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AX F418

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

AX

Experiment Contact: 

Heidi Kongshaug

Start Date: 

7 May, 2019 - 09:00

End Date: 

13 June, 2019 - 09:00

Experiment Description: 

Fragment belongs to Dylan
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

30

Developmental Stage CV: 

PreAdult2
Target description

Primary target ID: 

EMLSAT00000010925, EMLSAT00000010925-706772 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Trypsin- Dylan
Fragment description

Fragment concentration: 

600ng/ul

Fragment Batch ID: 

AX

Fragment Label ID: 

F418

Primer Group: 

likb2502_T7_b2515

Fragment Producer: 

licelab

Fragment Producer Contact: 

Heidi Kongshaug
Phenotype

Number of Individuals (End): 

3
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Testing the fragment for Mvp1 knock-down.

EMLSAT00000001458 cop + infection_b

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Zhaoran_moult nauplia + infection

Experiment Contact: 

Christiane Eichner

Start Date: 

18 June, 2018 - 14:00

End Date: 

27 June, 2018 - 10:00

Experiment Description: 

downregulation of EMLSAT00000001458 in nauplia1 and infection of salmon with copepodids
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

100

Developmental Stage CV: 

Nauplius1
Copepodid
Target description

Primary target ID: 

EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

RAB1A

Target type: 

mRNA

External DBref: 

Fragment description

Fragment length: 

412bp

Fragment concentration: 

20ng/ul

Sense sequence: 

caagctgttattgattggcgattctggtgtaggcaaatcgtgcttactattaagatttgctgatgatacttacacagaaagttacataagtacaattggtgttgactttaagattagaacaattgaactcgacggaaagactatcaagttgcaaatctgggatactgcaggccaagaaagattcagaactatcacatccagttactatcgtggtgcccacggaatcatcgttgtatatgacgttacggaccaggattcttttaataaccttaagcaatggcttcaagaaattgatagatacgcatgtgaaaatgtaaacaagcttcttgtgggaaacaaatgtgatctgacaacaaagaaggttgtagattatacaactgcaaaagaatacgctgatcagttaaatatgccg

Antisense sequence: 

Fragment Batch ID: 

Fragment Label ID: 

F366

Fragment Mixture Formula: 

Fragment Producer: 

Licelab

Fragment Producer Contact: 

Christiane Eichner
Phenotype

Number of Individuals (End): 

0

Phenotype: 

no lice survived to the end of experiment, where control lice were mainly chalimus 2
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network middle old/moult yellowgreen cluster (most preserved in moulting + chitin genes). Experiment was done again to find out when lice disappear. Lice were sampled before moulting to chalimus 1 (1 fish) and after (2 fish). There were lice on the fish before the moult but no after.

EMLSAT00000009839_Var1Frag1and2

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Exp3

Experiment Contact: 

Aina-Cathrine Øvergård
Amr Gamil

Start Date: 

11 December, 2017 - 11:17

End Date: 

18 December, 2017 - 11:47

Experiment Description: 

Knock Down in copepodids
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Developmental Stage CV: 

Nauplius1
Copepodid
Target description

Primary target ID: 

EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Heme peroxidase

Target type: 

mRNA
Fragment description

Fragment length: 

429bp

Fragment concentration: 

10ng/ul

Sense sequence: 

acctggtcagcaacgacttggcccacgagagcaagttaaccaaaatacggctttcattgatgcatctatgatttatgggaatgacaaatgtgactctattgatttacgacaaaacatcgatggtaggctgaacactacactacccagtcatggaagaggcaaacatcttatgcccaaggatagttccaataaggaatgtaaagcggcttcaggattttgtttcaaaggaggagatgctagagcctctgagcagcctggacttgctgctttacatactatttttctcagggaacataatcgtctcgtcaccaagctgaaaagagtcaatcctcattggagtgatgacgaactttattatgagggaaggcatattttatcagctgtgaatcaacacatcacttataatgagttccttcctcggatcattgg

Fragment Label ID: 

9839Fragment1
9839Fragment2

Fragment Mixture Formula: 

Fragment1 + Fragment2

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
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Image file: 

Image icon Olympys_SZX12_1.6x_31.5_ventral_.JPG

Gallery Image: 

Dead copepodid

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Testing function of vaccine candidate gene from Oslo in cooperation with Elisabeth Gislefoss!

