAVIARGETGQNMETDDTINDSQMMSQEESFVEGEGGAEEDSDTDSSHSQ 1483 HSP 3 Score: 327.02 bits (837), Expect = 1.785e-88 Identity = 197/331 (59.52%), Postives = 245/331 (74.02%), Query Frame = 0
Query: 1877 PIPSTSGTS--QNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS---NVPEVNPEFLAALPPNIQEEVLAXXX-XXXXXXXXXVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMXXXXXXXXXXXQSLRREXEQRNRNFMHERFFNN------GGNSLSSILRGSVSRLGNQYIIGNLVG---REAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVK 2192
P+P +SGTS ++PD+SSILGD++IPEGVDPSFLAALPE+MR EVI++QRRL RQ+ Q G+ + EVNPEFLAALPPNIQEEVLAQQR +Q++ +Q +P APVDPGEF QTLP SLRQS+LADME+SQ++ALPA+LAAEAQ+LRR+ EQRNR MHERFFN+ G +LSSILR +V+RLG+QY I G R S R ++ AN+++F+GRQLLD+EGLSCLLILLF+DD+K+NTTRLHRILRNLCYHAPTRDWVV+CL+SILE+AN + A Q + P+ S ++T TP K
Sbjct: 3134 PLPGSSGTSTIRDPDFSSILGDLDIPEGVDPSFLAALPEDMRAEVIEEQRRLVRARQQPPAQPPPGTTAQGMQEVNPEFLAALPPNIQEEVLAQQRLEQQRQNAAQADPTAPVDPGEFLQTLPPSLRQSLLADMEESQISALPADLAAEAQNLRRDYEQRNRQMMHERFFNHVNHTGPSGPTLSSILRNTVNRLGSQYAIHTGAGGPGRSNLWRSIGR-----GGPGHQQSAAALAAANNVKFRGRQLLDHEGLSCLLILLFIDDAKLNTTRLHRILRNLCYHAPTRDWVVKCLLSILEKANTGAECPA--QQTMADPSTSG-ANTPTPAK 4102 HSP 4 Score: 119.013 bits (297), Expect = 2.517e-25 Identity = 91/164 (55.49%), Postives = 113/164 (68.90%), Query Frame = 0
Query: 2205 PVIRRNXXXXXXXXXXXXXXXIGRPDSLNNKDDXXXXXXXXXXXXXNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNF------EHDRSGKDKGGLLDRFTKETVVLNAPSK 2362
PVI+R+SLLTDKLLRLLSLIS+G+PD L D+ E G + + D ++LAV+VLTSK+CSEEGLEDVTALLLNLS+G TR+ IL LLL GA++LGNVV A+V DL EL +K++ E + GK KG + DRFTKE VVL AP+K
Sbjct: 4625 PVIKRSSLLTDKLLRLLSLISLGQPDVLKRLDESARQEENTEGGIL--VDKAVKEDQIQLAVEVLTSKACSEEGLEDVTALLLNLSYGGTQTRESILHLLLAGARQLGNVVSAHVSDLLTELAELKASGGLASLPKEEEEEGKHKGVMADRFTKEAVVLVAPTK 5110 HSP 5 Score: 105.145 bits (261), Expect = 4.030e-21 Identity = 68/163 (41.72%), Postives = 99/163 (60.74%), Query Frame = 0
Query: 1451 PSHPLLMGRQNLETGTGNARNSASTRSLPRTRGNSLRPYFQLNSR-AQNNPSTPAILQNFLGGSNPQELITF----------GFRRSGAPLMMDV-VGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE 1601
PSHPLLMGR G+AR RSL R RG R Y QLN R P TPAILQ+ LG ++ ++ + R + L+M+ G ++LD++++++P L+ G +ALS++P+AL+RW EESRV+DG+S+HDC+T KPE+++VIE
Sbjct: 1841 PSHPLLMGRTEQVVPAGSARGQG--RSLTRQRG--FR-YIQLNPRNGGGGPGTPAILQSLLGHNSGRDFLQLTGNHPGLNVGAMRDATRVLVMESGSGFAILDSLEDEIPGLEGLGQGG--GSALSTVPNALVRWTEESRVLDGDSLHDCMTVCKPELLDVIE 2308
BLAST of EMLSAG00000004991 vs. C. finmarchicus
Match: gi|592944259|gb|GAXK01014294.1| (TSA: Calanus finmarchicus comp8631_c3_seq6 transcribed RNA sequence) HSP 1 Score: 847.425 bits (2188), Expect = 0.000e+0 Identity = 413/505 (81.78%), Postives = 448/505 (88.71%), Query Frame = 0
Query: 2453 LGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMEXXXXXXXXXXXXXXPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTXXXXXXXXXXXXXXAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
L +CLK+LA+TPDHHAVLVLQ +VEAFFLVHAA +EKKK QKETR+EQLAHI+E+ E E + S +SPDTKKFLAFAETHRTVLNQILRQST HLADGPFSVLVDHTRVLDFDIKRRYFRTELER+DE +RREDLAVHVRR+ VFEESFREL RRS EE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNP YALFK+SPGD+VTY I + S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHIL K VK+ DMESEDY FYKGL+FL+ +KVSDLGYDLTFSTEIQEFGVTE+RDLIP+GRN+ VT++ K DYIRLVCQMKMTGAIRKQL+AFLEGFY+IIPRRLI+IFNEQELELLLSGLP I+IDDLK+N+EYHKY SLQI+WFWRALRSFDQTD+AKF+QFVTGSSKVPLQGF LEGMNG QKFQIHRDDRSTDRLP+AHTCFNQLDLPAYETYDKLRTYLLKAI ECSE F
Sbjct: 6047 LSNCLKELADTPDHHAVLVLQPTVEAFFLVHAAVISSEEKKKPNQKETRKEQLAHIEEK-EGQLESEQAPQTVVGSEEDISPDTKKFLAFAETHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERLDEGIRREDLAVHVRRDAVFEESFRELHRRSAEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPNYALFKSSPGDRVTYTINDFSHINSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKLVKHQDMESEDYEFYKGLDFLLENKVSDLGYDLTFSTEIQEFGVTEVRDLIPDGRNVIVTDQNKADYIRLVCQMKMTGAIRKQLAAFLEGFYDIIPRRLIAIFNEQELELLLSGLPDINIDDLKANSEYHKYSVTSLQIVWFWRALRSFDQTDRAKFLQFVTGSSKVPLQGFGALEGMNGAQKFQIHRDDRSTDRLPAAHTCFNQLDLPAYETYDKLRTYLLKAIQECSEGFGF 7558 HSP 2 Score: 535.413 bits (1378), Expect = 1.802e-152 Identity = 337/644 (52.33%), Postives = 424/644 (65.84%), Query Frame = 0
Query: 796 GLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTS-KEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV------------------KTPDICE-SSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKDMKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSAR---KSKNDVPDSSAEDXXXXXXXXXXXXVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERDGSD----NFTIIP--QVGSN------VNSDQDHHQDGSRFAGH-GCQDDVDRAXESSQ 1403
GL +D+ ALL+A VG +++PV+ DALNA+LRLCLRLT+ F QA FA LGG+KLLL L Q+S FSG +SLA+LLVRHVLED TL+HTMEK+IR+S S + KE++Y+LR LAPAACR P F SVA+DILR + S L+KRGE ++D RLLVKSL KA ++ APPL +VS+SVI DLL+FLV K+ DI E ++ A+++ S T P V+R + + +PG + + K+ L KSAVCRLLAE+VKSY GCA+LIT+HVY++GI + +KED +ALAF+LDELLTS DK+ LVK L+AALASCNH PEAQ++LV EVK AL R+L PE +KH+K+Q+LT LISTMIE+CP+S+ T QP FK Q MNNIVK M+KRGLITDLARV HALDLSSP++A T+N+ALKPLETLSRIVNQPT L+ + K KND ++ A D + N TNTTNSEATRAQ DE D EATEHD+STTAES+DPNSESQL TVE+ + ED+DEMM+QLLE+D D +I + G N +N Q Q+ S G G ++D D SQ
Sbjct: 134 GLSSDQSQALLRAAVGLIAVPVEPDALNAILRLCLRLTRTFSQATLFAELGGIKLLLGLKQASSFSGFSSLASLLVRHVLEDDLTLKHTMEKIIRSSAATSTAATTKELHYLLRSLAPAACRQPDTFTSVARDILRVDISLLSKRGEPEDDHRLLVKSLLGKA-SAPAPPLKDVSKSVISDLLDFLVQSDPDEPLESDQVGDIIGKSDDIAELPTSVASILNSGTVAVQGSSTRGPAVVRQISSELIPG--EKKEEVVKDGKETKEVEDARKKRHLLPKSAVCRLLAEMVKSYAGCARLITEHVYQAGITDLVKEDCTALAFILDELLTSTKDKESATLVKMLVAALASCNHAPEAQTSLVTEVKNALTRALSLPECSMKHTKVQSLTGLISTMIENCPASL-ATNQPPFKSG-QVNMNNIVKCMVKRGLITDLARVSHALDLSSPSMAATVNAALKPLETLSRIVNQPTGLLPPTQNKTKPKND--EARANDINNGN-----------------------TNTTNSEATRAQNDEIAGIDNEATEHDISTTAESMDPNSESQLHTVEEGDGEDFDEMMEQLLEQDRGDAALLEVAVIARGETGQNMETDDTINDSQMMSQEESFVEGEGGAEEDSDTDSSHSQ 1975 HSP 3 Score: 327.405 bits (838), Expect = 1.386e-88 Identity = 197/331 (59.52%), Postives = 245/331 (74.02%), Query Frame = 0
Query: 1877 PIPSTSGTS--QNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS---NVPEVNPEFLAALPPNIQEEVLAXXX-XXXXXXXXXVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMXXXXXXXXXXXQSLRREXEQRNRNFMHERFFNN------GGNSLSSILRGSVSRLGNQYIIGNLVG---REAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVK 2192
P+P +SGTS ++PD+SSILGD++IPEGVDPSFLAALPE+MR EVI++QRRL RQ+ Q G+ + EVNPEFLAALPPNIQEEVLAQQR +Q++ +Q +P APVDPGEF QTLP SLRQS+LADME+SQ++ALPA+LAAEAQ+LRR+ EQRNR MHERFFN+ G +LSSILR +V+RLG+QY I G R S R ++ AN+++F+GRQLLD+EGLSCLLILLF+DD+K+NTTRLHRILRNLCYHAPTRDWVV+CL+SILE+AN + A Q + P+ S ++T TP K
Sbjct: 3626 PLPGSSGTSTIRDPDFSSILGDLDIPEGVDPSFLAALPEDMRAEVIEEQRRLVRARQQPPAQPPPGTTAQGMQEVNPEFLAALPPNIQEEVLAQQRLEQQRQNAAQADPTAPVDPGEFLQTLPPSLRQSLLADMEESQISALPADLAAEAQNLRRDYEQRNRQMMHERFFNHVNHTGPSGPTLSSILRNTVNRLGSQYAIHTGAGGPGRSNLWRSIGR-----GGPGHQQSAAALAAANNVKFRGRQLLDHEGLSCLLILLFIDDAKLNTTRLHRILRNLCYHAPTRDWVVKCLLSILEKANTGAECPA--QQTMADPSTSG-ANTPTPAK 4594 HSP 4 Score: 119.013 bits (297), Expect = 2.221e-25 Identity = 91/164 (55.49%), Postives = 113/164 (68.90%), Query Frame = 0
Query: 2205 PVIRRNXXXXXXXXXXXXXXXIGRPDSLNNKDDXXXXXXXXXXXXXNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNF------EHDRSGKDKGGLLDRFTKETVVLNAPSK 2362
PVI+R+SLLTDKLLRLLSLIS+G+PD L D+ E G + + D ++LAV+VLTSK+CSEEGLEDVTALLLNLS+G TR+ IL LLL GA++LGNVV A+V DL EL +K++ E + GK KG + DRFTKE VVL AP+K
Sbjct: 5117 PVIKRSSLLTDKLLRLLSLISLGQPDVLKRLDESARQEENTEGGIL--VDKAVKEDQIQLAVEVLTSKACSEEGLEDVTALLLNLSYGGTQTRESILHLLLAGARQLGNVVSAHVSDLLTELAELKASGGLASLPKEEEEEGKHKGVMADRFTKEAVVLVAPTK 5602 HSP 5 Score: 105.145 bits (261), Expect = 3.486e-21 Identity = 67/163 (41.10%), Postives = 98/163 (60.12%), Query Frame = 0
Query: 1451 PSHPLLMGRQNLETGTGNARNSASTRSLPRTRGNSLRPYFQLNSR-AQNNPSTPAILQNFLGGSNPQELITF----------GFRRSGAPLMMDV-VGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE 1601
PSHPLLMGR G+AR RSL R RG Y QLN R P TPAILQ+ LG ++ ++ + R + L+M+ G ++LD++++++P L+ G +ALS++P+AL+RW EESRV+DG+S+HDC+T KPE+++VIE
Sbjct: 2333 PSHPLLMGRTEQVVPAGSARGQG--RSLTRQRGFR---YIQLNPRNGGGGPGTPAILQSLLGHNSGRDFLQLTGNHPGLNVGAMRDATRVLVMESGSGFAILDSLEDEIPGLEGLGQGG--GSALSTVPNALVRWTEESRVLDGDSLHDCMTVCKPELLDVIE 2800
BLAST of EMLSAG00000004991 vs. C. finmarchicus
Match: gi|592944260|gb|GAXK01014293.1| (TSA: Calanus finmarchicus comp8631_c3_seq5 transcribed RNA sequence) HSP 1 Score: 846.655 bits (2186), Expect = 0.000e+0 Identity = 413/505 (81.78%), Postives = 448/505 (88.71%), Query Frame = 0
Query: 2453 LGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMEXXXXXXXXXXXXXXPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTXXXXXXXXXXXXXXAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
L +CLK+LA+TPDHHAVLVLQ +VEAFFLVHAA +EKKK QKETR+EQLAHI+E+ E E + S +SPDTKKFLAFAETHRTVLNQILRQST HLADGPFSVLVDHTRVLDFDIKRRYFRTELER+DE +RREDLAVHVRR+ VFEESFREL RRS EE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNP YALFK+SPGD+VTY I + S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHIL K VK+ DMESEDY FYKGL+FL+ +KVSDLGYDLTFSTEIQEFGVTE+RDLIP+GRN+ VT++ K DYIRLVCQMKMTGAIRKQL+AFLEGFY+IIPRRLI+IFNEQELELLLSGLP I+IDDLK+N+EYHKY SLQI+WFWRALRSFDQTD+AKF+QFVTGSSKVPLQGF LEGMNG QKFQIHRDDRSTDRLP+AHTCFNQLDLPAYETYDKLRTYLLKAI ECSE F
Sbjct: 6454 LSNCLKELADTPDHHAVLVLQPTVEAFFLVHAAVISSEEKKKPNQKETRKEQLAHIEEK-EGQLESEQAPQTVVGSEEDISPDTKKFLAFAETHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERLDEGIRREDLAVHVRRDAVFEESFRELHRRSAEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPNYALFKSSPGDRVTYTINDFSHINSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKLVKHQDMESEDYEFYKGLDFLLENKVSDLGYDLTFSTEIQEFGVTEVRDLIPDGRNVIVTDQNKADYIRLVCQMKMTGAIRKQLAAFLEGFYDIIPRRLIAIFNEQELELLLSGLPDINIDDLKANSEYHKYSVTSLQIVWFWRALRSFDQTDRAKFLQFVTGSSKVPLQGFGALEGMNGAQKFQIHRDDRSTDRLPAAHTCFNQLDLPAYETYDKLRTYLLKAIQECSEGFGF 7965 HSP 2 Score: 657.136 bits (1694), Expect = 0.000e+0 Identity = 406/824 (49.27%), Postives = 518/824 (62.86%), Query Frame = 0
Query: 659 DLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK--SKGSRDAVVK------------------------------XXXXXXXXXXXMELGEEEKKVSDPNEEDVITTIPT-----------AEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTS-KEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV------------------KTPDICE-SSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKDMKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSAR---KSKNDVPDSSAEDXXXXXXXXXXXXVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERDGSD----NFTIIP--QVGSN------VNSDQDHHQDGSRFAGH-GCQDDVDRAXESSQ 1403
DLYEKV+L M RR ++ S+CS WKW+D+ +G+W Y P +NKTI+DAFW+GE SV+ TTGRRKY IQFG M+QANEETGNRRP+MI++K K + AV + S + N E + E +D +++ I +I GL +D+ ALL+A VG +++PV+ DALNA+LRLCLRLT+ F QA FA LGG+KLLL L Q+S FSG +SLA+LLVRHVLED TL+HTMEK+IR+S S + KE++Y+LR LAPAACR P F SVA+DILR + S L+KRGE ++D RLLVKSL KA ++ APPL +VS+SVI DLL+FLV K+ DI E ++ A+++ S T P V+R + + +PG + + K+ L KSAVCRLLAE+VKSY GCA+LIT+HVY++GI + +KED +ALAF+LDELLTS DK+ LVK L+AALASCNH PEAQ++LV EVK AL R+L PE +KH+K+Q+LT LISTMIE+CP+S+ T QP FK Q MNNIVK M+KRGLITDLARV HALDLSSP++A T+N+ALKPLETLSRIVNQPT L+ + K KND ++ A D + N TNTTNSEATRAQ DE D EATEHD+STTAES+DPNSESQL TVE+ + ED+DEMM+QLLE+D D +I + G N +N Q Q+ S G G ++D D SQ
Sbjct: 1 DLYEKVVLAMKRRDAMASICSHSWKWFDVGAGKWQGYQPGNNKTINDAFWAGEPSVKFTTGRRKYNIQFGAMMQANEETGNRRPLMITLKRDEKEADTAVERRGRWKKEDSAAAPGEKKEEEDKDESENTTTESTAAPVEANTAEPEKMETSQTDAEKQETIDSIAAKSTPPPHTTYKCSGLSSDQSQALLRAAVGLIAVPVEPDALNAILRLCLRLTRTFSQATLFAELGGIKLLLGLKQASSFSGFSSLASLLVRHVLEDDLTLKHTMEKIIRSSAATSTAATTKELHYLLRSLAPAACRQPDTFTSVARDILRVDISLLSKRGEPEDDHRLLVKSLLGKA-SAPAPPLKDVSKSVISDLLDFLVQSDPDEPLESDQVGDIIGKSDDIAELPTSVASILNSGTVAVQGSSTRGPAVVRQISSELIPG--EKKEEVVKDGKETKEVEDARKKRHLLPKSAVCRLLAEMVKSYAGCARLITEHVYQAGITDLVKEDCTALAFILDELLTSTKDKESATLVKMLVAALASCNHAPEAQTSLVTEVKNALTRALSLPECSMKHTKVQSLTGLISTMIENCPASL-ATNQPPFKSG-QVNMNNIVKCMVKRGLITDLARVSHALDLSSPSMAATVNAALKPLETLSRIVNQPTGLLPPTQNKTKPKND--EARANDINNGN-----------------------TNTTNSEATRAQNDEIAGIDNEATEHDISTTAESMDPNSESQLHTVEEGDGEDFDEMMEQLLEQDRGDAALLEVAVIARGETGQNMETDDTINDSQMMSQEESFVEGEGGAEEDSDTDSSHSQ 2382 HSP 3 Score: 327.405 bits (838), Expect = 1.658e-88 Identity = 197/331 (59.52%), Postives = 245/331 (74.02%), Query Frame = 0
Query: 1877 PIPSTSGTS--QNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS---NVPEVNPEFLAALPPNIQEEVLAXXX-XXXXXXXXXVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMXXXXXXXXXXXQSLRREXEQRNRNFMHERFFNN------GGNSLSSILRGSVSRLGNQYIIGNLVG---REAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVK 2192
P+P +SGTS ++PD+SSILGD++IPEGVDPSFLAALPE+MR EVI++QRRL RQ+ Q G+ + EVNPEFLAALPPNIQEEVLAQQR +Q++ +Q +P APVDPGEF QTLP SLRQS+LADME+SQ++ALPA+LAAEAQ+LRR+ EQRNR MHERFFN+ G +LSSILR +V+RLG+QY I G R S R ++ AN+++F+GRQLLD+EGLSCLLILLF+DD+K+NTTRLHRILRNLCYHAPTRDWVV+CL+SILE+AN + A Q + P+ S ++T TP K
Sbjct: 4033 PLPGSSGTSTIRDPDFSSILGDLDIPEGVDPSFLAALPEDMRAEVIEEQRRLVRARQQPPAQPPPGTTAQGMQEVNPEFLAALPPNIQEEVLAQQRLEQQRQNAAQADPTAPVDPGEFLQTLPPSLRQSLLADMEESQISALPADLAAEAQNLRRDYEQRNRQMMHERFFNHVNHTGPSGPTLSSILRNTVNRLGSQYAIHTGAGGPGRSNLWRSIGR-----GGPGHQQSAAALAAANNVKFRGRQLLDHEGLSCLLILLFIDDAKLNTTRLHRILRNLCYHAPTRDWVVKCLLSILEKANTGAECPA--QQTMADPSTSG-ANTPTPAK 5001 HSP 4 Score: 119.013 bits (297), Expect = 2.138e-25 Identity = 91/164 (55.49%), Postives = 113/164 (68.90%), Query Frame = 0
Query: 2205 PVIRRNXXXXXXXXXXXXXXXIGRPDSLNNKDDXXXXXXXXXXXXXNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNF------EHDRSGKDKGGLLDRFTKETVVLNAPSK 2362
PVI+R+SLLTDKLLRLLSLIS+G+PD L D+ E G + + D ++LAV+VLTSK+CSEEGLEDVTALLLNLS+G TR+ IL LLL GA++LGNVV A+V DL EL +K++ E + GK KG + DRFTKE VVL AP+K
Sbjct: 5524 PVIKRSSLLTDKLLRLLSLISLGQPDVLKRLDESARQEENTEGGIL--VDKAVKEDQIQLAVEVLTSKACSEEGLEDVTALLLNLSYGGTQTRESILHLLLAGARQLGNVVSAHVSDLLTELAELKASGGLASLPKEEEEEGKHKGVMADRFTKEAVVLVAPTK 6009 HSP 5 Score: 105.145 bits (261), Expect = 3.706e-21 Identity = 67/163 (41.10%), Postives = 98/163 (60.12%), Query Frame = 0
Query: 1451 PSHPLLMGRQNLETGTGNARNSASTRSLPRTRGNSLRPYFQLNSR-AQNNPSTPAILQNFLGGSNPQELITF----------GFRRSGAPLMMDV-VGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE 1601
PSHPLLMGR G+AR RSL R RG Y QLN R P TPAILQ+ LG ++ ++ + R + L+M+ G ++LD++++++P L+ G +ALS++P+AL+RW EESRV+DG+S+HDC+T KPE+++VIE
Sbjct: 2740 PSHPLLMGRTEQVVPAGSARGQG--RSLTRQRGFR---YIQLNPRNGGGGPGTPAILQSLLGHNSGRDFLQLTGNHPGLNVGAMRDATRVLVMESGSGFAILDSLEDEIPGLEGLGQGG--GSALSTVPNALVRWTEESRVLDGDSLHDCMTVCKPELLDVIE 3207
BLAST of EMLSAG00000004991 vs. C. finmarchicus
Match: gi|592944261|gb|GAXK01014292.1| (TSA: Calanus finmarchicus comp8631_c3_seq4 transcribed RNA sequence) HSP 1 Score: 845.884 bits (2184), Expect = 0.000e+0 Identity = 413/505 (81.78%), Postives = 448/505 (88.71%), Query Frame = 0
Query: 2453 LGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMEXXXXXXXXXXXXXXPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTXXXXXXXXXXXXXXAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
L +CLK+LA+TPDHHAVLVLQ +VEAFFLVHAA +EKKK QKETR+EQLAHI+E+ E E + S +SPDTKKFLAFAETHRTVLNQILRQST HLADGPFSVLVDHTRVLDFDIKRRYFRTELER+DE +RREDLAVHVRR+ VFEESFREL RRS EE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNP YALFK+SPGD+VTY I + S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHIL K VK+ DMESEDY FYKGL+FL+ +KVSDLGYDLTFSTEIQEFGVTE+RDLIP+GRN+ VT++ K DYIRLVCQMKMTGAIRKQL+AFLEGFY+IIPRRLI+IFNEQELELLLSGLP I+IDDLK+N+EYHKY SLQI+WFWRALRSFDQTD+AKF+QFVTGSSKVPLQGF LEGMNG QKFQIHRDDRSTDRLP+AHTCFNQLDLPAYETYDKLRTYLLKAI ECSE F
Sbjct: 6715 LSNCLKELADTPDHHAVLVLQPTVEAFFLVHAAVISSEEKKKPNQKETRKEQLAHIEEK-EGQLESEQAPQTVVGSEEDISPDTKKFLAFAETHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERLDEGIRREDLAVHVRRDAVFEESFRELHRRSAEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPNYALFKSSPGDRVTYTINDFSHINSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKLVKHQDMESEDYEFYKGLDFLLENKVSDLGYDLTFSTEIQEFGVTEVRDLIPDGRNVIVTDQNKADYIRLVCQMKMTGAIRKQLAAFLEGFYDIIPRRLIAIFNEQELELLLSGLPDINIDDLKANSEYHKYSVTSLQIVWFWRALRSFDQTDRAKFLQFVTGSSKVPLQGFGALEGMNGAQKFQIHRDDRSTDRLPAAHTCFNQLDLPAYETYDKLRTYLLKAIQECSEGFGF 8226 HSP 2 Score: 729.169 bits (1881), Expect = 0.000e+0 Identity = 445/901 (49.39%), Postives = 570/901 (63.26%), Query Frame = 0
Query: 584 EEDVFRTTESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCSGSVG--TPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK--SKGSRDAVVK------------------------------XXXXXXXXXXXMELGEEEKKVSDPNEEDVITTIPT-----------AEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTS-KEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV------------------KTPDICE-SSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKDMKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSAR---KSKNDVPDSSAEDXXXXXXXXXXXXVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERDGSD----NFTIIP--QVGSN------VNSDQDHHQDGSRFAGH-GCQDDVDRAXESSQ 1403
EE V ES +AAVRIHLF LLFE+C LC ++VE S + +M L+ V+ + L+ +VG TPKW+TP+LLFIDLYEKV+L M RR ++ S+CS WKW+D+ +G+W Y P +NKTI+DAFW+GE SV+ TTGRRKY IQFG M+QANEETGNRRP+MI++K K + AV + S + N E + E +D +++ I +I GL +D+ ALL+A VG +++PV+ DALNA+LRLCLRLT+ F QA FA LGG+KLLL L Q+S FSG +SLA+LLVRHVLED TL+HTMEK+IR+S S + KE++Y+LR LAPAACR P F SVA+DILR + S L+KRGE ++D RLLVKSL KA ++ APPL +VS+SVI DLL+FLV K+ DI E ++ A+++ S T P V+R + + +PG + + K+ L KSAVCRLLAE+VKSY GCA+LIT+HVY++GI + +KED +ALAF+LDELLTS DK+ LVK L+AALASCNH PEAQ++LV EVK AL R+L PE +KH+K+Q+LT LISTMIE+CP+S+ T QP FK Q MNNIVK M+KRGLITDLARV HALDLSSP++A T+N+ALKPLETLSRIVNQPT L+ + K KND ++ A D + N TNTTNSEATRAQ DE D EATEHD+STTAES+DPNSESQL TVE+ + ED+DEMM+QLLE+D D +I + G N +N Q Q+ S G G ++D D SQ
Sbjct: 34 EEAVKDAPESARAAVRIHLFTLLFEDCSRLCVQLVEGSGAIELMVQLIGVAQEALQGAP-AVGRETPKWITPMLLFIDLYEKVVLAMKRRDAMASICSHSWKWFDVGAGKWQGYQPGNNKTINDAFWAGEPSVKFTTGRRKYNIQFGAMMQANEETGNRRPLMITLKRDEKEADTAVERRGRWKKEDSAAAPGEKKEEEDKDESENTTTESTAAPVEANTAEPEKMETSQTDAEKQETIDSIAAKSTPPPHTTYKCSGLSSDQSQALLRAAVGLIAVPVEPDALNAILRLCLRLTRTFSQATLFAELGGIKLLLGLKQASSFSGFSSLASLLVRHVLEDDLTLKHTMEKIIRSSAATSTAATTKELHYLLRSLAPAACRQPDTFTSVARDILRVDISLLSKRGEPEDDHRLLVKSLLGKA-SAPAPPLKDVSKSVISDLLDFLVQSDPDEPLESDQVGDIIGKSDDIAELPTSVASILNSGTVAVQGSSTRGPAVVRQISSELIPG--EKKEEVVKDGKETKEVEDARKKRHLLPKSAVCRLLAEMVKSYAGCARLITEHVYQAGITDLVKEDCTALAFILDELLTSTKDKESATLVKMLVAALASCNHAPEAQTSLVTEVKNALTRALSLPECSMKHTKVQSLTGLISTMIENCPASL-ATNQPPFKSG-QVNMNNIVKCMVKRGLITDLARVSHALDLSSPSMAATVNAALKPLETLSRIVNQPTGLLPPTQNKTKPKND--EARANDINNGN-----------------------TNTTNSEATRAQNDEIAGIDNEATEHDISTTAESMDPNSESQLHTVEEGDGEDFDEMMEQLLEQDRGDAALLEVAVIARGETGQNMETDDTINDSQMMSQEESFVEGEGGAEEDSDTDSSHSQ 2643 HSP 3 Score: 327.02 bits (837), Expect = 2.237e-88 Identity = 197/331 (59.52%), Postives = 245/331 (74.02%), Query Frame = 0
Query: 1877 PIPSTSGTS--QNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS---NVPEVNPEFLAALPPNIQEEVLAXXX-XXXXXXXXXVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMXXXXXXXXXXXQSLRREXEQRNRNFMHERFFNN------GGNSLSSILRGSVSRLGNQYIIGNLVG---REAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVK 2192
P+P +SGTS ++PD+SSILGD++IPEGVDPSFLAALPE+MR EVI++QRRL RQ+ Q G+ + EVNPEFLAALPPNIQEEVLAQQR +Q++ +Q +P APVDPGEF QTLP SLRQS+LADME+SQ++ALPA+LAAEAQ+LRR+ EQRNR MHERFFN+ G +LSSILR +V+RLG+QY I G R S R ++ AN+++F+GRQLLD+EGLSCLLILLF+DD+K+NTTRLHRILRNLCYHAPTRDWVV+CL+SILE+AN + A Q + P+ S ++T TP K
Sbjct: 4294 PLPGSSGTSTIRDPDFSSILGDLDIPEGVDPSFLAALPEDMRAEVIEEQRRLVRARQQPPAQPPPGTTAQGMQEVNPEFLAALPPNIQEEVLAQQRLEQQRQNAAQADPTAPVDPGEFLQTLPPSLRQSLLADMEESQISALPADLAAEAQNLRRDYEQRNRQMMHERFFNHVNHTGPSGPTLSSILRNTVNRLGSQYAIHTGAGGPGRSNLWRSIGR-----GGPGHQQSAAALAAANNVKFRGRQLLDHEGLSCLLILLFIDDAKLNTTRLHRILRNLCYHAPTRDWVVKCLLSILEKANTGAECPA--QQTMADPSTSG-ANTPTPAK 5262 HSP 4 Score: 119.013 bits (297), Expect = 2.154e-25 Identity = 91/164 (55.49%), Postives = 113/164 (68.90%), Query Frame = 0
Query: 2205 PVIRRNXXXXXXXXXXXXXXXIGRPDSLNNKDDXXXXXXXXXXXXXNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNF------EHDRSGKDKGGLLDRFTKETVVLNAPSK 2362
PVI+R+SLLTDKLLRLLSLIS+G+PD L D+ E G + + D ++LAV+VLTSK+CSEEGLEDVTALLLNLS+G TR+ IL LLL GA++LGNVV A+V DL EL +K++ E + GK KG + DRFTKE VVL AP+K
Sbjct: 5785 PVIKRSSLLTDKLLRLLSLISLGQPDVLKRLDESARQEENTEGGIL--VDKAVKEDQIQLAVEVLTSKACSEEGLEDVTALLLNLSYGGTQTRESILHLLLAGARQLGNVVSAHVSDLLTELAELKASGGLASLPKEEEEEGKHKGVMADRFTKEAVVLVAPTK 6270 HSP 5 Score: 105.145 bits (261), Expect = 3.922e-21 Identity = 67/163 (41.10%), Postives = 98/163 (60.12%), Query Frame = 0
Query: 1451 PSHPLLMGRQNLETGTGNARNSASTRSLPRTRGNSLRPYFQLNSR-AQNNPSTPAILQNFLGGSNPQELITF----------GFRRSGAPLMMDV-VGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE 1601
PSHPLLMGR G+AR RSL R RG Y QLN R P TPAILQ+ LG ++ ++ + R + L+M+ G ++LD++++++P L+ G +ALS++P+AL+RW EESRV+DG+S+HDC+T KPE+++VIE
Sbjct: 3001 PSHPLLMGRTEQVVPAGSARGQG--RSLTRQRGFR---YIQLNPRNGGGGPGTPAILQSLLGHNSGRDFLQLTGNHPGLNVGAMRDATRVLVMESGSGFAILDSLEDEIPGLEGLGQGG--GSALSTVPNALVRWTEESRVLDGDSLHDCMTVCKPELLDVIE 3468
BLAST of EMLSAG00000004991 vs. C. finmarchicus
Match: gi|592944262|gb|GAXK01014291.1| (TSA: Calanus finmarchicus comp8631_c3_seq3 transcribed RNA sequence) HSP 1 Score: 846.269 bits (2185), Expect = 0.000e+0 Identity = 413/505 (81.78%), Postives = 448/505 (88.71%), Query Frame = 0
Query: 2453 LGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMEXXXXXXXXXXXXXXPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTXXXXXXXXXXXXXXAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
L +CLK+LA+TPDHHAVLVLQ +VEAFFLVHAA +EKKK QKETR+EQLAHI+E+ E E + S +SPDTKKFLAFAETHRTVLNQILRQST HLADGPFSVLVDHTRVLDFDIKRRYFRTELER+DE +RREDLAVHVRR+ VFEESFREL RRS EE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNP YALFK+SPGD+VTY I + S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHIL K VK+ DMESEDY FYKGL+FL+ +KVSDLGYDLTFSTEIQEFGVTE+RDLIP+GRN+ VT++ K DYIRLVCQMKMTGAIRKQL+AFLEGFY+IIPRRLI+IFNEQELELLLSGLP I+IDDLK+N+EYHKY SLQI+WFWRALRSFDQTD+AKF+QFVTGSSKVPLQGF LEGMNG QKFQIHRDDRSTDRLP+AHTCFNQLDLPAYETYDKLRTYLLKAI ECSE F
Sbjct: 6863 LSNCLKELADTPDHHAVLVLQPTVEAFFLVHAAVISSEEKKKPNQKETRKEQLAHIEEK-EGQLESEQAPQTVVGSEEDISPDTKKFLAFAETHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERLDEGIRREDLAVHVRRDAVFEESFRELHRRSAEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPNYALFKSSPGDRVTYTINDFSHINSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKLVKHQDMESEDYEFYKGLDFLLENKVSDLGYDLTFSTEIQEFGVTEVRDLIPDGRNVIVTDQNKADYIRLVCQMKMTGAIRKQLAAFLEGFYDIIPRRLIAIFNEQELELLLSGLPDINIDDLKANSEYHKYSVTSLQIVWFWRALRSFDQTDRAKFLQFVTGSSKVPLQGFGALEGMNGAQKFQIHRDDRSTDRLPAAHTCFNQLDLPAYETYDKLRTYLLKAIQECSEGFGF 8374 HSP 2 Score: 788.875 bits (2036), Expect = 0.000e+0 Identity = 480/968 (49.59%), Postives = 615/968 (63.53%), Query Frame = 0
Query: 517 ALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIXXXXXXXXXXXXSQSPIEEDVFRTTESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCSGSVG--TPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK--SKGSRDAVVK------------------------------XXXXXXXXXXXMELGEEEKKVSDPNEEDVITTIPT-----------AEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTS-KEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV------------------KTPDICE-SSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKDMKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSAR---KSKNDVPDSSAEDXXXXXXXXXXXXVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERDGSD----NFTIIP--QVGSN------VNSDQDHHQDGSRFAGH-GCQDDVDRAXESSQ 1403
AL GCL L+DTLPETV+R DLL+A+F RNGKEFKEK+L L+ EVK +++ LL EE V ES +AAVRIHLF LLFE+C LC ++VE S + +M L+ V+ + L+ + +VG TPKW+TP+LLFIDLYEKV+L M RR ++ S+CS WKW+D+ +G+W Y P +NKTI+DAFW+GE SV+ TTGRRKY IQFG M+QANEETGNRRP+MI++K K + AV + S + N E + E +D +++ I +I GL +D+ ALL+A VG +++PV+ DALNA+LRLCLRLT+ F QA FA LGG+KLLL L Q+S FSG +SLA+LLVRHVLED TL+HTMEK+IR+S S + KE++Y+LR LAPAACR P F SVA+DILR + S L+KRGE ++D RLLVKSL KA ++ APPL +VS+SVI DLL+FLV K+ DI E ++ A+++ S T P V+R + + +PG + + K+ L KSAVCRLLAE+VKSY GCA+LIT+HVY++GI + +KED +ALAF+LDELLTS DK+ LVK L+AALASCNH PEAQ++LV EVK AL R+L PE +KH+K+Q+LT LISTMIE+CP+S+ T QP FK Q MNNIVK M+KRGLITDLARV HALDLSSP++A T+N+ALKPLETLSRIVNQPT L+ + K KND ++ A D + N TNTTNSEATRAQ DE D EATEHD+STTAES+DPNSESQL TVE+ + ED+DEMM+QLLE+D D +I + G N +N Q Q+ S G G ++D D SQ
Sbjct: 2 ALTGCLTLLDTLPETVYRVTDLLMAIFNRNGKEFKEKLLTQLMGEVKKSVEDLLAVAHSG-------EEAVKDAPESARAAVRIHLFTLLFEDCSRLCVQLVEGSGAIELMVQLIGVAQEALQ-GAPAVGRETPKWITPMLLFIDLYEKVVLAMKRRDAMASICSHSWKWFDVGAGKWQGYQPGNNKTINDAFWAGEPSVKFTTGRRKYNIQFGAMMQANEETGNRRPLMITLKRDEKEADTAVERRGRWKKEDSAAAPGEKKEEEDKDESENTTTESTAAPVEANTAEPEKMETSQTDAEKQETIDSIAAKSTPPPHTTYKCSGLSSDQSQALLRAAVGLIAVPVEPDALNAILRLCLRLTRTFSQATLFAELGGIKLLLGLKQASSFSGFSSLASLLVRHVLEDDLTLKHTMEKIIRSSAATSTAATTKELHYLLRSLAPAACRQPDTFTSVARDILRVDISLLSKRGEPEDDHRLLVKSLLGKA-SAPAPPLKDVSKSVISDLLDFLVQSDPDEPLESDQVGDIIGKSDDIAELPTSVASILNSGTVAVQGSSTRGPAVVRQISSELIPG--EKKEEVVKDGKETKEVEDARKKRHLLPKSAVCRLLAEMVKSYAGCARLITEHVYQAGITDLVKEDCTALAFILDELLTSTKDKESATLVKMLVAALASCNHAPEAQTSLVTEVKNALTRALSLPECSMKHTKVQSLTGLISTMIENCPASL-ATNQPPFKSG-QVNMNNIVKCMVKRGLITDLARVSHALDLSSPSMAATVNAALKPLETLSRIVNQPTGLLPPTQNKTKPKND--EARANDINNGN-----------------------TNTTNSEATRAQNDEIAGIDNEATEHDISTTAESMDPNSESQLHTVEEGDGEDFDEMMEQLLEQDRGDAALLEVAVIARGETGQNMETDDTINDSQMMSQEESFVEGEGGAEEDSDTDSSHSQ 2791 HSP 3 Score: 326.635 bits (836), Expect = 2.547e-88 Identity = 197/331 (59.52%), Postives = 245/331 (74.02%), Query Frame = 0
Query: 1877 PIPSTSGTS--QNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS---NVPEVNPEFLAALPPNIQEEVLAXXX-XXXXXXXXXVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMXXXXXXXXXXXQSLRREXEQRNRNFMHERFFNN------GGNSLSSILRGSVSRLGNQYIIGNLVG---REAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVK 2192
P+P +SGTS ++PD+SSILGD++IPEGVDPSFLAALPE+MR EVI++QRRL RQ+ Q G+ + EVNPEFLAALPPNIQEEVLAQQR +Q++ +Q +P APVDPGEF QTLP SLRQS+LADME+SQ++ALPA+LAAEAQ+LRR+ EQRNR MHERFFN+ G +LSSILR +V+RLG+QY I G R S R ++ AN+++F+GRQLLD+EGLSCLLILLF+DD+K+NTTRLHRILRNLCYHAPTRDWVV+CL+SILE+AN + A Q + P+ S ++T TP K
Sbjct: 4442 PLPGSSGTSTIRDPDFSSILGDLDIPEGVDPSFLAALPEDMRAEVIEEQRRLVRARQQPPAQPPPGTTAQGMQEVNPEFLAALPPNIQEEVLAQQRLEQQRQNAAQADPTAPVDPGEFLQTLPPSLRQSLLADMEESQISALPADLAAEAQNLRRDYEQRNRQMMHERFFNHVNHTGPSGPTLSSILRNTVNRLGSQYAIHTGAGGPGRSNLWRSIGR-----GGPGHQQSAAALAAANNVKFRGRQLLDHEGLSCLLILLFIDDAKLNTTRLHRILRNLCYHAPTRDWVVKCLLSILEKANTGAECPA--QQTMADPSTSG-ANTPTPAK 5410 HSP 4 Score: 119.013 bits (297), Expect = 2.332e-25 Identity = 91/164 (55.49%), Postives = 113/164 (68.90%), Query Frame = 0
