EMLSAG00000005727, EMLSAG00000005727-688493 (gene) Lepeophtheirus salmonis

Overview
NameEMLSAG00000005727
Unique NameEMLSAG00000005727-688493
Typegene
OrganismLepeophtheirus salmonis (salmon louse)
Associated RNAi Experiments

Nothing found

Homology
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BLAST of EMLSAG00000005727 vs. C. finmarchicus
Match: gi|592835137|gb|GAXK01122407.1| (TSA: Calanus finmarchicus comp107480_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 50.8322 bits (120), Expect = 1.456e-6
Identity = 36/109 (33.03%), Postives = 47/109 (43.12%), Query Frame = 0 
Query:   82 RPRKTGANSKYPICSYEDTNFLGADINILNSLE----TDSAYECKFDCKATKNCKFWTFDEEARRG--DNNCFLIE------------------SGTRRNNCKCLRRTD 166
            RP +TG     P CSY +T+F G D+ +    E      SA  CK  C+    C FWT+ E   R     +CFL E                  SGT+ NNC C++  D
Sbjct:  783 RPIQTGDRLPLPKCSYINTDFPGDDLILEGYTEPGINAGSARACKARCRLDDACSFWTYKEGFSRDTLTLDCFLKEGTPGLPVPREAVPRLGFVSGTQDNNCVCIKSED 1109
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BLAST of EMLSAG00000005727 vs. C. finmarchicus
Match: gi|592779700|gb|GAXK01174868.1| (TSA: Calanus finmarchicus comp723223_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 46.2098 bits (108), Expect = 4.872e-5
Identity = 38/109 (34.86%), Postives = 45/109 (41.28%), Query Frame = 0 
Query:   83 PRKTGANSKYPICSYEDTNFLGADINILN-----SLETDSAYECKFDCKATKNCKFWTFDEEARRG--DNNCFLIE------------------SGTRRNNCKCLRRTD 166
            P  TG     P CSY +T+F G DI IL+      +   SA  CK  CK    C FWT+ E   R     +CFL E                  SGT  NNC C+   D
Sbjct:  591 PVLTGDRLPLPECSYVNTDFPGDDI-ILSEDGSLGINAGSARACKARCKLENACLFWTYREGFNRDTETRDCFLKEGTPGLPVPRDAVPRIGFVSGTPDNNCICIPSED 914
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BLAST of EMLSAG00000005727 vs. C. finmarchicus
Match: gi|592779699|gb|GAXK01174869.1| (TSA: Calanus finmarchicus comp723223_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 45.8246 bits (107), Expect = 5.953e-5
Identity = 38/109 (34.86%), Postives = 45/109 (41.28%), Query Frame = 0 
Query:   83 PRKTGANSKYPICSYEDTNFLGADINILN-----SLETDSAYECKFDCKATKNCKFWTFDEEARRG--DNNCFLIE------------------SGTRRNNCKCLRRTD 166
            P  TG     P CSY +T+F G DI IL+      +   SA  CK  CK    C FWT+ E   R     +CFL E                  SGT  NNC C+   D
Sbjct:  591 PVLTGDRLPLPECSYVNTDFPGDDI-ILSEDGSLGINAGSARACKARCKLENACLFWTYREGFNRDTETRDCFLKEGTPGLPVPRDAVPRIGFVSGTPDNNCICIPSED 914
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BLAST of EMLSAG00000005727 vs. C. finmarchicus
Match: gi|592920391|gb|GAXK01037984.1| (TSA: Calanus finmarchicus comp1202161_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 41.9726 bits (97), Expect = 1.044e-3
Identity = 30/98 (30.61%), Postives = 41/98 (41.84%), Query Frame = 0 
Query:   93 PICSYEDTNFLGADI----NILNSLETDSAYECKFDCKATKNCKFWTFDEEARRG--DNNCFLIE------------------SGTRRNNCKCLRRTD 166
            P CSY +T+F G D+    +    +   SA  CK  C+    C FW++ E   R     +CFL E                  SGT  NNC C++  D
Sbjct:  289 PKCSYINTDFPGDDLLFDDDTEPGINAGSARGCKARCRQEDACSFWSYREGNNRDTFTRDCFLKEGTPGLPVPRAAVPRIGFVSGTSDNNCICIKSED 582
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BLAST of EMLSAG00000005727 vs. C. finmarchicus
Match: gi|592861309|gb|GAXK01096253.1| (TSA: Calanus finmarchicus comp55543_c4_seq10 transcribed RNA sequence)

