BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000007725 (supercontig:LSalAtl2s:LSalAtl2s44:25674:28922:-1 gene:EMLSAG00000007725 transcript:EMLSAT00000007725 description:"maker-LSalAtl2s44-augustus-gene-0.13")

HSP 1 Score: 577.785 bits (1488), Expect = 0.000e+0
Identity = 353/1017 (34.71%), Postives = 538/1017 (52.90%), Query Frame = 0

Query:   78 QVNC-RGGVEGSHEW-GMYVARHPILVIVVSLLITSLSGLGFLRFSSESRAEKLWIPSDSPYLENKAWLDKNYPQTRRNHLALFVAEKNILTPEGIMEMLIMHQKIHAISVN-NKTYQDLCFKLPIANI-----FLTKRRRRRQAEDNSVSTLNPLNSTSAIVPALASSSNDSSGAGTNSSSPSNATSSEAEESSNREEKFLFNFGDDPSDDPQDTLSPQIYCDLVSTLDEKCLENSLLEIW------RYSESRVKRLTQEEILYAVNQVAESPWFGYRTDFSVYLGAIERNS-SGHIVAAKSALYSWTAAFD-PDGELYEEGGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRNWIPSAWTQKE---------YGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFP-TNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIIL---EKELVCNQPASRITATNSDFSYVKF------SGPEEHVPGRRLVENLIQGKKFSS--KAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVE-EGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTSSHDEDAASTAGGSSINTNSTNEDNY-KPSPLSAGLMNE 1055
            Q NC   G+E   +W G ++ +HP+ VI +       S  GF  +  E R  KLW    +P   N  +L + +P   R    + V   N+L P  I  M  M +KI  I  N   T +  C K+P+        F+  +RR+R+  D+  ++    N  S           D+SG   +  S     S EA                           P+ YC++V +++E C E+S+LE+W        +E R++ L+  +IL  +N    S  F  + +F+  LG I R+  +  IV AK+    W    +  +       G GV   + DK +++FEKEM+++LL+ +   E   G+KI  +V+RSF DI    I  D   +  GY ++FFY +L LGK + V  R ++A  GIISV  G+  + G+ SA+G  ++P+H ILPFL LGIGIDDMF+I+QCW  L+P E+  +  +K G TM+HAGVAITVTSLTD+ AFG+G  + LP L++FC+  A+ IAAIY  Q ++FVA +  D++RI   R+G  PCI  +    +     E           K+F   +A  LK    K +++ IT+G    G YG YLI Q+FDP+  +P  +YL  W       F    G+  N+Y   + D+  DL + E++   + A+     I+ S +SW   +  +++T          + EG     ++ FL S +G +YK   +      +  C + ++    +N    Y  F      S  +  +     +++++     +S  + F  S+ Y+ WETDE+I +EL++N+  A V VFI+T ++++N     +VL  + LT++D+ G +HFWGLTIDT+SC  ++++IGLCVDYS H+AH F++E EG R++R + +L ++GPA++NGGI+TFLA  LL  S SH FITFFKVF L V FGLFHGLV+LPVILSL+GP  S+  D    +    I+ N  +  N   PS L   +  E
Sbjct:   48 QTNCIVRGLESYFQWHGRFIGKHPVFVICLCFAFLLASFAGFPYYRKEERRIKLWSSDKTPSRLNHEFLHEYFPSDFR-FANVIVEADNVLDPAAIXAMFQMRKKISEIKTNYGDTLRTKCAKMPVVKTPNFLSFIFGKRRKRRVADDFFTSDYESNFFSDSFEHEMEEEEDNSGFDKDDISYPERLSVEA--------------------------YPEPYCNIVDSMEETCFESSVLELWGDNGEYESTEKRLESLSLIDILDTINNKNRSGIFLIKKNFTHMLGGITRDPITKEIVGAKATRMFWFLKMNIAEARKSPVEGQGV---IVDKKTMEFEKEMLDILLNDTGLPE---GLKISPHVSRSFFDIAGPTILGDVKILGIGYSIVFFYVLLMLGKFDWVGXRXYLAIAGIISVVFGIVFSIGICSAMGFFWSPMHSILPFLLLGIGIDDMFIIIQCWNTLNPYEKASSSVDKFGYTMRHAGVAITVTSLTDIVAFGIGGTSXLPALRSFCIFSAVGIAAIYYFQVTFFVAWMVFDEKRISSGRNGCCPCIEVKKVKKNTEEIMEDMEVESLSIMSKIFRF-YASHLKFKPVKFLIILITMGFTSLGAYGNYLIEQKFDPIWFIPPHTYLSDWFEANTKWFSDKGGFFVNVYVYEV-DLSVDLMELERI---IRAIESQSDIIDSVNSWTTNYIKYVDTHG--------LAEGTLYERITQFLHSPMGLQYKRNFLTVNGTADFDCGKSSTGFFNSNESIWYFNFNYKVLESDSKIKISAMNRIKDILNSANLTSSGRIFPLSQGYSMWETDEIIGYELYKNIIFAFVAVFIMTWIVISNFVASFLVLFAVMLTVLDVSGIMHFWGLTIDTVSCNFLIISIGLCVDYSTHVAHRFLIEAEGSRDQRIVKALTNIGPAVMNGGISTFLAFCLLCGSSSHVFITFFKVFFLVVTFGLFHGLVFLPVILSLIGPI-SYGSDTVDRSQSVIISDNKQDSINLVSPSKLKDSVDKE 1017          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000010036 (supercontig:LSalAtl2s:LSalAtl2s660:189355:234598:1 gene:EMLSAG00000010036 transcript:EMLSAT00000010036 description:"maker-LSalAtl2s660-augustus-gene-1.6")

HSP 1 Score: 344.354 bits (882), Expect = 1.346e-107
Identity = 179/482 (37.14%), Postives = 290/482 (60.17%), Query Frame = 0

Query:  120 FSSESRAEKLWIPSDSPYLENKAWLDKNYPQTRRNHL-ALFVAEKNILTPEGIMEMLIMHQKIHAISV-NNKTYQDLCFKLPIANIFLTKRRRRRQAEDNSVSTLNPLN-STSAIVPALASSSNDSSGAGTNSSSPSNATSSEAEESSNREEKFLFNFGDDPSDDPQDTLSPQIYCDLVSTLDEKCLENSLLEIWRYSESRVKRLTQEEILYAVNQVAESPWFGYRTDFSVYLGAIERNSSGHIVAAKSALYSWTAAFDPDGELYEEGGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNL--------SPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYI 590
            +S E + EKLWIP +S ++ NK W+++N+P   R  + +L     NILTPE ++ +L +H+K+  IS  +N  ++DLCFK+P+AN+FL + +     ++ +   ++ L  S   +VP    +  D   A     + S        ++ +R EK L              LSP++YCD V TL+++C E SLLEIW++   R+KRLT++ I+ A+N++  SP +GY+T++  Y+G+I RN +GH+V+A +    W    + +        +G+  +  +K SL++E + +++ L+ S E+    G+      ARSFNDIT ++IFFD +++  G+F+M  YT++ LG+LN VE R +    G+ S+ +G+ ++ G+ SA+G+ Y+P+  +LPFL LGIGIDDMF+IVQCWYN+        S ++  +TL E +  TMK AGV IT+TSLTD+FAFG+G +T         V+  I +A +YI
Sbjct:   21 YSFEYKMEKLWIPQNSEFISNKKWIEENFPTNYRVQIISLLSLSGNILTPEALLLLLDIHEKVEQISAGHNTRWKDLCFKVPVANVFLERNKVLTNDKNTTFDRVDDLFISDDNVVPQNEKTEIDYDDAFLEYENES--------KNGSRYEKLL------------KILSPKVYCDFVETLNDRCFERSLLEIWKFDRRRIKRLTKDRIVQAINRIQNSPVYGYKTNYDNYMGSIIRNETGHVVSASTLNSVWFTQVNLENV---SEVIGLTDEFVNKESLEWELKFVDIFLNASTENISAFGIS-----ARSFNDITKKSIFFDGIRLTGGFFIMCIYTVIMLGRLNCVEVRFFATIAGLFSILLGMVVSLGIISAMGLKYSPVFAMLPFLYLGIGIDDMFIIVQCWYNILEQNKKECSTDQSIKTLEEMMAETMKDAGVTITITSLTDIFAFGIGGIT---------VSQVIYVAFMYI 465          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000003602 (supercontig:LSalAtl2s:LSalAtl2s197:152115:159124:1 gene:EMLSAG00000003602 transcript:EMLSAT00000003602 description:"maker-LSalAtl2s197-snap-gene-1.29")

HSP 1 Score: 350.903 bits (899), Expect = 1.338e-105
Identity = 243/756 (32.14%), Postives = 402/756 (53.17%), Query Frame = 0

Query:  288 PQIYCDLVSTLDEKCLENSLLEIWRYSESR-----VKRLTQEEILYAVNQ-VAESPWFGYRTDFSVYLGAIERNSSGHIVAAKSALYSWTAAFDPDGELYEEGGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSP---EEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPC-IRYRNWIPSAWTQKEYGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPT-NGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTMVREGNFEMFLSDFLF-SKVGSRYKPKIILEKELVCNQPAS--RITATNSDFSYVKFSGPEEHVPGRRLVENL---IQGKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFI-VEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTSSHDEDAA 1025
            P   C  ++  +  C   S+ EIW   ES      + +LT E+IL A++Q +A S  F  R  F   +G+   N +G +  AK+ L S++   +      ++ G+ +           +EK + E + + S   +E S + +    +  F       +  D      GY ++F Y +  LG++N +EH+ ++A  GI  VGMG   + G+   L +  TP++ ILPF+ LGIGIDDMFVIVQ   N+     EE +  +   IG  MK AG AITVTSLTD+ AFG+G  + LP L+ FC+  +  I  +Y    ++F+    +D RR+  HRD   PC I+  +W+P  +T+K + +   +    ++   F + + + +T+ V G G+YG  LI  +FDP   +  +S L  +    E HF    G     Y   + +  N L + EK+  E+    K+ + ++  +S     K F +T  + T+++           L++FL+ S+V   +  K    ++ VC       +++A    F ++K +  ++ +   + +  +   I+G++ +   F  +  Y+   T ++I  EL RN+ LA++ VFI  L LLA     + VL  I L+++D+ G+++FWG++IDT S + + +++GL VDYSAHIAH +I   +  R E+  +SL  +GPA+L+GGI+TFLA +LL  S S  F TFFK+F L V+FGL+HGL++LPV+LS +GP     +D+ 
Sbjct:  148 PSEICRALANAEWLCSAKSIFEIWTSDESSKEASIIAQLTDEDILDALSQDLAISKLF--RRPFRPIIGSKMFNDNGTLKGAKALLLSYSIKTNSS----DKNGIPI-----------WEKSVKEHVFNYS-FGDESSNLSVTLFSSSIFRAEFQNGMENDVQLFIFGYLIVFVYIIFNLGEMNTLEHKFYLAVVGIACVGMGYASSVGIFQLLSVYATPMNSILPFMLLGIGIDDMFVIVQASKNVKQNDDEEGENKMDSYIGNIMKSAGXAITVTSLTDILAFGIGGFSQLPALRNFCLYSSAGIFFVYFYIMTFFLGWFVIDARRVESHRDACLPCIIKKSSWVP-CYTRKSFAEXLFSCSGVLIDSLFFRGLAIIVTIVVSGFGVYGLTLIEPKFDPKSYINPESKLLSYFKASEQHFQKLGGIPGQFY---ILNPNNSLVEIEKVYEEL----KNSEFIEE-NSLRTFHKAFSKTINSTTNFQYSS--------LTNFLYTSRVPFDWAVKFKASQKPVCGDTTEDVKLSAFVLYFQHIKKTRVQDQIQSMKKIYQIGEKIEGER-NGLIFPHATQYSNLITLDIILSELTRNILLALMTVFIAALFLLAXALTSIYVLLSILLSIIDVTGYMYFWGVSIDTASAVLLTVSLGLAVDYSAHIAHGYIHSSKSSRGEKVKDSLTKVGPAVLSGGISTFLAFILLANSSSIVFTTFFKIFFLVVLFGLYHGLIFLPVLLSFIGPVYRQVQDST 867          

HSP 2 Score: 59.3066 bits (142), Expect = 5.764e-9
Identity = 37/113 (32.74%), Postives = 62/113 (54.87%), Query Frame = 0

Query:   89 HEWGMYVARHPILVIVVSLLITSLSGLGFLRFSSESRAEKLWIPSDSPYLEN-KAWLDKNYPQTRRNHLALFVAEK--NILTPEGIMEMLIMHQKIHAISV-NNKTYQDLCFK 197
            +++GM VA++  + I +SL I   S LGF+ + +ES   K+W+P DS Y++N K   D N        +   + E   NIL  + +  +  +H  I  +   +N+T++DLCFK
Sbjct:   25 YQYGMIVAKYSYVFIGLSLGIFIASTLGFIDYVTESDPTKIWLPQDSYYVQNTKKIKDLNSDAVNAATVIQVIVESEVNILRAKELNAVYKLHSSIINLKAPSNETFKDLCFK 137          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000007475 (supercontig:LSalAtl2s:LSalAtl2s429:50360:104533:1 gene:EMLSAG00000007475 transcript:EMLSAT00000007475 description:"maker-LSalAtl2s429-augustus-gene-1.9")

HSP 1 Score: 319.701 bits (818), Expect = 1.221e-99
Identity = 172/433 (39.72%), Postives = 253/433 (58.43%), Query Frame = 0

