EMLSAT00000002095_copepodites

Submitted by Aina-Cathrine Ø... on 14 June, 2018 - 13:12

Phenotype CV:

General

Experiment Batch ID:

ExpC

Experiment Contact:

Aina-Cathrine Øvergård

Start Date:

15 August, 2017 - 10:05

End Date:

21 August, 2017 - 10:09

Experiment Description:

Salmon at 12 degrees were infested in single tanks with 70 knock-down cops/fish. Sampling at 3 and 6 days post infestation. Immun gene response were measured in salmon skin at attachment site.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

both

Number of Individuals (Start):

420

Developmental Stage CV:

Copepodid

Target description

Primary target ID:

EMLSAT00000002095, EMLSAT00000002095-697942 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

Astacin

Target type:

mRNA

Fragment description

Fragment length:

267bp

Fragment concentration:

14ng/ul

Sense sequence:

aatgttatgacgactatacaaattgtcccgatttgggccatctttgtggggagaatgatggagttacagaaggctgtaaacttacttgtagattatgttaactcactacggatatatttaactcgttgctattagaatatgtaatttatatgataaatgttcccaaactatatcttaaagcattgttaaaaataagttgtaatatgaatcatacataagtcatttgatgaacttctgaatcttaaggcttaaaataaagaaatttgc

Fragment Label ID:

E2095(6965)

Fragment Producer:

Aina-Cathrine Øvergård

Fragment Producer Contact:

Aina-Cathrine Øvergård

Validation

Efficacy of Knock Down:

F>94%

Phenotype

Number of Individuals (End):

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ExpC EMLSAT00000009293

Submitted by Aina-Cathrine Ø... on 4 December, 2017 - 10:32

Phenotype CV:

General

Experiment Batch ID:

ExpC

Experiment Contact:

Aina-Cathrine Øvergård

Start Date:

15 August, 2017 - 10:05

End Date:

21 August, 2017 - 10:09

Experiment Description:

Salmon at 12 degrees were infested in single tanks with 70 knock-down cops/fish. Sampling at 3 and 6 days post infestation. Immun gene response were measured in salmon skin at attachment site.

Sample

Organism:

Lepeophtheirus salmonis

Sex:

both

Number of Individuals (Start):

420

Developmental Stage CV:

Copepodid

Target description

Primary target ID:

EMLSAT00000009293, EMLSAT00000009293-705140 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

Unknown

Target type:

mRNA

Fragment description

Fragment length:

387bp

Fragment concentration:

2000ng/ul

Sense sequence:

attcttaatgaagaatctggtgagggaaatgtcacagtcaccgtggtagatacaatggatagaagttcctttgtcatgaactccgatgatgagagtgctggtcttccagttccccttagtgaagtcgtggaaacccccaacaatgactatataatgaataatcctatcgttttaaatgagaaaaacgatgttattctgaatgacgatgttattcttagtggtgaagaccttactctgattgaaaatcccactttatcattggatgatcttctagttgaagaagaagaaagtgatgatgttattttgaactaaatgtcacaatatctttctgtatgtctaataaacagataataaatatatataacccaatgtttcattgaaaagtcc

Fragment Label ID:

E9293

Fragment Producer:

Aina-Cathrine Øvergård

Fragment Producer Contact:

Aina-Cathrine Øvergård

Validation

Efficacy of Knock Down:

F>99%

Phenotype

Number of Individuals (End):

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