Query: 2205 PVIRRNXXXXXXXXXXXXXXXIGRPDSLNNKDDXXXXXXXXXXXXXNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNF------EHDRSGKDKGGLLDRFTKETVVLNAPSK 2362
PVI+R+SLLTDKLLRLLSLIS+G+PD L D+ E G + + D ++LAV+VLTSK+CSEEGLEDVTALLLNLS+G TR+ IL LLL GA++LGNVV A+V DL EL +K++ E + GK KG + DRFTKE VVL AP+K
Sbjct: 5933 PVIKRSSLLTDKLLRLLSLISLGQPDVLKRLDESARQEENTEGGIL--VDKAVKEDQIQLAVEVLTSKACSEEGLEDVTALLLNLSYGGTQTRESILHLLLAGARQLGNVVSAHVSDLLTELAELKASGGLASLPKEEEEEGKHKGVMADRFTKEAVVLVAPTK 6418 HSP 5 Score: 105.145 bits (261), Expect = 3.902e-21 Identity = 67/163 (41.10%), Postives = 98/163 (60.12%), Query Frame = 0
Query: 1451 PSHPLLMGRQNLETGTGNARNSASTRSLPRTRGNSLRPYFQLNSR-AQNNPSTPAILQNFLGGSNPQELITF----------GFRRSGAPLMMDV-VGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE 1601
PSHPLLMGR G+AR RSL R RG Y QLN R P TPAILQ+ LG ++ ++ + R + L+M+ G ++LD++++++P L+ G +ALS++P+AL+RW EESRV+DG+S+HDC+T KPE+++VIE
Sbjct: 3149 PSHPLLMGRTEQVVPAGSARGQG--RSLTRQRGFR---YIQLNPRNGGGGPGTPAILQSLLGHNSGRDFLQLTGNHPGLNVGAMRDATRVLVMESGSGFAILDSLEDEIPGLEGLGQGG--GSALSTVPNALVRWTEESRVLDGDSLHDCMTVCKPELLDVIE 3616
BLAST of EMLSAG00000004991 vs. C. finmarchicus
Match: gi|592944263|gb|GAXK01014290.1| (TSA: Calanus finmarchicus comp8631_c3_seq2 transcribed RNA sequence) HSP 1 Score: 847.04 bits (2187), Expect = 0.000e+0 Identity = 413/505 (81.78%), Postives = 448/505 (88.71%), Query Frame = 0
Query: 2453 LGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMEXXXXXXXXXXXXXXPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTXXXXXXXXXXXXXXAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
L +CLK+LA+TPDHHAVLVLQ +VEAFFLVHAA +EKKK QKETR+EQLAHI+E+ E E + S +SPDTKKFLAFAETHRTVLNQILRQST HLADGPFSVLVDHTRVLDFDIKRRYFRTELER+DE +RREDLAVHVRR+ VFEESFREL RRS EE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNP YALFK+SPGD+VTY I + S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHIL K VK+ DMESEDY FYKGL+FL+ +KVSDLGYDLTFSTEIQEFGVTE+RDLIP+GRN+ VT++ K DYIRLVCQMKMTGAIRKQL+AFLEGFY+IIPRRLI+IFNEQELELLLSGLP I+IDDLK+N+EYHKY SLQI+WFWRALRSFDQTD+AKF+QFVTGSSKVPLQGF LEGMNG QKFQIHRDDRSTDRLP+AHTCFNQLDLPAYETYDKLRTYLLKAI ECSE F
Sbjct: 7160 LSNCLKELADTPDHHAVLVLQPTVEAFFLVHAAVISSEEKKKPNQKETRKEQLAHIEEK-EGQLESEQAPQTVVGSEEDISPDTKKFLAFAETHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERLDEGIRREDLAVHVRRDAVFEESFRELHRRSAEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPNYALFKSSPGDRVTYTINDFSHINSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKLVKHQDMESEDYEFYKGLDFLLENKVSDLGYDLTFSTEIQEFGVTEVRDLIPDGRNVIVTDQNKADYIRLVCQMKMTGAIRKQLAAFLEGFYDIIPRRLIAIFNEQELELLLSGLPDINIDDLKANSEYHKYSVTSLQIVWFWRALRSFDQTDRAKFLQFVTGSSKVPLQGFGALEGMNGAQKFQIHRDDRSTDRLPAAHTCFNQLDLPAYETYDKLRTYLLKAIQECSEGFGF 8671 HSP 2 Score: 811.601 bits (2095), Expect = 0.000e+0 Identity = 504/1033 (48.79%), Postives = 650/1033 (62.92%), Query Frame = 0
Query: 454 EQEDLMRAIAMSL--DVDVVSFQVSSNTEGNNXXXXXXXXXXXXXXXXXXXFGLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIXXXXXXXXXXXXSQSPIEEDVFRTTESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCSGSVG--TPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK--SKGSRDAVVK------------------------------XXXXXXXXXXXMELGEEEKKVSDPNEEDVITTIPT-----------AEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTS-KEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV------------------KTPDICE-SSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKDMKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSAR---KSKNDVPDSSAEDXXXXXXXXXXXXVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERDGSD----NFTIIP--QVGSN------VNSDQDHHQDGSRFAGH-GCQDDVDRAXESSQ 1403
EQ+DLMRAIAMSL +V V + + + ++ +QQ +EEE + L + V+D FT AL GCL L+DTLPETV+R DLL+A+F RNGKEFKEK+L L+ EVK +++ LL EE V ES +AAVRIHLF LLFE+C LC ++VE S + +M L+ V+ + L+ +VG TPKW+TP+LLFIDLYEKV+L M RR ++ S+CS WKW+D+ +G+W Y P +NKTI+DAFW+GE SV+ TTGRRKY IQFG M+QANEETGNRRP+MI++K K + AV + S + N E + E +D +++ I +I GL +D+ ALL+A VG +++PV+ DALNA+LRLCLRLT+ F QA FA LGG+KLLL L Q+S FSG +SLA+LLVRHVLED TL+HTMEK+IR+S S + KE++Y+LR LAPAACR P F SVA+DILR + S L+KRGE ++D RLLVKSL KA ++ APPL +VS+SVI DLL+FLV K+ DI E ++ A+++ S T P V+R + + +PG + + K+ L KSAVCRLLAE+VKSY GCA+LIT+HVY++GI + +KED +ALAF+LDELLTS DK+ LVK L+AALASCNH PEAQ++LV EVK AL R+L PE +KH+K+Q+LT LISTMIE+CP+S+ T QP FK Q MNNIVK M+KRGLITDLARV HALDLSSP++A T+N+ALKPLETLSRIVNQPT L+ + K KND ++ A D + N TNTTNSEATRAQ DE D EATEHD+STTAES+DPNSESQL TVE+ + ED+DEMM+QLLE+D D +I + G N +N Q Q+ S G G ++D D SQ
Sbjct: 104 EQDDLMRAIAMSLGENVMVSTDGGEAGAQQGETKEGEQQKDEEEEMSSDEQEALKQQVIDTFTDTALTGCLTLLDTLPETVYRVTDLLMAIFNRNGKEFKEKLLTQLMGEVKKSVEDLLAVAHSG-------EEAVKDAPESARAAVRIHLFTLLFEDCSRLCVQLVEGSGAIELMVQLIGVAQEALQGAP-AVGRETPKWITPMLLFIDLYEKVVLAMKRRDAMASICSHSWKWFDVGAGKWQGYQPGNNKTINDAFWAGEPSVKFTTGRRKYNIQFGAMMQANEETGNRRPLMITLKRDEKEADTAVERRGRWKKEDSAAAPGEKKEEEDKDESENTTTESTAAPVEANTAEPEKMETSQTDAEKQETIDSIAAKSTPPPHTTYKCSGLSSDQSQALLRAAVGLIAVPVEPDALNAILRLCLRLTRTFSQATLFAELGGIKLLLGLKQASSFSGFSSLASLLVRHVLEDDLTLKHTMEKIIRSSAATSTAATTKELHYLLRSLAPAACRQPDTFTSVARDILRVDISLLSKRGEPEDDHRLLVKSLLGKA-SAPAPPLKDVSKSVISDLLDFLVQSDPDEPLESDQVGDIIGKSDDIAELPTSVASILNSGTVAVQGSSTRGPAVVRQISSELIPG--EKKEEVVKDGKETKEVEDARKKRHLLPKSAVCRLLAEMVKSYAGCARLITEHVYQAGITDLVKEDCTALAFILDELLTSTKDKESATLVKMLVAALASCNHAPEAQTSLVTEVKNALTRALSLPECSMKHTKVQSLTGLISTMIENCPASL-ATNQPPFKSG-QVNMNNIVKCMVKRGLITDLARVSHALDLSSPSMAATVNAALKPLETLSRIVNQPTGLLPPTQNKTKPKND--EARANDINNGN-----------------------TNTTNSEATRAQNDEIAGIDNEATEHDISTTAESMDPNSESQLHTVEEGDGEDFDEMMEQLLEQDRGDAALLEVAVIARGETGQNMETDDTINDSQMMSQEESFVEGEGGAEEDSDTDSSHSQ 3088 HSP 3 Score: 327.791 bits (839), Expect = 1.198e-88 Identity = 197/331 (59.52%), Postives = 245/331 (74.02%), Query Frame = 0
Query: 1877 PIPSTSGTS--QNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS---NVPEVNPEFLAALPPNIQEEVLAXXX-XXXXXXXXXVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMXXXXXXXXXXXQSLRREXEQRNRNFMHERFFNN------GGNSLSSILRGSVSRLGNQYIIGNLVG---REAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVK 2192
P+P +SGTS ++PD+SSILGD++IPEGVDPSFLAALPE+MR EVI++QRRL RQ+ Q G+ + EVNPEFLAALPPNIQEEVLAQQR +Q++ +Q +P APVDPGEF QTLP SLRQS+LADME+SQ++ALPA+LAAEAQ+LRR+ EQRNR MHERFFN+ G +LSSILR +V+RLG+QY I G R S R ++ AN+++F+GRQLLD+EGLSCLLILLF+DD+K+NTTRLHRILRNLCYHAPTRDWVV+CL+SILE+AN + A Q + P+ S ++T TP K
Sbjct: 4739 PLPGSSGTSTIRDPDFSSILGDLDIPEGVDPSFLAALPEDMRAEVIEEQRRLVRARQQPPAQPPPGTTAQGMQEVNPEFLAALPPNIQEEVLAQQRLEQQRQNAAQADPTAPVDPGEFLQTLPPSLRQSLLADMEESQISALPADLAAEAQNLRRDYEQRNRQMMHERFFNHVNHTGPSGPTLSSILRNTVNRLGSQYAIHTGAGGPGRSNLWRSIGR-----GGPGHQQSAAALAAANNVKFRGRQLLDHEGLSCLLILLFIDDAKLNTTRLHRILRNLCYHAPTRDWVVKCLLSILEKANTGAECPA--QQTMADPSTSG-ANTPTPAK 5707 HSP 4 Score: 119.783 bits (299), Expect = 1.625e-25 Identity = 91/164 (55.49%), Postives = 113/164 (68.90%), Query Frame = 0
Query: 2205 PVIRRNXXXXXXXXXXXXXXXIGRPDSLNNKDDXXXXXXXXXXXXXNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNF------EHDRSGKDKGGLLDRFTKETVVLNAPSK 2362
PVI+R+SLLTDKLLRLLSLIS+G+PD L D+ E G + + D ++LAV+VLTSK+CSEEGLEDVTALLLNLS+G TR+ IL LLL GA++LGNVV A+V DL EL +K++ E + GK KG + DRFTKE VVL AP+K
Sbjct: 6230 PVIKRSSLLTDKLLRLLSLISLGQPDVLKRLDESARQEENTEGGIL--VDKAVKEDQIQLAVEVLTSKACSEEGLEDVTALLLNLSYGGTQTRESILHLLLAGARQLGNVVSAHVSDLLTELAELKASGGLASLPKEEEEEGKHKGVMADRFTKEAVVLVAPTK 6715 HSP 5 Score: 105.531 bits (262), Expect = 3.211e-21 Identity = 67/163 (41.10%), Postives = 98/163 (60.12%), Query Frame = 0
Query: 1451 PSHPLLMGRQNLETGTGNARNSASTRSLPRTRGNSLRPYFQLNSR-AQNNPSTPAILQNFLGGSNPQELITF----------GFRRSGAPLMMDV-VGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE 1601
PSHPLLMGR G+AR RSL R RG Y QLN R P TPAILQ+ LG ++ ++ + R + L+M+ G ++LD++++++P L+ G +ALS++P+AL+RW EESRV+DG+S+HDC+T KPE+++VIE
Sbjct: 3446 PSHPLLMGRTEQVVPAGSARGQG--RSLTRQRGFR---YIQLNPRNGGGGPGTPAILQSLLGHNSGRDFLQLTGNHPGLNVGAMRDATRVLVMESGSGFAILDSLEDEIPGLEGLGQGG--GSALSTVPNALVRWTEESRVLDGDSLHDCMTVCKPELLDVIE 3913
BLAST of EMLSAG00000004991 vs. L. salmonis peptides
Match: EMLSAP00000004991 (pep:novel supercontig:LSalAtl2s:LSalAtl2s260:299952:311628:1 gene:EMLSAG00000004991 transcript:EMLSAT00000004991 description:"maker-LSalAtl2s260-augustus-gene-3.39") HSP 1 Score: 6483.28 bits (16819), Expect = 0.000e+0 Identity = 3165/3165 (100.00%), Postives = 3165/3165 (100.00%), Query Frame = 0
Query: 1 MDIDSSSSTSATTIEKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKGEDDKNSGGGAMPSRVASRFANSFSNSPPFQMSLPDFLGDSFLSPVINNEAGGGXAAQGGEQPQXVQQPEQPPTLQPEQQQQSXSQNAPPVITPXATAENNDDVEPAPGFSFYDLQILRDMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSPIEEDVFRTTESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCSGSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIKSKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPNEEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLVKTPDICESSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKDMKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSARKSKNDVPDSSAEDSTSNNPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERDGSDNFTIIPQVGSNVNSDQDHHQDGSRFAGHGCQDDVDRAXESSQXDAAIEEETEDLIEEEEDDDGEEEDDEVNGGSGVSTDGGANSANHVTPSHPLLMGRQNLETGTGNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFRRSGAPLMMDVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIENTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAATTALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVPPLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRNMDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQPRESEEAIGGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVKSASSQQQQQXKYPVIRRNSLLTDKLLRLLSLISIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNFEHDRSGKDKGGLLDRFTKETVVLNAPSKQXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEENKMDVDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEFEHLEVAFPWEITRIRSDLVPRPLESQDEHKGGATPSRKPIPHWSGVHRIVSTVLDEMGKASALAQGLNHVVTSSEKLRHSEEQVVYILVLHSRVAGLLKVGRKNLFLLDKSGKQNEKFPLCILDFYVAESLQRSGLGNKLFQAMLKDQGDIHPRFLAIDRPSPKLISFFEKHYQLIQNIPQVNHYHIFQGFFTDXPQXEEGNAQAKRA 3165
MDIDSSSSTSATTIEKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKGEDDKNSGGGAMPSRVASRFANSFSNSPPFQMSLPDFLGDSFLSPVINNEAGGGXAAQGGEQPQXVQQPEQPPTLQPEQQQQSXSQNAPPVITPXATAENNDDVEPAPGFSFYDLQILRDMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSPIEEDVFRTTESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCSGSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIKSKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPNEEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLVKTPDICESSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKDMKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSARKSKNDVPDSSAEDSTSNNPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERDGSDNFTIIPQVGSNVNSDQDHHQDGSRFAGHGCQDDVDRAXESSQXDAAIEEETEDLIEEEEDDDGEEEDDEVNGGSGVSTDGGANSANHVTPSHPLLMGRQNLETGTGNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFRRSGAPLMMDVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIENTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAATTALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVPPLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRNMDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQPRESEEAIGGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVKSASSQQQQQXKYPVIRRNSLLTDKLLRLLSLISIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNFEHDRSGKDKGGLLDRFTKETVVLNAPSKQXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEENKMDVDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEFEHLEVAFPWEITRIRSDLVPRPLESQDEHKGGATPSRKPIPHWSGVHRIVSTVLDEMGKASALAQGLNHVVTSSEKLRHSEEQVVYILVLHSRVAGLLKVGRKNLFLLDKSGKQNEKFPLCILDFYVAESLQRSGLGNKLFQAMLKDQGDIHPRFLAIDRPSPKLISFFEKHYQLIQNIPQVNHYHIFQGFFTDXPQXEEGNAQAKRA
Sbjct: 1 MDIDSSSSTSATTIEKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKGEDDKNSGGGAMPSRVASRFANSFSNSPPFQMSLPDFLGDSFLSPVINNEAGGGXAAQGGEQPQXVQQPEQPPTLQPEQQQQSXSQNAPPVITPXATAENNDDVEPAPGFSFYDLQILRDMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSPIEEDVFRTTESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCSGSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIKSKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPNEEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLVKTPDICESSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKDMKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSARKSKNDVPDSSAEDSTSNNPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERDGSDNFTIIPQVGSNVNSDQDHHQDGSRFAGHGCQDDVDRAXESSQXDAAIEEETEDLIEEEEDDDGEEEDDEVNGGSGVSTDGGANSANHVTPSHPLLMGRQNLETGTGNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFRRSGAPLMMDVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIENTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAATTALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVPPLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRNMDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQPRESEEAIGGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVKSASSQQQQQXKYPVIRRNSLLTDKLLRLLSLISIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNFEHDRSGKDKGGLLDRFTKETVVLNAPSKQXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEENKMDVDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEFEHLEVAFPWEITRIRSDLVPRPLESQDEHKGGATPSRKPIPHWSGVHRIVSTVLDEMGKASALAQGLNHVVTSSEKLRHSEEQVVYILVLHSRVAGLLKVGRKNLFLLDKSGKQNEKFPLCILDFYVAESLQRSGLGNKLFQAMLKDQGDIHPRFLAIDRPSPKLISFFEKHYQLIQNIPQVNHYHIFQGFFTDXPQXEEGNAQAKRA 3165
BLAST of EMLSAG00000004991 vs. L. salmonis peptides
Match: EMLSAP00000011039 (pep:novel supercontig:LSalAtl2s:LSalAtl2s744:109377:113126:-1 gene:EMLSAG00000011039 transcript:EMLSAT00000011039 description:"maker-LSalAtl2s744-snap-gene-1.41") HSP 1 Score: 303.908 bits (777), Expect = 1.690e-85 Identity = 153/413 (37.05%), Postives = 234/413 (56.66%), Query Frame = 0
Query: 2562 ADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVR----RENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGL--------------------LREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHE 2950
A G + V V + R FR +L + + L H++ R+ +FE+SF +L R E + R YI+F GEEG D GG LREW+ ++S E+ NPMY LF+ + + + I +S NP+HL YFKF+GR IA A+Y K + FT FYK +L K + D+ES D +Y L ++ ++ + + G ++ F + + G + +L P G + VTEE K +YI +VC+ +M+ I +Q +FLEGF ++P + F+E+ELEL+L G+ ID+DD + ++ Y Y +NS QI WFW+ +RS D +++ +QFV G+ +VP+ GFA L G NG Q+F + + + + LP +HTCFN+LDLP Y++YD+L L AI E
Sbjct: 466 AKGAYGVPVQYERS---------FRWKLSQFRYLCQSNSLPSHIKLSVTRQTLFEDSFHQLMRLPAFELRRRLYIIFRGEEGLDYGGXARXVDMLLLLPCSYVNLIYPLREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLHYFKFIGRFIAMALYHGKFIYSGFTMPFYKRMLNKKLIMKDIESIDPEYYNSLMWIKDNNIEECGLEMYFCVDFEVLGEIKPHELKPGGSELKVTEENKEEYIEIVCEWRMSRGIEEQTKSFLEGFNEVVPLEWLQYFDERELELMLCGMQVIDVDDWQRHSIYRHYTKNSKQIQWFWQFVRSMDHEKRSRLLQFVCGTCRVPVGGFAELMGSNGAQRFCVEKVGKDS-WLPRSHTCFNRLDLPPYKSYDQLVEKLNFAIEE 868
BLAST of EMLSAG00000004991 vs. L. salmonis peptides
Match: EMLSAP00000007514 (pep:novel supercontig:LSalAtl2s:LSalAtl2s431:6204:41118:1 gene:EMLSAG00000007514 transcript:EMLSAT00000007514 description:"maker-LSalAtl2s431-snap-gene-0.13") HSP 1 Score: 292.352 bits (747), Expect = 4.788e-82 Identity = 156/381 (40.94%), Postives = 224/381 (58.79%), Query Frame = 0
Query: 2579 DIKRRYFRTELERMDERLRR-----EDLAVHVRRENVFEESFRELXRRSPEEX----KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQT-NPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYH-KYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAI 2948
D KR+Y E + +L++ + +RR + E+SF+++ + P K + +I F+GE G D GG+ REW+ ++S+E+FNP Y LF+ S D T I S N +HL YFKF+GR A KLL+ +F R FYK +LGK ++ +DMES D + L ++ + +L +LTF + + FG T R+L P G+NI VT E K +YI V Q + I+KQ+ FL GF ++P I IF+E ELELL+ G+ +ID+ D K NT Y Y N + I W+WR + SF+ +++ +QFVTG+S+VP+ GF L G NG Q F I R + P AHTCFN+LDLP YE+Y +LR L+KAI
Sbjct: 370 DYKRKY-----EFLKSQLKKPSNVPNKFEIKIRRRYILEDSFQKIFMQFPSHKVDVLKTKLWIEFDGEIGLDYGGVAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGVCNEDHLSYFKFIGRXAGMAXXXGKLLDGFFIRPFYKMMLGKGMELSDMESVDTEXFNSLMWIRENDPQEL--ELTFQVDEENFGTTVTRELKPGGKNIPVTNENKDEYIHQVIQWRFVSRIQKQMDNFLAGFNELVPLSFIKIFDEGELELLMCGIGSIDVKDWKQNTVYKGDYHPNHIVIQWYWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLFTIERWGNPSS-YPRAHTCFNRLDLPPYESYQQLRDRLIKAI 742
BLAST of EMLSAG00000004991 vs. L. salmonis peptides
Match: EMLSAP00000006171 (pep:novel supercontig:LSalAtl2s:LSalAtl2s336:651240:657086:1 gene:EMLSAG00000006171 transcript:EMLSAT00000006171 description:"maker-LSalAtl2s336-augustus-gene-6.36") HSP 1 Score: 281.182 bits (718), Expect = 1.029e-77 Identity = 136/358 (37.99%), Postives = 217/358 (60.61%), Query Frame = 0
Query: 2603 VHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLE---GMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHE-CSEVME 2956
+ V R+ VFE+S+R++ + + + + + F EEG D GG+ REW ++S E+ NP Y LF+ S D T I S NP+HL YF FVGR+I A++ ++ FT FYK +L KP+ D+E+ D +K L++++++ +S + D TFS E + FG+T++ +L NG++I V E K++Y++L Q + I +Q + +GF +IP+ L+ F+E+ELEL++ G+ TIDI D K++T + + + WFW + S+ +A+ +QFVTGSS+VPLQGF L+ G G + F +H + D LP A TCFN+LDLPAY +++ + L +A+ E C +E
Sbjct: 576 LEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGI-IDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
BLAST of EMLSAG00000004991 vs. L. salmonis peptides
Match: EMLSAP00000003493 (pep:novel supercontig:LSalAtl2s:LSalAtl2s190:418567:426512:1 gene:EMLSAG00000003493 transcript:EMLSAT00000003493 description:"maker-LSalAtl2s190-snap-gene-4.16") HSP 1 Score: 162.54 bits (410), Expect = 4.882e-40 Identity = 115/363 (31.68%), Postives = 184/363 (50.69%), Query Frame = 0
Query: 2603 VHVRRENVFEESFRELXRRSPEEXKNRFYIVFEG-----EEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKV-----TYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGK---PVKYTDMESEDYAFYKGLEFLVNHK--VSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPT-IDIDDLKSNTEYH-KYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAI 2948
V +RR+ ++E++ +L + K + + F+ E G D GGL RE+ + + F+P F+ + ++ + IYE + L ++ F+GR++ KA+Y+N L+E F +L K V ++S D Y+ L +L N+ V DLG D T E E G T + +L NG +I VT + +YI L+ K+ IR Q +AF +G ++I + +FN +EL+L++SG T ID+ DL+++T Y Y + ++ FW + S +T K ++FVT S+ PL GF L+ F +H RLP+A TC N L LP +E L+ LL AI
Sbjct: 658 VTIRRKFIYEDALEKLSPEAEPNMKLKMRVEFKNIAGLREAGIDGGGLFREFLGELVKTSFDPNRGFFRLTKDQRLYPNPSVHKIYE------DFLPHYFFIGRMLGKALYENMLVEFPLASFFLHKLLEKSSVKVDIDHLQSLDPELYRNLLYLKNYDGCVQDLGLDFTIVNE--EVGTTSVEELKRNGSSIPVTNVNRTEYIHLIANYKLNKQIRLQSNAFRQGLSDVINLDWLKMFNYKELQLVISGAETEIDVSDLRNHTNYTGGYAVDHPTVVIFWNVVESMTETQKRHLLKFVTSCSRPPLLGFKELD-----PPFCVHNAGAEPHRLPTASTCMNLLKLPNFEEEKILKDKLLYAI 1007
BLAST of EMLSAG00000004991 vs. L. salmonis peptides
Match: EMLSAP00000008825 (pep:novel supercontig:LSalAtl2s:LSalAtl2s54:414186:494517:-1 gene:EMLSAG00000008825 transcript:EMLSAT00000008825 description:"maker-LSalAtl2s54-augustus-gene-5.10") HSP 1 Score: 161.384 bits (407), Expect = 1.918e-39 Identity = 118/376 (31.38%), Postives = 187/376 (49.73%), Query Frame = 0
Query: 2603 VHVRRENVFEESFRELXRRSPEEXKNRFYIVF---EG--EEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYES--SQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKP----VKYTD-MESEDYAFYKGLEFL--VNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSG--LPTIDIDDLKSNTEYH-KYQRNSLQIIWFWRAL-RSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTD-------------------RLPSAHTCFNQLDLPAYETYDKLR 2941
+ V R + E+ +R L + S K + F +G E G D G+ +E+ + +F+P LF ++ ++ +Y S S N+L F+FVG++I KA+Y+ ++E F F IL K Y D + S D YK L ++ + V+DL DLTFS + G +LI GR++ V + K+ YI V Q + I++Q++AF GF I+P +++F+ E+ L+SG LP +D+ DL+ +T Y+ + + I+W W L R FD +++ F++FVT SK PL GF LE ++ ++ D+ D RLP+A TCFN L LP Y+ LR
Sbjct: 615 ITVHRSRIVEDGYRHLGKLSSRSLKGVIRVKFINTQGLDEAGIDQDGVFKEFLEETIKRVFDPGLNLFCSTSDNR----LYPSPTSHLTENYLQLFEFVGKMIGKAVYEGIVVEIPFAPFFISQILSKDHSAYYSYLDELPSMDPELYKNLTYIKYFDGNVTDL--DLTFSYDQDVLGRVVTHELIEGGRSVLVNDCNKISYIHHVAQFVLHSQIKEQVAAFTRGFRAILPIDWLNLFSPPEVLRLISGDNLP-LDLKDLRRHTHYYGGFHDSHKVIVWLWEILDRDFDDKERSSFLKFVTSCSKPPLLGFENLEPAFSIRCVEVAEDEDDGDTVGSVLRGFLALKKRDPVNRLPTASTCFNLLKLPNYQKKATLR 983
BLAST of EMLSAG00000004991 vs. L. salmonis peptides
Match: EMLSAP00000007808 (pep:novel supercontig:LSalAtl2s:LSalAtl2s458:200727:208701:-1 gene:EMLSAG00000007808 transcript:EMLSAT00000007808 description:"maker-LSalAtl2s458-snap-gene-2.45") HSP 1 Score: 139.043 bits (349), Expect = 2.119e-33 Identity = 114/426 (26.76%), Postives = 190/426 (44.60%), Query Frame = 0
Query: 2542 FAETHRTV--LNQILRQSTAHLAD-------GPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXR---RSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYE-SSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKG-LEFLVNHKVSDLGYDLTFSTEI--QEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQII-WFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHE 2950
F H T+ + I + T H G F + + FD K F + E M E + ++V R + S + L + P I F GE+G + GGL REWY ++ +E+ D ++ E + NP Y F F+ SF + G + + + FY ++++V +KV DL DL F+ EI ++ + E ++L P+G+ I VT K +Y+ L+ + +++ I+ L+ F G + + ++ F+E E++LL SG TID +DLK ++H Q S +I+ WFW L + K F++F+TGSS++P GF L F I R +LPS+H C N L++ + +++ + + AI E
Sbjct: 291 FLLNHSTIFSITDIFKNKTLHFKLNERLFLLGSFVNAANKKCSMTFDEKLVEFFSNFEYMGELIFLMQSIINVDRHCLLYSSIKSLEQCWWIGP------LKISFSGEQGIEYGGLRREWYTLLWKEL------------SDTKIFITNERGTGANPMFRLYLPFS-----------------FSSSFLYQLKGINIGXRHFKVDYPEFYTSRIDYIVKNKVEDL--DLYFTDEIYDEKGHLIESKNLKPSGKKIKVTNFNKFEYLHLLAKYRLSDRIKGPLTKFKTGLHYYVSETYLANFDESEMDLLFSGFSTIDFNDLK---KHHNCQNVSHKIMEWFWLVLEHLSEEQKLLFLKFITGSSRLPPNGFKDL-----CPVFTI-RQLNEEGKLPSSHVCANVLNIHPHSSFEAFESSFITAITE 670
BLAST of EMLSAG00000004991 vs. L. salmonis peptides
Match: EMLSAP00000000248 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1036:68871:78712:-1 gene:EMLSAG00000000248 transcript:EMLSAT00000000248 description:"maker-LSalAtl2s1036-augustus-gene-0.34") HSP 1 Score: 105.916 bits (263), Expect = 1.597e-22 Identity = 80/293 (27.30%), Postives = 132/293 (45.05%), Query Frame = 0
Query: 2669 PGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLV----NHKVSDLGY---DLTFSTEIQ-EFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQII-----WFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAI 2948
PG + Y + T L+ F+ VGR++ + N+L + R K+ILG+ +++ D+ D Y+ L LV N + S + DL FS ++ E G + +LIPNGR + Y+R +M K LS G Y+++P + ++ LLL+G+ I++ L S T ++ S + W W+ + +K + F TGS +P A +G + I S LPSA+TC ++L +P Y + L++ LL AI
Sbjct: 2707 PGKRGFYSPVQGKATQ-ERLNAFRNVGRLMGLCLLQNELCPLFLNRHVIKYILGRTIRFHDLAFFDPVIYESLRQLVVDAENKETSAQLFSALDLNFSIDLSPEEGGGSV-ELIPNGREVEXXANNVYCYVRRYSYYRMIKCQEKALSNIKIGLYDVLPAGALDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGESQDRLVNFKRWLWQMVEKMSPLEKQDLVYFWTGSPALP----ASEDGFQPMPSITIRPAGDS--HLPSANTCISRLYVPLYSSKAILKSKLLLAI 2991
BLAST of EMLSAG00000004991 vs. L. salmonis peptides
Match: EMLSAP00000002245 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1426:7958:26313:1 gene:EMLSAG00000002245 transcript:EMLSAT00000002245 description:"maker-LSalAtl2s1426-augustus-gene-0.12") HSP 1 Score: 97.4413 bits (241), Expect = 6.656e-20 Identity = 94/383 (24.54%), Postives = 160/383 (41.78%), Query Frame = 0
Query: 2596 LRREDLAVHVRR------ENVFEESFRELXRRSPEEXK---NRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSP------GDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEI---QEFGVTEIRDLIPNGRNITVTEETKLDYI------RLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEV 2954
LR V VRR ++F + ++ P+E + + + GE DAGG+ + + +E+ + L +P G + S + + + +F F+G + A+ K + +K IL + D+E D ++ + L ++ D D T+ F E+ L P I ++ E K +Y+ R M A+RK LSA + P ++ + Q +E L+ G + + LK Y ++ I+W W L F+ K F+ FV+G S++P F + Q+FQI R +R + LP+A TCF QL LP Y + + +R L+ AI C +
Sbjct: 2968 LRNYGPQVTVRRLFPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRHFWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVATSFNPDNEEDSLLPTDYFMGISFSGREV-PLYPGSEKIRLSYENKDEYVKAALNQRFKEMMPQVLAVRKGLSA-------LAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAF------DVSQRFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIKHCRTI 3336
BLAST of EMLSAG00000004991 vs. L. salmonis peptides
Match: EMLSAP00000009470 (pep:novel supercontig:LSalAtl2s:LSalAtl2s60:50964:63500:-1 gene:EMLSAG00000009470 transcript:EMLSAT00000009470 description:"maker-LSalAtl2s60-augustus-gene-0.20") HSP 1 Score: 93.5893 bits (231), Expect = 7.546e-19 Identity = 105/432 (24.31%), Postives = 180/432 (41.67%), Query Frame = 0
Query: 2590 ERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFK-----------------TSPGDKVTYM--IYESSQTNPNHL-------------DYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGK--PVKYTDMESEDYAF---YKGLEFLVNHK---------------------------VSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDID--DLKSNTEY----HKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLE-GMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHE 2950
ER+ L R+ + R +N+ ++S + + +P ++ I +E E G G L E+Y ++S+E+ L+K DK + + I+ S PN + + F F+G+ IAKA+ DN++++ F++ FY+ +L + + +D+ + D LE + K + DLG D T G I DL G++I V + Y+ LV + I Q+ A +GF ++ P + +F +ELE + G + + D K+ E H + S + + L S++ ++ +F+QFVTG ++P+ GF L + V+K + D D LPS TC N L LP Y + +R L A E
Sbjct: 1315 ERVTPDLDRKKRVIS-RDDNLLKQSEQVMNELAP--SRSLLEIQYENEVGTGLGPTL-EFYTLVSKELQKADLGLWKGETVKISNEDVMDSNEDNERNDKSSCIEYIHSSVGLYPNPMARNVKSSQRSKIKNKFTFLGKFIAKAVLDNRMIDMPFSQPFYQWLLREESTLGISDLRNVDPTIASTISSLEGIARKKRKLEQDPQISPDEKLEQIKSLTMDGCPIEDLGLDFTLP------GYPNI-DLRKGGKDIPVNIDNLHQYVGLVSHWLLIEGISFQMEALRDGFESVFPISTLQMFYPEELEQIFCGTGQGNFNKWDYKTLVESTKPDHGFNSESPALKYLLEVLESYNIDEQRQFLQFVTGCPRLPVGGFKALNPPLTIVKKSFDNADADPDDYLPSVMTCVNYLKLPDYSSKKIMREKLRIAAME 1735
BLAST of EMLSAG00000004991 vs. SwissProt
Match: gi|73915353|sp|Q7Z6Z7.3|HUWE1_HUMAN (RecName: Full=E3 ubiquitin-protein ligase HUWE1; AltName: Full=ARF-binding protein 1; Short=ARF-BP1; AltName: Full=HECT, UBA and WWE domain-containing protein 1; AltName: Full=HECT-type E3 ubiquitin transferase HUWE1; AltName: Full=Homologous to E6AP carboxyl terminus homologous protein 9; Short=HectH9; AltName: Full=Large structure of UREB1; Short=LASU1; AltName: Full=Mcl-1 ubiquitin ligase E3; Short=Mule; AltName: Full=Upstream regulatory element-binding protein 1; Short=URE-B1; Short=URE-binding protein 1) HSP 1 Score: 838.951 bits (2166), Expect = 0.000e+0 Identity = 401/568 (70.60%), Postives = 467/568 (82.22%), Query Frame = 0