Query:  570 ILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPC-IRYRNWIPSAWTQKEYGKMFMAGFAKILKKGFTKIVV--------LGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTMVREGN---FEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFK 990
            ILPGL+ F +TCAI IAA+++ Q SWF A   LDQ+RI +++ GF PC I+Y +      +++E GK F   F   L + F++ ++        + I+L +F  G++G + IRQEFD    +P+ SY  QW  ++E H  + GW +NIY G + D   D+ +   +   +  L      + + + WW  FK +I   +NV+ WR+ + + +   F + LSDFL+S +G++YK     +  L CNQPA  I                                           +Y+ WET+E+I FELWRN+ +A + +F+I  + LAN+ +  +VL  + +T+VD+VGFLHFWG+TIDTI+C+NIVL+IGLCVDYS HIAH F+   GD  ERA +S+ S+G AI+NGG TTF+AL+LL FSESH FI FF+
Sbjct:    1 ILPGLRHFSITCAIGIAAVFLCQISWFSAWFYLDQKRILNNKSGFFPCCIQYEDT-----SERENGKCFEFSFGNKLIRAFSRTIMHRTFQVIVIIISLILFSFGVWGAFSIRQEFDIKKFMPSSSYFVQWFEIKEKHSVSKGWQSNIYLGRI-DPPLDIPKLNSVDEFLQDLLDSKAYIITKEVWWTDFKLYILRDKNVSHWRSTLFKNDSFLFRVHLSDFLYSSIGAQYKVNFRFDTPLRCNQPAPNI------------------------------------------NIYSIWETEEIIVFELWRNMGIACIVIFVILSLFLANLLVSFLVLCNVIITVVDIVGFLHFWGITIDTIACVNIVLSIGLCVDYSIHIAHFFLCASGDAAERAASSVESIGIAIINGGTTTFMALILLIFSESHIFIYFFQ 385          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000005221 (supercontig:LSalAtl2s:LSalAtl2s276:209809:212602:-1 gene:EMLSAG00000005221 transcript:EMLSAT00000005221 description:"maker-LSalAtl2s276-augustus-gene-2.14")

HSP 1 Score: 312.383 bits (799), Expect = 2.009e-93
Identity = 192/598 (32.11%), Postives = 337/598 (56.35%), Query Frame = 0

Query:  446 MAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRNWIP--SAWTQKEYGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILK--SYDSWWVPFKTFIETKR----NVTDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPAS-RITATNSDFSY-------VKFSGPEEHVPGRRLVENLIQGKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEE-GDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTSSHDEDAAS 1026
               G+ L+  Y++L     + V  R  +   G + +GM +G +FG+   +G+ Y  +H I+PFL LGIG+DD+F+I++   NLS +E++  L E+I L ++H+GV+ITVTSLTD+ AF + + T +P L+AFC++ A+ I+ +Y    ++F+A  +LD+RRI + +       R  +  P  S WT   Y K ++     I     +++++ GI+L VFG GI+GT+ I+Q++DP+  +   SY  +++  +   +P  G   +IY G +   EN  E       ++D+L  + + ++  S+  +W  FK  I+       N +      +   F + L  F+F+ +    K  I + +E    Q     I ++ S F Y       V+    +E + G  L + + +   F S   S S  +  W  ++VIS EL RN++L   C+ I+  +LLAN+++ L+VL  + +T+ ++ GF +F GL I+ ++ + ++L++GL +DYSAHI   + +++ G R ER+  +L+ MGPAI +GG +TFLA V+L F++S+ F +F  +    V+FGL++ LV++P++LS  GP +  +    S
Sbjct:   44 FGVGFLLILIYSLLVTSDFHPVNQRVMLLLLGFMVIGMAIGSSFGLGFHIGIIYADLHPIIPFLLLGIGVDDLFIIIKSTDNLSQKEKELPLKERIALVVQHSGVSITVTSLTDLVAFLISSTTSIPLLRAFCLSVALGISFLYFFVVTFFIAWYTLDERRIEEGKAFLWFTKRVHSVPPKKSTWTHNLYKKYYVEYLFAIP----SQLILGGISLLVFGFGIFGTFNIQQDYDPITYMRQNSYQVKFIEAKNRLYPEFGDIVDIYVGNIPYWENIQEM-----KQIDSLVNNCQYVRTGSHSLYW--FKHVIDVCESEGFNCSTEFDFKKAIVFSLALKPFIFADL----KLNITILEEFQDFQDLKWHIQSSKSTFQYGHLESISVQTKAMDEIILG--LEKIIFEDSDFVSPI-SRSLAFHQWTANKVISKELIRNMSLNFACITIVLFMLLANIQLSLIVLFTVAMTITEIAGFAYFVGLNIEIVTSLELILSVGLVLDYSAHIGVCYTIQKTGSRRERSQRALLDMGPAIFSGGFSTFLAFVVLFFNDSYIFTSFCHMLGFGVLFGLWNALVFMPLLLSYFGPLTEEELKTVS 623          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000010537 (supercontig:LSalAtl2s:LSalAtl2s6:1415448:1420769:-1 gene:EMLSAG00000010537 transcript:EMLSAT00000010537 description:"maker-LSalAtl2s6-augustus-gene-13.12")

HSP 1 Score: 179.874 bits (455), Expect = 3.223e-46
Identity = 154/650 (23.69%), Postives = 294/650 (45.23%), Query Frame = 0

Query:  452 LMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRY--------RNWIPSAWT--------------QKEYGKM--FMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLED----IENDLEQFEKLSLEMDALTKDGKILKS-YDSWWVPFKTFIETKRNVTDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMV-CVFIITLVLLANVKICLMVLSC---ICLTLVDM----VGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIV--EEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTSSHDEDAASTAGGSSINTNSTN-EDNYKPSPLSAGLMNEGYVEDE 1061
            +M  ++++     +  + +  +   G+ S  +     FG    +G+ +  ++   PFL LGIG+DD FVI+  W   S      ++PE++G     A V+ITVTSLTD F+F  G VT  P ++ FC+   I++A IY    + F A+L++  R  +++  G   CI+         ++W+   +                K++  M  F     ++L + + K +VL +         +G   I +  +   +    SY  ++ ++ + +F    +  ++      +    + +D +   ++   + AL     I K+   SW   F  +I+   N  D   ++   + EM   D L +   S   P   L  ++  N   + I A+       +F     ++    + ++++   +     FS   +  +      I  + +  +A   V C+ + +LV++      +  L C   +  +++ +    +G++ +WG+ +D ++ IN+++ IG  VD+SAHI + +I   E    +ER  +SL  +G  I+ G  +T L ++ L F+ S+ F+TFFK+  L ++ G  HGL+ LPV+LSL GP S     +   A  S ++T ST      +  P S   +N GY   +
Sbjct:  282 IMVVFSLVSCLMSDATKSKPLVGFAGLFSATIATIAGFGFACYVGVEFISLNFAAPFLLLGIGVDDTFVILSSWRRSS---SNLSIPERMGRCYSEAAVSITVTSLTDFFSFMAGVVTPFPCVRIFCLYTGISVAFIYFWHLTLFGAILAVSGRAEKNNLHGMF-CIKVIPKSLAVNKSWLTRTFMTGGTNPKDPHNPDDNKDHAGMVFFRDNMGRLLSRTWFKSLVLLLFGAYIVVACWGVTNIEEGLEKQNMANYDSYSVKYYSMDDSYFREYSYTISVIITSASNERDLLYSDSKTQARIERVLYALENSXFIEKAATQSWLRDFLDYIKRNENYED---IILPVSTEMEFVDTLSNVYLS--DPSNPLRLDVAYNHNKNGIIAS-------RFLIQGRNILNSYMEQSMVAELRDICNQFSTPDISISVFHPYFIYIDQYLAIAPQTVQCILVTSLVMMIISFFLIPSLVCSFWVAFSIISIELGVIGYMTWWGVRLDGVALINLIMCIGFSVDFSAHICYHYITDTESSSPDERIRSSLYGLGLPIIQGATSTILGVLGLAFAPSYLFVTFFKMVFLVIVLGGLHGLIILPVLLSLFGPGSCRSRKSIVVA--SEVSTPSTTISCKMRKEPPSCYTVNLGYASSD 913          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000008727 (supercontig:LSalAtl2s:LSalAtl2s542:206164:232722:1 gene:EMLSAG00000008727 transcript:EMLSAT00000008727 description:"maker-LSalAtl2s542-augustus-gene-2.8")

HSP 1 Score: 175.252 bits (443), Expect = 6.037e-45
Identity = 143/584 (24.49%), Postives = 271/584 (46.40%), Query Frame = 0

Query:  467 VEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCI--------RYRNWI-----------PSAWTQKEYGK-----MFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANI-YTGPLE----DIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFSSKAFS-FSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGD--REERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTS 1018
            V+ +  +   G+IS  M    +FG     G+P+  I+   PFL LGIGIDD FV++  W   S  E   T+P ++  T + A ++IT+TS+TD+ +F +G +T  P ++ FC+     +A  Y+   ++F   +++     + +R     C+          +NW+              + +K+  +     +F    A+ +  G+ KI VL           +G   I++  +   L    SY  ++  +++ +F    +  N+  +G        ++ND+E+  K  LE  +           +SW+  F  +++  ++  +      E     F+S      +GS +   +   K++  +     I A+       K     +    ++ V+ L      S+   + F   +  ++   ++     + + +A   +  I+L+ + N    L V   I    V +VGF+  WG+ +D+IS IN+++ IG  VD+SAHI++ ++  E     +ER  +S+ ++G  I+ G I+T L +V L  + S+ FITFFK+  L ++ G  HGL+ LP++LS+ GP+S
Sbjct:  298 VKSKPLLGLFGVISAIMAXLFSFGFIIYCGVPFIGINLAAPFLMLGIGIDDTFVMLGSWRRTSVHE---TVPARLAQTYRDAAISITITSITDMLSFWIGIITPFPSVRIFCLYTGACVAFTYLWHITFFGGCMAIAGYAEKSNRHSLT-CLTVIPKSKAESKNWLYRIFCSGGISPDDPFNKKDNAENAIMVIFRDYVARAINNGYFKIFVLVFFTFYLVIAGWGVSNIQEGLERKKLARYDSYSVEFYEMEDTYFREYPYRVNVVLSGHFNYSNLQVQNDVEKLLK-KLENSSFVDHVYT----ESWYRDFLDYVKRNKDFAELDISTEEN----FISTLKEVYLGSGFTHYV---KDVHFSTDNKSIIASRFLIQGTKIYNTNDE---KKFVQELRYVCLTSNYNVTVFHPYFIYFDQFLMVLPTTIQCITVAAFVMMFISLIFIPNPVCSLWVAFSIISIEVGVVGFMTLWGVGLDSISMINLIMCIGFSVDFSAHISYHYMSCEKSFTDKERIRDSMYALGLPIVQGAISTILGVVGLALAPSYVFITFFKMVFLVIVLGALHGLLVLPILLSIFGPSS 862          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000012098 (supercontig:LSalAtl2s:LSalAtl2s867:155984:163315:1 gene:EMLSAG00000012098 transcript:EMLSAT00000012098 description:"maker-LSalAtl2s867-snap-gene-0.32")

HSP 1 Score: 140.584 bits (353), Expect = 4.436e-34
Identity = 136/608 (22.37%), Postives = 287/608 (47.20%), Query Frame = 0

Query:  470 RTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVG-AVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSL-----DQRRIRDHRDGFAPCIRYRNWIPSAWTQKEYGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQE-FDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANI-YTGPLEDIENDLEQFEKLSLEMDALTKDGKILKS---YDSWWVPFKTFIETKRNV-TDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFI-VEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTSSHDEDAASTAGGSSINTNSTNEDNYKPSPLSAGLMNEGYVEDELPK 1064
            + +++  G +++ + +G A+G    +G+ +  I+    F+ LG+G+DD++VI+  W   S   +  ++PE++ L  + A V+IT+TSLT++ +F +G A  + P +  FC+   I I  IYI   ++F  +L++     +  R++  +D         + + S          + +    IL K + K++V+ + L    + I       +E  D   L    S+L  +   ++ +F  N +   + +T  L+   + + Q  ++S  ++ +     + ++    ++W   F   +E K+ + T W     E  F   LS+ L       + P+I  E+ +V N   ++I A+       K     E     + +  ++    F  +   F+ ++  ++    +      ++++  + V +IT +L+ N+   L V   +    + ++GF+  + + +D IS I +++ +G  +DYSAH+++ F+ +   +   R  + L + GP I+   ++T L +  L F  S+   +F  + ++ ++ G+ HG V LPV+LSL    S+ D D  S      +  N  N  +  P  + + L++   +E+E+PK
Sbjct:  295 KPYVSLVGSLNIVVAIGAAWGFLMYIGVEWISINLASVFILLGVGLDDIYVILFSWRRTS---ELLSVPERLSLCYEEAAVSITITSLTNIVSFVMGSAFPVFPSVWIFCLYSGIGIFFIYIWTLTFFGGVLAISGYTEETERLKKKQD--------IDIVDSEIGFDFLNHFYESTLPNILNKVYAKVIVIALYLTYLTSFILIILNNTKEGLDRSKLSRYDSFLSSYYKAEDNYFRQNPYRFQVVFTEALDYSNSSVRQ--QISNFINKIQNSKYVSQNEQFKENWLHVFDQIVEKKQTIFTGWTLENSEHRFITKLSETL----HRHHLPEI--EENIVFNHNKTKILASRIFLQSEKVRNSHEEKLMMQDLYEILDSAPF--ETIIFNPLFPFFDQFLQVLPSTIYSVSITAIVVILITFILIPNIVFTLWVTIALVSIEIGVLGFMVIFEINLDIISMICLIMCVGFSIDYSAHVSYHFVSMNIRNNSIRLKSXLXAYGPPIVQSAVSTMLGVTGLFFLPSYIIRSFSLLLVIVILLGVIHGTVLLPVLLSLTFCNSNTD-DIQSIQSSPQLE-NQKNLSSAIPLIIPSNLVDPC-LENEIPK 878          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000004370 (supercontig:LSalAtl2s:LSalAtl2s22:157610:161978:1 gene:EMLSAG00000004370 transcript:EMLSAT00000004370 description:"maker-LSalAtl2s22-snap-gene-1.32")

