survival

Q F123

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Q

Experiment Contact: 

Heidi Kongshaug

Start Date: 

10 June, 2014 - 13:35

End Date: 

22 April, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

40

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN31_g7083
Fragment description

Fragment length: 

408bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F123

Primer Group: 

b1881

Fragment Producer: 

Licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>10%
Phenotype

Number of Individuals (End): 

13

Phenotype: 

Reduced survival
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Downregulation of gene with unknown function

Q F122

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Q

Experiment Contact: 

Heidi Kongshaug

Start Date: 

10 June, 2014 - 13:35

End Date: 

22 April, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000010654, EMLSAT00000010654-706501 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

UN30_g17511
Fragment description

Fragment length: 

370bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F122

Primer Group: 

b1879

Fragment Producer: 

Licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>30%
Phenotype

Number of Individuals (End): 

16

Phenotype: 

Reduced survival
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Q F121

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Q

Experiment Contact: 

Heidi Kongshaug

Start Date: 

10 June, 2014 - 13:35

End Date: 

22 April, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

placeholder for unknown sequence, geneofdeath (protein_coding_gene) Lepeophtheirus salmonis

Gene or target symbol: 

UN29_g5326
Fragment description

Fragment length: 

545bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F121

Primer Group: 

b1877

Fragment Producer: 

Licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>70%
Phenotype

Number of Individuals (End): 

21

Phenotype: 

Reduced survival
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Downregulation of gene with unknown function

Q F103

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Q

Experiment Contact: 

Heidi Kongshaug

Start Date: 

10 June, 2014 - 13:35

End Date: 

22 April, 2014 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

40

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000008531, EMLSAT00000008531-704378 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

UN22_g1732
Fragment description

Fragment length: 

423bp

Fragment concentration: 

600ng/ul

Fragment Label ID: 

F103

Primer Group: 

b1631

Fragment Producer: 

Licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>70%
Phenotype

Number of Individuals (End): 

18

Phenotype: 

Reduced survival
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L F102

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

L F102

Experiment Contact: 

Sussie Dalvin

Start Date: 

26 April, 2013 - 09:57

End Date: 

10 June, 2013 - 00:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

29

Developmental Stage CV: 

PreAdult
Adult
Target description

Primary target ID: 

EMLSAT00000004662, EMLSAT00000004662-700509 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

UN21_g1627
Fragment description

Fragment length: 

443bp

Fragment concentration: 

600ng/ul

Sense sequence: 

Tccgcttttttatctggaatatggcttatgtacgaagacatgaactacaatcaaggaaatccaggaggmaaagtagtttacggatggggatataatcatagcattcattataatattgacataagcaagatttcctctatgcgctacagtggaacagaatctggattacaatttgatactttgaacttttacaaagagccaggattcataggaaacgaggagatgtactatcaagacgaagcaatattgactcgacccttctttggaaaatccattattctgacaggatgcaaaccgtggactttatttaatgaaactcattttcatggtgaacatatttgtatctatccaagatctatatcatcaaataattgtactcctggtttttttgaagctccaagggattttggtgactttgtagataaggtgaggagcattcggct

Fragment Label ID: 

F102

Primer Group: 

b1629

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Validation

Efficacy of Knock Down: 

F>90%
Phenotype

Number of Individuals (End): 

4

Phenotype: 

One animal sectioned
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Few animals, one empty tank (7c) and a total of 4 animal recovered

Ovaries abnormal, eggs in oviduct abnormal. Subcuticular tissue normal.

Glandular tissue appears large ? or somehow there is less of everything else, unsure.

inconclusive in-situ

The student Julia did the qPCR validation of the sampled lice. 130927

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