Query: 2412 EENKMDVDENS----------SDSTSEAKANSQWK---IESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQL----------ADNKEME---SSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
EE+ MDVD+ S SD T + + + + + LSEQLSLD LW+ LG+CLK+L E+ D HAVLVLQ +VEAFFLVHA T+ + K V ++TR+ QLAHI+++ A ++ S +S S+SL PDT+KFL FAETHRTVLNQILRQST HLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE LR+ED+AVHVRR++VFE+S+REL R+SPEE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 3806 EESPMDVDQPSPSAQDTQSIASDGTPQGEKEKEERPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHA--TERESKPPV--RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 4369 HSP 2 Score: 560.451 bits (1443), Expect = 6.250e-159 Identity = 409/1080 (37.87%), Postives = 586/1080 (54.26%), Query Frame = 0
Query: 397 DMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTE--GNNEEDSQQQSGKEEEENEGGSFG-------LSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSP-IEEDVFRTT---ESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCS--GSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVC---SRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK-----SKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPN--------------EEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIR---TSTLNSQTT--------SKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETD---EDTRL-------LVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV--KTPDICESSN--NATVVESATELKNNKGGGAPNVIRNMX--TGDL---PGVLXXPPVTITTAQPCDTSA-------------SKDMKK-------------------PFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELL---TSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSARKSKNDVPDSSA----EDSTSN--NPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERD-GSDNFTII 1366
DMGF+RE +EA+ T M +ATEYLLT +R +S E++ +MRAIAMSL D+ Q + + E +E+ ++++ +++EE E L + + FT LPGC +L+D LP+TV+R CDL++ RNG ++++ IL+ ++ +V A DVL+ L L S + + E + + +++ A RI L LLFEE KL CA +VE S ++ ++ LL V L+ V TPKW+TP+LL ID YEK + RRA +T S W+W+D SGRWC Y+ ++N TID A+ SGETSVR T GRR+YT+QF TM+Q NEETGNRRP+M+++ +K S+++ + + + M++ +E K +D EE I I +GL D + L++ACV + +PVD D L+A LRLCLRLT++ + A+ FA L +++L L QSS F+G T L TLL+RH++EDP TLRHTMEKV+R TS S T+ S+EINYILRVL PAACR+P IF VA +R P+P +D E+ R+ LVK+ P K S P + + + VI D+LN L P+ + S+ + + +L + G R++ + D G V +TSA S+D KK P + S + RLLAELV+SY G A LI ++ Y G E IKED S LAF+LD LL + DKD L + +A+LA+ +AQ ALV EVK AL R+L ES KH+++QA+ C+IST++ESCPS+ A + MNNI+++ LK+GL+ DLARVPH+LDLSSPN+A T+N+ALKPLETLSRIVNQP++L S S + + A +DS+SN +P G + ++ E + +V T +E A GD D EA E D A + S ++Q + + ++++D+LLERD GS N TII
Sbjct: 1327 DMGFTREHAMEALLNTSTMEQATEYLLTHPPPI------------MGGVVRDLSMS-EEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPLALESTNTEKETSLEETKIGEI-LIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKP--SPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEASTPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPST-SSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGASQDSSSNQQDPGEPGEA----EVQEEDHDV------TQTEV--ADGDIM---DGEA-ETDSVVIAGQPEVLSSQEMQV-----ENELEDLIDELLERDGGSGNSTII 2368 HSP 3 Score: 364.385 bits (934), Expect = 3.514e-99 Identity = 171/283 (60.42%), Postives = 206/283 (72.79%), Query Frame = 0
Query: 12 TTIEKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNL---SIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSK---GSKGEDDKNSGGGA 288
T + K +K P + +IKQIKPLL ASSRLGRALAELF LLVKLCVGSP+RQRR H T AP+ A + ASAL +LL GLSW PPP +P P FRLTFF CSVGFT PMLFD++KYPYHLML KF+ SGG A F+TF WALS GGK+ EGLE ++LPDGTGEFLD+WLML+E+MVNP VLESPH+LP + NF F +++L+ T K AF + +W + PLKVYG RMAES+L+ILCHIL+GE +I E+LSK GS+GE+D G
Sbjct: 1028 TASDSKGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEGSRGEEDTGQEEGG 1310 HSP 4 Score: 292.352 bits (747), Expect = 2.062e-77 Identity = 260/764 (34.03%), Postives = 368/764 (48.17%), Query Frame = 0
Query: 1467 GNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFR-----RSGAPLMM-----DVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE---------NTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAAT-----TALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVP-------PLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRN--MDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQP---------------RESEEAI-GGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGS------NVPEVNPEFLAALPPNIQEEVLAQQRMEQQR-QLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNS-LSSILRGSV--SRL----GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
G R+ + R L G+++ ++ N P+ P ILQ LG S +++ R A L++ ++ S + +D+ D +AT +G LSSIP+AL RW EE +V+D ESMHDCV+ +K I+ +E ++ EEE KI K D N D+ A T + + TE G TP+ P TTPS+T AAT T T SS+ A+ ++ Q+L + +++P P S TT + S + S + + ++SQ MDT++ A T L+ A +S S+T +V + G S ES QP R+S AI G S P+PSTS ++P L + +PEGVDPSFLAALP+++R+EV+ +Q ++ + A N+ V EV+PEFLAALPP IQEEVLAQQR EQQR +L+Q + + P+DP F QTLP+ LR+SVL DMEDS +A +P ++AAEAQ+LRRE E R R MHER F + S LS+ILR SRL G QY + F++ GS + R + + +R +GR LLD+E LSCLL+LLFVD+ K+NT+RLHR+LRNLCYHA TR WV+R L+SIL+R++E++
Sbjct: 2542 GIGRSQRTLRQLTANTGHTIHVHYPGN----RQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAG---TLSSIPTALTRWTEECKVLDAESMHDCVSVVKVSIVNHLEFLRDEELEERREKRRKQLAEEETKITDKGKEDKENRDQS-AQCTASKSNDSTEQNLSDG-------TPMPDSYP---------------------TTPSSTDAATSESKETLGTLQSSQQQPTLPTPPALGEVPQELQSPAGEGGSSTQLLMPVEPEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCT---------------LEEAVGDTSAAGSSEQPRAGSSTPGDAPPAVAEVQGRSDG---SGESAQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDP-----LAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRTAPSTNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSS---------SHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE 3237 HSP 5 Score: 67.781 bits (164), Expect = 1.741e-9 Identity = 48/123 (39.02%), Postives = 65/123 (52.85%), Query Frame = 0
Query: 2257 ISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDL---------------QNELLSIKSTNFEHDRSGKDKGGLLDRF-TKETVVLNAPSKQ 2363
++ + L+L+V+VLTS SCSEEGLED +LL LS G TRD +L+LLL GA+ LG + + L Q E LS E ++ K KG + RF E VV+ A K+
Sbjct: 3576 LTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKR 3698
BLAST of EMLSAG00000004991 vs. SwissProt
Match: gi|341941147|sp|Q7TMY8.5|HUWE1_MOUSE (RecName: Full=E3 ubiquitin-protein ligase HUWE1; AltName: Full=E3Histone; AltName: Full=HECT, UBA and WWE domain-containing protein 1; AltName: Full=HECT-type E3 ubiquitin transferase HUWE1; AltName: Full=Upstream regulatory element-binding protein 1; Short=URE-B1; Short=URE-binding protein 1) HSP 1 Score: 836.254 bits (2159), Expect = 0.000e+0 Identity = 402/569 (70.65%), Postives = 466/569 (81.90%), Query Frame = 0
Query: 2412 EENKMDVDENS-----------SDSTSEAKANSQWKIESL---SEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQL----------ADNKEME---SSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
EE+ MDVD+ S SD T + + + K L SEQLSLD LW+ LG+CLK+L E+ D HAVLVLQ +VEAFFLVHA T+ + K V ++TR+ QLAHI+++ A ++ S +S S+SL PDT+KFL FAETHRTVLNQILRQST HLADGPF+VLVD+ RVLDFD+KR+YFR ELER+DE LR+ED+AVHVRR++VFE+S+REL R+SPEE KNR YIVFEGEEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 3808 EESPMDVDQPSPSAQDTQSIVISDGTPQGEKEKEEKPPELPLLSEQLSLDELWDMLGECLKELEESHDQHAVLVLQPAVEAFFLVHA--TERESKPPV--RDTRESQLAHIKDEPPPLSPAPLTPATPSSLDPFFSREPSSMHISSSLPPDTQKFLRFAETHRTVLNQILRQSTTHLADGPFAVLVDYIRVLDFDVKRKYFRQELERLDEGLRKEDMAVHVRRDHVFEDSYRELHRKSPEEMKNRLYIVFEGEEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 4372 HSP 2 Score: 386.726 bits (992), Expect = 5.617e-106 Identity = 254/646 (39.32%), Postives = 364/646 (56.35%), Query Frame = 0
Query: 397 DMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTE--GNNEEDSQQQSGKEEEENEGGSFG-------LSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSP-IEEDVFRTT---ESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCS--GSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVC---SRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIKSKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPNEEDVITTIPTA----------------------EGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIR---TSTLNSQTT--------SKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETD---EDTRL-------LVKSLPTKAPNSVAPPLGEVSQSVICDLLNFL 980
DMGF+RE +EA+ T M +ATEYLLT +R +S E++ +MRAIAMSL D+ Q + + E +E+ ++++ +++EE E L + + FT LPGC +L+D LP+TV+R CDL++ RNG ++++ IL+ ++ +V A DVL+ L L S + + E + + +++ A RI L LLFEE KL CA +VE S ++ ++ LL V L+ V TPKW+TP+LL ID YEK + RRA +T S W+W+D SGRWC Y+ ++N TID A+ SGETSVR T GRR+YT+QF TM+Q NEETGNRRP+M+++ R + ++ SSN + EE K+ ++E+ IP A +GL D + L++ACV + +PVD D L+A LRLCLRLT++ + A+ FA L +++L L QSS F+G T L TLL+RH++EDP TLRHTMEKV+R TS S T+ S+EINYILRVL PAACR+P IF VA +R P+P +D E+ R+ LVK+ P K P+S+ P + + + VI D+LN L
Sbjct: 1327 DMGFTREHAMEALLNTSTMEQATEYLLTHPPPI------------IGGVVRDLSMS-EEDQMMRAIAMSLGQDIPMDQRAESPEEVACRKEEEERKAREKQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQKEVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLL----RVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGEILIQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRIALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLK-PSSL-PVIPDTIKEVIYDMLNAL 1953 HSP 3 Score: 353.214 bits (905), Expect = 7.571e-96 Identity = 162/258 (62.79%), Postives = 195/258 (75.58%), Query Frame = 0
Query: 24 PCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNL---SIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKG 278
P + +IKQIKPLL ASSRLGRALAELF LLVKLCVGSP+RQRR H T AP+ A + ASAL +LL GLSW PPP +P P FRLTFF CSVGFT PMLFD++KYPYHLML KF+ SGG A F+TF WALS GGK+ EGLE ++LPDGTGEFLD+WLML+E+MVNP VLESPH+LP + +F F +++L+ T K AF + +W + PLKVYG RMAES+L+ILCHIL+GE +I E+LSK +G
Sbjct: 1040 PAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAKLPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKEKEG 1297 HSP 4 Score: 298.516 bits (763), Expect = 2.663e-79 Identity = 260/759 (34.26%), Postives = 371/759 (48.88%), Query Frame = 0
Query: 1467 GNARNSASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFGFR-----RSGAPLMM-----DVVGLSLLDTMDNDMPDLDSATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIE---------NTRDEVRRKKEEERKIIKKLIIDTNNLDEDPAATTTTTTGGGTEGAAGTGSDLIAVSTPLSTVSPVVRAIEGSNNTTLNVSTTNTTTTPSTTAAATTALTSSSSEDTSAPSGRQAISQLAQDLAAAISTRVGCLPIIVP-------PLTSSSTTSTTSLSQSDQQSELQQSHRESHQGEQQFQLNSQSRN--MDTTTSARLTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQP---------------RESEEAI-GGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQ------RRLQNIRQRAQQQNTEGSNVPEVNPEFLAALPPNIQEEVLAQQRMEQQR-QLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNS-LSSILRGSV--SRL----GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
G R+ + R L G+++ ++ N P+ P ILQ LG S +++ R A L++ ++ S + +D+ D +AT +G LSSIP+AL RW EE +V+D ESMHDCV+ +K I+ +E ++ EEE KII K D N D+ A T T T TE G TP+ P TT ++T T+ + T T S+ A ++ ++ Q+L + +++P P S TT + S + S + + ++SQ MDT++ A T + + + +P A+ S T+ ++T G SV DVS ES+QP R+S AI G S P+PSTS ++P L + +PEGVDPSFLAALP+++R+EV+ +Q R + V EV+PEFLAALPP IQEEVLAQQR EQQR +L+Q + + P+DP F QTLP+ LR+SVL DMEDS +A +P ++AAEAQ+LRRE E R R MHER F + S LS+ILR SRL G QY + F++ GS + R + + +R +GR LLD+E LSCLL+LLFVD+ K+NT+RLHR+LRNLCYHA TR WV+R L+SIL+R++E++
Sbjct: 2542 GIGRSQRTLRQLTANTGHTIHVHYPGN----RQPNPPLILQRLLGPSAAADILQLSSSLPLQSRGRARLLVGNDDVHIIARSDDELLDDFFHDQSTATSQAG---TLSSIPTALTRWTEECKVLDAESMHDCVSVVKVPIVNHLEFLRDEELEERREKRRKQLAEEETKIIDKGKEDKENRDQS-AQCTVTKTNDSTEQNVSDG-------TPMPDSYP----------------TTPSSTDAPTSESKETLGTLQPSQQQPALPPPPSLGEIPQELQSPAEEVANSTQLLMPIELEELGPTRPSGEAETTQMELSPAPTITSLSPERAEDSDALTAVSSQLEGSPMDTSSLASCT--------------LEEAVGDTP--AAGSSEQPTAGSSTPGDAPSVVAEVQGRP--DVSRESNQPPEDSSPPASSESSSTRDSAVAISGADSRGILEEPLPSTSSEEEDP-----LAGISLPEGVDPSFLAALPDDIRREVLQNQLGIRPPTRSAPSSNSSAPAVVGNPGVTEVSPEFLAALPPAIQEEVLAQQRAEQQRRELAQNASSDTPMDPVTFIQTLPSDLRRSVLEDMEDSVLAVMPPDIAAEAQALRREQEARQRQLMHERLFGHSSTSALSAILRSPAFTSRLSGNRGVQYTRLAVQRGGTFQMGGSS---------SHNRPSGSNVDTLLRLRGRLLLDHEALSCLLVLLFVDEPKLNTSRLHRVLRNLCYHAQTRHWVIRSLLSILQRSSESE 3237 HSP 5 Score: 68.5514 bits (166), Expect = 1.089e-9 Identity = 53/147 (36.05%), Postives = 74/147 (50.34%), Query Frame = 0
Query: 2239 KGTGSKEESGSGNN------LPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDL---------------QNELLSIKSTNFEHDRSGKDKGGLLDRF-TKETVVLNAPSKQ 2363
KG+ S + G G + + ++ + L+L+V+VLTS SCSEEGLED +LL LS G TRD +L+LLL GA+ LG + + L Q E LS E ++ K KG + RF E VV+ A K+
Sbjct: 3553 KGSKSPAKVGEGGSGIDFKMVSSGLTENQLQLSVEVLTSHSCSEEGLEDAANVLLQLSRGDSGTRDTVLKLLLNGARHLGYTLCKQIGTLLAELREYNLEQQRRAQCETLSPDGLPEEQPQTTKLKGKMQSRFDMAENVVIVASQKR 3699
BLAST of EMLSAG00000004991 vs. SwissProt
Match: gi|50403677|sp|P51593.2|HUWE1_RAT (RecName: Full=E3 ubiquitin-protein ligase HUWE1; AltName: Full=HECT, UBA and WWE domain-containing protein 1; AltName: Full=HECT-type E3 ubiquitin transferase HUWE1; AltName: Full=Upstream regulatory element-binding protein 1; Short=URE-B1; Short=URE-binding protein 1) HSP 1 Score: 568.926 bits (1465), Expect = 0.000e+0 Identity = 259/317 (81.70%), Postives = 288/317 (90.85%), Query Frame = 0
Query: 2637 EEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
EEGQDAGGLLREWY+IISRE+FNPMYALF+TSPGD+VTY I SS NPNHL YFKFVGR++AKA+YDN+LLECYFTRSFYKHILGK V+YTDMESEDY FY+GL +L+ + VS LGYDLTFSTE+QEFGV E+RDL PNG NI VTEE K +Y+ LVCQM+MTGAIRKQL+AFLEGFY IIP+RLISIF EQELELL+SGLPTIDIDDLKSNTEYHKYQ NS+QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE+++KLR LL AI ECSE
Sbjct: 1 EEGQDAGGLLREWYMIISREMFNPMYALFRTSPGDRVTYTINPSSHCNPNHLSYFKFVGRIVAKAVYDNRLLECYFTRSFYKHILGKSVRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMTGAIRKQLAAFLEGFYEIIPKRLISIFTEQELELLISGLPTIDIDDLKSNTEYHKYQSNSIQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYESFEKLRHMLLLAIQECSE 317
BLAST of EMLSAG00000004991 vs. SwissProt
Match: gi|597439636|sp|Q9P4Z1.4|TOM1_NEUCR (RecName: Full=E3 ubiquitin-protein ligase TOM1-like; AltName: Full=HECT-type E3 ubiquitin transferase TOM1-like) HSP 1 Score: 462.225 bits (1188), Expect = 5.768e-129 Identity = 220/422 (52.13%), Postives = 292/422 (69.19%), Query Frame = 0
Query: 2539 FLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRRE--DLAVHVRRENVFEESFRELXRRSPEEXK-NRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
F F E HR +LN+++R + L G FS+LV + +VL+FD KR YF + ++ R L + VRRE+VF +SFR L + +E K + I F+GEEG DAGG+ REW+ ++SR++F+P Y LF D+ T+ + S N HL +FKF+GR+I KA+Y+ +LLECYF+R+ YK ILGKPV DMES D +YK L +++ + ++D+ + TFS E FG ++ DLI NGRNI VTEE K +Y+RL+ + K+ +++ Q+ AFL GF+ IIP LI+IFNEQELELL+SGLP IDIDD K+NTEYH Y + QI WFWRA+RSFD+ + AK +QFVTG+SKVPL GF LEGMNGV +F IHRD S DRLPS+HTCFNQLDLP YE Y+ LR+ LLKAI S+ F
Sbjct: 3656 FFTFTEEHRRILNELVRHN-PKLMSGTFSLLVKNPKVLEFDNKRNYFNRSVHSKYQQTRHSFPPLQLQVRREHVFHDSFRSLYYKKADELKFGKLNIRFQGEEGVDAGGVTREWFQVLSRQMFDPNYVLFVPVSSDRTTFHPNKLSPINDEHLPFFKFIGRIIGKALYEGRLLECYFSRAVYKRILGKPVSVKDMESFDPDYYKSLVWMLENDITDIITE-TFSVEDDVFGEVKVVDLIENGRNIPVTEENKHEYVRLIVEHKLITSVKDQMKAFLTGFHEIIPEELIAIFNEQELELLISGLPDIDIDDWKANTEYHNYSAGAPQIQWFWRAVRSFDKEELAKLLQFVTGTSKVPLNGFKELEGMNGVSRFNIHRDYGSKDRLPSSHTCFNQLDLPEYENYETLRSQLLKAITAGSDYFGF 4075 HSP 2 Score: 94.3597 bits (233), Expect = 1.528e-17 Identity = 91/292 (31.16%), Postives = 136/292 (46.58%), Query Frame = 0
Query: 1902 GVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQ-NTEGSNVPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQ-------VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSF-QLPNKSKR 2184
G+D +L ALPEE R EVI + + R +A++Q + EG N EV EFL ALP ++ E+L Q++ EQ+R+ Q + P A +DP T P LRQ VL D + M L ELAAEA++L Q +H + GG + S R+A R TEA A + + ++ Q+LD +G++ LL L+FV + L I NLC + R V+ L+ IL+ D +++ SF Q+ +K+K+
Sbjct: 2975 GIDAEYLEALPEEFRDEVI--AQAISTRRSQAREQVSQEGENT-EVFQEFLEALPEELRNEILHQEQHEQRRRERQNAAGGQDLGP-ADMDPASILLTFPPGLRQQVLLDQGEDIMEHLGPELAAEARTLVARHRQ-----LHAQ---QGGQAASR-------------------SRDA-----QRPTEAGAGTVQKIQKRTVV----------QMLDKQGIATLLRLMFVSQQGSIRSSLFSIFANLCENRQNRLDVISSLLQILQDGCANMD---AVERSFAQISHKAKQ 3217
BLAST of EMLSAG00000004991 vs. SwissProt
Match: gi|442570058|sp|Q756G2.2|TOM1_ASHGO (RecName: Full=Probable E3 ubiquitin-protein ligase TOM1; AltName: Full=HECT-type E3 ubiquitin transferase TOM1) HSP 1 Score: 434.491 bits (1116), Expect = 1.027e-120 Identity = 225/516 (43.60%), Postives = 323/516 (62.60%), Query Frame = 0
Query: 2440 LSEQLSLDSLWEALGDCLKDLAETPDHH-AVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXK-NRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
L ++ L +W +L +CL E P + +L +E+ +V S + K + + E ++ A +P F AF + H+ +LN+++R S L GPFS+LV + ++LDFD KR YF +L + R L++ VRRE+VF +S+R L +S E+ K ++ I F+GE G DAGG+ REWY ++SR++FNP YALF DK T+ +S NP HL +FKF+G +I KAI D L+C+F+R YK+ILGKPV DMES D +YK L +++ + ++D+ + TFS E ++G ++ +LI NG ++ VTE+ K DY++ + + K+ +++ Q+ FL+GFY IIP+ LISIF+EQELELL+SGLP ID+DD K+NT Y Y QI +FWRA+RSFD+ ++AK +QFVTG+SKVPL GF L G+NG+ KF IHRD S DRLPS+HTCFNQLDLPAY++Y+ LR LL AI+E E
Sbjct: 2767 LYNKMELGHIWVSLSNCLTRFEEKPRMSTSATILLPLIESLMVVCKHSKVRETKDALLKYEAKKCDFAR-------------------------TPVENLFFAFTDLHKKLLNEMIR-SNPKLMSGPFSLLVKNPKILDFDNKRYYFTAQLRAITHD--RPKLSISVRREHVFLDSYRSLFFKSNEDIKISKLEISFKGEAGVDAGGITREWYQVLSRQMFNPDYALFIPVASDKTTFRPNRTSGINPEHLSFFKFIGMIIGKAISDQCFLDCHFSREVYKNILGKPVALKDMESLDLDYYKSLIWILENDITDI-IEETFSVETDDYGEHKVIELIENGAHVAVTEQNKHDYVKKIVEYKLQTSVKDQMENFLQGFYAIIPKDLISIFDEQELELLVSGLPDIDVDDWKNNTIYVNYTPTCKQINYFWRAVRSFDKEERAKLLQFVTGTSKVPLNGFKELSGVNGISKFSIHRDYGSIDRLPSSHTCFNQLDLPAYDSYETLRGSLLLAINEGHE 3253
BLAST of EMLSAG00000004991 vs. SwissProt
Match: gi|172045749|sp|Q8GY23.3|UPL1_ARATH (RecName: Full=E3 ubiquitin-protein ligase UPL1; Short=Ubiquitin-protein ligase 1; AltName: Full=HECT-type E3 ubiquitin transferase UPL1) HSP 1 Score: 428.713 bits (1101), Expect = 7.085e-119 Identity = 235/544 (43.20%), Postives = 330/544 (60.66%), Query Frame = 0
Query: 2444 LSLDSLWEALGDCLKDLAETPDHHA----------VLVLQSS-------------VEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQE-----FGVTEIRD--LIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
++L+ LW L C+ DH A VL + + +EAFF++ +K+Q Q Q ++ SS+ +S S + + F FAE HR +LN +RQ+ + L + S+++ R++DFD K+ YFR+ + ++ L + VRR V E+S+ +L RSP++ K R + F+GEEG DAGGL REWY ++SR IF+ LF T+ G+ T+ +S HL YFKFVGR++AKA++D +LL+ YFTRSFYKHILG V Y D+E+ D +YK L++L+ + VSD+ DLTFS + E + TE+ D L P GRNI VTEETK +Y+ LV +T AIR Q++AFLEGF +IPR L+SIFN++ELELL+SGLP ID DDLK+NTEY Y S I WFW +++F + D A+F+QFVTG+SKVPL+GF L+G++G Q+ QIH+ + +RLPSAHTCFNQLDLP Y++ ++L+ LL AIHE SE F
Sbjct: 3150 VALEPLWHELSQCISMTELQLDHTAAASNINPGDHVLGISPTSSLSPGTQRLLPLIEAFFVLC---------EKIQTPSMLQ-QDTNVTAGEVKESSAHGSSSKTSVDSQKKTDGSVTFSKFAEKHRRLLNSFIRQNPS-LLEKSLSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRISVRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLF-TTVGNDATFQPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNLKWLLENDVSDI-LDLTFSMDADEEKHILYEKTEVTDYELKPGGRNIRVTEETKHEYVDLVAGHILTNAIRPQINAFLEGFNELIPRELVSIFNDKELELLISGLPEIDFDDLKANTEYTSYTAGSPVIHWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGISGPQRLQIHKAYGAPERLPSAHTCFNQLDLPEYQSKEQLQERLLLAIHEASEGFGF 3680 HSP 2 Score: 98.9821 bits (245), Expect = 6.843e-19 Identity = 97/275 (35.27%), Postives = 141/275 (51.27%), Query Frame = 0
Query: 1896 DVEIPE--GVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFK---GRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANET 2165
D PE +DP+FL ALPE++R EV+ Q+ Q Q E V +++PEFLAALPP+IQ EVLAQQR ++ Q SQ +D TLPA LR+ VL ++ +AALP+ L AEAQ LR +R H + + SS+ GS RL N+ N +G RL+G +T +R + A+ ++ K G L++ + L L+ LL + + L R+L NLC H+ TR +V+ L+ ++ ET
Sbjct: 2600 DGRAPEVNAIDPTFLEALPEDLRAEVLASQQA-----QSVQPPTYEPPPVDDIDPEFLAALPPDIQTEVLAQQRAQRMVQQSQ-GQAVDMDNASIIATLPADLREEVLLTSSEAVLAALPSPLLAEAQMLR------DRAMSHYQ-------ARSSVF-GSSHRLNNRR---NGLGYN--RLTGMD----RGVGVTIGQRAVSSSADGLKVKEIEGDPLVNADALKSLIRLLRLA-QPLGKGLLQRLLLNLCAHSFTRANLVQLLLDMIRPEMET 2844 HSP 3 Score: 75.8702 bits (185), Expect = 5.898e-12 Identity = 44/132 (33.33%), Postives = 69/132 (52.27%), Query Frame = 0
Query: 804 ALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTSKEI-NYILRVLAPAACRSPSIFVSVAKDILRFEPS 934
ALL AC G + V + + AVL+LC RLT+ A++F GG+ L L + F G ++A+++VRH++EDP TL+ ME IR + + + + L +AP R P +F+ + E S
Sbjct: 1553 ALLIAC-GLIKQHVPAMIMQAVLQLCARLTKSHALAIQFLENGGLSSLFNLPKKCCFPGYDTVASVIVRHLVEDPQTLQIAMETEIRQTLSGKRHIGRVLPRTFLTTMAPVISRDPVVFMKAVASTCQLESS 1683
BLAST of EMLSAG00000004991 vs. SwissProt
Match: gi|332278235|sp|Q8H0T4.3|UPL2_ARATH (RecName: Full=E3 ubiquitin-protein ligase UPL2; Short=Ubiquitin-protein ligase 2; AltName: Full=HECT-type E3 ubiquitin transferase UPL2) HSP 1 Score: 428.713 bits (1101), Expect = 7.150e-119 Identity = 244/576 (42.36%), Postives = 346/576 (60.07%), Query Frame = 0
Query: 2418 VDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVL--------VLQSS---------------VEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMESSSTASSCSSNSLSPDTKK------FLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQE-----FGVTEIRD--LIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+D+++ + E Q ++ L +++L+ LW+ LG C+ DH A VL S +EAFF++ +K+Q Q Q+ E++ SST S SS ++ KK F F E HR +LN +RQ+ + L + FS+++ R++DFD K+ YFR+ + ++ L + VRR V E+S+ +L RSP++ K R + F+GEEG DAGGL REWY ++SR IF+ LF T+ G+ T+ +S HL YFKFVGR++AKA++D +LL+ YFTRSFYKHILG V Y D+E+ D +YK L++L+ + VSD+ DLTFS + E + TE+ D L P GRNI VTEETK +Y+ LV +T AIR Q++AFLEG +IPR L+SIFN++ELELL+SGLP ID DDLK+NTEY Y S I WFW +++F + D A+F+QFVTG+SKVPL+GF L+G++G Q+ QIH+ S +RLPSAHTCFNQLDLP Y++ ++++ LL AIHE +E F
Sbjct: 3103 IDDSNVGTDKETDQEEQNIMQGL--KVALEPLWQELGQCISMTELQLDHTAATSNVNPGDHVLGISPTSSLSPGTQSLLPLIEAFFVLC---------EKIQTPSMLQ------QDATVTAGEVKESSTHGS-SSKTIVDSQKKIDGSVTFSKFVEKHRRLLNSFVRQNPS-LLEKSFSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRISVRRAYVLEDSYNQLRMRSPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLF-TTVGNDATFQPNPNSVYQTEHLSYFKFVGRMVAKALFDGQLLDVYFTRSFYKHILGVKVTYHDIEAVDPDYYKNLKWLLENDVSDI-LDLTFSMDADEEKHILYEKTEVTDYELKPGGRNIRVTEETKHEYVDLVADHILTSAIRPQINAFLEGLNELIPRELVSIFNDKELELLISGLPEIDFDDLKANTEYTSYTVGSPVIRWFWEVVKAFSKEDMARFLQFVTGTSKVPLEGFKALQGISGPQRLQIHKAYGSPERLPSAHTCFNQLDLPEYQSKEQVQERLLLAIHEANEGFGF 3657 HSP 2 Score: 93.2041 bits (230), Expect = 3.729e-17 Identity = 104/327 (31.80%), Postives = 151/327 (46.18%), Query Frame = 0
Query: 1851 DVSMESDQPRESEEAIGGXSXIASSNPIPSTSGTS-----QNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLR----REXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFK---GRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANET 2165
DV M SD E +++ +N + S T + D S+ G +DP+FL ALPE++R EV+ Q+ Q Q E +V +++PEFLAALPP IQ EVLAQQR ++ Q SQ P +D TLPA LR+ VL ++ +AALP L AEAQ LR R + R+R F NN N L +RL+G +T +R + A+ ++ K G L++ + L L+ LL + + L R+L NLC H+ TR +V+ L+ ++ ET
Sbjct: 2537 DVDMSSDGA-EGNQSVQPSPLDGDNNELSSMEATQDVRNDEQVDEGSLDGRAPEVNAIDPTFLEALPEDLRAEVLASQQA-----QSVQPPTYEPPSVDDIDPEFLAALPPEIQREVLAQQRAQRMLQQSQGQP-VDMDNASIIATLPADLREEVLLTSSEAVLAALPPPLLAEAQMLRDRAMRHYQARSRVFGSSHRLNNRRNGL------------------------GYRLTGME----RGVGVTIGQRDVSSSADGLKVKEIEGDPLVNADALKSLIRLLRLA-QPLGKGLLQRLLLNLCAHSFTRANLVQLLLDMIRPEMET 2827 HSP 3 Score: 76.2554 bits (186), Expect = 5.286e-12 Identity = 43/139 (30.94%), Postives = 70/139 (50.36%), Query Frame = 0
Query: 797 LKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTSKEI-NYILRVLAPAACRSPSIFVSVAKDILRFEPS 934
L +E S +L G + V + + AVL+LC RLT+ A++F GG+ L L + F G ++A+++VRH++EDP TL+ ME IR + + + + L +AP R P +F+ + E S
Sbjct: 1547 LTMEESSKVLLIACGLIKQRVPAMIMQAVLQLCARLTKSHALAIQFLENGGLSSLFNLPKKCFFPGYDTVASVIVRHLVEDPQTLQIAMETEIRQTLSGKRHIGRVLPRTFLTTMAPVISRDPVVFMKAVASTCQLESS 1685
BLAST of EMLSAG00000004991 vs. SwissProt
Match: gi|50400805|sp|O13834.1|PTR1_SCHPO (RecName: Full=E3 ubiquitin-protein ligase ptr1; AltName: Full=HECT-type E3 ubiquitin transferase ptr1; AltName: Full=Poly(A)+ RNA transport protein 1) HSP 1 Score: 424.476 bits (1090), Expect = 1.369e-117 Identity = 223/518 (43.05%), Postives = 319/518 (61.58%), Query Frame = 0
Query: 2442 EQLSLDSLWEALGDCLKDLAE-TPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXK-NRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+ L SLWE L +CL L + T H VL +E+ ++ ++ E ++ HI L K + F++F E HR ++N ++ +T L G FS+LV + +VL+F+ KR YF +L + + L + VRR++VF +S+R L + +E K ++ I F EEG DAGG+ REW +++R++FNP YALF GD T+ S NP+HL +FKF GR+I KA+YD +LL+C+F+R+ YKH+L + V D+ES D +YK L +++N+ ++D+ + F+ E FG + DLIPNGRNI VTE K +Y+ + K+ +++ QL + L+GF +IIP LI IFNEQELELL+SGLP IDIDD K+NTEYH Y +S Q+ WFWRA+RSFD+ ++AK +QF TG+SKVPL GF LEGM+G Q+F IH+ S +RLP +HTCFNQLDLP Y+TY++LR+ LL AI+E SE F
Sbjct: 2741 DNLKFSSLWEVLSECLSALRDHTSITHVSTVLLPLIESLMVI----------CRLVFIELPEDVGKHISPILERFKTL--------------------FISFTEEHRKIINMMVF-TTPSLMSGSFSLLVKNPKVLEFENKRNYFNRQLHEEAAKEQYPPLNITVRRDHVFLDSYRALHFKDADEVKFSKLNIHFRDEEGVDAGGVTREWLQVLARQMFNPDYALFLPVTGDATTFHPNRDSSVNPDHLSFFKFTGRIIGKALYDGRLLDCHFSRAVYKHMLHRSVSVKDIESLDPDYYKSLVWMLNNDITDIITE-EFAVEKDVFGEKTVVDLIPNGRNIPVTELNKQNYVNRMVDYKLRESVKDQLKSLLDGFSDIIPSHLIQIFNEQELELLISGLPEIDIDDWKNNTEYHGYNVSSPQVQWFWRAVRSFDEEERAKLLQFATGTSKVPLNGFKELEGMSGFQRFNIHKSYGSLNRLPQSHTCFNQLDLPEYDTYEQLRSMLLTAINEGSEGFGF 3226
BLAST of EMLSAG00000004991 vs. SwissProt
Match: gi|50401412|sp|Q03280.1|TOM1_YEAST (RecName: Full=E3 ubiquitin-protein ligase TOM1; AltName: Full=HECT-type E3 ubiquitin transferase TOM1; AltName: Full=Suppressor of snRNA protein 2; AltName: Full=Temperature-dependent organization in mitotic nucleus protein 1) HSP 1 Score: 414.075 bits (1063), Expect = 1.916e-114 Identity = 203/416 (48.80%), Postives = 288/416 (69.23%), Query Frame = 0
Query: 2539 FLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKN-RFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE 2953