HSP 1 Score: 114.005 bits (284), Expect = 9.117e-26
Identity = 138/598 (23.08%), Postives = 265/598 (44.31%), Query Frame = 0

Query:  442 DAVKMAAGYFLMFFYTMLFLGKL-NLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPI-HGILPFLALGIGIDDMFVIVQCWYN--LSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRNWI--PSAWTQKEYGKMFMAGFAK-----ILKK---GFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTMVREGNFEMFLSDFL-----FSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFSSKAFSFSKV----YAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGG--ITTFLALVLLGFSESHAFI-TFFKVFMLTVIFGLFHGLVYLPVILSL 1013
            D + +   YFLMF Y    L    N       +A  GI  V   +    G+   +G+  T I   I+PFL L +GID++FV+V       LS E ++  +   IG+T++    A+ +++LT   AF +G ++ +P +  F +    ++   ++ Q + F+A+LSLD +RI+  +     C+  +  I   +  ++  Y K     F K     ++KK    F+ +V++ ITL +    +  T  I    DP   +P  SY++++    ++++            P+  + N        ++++  +    +I  S       F+T ++      D  T+   G  + ++ D+         V S+ K  I+    +  +     +  T++DF        +     R L +   +    +S  +S   V    Y + +TD ++S      +A  ++ + +I   L   + I L  ++ +   L+ + GF+   G+ ++ I  +N+++A G+ V+       + I+ +   EE   +        IL  G  IT  + + +LGFS+S+ F   FF V++  V+ G  HGL+ LPV+LS 
Sbjct:  404 DVLVVIISYFLMFVYVSFALTDFRNKKYFGITLAIAGIFMVLGSILSTTGLFGFIGVKATMIIFEIVPFLVLAVGIDNIFVLVLTARRKLLSLEGREMEVDSLIGITLEEIAPAMLISTLTQTCAFYLGTLSKMPAVVTFALYAGTSLLINFLAQITGFIAILSLDLKRIKSKKLDLFCCLSDKGEIDVDNPKSRFNYQKTISTFFEKYVSPVLMKKLTVKFSVMVLIAITLSI---TLVFTLNIPVGLDPESTMPKGSYVKKYFEWLKLYYSVG--------PPVYFVVNS------TNMDLATVKSQNRICGSAGCDPYSFQTAMKLWSTKPDLTTLATSG--QSWIDDYFSWSNDCCMVDSQGKICILFSNFMAFHT----VLRTSNDF-------VQAIESTRILSDKFARENNITSFPYSIFHVYYEQYLSMKTDTLVS------IAYVLLTMLLICWFLSGKIVIGLYTVTFVSSILIHVTGFISMLGIQLNAILAVNLLVACGISVEICIGYLTSVIMGKRSAEEVYNDQ-----AGILMSGIHITNLIGVSVLGFSQSNIFFYYFFTVYISIVVIGFVHGLIVLPVLLSF 960          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. L. salmonis genes
Match: EMLSAG00000001950 (supercontig:LSalAtl2s:LSalAtl2s1345:18618:29195:1 gene:EMLSAG00000001950 transcript:EMLSAT00000001950 description:"maker-LSalAtl2s1345-snap-gene-0.7")

HSP 1 Score: 105.145 bits (261), Expect = 5.005e-23
Identity = 76/297 (25.59%), Postives = 142/297 (47.81%), Query Frame = 0

Query:  409 LDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLG-----KLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYT-PIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIR-YRNWIPSAWTQKEYGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVH 698
            L+ + E E+   + I     RS  D  ++  + D   +A  Y +MF Y    LG     K  +VE +  +  GG+I V + +  + G+   + +P T  I  I+PFL L +G D++F++VQ      P     T  E +G  +     ++ +TS+T+   F +GA++ +P ++AF +   +A+   +++Q + F++L+SLD  R  D+      C+R  +  +     +    K+F   +A  + K + +  V+ I  G F T +  T  I    D  L +P  S++ ++    E +
Sbjct:  558 LEKALEWEKAYYMDIAFTSERSIEDELAKETYGDISTIAVSYIIMFIYITFSLGENTTWKRFMVESKITLGLGGVIIVLLSVAASIGIFGFIDVPATLIIFEIIPFLVLAVGXDNIFIMVQTC-QRDPRGATETHVEHVGRIVGEVAPSMLLTSVTESTCFFLGAISDMPAVRAFALYAGMALLIDFLMQVTCFISLISLDMARQNDNYYDIICCVRGSKKNVRQNGGEGVVYKLFKHFYAPFVMKEWVRAAVVIIFFGWFCTSLAVTPRIEVGLDQKLSMPDDSFVLKYFTFIEKY 853          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|83305902|sp|O15118.2|NPC1_HUMAN (RecName: Full=NPC intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1 protein; Flags: Precursor)

HSP 1 Score: 196.823 bits (499), Expect = 6.483e-50
Identity = 171/688 (24.85%), Postives = 318/688 (46.22%), Query Frame = 0

Query:  401 EKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLN-----LVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIH-GILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIR-YRNWIPSAWTQKEYGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWL---------------AVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSY-DSW--WVPFKTFIETKRNVTD--------------WRTMVREGN-----------FEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHV----PGRRLVENLIQGKKFSSKA---FSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSC--ICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVE-EGDREERAMNSLVSMGPAILNG-GITTFLALVLLGFSESHAF-ITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTSSHDEDAAS 1026
            EKE I  +     ++ +   + I     RS  D  +     D   +   Y +MF Y  L LG +      LV+ +  +   GI+ V   +  + GV S +G+P T I   ++PFL L +G+D++F++VQ  Y      Q  TL +++G  +     ++ ++S ++  AF +GA++++P +  F +   +A+   ++LQ + FV+LL LD +R   +R     C+R   +      ++    + F   ++ +L K + + +V+ I +GV    I     +    D  L +P  SY+  +                 ++E H  T+  G N+  G +    + L Q    + ++D  T+ G    S+ D +  WV  ++      N+TD               R +  EG              MFLSD    K G            ++     +R+ AT     +       + +      R +  N+ +    +  A   F +S  Y  +E    I  +   NL +++  +F++T+VLL   ++   V+ C  I + LV+M G +  WG++++ +S +N+V++ G+ V++ +HI  AF V  +G R ERA  +L  MG ++ +G  +T F  +V+L F++S  F I +F++++  V+ G  HGL++LPV+LS +GP+ +  +  A+
Sbjct:  584 EKEFINFV-----KNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLVDSKVSLGIAGILIVLSSVACSLGVFSYIGLPLTLIVIEVIPFLVLAVGVDNIFILVQA-YQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGALSVMPAVHTFSLFAGLAVFIDFLLQITCFVSLLGLDIKRQEKNRLDIFCCVRGAEDGTSVQASESCLFRFFKNSYSPLLLKDWMRPIVIAIFVGVLSFSIAVLNKVDIGLDQSLSMPDDSYMVDYFKSISQYLHAGPPVYFVLEEGHDYTSSKGQNMVCGGMGCNNDSLVQQIFNAAQLDNYTRIGFAPSSWIDDYFDWVKPQSSCCRVDNITDQFCNASVVDPACVRCRPLTPEGKQRPQGGDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILLGH-GTRVGATYFMTYHTVLQTSADFIDALKKARLIASNVTETMGINGSAYRVFPYSVFYVFYEQYLTIIDDTIFNLGVSLGAIFLVTMVLLG-CELWSAVIMCATIAMVLVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTVSMKGSRVERAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKSCAT 1263          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|123778281|sp|Q0EEE2.1|PTHD3_MOUSE (RecName: Full=Patched domain-containing protein 3; AltName: Full=RND-type protein RNDEu-3)

HSP 1 Score: 194.897 bits (494), Expect = 8.427e-50
Identity = 148/586 (25.26%), Postives = 278/586 (47.44%), Query Frame = 0

Query:  449 GYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRR------IRDHRDGFAPCIRYRNWIPSAWTQKEYGK-------MFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDI---ENDLEQFEKLSLEMDALTKDGKILKSYDSWWV-PFKTFIETK-RNVTDWRTMVREGNFEMFLSDF-LFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVE-EGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLM 1014
             Y L+  + ++   +L+ + ++  +A  G+ SV M +   FG+   LG+P+  I    PFL LG+G+DDMF+++  W   S  E  R   E++  +     V+IT+T++T+V AF  G  +    +Q FC+     +   Y    + F A+++LD +R        +  D     ++    +P      ++G+        F   F   L     K +V+ + +    + IYG + +++  D   L    SY+  +  V+E +F  + +G  +     E +   +ND+ Q  KL   M    ++  + K+   +W+  +  ++ +   N  D  T +   N   FL  F +F+     Y   I    E+  ++   +I   +S  +  +       +  R + EN        ++AF +   YAA   + V      RN+ +A   +FI++L+L+ +    L V   I   +V + GF+ FW + +D+IS IN+V+ IG   D+SAHI++AF+   E    ++++ +L  +G  +L   I+T + + +L  ++++ F TFFK+  L + FG  HGL+++PV L+  
Sbjct:  346 AYILIILFAVVSCSRLDCIRNKMCVAVFGVFSVAMSVVSGFGLMLHLGVPFVIIVANSPFLILGVGVDDMFIMISAWQKTSLSESIR---ERLSNSYSKVAVSITITTITNVLAFYTGITSSFRSVQYFCIYTGTTLLFCYFYSITCFGAVMALDGKREVAWSRWLEKPDQKYSSLKKSCCVPFGSLIDKHGEDNHPMNLFFRDYFGPFLTTSKAKFIVVLLYIFYIISSIYGCFQVQEGLDLRNLASDDSYITPYFNVEEDYF--SDYGPRVMVIVTESVNYWDNDVRQ--KLDKCMTQFEENEYVDKNLTEFWLEAYMQYMNSSGNNPNDKNTFM--NNIAGFLQFFPIFT-----YDINISSSNEITSSRGFIQIVDVSSSSNKKRM-----LLKLRSIAENCEVPLMVYNQAFIYFDQYAAIIENTV------RNVMIASTAMFIVSLLLIPHPVCSLWVTFAIASVIVGVTGFMAFWNVNLDSISMINLVICIGFSFDFSAHISYAFVSSTEPSVNKKSIEALYLLGYPVLQSAISTIIGVCVLSAAKAYIFRTFFKIMFLVMFFGAAHGLIFIPVFLTFF 906          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|8134591|sp|P56941.1|NPC1_PIG (RecName: Full=NPC intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1 protein; Flags: Precursor)

HSP 1 Score: 187.578 bits (475), Expect = 6.130e-47
Identity = 169/684 (24.71%), Postives = 317/684 (46.35%), Query Frame = 0

Query:  414 ESEERSGVKIYAN--------VARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLN-----LVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIH-GILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIR-YRNWIPSAWTQKEYGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWL---------------AVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSY-DSW--WVPFKTFIETKRNVTD--------------WRTMVREGN-----------FEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGP--EEHVPGRRLVENLIQ--GKKFSS-KAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLL-----ANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVE-EGDREERAMNSLVSMGPAILNG-GITTFLALVLLGFSESHAF-ITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTSSHDEDAAS 1026
            ESE  + VK Y N          RS  D  +     D   +   Y +MF Y  + LG +      LV+ +  +   GI+ V   +  + G+ S +G+P T I   ++PFL L +G+D++F++VQ  Y      Q  TL +++G  +     ++ ++S ++  AF +G ++++P +  F +   +A+   ++LQ + FV+LL LD +R   +R     C++   +      ++    + F   +A +L K + + +V+ + +GV    I     +    D  L +P  SY+  +                 V+E H  T+  G N+  G L    + L Q    + ++D  T+ G    S+ D +  W+  ++      N TD               R +  EG              MFLSD    K G            ++ N      T   +  + ++ S    +     R +  N+ +  G + SS + F +S  Y  +E    +  +   NL +++  +F++T+VL+     A V +C+     I + LV+M G +  WG++++ +S +N+V++ G+ V++ +HI  AF +  +G R +RA  +L  MG ++ +G  +T F  +V+L F++S  F I +F++++  V+ G  HGL++LPV+LS +GP+ +  +  A+
Sbjct:  584 ESEFINFVKNYKNPNLTISFMAERSIEDELNRESNSDLFTILISYAIMFLYISIALGHIKSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYIGVPLTLIVIEVIPFLVLAVGVDNIFILVQT-YQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGGLSVVPAVHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNRLDVVCCVQGAEDGAGVQASESCLFRFFKNSYAPLLLKDWMRPIVIAVFVGVLSFSIAVLNKVEIGLDQSLSMPDDSYVMDYFQSLSRYLHAGPPVYFVVEEGHNYTSLKGQNMVCGGLGCNNDSLVQQIFTAAQLDNYTRIGFAPSSWIDDYFDWIKPQSSCCRVYNSTDQFCNASVVDPTCIRCRPLTSEGKQRPQGEDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILGNGSGVGATYFMTYHTVLQASADFIDAMQKARLIASNITRTMGLEASSYRVFPYSVFYVFYEQYLTVIDDTIFNLGVSLGAIFLVTVVLMGCELWATVIMCVT----IAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTLSTKGSRVDRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAIVLLGATHGLIFLPVLLSYIGPSINKAKSLAT 1262          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|449081276|sp|O35604.2|NPC1_MOUSE (RecName: Full=NPC intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1 protein; Flags: Precursor)

HSP 1 Score: 187.193 bits (474), Expect = 6.684e-47
Identity = 175/673 (26.00%), Postives = 315/673 (46.81%), Query Frame = 0

