AP F364 (F168+F169)

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General

End Date: 

Tuesday, April 10, 2018 - 09:00

Experiment Description: 

Mvp1 and Mvp2/3 knock-down

Experiment Batch ID: 

AP

Start Date: 

Tuesday, March 6, 2018 - 09:00

Experiment Contact: 

Christiane Eichner
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

30

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

UN53_5145, UN52_5383
LsMvp1, LsMvp2/3

Target type: 

UN53_5145
UN52_5383

Primary target ID: 

EMLSAT00000005145, EMLSAT00000005145-700992 (mRNA) Lepeophtheirus salmonis
EMLSAT00000005383, EMLSAT00000005383-701230 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Heidi Kongshaug

Fragment Label ID: 

F364

Fragment concentration: 

600.00ng/ul

Fragment length: 

576bp

Sense sequence: 

>F169 cacatggagacgaagaagtgcgattggagagagatccgtttccattatatccaggagaagagttaaaggtcaaggtcacaccattggcaatcgttcatacctcaaatgcccttttattaaaagttataagaaatttcacggacgaaaatgatgttaaacgtgtggctggagatttatttttgtttgagggacctggaacttatattccaaggaaagaggtcgaggttcttaaaaccattacagcacaagttatccatgaaaatgaagcccttaaattaagtgcaagtcgtgaaaccatagatcgatccggaattaaacacgttgcaggtgaagaatggctcataagaaagcctggagcttatttgcctttggcttatgaaaatattgtggaagttgttaaggcacatataccccttccagatgtcgcaattttggtaagagcaaaggcaactttcaaggacaagtttggtgttgagagaaaaaatggagacaaatacttgattacctttgaagatacatcagcattcattcctgaggtacaagaagaaataattggcacagtggatgctatcacac >F168 cctcccacccgaatggtcgtaatacctccaggatcttattgtgttgtatcaaaccctgttgtaacaaaagatggtactattgagaaagatcgctttggccaaattaagttatctcatggagatgaagaaattcgattaaaaagagatccttttccattatatccaggagagaaattgaaagttgaagtgacgcctttaacaatcgtacattcatcaagtgcacttcttttaaaagttatcagaaattttactgatgaagacaaaactgagcgtsttgctggagatttatacttgttcgaaggtccaggaacttattttcctagaaaagaggtggaagttgttaaaactattactgctacagtcatacatgagaatgaagctttaaaactgagtgctgctcgtgaaactttagacagatctggttgtaagagagtggcaggggagg

Fragment Mixture Formula: 

F169+F168

Fragment Producer: 

licelab

Primer Group: 

likb2502_T7_b2515
Validation

Efficacy of Knock Down: 

F>68.00%
Phenotype

Phenotype CV: 

Phenotype: 

normal

Number of Individuals (End): 

15
AttachmentSize
Image icon knock-down of LsMVP7.4 MB
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Testing the fragment for Mvp1 knock-down.