F F + H+ +LNQ++R S L GPF++LV + +VLDFD KR +F +L+ ++ R L + VRRE VF +S+R L ++ +E KN + I F+GE G DAGG+ REWY ++SR++FNP YALF P DK T+ +S NP HL +FKF+G +I KAI D L+C+F+R YK+ILG+PV DMES D +YK L +++ + ++D+ + TFS E ++G ++ +LI G++I VTE K DY++ V + K+ ++++Q+ FL GFY +I + LI+IF+EQELELL+SGLP ID+DD K+NT Y Y ++ +FWRA+RSFD ++AK +QFVTG+SKVPL GF L G+NGV KF IHRD S++RLPS+HTCFNQL+LP YE+Y+ LR LL AI+E E
Sbjct: 2852 FFPFTDAHKKLLNQMIR-SNPKLMSGPFALLVKNPKVLDFDNKRYFFNAKLKSDNQE--RPKLPITVRREQVFLDSYRALFFKTNDEIKNSKLEITFKGESGVDAGGVTREWYQVLSRQMFNPDYALFLPVPSDKTTFHPNRTSGINPEHLSFFKFIGMIIGKAIRDQCFLDCHFSREVYKNILGRPVSLKDMESLDPDYYKSLVWILENDITDI-IEETFSVETDDYGEHKVINLIEGGKDIIVTEANKQDYVKKVVEYKLQTSVKEQMDNFLVGFYALISKDLITIFDEQELELLISGLPDIDVDDWKNNTTYVNYTATCKEVSYFWRAVRSFDAEERAKLLQFVTGTSKVPLNGFKELSGVNGVCKFSIHRDFGSSERLPSSHTCFNQLNLPPYESYETLRGSLLLAINEGHE 3263
BLAST of EMLSAG00000004991 vs. SwissProt
Match: gi|1008808725|sp|G0S9J5.2|RSP5_CHATD (RecName: Full=E3 ubiquitin-protein ligase RSP5; AltName: Full=HECT-type E3 ubiquitin transferase RSP5) HSP 1 Score: 335.109 bits (858), Expect = 5.026e-96 Identity = 160/375 (42.67%), Postives = 241/375 (64.27%), Query Frame = 0
Query: 2577 DFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHEC 2951
DF K YFR++ R+ + VRR ++FE++F E+ R+S + K R I F+GE+G D GGL RE++ ++S E+FNP Y LF+ S D T I S NP HL+YFKF+GRV+ AI+ + L+ +F +FYK ILGKPV DME D F++ L++++++ +S + TFSTE + FGV + DL PNGRNI VT E K +Y+ L+ + ++ + +Q AF EGF +IP+ LI++F+E+ELELL+ G+ ID+DD K +T+Y Y + I +FW+ +RS+D K++ +QF TG+S++P+ GF L+G +G ++F I R + LP AHTCFN+LDLP Y+T ++L+ L A+ E
Sbjct: 444 DFRRKLIYFRSQPAM---RILSGQCHIKVRRSHIFEDAFAEISRQSATDLKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCLFEYSAHDNYTLQINPHSGINPEHLNYFKFIGRVVGLAIFHRRFLDAFFITAFYKMILGKPVTLADMEGVDADFHRSLQWMLDNDISGGIIEATFSTEDERFGVITVEDLKPNGRNIEVTNENKREYVELMVKWRIQKRVEEQFKAFKEGFNELIPQDLINVFDERELELLIGGIAEIDVDDWKKHTDYRGYTESDEVIQFFWQTVRSWDSEQKSRLLQFTTGTSRIPVNGFKDLQGSDGPRRFTIERAG-DINNLPKAHTCFNRLDLPPYKTLEQLQQKLTMAVEET 814
BLAST of EMLSAG00000004991 vs. Select Arthropod Genomes
Match: gb|KPM07940.1| (E3 ubiquitin-protein ligase HUWE1-like protein [Sarcoptes scabiei]) HSP 1 Score: 980.704 bits (2534), Expect = 0.000e+0 Identity = 541/1068 (50.66%), Postives = 710/1068 (66.48%), Query Frame = 0
Query: 1892 SILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQ---NIRQ--RAQQQNTEGSNV---PEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSV-SRL-GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKIN-TTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNK--SKRSSTATPVK-SASSQQQQQXKYPVIRRNSLLTDKLLRLLSLI--SIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHL-RLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANV----LDLQNELLSIKS--------TNFEHDRSGKDKGGLLDRFTKETVVLNAPSKQXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEENKMDVDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQ-----EQLADNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHT 2924
+ILGD E+PEGVDPSFLAALPE +RQEVI +Q RLQ N+R A Q N SN EV+PEFLAALPPNIQEEVLAQQR EQQR +Q NP+APVDP F +LP +LR+ VL D++DSQ+ LP +LA EA+SLRR+ E RNR MHERFF N++S I+R + SR+ + + NL R +F + G + + ++TR L N FKG+ LLD +GL+ ++ILLF++++ IN +++ IL P + ++ + +E+ + + + +K ++ P++ S +Q P +++N +LTD+L+RL+ I S R ++ + S E ++ IS + L +L VDVL +K S EG+ D +AL++ L + R I+ + LL+G + LG +V + ++++ L+ IKS + FE ++ K K + DR++K T+++ + S + +E + P+ S + +++ I + + + +NSS+++ E + +E L LD LWE L +CL L+E PD H VLVLQ +VE+FFLVHA Q K + + E Q++HI E LA + +++ S SL +T+KFL FAE HR VLNQILRQS HL +GPFSVLVDHTR+LDFD+KRRYFR ELER+D+ +RR+DL VH+RRE++FE+S+REL RR+ + KNRFY+VFEGEEGQDAGGLLREWY IISREIFNP YALF TSPGD+VTYMI +S N NHL YFKFVGRVI+KAIYDNKLL+CYFTRSFYKHILGK VKYTDMESEDYAFY+GL FL+ H V++LG +LTFS EIQEFGVTEIRDL+P+GRNI VTEE K +Y++LVCQ KMTG+I+KQL +FLEGFY IIP+ LISIFNE ELELL+SGLPTIDIDDLK+NTEYHKYQ NSLQI WFWRALR+FDQ D+AKF+QFVTG+SKVPLQGF+ LEGMNG QKFQIH DDRSTDRLPSAHT
Sbjct: 2295 AILGDQELPEGVDPSFLAALPENIRQEVIAEQFRLQRLNNLRTLTTANQSNASSSNAVQNAEVSPEFLAALPPNIQEEVLAQQRAEQQRLNAQNTNPDAPVDPVSFLNSLPPTLRRQVLTDLDDSQVQLLPPDLANEARSLRRDYEMRNRQ-MHERFFT-TNNTISRIIRSAANSRVRYDPFNFQNL--RLSFAIRGGSYDRDNFQSLTRNVPRP--LTNR-SFKGKHLLDFDGLASVMILLFLNENVINKSSKTQSIL-------PITNHILSSRNNQIEQVLKYSLFFGEFIFHLEFLDNQITKVGQSSKPLELSPLTQLLNLLSSPFVKKNRVLTDRLIRLVGFIITSFIRDQPEGQRNQQPIDLSNEIKEKLKDIEQSISDERLLKLVVDVLITKPYSTEGISDSSALIVKLCNLFPSCRSILYKFLLEGVRNLGEIVYKEINTLIFEMKDLLIKIKSQKTIDTLDSTFE--KTIKGKSNIQDRYSKMTIIVKSQSNLKQITGKEVQ-------LPSMSNLISKSSSQFLFCRILKIILHIRENNAKAQQSQAAKNSSETSMEVDNEDKLCVE-----LQLDHLWEKLSECLTLLSEAPDDHVVLVLQPAVESFFLVHA---QEKSKNQTKANEPVNVQVSHINQAGQSEVLATDSNVDNESRQYG----SLPFETQKFLIFAEKHRVVLNQILRQSNVHLMNGPFSVLVDHTRILDFDVKRRYFRQELERLDQNIRRDDLPVHIRREHIFEDSYRELHRRTCNDWKNRFYVVFEGEEGQDAGGLLREWYTIISREIFNPNYALFTTSPGDRVTYMINSASHCNSNHLSYFKFVGRVISKAIYDNKLLDCYFTRSFYKHILGKAVKYTDMESEDYAFYQGLVFLLEHNVNELGTELTFSLEIQEFGVTEIRDLVPDGRNIIVTEENKHEYVKLVCQEKMTGSIKKQLHSFLEGFYEIIPKHLISIFNEHELELLISGLPTIDIDDLKANTEYHKYQINSLQIQWFWRALRTFDQADRAKFLQFVTGTSKVPLQGFSALEGMNGPQKFQIHLDDRSTDRLPSAHT 3327 HSP 2 Score: 378.252 bits (970), Expect = 7.427e-104 Identity = 285/905 (31.49%), Postives = 452/905 (49.94%), Query Frame = 0
Query: 382 EPAPGFSFYDLQILRDMGFSRERCLEAMDRTGA-MYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSPIEEDVFRTTESNKAAV----RIHLFILLFEECKLLCAKIVEESSVVPIM-----------THLLTVSFDVLKRCSGSVGTPKWMTPLLLFIDLYEKVILGMNRRASL---TSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK-SKGSRDAVVKSSATSSNSNNAMELGEEE-----KKVSDPNE--EDVITTIPTA-----EGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLVKTPDICESSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKDMKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSKA---DKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSS 1250
+PA +++L +MGF + L+A G + A +++L S N+ E ++D+++AIA+SL +D E D + + K +N L+ M+ L L L+D +PE V + C+L++A RNGKE+ E+I+ LI + + I+ L K + + S D ++K A+ RIHL ++L+ E K K + +V PI+ ++LL VSF+ ++ TPKW+ P +LFIDLYE + ++RR L T R WK+++ + RW Y P NK+IDD+F +GE RI RRKYTI FG+M+Q N+ET N RP+M+ I+ K + ++ +K+ + N E +E KK+ D E ++ T++ T+ + L + + +L+++ + IP++ + LNA+LRL LRLT+ +E A F GV LLL++ QSS+F G SL L++RHVLED +L+ TME++I NS +S E+NY +R +PAACR+ ++F AK++ RF P P N ++ + L S + + A + + S+ VI DLLN L + NA +ES C S++ K V +L EL +SY AKLI + Y G++E++ E SA++F+L++LL+ A DKD + L+A++AS PE L E+K AL +++ PES KH+KIQA+ LI+ M E S I + F ++ K+++K+ L D+A++ + +DLSSP I+ TIN+ LK LE LS+ N P L+ S
Sbjct: 1183 QPAQAPEHAGVRVLIEMGFPPDLALDAFFNVGDDVTAAADWILANHSLSLNSVNE-------------------EDDMIKAIALSL-LD----------SSGGESDEKIKKEKICHKNYHQLIPLTSDEMNRLMDNILAKILILIDQVPEIVNKCCELMVAFSNRNGKEWFEEIINNLIIDTITIIEQLNKKTFYKQDNDSGTTTDWSAELSTSKEAIQLSSRIHLLLVLYNEAK----KNFSQEAVNPIIIDQLISLLENSSYLLNVSFEK----QSTITTPKWIAPAVLFIDLYEHYAVALDRRNLLLAETMNAKRVWKYFEDRAQRWNSYQPDINKSIDDSFRNGELYKRINANRRKYTINFGSMVQINDETCNLRPVMLFIEPDKPTVNSTIKNEMNERDLNVEREKPNQENDFLSKKIDDEYESKKNSKTSVATSYKSIFKLLNSKQSKSLIESSINLFKIPIEPETLNAILRLNLRLTRNYENASYFVEKNGVNLLLKIPQSSVFCGFVSLVILIIRHVLEDEKSLQTTMERLICNFNQNS-NSSHEVNYQIRFYSPAACRNANLFKISAKNLFRFNVPVPSNSNRRSNSENTLRTDSKLSLDIWNNADKIPKPSKDVISDLLNMLPMKYAQQQQRKNAQ-LESG---------------------------------------CLNEKSEEKCKDVFHVHTVLSILDELTRSYHIVAKLICEFNYPIGLIESMVE-CSAISFILNKLLSHNANYGDKDCATYSRALLASIASVPFTPEVHHTLFNEIKSALKNTILLPESVEKHAKIQAMMTLIA-MFEMPSSLIHPYRANNFTN------SSFFKLLVKKNLAADVAKLINHMDLSSPMISNTINTLLKTLEILSKNHNYPNNLLGS 2000 HSP 3 Score: 166.392 bits (420), Expect = 1.298e-39 Identity = 76/135 (56.30%), Postives = 95/135 (70.37%), Query Frame = 0
Query: 27 HHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPA---APSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWAL 158
HH K IKPL+ +SRLGRALAELF LLVKL +G PIR RR H P P+ P+ PA ++ LN+LLA GLSW P + +P RLTFF CS+GF+ PMLFDD+K+PYHLML FV S GL++FF+ + A+
Sbjct: 984 HH--KCIKPLITVASRLGRALAELFGLLVKLSIGLPIRNRRNHHQPNFPSHFNLPTLPAQRISKHLNKLLARGLSWKPSFKTDVPKIRLTFFVCSIGFSSPMLFDDRKFPYHLMLKNFVLSNGLESFFECYETAI 1116
BLAST of EMLSAG00000004991 vs. Select Arthropod Genomes
Match: EFX86378.1 (hypothetical protein DAPPUDRAFT_313219 [Daphnia pulex]) HSP 1 Score: 966.837 bits (2498), Expect = 0.000e+0 Identity = 495/776 (63.79%), Postives = 577/776 (74.36%), Query Frame = 0
Query: 2261 HLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIK---------------STNFEHDRSGKD--KGGLLDRFTKETVVLNAPSKQXKKDMQ--------------------------------------EAKKSSPPSDTPAESTTTNTTTAPASTTTS------TSDTTTIADQTNEENKMDVDENSSDST----SEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEK-KKVQQKETRQEQLAHIQEQLA-------------DNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
HL+LAVDVLTSKSCSEEGLED T+LLL+LS+GP PTRD IL+LLL GA+ELGN+V +++ L NEL +++ +T+ E D G D KG L+DRFTK+ VV++A +K + +AK+ + TPA + ++ A T S SD+ T A + N +T E ++ LS+QL LDSLW L +CL++LA+TPDHHAVLVLQ +VEAFFLVHAA T +E+ K+ ++ ETR+ QL+H+Q+++ D+ + + + SS S+ L PDT+KFL+FAE HRTVLNQILRQST HLADGPF+VLVDHTRVLDFD+KRRYFRTELER D +RREDLAVHV+RE+VFE+SFREL RRSPEE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF TSPGD+VTYMI S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGK V+YTDMESEDY+FY+GL FL+ H+V++LGYDLTFSTE+QEFGVTE RDL PNGRNI VTEE K++YIRLVCQMKMTGAIRKQL+AFLEGFY+IIP+RLISIFNEQELELLLSGLP IDIDDLK+N+EYHKYQ SLQI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF+ LEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE F
Sbjct: 3597 HLKLAVDVLTSKSCSEEGLEDATSLLLSLSYGPHPTRDTILKLLLDGARELGNIVCSHIRALLNELHTLRRRPEASTSAVSAQTGTTSEETDLEGVDRSKGFLMDRFTKDMVVVSAQTKPKTGGYELQLPSMGSLTSKTSSQAFFLRILKVILQLRDAARLAAVKAKRKQVEATTPAPAPADGSSVAAVGTEPSEPVIPEVSDSATPASAPSTAGAAAEAPNEVPATPMEVDEVGGGRDIELPRLSQQLLLDSLWNTLSECLQELADTPDHHAVLVLQPAVEAFFLVHAAQTSKEEREKRSRRPETRESQLSHLQQEIGGPGSPSMAAQASNDDSLNQLTLSMSSAPSSDLPPDTQKFLSFAERHRTVLNQILRQSTTHLADGPFAVLVDHTRVLDFDVKRRYFRTELERADAGIRREDLAVHVKREHVFEDSFRELHRRSPEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFSTSPGDRVTYMINALSHCNSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKCVRYTDMESEDYSFYQGLAFLIEHEVTELGYDLTFSTEVQEFGVTEARDLKPNGRNIIVTEENKMEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLLSGLPNIDIDDLKANSEYHKYQPTSLQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFSALEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEGFGF 4372 HSP 2 Score: 699.123 bits (1803), Expect = 0.000e+0 Identity = 459/1088 (42.19%), Postives = 623/1088 (57.26%), Query Frame = 0
Query: 392 LQILRDMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNN-EGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSPIEEDVFRTTESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVL------------KRCSGSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIKSKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPNEEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTS-KEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLV----------KTPDICESSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTIT----TAQPCDTSASKDM---KKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTS---KADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQP---AFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSARKSKNDVPDSSAEDSTSNNPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDE-SVEPDAEATEHDVSTTAESI------DPNSESQLQTVEDTNDEDYDEMMDQL---------LERDGS--DNFTIIPQVGSNVNSDQDHHQDG----------SRFAGHGCQDDVDRAXESSQXDAAIEEETED 1414
LQ L DMGF R++C+EA+ T ++ +AT+YLL+ S G + A A + +++ +MRAIA+SL D ++ D KE +E+ LSK V+D+F QQAL GCL+L+D+LPETV+R C L++A+ RNG+ +++ +L L E+ S + L + L ++ S + V +++++ KA+VRIHLF LL EE K+ CA+ +EE +V + LL+ VL K G TPKWM PLLL +DLYEK+ LG RRA++ V S WKW+DI SG+WC YT A+NK IDDAFW+G+ SVR+T GRR+Y +QF +MIQ NEETGNRRP+M+S+K K V A + N AM++ E EK+ S ++ GL D+ S L+KACVG + IP D D L+A +RLCLRLT+ ++ A +FA LGGVK LL L+QSS FSG +LATLL+RH++E+P+TL HTMEKV+R T S S KE ++++RVLAPAACR IF V K LR + S NKRG+ +ED RLL+K+LP + P P L V++ ++CDLL+ L P E+ A V + +IRN + DL V A+ D+S S++M ++P L KSA+CRLLAELV+SY GCA+L+ +H++ + E + E+TSALAFLLD LL S DKD LV+TL+A+LASCNH PEAQS LV EVK ALNR+L PES KH+++Q+LT L+STMIESCP++ Q Q AFKQ MNN+VK MLKRGL+ DLAR+PH LD+SSP +A T+N+ALKPLE LSRIVN P A + R + P S E SS LL +G +T S+ TRAQ E S DAE TE DVS ES+ D ES +ED D+ +++ L +E D S D+ + +D H+ +GH DD + + D EEETE+
Sbjct: 1355 LQALMDMGFPRDQCVEALLFTTSLEQATDYLLSTPPSLLRAPHGAVAAAAAAAVSTAMDTDGNDEDAVMRAIALSLTAD------------SDRADDAGVVEKEPQEHVLQGEPLSKQVLDQFVQQALGGCLSLLDSLPETVYRVCSLVVAMVNRNGEMYRDSMLASLAKEIGSCVTSLQEHLDGVDSLGSKADA-VIQSSDAMKASVRIHLFTLLCEEMKIACARQLEEKDLVLKLIRLLSSGQQVLAVRAKVNDGNECKPADGVGTTPKWMAPLLLCLDLYEKMSLGTRRRAAMEKVTSHMWKWFDIVSGKWCPYTAANNKIIDDAFWAGQNSVRVTAGRRRYLLQFSSMIQVNEETGNRRPMMLSLKEKPKEPTV--DEANRMDENAAMDVDETEKESSKGSQ---------LSGLNLDQGSELIKACVGLLCIPADPDTLHAAMRLCLRLTRHYQHAEKFASLGGVKQLLNLSQSSTFSGFLTLATLLLRHLVEEPETLHHTMEKVLRAYTGPSAAPSTKEFHFLMRVLAPAACRDVQIFSEVCKTFLRADLSLTNKRGD-EEDNRLLMKALPPR-PGCNLPQLSGVAREIVCDLLDALTIPVPPEETITTPPPATETDGTAPVAIPPARRYAGRNVRQQELIRNSSSNDLLNHEAEETVDDARRDLLARKKDSS-SEEMDRRRRPLLPKSAICRLLAELVRSYAGCARLVAEHIFPAKTSELVSEETSALAFLLDNLLPSCQTAGDKDSPALVRTLVASLASCNHAPEAQSCLVAEVKGALNRALTMPESNDKHNRLQSLTALLSTMIESCPATGLQPPQALTTAFKQQ-NLGMNNMVKFMLKRGLVNDLARIPHCLDMSSPLMAFTVNAALKPLEALSRIVNLPPAAPVAGRPATK--PKSGDEGEQQE------SSALL-----------QGNST--SDNTRAQAVEISSALDAENTEQDVSAAGESLENLADNDGGGESGDNNLEDIMDQHSQALVEALIVAAENPSQMETDDSAHDSQMRTHNESEFIGADDAHYHGNDPRDSSDSDSQSESGHSDDDDDEADDDDDDDDDGGEEETEE 2393 HSP 3 Score: 384.03 bits (985), Expect = 1.500e-105 Identity = 175/254 (68.90%), Postives = 202/254 (79.53%), Query Frame = 0
Query: 21 KYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSI-GNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLS 273
K P HQ+K +KPLL +SRLGRALAELF LLVKLCVGSPIR RR PPTP PS A AVA AL +LL SGL+W PP TSP P FRLTF CSVGFT PMLFD+KK PYHLML KFV+ GG AFFD FYWALS GG++SP++GLE ++LP+GTGEFLD+WLMLLE+MVNPK +L+SPH LPN ++ G+ PF P+KYLI TH+ AF AVM++WGK PLKVYG RM ESVL+ILCHILKGE II EKL+
Sbjct: 1076 KLTPAQQHQMKMVKPLLSTASRLGRALAELFGLLVKLCVGSPIRHRRAHQAPPTPLIPSPSAQAVAGALTQLLTSGLAWEPPVTSPTPKFRLTFLICSVGFTSPMLFDEKKLPYHLMLHKFVSCGGQSAFFDAFYWALSAGGRVSPDQGLEYSDLPEGTGEFLDAWLMLLEKMVNPKNILDSPHQLPNKALHGSKHPFSPLKYLISTHRQAFDAVMRLWGKRPLKVYGARMTESVLTILCHILKGEVIIQEKLA 1329 HSP 4 Score: 321.242 bits (822), Expect = 1.661e-86 Identity = 176/300 (58.67%), Postives = 221/300 (73.67%), Query Frame = 0
Query: 1879 PSTSGTS-QNP-DYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSN------VPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFF----NNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
P+ +G+S Q P +Y S+LG++EIPEGVDPSFLAALPE+MRQEVI +Q RLQ +RQRA+ Q T+ + V EV+ EFLAALPPNIQEEVLAQQR+EQQR + NP PVDP +F TLP +LRQS+LADMEDSQMA LP +LAAEAQ+LRRE E RNR M +RFF N+G N+LSSI+R S+ RLG++Y + + R + G AS ++ AN +R +GR LLD+E LSCLL+LLF+D+ K+NTTRLHR+LRNLCYH PTRDWVV+ L+SILER+NE +
Sbjct: 2995 PAAAGSSVQQPSEYQSLLGNIEIPEGVDPSFLAALPEDMRQEVIAEQLRLQRLRQRARAQTTDTTTQVYKQAVLEVSAEFLAALPPNIQEEVLAQQRLEQQRTVVPANPNEPVDPADFLSTLPPALRQSILADMEDSQMAVLPPDLAAEAQNLRREWEARNRQLMADRFFTHVGNSGANALSSIIRNSMGRLGSRYGVHPVHNRGHWGGWGRPVAGASGSSAVV----AQAAANGVRLRGRHLLDHEALSCLLVLLFIDEPKLNTTRLHRVLRNLCYHGPTRDWVVKSLLSILERSNEAR 3290
BLAST of EMLSAG00000004991 vs. Select Arthropod Genomes
Match: XP_016768793.1 (PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase HUWE1 [Apis mellifera]) HSP 1 Score: 954.51 bits (2466), Expect = 0.000e+0 Identity = 491/806 (60.92%), Postives = 582/806 (72.21%), Query Frame = 0
Query: 2193 SASSQQQQQXKYPVIRRNSLLTDKLLRLLSLISIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNFEHDR----------SGKDKGGLLDRFTKETVVLNAPSK-QXKKDMQEAKKSSPPSDTPAESTTTNTTTAPA------------STTTSTSDTTTIADQTNEEN-----KMDVDENSSDSTSEAKANSQW----KIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQ--EQLAHIQEQLADNKEMESSSTASSCS-------SNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
SA Q + VI+R+S LTDK+L+LLSLISIG + + + + + ++ P +HL+LAV VLTSKSCSEEGLED TALLLNLSH P PTR +IL+LLL+GA EL NVV ++ DL L+ +K N E R +G + LLDRFT E+VV+ APSK + D+Q ++ S T +++ T+T +S+++ + TN+ + K+ + N + + Q+ K+ LSE L LD LWE L CL +L TPD HAVLVLQ +VEAFFLVH++S+ ++ E R+ L + +DN+ S + S +L PD KFL FAETHRTVLNQILRQ+T HLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFE+SFREL RR+ +E KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF SPGD+VTYMI SS NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHILG VK+TDMESEDY+FYKGL +L H ++DLGY+LTFSTE+ EFGV ++RDLIPNGRNI VTEETKL+YIRLVCQMKMTGAIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLP +DI+DLK+NTEYHKY + I WFWRALR FDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLRT LLKAIHECSE F
Sbjct: 3293 SAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYRRQEGNKNKRTDVNKEQSIAAP--EEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDL---LMELKVLNRELRRNSIEEQPSTSTGGSRWVLLDRFTNESVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRILKVIVQIREAVRLANNKKTSTQSSESSVDTENTNQASESGNLKLYIFINIAXFSQLNNICIQFIGEDKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTSTENREVVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIIVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNXXXIXIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGF 4093 HSP 2 Score: 301.982 bits (772), Expect = 1.250e-80 Identity = 143/262 (54.58%), Postives = 180/262 (68.70%), Query Frame = 0
Query: 28 HQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSIG---NFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKGEDDKNSGG 286
H++ K LL +S++GRALAELFA+LVKLC+GSPIRQRRGQ +PP PA PSQ + +VA+ALN +L GLSW P S +P ++TF C++ F P LFD+K YP+HLML KFV GG AFF F+W LS GG+ EGLE LPD TGEFLD WLM+LE+MVNPK +L+SP+ + + G ++ FDP+KYL HK AF AVM +WGK PLK YG M E +LSIL HIL+ E+II E K ++ N GG
Sbjct: 1118 HRLTYPKHLLGLTSKMGRALAELFAILVKLCMGSPIRQRRGQQMPPIPALPSQASRSVATALNCVLIRGLSWDKLPPSTVPKLQVTFLMCTLSFVSPALFDEKGYPFHLMLQKFVKLGGQNAFFAAFHWVLSGGGQFPLSEGLEHPNLPDCTGEFLDGWLMILEKMVNPKTLLDSPYVVSSKCTGIYKVYETFDPIKYLTDIHKEAFEAVMLMWGKKPLKNYGVHMTELILSILRHILRSEQIIKEHAEK-----EENNEGG 1374 HSP 3 Score: 283.878 bits (725), Expect = 3.401e-75 Identity = 157/278 (56.47%), Postives = 192/278 (69.06%), Query Frame = 0
Query: 1899 IPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVP-EVNPEFLAALPPNIQEEVLAQQRMEQQRQLS-QVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNN---GGNSLSSILRGSVSRLG--NQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERAN-ETKDI 2168
+P+GVDPSFLAALPE+MR EVI +Q RLQ IRQRAQ E P EVNPEFLAALPP IQEEVLAQQR+EQQR + NPE PVD FFQ L SLRQ++L DME+SQ++ LP +LA EAQ+LRRE E RNR+ M +RF N+ G +LSSILR S G +Y I + R G + +IT H A +R +GRQLLD+E ++C+L+LLFVD+ KINT RLHR++RNLCYH TR+WVVR L+SI+ER+N E KDI
Sbjct: 2926 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQGNAALSSILRNSGRGRGGGTRYAIHTVAQR------GWQPWSNGDPSITH---HGA----GLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSNDENKDI 3190
BLAST of EMLSAG00000004991 vs. Select Arthropod Genomes
Match: gb|EFA10984.1| (E3 ubiquitin-protein ligase HUWE1-like Protein [Tribolium castaneum]) HSP 1 Score: 912.138 bits (2356), Expect = 0.000e+0 Identity = 472/774 (60.98%), Postives = 563/774 (72.74%), Query Frame = 0
Query: 2205 PVIRRNSLLTDKLLRLLSLISIGRPDSLNNKD-DKKGTGSKEESGSGNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSI-----KSTNFEHDRSGKDKGGLLDRFTKETVVLNAPSKQXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEENKMDVDENSSDSTSEAKANSQWKIESLSEQLS-LDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKK------VQQKETRQEQLAHI--------QEQLADNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
PV+ RN+ LTD LLRLLS+I+ G P+ K T +K + + P +A HL AV+V+T K+CSEEGLE +T LLLNL+ + +IL LLL GA +G +VR + ++ EL K TN + KG + DRFTKETV++ A +K ++ + + PS P T+ +S+ + Q K +V A A+ + I +LSEQLS +DSLWE L CL +L TPDHHAVLVLQ +VEAFFLVH+ P +K++ Q E+R A E N E E + S+ S ++ P+ +KFL+FAE HR VLNQILRQST+HLADGPF+VLVDHTR+LDFDIKRRYFR+ELERMD+ +RRE+ AVHVRR N+FE+SFREL RR+PEE KNRFYIVFE EEGQDAGGLLREWYVIISR+IFNPMYALF SPGD+VTYMI +S NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHILG PVKYTDMESEDY+FY+GL +L+ + +++LG DLTFSTEI EFGVTE RDLI NGR++ VTEETK++YIRL CQMKMTGAI++QL+AFL+GFY+IIP RLISIFNEQELELL+SGLP +DIDDLK+NTEYHKYQ NSLQI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGFA LEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLP YETYDKLR+YLLKAIHECSE F
Sbjct: 3268 PVVNRNTQLTDNLLRLLSVITSGMPELAKPPTVPIKATKNKLKENTT-----PTNALHL--AVNVITYKNCSEEGLEFITNLLLNLASSTLEMSYMILNLLLSGAVRIGEIVREQINNMLIELKEYNAQKRKVTNNDELGPSTSKGTIADRFTKETVIITASTK-----VKASCELQLPSMVP--------LTSKSSSQLFFLRVLRVIVQIRNSFKQNV--------KRADASWCYDIPALSEQLSSIDSLWETLSQCLSELEHTPDHHAVLVLQPAVEAFFLVHSPQQGPSKKQQDTEPSSSQAPESRTTTSAESGDLPPAPSTENKFVNPETELHTAEST--SKTVPPEQQKFLSFAEKHRRVLNQILRQSTSHLADGPFAVLVDHTRILDFDIKRRYFRSELERMDQGIRREETAVHVRRSNIFEDSFRELYRRTPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISRDIFNPMYALFTVSPGDRVTYMINSASHYNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGIPVKYTDMESEDYSFYRGLVYLMENNINNLGLDLTFSTEINEFGVTETRDLIVNGRHVPVTEETKMEYIRLSCQMKMTGAIKQQLNAFLDGFYDIIPMRLISIFNEQELELLISGLPNVDIDDLKANTEYHKYQANSLQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPVYETYDKLRSYLLKAIHECSEGFGF 4011 HSP 2 Score: 546.969 bits (1408), Expect = 3.445e-155 Identity = 426/1325 (32.15%), Postives = 659/1325 (49.74%), Query Frame = 0
Query: 392 LQILRDMGFSRERCLEAMDRTGAMYEATEYLLTG-------TQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEV-KSAIDVLLDKLLELEKSQSPIEEDVFRTTESNKAAVRIHLFILLFE-----ECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCSGSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIKSKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPNEEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNS-QTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEV-SQSVICDLLNFLVKTPDICESSNNATVVESAT-ELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKDMKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSK---ADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQ-QPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPT-ALVSSARKSKNDVPDSSAEDSTSNNPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERDGSDNFTIIPQVGSNVNSDQD----------HHQDG-----SRFAGHGCQDDVDRAXESSQXD--------------------------AAIEEETEDLIEEEEDDDGEEEDDEVNGGSGVSTDGGA----------NSANHVTP-SHPLLMGRQNLETGTGNARNS-ASTRSLPRTRGNSLRPYFQLNSRAQNNPSTPAILQNFLGGSNPQELITFG---------FRRSGAPLMMDVVGLSLLDTMDNDMPDLDSAT---LGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTN---------LKPEIIEVIE--NTRDEVRRKKEEERKIIK 1619
L+ L DMGFSRE +A+ T + +AT+YLLT +QS N++ E + +M+AIA+SL V S+N +++ Q GK L++S++DEFT AL CL L+D PE V + C+LL+ + RNG+ F++ +L L+ E+ ++A +V L+ + + P V + + + A +HL+IL FE E K+ CA V + ++ ++ L+ ++ V+ R S V PKW+ L ID KV +R+ ++ +R W+W+ + +G+W Y+ ++NK I+DA+W+GE SVR+ GRR+YT+ F M+Q N+E+GN RPI +++ + G SS S+ + EE + +ED L +++ +++ACV + + ++ D L+++L++C+RLT++FE A F + GGVK L+ + Q F LAT+L+RH LE+P+TL + +EK+IR TL++ KE+ Y+ R + A RSP F VAK+ILR + + +N+ D D+R+ VK+ P + APPL EV S VICDLLN L+ ++ + + T ++A E + K + TGD P + + MKK L KS + ++L + V SYG A++IT++ Y++G E IKED +ALAF+LD +L +D D + + LIAA+AS NH PEAQ LV EV+ AL R+L+ PES KHS++Q + +IS MI++CPSS + + QP Q MNNIV++M+++ L DLARVPH LDLSSPN T+N+ LK LE LSRIVNQP A V +RK++ +S+ T SG+S T TN++ D V D+E TEHD++ +I+ + + N+ M+DQLLE+ G+DN V S+ N D H +D S +G + D+ + D A+ + +D++ + + G E + + D A NS++H P HPLL+ R T N NS +TR+ TR R + N R NP+ P ILQ LG PQ + R S ++MD GL L + + + +D ++ LG A L+ I L WN ES+ +D ES++D T +K +E++E + D +RR K E IK
Sbjct: 1357 LRQLMDMGFSREHVTDALLNTLTVEQATDYLLTNPPSLYRISQSFMNSD--------------------EDDQMMQAIAISLGV-------SNNLPSVSKKRQIQDDGKP----------LAESLIDEFTNHALEACLRLLDNTPECVHKICELLVTIMKRNGRTFRDNLLDTLVKEIYENAKNVCY--ALKQPECELP---SVAKLPCATRLANYLHLYILFFEVSSYFEMKIPCALAVHKIDLINLLIKLICMAEKVMSR-SEKVTEPKWLASSFLLIDAASKVATCAHRKRNMHLSTTRVWRWFHLETGKWTPYSNSNNKLINDAYWNGEQSVRVICGRRRYTVTFSNMLQLNDESGNNRPICMTLINSG----------LSSCSDVFIVNLEESLRAELTEKED--KRCQYVRNLDKAQETKIIRACVKLMHLTIEKDLLHSILQICIRLTRDFELAKIFVQEGGVKCLIAMKQVREFPSFGILATILIRHTLEEPNTLSYAIEKIIRARTLSTIPPYYKELLYLTRQIGGAVTRSPETFFEVAKNILRVD-TDVNRE---DVDSRIPVKTEPPT--RTRAPPLEEVVSNEVICDLLNALLNPLEVVDDTQPQTSPQNAPPETSSTKPSES-------VTGDNNDSGSETPAQAEANADDQSEPIQSMKKMMLPKSVILKILGDAVVSYGPVARIITEYTYKAGSNECIKEDCTALAFILDYILPEADDLSDSDCATMCRMLIAAIASSNHSPEAQFTLVSEVRAALLRALVMPESTEKHSQVQLIAGIISIMIDNCPSSQARVKSQPPTPQ-----MNNIVRLMIRKSLFNDLARVPHFLDLSSPNTPFTVNACLKSLEALSRIVNQPVPANVQKSRKNQQATDESAG--------TQSGTS----------------TEGTNAQREVVIDDSVVIEDSENTEHDIAVVTSTIEVEA--------NENENVLVHMIDQLLEQ-GNDNNQSFSDVASSRNHTMDIDEDGNLTFEHERDATEELMSTDSGESDSNPSDQVEDEENDDNEGDDENIDDADDTNYDDDDGSDRRRASYMDRNDDVLMIQYTNPGAENEVVTRMVAYPMGDHAAFPMPLFDEINNSSDHTGPIVHPLLLSR------TANESNSNTATRTQRVTRAR--RYQYLFNPR---NPNPPVILQRLLGPHEPQVTLASANSILGGPPEMRESARLVVMDNFGL--LPSHEEQIDFVDQSSGYLLGPSLAATLNHISPVLHWWNIESKCLDYESVYDVTTEVCNKLIPILIKQRNVEIVEKRKSHDTLRRFKFFEDDYIK 2562 HSP 3 Score: 315.849 bits (808), Expect = 6.710e-85 Identity = 151/247 (61.13%), Postives = 181/247 (73.28%), Query Frame = 0
Query: 30 IKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNL----SIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKL 272
+K IKPLL ASSRLGRALAELF LLVKLCVGSPIRQRRGQ++ P P+ A +VA+ALN LLA GL + P P+ R+TF CSVGFT PMLFD+K+YPYHLML KFV GG FF+TF WALS GG + E+ E LP+GT FLD+WLMLLE+MVNPKA+LESPH + N S FDP+KYLI+ HKLAF AV+ +W K PL YG R++E++L++L HIL+GEKII EKL
Sbjct: 1080 LKYIKPLLGASSRLGRALAELFGLLVKLCVGSPIRQRRGQNIIAAPPLPTPYARSVATALNVLLADGLDYERLPACPLIKSRITFLICSVGFTSPMLFDEKRYPYHLMLKKFVALGGQATFFNTFRWALSAGGTIPIEKAFEDGPLPEGTAGFLDAWLMLLEKMVNPKAILESPHIISNRSSQSSTYRGYNFDPLKYLIQIHKLAFEAVILLWNKAPLTTYGTRLSENILTVLRHILRGEKIIKEKL 1326 HSP 4 Score: 246.128 bits (627), Expect = 1.121e-63 Identity = 137/279 (49.10%), Postives = 178/279 (63.80%), Query Frame = 0
Query: 1898 EIPEGVDPSFLAALPEEMRQEVIDDQRRL---QNIRQRAQQQNTEGSNVPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQV-NPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANS------IRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETK 2166
EIP+GVDPSFL ALP +MRQEV++ R L Q I R + EV+PEFLAALPP++QEEVL+QQR+EQQRQ + NP PVD G FF+TL SLR +L+DMEDSQM+ALP +LAAEAQ+LRR+ E RNR + ER + +L+SI+R S ++ S F A RE F+ NS ++ +GRQLLD+EGLSCLL+LLF DD + RLHR++RNLCYHAPTR+W+V L+SI++++ K
Sbjct: 2829 EIPDGVDPSFLEALPPDMRQEVLEQHRILRLQQRIAARTENPEAAAGTTTEVSPEFLAALPPSLQEEVLSQQRLEQQRQAAAQANPNEPVDAGAFFETLQPSLRTMILSDMEDSQMSALPPDLAAEAQTLRRDWEARNRQLVQERILQS---NLTSIIRHSAR-----------ARQQPLHHSSGHFHRAQWTHWNRE-----FMNNSATPTPPLKIRGRQLLDHEGLSCLLVLLFTDDPHLGKLRLHRVIRNLCYHAPTREWIVNALLSIIDKSVHVK 3088