Query:  414 ESEERSGVKIYAN--------VARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLN-----LVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIH-GILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLD-QRRIRDHRDGFAPCIRYRNWIPSAWTQKEY-GKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSY-------LRQWL--------AVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSY-DSW--WVPFKTFIETKRNVTD--------------WRTMVREGN-----------FEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSG--PEEHVPGRRLVENL---IQGKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCI--CLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVE-EGDREERAMNSLVSMGPAILNG-GITTFLALVLLGFSESHAF-ITFFKVFMLTVIFGLFHGLVYLPVILSLMGPT 1017
            E E  S VK Y N          RS  D  +     D   +   Y +MF Y  L LG +      LV+ +  +   GI+ V   +  + G+ S +GMP T I   ++PFL L +G+D++F++VQ  Y      Q+ TL +++G  +      + ++S ++  AF  GA++ +P +  F +   +A+   ++LQ + FV+LL LD +R+ ++H D    C+R  +    +   + Y  + F   FA +L K + + +V+ + +GV    +     +    D  L +P  SY       L Q+L         ++E +  ++  G N+  G +    + L Q    + E+D  T+ G    S+ D +  WV  ++      NVT                R +  EG              MFLSD    K G            +V +      T   +  + +K S    +     R +  N+   ++ K    + F +S  Y  +E    I  +   NL++++  +F++TLV+L   ++   V+ CI   + LV+M G +  WG++++ +S +N+V++ G+ V++ +HI  AF +  +G R  RA  +L  MG ++ +G  +T F  +V+L F++S  F I +F++++  V+ G  HGL++LPV+LS +GP+
Sbjct:  584 EKEFISFVKNYKNPNLTISFTAERSIEDELNRESNSDVFTVIISYVVMFLYISLALGHIQSCSRLLVDSKISLGIAGILIVLSSVACSLGIFSYMGMPLTLIVIEVIPFLVLAVGVDNIFILVQT-YQRDERLQEETLDQQLGRILGEVAPTMFLSSFSETSAFFFGALSSMPAVHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNHLDILC-CVRGADDGQGSHASESYLFRFFKNYFAPLLLKDWLRPIVVAVFVGVLSFSVAVVNKVDIGLDQSLSMPNDSYVIDYFKSLAQYLHSGPPVYFVLEEGYNYSSRKGQNMVCGGMGCDNDSLVQQIFNAAELDTYTRVGFAPSSWIDDYFDWVSPQSSCCRLYNVTHQFCNASVMDPTCVRCRPLTPEGKQRPQGKEFMKFLPMFLSDNPNPKCGKGGHAAYGSAVNIVGDDTYIGATYFMTYHTILKTSADYTDAMKKARLIASNITETMRSKGSDYRVFPYSVFYVFYEQYLTIIDDTIFNLSVSLGSIFLVTLVVLG-CELWSAVIMCITIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTMSTKGSRVSRAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFEIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPS 1253          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|62906873|sp|Q03602.2|PTR9_CAEEL (RecName: Full=Patched-related protein 9)

HSP 1 Score: 134.035 bits (336), Expect = 1.249e-30
Identity = 133/577 (23.05%), Postives = 244/577 (42.29%), Query Frame = 0

Query:  456 YTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWY--NLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRNWIPSAWTQK-------EYGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKD--GKILKSYDSWWV-PFKTFIETKRNVTDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTSSH 1020
            + M     LN+V+ + +I    + +  M    +  +   L  P+ P+  I+PFL + IG D+MF++++ W     S  E+QR +       +  +  ++ +TSLTD  +F +G+++    ++ FC  CA+AI  +++ Q ++F A++SL  RR      G  P        P   TQ        ++  +      KIL    ++I V  I L      I+    +    D  LL P  SY+ + L  QE  F   G     +   L   EN   +   +   +  L +D  G    S   +W+ PF+     K+          E +F   L  FL  +   +++  I             +I A    F   K  G +   P    +  +++  +F+   F +   +   +      + +  ++  A++ +  I ++++      + +   I    + ++G L      +D IS I IV+++G  VDY  H    F+++  +R E+    L+ M   IL   ++T + + LL F  S+   TF       V  G+ HGL++LPV+L  + P S +
Sbjct:  278 FCMFACSSLNIVKSQPFIGFFAMFNATMATIASTSLLLYLQYPFLPLVFIMPFLVVSIGTDNMFLMLKSWRMTKKSSNEEQRYIH-----ALTESAASLFLTSLTDGLSFAIGSISDFHAVRVFCTYCAMAILFMFLFQVTFFNAVMSLCCRR---EVSGKHPVFCCYEITPPEETQVLEKQSNFDFSSIVAKHLFKILNPLPSRIGVFFIFLAYLFISIHFAIGLPLGLDLKLLAPDDSYVSKELEAQERLFADYGG----FCFALVRAENISLKDPMVRRNLIGLYRDLGGSEYSSPSEFWLDPFEKKNRGKK--------YSESDFSDELHTFLAKEPHLKFRNDIRFTM-------MGKIEAMKMMFRVRKL-GKDNDAPRAEYMRKVMENSQFN--GFVYDTSFLLVDQQMTTVYNVIVDVVSAILTMLSICVLMVPRPVSAMCIAFAILSVNIGVIGALAATNTRLDIISMITIVMSVGFSVDYVTHTTFHFVIQRDNRLEKC---LLVMTEPILQSALSTAIGVSLLSFVHSYIVRTFVNTVFFVVGLGILHGLIFLPVLLDTIVPDSEY 821          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|73921247|sp|Q6T3U3.1|NPCL1_RAT (RecName: Full=NPC1-like intracellular cholesterol transporter 1; AltName: Full=Niemann-Pick C1-like protein 1; Flags: Precursor)

HSP 1 Score: 113.235 bits (282), Expect = 5.218e-24
Identity = 83/272 (30.51%), Postives = 127/272 (46.69%), Query Frame = 0

Query:  429 RSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLN-----LVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYT-PIHGILPFLALGIGIDDMFVIVQCWYNLS--PEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRNWIPSAWTQKEYGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWL 692
            RS  D  +     D    A  Y ++F Y  L LG  +      VE +  +  GG+I V   +  A G  S LG+P +  I  ++PFL L +G D++F+ V  +  L   P EQ+      IG T+     ++ + SL++   F +GA+T +P ++ F +T  +AI   ++LQ + FVALLSLD +R    R     C   R   P    +    + F   +A  L   F + VV+ + L +FG  +Y    I    D  L LP  SYL  + 
Sbjct:  619 RSLEDEINRTTIQDLPVFAVSYIIVFLYISLALGSYSRCSRVAVESKATLGLGGVIVVLGAVLAAMGFYSYLGVPSSLVIIQVVPFLVLAVGADNIFIFVLEYQRLPRMPGEQREA---HIGRTLGSVAPSMLLCSLSEAICFFLGALTPMPAVRTFALTSGLAIILDFLLQMTAFVALLSLDSKRQEASRPDVLCCFSTRKLPPPKEKEGLLLRFFRKIYAPFLLHRFIRPVVMLLFLTLFGANLYLMCNINVGLDQELALPKDSYLIDYF 887          

HSP 2 Score: 81.2629 bits (199), Expect = 3.317e-14
Identity = 58/153 (37.91%), Postives = 97/153 (63.40%), Query Frame = 0

Query:  869 LALAMVCVFIITLVLLANVKIC--LMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVE-EGDREERAMNSLVSMGPAILNG-GITTFLALVLLGFSESHAF-ITFFKVFMLTVIFGLFHGLVYLPVILSLMGP 1016
            LAL  V  F++  +LL  + +C  ++ L  I + LVD +G +  WG++ + +S IN+V A+G+ V++ +HI  +F V  +  R ERA ++ V MG A+  G  +T F  +++LGF+++    I FF++ +L  + GL HGLV+LPV+LS +GP
Sbjct: 1117 LALCFVPTFVVCYLLLG-LDMCSGILNLLSIIMILVDTIGLMAVWGISYNAVSLINLVTAVGMSVEFVSHITRSFAVSTKPTRLERAKDATVFMGSAVFAGVAMTNFPGILILGFAQAQLIQIFFFRLNLLITLLGLLHGLVFLPVVLSYLGP 1268          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|1042851721|sp|H2L0G5.1|PTC3_CAEEL (RecName: Full=Protein patched homolog 3)

HSP 1 Score: 102.064 bits (253), Expect = 1.563e-20
Identity = 55/176 (31.25%), Postives = 101/176 (57.39%), Query Frame = 0

Query:  842 SSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFIT-FFKVFMLTVIFGLFHGLVYLPVILSLMGP 1016
            S  AF+F      WE    + + L++ + +  + VF +  +L+ N     +++  + +T +++ GF+   G+ ++ IS + ++ A+G+ V+++AH+  AF+   G  ++R  + L  M   + +G I+TFL +V+L FSE    +T FF    L V  G+F+GL  LPVIL+L+GP
Sbjct: 1127 SGIAFTF------WEQYLTLRWNLFQAICIIALAVFCVISILMFNPWAATLIMCIVVITTIELGGFMGLMGIKMNPISAVTLICAVGIGVEFTAHVELAFLTALGTIDQRLESCLQHMFVPVYHGAISTFLGVVMLVFSEFDFVVTYFFYTMTLLVALGVFNGLCVLPVILTLVGP 1296          

HSP 2 Score: 85.5001 bits (210), Expect = 1.781e-15
Identity = 58/207 (28.02%), Postives = 99/207 (47.83%), Query Frame = 0

Query:  418 RSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLN----LVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTP-IHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIR 619
            R   + +   + S  D+  E   F+ + +  GY LM  Y     G+       V+    +A  G+I V +      G  + LG+ +      ++PFL+LG+GIDDMF+++  +     E        +IG+ +K  G+++ +TS+ ++ AF  G V  +P L++FC   AI +A   I     F A++ +D RR R  +   A C R
Sbjct:  603 RERRQFHPLASTSIADMLEEFSQFNYIIIVIGYILMVIYAAFTQGRFQGWWLAVQSNVALAICGVILVTISSICGLGFATHLGINFNAATTQVVPFLSLGLGIDDMFLLLHNY----DEIINICNKNEIGVLLKETGMSVMLTSINNILAFISGYVLPIPALRSFCSQTAILLAFNLIFLMFIFPAMIGIDLRRQRKGKRDLAYCSR 805          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|6225890|sp|Q90693.1|PTC1_CHICK (RecName: Full=Protein patched homolog 1; Short=PTC; Short=PTC1)

HSP 1 Score: 100.908 bits (250), Expect = 3.408e-20
Identity = 75/300 (25.00%), Postives = 148/300 (49.33%), Query Frame = 0

Query:  349 LGAIERNSSGHIVAAKSALYSWTAAFDPDGELYEE-------GGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTP-IHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLP--EKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHR-DGF----APCI-RYRNWIPSAWTQKE 632
            +G   +NSSG +V+A+ AL +      P  ++YE          +    D A  +   +++  +EV+    +   + S  K+ +    + +DI         +++A+GY LM  Y  L + + +  + +  +   G++ V + +    G+ S +G+ +      +LPFLALG+G+DD+F++   +   S   Q + +P  ++ G  +K  G ++ +TS+++V AF + A+  +P L+AF +  A+ +   + +    F A+LS+D  R  D R D F    +PC+ R     P A+ + +
Sbjct:  343 IGGTVKNSSGKLVSAQ-ALQTMFQLMTPK-QMYEHFKGYEYVSHINWNEDKAAAILEAWQRMYVEVV---HQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCAKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAF---SETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVTRVIQIEPQAYAEND 634          

HSP 2 Score: 76.6406 bits (187), Expect = 8.232e-13
Identity = 49/165 (29.70%), Postives = 92/165 (55.76%), Query Frame = 0

Query:  855 WETDEVISFELWRNLALAMV--CVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFIT-FFKVFMLTVIFGLFHGLVYLPVILSLMGP 1016
            WE  + I    W  L++++V  C F++  + L N     +++  + L  V++ G +   G+ +  +  + ++ ++G+ V+++ HIA AF+   GD+  RA+ +L  M   +L+G ++T L +++L  SE    +  FF V  +  I G+ +GLV LPV+LS  GP
Sbjct: 1017 WE--QYIGLRHWLLLSISVVLACTFLVCALFLLNPWTAGIIVVVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHIALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGP 1179          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|160415977|sp|Q13635.2|PTC1_HUMAN (RecName: Full=Protein patched homolog 1; Short=PTC; Short=PTC1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.445e-19
Identity = 75/297 (25.25%), Postives = 146/297 (49.16%), Query Frame = 0

Query:  349 LGAIERNSSGHIVAAKSALYSWTAAFDPDGELYEE-------GGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTP-IHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLP--EKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHR-DGF----APCI-RYRNWIPSAWT 629
            +G   +NS+G +V+A  AL +      P  ++YE          +    D A  +   +++  +EV+    +   + S  K+ +    + +DI         +++A+GY LM  Y  L + + +  + +  +   G++ V + +    G+ S +G+ +      +LPFLALG+G+DD+F++   +   S   Q + +P  ++ G  +K  G ++ +TS+++V AF + A+  +P L+AF +  A+ +   + +    F A+LS+D  R  D R D F    +PC+ R     P A+T
Sbjct:  343 VGGTVKNSTGKLVSAH-ALQTMFQLMTPK-QMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVV---HQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAF---SETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYT 631          

HSP 2 Score: 85.8853 bits (211), Expect = 1.240e-15
Identity = 103/468 (22.01%), Postives = 199/468 (42.52%), Query Frame = 0