EMLSAT00000009839_Var1Frag2

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Exp1

Experiment Contact: 

Aina-Cathrine Øvergård
Amr Gamil

Start Date: 

9 June, 2017 - 11:17

End Date: 

4 July, 2017 - 11:47

Experiment Description: 

Fish infested With KD copepodides
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

60

Developmental Stage CV: 

Copepodid
PreAdult
Target description

Primary target ID: 

EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Heme peroxidase

Target type: 

mRNA
Fragment description

Fragment length: 

266bp

Fragment concentration: 

10ng/ul

Sense sequence: 

ataatcttcgagttcaaacccaaggatattcttcagactatgaatctacctgtaatcctggcatattcaatgaatttgccacagctgccttcagatttggtcattcactcattcgtcccatgcttacccgtatgtctgctgattggaaggaaaagaaaaagcatattcgtctacgtgatggatttttcaacccagatatgttatatcaagaaacaatgattgatgaagttatcagaggtctagttgctacgcctatggagaaccag

Fragment Label ID: 

9839Fragment2

Fragment Producer: 

Aina-Cathrine Øvergård

Fragment Producer Contact: 

Aina-Cathrine Øvergård
Phenotype

Number of Individuals (End): 

3
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Testing function of vaccine candidate gene from Oslo in cooperation with Elisabeth Gislefoss!

EMLSAT00000009839_Var1Frag1

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Exp1

Experiment Contact: 

Aina-Cathrine Øvergård
Amr Gamil

Start Date: 

9 June, 2017 - 11:17

End Date: 

4 July, 2017 - 11:47

Experiment Description: 

Fish infested With KD copepodides
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

60

Developmental Stage CV: 

Copepodid
PreAdult
Target description

Primary target ID: 

EMLSAT00000009839, EMLSAT00000009839-705686 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

Heme peroxidase

Target type: 

mRNA
Fragment description

Fragment length: 

429bp

Fragment concentration: 

10ng/ul

Sense sequence: 

acctggtcagcaacgacttggcccacgagagcaagttaaccaaaatacggctttcattgatgcatctatgatttatgggaatgacaaatgtgactctattgatttacgacaaaacatcgatggtaggctgaacactacactacccagtcatggaagaggcaaacatcttatgcccaaggatagttccaataaggaatgtaaagcggcttcaggattttgtttcaaaggaggagatgctagagcctctgagcagcctggacttgctgctttacatactatttttctcagggaacataatcgtctcgtcaccaagctgaaaagagtcaatcctcattggagtgatgacgaactttattatgagggaaggcatattttatcagctgtgaatcaacacatcacttataatgagttccttcctcggatcattgg

Fragment Label ID: 

9839Fragment1
Phenotype

Number of Individuals (End): 

5
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Testing function of vaccine candidate gene from Oslo in cooperation with Elisabeth Gislefoss!

EMLSAT00000001458 cop + infection

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

network_moult nauplia + infection

Experiment Contact: 

Christiane Eichner

Start Date: 

9 May, 2018 - 09:00

End Date: 

1 June, 2018 - 10:00

Experiment Description: 

downregulation of EMLSAT00000001458 in nauplia1 and infection of salmon with copepodids
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

both

Number of Individuals (Start): 

60

Developmental Stage CV: 

Nauplius1
Copepodid
Target description

Primary target ID: 

EMLSAT00000001458, EMLSAT00000001458-697305 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

RAB1A

Target type: 

mRNA
Fragment description

Fragment length: 

412bp

Fragment concentration: 

20ng/ul

Sense sequence: 

caagctgttattgattggcgattctggtgtaggcaaatcgtgcttactattaagatttgctgatgatacttacacagaaagttacataagtacaattggtgttgactttaagattagaacaattgaactcgacggaaagactatcaagttgcaaatctgggatactgcaggccaagaaagattcagaactatcacatccagttactatcgtggtgcccacggaatcatcgttgtatatgacgttacggaccaggattcttttaataaccttaagcaatggcttcaagaaattgatagatacgcatgtgaaaatgtaaacaagcttcttgtgggaaacaaatgtgatctgacaacaaagaaggttgtagattatacaactgcaaaagaatacgctgatcagttaaatatgccg

Fragment Label ID: 

F366

Fragment Producer: 

Licelab

Fragment Producer Contact: 