BLAST of EMLSAG00000004991 vs. Select Arthropod Genomes
Match: gb|EEC02702.1| (E3 ubiquitin protein ligase (URE-B1), putative, partial [Ixodes scapularis]) HSP 1 Score: 883.248 bits (2281), Expect = 0.000e+0 Identity = 466/795 (58.62%), Postives = 552/795 (69.43%), Query Frame = 0
Query: 2261 HLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKST----------NFEHDRSGK----------DKGGLLDRFTKETVVLNAP---------------------SKQXKK-----------DMQEAKKSSPPSDTPAESTTTNTTTA---PASTTTSTSDTTT--------IADQTNEENKMDVDENSSD-------STSEAKANS-----QWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAA-------------STQPDEKKKVQQKETRQEQLAHIQEQLA----------DNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
HL+LAV+ LTSK+CSEEGLED T+LLL LS G R ++L+LLL+GA++LG V A++ L EL ++ + N D +G+ G + DRFT TVV+ AP SK + ++EA +S + S TA P + +TST + A Q+N N MDVD S++ +A S + LSE+L LD+LW L DCL +LAET D HAVLVLQ +VEAFFLVHA+ S++ +++ + E+R+ QLAHI +++A N + + + S L PD +KFL FAETHRTVLNQILRQS+ LADGPF+VLVDHTRVLDFD+KRRYFR ELER+DE RREDLAVHVRRE+VFE+SFREL RR PEE KNRFYIVFEGEEGQDAGGLLREWY IISREIFNPMYALF TSPGD+VTYMI +S N NHL YFKFVGRVIAKA+YDNKLLECYFTRSFYKHILGKPVKYTDMESEDY+FY+GL FL+ H V LGY+LTFS E+QEFGVTE+RDL P GR++ VTEET +Y+RLVCQ KMTGAIR+QL+AFLEGFY IIP+RLI+IFNEQELELL+SGLP+ID+DDL+++TEYHKYQ NSLQI WFWRALRS DQ D+AKF+QFVTG+SKVPLQGF LEGMNGVQ+FQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRT LLKAI EC+E F
Sbjct: 405 HLKLAVETLTSKACSEEGLEDATSLLLRLSRGCPSARQVVLRLLLEGARDLGATVGASIGALLAELSTLNARLASERLALEENERDDAAGRLPSPAPGSRVAAGTIQDRFTHSTVVITAPGAPLPAGAGRELQLPSMAALTSKMSSQAFFLRVLKVVIQLREAAQSHYQARRRRLSGACELPTASAEPLALSTSTGSMASTPRLENAPCAVQSNA-NHMDVDGAGGSQHMLPVMSSTPTRAGSLPPDAADEPPPLSEELRLDALWSTLSDCLLELAETQDQHAVLVLQPAVEAFFLVHASVPPGSTSVPGISGSSREAARRREYRSESRETQLAHIHQEMAPLSPLPLQDNQNAQASGGAAGGAQGSEFLQPDVQKFLGFAETHRTVLNQILRQSSTPLADGPFAVLVDHTRVLDFDVKRRYFRHELERLDEGSRREDLAVHVRREHVFEDSFRELHRRPPEEWKNRFYIVFEGEEGQDAGGLLREWYTIISREIFNPMYALFTTSPGDRVTYMINPASHCNSNHLSYFKFVGRVIAKAVYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYSFYQGLVFLLEHGVRALGYELTFSVEVQEFGVTEVRDLKPGGRHLPVTEETTQEYVRLVCQEKMTGAIRRQLNAFLEGFYEIIPKRLIAIFNEQELELLISGLPSIDVDDLRAHTEYHKYQPNSLQIQWFWRALRSLDQADRAKFLQFVTGTSKVPLQGFVALEGMNGVQRFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTMLLKAIQECTEGFGF 1198 HSP 2 Score: 105.916 bits (263), Expect = 1.773e-21 Identity = 48/91 (52.75%), Postives = 65/91 (71.43%), Query Frame = 0
Query: 2105 KGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNKSKRSSTATPVKSAS 2195
+GRQLLD+E L+CLL+LLFVD+ ++NT RLHR+LRNLCYH PTR WVV+ L+SILE+ E K + + Q+ P+ + + T V S S
Sbjct: 12 RGRQLLDHEALACLLVLLFVDEPRLNTGRLHRVLRNLCYHPPTRHWVVKSLLSILEKTKENKPLEGNSQAVADPPHHRGKKWSKTHVGSFS 102
BLAST of EMLSAG00000004991 vs. Select Arthropod Genomes
Match: AHN59762.1 (CG8184, isoform F [Drosophila melanogaster]) HSP 1 Score: 759.985 bits (1961), Expect = 0.000e+0 Identity = 365/565 (64.60%), Postives = 422/565 (74.69%), Query Frame = 0
Query: 2439 SLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKK------------------------KVQQKETRQEQLAHIQE----------QLADNKEMESSST------------ASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+LS+ L LD LW L +CL L E+ D AVLVLQ +VEAFFL+HA+ +K + QE + + D M+ +ST A S L D +KFL FAE HRTVLNQILRQS HL+DGPF+VLVDHTR+LDFD+KR+YF+TELER+DE +RRE+ V VRR VFE+SFR L R PEE KNRFYIVFE EEGQDAGGLLREWYVIISREIFNPMYALF SPGD+VTYMI SS NPNHL YFKFVGRVIAKA++DNKLLECYFTRSFYKHILGK VK+TDMES+DY FYKGL++L+ + +S LGY+LTFSTE+QEFGVT+IRDL PNGR+ VTEE K +Y++LVCQ+KM+G+IR+QL AFLEGFY+IIP+ LISIFNEQELELL+SGLP IDI+DLK+NTEYHKY S QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF LEGMNG+QKFQIHRDDRSTDRLP AHTCFNQLDLP Y++YDKLR+ LLKAIHECSE F
Sbjct: 4562 TLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERLDEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFGF 5126 HSP 2 Score: 250.751 bits (639), Expect = 3.847e-65 Identity = 157/399 (39.35%), Postives = 217/399 (54.39%), Query Frame = 0
Query: 1793 LTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQPRESEEAIGGXSXIASSNPIPSTSG--------TSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSN--VPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAF----------------LANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANE 2164
L +++PG + S +P VA+ S S T S +S + +P + +A G A++ ST+G T +P+ + LGD+E+PEGVDPSFLAALP EMR+EVI + R+Q IRQRAQQ + ++ + EVNPEFLAALP NIQ EVL QQR+EQQRQ +Q NPE PVD FFQ LP +LRQ++L DME+SQ+A+LP ELAAEAQ LRR+ E RN M +N + + L+ + R S + +AS A + +H LA + LLD + L+ LL+ LFV+D K+N+ RLHR++RNLC+H PTRDW++ L+SI+++ NE
Sbjct: 3533 LVHAEDDLWPGTVVSAGTREESSAPAVATESTEAVNESNQPEPTPESSESTSPNPQAPSAEPTPLEPGAAVDASVGAQTPATTEEAASTAGASGTTATITDMSPEVRAALGDLEVPEGVDPSFLAALPSEMREEVIQEHLRMQRIRQRAQQNAIQIAHDSLVEVNPEFLAALPLNIQSEVLMQQRIEQQRQAAQTANPEDPVDTAAFFQNLPENLRQAILTDMEESQIASLPPELAAEAQFLRRDWESRNGARMDAHPPSNALSRFQTTLQ----------------SLDDTRWHSSIWYDASGNAQPQHHQHTTIVHQHPHAHHAPNMGKPLALLMMEDENILLDPDSLATLLLFLFVEDQKVNSMRLHRVIRNLCHHEPTRDWIISALISIIQKTNE 3915 HSP 3 Score: 228.794 bits (582), Expect = 1.813e-58 Identity = 124/269 (46.10%), Postives = 168/269 (62.45%), Query Frame = 0
Query: 21 KYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSP-IRQRR-GQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGG---------KLSPEEGLESNE-----LPDGTGEFLDSWLMLLERMVNPKAVLESPHTL-PNLS-IGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEK 271
KY P Q + IK LL ASSRLGRALAEL LVKL VGSP RQRR + P+ A +A L+ +L +G S P+P +LTF CSVGFT PMLFDDK+ +HLM+ +F GLKAFF+ FYWALS S E+ ++S+E P+GTGEFLD+WL LLE+MVNP+A+L+SP+T+ P L+ + + PF+PV YLI H+LA A+ +IW + P+ YGP M E+++ IL H++K + + ++
Sbjct: 1180 KYTP--EEQFRYIKSLLAASSRLGRALAELLGTLVKLSVGSPQTRQRRINDLISNITKVPTPEAREIARILSSILVNGFSHESILPKPVPKLKLTFLICSVGFTGPMLFDDKRSAFHLMISQFCAENGLKAFFEMFYWALSLDNVSQKFEFDQWASMEDLMQSHEDDKRDCPEGTGEFLDAWLQLLEKMVNPRAMLDSPYTMSPRLNYMPDSVPFEPVFYLIHIHQLAMQALRRIWARRPIPNYGPSMFETMILILKHLIKSHQTLLDR 1446 HSP 4 Score: 115.161 bits (287), Expect = 4.898e-24 Identity = 89/378 (23.54%), Postives = 169/378 (44.71%), Query Frame = 0
Query: 386 GFSFYDLQILRDMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKS-VMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEK---SQSPIEEDVFRTTESNKAAVRIHLFILLFEE-----CKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCSGS------VGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK 748
G + ++ L DMGF +EA+ ++ EAT+YLL ++SS + + + A M +DVDV + E + S + L S + D F +A+ ++ +P+TV DL+ ++ R+ K+ + + + +D L +L E K S++ E+ + + VR+ LL EE + L I + ++V ++ L +S +L++ + + P+W+ L+ ID + + + R+A++ +V S W+WYD S+G+W Y+ A+N+ I +A+ +GE + I GR++ T+ M Q +E +G RP+ ++K
Sbjct: 1467 GLNADHIKTLTDMGFMHYHVIEALRTNASLEEATDYLLNNPEASSLSTTGGQSSSSAPPPPPPP----------SASTMDIDVDV---------PADGESTQSKSSTSPNSSYDYKHLKLMPSLIFDRFCDEAISRAFEFMEAIPDTVNSAADLIAVLYRRHNHLNKQVFVTNFVRNIIQWVDFTL-QLFEPAKTTGSKASRLEECLTGMTATRLFVRLKTSTLLLEENYSDLHRPLVEAITAQDAMVSLVKLLDCMSQWMLEQSTETNTGQQRATVPRWVQNLVDLIDAIDSISHILQRKANMRAVSSDVWRWYDASTGKWNAYSEANNELIRNAYAAGERWLYINIGRQRCTVSLNCMTQVSEASGTHRPVCPALK 1824 HSP 5 Score: 90.1225 bits (222), Expect = 1.681e-16 Identity = 50/141 (35.46%), Postives = 83/141 (58.87%), Query Frame = 0
Query: 824 AVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVI--RTSTLNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVA 962
++LRLC RLT+ ++ A F R GGV++LL L Q+ F G + A +++RHVLE P L+ ME+V+ R + + +++ Y+L ++ A R+P IFV+ AK++L+ E +D R++VKS + P + A
Sbjct: 1984 SLLRLCARLTRNYDNAQVFIRRGGVRMLLHLQQACSFMGFPTYANIILRHVLEAPPVLQEAMERVLGMRAAGI-VPVGHRDLIYVLTQVSSAVSRNPQIFVAAAKEMLKGEYLINTNSTTLADDARVMVKSKFGQQPTAAA 2123 HSP 6 Score: 88.1965 bits (217), Expect = 6.587e-16 Identity = 58/208 (27.88%), Postives = 105/208 (50.48%), Query Frame = 0
Query: 1049 KPFLSKSAVCRLLAELVKSYGGCA-KLITDHVY---ESGIVENIKEDTSALAFLLDELLTSKADK---DIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVS 1249
KP +S++ + +LLA+ ++Y +++ HVY +S ++ ++ + L LLD L ++ + + LI +L+ C P Q + E+ A+ R+L QP+S KH+++Q L L +IES P + NN+ +++L++G++T L +V DLS+ N T+ + L+P+E L R T L S
Sbjct: 2257 KPTISRATLLKLLADSTRAYQSLVPRVLLSHVYTPADSPLISAPQQ--TFLGVLLDRFLPITQRHHVPEVSTMTRVLICSLSDCYQQPGVQVHVAEELHAAVARALAQPDSAEKHTRLQVLMSLFPNLIES----------PMLYDKVHMHRNNMYRVLLRQGVMTYLTKVAQYKDLSNHNTLSTLAAILRPMEILLRFSVSTTTLGS 2452
BLAST of EMLSAG00000004991 vs. Select Arthropod Genomes
Match: AHN59760.1 (CG8184, isoform D [Drosophila melanogaster]) HSP 1 Score: 759.985 bits (1961), Expect = 0.000e+0 Identity = 365/565 (64.60%), Postives = 422/565 (74.69%), Query Frame = 0
Query: 2439 SLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKK------------------------KVQQKETRQEQLAHIQE----------QLADNKEMESSST------------ASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+LS+ L LD LW L +CL L E+ D AVLVLQ +VEAFFL+HA+ +K + QE + + D M+ +ST A S L D +KFL FAE HRTVLNQILRQS HL+DGPF+VLVDHTR+LDFD+KR+YF+TELER+DE +RRE+ V VRR VFE+SFR L R PEE KNRFYIVFE EEGQDAGGLLREWYVIISREIFNPMYALF SPGD+VTYMI SS NPNHL YFKFVGRVIAKA++DNKLLECYFTRSFYKHILGK VK+TDMES+DY FYKGL++L+ + +S LGY+LTFSTE+QEFGVT+IRDL PNGR+ VTEE K +Y++LVCQ+KM+G+IR+QL AFLEGFY+IIP+ LISIFNEQELELL+SGLP IDI+DLK+NTEYHKY S QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF LEGMNG+QKFQIHRDDRSTDRLP AHTCFNQLDLP Y++YDKLR+ LLKAIHECSE F
Sbjct: 4585 TLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERLDEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFGF 5149 HSP 2 Score: 251.136 bits (640), Expect = 3.725e-65 Identity = 157/399 (39.35%), Postives = 217/399 (54.39%), Query Frame = 0
Query: 1793 LTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQPRESEEAIGGXSXIASSNPIPSTSG--------TSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSN--VPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAF----------------LANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANE 2164
L +++PG + S +P VA+ S S T S +S + +P + +A G A++ ST+G T +P+ + LGD+E+PEGVDPSFLAALP EMR+EVI + R+Q IRQRAQQ + ++ + EVNPEFLAALP NIQ EVL QQR+EQQRQ +Q NPE PVD FFQ LP +LRQ++L DME+SQ+A+LP ELAAEAQ LRR+ E RN M +N + + L+ + R S + +AS A + +H LA + LLD + L+ LL+ LFV+D K+N+ RLHR++RNLC+H PTRDW++ L+SI+++ NE
Sbjct: 3556 LVHAEDDLWPGTVVSAGTREESSAPAVATESTEAVNESNQPEPTPESSESTSPNPQAPSAEPTPLEPGAAVDASVGAQTPATTEEAASTAGASGTTATITDMSPEVRAALGDLEVPEGVDPSFLAALPSEMREEVIQEHLRMQRIRQRAQQNAIQIAHDSLVEVNPEFLAALPLNIQSEVLMQQRIEQQRQAAQTANPEDPVDTAAFFQNLPENLRQAILTDMEESQIASLPPELAAEAQFLRRDWESRNGARMDAHPPSNALSRFQTTLQ----------------SLDDTRWHSSIWYDASGNAQPQHHQHTTIVHQHPHAHHAPNMGKPLALLMMEDENILLDPDSLATLLLFLFVEDQKVNSMRLHRVIRNLCHHEPTRDWIISALISIIQKTNE 3938 HSP 3 Score: 228.794 bits (582), Expect = 1.669e-58 Identity = 124/269 (46.10%), Postives = 168/269 (62.45%), Query Frame = 0
Query: 21 KYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSP-IRQRR-GQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGG---------KLSPEEGLESNE-----LPDGTGEFLDSWLMLLERMVNPKAVLESPHTL-PNLS-IGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEK 271
KY P Q + IK LL ASSRLGRALAEL LVKL VGSP RQRR + P+ A +A L+ +L +G S P+P +LTF CSVGFT PMLFDDK+ +HLM+ +F GLKAFF+ FYWALS S E+ ++S+E P+GTGEFLD+WL LLE+MVNP+A+L+SP+T+ P L+ + + PF+PV YLI H+LA A+ +IW + P+ YGP M E+++ IL H++K + + ++
Sbjct: 1184 KYTP--EEQFRYIKSLLAASSRLGRALAELLGTLVKLSVGSPQTRQRRINDLISNITKVPTPEAREIARILSSILVNGFSHESILPKPVPKLKLTFLICSVGFTGPMLFDDKRSAFHLMISQFCAENGLKAFFEMFYWALSLDNVSQKFEFDQWASMEDLMQSHEDDKRDCPEGTGEFLDAWLQLLEKMVNPRAMLDSPYTMSPRLNYMPDSVPFEPVFYLIHIHQLAMQALRRIWARRPIPNYGPSMFETMILILKHLIKSHQTLLDR 1450 HSP 4 Score: 115.161 bits (287), Expect = 4.076e-24 Identity = 89/378 (23.54%), Postives = 169/378 (44.71%), Query Frame = 0
Query: 386 GFSFYDLQILRDMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKS-VMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEK---SQSPIEEDVFRTTESNKAAVRIHLFILLFEE-----CKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCSGS------VGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK 748
G + ++ L DMGF +EA+ ++ EAT+YLL ++SS + + + A M +DVDV + E + S + L S + D F +A+ ++ +P+TV DL+ ++ R+ K+ + + + +D L +L E K S++ E+ + + VR+ LL EE + L I + ++V ++ L +S +L++ + + P+W+ L+ ID + + + R+A++ +V S W+WYD S+G+W Y+ A+N+ I +A+ +GE + I GR++ T+ M Q +E +G RP+ ++K
Sbjct: 1471 GLNADHIKTLTDMGFMHYHVIEALRTNASLEEATDYLLNNPEASSLSTTGGQSSSSAPPPPPPP----------SASTMDIDVDV---------PADGESTQSKSSTSPNSSYDYKHLKLMPSLIFDRFCDEAISRAFEFMEAIPDTVNSAADLIAVLYRRHNHLNKQVFVTNFVRNIIQWVDFTL-QLFEPAKTTGSKASRLEECLTGMTATRLFVRLKTSTLLLEENYSDLHRPLVEAITAQDAMVSLVKLLDCMSQWMLEQSTETNTGQQRATVPRWVQNLVDLIDAIDSISHILQRKANMRAVSSDVWRWYDASTGKWNAYSEANNELIRNAYAAGERWLYINIGRQRCTVSLNCMTQVSEASGTHRPVCPALK 1828 HSP 5 Score: 90.1225 bits (222), Expect = 1.586e-16 Identity = 50/141 (35.46%), Postives = 83/141 (58.87%), Query Frame = 0
Query: 824 AVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVI--RTSTLNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVA 962
++LRLC RLT+ ++ A F R GGV++LL L Q+ F G + A +++RHVLE P L+ ME+V+ R + + +++ Y+L ++ A R+P IFV+ AK++L+ E +D R++VKS + P + A
Sbjct: 1988 SLLRLCARLTRNYDNAQVFIRRGGVRMLLHLQQACSFMGFPTYANIILRHVLEAPPVLQEAMERVLGMRAAGI-VPVGHRDLIYVLTQVSSAVSRNPQIFVAAAKEMLKGEYLINTNSTTLADDARVMVKSKFGQQPTAAA 2127 HSP 6 Score: 88.1965 bits (217), Expect = 6.701e-16 Identity = 58/208 (27.88%), Postives = 105/208 (50.48%), Query Frame = 0
Query: 1049 KPFLSKSAVCRLLAELVKSYGGCA-KLITDHVY---ESGIVENIKEDTSALAFLLDELLTSKADK---DIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVS 1249
KP +S++ + +LLA+ ++Y +++ HVY +S ++ ++ + L LLD L ++ + + LI +L+ C P Q + E+ A+ R+L QP+S KH+++Q L L +IES P + NN+ +++L++G++T L +V DLS+ N T+ + L+P+E L R T L S
Sbjct: 2255 KPTISRATLLKLLADSTRAYQSLVPRVLLSHVYTPADSPLISAPQQ--TFLGVLLDRFLPITQRHHVPEVSTMTRVLICSLSDCYQQPGVQVHVAEELHAAVARALAQPDSAEKHTRLQVLMSLFPNLIES----------PMLYDKVHMHRNNMYRVLLRQGVMTYLTKVAQYKDLSNHNTLSTLAAILRPMEILLRFSVSTTTLGS 2450
BLAST of EMLSAG00000004991 vs. Select Arthropod Genomes
Match: AHN59759.1 (CG8184, isoform C [Drosophila melanogaster]) HSP 1 Score: 759.985 bits (1961), Expect = 0.000e+0 Identity = 365/565 (64.60%), Postives = 422/565 (74.69%), Query Frame = 0
Query: 2439 SLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKK------------------------KVQQKETRQEQLAHIQE----------QLADNKEMESSST------------ASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+LS+ L LD LW L +CL L E+ D AVLVLQ +VEAFFL+HA+ +K + QE + + D M+ +ST A S L D +KFL FAE HRTVLNQILRQS HL+DGPF+VLVDHTR+LDFD+KR+YF+TELER+DE +RRE+ V VRR VFE+SFR L R PEE KNRFYIVFE EEGQDAGGLLREWYVIISREIFNPMYALF SPGD+VTYMI SS NPNHL YFKFVGRVIAKA++DNKLLECYFTRSFYKHILGK VK+TDMES+DY FYKGL++L+ + +S LGY+LTFSTE+QEFGVT+IRDL PNGR+ VTEE K +Y++LVCQ+KM+G+IR+QL AFLEGFY+IIP+ LISIFNEQELELL+SGLP IDI+DLK+NTEYHKY S QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF LEGMNG+QKFQIHRDDRSTDRLP AHTCFNQLDLP Y++YDKLR+ LLKAIHECSE F
Sbjct: 4580 TLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERLDEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFGF 5144 HSP 2 Score: 251.136 bits (640), Expect = 3.693e-65 Identity = 157/399 (39.35%), Postives = 217/399 (54.39%), Query Frame = 0
Query: 1793 LTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQPRESEEAIGGXSXIASSNPIPSTSG--------TSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSN--VPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAF----------------LANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANE 2164
L +++PG + S +P VA+ S S T S +S + +P + +A G A++ ST+G T +P+ + LGD+E+PEGVDPSFLAALP EMR+EVI + R+Q IRQRAQQ + ++ + EVNPEFLAALP NIQ EVL QQR+EQQRQ +Q NPE PVD FFQ LP +LRQ++L DME+SQ+A+LP ELAAEAQ LRR+ E RN M +N + + L+ + R S + +AS A + +H LA + LLD + L+ LL+ LFV+D K+N+ RLHR++RNLC+H PTRDW++ L+SI+++ NE
Sbjct: 3551 LVHAEDDLWPGTVVSAGTREESSAPAVATESTEAVNESNQPEPTPESSESTSPNPQAPSAEPTPLEPGAAVDASVGAQTPATTEEAASTAGASGTTATITDMSPEVRAALGDLEVPEGVDPSFLAALPSEMREEVIQEHLRMQRIRQRAQQNAIQIAHDSLVEVNPEFLAALPLNIQSEVLMQQRIEQQRQAAQTANPEDPVDTAAFFQNLPENLRQAILTDMEESQIASLPPELAAEAQFLRRDWESRNGARMDAHPPSNALSRFQTTLQ----------------SLDDTRWHSSIWYDASGNAQPQHHQHTTIVHQHPHAHHAPNMGKPLALLMMEDENILLDPDSLATLLLFLFVEDQKVNSMRLHRVIRNLCHHEPTRDWIISALISIIQKTNE 3933 HSP 3 Score: 228.794 bits (582), Expect = 1.712e-58 Identity = 124/269 (46.10%), Postives = 168/269 (62.45%), Query Frame = 0
Query: 21 KYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSP-IRQRR-GQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGG---------KLSPEEGLESNE-----LPDGTGEFLDSWLMLLERMVNPKAVLESPHTL-PNLS-IGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEK 271
KY P Q + IK LL ASSRLGRALAEL LVKL VGSP RQRR + P+ A +A L+ +L +G S P+P +LTF CSVGFT PMLFDDK+ +HLM+ +F GLKAFF+ FYWALS S E+ ++S+E P+GTGEFLD+WL LLE+MVNP+A+L+SP+T+ P L+ + + PF+PV YLI H+LA A+ +IW + P+ YGP M E+++ IL H++K + + ++
Sbjct: 1179 KYTP--EEQFRYIKSLLAASSRLGRALAELLGTLVKLSVGSPQTRQRRINDLISNITKVPTPEAREIARILSSILVNGFSHESILPKPVPKLKLTFLICSVGFTGPMLFDDKRSAFHLMISQFCAENGLKAFFEMFYWALSLDNVSQKFEFDQWASMEDLMQSHEDDKRDCPEGTGEFLDAWLQLLEKMVNPRAMLDSPYTMSPRLNYMPDSVPFEPVFYLIHIHQLAMQALRRIWARRPIPNYGPSMFETMILILKHLIKSHQTLLDR 1445 HSP 4 Score: 115.161 bits (287), Expect = 4.075e-24 Identity = 89/378 (23.54%), Postives = 169/378 (44.71%), Query Frame = 0
Query: 386 GFSFYDLQILRDMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKS-VMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEK---SQSPIEEDVFRTTESNKAAVRIHLFILLFEE-----CKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCSGS------VGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK 748
G + ++ L DMGF +EA+ ++ EAT+YLL ++SS + + + A M +DVDV + E + S + L S + D F +A+ ++ +P+TV DL+ ++ R+ K+ + + + +D L +L E K S++ E+ + + VR+ LL EE + L I + ++V ++ L +S +L++ + + P+W+ L+ ID + + + R+A++ +V S W+WYD S+G+W Y+ A+N+ I +A+ +GE + I GR++ T+ M Q +E +G RP+ ++K
Sbjct: 1466 GLNADHIKTLTDMGFMHYHVIEALRTNASLEEATDYLLNNPEASSLSTTGGQSSSSAPPPPPPP----------SASTMDIDVDV---------PADGESTQSKSSTSPNSSYDYKHLKLMPSLIFDRFCDEAISRAFEFMEAIPDTVNSAADLIAVLYRRHNHLNKQVFVTNFVRNIIQWVDFTL-QLFEPAKTTGSKASRLEECLTGMTATRLFVRLKTSTLLLEENYSDLHRPLVEAITAQDAMVSLVKLLDCMSQWMLEQSTETNTGQQRATVPRWVQNLVDLIDAIDSISHILQRKANMRAVSSDVWRWYDASTGKWNAYSEANNELIRNAYAAGERWLYINIGRQRCTVSLNCMTQVSEASGTHRPVCPALK 1823 HSP 5 Score: 90.1225 bits (222), Expect = 1.599e-16 Identity = 50/141 (35.46%), Postives = 83/141 (58.87%), Query Frame = 0
Query: 824 AVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVI--RTSTLNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVA 962
++LRLC RLT+ ++ A F R GGV++LL L Q+ F G + A +++RHVLE P L+ ME+V+ R + + +++ Y+L ++ A R+P IFV+ AK++L+ E +D R++VKS + P + A
Sbjct: 1983 SLLRLCARLTRNYDNAQVFIRRGGVRMLLHLQQACSFMGFPTYANIILRHVLEAPPVLQEAMERVLGMRAAGI-VPVGHRDLIYVLTQVSSAVSRNPQIFVAAAKEMLKGEYLINTNSTTLADDARVMVKSKFGQQPTAAA 2122 HSP 6 Score: 88.1965 bits (217), Expect = 6.813e-16 Identity = 58/208 (27.88%), Postives = 105/208 (50.48%), Query Frame = 0
Query: 1049 KPFLSKSAVCRLLAELVKSYGGCA-KLITDHVY---ESGIVENIKEDTSALAFLLDELLTSKADK---DIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVS 1249
KP +S++ + +LLA+ ++Y +++ HVY +S ++ ++ + L LLD L ++ + + LI +L+ C P Q + E+ A+ R+L QP+S KH+++Q L L +IES P + NN+ +++L++G++T L +V DLS+ N T+ + L+P+E L R T L S
Sbjct: 2250 KPTISRATLLKLLADSTRAYQSLVPRVLLSHVYTPADSPLISAPQQ--TFLGVLLDRFLPITQRHHVPEVSTMTRVLICSLSDCYQQPGVQVHVAEELHAAVARALAQPDSAEKHTRLQVLMSLFPNLIES----------PMLYDKVHMHRNNMYRVLLRQGVMTYLTKVAQYKDLSNHNTLSTLAAILRPMEILLRFSVSTTTLGS 2445
BLAST of EMLSAG00000004991 vs. Select Arthropod Genomes
Match: AHN59758.1 (CG8184, isoform H [Drosophila melanogaster]) HSP 1 Score: 759.985 bits (1961), Expect = 0.000e+0 Identity = 365/565 (64.60%), Postives = 422/565 (74.69%), Query Frame = 0
Query: 2439 SLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKK------------------------KVQQKETRQEQLAHIQE----------QLADNKEMESSST------------ASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+LS+ L LD LW L +CL L E+ D AVLVLQ +VEAFFL+HA+ +K + QE + + D M+ +ST A S L D +KFL FAE HRTVLNQILRQS HL+DGPF+VLVDHTR+LDFD+KR+YF+TELER+DE +RRE+ V VRR VFE+SFR L R PEE KNRFYIVFE EEGQDAGGLLREWYVIISREIFNPMYALF SPGD+VTYMI SS NPNHL YFKFVGRVIAKA++DNKLLECYFTRSFYKHILGK VK+TDMES+DY FYKGL++L+ + +S LGY+LTFSTE+QEFGVT+IRDL PNGR+ VTEE K +Y++LVCQ+KM+G+IR+QL AFLEGFY+IIP+ LISIFNEQELELL+SGLP IDI+DLK+NTEYHKY S QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF LEGMNG+QKFQIHRDDRSTDRLP AHTCFNQLDLP Y++YDKLR+ LLKAIHECSE F
Sbjct: 4586 TLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERLDEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFGF 5150 HSP 2 Score: 251.136 bits (640), Expect = 3.725e-65 Identity = 157/399 (39.35%), Postives = 217/399 (54.39%), Query Frame = 0
Query: 1793 LTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQPRESEEAIGGXSXIASSNPIPSTSG--------TSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSN--VPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAF----------------LANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANE 2164
L +++PG + S +P VA+ S S T S +S + +P + +A G A++ ST+G T +P+ + LGD+E+PEGVDPSFLAALP EMR+EVI + R+Q IRQRAQQ + ++ + EVNPEFLAALP NIQ EVL QQR+EQQRQ +Q NPE PVD FFQ LP +LRQ++L DME+SQ+A+LP ELAAEAQ LRR+ E RN M +N + + L+ + R S + +AS A + +H LA + LLD + L+ LL+ LFV+D K+N+ RLHR++RNLC+H PTRDW++ L+SI+++ NE
Sbjct: 3557 LVHAEDDLWPGTVVSAGTREESSAPAVATESTEAVNESNQPEPTPESSESTSPNPQAPSAEPTPLEPGAAVDASVGAQTPATTEEAASTAGASGTTATITDMSPEVRAALGDLEVPEGVDPSFLAALPSEMREEVIQEHLRMQRIRQRAQQNAIQIAHDSLVEVNPEFLAALPLNIQSEVLMQQRIEQQRQAAQTANPEDPVDTAAFFQNLPENLRQAILTDMEESQIASLPPELAAEAQFLRRDWESRNGARMDAHPPSNALSRFQTTLQ----------------SLDDTRWHSSIWYDASGNAQPQHHQHTTIVHQHPHAHHAPNMGKPLALLMMEDENILLDPDSLATLLLFLFVEDQKVNSMRLHRVIRNLCHHEPTRDWIISALISIIQKTNE 3939 HSP 3 Score: 228.794 bits (582), Expect = 1.741e-58 Identity = 124/269 (46.10%), Postives = 168/269 (62.45%), Query Frame = 0
Query: 21 KYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSP-IRQRR-GQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGG---------KLSPEEGLESNE-----LPDGTGEFLDSWLMLLERMVNPKAVLESPHTL-PNLS-IGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEK 271
KY P Q + IK LL ASSRLGRALAEL LVKL VGSP RQRR + P+ A +A L+ +L +G S P+P +LTF CSVGFT PMLFDDK+ +HLM+ +F GLKAFF+ FYWALS S E+ ++S+E P+GTGEFLD+WL LLE+MVNP+A+L+SP+T+ P L+ + + PF+PV YLI H+LA A+ +IW + P+ YGP M E+++ IL H++K + + ++
Sbjct: 1179 KYTP--EEQFRYIKSLLAASSRLGRALAELLGTLVKLSVGSPQTRQRRINDLISNITKVPTPEAREIARILSSILVNGFSHESILPKPVPKLKLTFLICSVGFTGPMLFDDKRSAFHLMISQFCAENGLKAFFEMFYWALSLDNVSQKFEFDQWASMEDLMQSHEDDKRDCPEGTGEFLDAWLQLLEKMVNPRAMLDSPYTMSPRLNYMPDSVPFEPVFYLIHIHQLAMQALRRIWARRPIPNYGPSMFETMILILKHLIKSHQTLLDR 1445 HSP 4 Score: 115.161 bits (287), Expect = 4.145e-24 Identity = 89/378 (23.54%), Postives = 169/378 (44.71%), Query Frame = 0
Query: 386 GFSFYDLQILRDMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKS-VMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEK---SQSPIEEDVFRTTESNKAAVRIHLFILLFEE-----CKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCSGS------VGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK 748
G + ++ L DMGF +EA+ ++ EAT+YLL ++SS + + + A M +DVDV + E + S + L S + D F +A+ ++ +P+TV DL+ ++ R+ K+ + + + +D L +L E K S++ E+ + + VR+ LL EE + L I + ++V ++ L +S +L++ + + P+W+ L+ ID + + + R+A++ +V S W+WYD S+G+W Y+ A+N+ I +A+ +GE + I GR++ T+ M Q +E +G RP+ ++K
Sbjct: 1466 GLNADHIKTLTDMGFMHYHVIEALRTNASLEEATDYLLNNPEASSLSTTGGQSSSSAPPPPPPP----------SASTMDIDVDV---------PADGESTQSKSSTSPNSSYDYKHLKLMPSLIFDRFCDEAISRAFEFMEAIPDTVNSAADLIAVLYRRHNHLNKQVFVTNFVRNIIQWVDFTL-QLFEPAKTTGSKASRLEECLTGMTATRLFVRLKTSTLLLEENYSDLHRPLVEAITAQDAMVSLVKLLDCMSQWMLEQSTETNTGQQRATVPRWVQNLVDLIDAIDSISHILQRKANMRAVSSDVWRWYDASTGKWNAYSEANNELIRNAYAAGERWLYINIGRQRCTVSLNCMTQVSEASGTHRPVCPALK 1823 HSP 5 Score: 90.1225 bits (222), Expect = 1.560e-16 Identity = 50/141 (35.46%), Postives = 83/141 (58.87%), Query Frame = 0
Query: 824 AVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVI--RTSTLNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVA 962
++LRLC RLT+ ++ A F R GGV++LL L Q+ F G + A +++RHVLE P L+ ME+V+ R + + +++ Y+L ++ A R+P IFV+ AK++L+ E +D R++VKS + P + A
Sbjct: 1983 SLLRLCARLTRNYDNAQVFIRRGGVRMLLHLQQACSFMGFPTYANIILRHVLEAPPVLQEAMERVLGMRAAGI-VPVGHRDLIYVLTQVSSAVSRNPQIFVAAAKEMLKGEYLINTNSTTLADDARVMVKSKFGQQPTAAA 2122 HSP 6 Score: 88.1965 bits (217), Expect = 6.426e-16 Identity = 58/208 (27.88%), Postives = 105/208 (50.48%), Query Frame = 0
Query: 1049 KPFLSKSAVCRLLAELVKSYGGCA-KLITDHVY---ESGIVENIKEDTSALAFLLDELLTSKADK---DIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVS 1249
KP +S++ + +LLA+ ++Y +++ HVY +S ++ ++ + L LLD L ++ + + LI +L+ C P Q + E+ A+ R+L QP+S KH+++Q L L +IES P + NN+ +++L++G++T L +V DLS+ N T+ + L+P+E L R T L S
Sbjct: 2256 KPTISRATLLKLLADSTRAYQSLVPRVLLSHVYTPADSPLISAPQQ--TFLGVLLDRFLPITQRHHVPEVSTMTRVLICSLSDCYQQPGVQVHVAEELHAAVARALAQPDSAEKHTRLQVLMSLFPNLIES----------PMLYDKVHMHRNNMYRVLLRQGVMTYLTKVAQYKDLSNHNTLSTLAAILRPMEILLRFSVSTTTLGS 2451
BLAST of EMLSAG00000004991 vs. Select Arthropod Genomes
Match: AAF48495.2 (CG8184, isoform B [Drosophila melanogaster]) HSP 1 Score: 759.985 bits (1961), Expect = 0.000e+0 Identity = 365/565 (64.60%), Postives = 422/565 (74.69%), Query Frame = 0
Query: 2439 SLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKK------------------------KVQQKETRQEQLAHIQE----------QLADNKEMESSST------------ASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+LS+ L LD LW L +CL L E+ D AVLVLQ +VEAFFL+HA+ +K + QE + + D M+ +ST A S L D +KFL FAE HRTVLNQILRQS HL+DGPF+VLVDHTR+LDFD+KR+YF+TELER+DE +RRE+ V VRR VFE+SFR L R PEE KNRFYIVFE EEGQDAGGLLREWYVIISREIFNPMYALF SPGD+VTYMI SS NPNHL YFKFVGRVIAKA++DNKLLECYFTRSFYKHILGK VK+TDMES+DY FYKGL++L+ + +S LGY+LTFSTE+QEFGVT+IRDL PNGR+ VTEE K +Y++LVCQ+KM+G+IR+QL AFLEGFY+IIP+ LISIFNEQELELL+SGLP IDI+DLK+NTEYHKY S QI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF LEGMNG+QKFQIHRDDRSTDRLP AHTCFNQLDLP Y++YDKLR+ LLKAIHECSE F
Sbjct: 4581 TLSQILCLDVLWNTLSECLVALEESKDEFAVLVLQPTVEAFFLIHASHRGASKKSGRSFMPNAAGFGLRAAAGAGAIGGLGAAAPSAQEYSPFMDDVSSANTPSSASFDDEPRMQDASTNTPLVTNPSTSTAMSAHEAQLRQDRQKFLQFAEKHRTVLNQILRQSPTHLSDGPFAVLVDHTRILDFDVKRKYFQTELERLDEGIRREEHTVSVRRVTVFEDSFRVLYRLGPEEWKNRFYIVFEDEEGQDAGGLLREWYVIISREIFNPMYALFCVSPGDRVTYMINPSSHANPNHLSYFKFVGRVIAKAVHDNKLLECYFTRSFYKHILGKQVKHTDMESQDYEFYKGLDYLMKNDISTLGYELTFSTEVQEFGVTQIRDLKPNGRDTAVTEENKFEYVQLVCQLKMSGSIRQQLDAFLEGFYDIIPKHLISIFNEQELELLISGLPDIDIEDLKANTEYHKYTSKSAQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFGSLEGMNGIQKFQIHRDDRSTDRLPCAHTCFNQLDLPMYKSYDKLRSCLLKAIHECSEGFGF 5145 HSP 2 Score: 251.136 bits (640), Expect = 3.662e-65 Identity = 157/399 (39.35%), Postives = 217/399 (54.39%), Query Frame = 0