Query:  616 PCIRYRNWIPSAWTQKEYGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEK-LSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNV--TDWRT------MVREGNFEMFLSDFLFSKVGSRYKP--------KIILEKELVCNQPASRITAT----NSDFSY------VKFSGPE------EHVPGRRLVENLIQGKKFSSKAF-------------------------------SFSKVYAAWETDEVISFELWRNLALAMV--CVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFIT-FFKVFMLTVIFGLFHGLVYLPVILSLMGP 1016
            PC +   W  S++ +K Y        A  L K   K+VV+ + LG+ G  +YGT  +R   D   ++P ++    ++A Q  +F               +I++ L    +  S     + ++ K L     W   F+ +++  ++   +DW T        + G+ +  L+  L  + GSR KP        + +++ + + N  A  I  T    N   +Y      ++   PE      +++P  RL     +  +++   F                               S+   Y     ++ I    W  L +++V  C F++  V L N     +++  + L  V++ G +   G+ +  +  + ++ ++G+ V+++ H+A AF+   GD+  RA+ +L  M   +L+G ++T L +++L  SE    +  FF V  +  I G+ +GLV LPV+LS  GP
Sbjct:  726 PCTK---WTLSSFAEKHY--------APFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKM--WLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGP 1180          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. SwissProt
Match: gi|6225892|sp|Q61115.1|PTC1_MOUSE (RecName: Full=Protein patched homolog 1; Short=PTC; Short=PTC1)

HSP 1 Score: 97.0561 bits (240), Expect = 5.408e-19
Identity = 74/298 (24.83%), Postives = 145/298 (48.66%), Query Frame = 0

Query:  349 LGAIERNSSGHIVAAKSALYSWTAAFDPDGELYEE-------GGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTP-IHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLP--EKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHR-DGF----APCI-RYRNWIPSAWTQ 630
            +G   +N++G +V+A  AL +      P  ++YE          +    D A  +   +++  +EV+    +     S  K+      + +DI         +++A+GY LM  Y  L + + +  + +  +   G++ V + +    G+ S +G+ +      +LPFLALG+G+DD+F++   +   S   Q + +P  ++ G  +K  G ++ +TS+++V AF + A+  +P L+AF +  A+ +   + +    F A+LS+D  R  D R D F    +PC+ R     P A+T+
Sbjct:  329 VGGTVKNATGKLVSAH-ALQTMFQLMTPK-QMYEHFRGYDYVSHINWNEDRAAAILEAWQRTYVEVV---HQSVAPNSTQKVLPFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAF---SETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTE 618          

HSP 2 Score: 89.7373 bits (221), Expect = 1.031e-16
Identity = 105/468 (22.44%), Postives = 201/468 (42.95%), Query Frame = 0

Query:  616 PCIRYRNWIPSAWTQKEYGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEK-LSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNV--TDWRT------MVREGNFEMFLSDFLFSKVGSRYKP--------KIILEKELVCNQPASRITAT----NSDFSY------VKFSGPE------EHVPGRRLVENLIQGKKFSSKAF-------------------------------SFSKVYAAWETDEVISFELWRNLALAMV--CVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFIT-FFKVFMLTVIFGLFHGLVYLPVILSLMGP 1016
            PC +   W  S++ +K Y        A  L K   K+VV+ + LG+ G  +YGT  +R   D   ++P ++    ++A Q  +F               +I++ L    K  S     + ++ K L     W   F+ +++  ++   +DW T        + G+ +  L+  L  + GSR KP        + +++ + + N  A  I  T    N   +Y      ++   PE      +++P  RL     +  +++   F                               S+   Y     ++ IS   W  L++++V  C F++  V L N     +++  + L  V++ G +   G+ +  +  + ++ ++G+ V+++ H+A AF+   GD+  RAM +L  M   +L+G ++T L +++L  SE    +  FF V  +  + G+ +GLV LPV+LS  GP
Sbjct:  712 PCTK---WTLSSFAEKHY--------APFLLKPKAKVVVILLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHKSFSNVKYVMLEENKQLPQM--WLHYFRDWLQGLQDAFDSDWETGRIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLSISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTVLGVLNGLVLLPVLLSFFGP 1166          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1325287135|ref|XP_023328314.1| (Niemann-Pick C1-like protein 1 [Eurytemora affinis] >gi|1325287137|ref|XP_023328315.1| Niemann-Pick C1-like protein 1 [Eurytemora affinis] >gi|1325287139|ref|XP_023328316.1| Niemann-Pick C1-like protein 1 [Eurytemora affinis] >gi|1325287141|ref|XP_023328317.1| Niemann-Pick C1-like protein 1 [Eurytemora affinis])

HSP 1 Score: 930.243 bits (2403), Expect = 0.000e+0
Identity = 468/980 (47.76%), Postives = 655/980 (66.84%), Query Frame = 0

Query:   83 GGVEGS-HEWGMYVARHPILVIVVSLLITSLSGLGFLRFSSESRAEKLWIPSDSPYLENKAWLDKNYPQTRRNHLALFVAEKNILTPEGIMEMLIMHQKIHAISVNNKTYQDLCFKLPIANIFLTKRRRRRQAE--------DNSVSTLNPLNSTSAIVPALASSSNDSSGAGTNSSS--PSNATSS-------------EAEESSNREEKFLFN------------FGDDPSDDPQDT---LSPQIYCDLVSTLDEKCLENSLLEIWRYSESRVKRLTQEEILYAVNQVAESPWFGYRTDFSVYLGAIERNSSGHIVAAKSALYSWTAAFDPDGELYEEGGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLP--EKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRNWIPSAWTQKEYGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTSSHD 1021
            GG+E   + WG ++A HP  +++VSL +T+LSG+GF+RF  E +A  LWIP++SPY  N+ WLD +Y + RR+ L +F AE N+LTPE +++M  + + I+ I VN KT++D+C ++PIAN+F T  +RRR+ E        D ++S+   L+ T +I  AL      +    TN +   P    S              +A+ S+ RE+  L              +G   +  P+ +   L   I C +V+ L EKCL++SLLEIWRY E  +   TQ+EI+ AVN +  SPWF +R +++  LG I RNS+GHI+AAK A   W  A   D EL    G GVEL+LAD  +L +E++MI++ L+ S       GVK++ N A+SF D++++AIFFDA+KMA GY LMF YT+L LGKLN +E + ++A  GI+SVGMGL IA G++SA+G PYTP+H  LPFL LGIGIDDMFVIVQCW+N+  EE   TLP  E+IGLT++HAGVA+T+T+LTDVFAFGVGAVT +PGL++FCV  AI +A+IYILQ S+F A LSLD++RI+  RDG  PC  ++N+   AWT  +  +  ++ +  +L     KI ++  +L + G GI+G   IRQ+FDP+LLLPA SYLR W+++ +  +P NGW A +YTG L     ++E+ E ++ +   L  +   +   +SWW   KT+   ++N + W++      F M LSDFLFS+ G+ YK     +  L C +PA  I A+   FS+  F GPEEH+P +R+V++++     S  AF+  K+YAAWETDE+I +ELWRN+ LAM+CV ++TLVL+AN++ICL V++ + LTL D+VGFLHFWGLTID ISCI IVLA+GLCVDYS HI HA++V +G R E++M SL ++G A+ NGG TTFLALVLLGFSES  F+TFFKVF+LTV+FGLFHGL+ LPV+LS++GP S  D
Sbjct:   13 GGLEDIFYRWGKFLALHPYPIVLVSLALTALSGIGFIRFRQEHQANLLWIPAESPYNLNQDWLDVHYKKNRRDQLIIFSAE-NVLTPESLIQMYELQKAINQIEVNGKTFKDICTRVPIANLFHTDDKRRRRRETLVTEPIFDPTLSSDPILDPTLSIESALEDLEETAVKNETNYAEIWPDYYNSEEEEVEEANFWPDYDADYSNEREDVELVQVIKPKVRINYELYGKKNNTGPKKSIKQLPRDIKCGIVTRLPEKCLQSSLLEIWRYKEDLIYSATQQEIIDAVNLLETSPWFSFRMNYTSQLGGIVRNSTGHIIAAKGAQMYWNIAVPDDVELVHSQGSGVELELADATTLAWEEQMIQITLNIS-----HPGVKVFPNAAKSFADVSADAIFFDAIKMAIGYLLMFTYTVLILGKLNTLELKFYLAITGIVSVGMGLIIAVGLSSAMGYPYTPMHSALPFLCLGIGIDDMFVIVQCWFNMKKEESD-TLPLHEQIGLTLRHAGVAVTITTLTDVFAFGVGAVTRMPGLESFCVCTAIGLASIYILQISFFTAFLSLDEKRIKSGRDGLIPCFVHKNYTAPAWTNFDLSEYIVSKYEWLLNSWIYKICIILTSLSLLGFGIWGCVCIRQKFDPILLLPADSYLRSWVSMHDDLYPNNGWEAEMYTGRLN--HTNMEKMEAMTNKFQDLVDNKMYITGTNSWWRDLKTYAREQKNYSSWQSFANPEEFPMILSDFLFSESGTTYKGNFRWDGTLECGKPAPPIIASRFSFSFYIFDGPEEHIPAKRMVDSIVAEAGVSETAFTHVKIYAAWETDEIIGYELWRNIGLAMMCVALVTLVLIANIQICLFVVTSVALTLADIVGFLHFWGLTIDIISCITIVLAVGLCVDYSVHIGHAYLVAKGTRVEKSMASLRTIGSAVFNGGFTTFLALVLLGFSESQIFLTFFKVFVLTVVFGLFHGLILLPVVLSIVGPVSKED 983          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1061485453|gb|ODN02586.1| (Patched domain-containing protein 3, partial [Orchesella cincta])

HSP 1 Score: 751.895 bits (1940), Expect = 0.000e+0
Identity = 376/942 (39.92%), Postives = 589/942 (62.53%), Query Frame = 0

Query:   89 HEWGMYVARHPILVIVVSLLITSLSGLGFLRFSSESRAEKLWIPSDSPYLENKAWLDKNYPQTRRNHLALFVAEKNILTPEGIMEMLIMHQKIHAISVNNKTYQDLCFKLPIANIFLTKRRRRRQAEDNSVSTLNPLNSTSAIVPALASSSNDSSGAGTNSSSPSNATSSEAEESSNREEKFLFNFGDDPSDDPQDTLSPQIYCDLVSTLDEKCLENSLLEIWRYSESRVKRLTQEEILYAVN--QVAE--SPWFGYRTDFSVYLGAIERNSSGHIVAAKSALYSWTAAFDPD----GELYEEGGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRNWIPSAWTQKEYGKMFMAG-FAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETK--RNVTDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFS---SKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGP 1016
            + WG  +   P+  IV  +++T L G+G LRF+ E+R +KLWIP +S ++++  WL+KN+P   R H A+  AE ++L P+ +  +L +H K+  I  +   ++D+CFKLP+   F T +RR+R+  +                     ++N ++    N     +A   + +E +  E+ F          DP  +L   +YC++VS+LD++C E ++LE+W Y+ S + +LT+EEI+ A+N  Q+ +   P FG   +++  LG ++RN SG+I+ A++ +  W A  +      G++  + G G      DK +  +EKE+ +  L          GV IY NVARSF DI+   I  DA  +A G+ ++F Y +  LGK N VE R  ++  G+  VG+ + +++G+ SA  + Y P+H ILPFL LGIGIDDMFVI+QCW NL PE+ ++++PEK+GLT++HAGV+IT+TSLTD FAF +G  T+LP LQ+FCV  A+ I A YI QA++FVA L++DQ+RI   R+ F P I + ++ P     + + +  +   + K+L + F+K++V  +T GVFG  ++G  L+RQEF+P+  LP  SYL ++ A ++  FP  G    I    + D+ ++L + E L L+ +    +   +   D+W+ PFK ++ +   +++ + +   +E  F  +L  FLFS  G +Y+ +   +K L C + A  +  ++++F +  FSGPE+HVP    ++ +I+   F+   S  F +S  +A WETDE+I+ EL+RN+ LAM+CVF+ TLVL+AN++ C MVL C+ LTLVD+ G +HFW LTIDT+SCI++VLAIGLCVDY+AHIAHAF+   G R ER+  +LV++GPA+LNGG +TFLA +LL  S+SH F+TFFK+F L V++GLFHGLV+LPV+LS++GP
Sbjct:   72 YRWGYQIGLTPVPFIVACVILTVLCGIGLLRFTPENRPDKLWIPWNSEFVKDTEWLNKNFPSPFRFHYAIITAE-DVLQPKVLDVLLEIHNKVKEIEASTYKWKDICFKLPVIK-FDTNKRRKREVNN---------------------ANNSATLFNGNFDGFDSAFDFDDDEEATSEKPF----------DPSVSLPTDVYCNIVSSLDKECFELNMLELWNYNSSVINKLTKEEIVNAINHEQIRQVFYPVFGRPVNYATELGKLQRNESGYIIGAEAVMIRWFARLNRTAMNLGDVLNDEGTG---QAVDKYNFMWEKELSKFFL----SFRPPEGVNIYFNVARSFADISGSTIMGDASMLAVGFSIVFLYVIFMLGKFNFVEQRPLLSLAGLSCVGLAILVSYGICSAFKIIYGPVHNILPFLLLGIGIDDMFVIMQCWNNLRPEDMKKSIPEKLGLTLQHAGVSITITSLTDFFAFAIGGTTVLPALQSFCVYAALGIVATYIFQATFFVAWLAIDQKRIERRRNAFIPYIIHASFSPKTLMTRSFAQTAIEKIYCKVLFRKFSKVMVFLVTFGVFGVNVWGNILLRQEFNPIWFLPQDSYLFKYFAARDQFFPEKGEEGFILMNDV-DLNSNLPKLESLLLDFE----NSTFVHEIDAWYPPFKQYVNSNFGKDIPNQKLTNKE--FNKYLGKFLFSPTGGKYQSRFKFDKNLTCGKSAPAVMLSSANFKFNIFSGPEQHVPAMNNIKRIIREANFTPANSHTFIWSFAFAGWETDEIITDELYRNMGLAMICVFLTTLVLIANLQTCCMVLLCVILTLVDVGGSMHFWNLTIDTVSCIDLVLAIGLCVDYAAHIAHAFMTSVGTRNERSTKALVNIGPAVLNGGFSTFLAFILLANSKSHVFLTFFKIFFLVVVYGLFHGLVFLPVLLSIIGP 966          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1022768415|gb|KZS13150.1| (Niemann-Pick C1 protein [Daphnia magna])