Christiane Eichner
Phenotype

Number of Individuals (End): 

0

Phenotype: 

no lice survived to the end of experiment, where control lice were mainly chalimus 2
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network middle old/moult yellowgreen cluster (most preserved in moulting + chitin genes)

Functional characterization of a heme Peroxidase (male) F159

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Exp 1

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

30 May, 2014 - 12:30

End Date: 

10 June, 2014 - 12:33

Experiment Description: 

Female and male padI/padII lice were injected. Samples were taken at 6 and 10 days post injection.
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

Male

Number of Individuals (Start): 

42

Developmental Stage CV: 

padII/ad
Adult
Target description

Primary target ID: 

EMLSAT00000001069, EMLSAT00000001069-696916 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

HPX1
Fragment description

Fragment length: 

936bp

Fragment concentration: 

600ng/ul

Sense sequence: 

acttgcattacctatgaggcagtcaatgctgcatttaccactgccttagaaagagtaggacttccacctgtaaggggaaagttcactactgatgatgtgggtaatcttggaactgtgattcatgaaacgagtcgttaccttgccaaacagtatggcctatctaaagatgctatttctaatggactacctctcattgatactactaagaccatcattgaaggctactgtccaccctttttgatgactccaaagtgcacagtggagcgttacagaagtgttaacggaatgtgtaacaacgtagagcaccctcattggggagcggctatgaatggacatcacagattccttgcacctaattttgcagatggaatctctgctccaagggcctccaaaactggaaatcctcttccttctgctcgtattgtttcatctaatatgcacagagatgaaggattccacgatcatgctgtgactattcttctcgtagcctggggacagtttattgatcacgatatcactcttagtgctgaaacaaaggatcctcgtacagggaaaacacccaagtgttgtgaagagggacaaactgctgttcatcccaattgcttgccaatcgaaatcccatctcatgatacattcttctcaaaacacaagcaacgttgtatgaactttgttcgttctcaagccggtcttcgttacaattgcagactaggaccccgtgataccttcaatgaagtatcatccctcctagatgctggaaccgtgtactccaactctgatgagactcttgagagtttgagactctacaaggacggactccttaaaatgttgcccgtttttaatgaatttgaaatgaaggaactccttcctcttaaattggaatctcctgatgagggttgcattagaccttcagaagatgtgtattgtttcttggctgga

Fragment Label ID: 

F159

Fragment Producer: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>98%
Phenotype

Number of Individuals (End): 

1

Phenotype: 

Fall off the fish
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Heme peroxidases are the most abundant type of peroxidase catalyzing a H2O2-dependent oxidation of a wide variety of substrates. They are involved in numerous processes like the innate immune response, hormone and prostaglandin synthesis and crosslinking of proteins within extracellular matrixes (ECM) as well as molecules within the cuticle and chorion of arthropods and nematodes. In the present study, a Lepeophtheirus salmonis heme peroxidase (LsHPX) 1 was characterized.

Functional characterization of a heme Peroxidase (female) F159

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

Exp 1

Experiment Contact: 

Aina-Cathrine Øvergård

Start Date: 

30 May, 2014 - 12:30

End Date: 

10 June, 2014 - 12:33

Experiment Description: 

Female and male padI/padII lice were injected. Samples were taken at 6 and 10 days post injection.
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

Female

Number of Individuals (Start): 

42

Developmental Stage CV: 

PreAdult1
PreAdult2/Adult
Target description

Primary target ID: 

EMLSAT00000001069, EMLSAT00000001069-696916 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

HPX1
Fragment description

Fragment length: 

936bp

Fragment concentration: 

600ng/ul

Sense sequence: 