HSP 1 Score: 40.4318 bits (93), Expect = 4.155e-3
Identity = 20/61 (32.79%), Postives = 33/61 (54.10%), Query Frame = 0 
Query:   83 PRKTGANSKYPICSYEDTNFLGADINILNSLETDSAYECKFDCKATKNCKFWTFDEEARRG 143
            P  T + S Y +C+Y   +F G D   L S+  +   +C+  C+   +C+ +TFD+E  RG
Sbjct: 2577 PTPTDSPSTYSVCTYNSLDFQGGD---LFSVLANDVDDCRQQCEDHVSCQLFTFDKELERG 2750

HSP 2 Score: 30.4166 bits (67), Expect = 6.384e+0
Identity = 21/63 (33.33%), Postives = 33/63 (52.38%), Query Frame = 0 
Query:   93 PICSYED-TNFLGADINIL---NSLET--DSAYECKFDCKATKNCKFWTFDEEARRGDNNCFL 149
            P C Y D T F+G D+  +     +ET  +S+ +C  +C+    CK+WT+    +    NCFL
Sbjct: 2829 PQCLYNDQTEFVGGDLPEVFGGGGVETSMESSADCIEECEKRSGCKYWTWVMGEKV---NCFL 3008
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BLAST of EMLSAG00000005727 vs. C. finmarchicus
Match: gi|592861310|gb|GAXK01096252.1| (TSA: Calanus finmarchicus comp55543_c4_seq9 transcribed RNA sequence)

HSP 1 Score: 40.4318 bits (93), Expect = 4.161e-3
Identity = 20/61 (32.79%), Postives = 33/61 (54.10%), Query Frame = 0 
Query:   83 PRKTGANSKYPICSYEDTNFLGADINILNSLETDSAYECKFDCKATKNCKFWTFDEEARRG 143
            P  T + S Y +C+Y   +F G D   L S+  +   +C+  C+   +C+ +TFD+E  RG
Sbjct: 2628 PTPTDSPSTYSVCTYNSLDFQGGD---LFSVLANDVDDCRQQCEDHVSCQLFTFDKELERG 2801

HSP 2 Score: 30.4166 bits (67), Expect = 6.391e+0
Identity = 21/63 (33.33%), Postives = 33/63 (52.38%), Query Frame = 0 
Query:   93 PICSYED-TNFLGADINIL---NSLET--DSAYECKFDCKATKNCKFWTFDEEARRGDNNCFL 149
            P C Y D T F+G D+  +     +ET  +S+ +C  +C+    CK+WT+    +    NCFL
Sbjct: 2880 PQCLYNDQTEFVGGDLPEVFGGGGVETSMESSADCIEECEKRSGCKYWTWVMGEKV---NCFL 3059
[Close]
BLAST of EMLSAG00000005727 vs. C. finmarchicus
Match: gi|592861311|gb|GAXK01096251.1| (TSA: Calanus finmarchicus comp55543_c4_seq8 transcribed RNA sequence)

HSP 1 Score: 40.4318 bits (93), Expect = 4.163e-3
Identity = 20/61 (32.79%), Postives = 33/61 (54.10%), Query Frame = 0 
Query:   83 PRKTGANSKYPICSYEDTNFLGADINILNSLETDSAYECKFDCKATKNCKFWTFDEEARRG 143
            P  T + S Y +C+Y   +F G D   L S+  +   +C+  C+   +C+ +TFD+E  RG
Sbjct: 2643 PTPTDSPSTYSVCTYNSLDFQGGD---LFSVLANDVDDCRQQCEDHVSCQLFTFDKELERG 2816