HSP 1 Score: 713.761 bits (1841), Expect = 0.000e+0
Identity = 393/941 (41.76%), Postives = 579/941 (61.53%), Query Frame = 0

Query:   90 EWGMYVARHPILVIVVSLLITSLSGLGFLRFSSESRAEKLWIPSDSPYLENKAWLDKNYPQTRRNHLALFVAEKNILTPEGIMEMLIMHQKIHAIS-VNNKTYQDLCFKLPIANIFLTKRRRRRQAEDNSVSTLNPLNSTSAIVPALASSSNDSSGAGTNSSSPSNATSSEAEESSNREEKFLFNFGDDPSDDPQDTLSPQIYCDLVSTLDEKCLENSLLEIWRYSESRVKRLTQEEILYAVNQVAESPWFGYRTDFSVYLGAIERNSSGHIVAAKSAL--------YSWTAAFDPDGELYEEGGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRNWIPSAWTQKEYGKMFMAGF-AKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTM-VREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQP-ASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQ--GKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGP 1016
             WG+ VA++P   I+ S++I +   +G L F++E+R  KLWIP DS +++N  WL +N+P   R   +  V + N+L PE ++ ML +H  I A+S  +   ++D+CFK+P+     T +RR+RQ                     L  S ND       +    +A   E   SS            DP  +P   L   I+CD+V  L+E CLEN++L+IW Y +  + +L++E+I+  +N V +S     R  +S  LG    N +GHIV+A S L        YS  A    D +     G G   ++ D ++L++EK  + +L + +         K+Y   ARSF DI+S  I  D   +AAG  ++F Y  L LGK NLVE R +++  GI SV M + +++G+ SA G+ Y P+H ILPFL LGIGIDDMFVIVQCW NL+ EE +R+LP +IG+TMKHAGV+IT+TS+TD  AF VGA T+LP L++FC+  A+ I A YI QAS+FVA L+LDQ+RI  HRDG  PC  +RNW PS+ ++ E  ++F   F AK L K   K+ +L +   +FG   +G+ L+RQEF+P+  +P  +YL Q+ +V E HFP NG  A+IY     ++ ++L++ + L   +D +  +  I+   D W+  FK F   KR+  DW    + +  F  +L ++LF++ G++++     E  L C    A  I+ TN DF +  FSG EEH+P  R ++++ +   ++F S+ F+ ++++++WETDEVI  EL+RNLA+AMVCVFI T +L+AN+  C++VL C+ LTLV + G +HFWGLTIDT+SCIN+VLAIGLCVDY+AH+AH F+ + G R ERA  ++ S+GPA+ +GG +TFLA + L  S+SH FITFFK+F+L V +GLFHGL++ PVILSL+GP
Sbjct:   40 RWGITVAKNPWTYIIASVVIAATCSIGLLNFTAENRPTKLWIPQDSGFVQNSEWLQENFPNEIRLQ-SWVVVDDNVLRPEVLLWMLELHDNITAVSSASGYQWRDVCFKVPVVTEQQTNQRRKRQ---------------------LNISENDPFDFEDFNFDGFDANFEEMPSSS-----------IDPDFEPAIDLPTGIFCDIVEGLEEACLENNILDIWSYKKEVISKLSREDIINTINTVQKSNL--TRVQYSSLLGQPRFNETGHIVSAGSLLVQLFSRVNYSLIANDVTDND----AGTG---EVVDPINLEWEKAFVNLLHNFTSPPNSS---KLYYMAARSFGDISSATILGDVRLLAAGICVVFIYVQLMLGKFNLVEQRAFLSLTGIASVAMAIVVSYGLCSAFGVFYGPVHSILPFLLLGIGIDDMFVIVQCWSNLNKEEMKRSLPVRIGITMKHAGVSITITSVTDFAAFAVGATTVLPSLRSFCIYSAVGILATYIFQASFFVAWLTLDQKRIESHRDGMIPCFVHRNWKPSSLSRIEPLQLFFNRFLAKYLFKWPAKVFILVLAAFLFGINTWGSVLMRQEFNPLWFIPTSTYLSQYFSVIESHFPDNGQMASIYIQT-TNLSSNLDRLDAL---IDTVRNETNIVSQVDDWFGGFKEF-TAKRHAIDWMNQNMTDEQFSTYLKNYLFTQKGAKFRRNFKFEDPLQCRSAIAPPISITNFDFVFRPFSGREEHIPAMRRIKDIAEDFSQQFGSQVFASARIFSSWETDEVIMEELYRNLAIAMVCVFITTFILIANLFACVLVLLCVVLTLVCVNGTMHFWGLTIDTVSCINLVLAIGLCVDYAAHVAHTFMTKTGTRNERAAATVSSIGPAVFHGGFSTFLAFIFLANSDSHVFITFFKIFVLVVAYGLFHGLLFFPVILSLIGP 930          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|321463817|gb|EFX74830.1| (hypothetical protein DAPPUDRAFT_306990 [Daphnia pulex])

HSP 1 Score: 706.057 bits (1821), Expect = 0.000e+0
Identity = 382/936 (40.81%), Postives = 571/936 (61.00%), Query Frame = 0

Query:   91 WGMYVARHPILVIVVSLLITSLSGLGFLRFSSESRAEKLWIPSDSPYLENKAWLDKNYPQTRRNHLALFVAEKNILTPEGIMEMLIMHQKIHAI-SVNNKTYQDLCFKLPIANIFLTKRRRRRQAEDNSVSTLNPLNSTSAIVPALASSSNDSSGAGTNSSSPSNATSSEAEESSNREEKFLFNFGDDPSDDPQDTLSPQIYCDLVSTLDEKCLENSLLEIWRYSESRVKRLTQEEILYAVNQVAESPWFGYRTDFSVYLGAIERNSSGHIVAAKSALYSWTAAFD----PDGELYEEGGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRNWIPSAWTQKEYGKMFMAGF-AKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTM-VREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQP-ASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQ--GKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGP 1016
            WG+ VA++P   I+ S++I +   +G L F++E+R  KLWIP DS ++ N  WL +++P   R    + V + ++L PE  + ML +H  I  + S N   ++D+CFK+P+     TKRRR+RQA            ++S I        +           P+ A                     DP+ +P   L   I+CD+V  L+E CLEN+ L+IW Y +  + +L++++I+ A+N V  S     R  +S  +G    N SGHIV A S L       +     +     + G G   DL D +SL++E   + +L + +      +   +Y   +RSF DI+S  I  D   ++AG  ++F Y  L LGK NLVE R +++  GI SV M + +++G+ SA G+ Y P+H ILPFL LGIGIDDMFVIVQCW NL+ EE +R+LP +IG+TMKHAGV+IT+TS+TD  AF VGA T+LP L++FC+  A+ I A Y+ QAS+FVA L+LDQRRI  HRDG  PC  +RNW PS  ++ E  ++F + F AK L K   K+ +L ++  + G   +G+ L+RQEF+P+  +P  +YL Q+ +  E H+P NG  A+IY     ++ ++L+Q E L   +D +  + +I+   D W+  FK F   KR+  DW +  + +  F  +L ++LF++ G++++     EK L C    A  I+ TN DF +  FSG EEH+P  R ++ + +    +F S  F+ ++++++WETDEVI  EL+RNLA+AMVCVF+ T +L+AN+  CL+VL C+ LTL+ + G +HFWGLTIDT+SCIN+VLAIGLCVDY+AH+AH F+ + G R ERA  ++ S+GPA+ +GG +TFLA + L  S+SH F+TFFK+F+L V +GLFHGL++ PV+LSL+GP
Sbjct:   66 WGITVAKNPWTYIIASVVIAATCSIGLLNFTAENRPTKLWIPQDSGFVHNSEWLQEHFPNEIRLQSWVLV-DDDVLRPETFLWMLELHDNITELASSNGYKWKDVCFKIPVVTEPETKRRRKRQA------------NSSEIESFGFEDFDFDGFDSNFEDIPAPAL--------------------DPNFEPAIDLPIGIFCDIVEGLEEACLENNFLDIWNYKKEVISKLSRQDIINAINSVERSNL--TRVQYSNLIGQPRYNESGHIVGAGSVLVQLLTRVNYSLITNDATSNDAGTG---DLVDPISLEWEGAFVNLLHNFTVT---LNNSNLYYMASRSFGDISSATILGDVRLLSAGICVVFIYVQLMLGKFNLVEQRAFLSLTGIASVAMAILVSYGLCSAFGVFYGPVHSILPFLLLGIGIDDMFVIVQCWSNLNKEEMKRSLPVRIGITMKHAGVSITITSVTDFAAFAVGATTVLPSLRSFCIYSAVGILATYLFQASFFVAWLTLDQRRIESHRDGMLPCFVHRNWKPSTLSRIEPLQVFFSRFLAKYLFKWPAKVFILILSAFLLGVNTWGSVLMRQEFNPLWFIPTSTYLSQYFSTIESHYPDNGQLASIYIQT-TNLSSNLDQLEAL---IDTVRNETQIVSQVDDWFTGFKDF-TAKRHAIDWSSQNMTDEQFSTYLKNYLFTQKGAKFRSNFKFEKSLECRSSIAPPISITNFDFVFRPFSGREEHIPAMRRIKEIAEEYSLEFGSHVFASARIFSSWETDEVIMEELYRNLAIAMVCVFVTTFILIANLFACLLVLLCVVLTLICVNGSMHFWGLTIDTVSCINLVLAIGLCVDYAAHVAHTFMTKTGTRNERAAATISSIGPAVFHGGFSTFLAFIFLANSDSHVFLTFFKIFVLVVAYGLFHGLLFFPVVLSLVGP 955          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1325261885|ref|XP_023331667.1| (patched domain-containing protein 3-like isoform X1 [Eurytemora affinis] >gi|1325261897|ref|XP_023331674.1| patched domain-containing protein 3-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 672.159 bits (1733), Expect = 0.000e+0
Identity = 371/949 (39.09%), Postives = 554/949 (58.38%), Query Frame = 0

Query:   89 HEWGMYVARHPILVIVVSLLITSLSGLGFLRFSSESRAEKLWIPSDSPYLENKAWLDKNYPQTRRNHLALFVAEKNILTPEGIMEMLIMHQKIHAI-SVNNKTYQDLCFKLPIA------NIFLTKRRRRRQAEDNSVSTLNPLNSTSAIVPALASSSNDSSGAGTNSSSPSNATSSEAEESSNREEKFLFNFGDDPSDDPQDTLSPQIYCDLVSTLDEKCLENSLLEIW-------RYSESRVKRLTQEEILYAVNQVAESPWFGYRTDFSVYLGAIERNSSGHIVAAKSALYSWTAAFD-PDGELYEEGGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRNWIPSAWTQKEYGKM--FMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTSSH 1020
              +G +VARHP++VI+  + +T + G G   F +E+   KLWIP +S Y  N  W+ +NYP+  R + +L +  +N+LT + +  M  + + I  + ++NN+T+ D C K P+       +IF  KRR  +  +D      +    T    P                                   K    F  D   DP        YC +V+ +   C+E S+LE++         ++  ++ LT E +L  +N    S  F    +F+ YL  IE N+ G I+ AK+    W    +  + +L    G G  + ++    L +E +M+EV+L+   +S+   G++ + NVARSF DI    I  D     AGY +MF Y  + LGK + VEHRT+++  GI+ V MG+ +++G+ SA G  Y P+H ++PFL LGIGIDDMFVI+QCW  LS EE++  L ++ G  MK AG AITVTS TDV AFGVGA TILP LQ+FC+  A+ I A ++ Q+S+F+A ++LDQRRI  +R+G  PC  Y +   +  TQ+  G +      +A+ L K   KI VL + L + G G+YG  L+RQEFDP   LP  +Y+ QW    +++FP++G    IY    E +  D+++ EKLS +   L +   I+   D+W  P+  +++ K N T+  + + +  F   LS FLFS VG+ Y+     +++L C + AS +  +   +++   +GPEEHVP    V+N+I+    S K F+ +  YA WETDE+IS E++RN+ALA+VCVFI TL+ ++N+   L++L C+CLTLVD+ GF+HFWGLTIDT+SC N+++AIGLCVDYSAH+ H F+ E+G RE+R + ++ ++GPA+ NGG++TFLA VLL  S SH F +FFK+F L V FGLFHGLV LPVILS +GP S+H
Sbjct:   33 QSYGEFVARHPVIVILSCVALTCVCGGGLYWFRAENEGVKLWIPYNSDYRLNNDWMFENYPRDLRFN-SLILTGENVLTAQVLRTMYRVSKSIRTMRNINNETWDDHCMKRPVIKVPSLFDIFGRKRREIKFDDDLFGDEFDEEKDTVITDPL----------------------------------KLATQFSIDEYPDP--------YCGIVTGMPLACMELSILELFGQGGDYDERTDDVMETLTDEIVLDVINNKNVSGIFLTPYNFTSYLSNIEYNTQGEIIGAKATYIIWLGRMNMTEAKLNPAPGRGEPISMS---MLQWEGDMLEVMLN---KSDYPKGLESFPNVARSFGDIAGSTIIGDITFFVAGYIMMFTYVQIMLGKFSCVEHRTYLSTVGILGVIMGIIVSYGICSAAGHFYGPMHSVMPFLMLGIGIDDMFVIIQCWDTLSEEEKKENLVKRFGFLMKQAGAAITVTSATDVIAFGVGAFTILPALQSFCIYAAVGIVATFVFQSSFFLAWMNLDQRRIESNRNGCCPC--YVHPETNNQTQERKGALQRVFKKYAEYLIKTPVKISVLLVALVLTGFGVYGNVLLRQEFDPTWFLPQDTYIAQWFVNNKMYFPSSGERGTIYFSGTE-LPADIDKIEKLSQD---LREQTDIIVKSDAWTRPYIDYLQ-KLNYTEKPSKLNKTAFRSSLSQFLFSPVGAVYRNGFNFKEQLECGELASEVQLSEITYTHRLMNGPEEHVPAMNRVKNIIESANISGKVFAMAMGYATWETDEIISLEIYRNMALAIVCVFITTLLFISNLGGSLLILVCVCLTLVDVGGFMHFWGLTIDTVSCNNLIIAIGLCVDYSAHVMHRFLTEQGPREDRVVQTVSNIGPAVFNGGVSTFLAFVLLATSRSHVFSSFFKIFFLVVSFGLFHGLVVLPVILSFIGPDSAH 925          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1325261904|ref|XP_023331682.1| (patched domain-containing protein 3-like isoform X3 [Eurytemora affinis])

