AL F339

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General

End Date: 

Friday, October 6, 2017 - 00:00

Experiment Description: 

Knockdown of the nuclear receptor FTZF1

Experiment Batch ID: 

RNA_AL

Start Date: 

Monday, August 28, 2017 - 10:00

Experiment Contact: 

Joakim Brunet
Sample

Developmental Stage CV: 

PreAdult2
Adult

Number of Individuals (Start): 

30

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

FTZ-F1

Target type: 

mRNA

Primary target ID: 

EMLSAG00000008902, EMLSAG00000008902-691668 (gene) Lepeophtheirus salmonis
EMLSAG00000008900, EMLSAG00000008900-691666 (gene) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Joakim Brunet

Fragment Label ID: 

F339

Fragment Batch ID: 

F339

Fragment ID: 

FTZF1 DsRNA probe 2, FTZF1 DsRNA 2 (RNAi_reagent) Lepeophtheirus salmonis

Fragment length: 

499bp

Sense sequence: 

gtgatagggcacggaaactgcaagtcttgcgt Caaagacaagtgatacacggtggagtttccatgggcccaagtagcatgaataatacgaca Accacaacgagtagtagtgcatcctacggcggcggtgcctccggtggaatgaactccacg Agtagccctccatccatgaacaataataataataacaattccccctcaatctacggctca Cccatcactcatattaaacaagagatacaaataccacaagtcacatctatgacgtcttca Ccagatacttcaccctctcccgttcatttacccggaatgatttctaatcctggaggcaat Ccaatgggtggagatactccgggctccaatcccatcattgcccaacagactgatcctgcc Ggaggtattcaatggcagatcaatgctgctggaaagcatcaggatatccaaagtaatcaa Ggaactgggaaaatgccagccatcattcgagaattccttgcctccct

Fragment Producer: 

licelab
Validation

Efficacy of Knock Down: 

F>45.00%
Phenotype

Phenotype CV: 

Phenotype: 

No eggstrings

Number of Individuals (End): 

7

Gallery Image: 

Image file: 

Image icon RFAL14.jpg
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Knockdown of FTZF1 targetting both isoforms, alpha and beta. Only alpha is downregulated, few survivors. Sectioning reveals disorganization of eggs in genital segment, resulting in no eggstrings, same as probe F338 (other entry).