Query: 1793 LTEVPSNIYPGRDLPTVLDPLSPSPEVASTSGATTTSSTTTAGTDVSVTGSSSSTTGGDVSMESDQPRESEEAIGGXSXIASSNPIPSTSG--------TSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSN--VPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAF----------------LANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANE 2164
L +++PG + S +P VA+ S S T S +S + +P + +A G A++ ST+G T +P+ + LGD+E+PEGVDPSFLAALP EMR+EVI + R+Q IRQRAQQ + ++ + EVNPEFLAALP NIQ EVL QQR+EQQRQ +Q NPE PVD FFQ LP +LRQ++L DME+SQ+A+LP ELAAEAQ LRR+ E RN M +N + + L+ + R S + +AS A + +H LA + LLD + L+ LL+ LFV+D K+N+ RLHR++RNLC+H PTRDW++ L+SI+++ NE
Sbjct: 3552 LVHAEDDLWPGTVVSAGTREESSAPAVATESTEAVNESNQPEPTPESSESTSPNPQAPSAEPTPLEPGAAVDASVGAQTPATTEEAASTAGASGTTATITDMSPEVRAALGDLEVPEGVDPSFLAALPSEMREEVIQEHLRMQRIRQRAQQNAIQIAHDSLVEVNPEFLAALPLNIQSEVLMQQRIEQQRQAAQTANPEDPVDTAAFFQNLPENLRQAILTDMEESQIASLPPELAAEAQFLRRDWESRNGARMDAHPPSNALSRFQTTLQ----------------SLDDTRWHSSIWYDASGNAQPQHHQHTTIVHQHPHAHHAPNMGKPLALLMMEDENILLDPDSLATLLLFLFVEDQKVNSMRLHRVIRNLCHHEPTRDWIISALISIIQKTNE 3934 HSP 3 Score: 228.794 bits (582), Expect = 1.712e-58 Identity = 124/269 (46.10%), Postives = 168/269 (62.45%), Query Frame = 0
Query: 21 KYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSP-IRQRR-GQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGG---------KLSPEEGLESNE-----LPDGTGEFLDSWLMLLERMVNPKAVLESPHTL-PNLS-IGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEK 271
KY P Q + IK LL ASSRLGRALAEL LVKL VGSP RQRR + P+ A +A L+ +L +G S P+P +LTF CSVGFT PMLFDDK+ +HLM+ +F GLKAFF+ FYWALS S E+ ++S+E P+GTGEFLD+WL LLE+MVNP+A+L+SP+T+ P L+ + + PF+PV YLI H+LA A+ +IW + P+ YGP M E+++ IL H++K + + ++
Sbjct: 1180 KYTP--EEQFRYIKSLLAASSRLGRALAELLGTLVKLSVGSPQTRQRRINDLISNITKVPTPEAREIARILSSILVNGFSHESILPKPVPKLKLTFLICSVGFTGPMLFDDKRSAFHLMISQFCAENGLKAFFEMFYWALSLDNVSQKFEFDQWASMEDLMQSHEDDKRDCPEGTGEFLDAWLQLLEKMVNPRAMLDSPYTMSPRLNYMPDSVPFEPVFYLIHIHQLAMQALRRIWARRPIPNYGPSMFETMILILKHLIKSHQTLLDR 1446 HSP 4 Score: 115.161 bits (287), Expect = 4.075e-24 Identity = 89/378 (23.54%), Postives = 169/378 (44.71%), Query Frame = 0
Query: 386 GFSFYDLQILRDMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKS-VMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEK---SQSPIEEDVFRTTESNKAAVRIHLFILLFEE-----CKLLCAKIVEESSVVPIMTHLLTVSFDVLKRCSGS------VGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK 748
G + ++ L DMGF +EA+ ++ EAT+YLL ++SS + + + A M +DVDV + E + S + L S + D F +A+ ++ +P+TV DL+ ++ R+ K+ + + + +D L +L E K S++ E+ + + VR+ LL EE + L I + ++V ++ L +S +L++ + + P+W+ L+ ID + + + R+A++ +V S W+WYD S+G+W Y+ A+N+ I +A+ +GE + I GR++ T+ M Q +E +G RP+ ++K
Sbjct: 1467 GLNADHIKTLTDMGFMHYHVIEALRTNASLEEATDYLLNNPEASSLSTTGGQSSSSAPPPPPPP----------SASTMDIDVDV---------PADGESTQSKSSTSPNSSYDYKHLKLMPSLIFDRFCDEAISRAFEFMEAIPDTVNSAADLIAVLYRRHNHLNKQVFVTNFVRNIIQWVDFTL-QLFEPAKTTGSKASRLEECLTGMTATRLFVRLKTSTLLLEENYSDLHRPLVEAITAQDAMVSLVKLLDCMSQWMLEQSTETNTGQQRATVPRWVQNLVDLIDAIDSISHILQRKANMRAVSSDVWRWYDASTGKWNAYSEANNELIRNAYAAGERWLYINIGRQRCTVSLNCMTQVSEASGTHRPVCPALK 1824 HSP 5 Score: 90.1225 bits (222), Expect = 1.586e-16 Identity = 50/141 (35.46%), Postives = 83/141 (58.87%), Query Frame = 0
Query: 824 AVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVI--RTSTLNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVA 962
++LRLC RLT+ ++ A F R GGV++LL L Q+ F G + A +++RHVLE P L+ ME+V+ R + + +++ Y+L ++ A R+P IFV+ AK++L+ E +D R++VKS + P + A
Sbjct: 1984 SLLRLCARLTRNYDNAQVFIRRGGVRMLLHLQQACSFMGFPTYANIILRHVLEAPPVLQEAMERVLGMRAAGI-VPVGHRDLIYVLTQVSSAVSRNPQIFVAAAKEMLKGEYLINTNSTTLADDARVMVKSKFGQQPTAAA 2123 HSP 6 Score: 88.1965 bits (217), Expect = 6.871e-16 Identity = 58/208 (27.88%), Postives = 105/208 (50.48%), Query Frame = 0
Query: 1049 KPFLSKSAVCRLLAELVKSYGGCA-KLITDHVY---ESGIVENIKEDTSALAFLLDELLTSKADK---DIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVS 1249
KP +S++ + +LLA+ ++Y +++ HVY +S ++ ++ + L LLD L ++ + + LI +L+ C P Q + E+ A+ R+L QP+S KH+++Q L L +IES P + NN+ +++L++G++T L +V DLS+ N T+ + L+P+E L R T L S
Sbjct: 2251 KPTISRATLLKLLADSTRAYQSLVPRVLLSHVYTPADSPLISAPQQ--TFLGVLLDRFLPITQRHHVPEVSTMTRVLICSLSDCYQQPGVQVHVAEELHAAVARALAQPDSAEKHTRLQVLMSLFPNLIES----------PMLYDKVHMHRNNMYRVLLRQGVMTYLTKVAQYKDLSNHNTLSTLAAILRPMEILLRFSVSTTTLGS 2446
BLAST of EMLSAG00000004991 vs. nr
Match: gi|942418677|gb|JAN91268.1| (E3 ubiquitin protein ligase URE-B1 variant [Daphnia magna]) HSP 1 Score: 1061.6 bits (2744), Expect = 0.000e+0 Identity = 662/1476 (44.85%), Postives = 868/1476 (58.81%), Query Frame = 0
Query: 15 EKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSI-GNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKGEDDKNSGGGAMPSRVASRFANSFSNSPPFQMSLPDFLGDSFLSPVINNEAGGGXAAQGGEQPQXVQQPEQPPTLQPEQQQQSXSQNAPPVITPXATAENNDDVEPAPGFSFYDLQILRDMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQED-LMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSPIEEDVFRTTESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVL------------KRCSGSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIKSKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPNEEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTST-LNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFL-VKTPDICESSNNATVVESATELKNNKGGGAPNV------------------IRNMXTGDLPGVLXXPPVTITT-----AQPCDTSASKDM---KKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTS---KADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQP---AFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSARKSKNDVPDSSAEDSTSNNPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDE-SVEPDAEATEHDVSTTAESID------PNSESQLQTVEDTNDEDYDEMMDQL---------LERDGS--DNFTIIPQVGSNVNSDQDHHQDG----------SRFAGHGCQDDVDRAXESSQXDAAIEEETED 1414
++ + K P +++K +KPLL +SRLGRALAELF LLVKLCVGSPIR RR PPTP PS A AVA AL +LL SGL+W PP TSP P FRLTF CSVGFT PMLFD+KK PYHLML KFV+ GG AFFD FYWALS GG++ ++GLE +LP+GTGEFLD+WLMLLE+MVNPK +L+SPH LPN ++ G+ QPF P+KYLI TH+ AF AVMK+WGK PLKVYG RM ESVL+ILCHILKGE II EKL++ K E+ G A AS N + SP S P A G AA G S P V P A + + D V P LQ L DMGF R++C+EA+ T ++ +AT+YLL+ S + A A A ED +MRAIA+SL D ++ D KE E+ S LSK V+D+F QQAL GCL+L+D+LPETV+R C L++A+ RNG+ +++ +L L E+ + + L D L ++ S + V ++++ KA+VRIHLF LL EE K+ CA+ +EE +V + LL+ VL K G TPKW+ PLLL +DLYEK+ LG RRAS+ V S WKW+DI SG+WC YT A+NK IDDAFW+G+ SVR+T GRR+Y +QF +MIQ NEETGNRRP+M+S+K K A +SS S + N AM++ E EK+ P + GL D+ S L+K+CVG + IP D D L+A +RLCLRLT+ ++ A +FA LGGVK LL L+QSS FSG +LATLL+RH++E+P+TL HTMEKVIR T N+ ++KE ++++RVLAPAACR +F V K LR + S NKRG+ +ED RLL+K+LP + P P L V++ ++CDLL+ L + P E+AT L ++G GA V IRN + DL V + A+ ++++S++M ++P L KSA+CRLLAELV+SY GCA+L+ +H++ + E + E+TSALAFLLD LL S DKD LV+TL+A+LASCNH PEAQS LV EVK ALNR+L PES KH+++Q+LT L+STMIESCP++ Q QQP AFKQ MNN+VK MLKRGL+ DLAR+PH LD+SSP +A T+N+ALKPLE LSRIVN P A + R P + + P S + + +GT+T S+ TRAQ E S DAE TE DVS ES++ ES +ED D+ +++ L +E D S D+ + +D H+ +GH +DD D A + D EEETE+
Sbjct: 1070 DRPRSEKLTPAQQYRMKMVKPLLSTASRLGRALAELFGLLVKLCVGSPIRHRRAHQAPPTPIVPSPSAQAVAGALTQLLTSGLAWEPPVTSPTPKFRLTFLICSVGFTSPMLFDEKKLPYHLMLHKFVSCGGQAAFFDAFYWALSAGGRVPLDQGLEYADLPEGTGEFLDAWLMLLEKMVNPKNILDSPHQLPNKALHGSKQPFSPLKYLISTHRQAFDAVMKLWGKRPLKVYGARMTESVLTILCHILKGEVIIQEKLTQ-EKAEEPAAPSGVATS---ASSGDNVTTTSPAVGTSNPAI-------------AALGEAATGS----------------------SMGARRPTVDRPGAES-DADLVNPQ------HLQALMDMGFPRDQCVEALLFTTSLEQATDYLLSTPPSLLRAAQGAVAAAAAEAVSTAMDTDGNDEDAVMRAIALSLTAD------------SDRTDETVAVEKEHHEHMLYSEPLSKQVLDQFVQQALDGCLSLLDSLPETVYRVCSLVVAMVQRNGEAYRDSMLASLAKEIGNCVTSLQDHLDGVDNLVSKADA-VIESSDAMKASVRIHLFTLLCEEMKMACARQLEEKDLVLKLIRLLSSGQQVLAVKAKVNEGIDCKSVDGIGITPKWIAPLLLCLDLYEKMSLGTRRRASMEKVTSHVWKWFDIVSGKWCPYTAANNKIIDDAFWAGQNSVRVTAGRRRYLLQFSSMIQVNEETGNRRPMMLSLKEKPKEAAQDESS--SVDENAAMDVDENEKE--SPKGSPL-------SGLNLDQGSELIKSCVGLLCIPADPDTLHAAMRLCLRLTRHYQHAEKFASLGGVKQLLNLSQSSTFSGFLTLATLLLRHLVEEPETLHHTMEKVIRAYTGPNAAPSTKEFHFLMRVLAPAACRDVQLFSEVCKTFLRADLSLTNKRGD-EEDNRLLMKALPPR-PGCSLPQLSGVAREIVCDLLDALTIPVPP----------EETATPLPTSEGDGATPVTVPPARRFAGRNVRQQELIRNSSSSDLLNHEAEETVDDASRRDSLARKKESTSSEEMDRRRRPLLPKSAICRLLAELVRSYAGCARLVAEHIFPAKTSELVSEETSALAFLLDNLLPSCQTAGDKDSPALVRTLVASLASCNHAPEAQSCLVAEVKGALNRALTMPESNDKHNRLQSLTALLSTMIESCPATGLQPQQPLTTAFKQQ-NLGMNNMVKFMLKRGLVNDLARIPHCLDMSSPLMAFTVNAALKPLEALSRIVNLPPAAPVAGR------PVTKPKSGDEGEPQESSAQL-------------QGTST--SDNTRAQAVEISNALDAENTEQDVSAAGESLENLADNEGGGESGDNNLEDIMDQHSQALVEALIVAAENPSQMETDDSAHDSQMRTHNESEFIGADDAHYHGNDPRDSSDSDSQSESGHS-EDDDDEADDDDDDDDGGEEETEE 2440 HSP 2 Score: 879.011 bits (2270), Expect = 0.000e+0 Identity = 428/592 (72.30%), Postives = 489/592 (82.60%), Query Frame = 0
Query: 2381 PAESTTTNTTTAPASTTTSTSDTTTIADQTNE--ENKMDVDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEK-KKVQQKETRQEQLAHIQE------------QLADNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
P+E+ + + AS ST + A+ TNE M+VDE +S + + LS+QL LDSLW L +CL++LA+TPDHHAVLVLQ +VEAFFLVHAA T +E+ K+ ++ ETR+ QL+H+Q+ Q +++ + + + S + + L PDT+KFL+FAE HRTVLNQILRQST HLADGPF+VLVDHTRVLDFD+KRRYFRTELER D +RREDLAVHV+RE+VFE+SFREL RRSPEE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF TSPGD+VTYMI S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGK V+YTDMESEDY+FY+GL FL+ H+V++LGYDLTFSTE+QEFGVTE RDL PNGRNI VTEE K++YIRLVCQMKMTGAIRKQL+AFLEGFY+IIP+RLISIFNEQELELLLSGLP IDIDDLK+N+EYHKYQ SLQI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF+ LEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE F
Sbjct: 3832 PSETLISEASGVAASVGASTGSEAS-AEVTNEIPATPMEVDEVNS--------SRDIDLPRLSQQLLLDSLWNTLSECLQELADTPDHHAVLVLQPAVEAFFLVHAAQTSKEEREKRSRRPETRESQLSHLQQEIGGPGSPSMAVQTSNDDSLNQLTLSMSSAPSDLPPDTQKFLSFAERHRTVLNQILRQSTTHLADGPFAVLVDHTRVLDFDVKRRYFRTELERADAGIRREDLAVHVKREHVFEDSFRELHRRSPEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFSTSPGDRVTYMINALSHCNSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKCVRYTDMESEDYSFYQGLAFLIEHEVTELGYDLTFSTEVQEFGVTEARDLKPNGRNIIVTEENKMEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLLSGLPNIDIDDLKANSEYHKYQPTSLQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFSALEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEGFGF 4414 HSP 3 Score: 320.087 bits (819), Expect = 1.924e-83 Identity = 191/368 (51.90%), Postives = 242/368 (65.76%), Query Frame = 0
Query: 1835 GTDVSVTGSSSSTTGGDVSMESDQPRESEEAIGGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSN---VPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFF----NNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNK-----SKRSSTATP 2190
GT++ + G+SS DVS S P P+ + Q+ +Y S+LG++EIPEGVDPSFLAALPE+MRQEVI +Q RLQ +RQRA+ Q T+ +N V EV+ EFLAALPPNIQEEVLAQQR+EQQR + NP PVDP +F TLP +LRQS+LADMEDSQMA LP ELAAEAQ+LRRE E RNR M +RFF N+G N+LSSI+R S+ RLG++Y + + R + + A A AN +R +GR LLD+E LSCLL+LLF+D+ K+NTTRLHR+LRNLCYH PTRDWVV+ L+SILER+NE + A + P+ + R+ TATP
Sbjct: 3012 GTNMEL-GASSGMARADVSGASALP-----------------PVVAGGSDQQSGEYQSLLGNIEIPEGVDPSFLAALPEDMRQEVIAEQLRLQRLRQRARAQATDTTNQQAVLEVSAEFLAALPPNIQEEVLAQQRLEQQRTVVPANPNEPVDPADFLSTLPPALRQSILADMEDSQMAVLPPELAAEAQNLRREWEARNRQLMTDRFFTHVGNSGANALSSIIRNSMGRLGSRYGVHPVHNRGHW----GGWGRPVAGASGSSAVAAQAAANGVRLRGRHLLDHEALSCLLVLLFIDEPKLNTTRLHRVLRNLCYHGPTRDWVVKSLLSILERSNEARANAAELFPITSTPSTKGKRITSRALTATP 3357 HSP 4 Score: 113.62 bits (283), Expect = 5.674e-21 Identity = 63/122 (51.64%), Postives = 81/122 (66.39%), Query Frame = 0
Query: 2261 HLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIK--------------------STNFEHDRSGKDKGGLLDRFTKETVVLNAPSK 2362
HL+LAVDVLTSKSCSEEGLED T+LLL+LS+GP PTRD IL+LLL GA+ELGN+V +++ L NEL +++ S E D + KG L+DRFTK+ VV+ A +K
Sbjct: 3634 HLKLAVDVLTSKSCSEEGLEDATSLLLSLSYGPPPTRDTILKLLLDGARELGNIVCSHIRALLNELHTLRRRPEASTSAASAQPVQSGTSSEEVEADGVDRSKGFLMDRFTKDMVVVAAQTK 3755
BLAST of EMLSAG00000004991 vs. nr
Match: gi|942357829|gb|JAN60844.1| (E3 ubiquitin protein ligase URE-B1 variant [Daphnia magna]) HSP 1 Score: 1061.21 bits (2743), Expect = 0.000e+0 Identity = 662/1476 (44.85%), Postives = 868/1476 (58.81%), Query Frame = 0
Query: 15 EKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSI-GNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKGEDDKNSGGGAMPSRVASRFANSFSNSPPFQMSLPDFLGDSFLSPVINNEAGGGXAAQGGEQPQXVQQPEQPPTLQPEQQQQSXSQNAPPVITPXATAENNDDVEPAPGFSFYDLQILRDMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQED-LMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSPIEEDVFRTTESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVL------------KRCSGSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIKSKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPNEEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTST-LNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFL-VKTPDICESSNNATVVESATELKNNKGGGAPNV------------------IRNMXTGDLPGVLXXPPVTITT-----AQPCDTSASKDM---KKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTS---KADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQP---AFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSARKSKNDVPDSSAEDSTSNNPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDE-SVEPDAEATEHDVSTTAESID------PNSESQLQTVEDTNDEDYDEMMDQL---------LERDGS--DNFTIIPQVGSNVNSDQDHHQDG----------SRFAGHGCQDDVDRAXESSQXDAAIEEETED 1414
++ + K P +++K +KPLL +SRLGRALAELF LLVKLCVGSPIR RR PPTP PS A AVA AL +LL SGL+W PP TSP P FRLTF CSVGFT PMLFD+KK PYHLML KFV+ GG AFFD FYWALS GG++ ++GLE +LP+GTGEFLD+WLMLLE+MVNPK +L+SPH LPN ++ G+ QPF P+KYLI TH+ AF AVMK+WGK PLKVYG RM ESVL+ILCHILKGE II EKL++ K E+ G A AS N + SP S P A G AA G S P V P A + + D V P LQ L DMGF R++C+EA+ T ++ +AT+YLL+ S + A A A ED +MRAIA+SL D ++ D KE E+ S LSK V+D+F QQAL GCL+L+D+LPETV+R C L++A+ RNG+ +++ +L L E+ + + L D L ++ S + V ++++ KA+VRIHLF LL EE K+ CA+ +EE +V + LL+ VL K G TPKW+ PLLL +DLYEK+ LG RRAS+ V S WKW+DI SG+WC YT A+NK IDDAFW+G+ SVR+T GRR+Y +QF +MIQ NEETGNRRP+M+S+K K A +SS S + N AM++ E EK+ P + GL D+ S L+K+CVG + IP D D L+A +RLCLRLT+ ++ A +FA LGGVK LL L+QSS FSG +LATLL+RH++E+P+TL HTMEKVIR T N+ ++KE ++++RVLAPAACR +F V K LR + S NKRG+ +ED RLL+K+LP + P P L V++ ++CDLL+ L + P E+AT L ++G GA V IRN + DL V + A+ ++++S++M ++P L KSA+CRLLAELV+SY GCA+L+ +H++ + E + E+TSALAFLLD LL S DKD LV+TL+A+LASCNH PEAQS LV EVK ALNR+L PES KH+++Q+LT L+STMIESCP++ Q QQP AFKQ MNN+VK MLKRGL+ DLAR+PH LD+SSP +A T+N+ALKPLE LSRIVN P A + R P + + P S + + +GT+T S+ TRAQ E S DAE TE DVS ES++ ES +ED D+ +++ L +E D S D+ + +D H+ +GH +DD D A + D EEETE+
Sbjct: 1070 DRPRSEKLTPAQQYRMKMVKPLLSTASRLGRALAELFGLLVKLCVGSPIRHRRAHQAPPTPIVPSPSAQAVAGALTQLLTSGLAWEPPVTSPTPKFRLTFLICSVGFTSPMLFDEKKLPYHLMLHKFVSCGGQAAFFDAFYWALSAGGRVPLDQGLEYADLPEGTGEFLDAWLMLLEKMVNPKNILDSPHQLPNKALHGSKQPFSPLKYLISTHRQAFDAVMKLWGKRPLKVYGARMTESVLTILCHILKGEVIIQEKLTQ-EKAEEPAAPSGVATS---ASSGDNVTTTSPAVGTSNPAI-------------AALGEAATGS----------------------SMGARRPTVDRPGAES-DADLVNPQ------HLQALMDMGFPRDQCVEALLFTTSLEQATDYLLSTPPSLLRAAQGAVAAAAAEAVSTAMDTDGNDEDAVMRAIALSLTAD------------SDRTDETVAVEKEHHEHMLYSEPLSKQVLDQFVQQALDGCLSLLDSLPETVYRVCSLVVAMVQRNGEAYRDSMLASLAKEIGNCVTSLQDHLDGVDNLVSKADA-VIESSDAMKASVRIHLFTLLCEEMKMACARQLEEKDLVLKLIRLLSSGQQVLAVKAKVNEGIDCKSVDGIGITPKWIAPLLLCLDLYEKMSLGTRRRASMEKVTSHVWKWFDIVSGKWCPYTAANNKIIDDAFWAGQNSVRVTAGRRRYLLQFSSMIQVNEETGNRRPMMLSLKEKPKEAAQDESS--SVDENAAMDVDENEKE--SPKGSPL-------SGLNLDQGSELIKSCVGLLCIPADPDTLHAAMRLCLRLTRHYQHAEKFASLGGVKQLLNLSQSSTFSGFLTLATLLLRHLVEEPETLHHTMEKVIRAYTGPNAAPSTKEFHFLMRVLAPAACRDVQLFSEVCKTFLRADLSLTNKRGD-EEDNRLLMKALPPR-PGCSLPQLSGVAREIVCDLLDALTIPVPP----------EETATPLPTSEGDGATPVTVPPARRFAGRNVRQQELIRNSSSSDLLNHEAEETVDDASRRDSLARKKESTSSEEMDRRRRPLLPKSAICRLLAELVRSYAGCARLVAEHIFPAKTSELVSEETSALAFLLDNLLPSCQTAGDKDSPALVRTLVASLASCNHAPEAQSCLVAEVKGALNRALTMPESNDKHNRLQSLTALLSTMIESCPATGLQPQQPLTTAFKQQ-NLGMNNMVKFMLKRGLVNDLARIPHCLDMSSPLMAFTVNAALKPLEALSRIVNLPPAAPVAGR------PVTKPKSGDEGEPQESSAQL-------------QGTST--SDNTRAQAVEISNALDAENTEQDVSAAGESLENLADNEGGGESGDNNLEDIMDQHSQALVEALIVAAENPSQMETDDSAHDSQMRTHNESEFIGADDAHYHGNDPRDSSDSDSQSESGHS-EDDDDEADDDDDDDDGGEEETEE 2440 HSP 2 Score: 879.011 bits (2270), Expect = 0.000e+0 Identity = 428/592 (72.30%), Postives = 489/592 (82.60%), Query Frame = 0
Query: 2381 PAESTTTNTTTAPASTTTSTSDTTTIADQTNE--ENKMDVDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEK-KKVQQKETRQEQLAHIQE------------QLADNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
P+E+ + + AS ST + A+ TNE M+VDE +S + + LS+QL LDSLW L +CL++LA+TPDHHAVLVLQ +VEAFFLVHAA T +E+ K+ ++ ETR+ QL+H+Q+ Q +++ + + + S + + L PDT+KFL+FAE HRTVLNQILRQST HLADGPF+VLVDHTRVLDFD+KRRYFRTELER D +RREDLAVHV+RE+VFE+SFREL RRSPEE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF TSPGD+VTYMI S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGK V+YTDMESEDY+FY+GL FL+ H+V++LGYDLTFSTE+QEFGVTE RDL PNGRNI VTEE K++YIRLVCQMKMTGAIRKQL+AFLEGFY+IIP+RLISIFNEQELELLLSGLP IDIDDLK+N+EYHKYQ SLQI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF+ LEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE F
Sbjct: 3839 PSETLISEASGVAASVGASTGSEAS-AEVTNEIPATPMEVDEVNS--------SRDIDLPRLSQQLLLDSLWNTLSECLQELADTPDHHAVLVLQPAVEAFFLVHAAQTSKEEREKRSRRPETRESQLSHLQQEIGGPGSPSMAVQTSNDDSLNQLTLSMSSAPSDLPPDTQKFLSFAERHRTVLNQILRQSTTHLADGPFAVLVDHTRVLDFDVKRRYFRTELERADAGIRREDLAVHVKREHVFEDSFRELHRRSPEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFSTSPGDRVTYMINALSHCNSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKCVRYTDMESEDYSFYQGLAFLIEHEVTELGYDLTFSTEVQEFGVTEARDLKPNGRNIIVTEENKMEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLLSGLPNIDIDDLKANSEYHKYQPTSLQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFSALEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEGFGF 4421 HSP 3 Score: 320.857 bits (821), Expect = 1.144e-83 Identity = 191/368 (51.90%), Postives = 242/368 (65.76%), Query Frame = 0
Query: 1835 GTDVSVTGSSSSTTGGDVSMESDQPRESEEAIGGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSN---VPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFF----NNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNK-----SKRSSTATP 2190
GT++ + G+SS DVS S P P+ + Q+ +Y S+LG++EIPEGVDPSFLAALPE+MRQEVI +Q RLQ +RQRA+ Q T+ +N V EV+ EFLAALPPNIQEEVLAQQR+EQQR + NP PVDP +F TLP +LRQS+LADMEDSQMA LP ELAAEAQ+LRRE E RNR M +RFF N+G N+LSSI+R S+ RLG++Y + + R + + A A AN +R +GR LLD+E LSCLL+LLF+D+ K+NTTRLHR+LRNLCYH PTRDWVV+ L+SILER+NE + A + P+ + R+ TATP
Sbjct: 3012 GTNMEL-GASSGMARADVSGASALPXXX----------XXXXPVVAGGSDQQSGEYQSLLGNIEIPEGVDPSFLAALPEDMRQEVIAEQLRLQRLRQRARAQATDTTNQQAVLEVSAEFLAALPPNIQEEVLAQQRLEQQRTVVPANPNEPVDPADFLSTLPPALRQSILADMEDSQMAVLPPELAAEAQNLRREWEARNRQLMTDRFFTHVGNSGANALSSIIRNSMGRLGSRYGVHPVHNRGHW----GGWGRPVAGASGSSAVAAQAAANGVRLRGRHLLDHEALSCLLVLLFIDEPKLNTTRLHRVLRNLCYHGPTRDWVVKSLLSILERSNEARANAAELFPITSTPSTKGKRITSRALTATP 3364 HSP 4 Score: 113.62 bits (283), Expect = 5.628e-21 Identity = 63/122 (51.64%), Postives = 81/122 (66.39%), Query Frame = 0
Query: 2261 HLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIK--------------------STNFEHDRSGKDKGGLLDRFTKETVVLNAPSK 2362
HL+LAVDVLTSKSCSEEGLED T+LLL+LS+GP PTRD IL+LLL GA+ELGN+V +++ L NEL +++ S E D + KG L+DRFTK+ VV+ A +K
Sbjct: 3641 HLKLAVDVLTSKSCSEEGLEDATSLLLSLSYGPPPTRDTILKLLLDGARELGNIVCSHIRALLNELHTLRRRPEASTSAASAQPVQSGTSSEEVEADGVDRSKGFLMDRFTKDMVVVAAQTK 3762
BLAST of EMLSAG00000004991 vs. nr
Match: gi|1022762020|gb|KZS07874.1| (E3 ubiquitin-protein ligase HUWE1 [Daphnia magna]) HSP 1 Score: 1061.21 bits (2743), Expect = 0.000e+0 Identity = 662/1476 (44.85%), Postives = 868/1476 (58.81%), Query Frame = 0
Query: 15 EKKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSI-GNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKGEDDKNSGGGAMPSRVASRFANSFSNSPPFQMSLPDFLGDSFLSPVINNEAGGGXAAQGGEQPQXVQQPEQPPTLQPEQQQQSXSQNAPPVITPXATAENNDDVEPAPGFSFYDLQILRDMGFSRERCLEAMDRTGAMYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQED-LMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSPIEEDVFRTTESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVL------------KRCSGSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIKSKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPNEEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTST-LNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFL-VKTPDICESSNNATVVESATELKNNKGGGAPNV------------------IRNMXTGDLPGVLXXPPVTITT-----AQPCDTSASKDM---KKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTS---KADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQP---AFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSARKSKNDVPDSSAEDSTSNNPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDE-SVEPDAEATEHDVSTTAESID------PNSESQLQTVEDTNDEDYDEMMDQL---------LERDGS--DNFTIIPQVGSNVNSDQDHHQDG----------SRFAGHGCQDDVDRAXESSQXDAAIEEETED 1414
++ + K P +++K +KPLL +SRLGRALAELF LLVKLCVGSPIR RR PPTP PS A AVA AL +LL SGL+W PP TSP P FRLTF CSVGFT PMLFD+KK PYHLML KFV+ GG AFFD FYWALS GG++ ++GLE +LP+GTGEFLD+WLMLLE+MVNPK +L+SPH LPN ++ G+ QPF P+KYLI TH+ AF AVMK+WGK PLKVYG RM ESVL+ILCHILKGE II EKL++ K E+ G A AS N + SP S P A G AA G S P V P A + + D V P LQ L DMGF R++C+EA+ T ++ +AT+YLL+ S + A A A ED +MRAIA+SL D ++ D KE E+ S LSK V+D+F QQAL GCL+L+D+LPETV+R C L++A+ RNG+ +++ +L L E+ + + L D L ++ S + V ++++ KA+VRIHLF LL EE K+ CA+ +EE +V + LL+ VL K G TPKW+ PLLL +DLYEK+ LG RRAS+ V S WKW+DI SG+WC YT A+NK IDDAFW+G+ SVR+T GRR+Y +QF +MIQ NEETGNRRP+M+S+K K A +SS S + N AM++ E EK+ P + GL D+ S L+K+CVG + IP D D L+A +RLCLRLT+ ++ A +FA LGGVK LL L+QSS FSG +LATLL+RH++E+P+TL HTMEKVIR T N+ ++KE ++++RVLAPAACR +F V K LR + S NKRG+ +ED RLL+K+LP + P P L V++ ++CDLL+ L + P E+AT L ++G GA V IRN + DL V + A+ ++++S++M ++P L KSA+CRLLAELV+SY GCA+L+ +H++ + E + E+TSALAFLLD LL S DKD LV+TL+A+LASCNH PEAQS LV EVK ALNR+L PES KH+++Q+LT L+STMIESCP++ Q QQP AFKQ MNN+VK MLKRGL+ DLAR+PH LD+SSP +A T+N+ALKPLE LSRIVN P A + R P + + P S + + +GT+T S+ TRAQ E S DAE TE DVS ES++ ES +ED D+ +++ L +E D S D+ + +D H+ +GH +DD D A + D EEETE+
Sbjct: 1065 DRPRSEKLTPAQQYRMKMVKPLLSTASRLGRALAELFGLLVKLCVGSPIRHRRAHQAPPTPIVPSPSAQAVAGALTQLLTSGLAWEPPVTSPTPKFRLTFLICSVGFTSPMLFDEKKLPYHLMLHKFVSCGGQAAFFDAFYWALSAGGRVPLDQGLEYADLPEGTGEFLDAWLMLLEKMVNPKNILDSPHQLPNKALHGSKQPFSPLKYLISTHRQAFDAVMKLWGKRPLKVYGARMTESVLTILCHILKGEVIIQEKLTQ-EKAEEPAAPSGVATS---ASSGDNVTTTSPAVGTSNPAI-------------AALGEAATGS----------------------SMGARRPTVDRPGAES-DADLVNPQ------HLQALMDMGFPRDQCVEALLFTTSLEQATDYLLSTPPSLLRAAQGAVAAAAAEAVSTAMDTDGNDEDAVMRAIALSLTAD------------SDRTDETVAVEKEHHEHMLYSEPLSKQVLDQFVQQALDGCLSLLDSLPETVYRVCSLVVAMVQRNGEAYRDSMLASLAKEIGNCVTSLQDHLDGVDNLVSKADA-VIESSDAMKASVRIHLFTLLCEEMKMACARQLEEKDLVLKLIRLLSSGQQVLAVKAKVNEGIDCKSVDGIGITPKWIAPLLLCLDLYEKMSLGTRRRASMEKVTSHVWKWFDIVSGKWCPYTAANNKIIDDAFWAGQNSVRVTAGRRRYLLQFSSMIQVNEETGNRRPMMLSLKEKPKEAAQDESS--SVDENAAMDVDENEKE--SPKGSPL-------SGLNLDQGSELIKSCVGLLCIPADPDTLHAAMRLCLRLTRHYQHAEKFASLGGVKQLLNLSQSSTFSGFLTLATLLLRHLVEEPETLHHTMEKVIRAYTGPNAAPSTKEFHFLMRVLAPAACRDVQLFSEVCKTFLRADLSLTNKRGD-EEDNRLLMKALPPR-PGCSLPQLSGVAREIVCDLLDALTIPVPP----------EETATPLPTSEGDGATPVTVPPARRFAGRNVRQQELIRNSSSSDLLNHEAEETVDDASRRDSLARKKESTSSEEMDRRRRPLLPKSAICRLLAELVRSYAGCARLVAEHIFPAKTSELVSEETSALAFLLDNLLPSCQTAGDKDSPALVRTLVASLASCNHAPEAQSCLVAEVKGALNRALTMPESNDKHNRLQSLTALLSTMIESCPATGLQPQQPLTTAFKQQ-NLGMNNMVKFMLKRGLVNDLARIPHCLDMSSPLMAFTVNAALKPLEALSRIVNLPPAAPVAGR------PVTKPKSGDEGEPQESSAQL-------------QGTST--SDNTRAQAVEISNALDAENTEQDVSAAGESLENLADNEGGGESGDNNLEDIMDQHSQALVEALIVAAENPSQMETDDSAHDSQMRTHNESEFIGADDAHYHGNDPRDSSDSDSQSESGHS-EDDDDEADDDDDDDDGGEEETEE 2435 HSP 2 Score: 879.011 bits (2270), Expect = 0.000e+0 Identity = 428/592 (72.30%), Postives = 489/592 (82.60%), Query Frame = 0
Query: 2381 PAESTTTNTTTAPASTTTSTSDTTTIADQTNE--ENKMDVDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEK-KKVQQKETRQEQLAHIQE------------QLADNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
P+E+ + + AS ST + A+ TNE M+VDE +S + + LS+QL LDSLW L +CL++LA+TPDHHAVLVLQ +VEAFFLVHAA T +E+ K+ ++ ETR+ QL+H+Q+ Q +++ + + + S + + L PDT+KFL+FAE HRTVLNQILRQST HLADGPF+VLVDHTRVLDFD+KRRYFRTELER D +RREDLAVHV+RE+VFE+SFREL RRSPEE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF TSPGD+VTYMI S N NHL YFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGK V+YTDMESEDY+FY+GL FL+ H+V++LGYDLTFSTE+QEFGVTE RDL PNGRNI VTEE K++YIRLVCQMKMTGAIRKQL+AFLEGFY+IIP+RLISIFNEQELELLLSGLP IDIDDLK+N+EYHKYQ SLQI WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF+ LEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSE F
Sbjct: 3780 PSETLISEASGVAASVGASTGSEAS-AEVTNEIPATPMEVDEVNS--------SRDIDLPRLSQQLLLDSLWNTLSECLQELADTPDHHAVLVLQPAVEAFFLVHAAQTSKEEREKRSRRPETRESQLSHLQQEIGGPGSPSMAVQTSNDDSLNQLTLSMSSAPSDLPPDTQKFLSFAERHRTVLNQILRQSTTHLADGPFAVLVDHTRVLDFDVKRRYFRTELERADAGIRREDLAVHVKREHVFEDSFRELHRRSPEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFSTSPGDRVTYMINALSHCNSNHLCYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKCVRYTDMESEDYSFYQGLAFLIEHEVTELGYDLTFSTEVQEFGVTEARDLKPNGRNIIVTEENKMEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLLSGLPNIDIDDLKANSEYHKYQPTSLQIQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFSALEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEGFGF 4362 HSP 3 Score: 279.256 bits (713), Expect = 4.722e-71 Identity = 167/361 (46.26%), Postives = 211/361 (58.45%), Query Frame = 0
Query: 1835 GTDVSVTGSSSSTTGGDVSMESDQPRESEEAIGGXSXIASSNPIPSTSGTSQNPDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNK-----SKRSSTATP 2190
GT++ + G+SS DVS S P P+ + Q+ +Y S+LG++EIPEGVDPSFLAALPE+MRQE V EV+ EFLAALPPNIQEEVLAQQR+EQQR + NP PVDP +F TLP +LRQS+LADMEDSQMA LP ELAAEAQ+LRR N+LSSI+R S+ RLG++Y + + R + + A A AN +R +GR LLD+E LSCLL+LLF+D+ K+NTTRLHR+LRNLCYH PTRDWVV+ L+SILER+NE + A + P+ + R+ TATP
Sbjct: 3007 GTNMEL-GASSGMARADVSGASALP-----------------PVVAGGSDQQSGEYQSLLGNIEIPEGVDPSFLAALPEDMRQE----------------------QAVLEVSAEFLAALPPNIQEEVLAQQRLEQQRTVVPANPNEPVDPADFLSTLPPALRQSILADMEDSQMAVLPPELAAEAQNLRR------------------ANALSSIIRNSMGRLGSRYGVHPVHNRGHW----GGWGRPVAGASGSSAVAAQAAANGVRLRGRHLLDHEALSCLLVLLFIDEPKLNTTRLHRVLRNLCYHGPTRDWVVKSLLSILERSNEARANAAELFPITSTPSTKGKRITSRALTATP 3305 HSP 4 Score: 113.62 bits (283), Expect = 5.618e-21 Identity = 63/122 (51.64%), Postives = 81/122 (66.39%), Query Frame = 0