HSP 1 Score: 671.389 bits (1731), Expect = 0.000e+0
Identity = 370/946 (39.11%), Postives = 557/946 (58.88%), Query Frame = 0

Query:   89 HEWGMYVARHPILVIVVSLLITSLSGLGFLRFSSESRAEKLWIPSDSPYLENKAWLDKNYPQTRRNHLALFVAEKNILTPEGIMEMLIMHQKIHAI-SVNNKTYQDLCFKLPIANI---FLTKRRRRRQAEDNSVSTLNPLNSTSAIVPALASSSNDSSGAGTNSSSPSNATSSEAEESSNREEKFLFNFGDDPSDDPQDTLSPQIYCDLVSTLDEKCLENSLLEIW-------RYSESRVKRLTQEEILYAVNQVAESPWFGYRTDFSVYLGAIERNSSGHIVAAKSALYSWTAAFD-PDGELYEEGGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRNWIPSAWTQKEYGKM--FMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTSSH 1020
              +G +VARHP++VI+  + +T + G G   F +E+   KLWIP +S Y  N  W+ +NYP+  R + +L +  +N+LT + +  M  + + I  + ++NN+T+ D C K P+  +   F    R+RR+ + +                      +D  G         +    E +       K    F  D   DP        YC +V+ +   C+E S+LE++         ++  ++ LT E +L  +N    S  F    +F+ YL  IE N+ G I+ AK+    W    +  + +L    G G  + ++    L +E +M+EV+L+   +S+   G++ + NVARSF DI    I  D     AGY +MF Y  + LGK + VEHRT+++  GI+ V MG+ +++G+ SA G  Y P+H ++PFL LGIGIDDMFVI+QCW  LS EE++  L ++ G  MK AG AITVTS TDV AFGVGA TILP LQ+FC+  A+ I A ++ Q+S+F+A ++LDQRRI  +R+G  PC  Y +   +  TQ+  G +      +A+ L K   KI VL + L + G G+YG  L+RQEFDP   LP  +Y+ QW    +++FP++G    IY    E +  D+++ EKLS +   L +   I+   D+W  P+  +++ K N T+  + + +  F   LS FLFS VG+ Y+     +++L C + AS +  +   +++   +GPEEHVP    V+N+I+    S K F+ +  YA WETDE+IS E++RN+ALA+VCVFI TL+ ++N+   L++L C+CLTLVD+ GF+HFWGLTIDT+SC N+++AIGLCVDYSAH+ H F+ E+G RE+R + ++ ++GPA+ NGG++TFLA VLL  S SH F +FFK+F L V FGLFHGLV LPVILS +GP S+H
Sbjct:   33 QSYGEFVARHPVIVILSCVALTCVCGGGLYWFRAENEGVKLWIPYNSDYRLNNDWMFENYPRDLRFN-SLILTGENVLTAQVLRTMYRVSKSIRTMRNINNETWDDHCMKRPVIKVPSLFDIFGRKRREIKFD----------------------DDLFG---------DEFDEEKDTVITDPLKLATQFSIDEYPDP--------YCGIVTGMPLACMELSILELFGQGGDYDERTDDVMETLTDEIVLDVINNKNVSGIFLTPYNFTSYLSNIEYNTQGEIIGAKATYIIWLGRMNMTEAKLNPAPGRGEPISMS---MLQWEGDMLEVMLN---KSDYPKGLESFPNVARSFGDIAGSTIIGDITFFVAGYIMMFTYVQIMLGKFSCVEHRTYLSTVGILGVIMGIIVSYGICSAAGHFYGPMHSVMPFLMLGIGIDDMFVIIQCWDTLSEEEKKENLVKRFGFLMKQAGAAITVTSATDVIAFGVGAFTILPALQSFCIYAAVGIVATFVFQSSFFLAWMNLDQRRIESNRNGCCPC--YVHPETNNQTQERKGALQRVFKKYAEYLIKTPVKISVLLVALVLTGFGVYGNVLLRQEFDPTWFLPQDTYIAQWFVNNKMYFPSSGERGTIYFSGTE-LPADIDKIEKLSQD---LREQTDIIVKSDAWTRPYIDYLQ-KLNYTEKPSKLNKTAFRSSLSQFLFSPVGAVYRNGFNFKEQLECGELASEVQLSEITYTHRLMNGPEEHVPAMNRVKNIIESANISGKVFAMAMGYATWETDEIISLEIYRNMALAIVCVFITTLLFISNLGGSLLILVCVCLTLVDVGGFMHFWGLTIDTVSCNNLIIAIGLCVDYSAHVMHRFLTEQGPREDRVVQTVSNIGPAVFNGGVSTFLAFVLLATSRSHVFSSFFKIFFLVVSFGLFHGLVVLPVILSFIGPDSAH 925          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1228374474|ref|XP_021948457.1| (protein patched homolog 2-like [Folsomia candida])

HSP 1 Score: 662.529 bits (1708), Expect = 0.000e+0
Identity = 363/947 (38.33%), Postives = 563/947 (59.45%), Query Frame = 0

Query:   89 HEWGMYVARHPILVIVVSLLITSLSGLGFLRFSSESRAEKLWIPSDSPYLENKAWLDKNYPQTRRNHLALFVAEKNILTPEGIMEMLIMHQKIHAISVNN-KTYQDLCFKLPIANIFLTKRRRRR--QAEDNSVSTLNPLNSTSAIVPALASSSNDSSGAGTNSSSPSNATSSEAEESSNREEKFLFNFGDDPSDDPQDTLSPQIYCDLVSTLDEKCLENSLLEIWRYSESRVKRLTQEEILYAVNQVAESPWFGYRTDFSVYLGA-IERNSSGHIVAAKSALYSWTAAFDPDGELYEEGGVGVEL--DLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNL--SP--EEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRN--WIPSAWTQKEYGK-MFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKF------SSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGP 1016
            + WG +VA HP+  I   LLITS+ GLG   F+ E+R +KLWIP +S + +N  W+   +P   R   A+  A+ N+L PE +  +L +H+++  I V   K ++ LC+KLPI   FL+KR+RR   QA  +S       NST+     L   SND   A  +     N+T       S   + F+              +  + YC +V+ LD  C E S  E+W + +S +  LTQEEI+  VN    SP FG   +++   G  I RN  G +++A S L  W+   +    L +EG V  +   ++AD   L++E + I+++ + SK+     G+  Y   ++S+ +I+   I  D +++A G+ +MFFY  + LGK N+VE R  +   G+  VG+   +++G+ S   + Y P++ ILPFL LGIGIDDMFVI+QCW NL  SP   + + ++PE++G+ MKHAGV+ITVTSLTDV AFGVGA TI+PGLQ+FC+  A+ I A+++LQA++FVA  +LDQ+R+ D R+ F   I+Y+   +IP++W+Q+ Y + +F    AK L   + KI V+ ITL + G  +YG  L+RQEFDP+  LP+ SY+ +++  +E ++P  G+ + +    +    N  E F+     ++ L K    + + ++W+  FK +     +       +    F+ ++  FL+S  GS+++P    +    C +    +      F + KF+GP+EH+P  R V++L++          S K F       AWETD +I+ EL+RN++L+++CV + T +LLAN +IC     C+ LTL+++ GF+HFWGL+IDT+S I+IVLAIGLCVDY+ HIA AF+   G RE RA  +L ++GPA+LNGG +TFLA++LL  S+SHAF +FFK+F + ++ GLFHGL++LPV+LS+ GP
Sbjct:   52 YRWGFFVATHPLKAISCCLLITSICGLGIFNFTMENRPDKLWIPRNSEFAQNTEWIRATFPSPIRTSFAIVTAD-NVLVPEVMRVILNIHERVKQIEVTGGKNWESLCYKLPITK-FLSKRKRRSIGQAYLHS-------NSTTNSHTGLQILSNDFDDAFDDEFFSENSTLKPQTSESGFSQLFV--------------VPDEFYCSIVNELDAACWEVSPAELWSFDKSLIDNLTQEEIIRVVNHELISPIFGRSVNYTAEFGGNIVRNDLGEVISAGSVLLRWSIGIN--QTLLQEGRVVTDKTGEVADDDCLEWELKWIDLMKNISKDMPP--GINTYFVNSQSWMEISEATILDDGMQLAMGFIIMFFYVGIMLGKFNVVEQRPLLTIAGLSCVGLACVVSYGLCSLCFIVYGPVNKILPFLLLGIGIDDMFVIMQCWNNLFSSPLTNKSKLSIPERMGMAMKHAGVSITVTSLTDVAAFGVGASTIMPGLQSFCIYAAVGIIAVFLLQATFFVACFALDQKRVEDRRNAFFLWIKYKEDEFIPNSWSQESYLQTLFNRILAKYLFTPWAKITVILITLSMLGVTMYGNTLLRQEFDPMWFLPSDSYMSKYVEKRETYYPGTGYPSFLVANQV----NWSESFQSFDRVLNDL-KSSDAIYNLENWYDDFKEYSNANFDTNIPNRPLTPSKFQKYMGKFLYSPAGSKHRPYFKSDGNFSCGESLPPLKTVVMPFRFRKFNGPQEHIPALRKVKSLLKTANMTFNHHESPKFFVTGTGLVAWETDNIINAELYRNVSLSLLCVLVTTFLLLANFRICAFCGICVVLTLINVGGFMHFWGLSIDTVSTIDIVLAIGLCVDYATHIALAFMTCTGSRESRAKQALKNIGPAVLNGGFSTFLAIILLAGSKSHAFQSFFKIFSVVIVCGLFHGLIFLPVLLSIFGP 966          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1061478937|gb|ODM96499.1| (Niemann-Pick C1 protein [Orchesella cincta])

HSP 1 Score: 651.744 bits (1680), Expect = 0.000e+0
Identity = 368/987 (37.28%), Postives = 558/987 (56.53%), Query Frame = 0

Query:   89 HEWGMYVARHPILVIVVSLLITSLSGLGFLRFSSESRAEKLWIPSDSPYLENKAWLDKNYPQTRRNHLALFVAEKNILTPEGIMEMLI-MHQKIHAISV-NNKTYQDLCFKLPIANIFLTKRRRRRQAEDNSVSTLNPLNSTSAIVPALASSSNDSSGAGTNSSSPSNATSSEAEESSNREEKFLFNFGDDPSDDPQDTLSPQIYCDLVSTLDEKCLENSLLEIWRYSESRVKRLTQEEILYAVNQVAESPWFGYRTDFSVYLGA-IERNSSGHIVAAKSALYSWTAAFDPD----GELYEEGGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPE----EQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRN--WIPSAWTQKEYGKMFM-AGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFS------SKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGP----------TSSHDEDAASTAGGSSINTNSTNEDNYKP 1045
            + WG  VA +P   I V LL T++ GLG + F+ E+R +KLWIP DS +  N  WL  N+P   R    +  A+ N+L P GIM+M++ +H+ +  ISV +NK ++D+CF+LPI    L         E       +P NS+     A   S+        +    S+A+++   +            GD  S DP  +L   IYC ++  LD+ C E + LE+W Y+ + V+ LT+EEI+   N    SP FG   +++  LG  IERNS G I+ A+S    W  A +      G    + G G   ++AD+  L +E E I ++   +K+  +  GV +Y    RSF DI+   IF D + + AG+F+MF Y    LGK NLVE R            +   I++GV SA  + Y P++ ILPFL LGIG+DDMFVIVQCW NL P+      + ++PE++G+ +KHAGV+ITVTSLTD+ AFGVGA TILPGLQ+FCV  AI I AI++LQ+++FVA  +LDQ+R+ + R+ +  CI+YR+  +IP++++Q+ Y ++F    F+K L     K +V+ +TLG   T IYG   +RQEFDP+  LP  S+L Q+L  +EV +P  G+   +    +    N    F ++   M        I + +D+W+  FK +             +    F+  +  FL+S  GS+++    +     C      +      F +  F GPEEH+PG   V+ L++    +         F++S  +  WE+DE+I+ EL+R++AL++ CV I    LLAN+++CL+   C+  T++++ GF++FW LTI+ +SCI+IVLAIGLCVDY++HI  AF+   G R  RA  +L ++GPA+LNGG +TF+++ LL  S+S AFI+F+K+F + V FGLFHGL++LPVILS++GP          T  H ++   T    +I+T S   +   P
Sbjct:   43 YRWGFIVATYPYRFISVCLLFTAICGLGLVNFTLENRPDKLWIPRDSAFAHNTEWLRDNFPSPVRQSHVIITAD-NVLDP-GIMKMILEIHESVKNISVFDNKKWEDICFRLPITKFDL---------ERRRRRRRSPTNSSHHEYDAFFGSAFSGFDEFDSEFFTSDASTNNTTKG-----------GDVRSFDPMLSLPEDIYCSIIDNLDDACWEINPLELWSYNRTEVESLTKEEIIQVFNTRLISPIFGRVVNYTADLGGNIERNSLGQIIKAESISLKWFVAINQSEVRAGRAVPDMGTG---EVADETGLAWETEFIYLMDSITKQMPD--GVNLYFATGRSFGDISQGLIFDDGLMLVAGFFIMFLYVSTMLGKFNLVEQRLMANKNRHCVSIISFIISYGVGSACFLVYGPVNKILPFLLLGIGVDDMFVIVQCWNNLFPDCLSKPNKLSIPERMGMALKHAGVSITVTSLTDIAAFGVGASTILPGLQSFCVYAAIGILAIFLLQSTFFVAWFALDQKRVEERRNAYCCCIQYRDDKFIPNSYSQRSYAQIFFDEYFSKYLFTPVAKTLVITVTLGCLATTIYGNTQLRQEFDPIWFLPEGSHLSQFLEKREVKYPGIGFPGFVVAHQV----NWTASFPQVEQFMHDFQGSNNIYE-FDNWYEDFKEYSNENFGTKIPEKALAPSKFKRNMGKFLYSPAGSKHRAYFKVAGNFSCGDKLPPVQMVLMPFRFKYFHGPEEHIPGMHAVKALVKNANLTFNDNSDGHMFAWSTGFVTWESDELITAELYRSVALSLACVLITIFFLLANLRMCLLCGVCVIFTIINVGGFMYFWDLTINMVSCIDIVLAIGLCVDYASHIGLAFMTCSGSRNSRAKQALRNIGPAVLNGGFSTFISISLLARSQSLAFISFYKIFTIVVGFGLFHGLIFLPVILSILGPDPYPNAFSSGTEYHFDEKGKTVEMQAISTRSIKLEKRSP 997          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1228378754|ref|XP_021950763.1| (Niemann-Pick type protein homolog 1B-like [Folsomia candida] >gi|1215270797|gb|OXA57108.1| Niemann-Pick C1 protein [Folsomia candida])