HSP 2 Score: 30.4166 bits (67), Expect = 6.393e+0
Identity = 21/63 (33.33%), Postives = 33/63 (52.38%), Query Frame = 0 
Query:   93 PICSYED-TNFLGADINIL---NSLET--DSAYECKFDCKATKNCKFWTFDEEARRGDNNCFL 149
            P C Y D T F+G D+  +     +ET  +S+ +C  +C+    CK+WT+    +    NCFL
Sbjct: 2895 PQCLYNDQTEFVGGDLPEVFGGGGVETSMESSADCIEECEKRSGCKYWTWVMGEKV---NCFL 3074
[Close]
BLAST of EMLSAG00000005727 vs. C. finmarchicus
Match: gi|592861312|gb|GAXK01096250.1| (TSA: Calanus finmarchicus comp55543_c4_seq7 transcribed RNA sequence)

HSP 1 Score: 40.4318 bits (93), Expect = 4.169e-3
Identity = 20/61 (32.79%), Postives = 33/61 (54.10%), Query Frame = 0 
Query:   83 PRKTGANSKYPICSYEDTNFLGADINILNSLETDSAYECKFDCKATKNCKFWTFDEEARRG 143
            P  T + S Y +C+Y   +F G D   L S+  +   +C+  C+   +C+ +TFD+E  RG
Sbjct: 2694 PTPTDSPSTYSVCTYNSLDFQGGD---LFSVLANDVDDCRQQCEDHVSCQLFTFDKELERG 2867

HSP 2 Score: 30.4166 bits (67), Expect = 6.399e+0
Identity = 21/63 (33.33%), Postives = 33/63 (52.38%), Query Frame = 0 
Query:   93 PICSYED-TNFLGADINIL---NSLET--DSAYECKFDCKATKNCKFWTFDEEARRGDNNCFL 149
            P C Y D T F+G D+  +     +ET  +S+ +C  +C+    CK+WT+    +    NCFL
Sbjct: 2946 PQCLYNDQTEFVGGDLPEVFGGGGVETSMESSADCIEECEKRSGCKYWTWVMGEKV---NCFL 3125
[Close]
BLAST of EMLSAG00000005727 vs. C. finmarchicus
Match: gi|592861313|gb|GAXK01096249.1| (TSA: Calanus finmarchicus comp55543_c4_seq6 transcribed RNA sequence)

HSP 1 Score: 40.4318 bits (93), Expect = 4.213e-3
Identity = 20/61 (32.79%), Postives = 33/61 (54.10%), Query Frame = 0 
Query:   83 PRKTGANSKYPICSYEDTNFLGADINILNSLETDSAYECKFDCKATKNCKFWTFDEEARRG 143
            P  T + S Y +C+Y   +F G D   L S+  +   +C+  C+   +C+ +TFD+E  RG
Sbjct: 3128 PTPTDSPSTYSVCTYNSLDFQGGD---LFSVLANDVDDCRQQCEDHVSCQLFTFDKELERG 3301

HSP 2 Score: 30.4166 bits (67), Expect = 6.448e+0
Identity = 21/63 (33.33%), Postives = 33/63 (52.38%), Query Frame = 0 
Query:   93 PICSYED-TNFLGADINIL---NSLET--DSAYECKFDCKATKNCKFWTFDEEARRGDNNCFL 149
            P C Y D T F+G D+  +     +ET  +S+ +C  +C+    CK+WT+    +    NCFL
Sbjct: 3380 PQCLYNDQTEFVGGDLPEVFGGGGVETSMESSADCIEECEKRSGCKYWTWVMGEKV---NCFL 3559
[Close]
BLAST of EMLSAG00000005727 vs. C. finmarchicus
Match: gi|592861314|gb|GAXK01096248.1| (TSA: Calanus finmarchicus comp55543_c4_seq5 transcribed RNA sequence)