Query: 2261 HLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIK--------------------STNFEHDRSGKDKGGLLDRFTKETVVLNAPSK 2362
HL+LAVDVLTSKSCSEEGLED T+LLL+LS+GP PTRD IL+LLL GA+ELGN+V +++ L NEL +++ S E D + KG L+DRFTK+ VV+ A +K
Sbjct: 3582 HLKLAVDVLTSKSCSEEGLEDATSLLLSLSYGPPPTRDTILKLLLDGARELGNIVCSHIRALLNELHTLRRRPEASTSTASAQPVQSGTSSEEVEADGVDRSKGFLMDRFTKDMVVVAAQTK 3703
BLAST of EMLSAG00000004991 vs. nr
Match: gi|1048067138|ref|XP_017486096.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1-like [Rhagoletis zephyria]) HSP 1 Score: 1037.33 bits (2681), Expect = 0.000e+0 Identity = 574/1158 (49.57%), Postives = 742/1158 (64.08%), Query Frame = 0
Query: 1888 PDYSSILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVP----EVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSVSRLGNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKD------------------IMASIQSSFQLPNKSK---RSSTATPVKSASSQQQQQXKYPVIRRN-----------------------------------SLLT-----------DKLLRLLSLISIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADH-LRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQ---NELLS--------IKSTNFEHDRSGKDKGGLLDRFTKETVVLNAPSKQXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEENKMDVDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQK-ETRQEQLAHIQEQLADNKEMESSST---ASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
P+ +ILGD EIP+GVDPSFLAALPE +RQEVI +Q RLQ + ++ N + P EVNPEFLAALPPNIQEEVLAQQR EQQR +Q VNPEAPVDP FF++LP SLR+ VL+D++DSQ+ L ++A EA++LR+E E R+R F ER F++ + + G+ R F S SRF +A+ + R KG+ LLD + LS LLILLF+ D+ +N+ RLHR++RNLC+HAPTR WV++ L+ I+ER E+ D + +S ++ PN S +S + V S SS + + V+ R+ SLL+ D+L+RLL+ ++ + + + ++ + G L ++ + L+L VDVL SK CSEEGL D T+LL+ L+ R I +LLL G + LG+ V +++ L ELL+ + + D++ KG +LDR++ +++V++A + + S P+ S + +++ TI + +NK N S SE K + + LS +L LD LW+ L +CL L+E PD HAVLVLQ +VE+FFLVHA P++ K + + E+ QLAHI + +A ++ S +T + + +L PDT+KFL FAE HR VLNQILRQS+ HLA GPFSVLVDHTR+LDFD+KRRYFR ELER+D +RR+DLAVH+RRENVFE+SFREL RRS EE KNRFY+VFEGEEGQDAGGLLREWY IISREIFNP YALF TSPGD+VTYMI +SS N NHL YFKFVGR+I KAI+DNKLL+CYFTRSFYKHILGK VKY D+E++D A YKGL +L+ H V+D+G ++ F E+ +FGV + +L P+GRNI VTE K DY++ +CQ KMT +IR QLSAFLEGFY ++P++LISIFNE ELELL+SGLP IDIDDLK+N+EYHKY NSLQ+ WFWRALRSFDQ D+AKF+QFVTG+SKVPLQGF+ LEGMNG QKFQIH DDRSTDRLPSAHTCFNQLDLPAYETYDKLR LLKAIHECSE F
Sbjct: 2815 PEERAILGDQEIPDGVDPSFLAALPENIRQEVIAEQFRLQRLNKQLATANASTNLAPATFAEVNPEFLAALPPNIQEEVLAQQRAEQQRLAAQSVNPEAPVDPDSFFRSLPPSLRRQVLSDLDDSQIRLLSVDIANEARALRQENEIRHRQF-QERLFSSSTGAGGGAHSNGTAAAGH-------YNRNTF--SASRFPPRNAS-VGRT------------IKGKHLLDFDSLSSLLILLFIHDNLLNSQRLHRLIRNLCFHAPTRQWVIQSLLEIMERTKESVDKSYDSVHSSELISNDDLSVNSSSKNPIVTPNTSTPTLKSESQEAVSSPSSSKNTPNFFDVLMRHDSIHANKKKSLKHIQAIDGTDGSKTIEQSPFSQLLSLLSHQFIKKSSMLTDRLMRLLTHVTYSMYAEF---ESRNASTTETKEGKFAELEKSLANEKLLKLVVDVLISKGCSEEGLIDATSLLIKLATVFSSCRTIFYKLLLDGVRGLGDSVFSDINALSFELKELLAKMRKDTPVVDNAGDSGDKASSSKGLILDRYSSQSIVVSAVAT------SKHNISGKEVQLPSMSALISKSSSQFLFLRILKIIITIRGIASSKNKASTPTNQS---SETKMEVDKEEDKLSSELHLDHLWDKLSECLCLLSEAPDDHAVLVLQPAVESFFLVHA----PEKANKTETRAESVSNQLAHISQSMATSETFPSDATQVDSENIQMANLPPDTQKFLFFAEKHRVVLNQILRQSSVHLAAGPFSVLVDHTRILDFDVKRRYFRQELERLDHGVRRDDLAVHIRRENVFEDSFRELNRRSSEEWKNRFYVVFEGEEGQDAGGLLREWYTIISREIFNPNYALFTTSPGDRVTYMINQSSYCNTNHLSYFKFVGRLIGKAIFDNKLLDCYFTRSFYKHILGKVVKYNDLEAQDNALYKGLVYLLEHNVADMGTEMRFVYEVDDFGVVKTIELAPDGRNIVVTEANKHDYVKYMCQAKMTNSIRAQLSAFLEGFYALVPKKLISIFNEHELELLISGLPNIDIDDLKNNSEYHKYSTNSLQVQWFWRALRSFDQADRAKFLQFVTGTSKVPLQGFSALEGMNGPQKFQIHLDDRSTDRLPSAHTCFNQLDLPAYETYDKLRLMLLKAIHECSEGFGF 3933 HSP 2 Score: 451.44 bits (1160), Expect = 2.216e-123 Identity = 336/1011 (33.23%), Postives = 501/1011 (49.55%), Query Frame = 0
Query: 392 LQILRDMGFSRERCLEAMDRTGA-MYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSG----KEEEENEGGSFGLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLL----ELEKSQSPIEEDVFRTTESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLKRC---SGSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSV-CSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIKSKGSRDAVVKSSATSSNSNNAMELGEEEKKVSDPNEEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKR-GETDEDTRLLVKSLPTKA---PNSVAPPLGEVSQSVICDLLNFLVKTPDICESSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKD----MKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTS------KADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALV-SSARKSKNDVPDSS--AEDSTSNNPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERDGSDN-FTIIPQVGS 1371
L IL DMGFSRE A+ T + +A E L+ S + + SS A N + E++ +++AIAMSLD E D + G K L KS +D FT L G L L+D LP+TV++ C+LL+AV RNG + + ++ LI E+ I L+ +LEKS + E+ K + RIHL LLF E K CA + +S + + LL S +L + TPKW+ PL+L ID+YEK + RR L S C R WK++D + RW Y PA+NK IDDAF +GE R T RRKY IQF M+Q NEETGN RPIM + S D ++ ++ DV+ + E L + L++ V + +P+D D L+AVLRLCLRL++ E + FA LGG+ LL L + S F GI S+ TL++RHV+ED + L+ MEK+IR+ +S ++E++YI R PAACR +F AK ILR + NK+ E D+ T +K + K P+ L +++ VI +LLN L ++ A E +G + A KD + + + S + LLAEL++SYG AK+I +H Y G + + E+ SAL+F+LD LL DKD K L AALA+ + E A+V E+K +L+R+++ PES KH KIQAL+ LI+ ++ESCP+S T + ++ ++I++IM+KRGLITDLA+V +LDLSSPN++ T+NS LK LE +S+ +N P+ + + +++ D +++ AE SN V++ D E +A A D S + +A D+S + +++ +S + N+ DE+ +++ + D DN F P+ G+
Sbjct: 1374 LDILVDMGFSRELAGVALAHTANDVGQAAELLI----SHPHLDASSSSAPNAEVDM------TEEDQVVQAIAMSLD----------------ETDKPSEKGVLLPKPVTPPLTNEVPLGKSELDSFTGIILSGTLKLLDQLPDTVYKCCELLIAVAKRNGTVWFQNMIIELINEITVNISSLVRSSFIEGTDLEKSGDDWANHLSTLPEARKLSSRIHLLALLFNEMKSSCAGQISKSDLFERVVELLDRSAYLLTLVYEKEAKITTPKWLCPLVLLIDVYEKYAVASQRRKQLLSKNCKRQWKFFDDRTSRWTTYIPATNKAIDDAFRTGEQFYRFTAMRRKYCIQFNNMLQVNEETGNWRPIMFT-----SED-------------------------TEEDQGDVVDWVHI-ESLSSMFSKTLIETMVSLIKLPIDPDTLHAVLRLCLRLSRNHESSCLFAELGGITTLLNLPRGSQFVGIVSMTTLIIRHVIEDKEVLKVMMEKIIRSQANSSLINNREMHYIFRYFNPAACRDIDLFKESAKSILRV--NIFNKKLDEEDDRTPTFLKGISAKTGENPDGYI-ALPKLTCDVIYELLNLL--------------PIKHAEEFNAPEG--------------------------------EKGAKKDDECKLNENIFTTSTILALLAELIRSYGVIAKVICEHSYTRGFADAVSEECSALSFILDNLLYVPTVNPPNGDKDFPPYSKILFAALATVTYSSEVHQAIVTEIKSSLSRTVLCPESADKHLKIQALSSLITLVVESCPNS---TFNSIYNRSTTGSSSSILRIMVKRGLITDLAKVIQSLDLSSPNMSTTVNSLLKTLEFMSKSLNVPSNVYPTRLHRTRPDASNTTAGAEQQVSNEQV---MEVMINDASE--------------DAAVANNDSS---NIDANSSDISQSVAALNNSSSGD---IPPQNNAIADEIANEIFDLDAYDNEFNSHPRSGT 2252 HSP 3 Score: 264.233 bits (674), Expect = 1.787e-66 Identity = 124/251 (49.40%), Postives = 167/251 (66.53%), Query Frame = 0
Query: 29 QIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLP---NLSIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGS 276
Q K IKPLL +S+LGR+LAELF LLVKLCVGSPIR+R P T PSQ + ++ LN LL GLSW P + +P +RLTF+ CS+GF+ PMLFDD+K+PYHLML FV GG AFF F A+ + +S+ELPDG EFLD+WL+LLE+M NP+++L+SPH+LP + +F PFDP+++LIRTHK F ++++W ++ + S+L+IL H+LKGE II + L S
Sbjct: 1062 QAKYIKPLLTVASKLGRSLAELFGLLVKLCVGSPIRRRSHHTAPSTIQPPSQQSRNISKYLNVLLFHGLSWQQPFDTNVPKYRLTFYVCSIGFSSPMLFDDRKFPYHLMLQNFVVHGGHDAFFHCFKMAIGQNSDI-----WKSSELPDGIVEFLDAWLLLLEKMTNPQSLLDSPHSLPAKADAKNASFVPFDPIQFLIRTHKKTFTCILELWNDKLFQMNSENIINSLLTILSHLLKGESIIEKYLKDKS 1307
BLAST of EMLSAG00000004991 vs. nr
Match: gi|934158656|gb|KPM07940.1| (E3 ubiquitin-protein ligase HUWE1-like protein [Sarcoptes scabiei]) HSP 1 Score: 980.704 bits (2534), Expect = 0.000e+0 Identity = 541/1068 (50.66%), Postives = 710/1068 (66.48%), Query Frame = 0
Query: 1892 SILGDVEIPEGVDPSFLAALPEEMRQEVIDDQRRLQ---NIRQ--RAQQQNTEGSNV---PEVNPEFLAALPPNIQEEVLAQQRMEQQRQLSQ-VNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNNGGNSLSSILRGSV-SRL-GNQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKIN-TTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDIMASIQSSFQLPNK--SKRSSTATPVK-SASSQQQQQXKYPVIRRNSLLTDKLLRLLSLI--SIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHL-RLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANV----LDLQNELLSIKS--------TNFEHDRSGKDKGGLLDRFTKETVVLNAPSKQXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEENKMDVDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQ-----EQLADNKEMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHT 2924
+ILGD E+PEGVDPSFLAALPE +RQEVI +Q RLQ N+R A Q N SN EV+PEFLAALPPNIQEEVLAQQR EQQR +Q NP+APVDP F +LP +LR+ VL D++DSQ+ LP +LA EA+SLRR+ E RNR MHERFF N++S I+R + SR+ + + NL R +F + G + + ++TR L N FKG+ LLD +GL+ ++ILLF++++ IN +++ IL P + ++ + +E+ + + + +K ++ P++ S +Q P +++N +LTD+L+RL+ I S R ++ + S E ++ IS + L +L VDVL +K S EG+ D +AL++ L + R I+ + LL+G + LG +V + ++++ L+ IKS + FE ++ K K + DR++K T+++ + S + +E + P+ S + +++ I + + + +NSS+++ E + +E L LD LWE L +CL L+E PD H VLVLQ +VE+FFLVHA Q K + + E Q++HI E LA + +++ S SL +T+KFL FAE HR VLNQILRQS HL +GPFSVLVDHTR+LDFD+KRRYFR ELER+D+ +RR+DL VH+RRE++FE+S+REL RR+ + KNRFY+VFEGEEGQDAGGLLREWY IISREIFNP YALF TSPGD+VTYMI +S N NHL YFKFVGRVI+KAIYDNKLL+CYFTRSFYKHILGK VKYTDMESEDYAFY+GL FL+ H V++LG +LTFS EIQEFGVTEIRDL+P+GRNI VTEE K +Y++LVCQ KMTG+I+KQL +FLEGFY IIP+ LISIFNE ELELL+SGLPTIDIDDLK+NTEYHKYQ NSLQI WFWRALR+FDQ D+AKF+QFVTG+SKVPLQGF+ LEGMNG QKFQIH DDRSTDRLPSAHT
Sbjct: 2295 AILGDQELPEGVDPSFLAALPENIRQEVIAEQFRLQRLNNLRTLTTANQSNASSSNAVQNAEVSPEFLAALPPNIQEEVLAQQRAEQQRLNAQNTNPDAPVDPVSFLNSLPPTLRRQVLTDLDDSQVQLLPPDLANEARSLRRDYEMRNRQ-MHERFFT-TNNTISRIIRSAANSRVRYDPFNFQNL--RLSFAIRGGSYDRDNFQSLTRNVPRP--LTNR-SFKGKHLLDFDGLASVMILLFLNENVINKSSKTQSIL-------PITNHILSSRNNQIEQVLKYSLFFGEFIFHLEFLDNQITKVGQSSKPLELSPLTQLLNLLSSPFVKKNRVLTDRLIRLVGFIITSFIRDQPEGQRNQQPIDLSNEIKEKLKDIEQSISDERLLKLVVDVLITKPYSTEGISDSSALIVKLCNLFPSCRSILYKFLLEGVRNLGEIVYKEINTLIFEMKDLLIKIKSQKTIDTLDSTFE--KTIKGKSNIQDRYSKMTIIVKSQSNLKQITGKEVQ-------LPSMSNLISKSSSQFLFCRILKIILHIRENNAKAQQSQAAKNSSETSMEVDNEDKLCVE-----LQLDHLWEKLSECLTLLSEAPDDHVVLVLQPAVESFFLVHA---QEKSKNQTKANEPVNVQVSHINQAGQSEVLATDSNVDNESRQYG----SLPFETQKFLIFAEKHRVVLNQILRQSNVHLMNGPFSVLVDHTRILDFDVKRRYFRQELERLDQNIRRDDLPVHIRREHIFEDSYRELHRRTCNDWKNRFYVVFEGEEGQDAGGLLREWYTIISREIFNPNYALFTTSPGDRVTYMINSASHCNSNHLSYFKFVGRVISKAIYDNKLLDCYFTRSFYKHILGKAVKYTDMESEDYAFYQGLVFLLEHNVNELGTELTFSLEIQEFGVTEIRDLVPDGRNIIVTEENKHEYVKLVCQEKMTGSIKKQLHSFLEGFYEIIPKHLISIFNEHELELLISGLPTIDIDDLKANTEYHKYQINSLQIQWFWRALRTFDQADRAKFLQFVTGTSKVPLQGFSALEGMNGPQKFQIHLDDRSTDRLPSAHT 3327 HSP 2 Score: 378.252 bits (970), Expect = 3.634e-101 Identity = 285/905 (31.49%), Postives = 452/905 (49.94%), Query Frame = 0
Query: 382 EPAPGFSFYDLQILRDMGFSRERCLEAMDRTGA-MYEATEYLLTGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVVSFQVSSNTEGNNEEDSQQQSGKEEEENEGGSFGLSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSPIEEDVFRTTESNKAAV----RIHLFILLFEECKLLCAKIVEESSVVPIM-----------THLLTVSFDVLKRCSGSVGTPKWMTPLLLFIDLYEKVILGMNRRASL---TSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIK-SKGSRDAVVKSSATSSNSNNAMELGEEE-----KKVSDPNE--EDVITTIPTA-----EGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQTTSKEINYILRVLAPAACRSPSIFVSVAKDILRFE-PSPLNKRGETDEDTRLLVKSLPTKAPNSVAPPLGEVSQSVICDLLNFLVKTPDICESSNNATVVESATELKNNKGGGAPNVIRNMXTGDLPGVLXXPPVTITTAQPCDTSASKDMKKPFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSKA---DKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSS 1250
+PA +++L +MGF + L+A G + A +++L S N+ E ++D+++AIA+SL +D E D + + K +N L+ M+ L L L+D +PE V + C+L++A RNGKE+ E+I+ LI + + I+ L K + + S D ++K A+ RIHL ++L+ E K K + +V PI+ ++LL VSF+ ++ TPKW+ P +LFIDLYE + ++RR L T R WK+++ + RW Y P NK+IDD+F +GE RI RRKYTI FG+M+Q N+ET N RP+M+ I+ K + ++ +K+ + N E +E KK+ D E ++ T++ T+ + L + + +L+++ + IP++ + LNA+LRL LRLT+ +E A F GV LLL++ QSS+F G SL L++RHVLED +L+ TME++I NS +S E+NY +R +PAACR+ ++F AK++ RF P P N ++ + L S + + A + + S+ VI DLLN L + NA +ES C S++ K V +L EL +SY AKLI + Y G++E++ E SA++F+L++LL+ A DKD + L+A++AS PE L E+K AL +++ PES KH+KIQA+ LI+ M E S I + F ++ K+++K+ L D+A++ + +DLSSP I+ TIN+ LK LE LS+ N P L+ S
Sbjct: 1183 QPAQAPEHAGVRVLIEMGFPPDLALDAFFNVGDDVTAAADWILANHSLSLNSVNE-------------------EDDMIKAIALSL-LD----------SSGGESDEKIKKEKICHKNYHQLIPLTSDEMNRLMDNILAKILILIDQVPEIVNKCCELMVAFSNRNGKEWFEEIINNLIIDTITIIEQLNKKTFYKQDNDSGTTTDWSAELSTSKEAIQLSSRIHLLLVLYNEAK----KNFSQEAVNPIIIDQLISLLENSSYLLNVSFEK----QSTITTPKWIAPAVLFIDLYEHYAVALDRRNLLLAETMNAKRVWKYFEDRAQRWNSYQPDINKSIDDSFRNGELYKRINANRRKYTINFGSMVQINDETCNLRPVMLFIEPDKPTVNSTIKNEMNERDLNVEREKPNQENDFLSKKIDDEYESKKNSKTSVATSYKSIFKLLNSKQSKSLIESSINLFKIPIEPETLNAILRLNLRLTRNYENASYFVEKNGVNLLLKIPQSSVFCGFVSLVILIIRHVLEDEKSLQTTMERLICNFNQNS-NSSHEVNYQIRFYSPAACRNANLFKISAKNLFRFNVPVPSNSNRRSNSENTLRTDSKLSLDIWNNADKIPKPSKDVISDLLNMLPMKYAQQQQRKNAQ-LESG---------------------------------------CLNEKSEEKCKDVFHVHTVLSILDELTRSYHIVAKLICEFNYPIGLIESMVE-CSAISFILNKLLSHNANYGDKDCATYSRALLASIASVPFTPEVHHTLFNEIKSALKNTILLPESVEKHAKIQAMMTLIA-MFEMPSSLIHPYRANNFTN------SSFFKLLVKKNLAADVAKLINHMDLSSPMISNTINTLLKTLEILSKNHNYPNNLLGS 2000 HSP 3 Score: 166.392 bits (420), Expect = 6.350e-37 Identity = 76/135 (56.30%), Postives = 95/135 (70.37%), Query Frame = 0
Query: 27 HHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPA---APSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWAL 158
HH K IKPL+ +SRLGRALAELF LLVKL +G PIR RR H P P+ P+ PA ++ LN+LLA GLSW P + +P RLTFF CS+GF+ PMLFDD+K+PYHLML FV S GL++FF+ + A+
Sbjct: 984 HH--KCIKPLITVASRLGRALAELFGLLVKLSIGLPIRNRRNHHQPNFPSHFNLPTLPAQRISKHLNKLLARGLSWKPSFKTDVPKIRLTFFVCSIGFSSPMLFDDRKFPYHLMLKNFVLSNGLESFFECYETAI 1116
BLAST of EMLSAG00000004991 vs. nr
Match: gi|808127135|ref|XP_012166531.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1 isoform X2 [Bombus terrestris]) HSP 1 Score: 974.926 bits (2519), Expect = 0.000e+0 Identity = 500/809 (61.80%), Postives = 591/809 (73.05%), Query Frame = 0
Query: 2169 MASIQSSFQLPNKSKRSSTATPVKSASSQQQQQXKYPVIRRNSLLTDKLLRLLSLISIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNFEHDR----------SGKDKGGLLDRFTKETVVLNAPSK-QXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEENKMDVDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQ--EQLAHIQEQLADNK-------EMESSSTASSCSSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+A S+ L N S+ ++T T SA Q + VI+R+S LTDK+L+LLSLISIG + + + + + ++ P +HL+LAV VLTSKSCSEEGLED TALLLNLSH P PTR +IL+LLL+GA EL NVV ++ DL L+ +K N E R SG + LLDRFT E VV+ APSK + D+Q ++ S T +++ + +A+ N+ + +T++A + + K+ LSE L LD LWE L CL +L TPD HAVLVLQ +VEAFFLVH++S+ ++ ETR+ L + +DN+ + +S S +L PD KFL FAETHRTVLNQILRQ+T HLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFE+SFREL RR+ +E KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF SPGD+VTYMI SS NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHILG VK+TDMESEDY+FYKGL +L H ++DLGY+LTFSTE+ EFGV ++RDLIPNGRNI VTEETKL+YIRLVCQMKMTGAIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLP +DI+DLK+NTEYHKY NSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLRT LLKAIHECSE F
Sbjct: 3353 VARSHSNSNLGNDSEHNTT-TFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYRRQEGNKNKRTDVNKEQSVAAP--EEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDL---LMELKVLNRELRRNSIEEQPSTSSGGSRWVLLDRFTNENVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRIL----KVIVQIREAVRLANNKKTSNQSSESSVDTGNTNQASESGEDKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTNTETREVVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTSQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIVVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGF 4151 HSP 2 Score: 303.138 bits (775), Expect = 2.550e-78 Identity = 149/277 (53.79%), Postives = 183/277 (66.06%), Query Frame = 0
Query: 28 HQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSIG---NFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSK------GSKGEDDKNSG---GGAMPSR 292
H+I K LL +S++GRALAELFA+LVKLC+GSPIRQRRGQ VPP PA PSQ + +VA+ALN +L GLSW P S +P ++TF C++ F P LFD+K YP+HLML KFV GG AFF F+W LS GG+ EGLE LPD TGEFLD WLM+LE+MVNPK +L+SP+ + + G ++ FDP+KYL HK AF AVM +WGK PLK YG M E +LSIL HIL+ E+II E K G SG GGA+ R
Sbjct: 1118 HRITYPKHLLGLTSKMGRALAELFAILVKLCMGSPIRQRRGQQVPPIPALPSQASRSVATALNCVLIRGLSWDKLPPSTVPKLQVTFLMCTLSFVSPALFDEKGYPFHLMLQKFVKLGGQNAFFAAFHWVLSGGGQFPLSEGLEHPNLPDCTGEFLDGWLMILEKMVNPKTLLDSPYVVSSKCTGIYKVYETFDPIKYLTDIHKEAFEAVMLMWGKKPLKNYGVHMTELILSILRHILRSEQIIKEHAEKEENSEAAISGSTSGTSGIGRGGAISDR 1394 HSP 3 Score: 284.648 bits (727), Expect = 9.465e-73 Identity = 157/288 (54.51%), Postives = 191/288 (66.32%), Query Frame = 0
Query: 1899 IPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVP-EVNPEFLAALPPNIQEEVLAQQRMEQQRQLS-QVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNN---GGNSLSSILRGSVSRLG--NQYIIGNLVGREAFRLS-GSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERAN-ETKDIMASIQSSFQ 2177
+P+GVDPSFLAALPE+MR EVI +Q RLQ IRQRAQ E P EVNPEFLAALPP IQEEVLAQQR+EQQR + NPE PVD FFQ L SLRQ++L DME+SQ++ LP +LA EAQ+LRRE E RNR+ M +RF N+ G +LSSILR S G +Y I + R S G T A +R +GRQLLD+E ++C+L+LLFVD+ KINT RLHR++RNLCYH TR+WVVR L+SI+ER+N E KDI F+
Sbjct: 2931 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQGNAALSSILRNSGRGRGGGTRYAIHTVAQRGWQPWSHGDPITHHGAG---------------LRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSNDENKDIAMDFGGKFR 3203
BLAST of EMLSAG00000004991 vs. nr
Match: gi|815906178|ref|XP_012238909.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1 isoform X2 [Bombus impatiens]) HSP 1 Score: 973.77 bits (2516), Expect = 0.000e+0 Identity = 500/809 (61.80%), Postives = 590/809 (72.93%), Query Frame = 0
Query: 2169 MASIQSSFQLPNKSKRSSTATPVKSASSQQQQQXKYPVIRRNSLLTDKLLRLLSLISIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNFEHDR----------SGKDKGGLLDRFTKETVVLNAPSK-QXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEENKMDVDENSSDSTSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQ--EQLAHIQEQLADNKEMESSSTASSCS-------SNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+A S+ L N S+ ++T T SA Q + VI+R+S LTDK+L+LLSLISIG + + + + + ++ P +HL+LAV VLTSKSCSEEGLED TALLLNLSH P PTR +IL+LLL+GA EL NVV ++ DL L+ +K N E R SG + LLDRFT E VV+ APSK + D+Q ++ S T +++ + +A+ N+ + +T++A + + K+ LSE L LD LWE L CL +L TPD HAVLVLQ +VEAFFLVH++S+ ++ ETR+ L + +DN+ S + S +L PD KFL FAETHRTVLNQILRQ+T HLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFE+SFREL RR+ +E KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF SPGD+VTYMI SS NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHILG VK+TDMESEDY+FYKGL +L H ++DLGY+LTFSTE+ EFGV ++RDLIPNGRNI VTEETKL+YIRLVCQMKMTGAIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLP +DI+DLK+NTEYHKY NSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLRT LLKAIHECSE F
Sbjct: 3353 VARSHSNSNLGNDSEHNTT-TFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYRRQEGNKNKRTDVNKEQSVAAP--EEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDL---LMELKILNRELRRNSIEEQPSTSSGGSRWVLLDRFTNENVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRIL----KVIVQIREAVRLANNKKTSNQSSESSVDTGNTNQASESGEDKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSISRDRHLSTNTETREVVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPPPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIVVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGF 4151 HSP 2 Score: 303.908 bits (777), Expect = 1.477e-78 Identity = 145/264 (54.92%), Postives = 182/264 (68.94%), Query Frame = 0
Query: 28 HQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSIG---NFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKGEDDKNSGGGA 288
H+I K LL +S++GRALAELFA+LVKLC+GSPIRQRRGQ VPP PA PSQ + +VA+ALN +L GLSW P S +P ++TF C++ F P LFD+K YP+HLML KFV GG AFF F+W LS GG+ EGLE LPD TGEFLD WLM+LE+MVNPK +L+SP+ + + G ++ FDP+KYL HK AF AVM +WGK PLK YG M E +LSIL HIL+ E+II E K E+ + + GG+
Sbjct: 1118 HRITYPKHLLGLTSKMGRALAELFAILVKLCMGSPIRQRRGQQVPPIPALPSQASRSVATALNCVLIRGLSWDKLPPSTVPKLQVTFLMCTLSFVSPALFDEKGYPFHLMLQKFVKLGGQNAFFAAFHWVLSGGGQFPLSEGLEHPNLPDCTGEFLDGWLMILEKMVNPKTLLDSPYVVSSKCTGIYKVYETFDPIKYLTDIHKEAFEAVMLMWGKKPLKNYGVHMTELILSILRHILRSEQIIKE---HAEKEENSEGAIGGS 1378 HSP 3 Score: 285.804 bits (730), Expect = 4.597e-73 Identity = 158/287 (55.05%), Postives = 194/287 (67.60%), Query Frame = 0
Query: 1899 IPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVP-EVNPEFLAALPPNIQEEVLAQQRMEQQRQLS-QVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNN---GGNSLSSILRGSVSRLG--NQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERAN-ETKDIMASIQSSFQ 2177
+P+GVDPSFLAALPE+MR EVI +Q RLQ IRQRAQ E P EVNPEFLAALPP IQEEVLAQQR+EQQR + NPE PVD FFQ L SLRQ++L DME+SQ++ LP +LA EAQ+LRRE E RNR+ M +RF N+ G +LSSILR S G +Y I + R G + +IT H A +R +GRQLLD+E ++C+L+LLFVD+ KINT RLHR++RNLCYH TR+WVVR L+SI+ER+N E KDI F+
Sbjct: 2930 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQGNAALSSILRNSGRGRGGGTRYAIHTVAQR------GWQPWSHGDPSITH---HGA----GLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSNDENKDIAMDFGGKFR 3203
BLAST of EMLSAG00000004991 vs. nr
Match: gi|820863765|ref|XP_012348525.1| (PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase HUWE1 [Apis florea]) HSP 1 Score: 969.533 bits (2505), Expect = 0.000e+0 Identity = 500/811 (61.65%), Postives = 594/811 (73.24%), Query Frame = 0
Query: 2169 MASIQSSFQLPNKSKRSSTATPVKSASSQQQQQXKYPVIRRNSLLTDKLLRLLSLISIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNFEHDR----------SGKDKGGLLDRFTKETVVLNAPSK-QXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEENKMDVDENSSDS--TSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQ--EQLAHIQEQLADNKEMESSSTASSCS-------SNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+A S+ L N ++ ++T T SA Q + VI+R+S LTDK+L+LLSLISIG + + + + + ++ P +HL+LAV VLTSKSCSEEGLED TALLLNLSH P PTR +IL+LLL+GA EL NVV ++ DL L+ +K N E R +G + LLDRFT E+VV+ APSK + D+Q ++ S T +++ + +A+ N++ E+S D+ T++A + + K+ LSE L LD LWE L CL +L TPD HAVLVLQ +VEAFFLVH++S+ ++ E R+ L + +DN+ S + S +L PD KFL FAETHRTVLNQILRQ+T HLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFE+SFREL RR+ +E KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF SPGD+VTYMI SS NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHILG VK+TDMESEDY+FYKGL +L H ++DLGY+LTFSTE+ EFGV ++RDLIPNGRNI VTEETKL+YIRLVCQMKMTGAIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLP +DI+DLK+NTEYHKY NSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLRT LLKAIHECSE F
Sbjct: 3350 VARSHSNSNLGNDTEHNTT-TFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYRRQEGNKNKRTDMNKEQSIAAP--EEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDL---LMELKVLNRELRRNSIEEQPSTSTGGSRWVLLDRFTNESVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRIL----KVIVQIREAVRLAN--NKKTSTQSSESSVDTENTNQASESGEDKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTSTENREVVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIIVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGF 4148 HSP 2 Score: 301.982 bits (772), Expect = 5.475e-78 Identity = 143/262 (54.58%), Postives = 180/262 (68.70%), Query Frame = 0
Query: 28 HQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSIG---NFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKGEDDKNSGG 286
H++ K LL +S++GRALAELFA+LVKLC+GSPIRQRRGQ +PP PA PSQ + +VA+ALN +L GLSW P S +P ++TF C++ F P LFD+K YP+HLML KFV GG AFF F+W LS GG+ EGLE LPD TGEFLD WLM+LE+MVNPK +L+SP+ + + G ++ FDP+KYL HK AF AVM +WGK PLK YG M E +LSIL HIL+ E+II E K ++ N GG
Sbjct: 1118 HRLTYPKHLLGLTSKMGRALAELFAILVKLCMGSPIRQRRGQQMPPIPALPSQASRSVATALNCVLIRGLSWDKLPPSTVPKLQVTFLMCTLSFVSPALFDEKGYPFHLMLQKFVKLGGQNAFFAAFHWVLSGGGQFPLSEGLEHPNLPDCTGEFLDGWLMILEKMVNPKTLLDSPYVVSSKCTGIYKVYETFDPIKYLTDIHKEAFEAVMLMWGKKPLKNYGVHMTELILSILRHILRSEQIIKEHAEK-----EENNEGG 1374 HSP 3 Score: 281.952 bits (720), Expect = 6.807e-72 Identity = 156/278 (56.12%), Postives = 191/278 (68.71%), Query Frame = 0
Query: 1899 IPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVP-EVNPEFLAALPPNIQEEVLAQQRMEQQRQLS-QVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNN---GGNSLSSILRGSVSRLG--NQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERAN-ETKDI 2168
+P+GVDPSFLAALPE+MR EVI +Q RLQ IRQRAQ E P EVNPEFLAALPP IQEEVLAQQR+EQQR + NPE PVD FFQ L SLRQ++L DME+SQ++ LP +LA EAQ+LRRE E RNR+ M +RF N+ G +LSSILR S G +Y I + R G + +IT H A +R +GRQLLD+E ++C+L+LLFVD+ KINT RLHR++RNLCYH TR+WVVR L+SI+ER+N E DI
Sbjct: 2927 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQGNAALSSILRNSGRGRGGGTRYAIHTVAQR------GWQPWTNGDPSITH---HGA----GLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSNDENXDI 3191
BLAST of EMLSAG00000004991 vs. nr
Match: gi|1035587454|ref|XP_016910514.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1 isoform X2 [Apis cerana]) HSP 1 Score: 969.148 bits (2504), Expect = 0.000e+0 Identity = 500/811 (61.65%), Postives = 594/811 (73.24%), Query Frame = 0
Query: 2169 MASIQSSFQLPNKSKRSSTATPVKSASSQQQQQXKYPVIRRNSLLTDKLLRLLSLISIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNFEHDR----------SGKDKGGLLDRFTKETVVLNAPSK-QXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEENKMDVDENSSDS--TSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQ--EQLAHIQEQLADNKEMESSSTASSCS-------SNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+A S+ L N ++ ++T T SA Q + VI+R+S LTDK+L+LLSLISIG + + + + + ++ P +HL+LAV VLTSKSCSEEGLED TALLLNLSH P PTR +IL+LLL+GA EL NVV ++ DL L+ +K N E R +G + LLDRFT E+VV+ APSK + D+Q ++ S T +++ + +A+ N++ E+S D+ T++A + + K+ LSE L LD LWE L CL +L TPD HAVLVLQ +VEAFFLVH++S+ ++ E R+ L + +DN+ S + S +L PD KFL FAETHRTVLNQILRQ+T HLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFE+SFREL RR+ +E KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF SPGD+VTYMI SS NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHILG VK+TDMESEDY+FYKGL +L H ++DLGY+LTFSTE+ EFGV ++RDLIPNGRNI VTEETKL+YIRLVCQMKMTGAIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLP +DI+DLK+NTEYHKY NSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLRT LLKAIHECSE F
Sbjct: 3349 VARSHSNSNLGNDTEHNTT-TFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYRRQEGNKNKRTDVNKEQSIAAP--EEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDL---LMELKVLNRELRRNSIEEQPSTSTGGSRWVLLDRFTNESVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRIL----KVIVQIREAVRLAN--NKKTSTQSSESSVDTENTNQASESGEDKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTSTENREVVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIIVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGF 4147 HSP 2 Score: 301.982 bits (772), Expect = 6.421e-78 Identity = 143/262 (54.58%), Postives = 180/262 (68.70%), Query Frame = 0