HSP 1 Score: 637.876 bits (1644), Expect = 0.000e+0
Identity = 360/993 (36.25%), Postives = 564/993 (56.80%), Query Frame = 0

Query:   66 KGRHPQPQWHLRQVNCRGGVEGS-HEWGMYVARHPILVIVVSLLITSLSGLGFLRFSSESRAEKLWIPSDSPYLENKAWLDKNYPQTRRNHLALFVAEKNILTPEGIMEMLIMHQKIHAISV-NNKTYQDLCFKLPIANIFLTKRRRRRQAEDNSVSTLNPLNSTSAIVPALASSSNDSSGAGTNSSSPSNATSSEAEESSNREEKFLFNFGDDPSD---DPQDTLSPQIYCDLVSTLDEKCLENSLLEIWRYSESRVKRLTQEEILYAVNQVAESPWFGYRTDFSVYLGA-IERNSSGHIVAAKSALYSWTAAFDPD----GELYEEGGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSP----EEQQRTLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRN--WIPSAWTQKEYGK-MFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIE--NDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNVTDWRTMVREGNFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFS------SKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGPTS-SHDEDAASTAGGSS 1032
            K +  QP      +  +G +E   + WG+ VA HP   I  + + T+L  LGF+ F+ E+R +KLWIP DS +  +  WL  N+P   R   A+F AE N+L PE +  +  +H+++  IS+ + K ++DLCFKLPI      +  + R+    SV+T  P          L +S N ++                 ++  N   +  FN    P     DP   L  + YC +V++LDE C E +  E+W ++ S +  LT+++I+  +N    SP FG + +++  LG  I  N  G I+ A++    +  A +      G+   +   G   + AD+ +  +EK   E++ + SK      G+++Y    RS+ DIT   IF D + +  G+ +MF Y  + +G+ N+VE R +++  GI  VG  + +A+G+ +   + Y P++ ILPF+ LGIGIDDMFVI+QCW NL P    ++ ++++PE++G+TMKHAGV+ITVTSLTDV AFGVGA TI+PGLQ+FC+  AI I A+++LQA++FVA  ++DQ+R+ D R+GF P I+Y    + PS W+QK Y + +F   F KI+     K +V+ +TLG  G  IYG  L++QEFDPV  LP  S+L  ++  +E ++P  G  A I     E++      E ++K S     + K    + S+++W+  FK +             +    F+ ++  FL+S  GS+++    LE    C      I      F + KF+G  EH+PG   ++N++     +       +   +S     WETDE+I+ EL++N+  + + V   T +LLAN+++C     C+  TL+++ G +HFWGLTID IS I+IVLA+GLCVDY+AHIA AF+   G R  RA  ++ S+GPA+LNGG +TFLA++LL  S+SHAF +FFK+F + + FGLFHGLV+LPV+L+L+GP   S+ +D  +  G  +
Sbjct:   10 KMQERQPILEKVSLAIQGSLENFFYRWGVLVATHPFKFIFGAFMFTALLALGFINFTQENRPDKLWIPRDSKFASDTEWLRNNFPSPLRESFAIFTAE-NVLVPEVMQAIFEIHERVGRISILDGKKWEDLCFKLPITK--FNEASKYRRRRRRSVATTLPSREV------LMTSENRTA------REEDTFFGHAFDDFDNAFNEDFFNEVSSPHVVDWDPSIELPVETYCSMVNSLDEACWEVNPTELWSFNRSLIHNLTKDDIVQVMNYQLTSPIFGRKVNYTAELGGKITHNEKGEIIGAEAIRLKFVVAINQTAVDLGKSMSDFSTG---ETADEGTWLWEKAWTELMQNASKSLPR--GIQVYYANGRSWGDITESLIFEDGMMLVVGFLVMFVYISIMIGRFNMVEQRPFLSIAGITCVGFAVIVAYGIGAMCFLLYGPVNKILPFMLLGIGIDDMFVIMQCWSNLYPSCLTKKCKQSIPERMGMTMKHAGVSITVTSLTDVAAFGVGASTIMPGLQSFCIYAAIGIIAVFLLQATFFVACFAVDQKRLEDRRNGFFPWIKYSESEFTPSEWSQKSYYQSVFEWFFFKIILTPIGKTIVILMTLGFLGVNIYGNLLLKQEFDPVWFLPDDSHLALFIKTRENNYPGTGNPAFIVA---ENVNWTASFESYDKFS----EMFKSSDEIYSFENWYDDFKIYANQHFGTDIPNKPLDPKLFQKYMGKFLYSPRGSKHRGFFKLEGNFSCGNNLPTIKMVMMPFRFRKFAGAAEHIPGMHHIKNIVASVNLTYNTGKHGRMIPWSPAMVTWETDELITEELYKNVLFSFISVLGTTFLLLANIRVCFFCGLCVIFTLINVGGMMHFWGLTIDMISFIDIVLAVGLCVDYAAHIALAFMSCSGSRAGRAEYAVRSIGPAVLNGGFSTFLAILLLANSQSHAFKSFFKIFTVVICFGLFHGLVFLPVLLTLIGPQPFSNTQDGKNEPGSGT 975          

BLAST of hypothetical protein DAPPUDRAFT_306990 vs. nr
Match: gi|1325286696|ref|XP_023328081.1| (Niemann-Pick C1 protein-like [Eurytemora affinis])

HSP 1 Score: 632.869 bits (1631), Expect = 0.000e+0
Identity = 355/982 (36.15%), Postives = 572/982 (58.25%), Query Frame = 0

Query:   91 WGMYVARHPILVIVVSLLITSLSGLGFLRFSSESRAEKLWIPSDSPYLENKAWLDKNYPQTRRNHLALFVAEKNILTPEGIMEMLIMHQK-IHAISVNNKTYQDLCFKLPIANI-----FLTKRRRRRQAEDNSVSTLNPLNSTSAIVPALASSSNDSS-GAGTNSSSPSNATSSEAEESSNREEKFLFNFGDDPSDDPQDTLSPQIYCDLVSTLDEK-CLENSLLEIW------RYSESRVKRLTQEEILYAVNQVAESPWFGYRTDFSVYLGAIERNSSGHIVAAKSALYSWTAAFDPDGELYEEGGVGVELDLADKVSLDFEKEMIEVLLDGSKESEERSGVKIYANVARSFNDITSEAIFFDAVKMAAGYFLMFFYTMLFLGKLNLVEHRTWIAAGGIISVGMGLGIAFGVTSALGMPYTPIHGILPFLALGIGIDDMFVIVQCWYNLSPEEQQR--TLPEKIGLTMKHAGVAITVTSLTDVFAFGVGAVTILPGLQAFCVTCAIAIAAIYILQASWFVALLSLDQRRIRDHRDGFAPCIRYRNWIPSAWTQKEYGKMFMAGFAKILKKGFTKIVVLGITLGVFGTGIYGTYLIRQEFDPVLLLPAQSYLRQWLAVQEVHFPTNGWGANIYTGPLEDIENDLEQFEKLSLEMDALTKDGKILKSYDSWWVPFKTFIETKRNV--TDWRTMVREG--NFEMFLSDFLFSKVGSRYKPKIILEKELVCNQPASRITATNSDFSYVKFSGPEEHVPGRRLVENLIQGKKFSSKAFSFSKVYAAWETDEVISFELWRNLALAMVCVFIITLVLLANVKICLMVLSCICLTLVDMVGFLHFWGLTIDTISCINIVLAIGLCVDYSAHIAHAFIVEEGDREERAMNSLVSMGPAILNGGITTFLALVLLGFSESHAFITFFKVFMLTVIFGLFHGLVYLPVILSLMGP----TSSHDEDAASTAGG--SSINTNSTNEDNYKPS 1046
            +G  +AR+PI  I ++L+I  L  +G LR   E+   KLWIP DS Y+ N  WL +N+P   R H  +   + N+LTPE I ++  +H+  ++  S   + + D+C+++P+ ++      L       +  ++ V  +    +T  I    + ++ DS     T  S   N+T   +  S NR ++           DP   L P+ YC L+  L    CLENSLLE+W      + ++  +  LTQE+IL A+N V  SP +    DFS YLG I RNSSGHI+ A++    +    +       E     +    D  +L +E  +I+VL   SK ++  S +    NVA+SF+D+++ AI+ DA+    G   +F Y  + LGK N V+ R  +++ G+   G+ +  ++G  S +G+ ++P+H ++PFL +G+G+DDMFVI+Q   NL  +      ++  KI  TMK  GVAIT+TS TD+ AF +GA+T+LP L++FC+ CA+ IA++Y   A+ FVA LS+D+RRIR  R+G  P I +++W P+  +Q+E+G+        +L   F +  VL  T  + G GIYG   ++QEF+P+  +P +SYL  W      +FP  G    +  G + D  N+L + E L   + AL  + +IL + DSW++ F+ F   K  V  +DW  + +E    F   L+ FLFS  G+ Y+ +     +L+C + A  +  ++ + ++  F+ P E +P    ++ L++   FSS+AF     YA+WETDEVI +EL++N+ ++++CVF+  L+L+A+++ CL+VL C+ L+LV++ G++HFWGLT+D ISC+N+ +AIGL VDYSAH+AH F++++G + ER  ++L  +GPA+LNGG +TFLA +    S+SH FI+FF++FML V+FGL+HGL++LPV L+L+GP        D+D  +T  G  S+I   ++ E+    S
Sbjct:   42 YGSILARYPIRSICLTLVIPGLCAIGLLRLEQENNPYKLWIPQDSDYIRNHEWLWQNFPDDIRFHSVILTGD-NVLTPEYIRKLYEVHEAMVNVTSSKGERWSDICYRVPMVDLEPIQDLLNDSLYEYEDYEDDVEEIP--ETTEQIQDGGSETTADSMFDITTQISVIDNSTLISSNNSENRIKR-------ASEFDPAIDLYPEDYCPLIEDLTTSICLENSLLELWGKAGYTQETKDIIYALTQEDILRAINTVTTSPVYDRAQDFSKYLGDISRNSSGHIIGARAMFLRFFGQVNTTALTDSEDVAVTKGSPVDGNTLAWEDAIIDVL---SKLADGDSSLVF--NVAKSFSDLSAGAIYGDALLFGIGTSTVFVYVQIMLGKFNFVQTRPGLSSVGLGCYGLAILTSYGFCSGIGLVFSPLHSVIPFLIMGLGVDDMFVIMQSLDNLDAKGGTALWSIERKIAETMKLGGVAITITSFTDLIAFAIGAITVLPALKSFCLFCAVGIASVYFYTATIFVAFLSIDERRIRAKRNGMCPLIVHKDWTPNKISQREFGRELFEKLGWVLSNPFVQAFVLIATAVLGGFGIYGLSELKQEFNPIKFIPPESYLAGWFHANTEYFPKEGERVIVNIGDI-DYNNELHKLENL---VGALVNETEILSAVDSWYLEFRKFAVGKNLVEESDWFNLFQENREKFYTILTQFLFSPDGAPYRRRFNFVNDLICGEAAPTVLLSSIELTHRLFTSPSEWIPAMNRIKELVREANFSSRAFPTGTEYASWETDEVIGYELYQNMGISLLCVFLTVLLLIASIRACLLVLVCVVLSLVNVAGYMHFWGLTVDVISCVNLEIAIGLTVDYSAHVAHTFLIQKGSKSERMRSTLTEIGPAVLNGGFSTFLAFIFTVGSKSHVFISFFRIFMLVVLFGLYHGLIFLPVALALIGPEPHQKRKEDDDEMNTEEGKISTIGRRASKENQVAAS 1004