AL F338

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General

End Date: 

Friday, October 6, 2017 - 10:00

Experiment Description: 

Knockdown of the nuclear receptor Fushi tarazu factor 1 (FTZF1)

Experiment Batch ID: 

RNA_AL

Start Date: 

Monday, August 28, 2017 - 10:00

Experiment Contact: 

Joakim Brunet
Sample

Developmental Stage CV: 

PreAdult2
Adult

Number of Individuals (Start): 

30

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

FTZ-F1

Target type: 

mRNA

Primary target ID: 

EMLSAG00000008902, EMLSAG00000008902-691668 (gene) Lepeophtheirus salmonis
EMLSAG00000008900, EMLSAG00000008900-691666 (gene) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Joakim Brunet

Fragment Label ID: 

F338

Fragment Batch ID: 

F338

Fragment ID: 

FTZF1 DsRNA probe 1, FTZF1 DsRNA 1 (RNAi_reagent) Lepeophtheirus salmonis

Fragment length: 

467bp

Sense sequence: 

cgccttcatcatcgacaacaacatccatactaacctcatctacaaataaaaaacctcgttccctcaaaatcgtcgaatgtctcgactct tcctcgacgacacacaccacagcctctccatcgtcgccatcaacctcttcatggaatctc Attcttccttcgcatccttccgtagaatcaccttcatcgtcttcgagtccttctcatcaa Ggagggggagataaagagatatcctctgtggttatagcagcctctacgaccacgacagca Actaaaagatcatttagtcaacatatcatggaagaagaggaagaggacgaagacgaagaa Gaggctggaggacacttatcttggaaccctgatgaagagcaggatcaatcagctgaggag Gatgcaggactaccaacagcatccagtaacaacaactccgctggatccacatccggagacttcggtcctggagttgtg

Fragment Producer: 

licelab
Validation

Efficacy of Knock Down: 

F>45.00%
Phenotype

Phenotype CV: 

Phenotype: 

No eggstrings

Number of Individuals (End): 

12

Gallery Image: 

Image file: 

Image icon RFAL12.jpg
Image icon RFAL11_s123_1_genitalsegment_25x.jpg
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Knockdown of the nuclear FTZ-F1 in pre-adult 2. Knockdown resulted in strange genital segments. Sectioning revealed disorganization of eggs in genital segment.