HSP 1 Score: 40.4318 bits (93), Expect = 4.215e-3
Identity = 20/61 (32.79%), Postives = 33/61 (54.10%), Query Frame = 0 
Query:   83 PRKTGANSKYPICSYEDTNFLGADINILNSLETDSAYECKFDCKATKNCKFWTFDEEARRG 143
            P  T + S Y +C+Y   +F G D   L S+  +   +C+  C+   +C+ +TFD+E  RG
Sbjct: 3154 PTPTDSPSTYSVCTYNSLDFQGGD---LFSVLANDVDDCRQQCEDHVSCQLFTFDKELERG 3327

HSP 2 Score: 30.4166 bits (67), Expect = 6.451e+0
Identity = 21/63 (33.33%), Postives = 33/63 (52.38%), Query Frame = 0 
Query:   93 PICSYED-TNFLGADINIL---NSLET--DSAYECKFDCKATKNCKFWTFDEEARRGDNNCFL 149
            P C Y D T F+G D+  +     +ET  +S+ +C  +C+    CK+WT+    +    NCFL
Sbjct: 3406 PQCLYNDQTEFVGGDLPEVFGGGGVETSMESSADCIEECEKRSGCKYWTWVMGEKV---NCFL 3585
[Close]
BLAST of EMLSAG00000005727 vs. L. salmonis peptides
Match: EMLSAP00000005727 (pep:novel supercontig:LSalAtl2s:LSalAtl2s309:715420:717244:1 gene:EMLSAG00000005727 transcript:EMLSAT00000005727 description:"maker-LSalAtl2s309-augustus-gene-7.78")

HSP 1 Score: 471.855 bits (1213), Expect = 3.378e-170
Identity = 232/232 (100.00%), Postives = 232/232 (100.00%), Query Frame = 0 
Query:    1 MNILPLSFVVFLISIKEVTCQSLEEVSSLLSSNEKLVDENVEVFKEDFVEFFYAPSRYFRYRNRYRRFYPQYYNIRNQFVKRPRKTGANSKYPICSYEDTNFLGADINILNSLETDSAYECKFDCKATKNCKFWTFDEEARRGDNNCFLIESGTRRNNCKCLRRTDFIEVENEPDTDIGSDARRRRRRRRNRPKICPRGVKESDDIDYTYDDYEEFNENLKKKSQNSTMSGA 232
            MNILPLSFVVFLISIKEVTCQSLEEVSSLLSSNEKLVDENVEVFKEDFVEFFYAPSRYFRYRNRYRRFYPQYYNIRNQFVKRPRKTGANSKYPICSYEDTNFLGADINILNSLETDSAYECKFDCKATKNCKFWTFDEEARRGDNNCFLIESGTRRNNCKCLRRTDFIEVENEPDTDIGSDARRRRRRRRNRPKICPRGVKESDDIDYTYDDYEEFNENLKKKSQNSTMSGA
Sbjct:    1 MNILPLSFVVFLISIKEVTCQSLEEVSSLLSSNEKLVDENVEVFKEDFVEFFYAPSRYFRYRNRYRRFYPQYYNIRNQFVKRPRKTGANSKYPICSYEDTNFLGADINILNSLETDSAYECKFDCKATKNCKFWTFDEEARRGDNNCFLIESGTRRNNCKCLRRTDFIEVENEPDTDIGSDARRRRRRRRNRPKICPRGVKESDDIDYTYDDYEEFNENLKKKSQNSTMSGA 232
[Close]
BLAST of EMLSAG00000005727 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold440_size170678-snap-gene-0.31 (protein:Tk09656 transcript:maker-scaffold440_size170678-snap-gene-0.31-mRNA-1 annotation:"coagulation factor xi-like")

