LsHSCARB - a putative intestinal heme receptor

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General

End Date: 

Monday, May 29, 2017 - 10:10

Experiment Description: 

Knock-down of putative heme receptor

Experiment Batch ID: 

EMLSAT00000005382

Start Date: 

Tuesday, March 21, 2017 - 10:10

Experiment Contact: 

Erna Irene Heggland

Publication: 

A scavenger receptor B (CD36)-like protein is a potential mediator of intestinal heme absorption in the hematophagous ectoparasite Lepeophtheirus salmonis
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

30

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Primary target ID: 

EMLSAG00000005382, EMLSAG00000005382-688148 (gene) Lepeophtheirus salmonis
Fragment description

Fragment Label ID: 

F289

Fragment concentration: 

600.00ng/ul

Fragment ID: 

EMLSAG00000005382, EMLSAG00000005382-688148 (gene) Lepeophtheirus salmonis

Fragment length: 

731bp

Sense sequence: 

agcggataaactcgatggcttgccaaaaacagtaaaagatatttgttttgaaattataaataatggtaatgaagctctaggtattaaggccgaagatttatttattactgaagaagttgacaaaatcctatacacgggcttcgactctaagtctgctgctatctttgataaattggatacatttcttatgctcttgttagaagtcatacaagaatccttggagttggatataccaattaaagcaaaagactttgaaaacataatcaaaatcattagtcctgcacagctttcagaagggacttttgcttttttcaaaggtaaaaatgcaaccaaattgcagaattactataccattgaaaacggacggttcgataaagaaagttttatgaatattgttgagttcaatggtaagaataaattacctgaggcttggtggcctaatgtagccacaagtataacgggtcaactttcaagtgagggtggctcatgccatagaatctatggaacggatgggactcaatttccaccgtttttatttaacaagaaaaaatttcctttgtggatgtttgtgggagagctttgtagaacaatatatgtggagtttgagtccgaagtggaagtggaaggagggatcacagcctatcgttatggagtagggaagagagttttctcaatgagtaaccctgaaaacttttgttattgtcaagaatttttctcatgcgccaagcaaa
Validation

Efficacy of Knock Down: 

F>95.00%
Phenotype

Phenotype CV: 

Phenotype: 

Shorter egg-strings, 60% less heme, lower hatching rate

Number of Individuals (End): 

15

Gallery Image: 

Image file: 

Image icon Control_lice_6a.jpg
Image icon Control_lice_6b.jpg
Image icon Control_lice_6c.jpg
Image icon RNAi_lus_7a.jpg
Image icon RNAi_lus_7b.jpg
Image icon RNAi_lus_7c.jpg
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Characterization of a putative intestinal heme scavenger.

Experiment terminated at control lice's 5 pair of egg-strings. We only start to see a phenotypic change at the 2nd and 3rd pair of egg-strings (not shown here). Treated lice had shorter egg-strings with a lower hatching rate, and the adult females had about 60% less heme (measured with fluorescence). Animals were disected into two poritons. We measured down-regulation by RNAi in the intestinal tissue and heme levels were measured in the remaining body tissues. See Materials&Methods for details.