Query: 28 HQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSIG---NFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKGEDDKNSGG 286
H++ K LL +S++GRALAELFA+LVKLC+GSPIRQRRGQ +PP PA PSQ + +VA+ALN +L GLSW P S +P ++TF C++ F P LFD+K YP+HLML KFV GG AFF F+W LS GG+ EGLE LPD TGEFLD WLM+LE+MVNPK +L+SP+ + + G ++ FDP+KYL HK AF AVM +WGK PLK YG M E +LSIL HIL+ E+II E K ++ N GG
Sbjct: 1118 HRLTYPKHLLGLTSKMGRALAELFAILVKLCMGSPIRQRRGQQMPPIPALPSQASRSVATALNCVLIRGLSWDKLPPSTVPKLQVTFLMCTLSFVSPALFDEKGYPFHLMLQKFVKLGGQNAFFAAFHWVLSGGGQFPLSEGLEHPNLPDCTGEFLDGWLMILEKMVNPKTLLDSPYVVSSKCTGIYKVYETFDPIKYLTDIHKEAFEAVMLMWGKKPLKNYGVHMTELILSILRHILRSEQIIKEHAEK-----EENNEGG 1374 HSP 3 Score: 284.263 bits (726), Expect = 1.416e-72 Identity = 157/278 (56.47%), Postives = 192/278 (69.06%), Query Frame = 0
Query: 1899 IPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVP-EVNPEFLAALPPNIQEEVLAQQRMEQQRQLS-QVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNN---GGNSLSSILRGSVSRLG--NQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERAN-ETKDI 2168
+P+GVDPSFLAALPE+MR EVI +Q RLQ IRQRAQ E P EVNPEFLAALPP IQEEVLAQQR+EQQR + NPE PVD FFQ L SLRQ++L DME+SQ++ LP +LA EAQ+LRRE E RNR+ M +RF N+ G +LSSILR S G +Y I + R G + +IT H A +R +GRQLLD+E ++C+L+LLFVD+ KINT RLHR++RNLCYH TR+WVVR L+SI+ER+N E KDI
Sbjct: 2926 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQGNAALSSILRNSGRGRGGGTRYAIHTVAQR------GWQPWSNGDPSITH---HGA----GLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSNDENKDI 3190
BLAST of EMLSAG00000004991 vs. nr
Match: gi|1035587452|ref|XP_016910505.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1 isoform X1 [Apis cerana]) HSP 1 Score: 968.763 bits (2503), Expect = 0.000e+0 Identity = 500/811 (61.65%), Postives = 594/811 (73.24%), Query Frame = 0
Query: 2169 MASIQSSFQLPNKSKRSSTATPVKSASSQQQQQXKYPVIRRNSLLTDKLLRLLSLISIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNFEHDR----------SGKDKGGLLDRFTKETVVLNAPSK-QXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEENKMDVDENSSDS--TSEAKANSQWKIESLSEQLSLDSLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQ--EQLAHIQEQLADNKEMESSSTASSCS-------SNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+A S+ L N ++ ++T T SA Q + VI+R+S LTDK+L+LLSLISIG + + + + + ++ P +HL+LAV VLTSKSCSEEGLED TALLLNLSH P PTR +IL+LLL+GA EL NVV ++ DL L+ +K N E R +G + LLDRFT E+VV+ APSK + D+Q ++ S T +++ + +A+ N++ E+S D+ T++A + + K+ LSE L LD LWE L CL +L TPD HAVLVLQ +VEAFFLVH++S+ ++ E R+ L + +DN+ S + S +L PD KFL FAETHRTVLNQILRQ+T HLADGPFSVLVDHTRVLDFD+KRRYFRTELERMDE +RRE+LAVHVRR NVFE+SFREL RR+ +E KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALF SPGD+VTYMI SS NPNHL Y+KFVGRVIAKAIYDNKLLECYFTRSFYKHILG VK+TDMESEDY+FYKGL +L H ++DLGY+LTFSTE+ EFGV ++RDLIPNGRNI VTEETKL+YIRLVCQMKMTGAIRKQL+AFLEGFY+IIP+RLISIFNEQELELL+SGLP +DI+DLK+NTEYHKY NSLQI WFWRALR FDQ D+AKF+QFVTG+SKVPLQGFA LEGMNG+QKFQIHR+DRSTDRLPSAHTCFNQLDLP YETYDKLRT LLKAIHECSE F
Sbjct: 3354 VARSHSNSNLGNDTEHNTT-TFESSAFGQLISMLNWSVIKRSSQLTDKVLKLLSLISIGLTEVNPYRRQEGNKNKRTDVNKEQSIAAP--EEHLKLAVQVLTSKSCSEEGLEDATALLLNLSHCPDPTRQLILKLLLEGAMELANVVCEHIKDL---LMELKVLNRELRRNSIEEQPSTSTGGSRWVLLDRFTNESVVITAPSKVKAGSDLQLPSMAALVSKTSSQAFFLRIL----KVIVQIREAVRLAN--NKKTSTQSSESSVDTENTNQASESGEDKLPLLSESLVLDHLWETLSACLLELEYTPDQHAVLVLQPAVEAFFLVHSSSSTSRDRHLSTSTENREVVPDLTPVSPIYSDNEGTSQSEATINTSWDIQPTPQKTLPPDQLKFLKFAETHRTVLNQILRQTTTHLADGPFSVLVDHTRVLDFDVKRRYFRTELERMDEGIRREELAVHVRRNNVFEDSFRELHRRNADEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFTVSPGDRVTYMINSSSHCNPNHLCYYKFVGRVIAKAIYDNKLLECYFTRSFYKHILGILVKHTDMESEDYSFYKGLVYLTEHNIADLGYELTFSTEVNEFGVNDVRDLIPNGRNIIVTEETKLEYIRLVCQMKMTGAIRKQLNAFLEGFYDIIPKRLISIFNEQELELLISGLPNVDIEDLKANTEYHKYNANSLQIQWFWRALRGFDQADRAKFLQFVTGTSKVPLQGFAALEGMNGIQKFQIHREDRSTDRLPSAHTCFNQLDLPVYETYDKLRTNLLKAIHECSEGFGF 4152 HSP 2 Score: 301.982 bits (772), Expect = 6.372e-78 Identity = 143/262 (54.58%), Postives = 180/262 (68.70%), Query Frame = 0
Query: 28 HQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSIG---NFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKGEDDKNSGG 286
H++ K LL +S++GRALAELFA+LVKLC+GSPIRQRRGQ +PP PA PSQ + +VA+ALN +L GLSW P S +P ++TF C++ F P LFD+K YP+HLML KFV GG AFF F+W LS GG+ EGLE LPD TGEFLD WLM+LE+MVNPK +L+SP+ + + G ++ FDP+KYL HK AF AVM +WGK PLK YG M E +LSIL HIL+ E+II E K ++ N GG
Sbjct: 1118 HRLTYPKHLLGLTSKMGRALAELFAILVKLCMGSPIRQRRGQQMPPIPALPSQASRSVATALNCVLIRGLSWDKLPPSTVPKLQVTFLMCTLSFVSPALFDEKGYPFHLMLQKFVKLGGQNAFFAAFHWVLSGGGQFPLSEGLEHPNLPDCTGEFLDGWLMILEKMVNPKTLLDSPYVVSSKCTGIYKVYETFDPIKYLTDIHKEAFEAVMLMWGKKPLKNYGVHMTELILSILRHILRSEQIIKEHAEK-----EENNEGG 1374 HSP 3 Score: 284.263 bits (726), Expect = 1.477e-72 Identity = 157/278 (56.47%), Postives = 192/278 (69.06%), Query Frame = 0
Query: 1899 IPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVP-EVNPEFLAALPPNIQEEVLAQQRMEQQRQLS-QVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNN---GGNSLSSILRGSVSRLG--NQYIIGNLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERAN-ETKDI 2168
+P+GVDPSFLAALPE+MR EVI +Q RLQ IRQRAQ E P EVNPEFLAALPP IQEEVLAQQR+EQQR + NPE PVD FFQ L SLRQ++L DME+SQ++ LP +LA EAQ+LRRE E RNR+ M +RF N+ G +LSSILR S G +Y I + R G + +IT H A +R +GRQLLD+E ++C+L+LLFVD+ KINT RLHR++RNLCYH TR+WVVR L+SI+ER+N E KDI
Sbjct: 2931 LPDGVDPSFLAALPEDMRDEVIAEQLRLQRIRQRAQATVVEELTGPVEVNPEFLAALPPAIQEEVLAQQRLEQQRHAAASANPEDPVDAAAFFQNLQPSLRQAILTDMEESQISVLPPDLAQEAQNLRREWEARNRHIMQDRFLNHVSQGNAALSSILRNSGRGRGGGTRYAIHTVAQR------GWQPWSNGDPSITH---HGA----GLRLRGRQLLDHESMACVLVLLFVDEPKINTLRLHRVIRNLCYHGSTREWVVRALLSIMERSNDENKDI 3195
BLAST of EMLSAG00000004991 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold450_size166944-snap-gene-0.20 (protein:Tk00123 transcript:maker-scaffold450_size166944-snap-gene-0.20-mRNA-1 annotation:"hypothetical protein DAPPUDRAFT_313219") HSP 1 Score: 1320.84 bits (3417), Expect = 0.000e+0 Identity = 761/1516 (50.20%), Postives = 970/1516 (63.98%), Query Frame = 0
Query: 1 MDIDSSSSTSATTIE----KKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGSKGEDDKNSGGGAMPSRVASRFANSFSNSPPFQMSLPDFLGDSFLSPVINNEAGGGXAAQGGEQPQXVQQPEQPPTLQPEQQQQSXSQNAPP--VITPXATAENNDDVEPAPGFSFYDLQILRDMGFSRERCLEAMDRTGAMYEATEYLL---------TGTQSSSNNEGESSGAQNTAAALRASPLSIEQEDLMRAIAMSLDVDVV-----SFQVSSNTEGNNEEDSQQQSGK----EEEENEGGSFG-LSKSVMDEFTQQALPGCLNLVDTLPETVFRTCDLLLAVFGRNGKEFKEKILRGLITEVKSAIDVLLDKLLELEKSQSPIEEDVFRTTESNKAAVRIHLFILLFEECKLLCAKIVEESSVVPIMTHLLTVSFDVLK-----RCSGSVGTPKWMTPLLLFIDLYEKVILGMNRRASLTSVCSRCWKWYDISSGRWCQYTPASNKTIDDAFWSGETSVRITTGRRKYTIQFGTMIQANEETGNRRPIMISIKSKGSRDAVVKSSATSSNSNN------AMELGEEEKKVSD------PNEEDVITTIPTAEGLKADEKSALLKACVGFVSIPVDSDALNAVLRLCLRLTQEFEQAVEFARLGGVKLLLELNQSSLFSGITSLATLLVRHVLEDPDTLRHTMEKVIRTSTLNSQT-TSKEINYILRVLAPAACRSPSIFVSVAKDILRFEPSPLNKR-GETDEDTRLLVKSLPTK--APNSVAPPLGEVSQSVICDLLNFLVK--------------TPDICESSNNATVVESAT-------ELKNNKGGGAPNVIRNMXTGDL--PGV-LXXPPVTITTAQ------PCDTSASKDMKK-PFLSKSAVCRLLAELVKSYGGCAKLITDHVYESGIVENIKEDTSALAFLLDELLTSKADKDIGQLVKTLIAALASCNHHPEAQSALVWEVKLALNRSLIQPESPVKHSKIQALTCLISTMIESCPSSIQQTQQPAFKQNLQCQMNNIVKIMLKRGLITDLARVPHALDLSSPNIAVTINSALKPLETLSRIVNQPTALVSSARKSKNDVPDSSAEDSTSNNPTGSGSSVLLGDIPEXEPNVNEGTNTTNSEATRAQGDESVEPDAEATEHDVSTTAESIDPNSESQLQTVEDTNDEDYDEMMDQLLERDGSDNFTIIPQV--GSNVNSDQDHH------QDGSRF---AGHGCQDDVDRAXESSQXD----AAIEEETEDLIEEEEDDDG 1424
M++D S S++ + +K +K + LH QIKQIKPLL SSRLGRALAELFALLVKLCVGSP+RQRRGQ P TPA PS PA AVAS+L +LLA+GLSW PP TSPIP FRLTFF CSVGFT PMLFD+KK+PYHLMLMKF+ SGG KAFFDTFYWALSCG ++PE+GLE +LPDGTGEFLDSWL+LLE+MVNPK +LESPH LP+ F+PFDP+KY+ RTH++AF AVM +WGK PLKVYGPRM+ESVLSILCHILKGEKII EK K S S ++ S + + F ++ N PP + S V + A AA GG S + A P + + P D+ R+ + E++D +AT+YLL SS GES G +A ++ +Q++LMRAIAMSL +V+ S G E Q S K ++EE F L KSV+D F AL GCL+L+DTLP+ VF+ CDLL+AVF RNG + KE L+ L EV ++ L D + EKS + + + S +AA RIHLF LL+E+CK+LCA++V+ S V+ MT LL V+K + + TPKWMTP++LFIDL+EKVILG+NRRA L VC+R WK+YDI+SG+WC Y A+NK+IDDAFW+GETS++I GRRKYTIQFG+M+Q NEETGNRRP+MI IK + + +AT ++ ++ M+ E ++ ++ PN E+V++TI +GL +EK LL++CV + IPVD +ALNAVLRLCLRLTQ+F+ A+ F +LGG+K+LL L Q S F G SLATLLVRHV+EDPDTL+HTMEKVIRT T N+ T +K+++Y+ R LAPAACR+P F+ VAKD+LR + + LNKR GE ++D RL++K+LP + AP S+ L EVS SVI DLLNFLV+ D+ +N + T + NN +IRN +GDL P P TT++ P T A +D+KK P L+KSA+CRLLAELVKSYGGCAKLITDHVYESGI E I E+T ALA++LDELL +K+ KD+G +VKTL+AA+ASCN+ EAQ+ LV EVK AL R+L PES +KH+KIQALT L+STMIESCP++ Q PAFK N Q MNNIVKIMLK+G+I DLAR+ H LDLSSPN+A T+N+ALKPLE L+RIVNQP L K + SA D++ SG++V GTNTTNSEATRAQGDE+VEPDAEATE D+ST AES+DPNSESQLQTVE+ NDE++DEMMDQ+LER+G N I+ +V S + D H QD S+F + QD+ DR E+S + A EE D++E+E+ + G
Sbjct: 1137 MEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPTPGFKPFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKDSIPAPSTASKIPSLTSSLRNPFMPAY-NLPPTSQPGGGGTSGAATSSVATSIA----AATGG----------------------SNTSGAGPSGLAAAPLPQLPPPHINPDHLVRLMDMGFPRERCIDAIQSTESLD------QATDYLLNDPFPTTPNVSVGGSSGAPGESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFAPLEKSVLDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDH-AKSEKSFENSMDHLITSPASAQAAARIHLFTLLYEDCKVLCARMVDSSKVITSMTTLLDTFQKVIKGLKDGASTVQIETPKWMTPMVLFIDLHEKVILGINRRAKLKDVCTRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRPKPISSEGETATKTDEDDNSPTPTPMDQSESARESNETVGEPNPNGEEVLSTI---DGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNNKDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRGGELEDDHRLILKALPPRAGAPTSMG-SLNEVSHSVIADLLNFLVQPEVESDAAIDSAEANADVNVGGSNVPPTPAVTPGSVSNVSVTNNATNRTQVIIRNSSSGDLQVPATDQAIPSENPTTSKEPKEDNPASTKAEEDLKKRPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELLINKSGKDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAAQAIQQMPAFK-NQQHTMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQPGTLAQRPGPGKAKSSEGSAPDASIAENIDSGTNV--------------GTNTTNSEATRAQGDETVEPDAEATELDISTAAESMDPNSESQLQTVEEGNDEEFDEMMDQILEREG--NPEILAEVIEHSIIEGDDTQHDSQMITQDESQFDQNVPNMSQDEEDRESENSTDEEPNSEAATEENGDIMEDEDQNGG 2597 HSP 2 Score: 201.445 bits (511), Expect = 2.453e-51 Identity = 106/198 (53.54%), Postives = 145/198 (73.23%), Query Frame = 0
Query: 1435 SGVSTDGGANSANHVTPSHPLLMGRQNLETGTGNARNS--ASTRSLPRTRGNSLRPYFQLNSR-AQNNPSTPAILQNFLGGSNPQELITFGFRRSGAPLMMDVVGLSLLDTMDNDMPDLD-SATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIENTRDE-------VRRKKEEERKIIKKL 1621
+G + GG NH+TP+HPLLMGRQ G+GN NS TR++ R R ++LR Y LN+R AQ NPS PAILQ+FLG +NPQ+LI G RR G PL++D G ++LD+++N++PD+D + G+GGR ALSSIPSAL+RW++ESRVIDG+SMHDCVT LKP+I EV+E RD+ +R+K++EE + ++KL
Sbjct: 2702 AGAAGPGGVT--NHMTPAHPLLMGRQ---AGSGNDSNSNRTGTRAVQRHRASNLRGYIHLNTRGAQGNPSAPAILQSFLG-TNPQDLINQGLRR-GTPLLVDF-GFAILDSLENELPDMDGNGMFGNGGRVALSSIPSALVRWSDESRVIDGDSMHDCVTALKPDITEVVETVRDQELTERRALRKKEQEEEESVQKL 2891
BLAST of EMLSAG00000004991 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold450_size166944-processed-gene-0.5 (protein:Tk00124 transcript:snap_masked-scaffold450_size166944-processed-gene-0.5-mRNA-1 annotation:"e3 ubiquitin-protein ligase huwe1-like isoform x5") HSP 1 Score: 1084.32 bits (2803), Expect = 0.000e+0 Identity = 550/814 (67.57%), Postives = 622/814 (76.41%), Query Frame = 0
Query: 2204 YPVIRRNSLLTDKLLRLLSLISIGRPDSLNNKDDKKGTGSKEESGSGNNLPPPISADHLRLAVDVLTSKSCSEEGLEDVTALLLNLSHGPVPTRDIILQLLLKGAQELGNVVRANVLDLQNELLSIKSTNFEHDRS-------------GKDKGGLLDRFTKETVVLNAPSK-----------------------------QXKKDMQEAKKSSPPSDTPAESTTTNTTTAPASTTTSTSDTTTIADQTNEEN-KMDVDENSSDSTSEAKANSQWKIESLSEQLSLD---------------SLWEALGDCLKDLAETPDHHAVLVLQSSVEAFFLVHAASTQPDEKKKVQQKETRQEQLAHIQEQLADNKEMESSSTASSC--SSNSLSPDTKKFLAFAETHRTVLNQILRQSTAHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECSEVMEF 2957
+ VIRR+S+LTDKLLRLLSLIS+G+PD + DK+ E S S N I D L+LAV+VLTSK+CSEEGLEDV ALLLNLS+GP PTRD IL+LLL+GAQELGNVVR NVL LQ EL +K+ + S KG +DRFT+++V+L AP+K + ++EA S A+ + N S T + D E++ KM+ D++ ++ ++ + + + S + SLD LW+AL CLKDLA+TPDHHAVLVLQ++VEAFFLVHAA TQP+EKKK +KETRQEQLAHIQEQL K + ++ S S SL+ DT KFL FA+THRTVLNQILRQST HLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDE +RREDLAVHVRRENVFE+SFREL RRS EE KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIY+ SQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHIL KPVKYTDMESEDY+FYKGLEFL+ H VSDLGY+LTFSTEI+EFGVTE+RDLIPNGRNI V+E+TK++Y+RLVCQMKMT AIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLP +D+DDLK+N+EYHKYQ NSLQI+WFWRALRSFDQTDKAKFMQFVTGSSKVPLQGF+ LEGMNG QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLR+YLLKAI ECSE F
Sbjct: 599 FSVIRRSSVLTDKLLRLLSLISVGQPDMKKAEQDKRAANEGEPSSS-NQAQEAIPGDLLKLAVEVLTSKACSEEGLEDVNALLLNLSYGPEPTRDTILKLLLQGAQELGNVVRQNVLQLQTELRELKAEQVKSGASEAVTPGDNQLESGSAKKGSFVDRFTQQSVILTAPTKVKGGSELQLPSMNSLTTKTSSQAFFLRVLKVIIQLREAALLSIKKRRAAKQSEKNAKKV-VSQALKTEEPKEGEDSVKEDDEKMETDQDEAEGVAKTEGDQPEQAASSESEESLDILESLESLSEELELQHLWDALSGCLKDLADTPDHHAVLVLQATVEAFFLVHAAPTQPEEKKKTLEKETRQEQLAHIQEQLEPPKSEDHAAAGPSSLNPSTSLTKDTDKFLDFADTHRTVLNQILRQSTTHLADGPFSVLVDHTRVLDFDIKRRYFRTELERMDENMRREDLAVHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALEGMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGF 1410 HSP 2 Score: 361.303 bits (926), Expect = 6.063e-102 Identity = 205/359 (57.10%), Postives = 250/359 (69.64%), Query Frame = 0
Query: 1867 GGXSXIASSNPIPSTSGTSQNPDYSSILG-DV-EIPEGVDPSFLAALPEEMRQEVIDDQRRLQNIRQRAQQQNTEGSNVPEVNPEFLAALPPNIQEEVLAQQRMEQQRQLS-QVNPEAPVDPGEFFQTLPASLRQSVLADMEDSQMAALPAELAAEAQSLRREXEQRNRNFMHERFFNN---GGNS-LSSILRGSVSRLGNQYIIG---NLVGREAFRLSGSRFTEASAAAITRERRHQAFLANSIRFKGRQLLDNEGLSCLLILLFVDDSKINTTRLHRILRNLCYHAPTRDWVVRCLVSILERANETKDI-------MASIQSSFQLPNKSKRSS----------TATPVKSASSQQ 2198
G AS PST DY++ILG D+ E+PEGVDPSFLAALPE+MRQEVID+QRRLQ++RQRA + E + V EVNPEFLAALPPNIQEEVL QQRMEQQRQ + NPEAPVDPGEF QTLP SLRQSVLADME+SQM+ALPA+LAAEAQ+LRRE E RNR + ER FN+ GGNS L+SILR + + G+QY+IG N GR R + + +++ HQA+ +++ KGR LLD+EGLSCLLILLF++D+KINT RLHRILRNLCYH PTRDWV+ L+SILE++NE K + +AS+ SF+ P R T+TP ASS +
Sbjct: 76 GAIGSSASETGAPST-----EQDYATILGMDISELPEGVDPSFLAALPEDMRQEVIDEQRRLQSMRQRAAA-HVETTGVQEVNPEFLAALPPNIQEEVLHQQRMEQQRQAAANQNPEAPVDPGEFLQTLPTSLRQSVLADMEESQMSALPADLAAEAQTLRREFEIRNRAMIQERLFNHVNQGGNSALNSILRNTFTGRGSQYVIGGFGNSRGRTRDRDGWTSHIDPGVSSL-----HQAYHTPNMKIKGRPLLDHEGLSCLLILLFIEDAKINTNRLHRILRNLCYHVPTRDWVIMSLLSILEKSNEGKSLDHSHNPAVASV--SFETPPAKIRKGATKTSGTLVETSTPTSVASSSK 421
BLAST of EMLSAG00000004991 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold186_size273091-processed-gene-0.4 (protein:Tk00902 transcript:snap_masked-scaffold186_size273091-processed-gene-0.4-mRNA-1 annotation:"e3 ubiquitin-protein ligase nedd-4") HSP 1 Score: 296.204 bits (757), Expect = 1.151e-80 Identity = 150/381 (39.37%), Postives = 222/381 (58.27%), Query Frame = 0
Query: 2576 LDFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESF---RELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYE-SSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYH-KYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHEC 2951
D + K++Y R ++ + + VRR+++F++SF R + + P K R ++ FEGE G D GGL REW+ ++S+E+FNP Y LF+ S D T I S +P+HL+YF+F+G+V A+Y LL+ +F R FYK +L + + DMES D +Y L ++ +H L +L+F + GVT DLIP G ++TVTE KLDYI V + +++Q+ AF GF IP + +F+E ELELLL G+ IDI D +T Y Y N + WFWR++ SF +A+ +QFVTG+S+VP+ GF L+G N + F I + S D P +HTCFN+LDLP Y +Y +LR+ L+ A+
Sbjct: 1211 FDSNYKKKYERFSKRLGVPKMLPPKVTIPVRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEPL--ELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEAFCI-KGFGSPDSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNA 1588 HSP 2 Score: 287.73 bits (735), Expect = 4.349e-78 Identity = 147/364 (40.38%), Postives = 220/364 (60.44%), Query Frame = 0
Query: 2577 DFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEX---KNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYE-SSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYH-KYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYE 2935
D+ K Y +++L++ + + VRR+ + E+SFR L + + K + +I F+ E G D GGL REW+ ++S+E+FNP Y LF+ S D T I S N +H+ YFKF+GR+ A+Y KLL+ +F R FYK +LGK + DMES D +Y L ++ + ++L +LTF + ++FG T R+L PNG I VT E+K DYI+ V Q + + +Q++ F GF ++P L+ IF+E ELELL+ G+ +ID+ D K+NT Y Y N + I WFWR + SF+ +++ +QFVTG+S+VP+ GF L G NG Q + I + + + P AHTCFN+LDLP YE
Sbjct: 461 DYKRKYDYLKSQLKKPSNVPNK--FEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAEL--ELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQLYTIEKWG-NPNSFPRAHTCFNRLDLPPYE 819
BLAST of EMLSAG00000004991 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold56_size446035-snap-gene-3.36 (protein:Tk11506 transcript:maker-scaffold56_size446035-snap-gene-3.36-mRNA-1 annotation:"e3 ubiquitin-protein ligase smurf1 isoform x1") HSP 1 Score: 286.574 bits (732), Expect = 6.240e-80 Identity = 142/384 (36.98%), Postives = 226/384 (58.85%), Query Frame = 0
Query: 2577 DFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGV---QKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHE-CSEVME 2956
D K + R EL+ ++ + + V R+ VFEES+R++ + P++ K R I F GE+G D GG+ REW ++S E+ NP Y LF+ S D T I S NP+HL YF FVGR+I A++ ++ FT FYK +L KP+ D+E D + L +++ + ++ + D FS E + FG+ + ++L G+++ VTE+ K +Y++L + I +Q A +GF +IP++L+ F+E+ELEL++ G+ IDI+D K++T ++ + WFW + + + +A+ +QFVTGSS+VPLQGF L+G G + F +H + S + LP AHTCFN+LDLPAYE+ K+ L +A+ E C +E
Sbjct: 600 DLVAKIKLLRQELQ--SQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGI-LDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980
BLAST of EMLSAG00000004991 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold125_size330479-snap-gene-2.15 (protein:Tk06892 transcript:maker-scaffold125_size330479-snap-gene-2.15-mRNA-1 annotation:"e3 ubiquitin") HSP 1 Score: 287.73 bits (735), Expect = 5.627e-78 Identity = 149/381 (39.11%), Postives = 231/381 (60.63%), Query Frame = 0
Query: 2577 DFDIKRRYFRTELERMDERLRREDLAVHVRRENVFEESFRELXRRSPEE-XKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSD---LGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQII-WFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHECS 2952
DFD K R F +LE L +RRE + E++F+++ S ++ K+R +I F+ EEG D GG RE++ +ISRE+FNP Y LF+ S D T + S +HLD+F+F GR++A A+ + LL+ +FTR FYK +L PV D+ES D F++ + +L ++++D LG +TFS + FG RDL P G+++ VTE+ K DYI V + ++ + Q ++GFY ++ RL+++F+ +ELEL+++G ID+ D + NTEY ++ +I WFW A+ FD + + +QFVTG+S VP +GF+ L G NG ++F I + + + LP AHTCFN+LDLP Y + ++L LL A+ E S
Sbjct: 1322 DFDAKLRSFYRKLESKGYGQGPGKLKFPMRREFLLEDAFKQVMCSSKKDLAKSRLFISFDKEEGLDYGGPSREFFFLISREVFNPYYGLFEYSANDTYTVQLSPLSSFVDHHLDWFRFCGRLLALALVNKYLLDAFFTRPFYKALLKLPVSLDDLESLDGEFHQSMVWLQENEITDPEALG--MTFSVNEEVFGKVIERDLKPGGKHMQVTEKNKKDYIERVVKWRLERGVAAQTDILVKGFYEVVDPRLLAVFDARELELVIAGTAEIDVSDWRRNTEYRSGYYDAHPVIAWFWMAIERFDNERRLRLLQFVTGTSSVPYEGFSALRGSNGPRRFCIEKWGKPSS-LPRAHTCFNRLDLPPYISPEQLLEKLLLAVEETS 1699
BLAST of EMLSAG00000004991 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold143_size313727-snap-gene-2.18 (protein:Tk09419 transcript:maker-scaffold143_size313727-snap-gene-2.18-mRNA-1 annotation:"alpha-tubulin n-acetyltransferase isoform x2") HSP 1 Score: 175.637 bits (444), Expect = 1.248e-49 Identity = 101/216 (46.76%), Postives = 135/216 (62.50%), Query Frame = 0
Query: 2955 MEFE-HLEVAFPWEITRIRSDLVPRPLESQDEHKGGATPSRKPIPHWSGVHRIVSTVLDEMGKASALAQGLNHVVTSSEKLR-HSEEQVVYILVLHSR---VAGLLKVGRKNLFLLDKSGKQNEKFPLCILDFYVAESLQRSGLGNKLFQAMLKDQGDIHPRFLAIDRPSPKLISFFEKHYQLIQNIPQVNHYHIFQGFFTDXPQXEEGNAQAKRA 3165
MEF L FP +I R+ D++P L + P + S V + +S ++D MG ASA AQ L +TS K+R HS E +VY+LV + V GLLKVG+KNLFL+D+ G QNE + +CILDFYV ES QRSG G +LF+ ML+DQG + P+F+AIDRPSPKL+ F K++ L IPQVN+Y I+ GFF + +E + KRA
Sbjct: 1 MEFAFDLNPLFPTDIVRLGPDMLPTNL----------VRAAVPFKNLSLVQQRMSFIIDCMGNASAQAQDLKQAITSGSKVRAHSGEHIVYLLVDKTSQGCVIGLLKVGKKNLFLMDRQGSQNEVYSMCILDFYVHESRQRSGCGRQLFEHMLQDQG-LDPQFMAIDRPSPKLLGFLAKYHNLSNPIPQVNNYVIYDGFFNN--NNKECSPGPKRA 203
BLAST of EMLSAG00000004991 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold22_size673200-snap-gene-2.20 (protein:Tk12673 transcript:maker-scaffold22_size673200-snap-gene-2.20-mRNA-1 annotation:"e3 ubiquitin-protein ligase hectd2") HSP 1 Score: 175.252 bits (443), Expect = 1.614e-44 Identity = 109/365 (29.86%), Postives = 185/365 (50.68%), Query Frame = 0
Query: 2601 LAVHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPV---------------KYTDMESEDYAFYKGLEFLVNHKVSDLGYDL--TFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAI 2948
L + VRR+++ E+S +E+ + + K I F GE G D GGL +EW+ ++ + IF P Y +F P + Y S + +L + +G ++ A+Y++ +L+ +F Y+ +L PV +D+ + L ++ ++ D+ DL TFS ++E+G DL PNG +ITVT E + ++++L + + IR++ +AF GF+++ + + +E+E L+ G T D+ +LK T Y Y+ + II FW L + + + KF++F TGS +VP+ G G +I R LP AHTCFNQ+ LP Y+ D+L+ L AI
Sbjct: 453 LNISVRRDHLVEDSLKEISEKQKDLKKKL-KISFTGEPGLDMGGLTKEWFQLLIKSIFEPEYGMFVYYPHSRC----YWFSLSKEGNLREYNLIGVLMGLAVYNSIILDLHFPPMCYRKLLTPPVVPANDGAPVGIVPSLTVSDLRDIMPDVTQSLANVLEYE-GDIAEDLMLTFSVSVEEYGKVFTTDLKPNGDDITVTNENRKEFVQLYLEWLLNSTIRERFNAFYLGFHSVCASNALIMLRPEEVEQLVCGSQTFDVTELKKVTFYDGYKVDDPTIICFWEVLEDYSEDLQKKFLRFTTGSDRVPVGG-------TGDMTIKITRMKDRNHHLPIAHTCFNQIGLPDYKDKDRLKRKLTIAI 804
BLAST of EMLSAG00000004991 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold363_size195477-snap-gene-0.52 (protein:Tk06661 transcript:maker-scaffold363_size195477-snap-gene-0.52-mRNA-1 annotation:"ubiquitin-protein ligase e3c") HSP 1 Score: 173.326 bits (438), Expect = 2.485e-43 Identity = 114/360 (31.67%), Postives = 189/360 (52.50%), Query Frame = 0
Query: 2601 LAVHVRRENVFEESFRELXRRSPEEXKNRFYI-----VFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDME---SEDYAFYKGLEFLVNHK--VSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLP-TIDIDDLKSNTEY-HKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAI 2948
+ V +RR ++E++F +L ++ + + + V E G D GGL RE+ + F+P F+ + D+ Y +Q P+ + ++ F+GR++ KA+Y+N L+E F +LG+ D++ S D YK L +L ++ V DLG D T +E E G T + +L+P G +I VT E +++YI +V K+ IR Q +AF +G ++I + +F+ +EL+ L+SG I++DDL+ T+Y + + + I FW+ +R + K ++FVT S+ PL GF LE F I ++RLP+A TC N L LP ++ L+T LL AI
Sbjct: 707 IRVLIRRNYIYEDAFEKLSPKNEPNLQLKMQVQLMNAVGLDEAGVDGGGLFREFMTEFLKTAFDPNRGFFRLT-RDQQLYPNPTVNQIEPDFIPHYFFIGRMLGKALYENHLVELPLASFFLSKLLGQHSVNVDIDHLSSLDPELYKNLLYLKTYEGDVVDLGLDFTMVSE--EVGTTRVHELLPGGSDIPVTNENRIEYIHIVADHKLNRQIRAQCNAFRQGLSDVINLDWLRMFSYRELQTLISGAEHEINVDDLEQCTKYGNGFDEDHPTIKTFWKVVRELSEDQKRSLLKFVTSCSRPPLLGFKELEP-----PFAIQNVGDESERLPTASTCMNLLKLPEFKDERTLKTKLLYAI 1058
BLAST of EMLSAG00000004991 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold347_size200506-processed-gene-0.2 (protein:Tk02315 transcript:snap_masked-scaffold347_size200506-processed-gene-0.2-mRNA-1 annotation:"ubiquitin-protein ligase e3b isoform x1") HSP 1 Score: 121.324 bits (303), Expect = 2.448e-27 Identity = 89/266 (33.46%), Postives = 130/266 (48.87%), Query Frame = 0
Query: 2712 FTRSFYKHILGKP----VKYTD-MESEDYAFYKGLEFLVNHKVSDLG-YDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPT-IDIDDLKSNTEYH-KYQRNSLQIIWFWRAL-RSFDQTDKAKFMQFVTGSSKVPLQGFAYLE------------------GMNGVQK--FQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAI 2948
F K +LGK Y D + S D YK L + + H D+ DLTFS + G +L+P GR+I VT K++YI V +M I+ QL+AF +GF +I + +F+ E++ L+SG +D+ DL+ +T Y+ + + IIW W L + F+ +++ F++FVT SK PL GF YLE + V + I R D +RLP+A TCFN L LP Y L+ L AI
Sbjct: 755 FLEETIKRVLGKDHTSMYSYIDELPSLDADLYKSLTY-IKHAGEDVSNLDLTFSFDQDMMGKVVNHELLPGGRSICVTNANKINYIHKVAHFRMHFQIKDQLAAFKKGFSFVIKPDWLHLFSTPEVQRLISGDSAPLDLKDLRKHTHYYGGFHDSHRVIIWLWDILEKDFNAQERSAFLRFVTSCSKSPLLGFEYLEPPFSIRCVEVGDDEDDGDTLGSVVRGFLAIPRRD-PVNRLPTASTCFNLLKLPNYHKKGTLKEKLKYAI 1018
BLAST of EMLSAG00000004991 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold114_size351134-snap-gene-1.12 (protein:Tk09543 transcript:maker-scaffold114_size351134-snap-gene-1.12-mRNA-1 annotation:"e3 ubiquitin-protein ligase hyd") HSP 1 Score: 114.39 bits (285), Expect = 4.369e-25 Identity = 80/299 (26.76%), Postives = 142/299 (47.49%), Query Frame = 0
Query: 2664 LFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGL-EFLVNHKVSDL------GYDLTFSTEI--QEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQII-----WFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAI 2948
LF T PG + Y + T P ++ F+ VGR++ + N+L +F R K++LG+ +++ D+ D Y+ L + +V+ + D +LTFS ++ +E G + +L+PNGR+I V +Y+R Q +M K L G ++++P + ++ LLL+G+ I++ L S T ++ + + + W W + +K + F TGS +P A +G + I S LPSA+TC ++L +P Y + L++ LL AI
Sbjct: 1792 LFYT-PGKRGFYSPIQGRGT-PERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSA--ELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALP----ASEDGFQPMPSVTIRPAGDS--HLPSANTCISRLYVPLYSSKAILKSKLLMAI 2080 The following BLAST results are available for this feature:
BLAST of EMLSAG00000004991 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO) Total hits: 25
Pagesback to top
BLAST of EMLSAG00000004991 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA) Total hits: 25
Pagesback to top
BLAST of EMLSAG00000004991 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self) Total hits: 12
Pagesback to top
BLAST of EMLSAG00000004991 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt) Total hits: 25
Pagesback to top
BLAST of EMLSAG00000004991 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods) Total hits: 25
Pagesback to top
BLAST of EMLSAG00000004991 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017)) Total hits: 25
Pagesback to top
BLAST of EMLSAG00000004991 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins) Total hits: 14
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Cross References
External references for this gene
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at LSalAtl2s260:299952..311628+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>EMLSAG00000004991-687757 ID=EMLSAG00000004991-687757|Name=EMLSAG00000004991|organism=Lepeophtheirus salmonis|type=gene|length=11677bp|location=Sequence derived from alignment at LSalAtl2s260:299952..311628+ (Lepeophtheirus salmonis)back to top Add to Basket
|