HSP 1 Score: 55.4546 bits (132), Expect = 2.445e-9
Identity = 32/92 (34.78%), Postives = 42/92 (45.65%), Query Frame = 0 
Query:   89 NSKYPICSYEDTNFLGADINILNSLETDSAYECKFDCKATKNCKFWTFDEEARRGDNNCFL-----------------IESGTRRNNCKCLR 163
            +S  P CSY +T+F G D+     L T S Y CK  C     C+FWTF  +  R   +C+L                   SG+R N+C CLR
Sbjct:  205 DSLLPKCSYINTDFEGDDVGDGRGLTTGSPYSCKQACIDQNGCRFWTFRRDWAR---DCYLKRGEDAGRPNNVRRRAGFVSGSRSNDCSCLR 293
The following BLAST results are available for this feature:
BLAST of EMLSAG00000005727 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000005727 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA)
Total hits: 25
Match NameE-valueIdentityDescription
gi|592835137|gb|GAXK01122407.1|1.456e-633.03TSA: Calanus finmarchicus comp107480_c0_seq1 trans... [more]
gi|592779700|gb|GAXK01174868.1|4.872e-534.86TSA: Calanus finmarchicus comp723223_c0_seq1 trans... [more]
gi|592779699|gb|GAXK01174869.1|5.953e-534.86TSA: Calanus finmarchicus comp723223_c0_seq2 trans... [more]
gi|592920391|gb|GAXK01037984.1|1.044e-330.61TSA: Calanus finmarchicus comp1202161_c0_seq1 tran... [more]
gi|592861309|gb|GAXK01096253.1|4.155e-332.79TSA: Calanus finmarchicus comp55543_c4_seq10 trans... [more]
gi|592861310|gb|GAXK01096252.1|4.161e-332.79TSA: Calanus finmarchicus comp55543_c4_seq9 transc... [more]
gi|592861311|gb|GAXK01096251.1|4.163e-332.79TSA: Calanus finmarchicus comp55543_c4_seq8 transc... [more]
gi|592861312|gb|GAXK01096250.1|4.169e-332.79TSA: Calanus finmarchicus comp55543_c4_seq7 transc... [more]
gi|592861313|gb|GAXK01096249.1|4.213e-332.79TSA: Calanus finmarchicus comp55543_c4_seq6 transc... [more]
gi|592861314|gb|GAXK01096248.1|4.215e-332.79TSA: Calanus finmarchicus comp55543_c4_seq5 transc... [more]

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BLAST of EMLSAG00000005727 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self)
Total hits: 1
Match NameE-valueIdentityDescription
EMLSAP000000057273.378e-170100.00pep:novel supercontig:LSalAtl2s:LSalAtl2s309:71542... [more]
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BLAST of EMLSAG00000005727 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000005727 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000005727 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017))
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000005727 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins)
Total hits: 1
Match NameE-valueIdentityDescription
maker-scaffold440_size170678-snap-gene-0.312.445e-934.78protein:Tk09656 transcript:maker-scaffold440_size1... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
LSalAtl2s309supercontigLSalAtl2s309:715420..717244 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
ensembl2013-09-26 .965016
Blast vs. GO2014-04-02
TblastN vs C. finmarchicus TSA2014-05-09
Blastp vs. self2014-05-10
Blastp vs. SwissProt2017-02-10
Blastp vs. Selected Arthropods2017-02-20
Blastp vs. NR (2/2017)2017-02-20
Blastp vs. Tigriopus kingsejongensis proteins2018-04-18
Properties
Property NameValue
Logic nameensemblgenomes
Descriptionmaker-LSalAtl2s309-augustus-gene-7.78
Biotypeprotein_coding
EvidenceIEA
NoteHypothetical protein
Cross References
External references for this gene
DatabaseAccession
Ensembl Metazoa (gene)EMLSAG00000005727 (primary cross-reference)
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
EMLSAT00000005727EMLSAT00000005727-701574Lepeophtheirus salmonismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at LSalAtl2s309:715420..717244+