acttgcattacctatgaggcagtcaatgctgcatttaccactgccttagaaagagtaggacttccacctgtaaggggaaagttcactactgatgatgtgggtaatcttggaactgtgattcatgaaacgagtcgttaccttgccaaacagtatggcctatctaaagatgctatttctaatggactacctctcattgatactactaagaccatcattgaaggctactgtccaccctttttgatgactccaaagtgcacagtggagcgttacagaagtgttaacggaatgtgtaacaacgtagagcaccctcattggggagcggctatgaatggacatcacagattccttgcacctaattttgcagatggaatctctgctccaagggcctccaaaactggaaatcctcttccttctgctcgtattgtttcatctaatatgcacagagatgaaggattccacgatcatgctgtgactattcttctcgtagcctggggacagtttattgatcacgatatcactcttagtgctgaaacaaaggatcctcgtacagggaaaacacccaagtgttgtgaagagggacaaactgctgttcatcccaattgcttgccaatcgaaatcccatctcatgatacattcttctcaaaacacaagcaacgttgtatgaactttgttcgttctcaagccggtcttcgttacaattgcagactaggaccccgtgataccttcaatgaagtatcatccctcctagatgctggaaccgtgtactccaactctgatgagactcttgagagtttgagactctacaaggacggactccttaaaatgttgcccgtttttaatgaatttgaaatgaaggaactccttcctcttaaattggaatctcctgatgagggttgcattagaccttcagaagatgtgtattgtttcttggctgga

Fragment Label ID: 

F159

Fragment Producer: 

Aina-Cathrine Øvergård
Validation

Efficacy of Knock Down: 

F>78%
Phenotype

Number of Individuals (End): 

0

Phenotype: 

Fall off the fish
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Heme peroxidases are the most abundant type of peroxidase catalyzing a H2O2-dependent oxidation of a wide variety of substrates. They are involved in numerous processes like the innate immune response, hormone and prostaglandin synthesis and crosslinking of proteins within extracellular matrixes (ECM) as well as molecules within the cuticle and chorion of arthropods and nematodes. In the present study, a Lepeophtheirus salmonis heme peroxidase (LsHPX) 1 was characterized.

C F4 (CopB2)

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

C

Experiment Contact: 

Christiane Trösse

Start Date: 

14 August, 2012 - 09:00

End Date: 

24 September, 2012 - 09:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

LsCopB2
Fragment description

Fragment length: 

566bp

Fragment concentration: 

600ng/ul

Sense sequence: 

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID: 

F4

Primer Group: 

b671

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Validation

Efficacy of Knock Down: 

F>82%
Phenotype

Number of Individuals (End): 

0
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Image file: 

Image icon 4a.JPG
Image icon 4b.JPG

Gallery Image: 

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Knockdown of LsCopB2 in preadult female lice. Early termination after 15 days (Rack 4a and 4b - no survivors in 4c at normal termination date). High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

A F4 (CopB2)

Submitted by on

Phenotype CV: 

General

Experiment Batch ID: 

A

Experiment Contact: 

Christiane Trösse

Start Date: 

23 January, 2012 - 09:00

End Date: 

24 February, 2012 - 09:00
Sample

Organism: 

Lepeophtheirus salmonis

Sex: 

female

Number of Individuals (Start): 

39

Developmental Stage CV: 

PreAdult2
Adult
Target description

Primary target ID: 

EMLSAT00000000903, EMLSAT00000000903-696750 (mRNA) Lepeophtheirus salmonis

Gene or target symbol: 

LsCopB2
Fragment description

Fragment length: 

566bp

Fragment concentration: 

600ng/ul

Sense sequence: 

atcgtgtcaagtccacagacctgcacccagtggagccttggatgttggcctcgctctacaacgggaacgtgcacatttggaactacgagtctcagcagttggtcaagtccctggaggtgtgcgacttgccggtgcgagccgccaagtttgtgagccgtaagaattgggtggtgacgggttctgacgacatgaagctgaatgtgttcaactacaacaccctggagcgtgtgtcttgcctggaggcgcactctgactatctgcggtccctggccgtccacccgagtgagcccctggtcctctcctcctcggacgacatgctcattaagctctggaactgggacaaggcctgggcctgtgaacaggtcttcgagggacacacgcactacataatgcaagtggtcatcaaccccaaggacaacaacacttttgcctccgcctctttggaccgcaccatcaaggtctggcacttgggcgcatcttgccccaacttcactctggagggacatgacaagggcgtcaactgtctggactactaccacgccggggacaagccctacctcatctcg

Fragment Label ID: 

F4

Primer Group: 

b671

Fragment Producer: 

licelab

Fragment Producer Contact: 

Christiane Trösse
Validation

Efficacy of Knock Down: 

F>65%
Phenotype

Number of Individuals (End): 

5
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Image file: 

Image icon 9b.JPG
Image icon 9c.JPG

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Knockdown of LsCopB2 in preadult female lice. High mortality, empty gut, no egg strings, underdeveloped genital segment and abdomen.

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