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>EMLSAG00000005727-688493 ID=EMLSAG00000005727-688493|Name=EMLSAG00000005727|organism=Lepeophtheirus salmonis|type=gene|length=1825bp|location=Sequence derived from alignment at LSalAtl2s309:715420..717244+ (Lepeophtheirus salmonis)
TCGATACACTCTTTTCAGTAAGCGAAGTATGCCTACATAATTTATTATCG
TAATATGGTTTGATGTACATTTTTTTACTCATTACCACTGTTGACTTCGA
GACGTTATGAACATTTTACCACTTAGTTTCGTTGTTTTTCTAATAAGTAT
CAAAGAGGTCACATGTCAATCCTTGGAAGAAGTGAGTTCTCTATTGTCGA
GCAATGAAAAGTTAGTGGATGAGAATGTAGAAGTCTTTAAGGAGGATTTC
GTTGTGAGTACTGTTAATCAGTCCTTTTTTTTCTCTTAAAAAATCAATTG
CATGCAATCATTATTACCTAGTTACTAGTTTATAACTAATTTATTCATCG
TCTGAAAAAAATATGTTAATGTATAGCTACACTTTTAATCAAATATGATA
TTAATTCAGTAGACTTAGTTAATTAATATCTACTATTAAATCTATCTCAC
ATTTGTCATTGATGAACAACTTTCAGAAAAAAGAAAATGAGGAGTTTTTT
TATGCTCCGTCAAGGTACTTTCGATATAGAAATCGTTATAGACGATTTTA
TCCCCAATATTATAATATAAGAAATCAATTTGTGAAAAGACCTCGGAAAA
CCGGAGCCAACTCTAAATACCCCATTTGCTCTTATGAAGATACTAATTTC
CTTGGAGCAGATATAAATATATGTAAGTTCAATGGAAATCATCATTGGTT
CATTTAATCAATGGACTTTTATTTTTTCTAGTAAACTCCTTAGAGACTGA
CTCGGCGTACGAATGTAAATTTGATTGCAAAGCGACTAAAAATTGCAAAT
TTTGGACTTTTGATGAAGAGGCCCGTCGAGGGGATAACAATTGCTTCCTG
ATTGAATCAGGTAAGTCATAATTTGCAGAGGTGGGGATCTAGACTCGAGT
TCATAATTTGAAGACTCGATTGTATATCCAATCCATGGATCGCAACTTGA
ATAGGACTCGAAAGCTTAGACTTGATACTTCAATTGATTTTTTTTTTGCA
ATCGTCACAATCATTTCTAAGAGGTATACAAAAAGTTTTGGCCGCATATT
TGTGTCTAGGATTTTTTTTAAACTTTTTAATTACCTTTTTCCTGGACATT
TCTTTTTTTGATGACATTGCGGAGCCCCAGTTGGGAATCACTGCTTTATG
GTCTATATATCGGTATTGTTAATACTTAAAAAAAATTCCCTCAGGAAAAT
TTTCACTGTCATTTTTTATTTTCCATGGGAAATAAAGTAATCTTTAAGGG
GAAAGATTTAATTGGAGTAAGATTATTTTATACATGAAATATTCGAGAAC
TAGGACTTGAGACTCGACTTGCAGAATTTTCCCACCTCGAAAAAAAATAA
TTTGTGTCCTTCTAGATGTTATCCTTTTGAGGGGGGTAGGTGAAAGTCAT
GTTAAACATCTTTAAAACTGAAACWTTTTTWTTTWTTTGMYGGGYGGGGG
GYYGAGGCTAGATCCCCTGAATAGATTAATTTTAATATGCTAAGTTTATG
ATCTGCTGCTTTTCTTTTATGCGTAATTTAGGTAAAGGCCGAACCCCAAA
NCAATGTGTTCCTAGATAGGGATTACATTTCAGGGACAAGACGAAACAAT
TGTAAGTGCTTGAGGAGAACAGATTTCATTGAAGTGGAGAATGAGCCTGA
TACTGATATTGGATCTGATGCGAGGCGTAGACGACGTAGACGTCGAAACA
GACCAAAAATTTGTCCTCGAGGTGTCAAAGAATCAGATGACATTGACTAT
ACTTATGACGACTATGAAGAATTTAATGAAAATTTGAAGAAAAAATCTCA
AAATTCAACCATGAGTGGTGCTTAG
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