EMLSAG00000000856, EMLSAG00000000856-683622 (gene) Lepeophtheirus salmonis

Overview
NameEMLSAG00000000856
Unique NameEMLSAG00000000856-683622
Typegene
OrganismLepeophtheirus salmonis (salmon louse)
Associated RNAi Experiments

Nothing found

Homology
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BLAST of EMLSAG00000000856 vs. C. finmarchicus
Match: gi|592935011|gb|GAXK01023542.1| (TSA: Calanus finmarchicus comp115244_c1_seq2 transcribed RNA sequence)

HSP 1 Score: 77.411 bits (189), Expect = 3.991e-17
Identity = 36/98 (36.73%), Postives = 62/98 (63.27%), Query Frame = 0 
Query:    3 ATRNLYLSGGFSFFALVLWLLPTFLWTGTPLPTKSKKRRESRFINIIKHVRSGSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPDLPS 100
            +TRNL+L+ GF+    +LW+LPTFL T  P   K     E R   +  ++ +G++ + ++NRL+CPQC+L ++ IN +    + +FW+ +S+ PD P+
Sbjct:  519 STRNLFLNIGFAVGGHILWILPTFLGT-LPDAEKRADDSEDRLGGLPSNL-AGADPIALWNRLLCPQCILQIVGINSINYFGKLLFWTFMSSVPDAPA 806
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BLAST of EMLSAG00000000856 vs. C. finmarchicus
Match: gi|592935012|gb|GAXK01023541.1| (TSA: Calanus finmarchicus comp115244_c1_seq1 transcribed RNA sequence)

HSP 1 Score: 77.411 bits (189), Expect = 4.264e-17
Identity = 36/98 (36.73%), Postives = 62/98 (63.27%), Query Frame = 0 
Query:    3 ATRNLYLSGGFSFFALVLWLLPTFLWTGTPLPTKSKKRRESRFINIIKHVRSGSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPDLPS 100
            +TRNL+L+ GF+    +LW+LPTFL T  P   K     E R   +  ++ +G++ + ++NRL+CPQC+L ++ IN +    + +FW+ +S+ PD P+
Sbjct:  534 STRNLFLNIGFAVGGHILWILPTFLGT-LPDAEKRADDSEDRLGGLPSNL-AGADPIALWNRLLCPQCILQIVGINSINYFGKLLFWTFMSSVPDAPA 821
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BLAST of EMLSAG00000000856 vs. C. finmarchicus
Match: gi|592930442|gb|GAXK01028103.1| (TSA: Calanus finmarchicus comp710900_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 68.1662 bits (165), Expect = 2.229e-13
Identity = 37/117 (31.62%), Postives = 66/117 (56.41%), Query Frame = 0 
Query:    3 ATRNLYLSGGFSFFALVLWLLPTFLWTGTPLPTKSKKRRESRFINIIKHVRS----------------GSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPDLPSFGV 103
            +TRNLYL+ GF+  + +LW+LPTF   GT    ++++R +S  + +   +                  G+  VD++NR + PQ +L ++AIN L  + + +FW+ +S+ PD+P+  V
Sbjct:  853 STRNLYLNIGFALGSHLLWILPTFF--GTLPEERTEERFDSESVQLGTLLDPDFEAADRLGGLPANLLGAELVDLWNRTLSPQGILQIIAINALNWLGKMVFWTFMSSVPDVPAETV 1197
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BLAST of EMLSAG00000000856 vs. C. finmarchicus
Match: gi|592943261|gb|GAXK01015292.1| (TSA: Calanus finmarchicus comp631634_c0_seq4 transcribed RNA sequence)

HSP 1 Score: 54.299 bits (129), Expect = 8.146e-9
Identity = 28/105 (26.67%), Postives = 49/105 (46.67%), Query Frame = 0 
Query:    5 RNLYLSGGFSFFALVLWLLPTFLWTGTPL--PTKSKKRRESRFINIIKHVR----------SGSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPD 97
            RNL +   F       W +P+ LW  + L   +K     ES   + +   +          + +   DIYNRL+CP C++ ++ IN +   ++ IFWS ++  P+
Sbjct:  102 RNLLVCTSFFLITSTFWFVPSLLWKKSFLLPNSKDDSTAESSLADTVHSSQERVSGIVSAIANTGVADIYNRLLCPTCIVQIIFINTMLFAAQMIFWSTITFLPN 416
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BLAST of EMLSAG00000000856 vs. C. finmarchicus
Match: gi|592943262|gb|GAXK01015291.1| (TSA: Calanus finmarchicus comp631634_c0_seq3 transcribed RNA sequence)

HSP 1 Score: 54.299 bits (129), Expect = 8.926e-9
Identity = 28/105 (26.67%), Postives = 49/105 (46.67%), Query Frame = 0 
Query:    5 RNLYLSGGFSFFALVLWLLPTFLWTGTPL--PTKSKKRRESRFINIIKHVR----------SGSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPD 97
            RNL +   F       W +P+ LW  + L   +K     ES   + +   +          + +   DIYNRL+CP C++ ++ IN +   ++ IFWS ++  P+
Sbjct:  102 RNLLVCTSFFLITSTFWFVPSLLWKKSFLLPNSKDDSTAESSLADTVHSSQERVSGIVSAIANTGVADIYNRLLCPTCIVQIIFINTMLFAAQMIFWSTITFLPN 416
[Close]
BLAST of EMLSAG00000000856 vs. C. finmarchicus
Match: gi|592943264|gb|GAXK01015289.1| (TSA: Calanus finmarchicus comp631634_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 53.9138 bits (128), Expect = 1.337e-8
Identity = 28/105 (26.67%), Postives = 49/105 (46.67%), Query Frame = 0 
Query:    5 RNLYLSGGFSFFALVLWLLPTFLWTGTPL--PTKSKKRRESRFINIIKHVR----------SGSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPD 97
            RNL +   F       W +P+ LW  + L   +K     ES   + +   +          + +   DIYNRL+CP C++ ++ IN +   ++ IFWS ++  P+
Sbjct:  102 RNLLVCTSFFLITSTFWFVPSLLWKKSFLLPNSKDDSTAESSLADTVHSSQERVSGIVSAIANTGVADIYNRLLCPTCIVQIIFINTMLFAAQMIFWSTITFLPN 416
[Close]
BLAST of EMLSAG00000000856 vs. C. finmarchicus
Match: gi|592943263|gb|GAXK01015290.1| (TSA: Calanus finmarchicus comp631634_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 53.5286 bits (127), Expect = 1.377e-8
Identity = 28/105 (26.67%), Postives = 49/105 (46.67%), Query Frame = 0 
Query:    5 RNLYLSGGFSFFALVLWLLPTFLWTGTPL--PTKSKKRRESRFINIIKHVR----------SGSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPD 97
            RNL +   F       W +P+ LW  + L   +K     ES   + +   +          + +   DIYNRL+CP C++ ++ IN +   ++ IFWS ++  P+
Sbjct:  102 RNLLVCTSFFLITSTFWFVPSLLWKKSFLLPNSKDDSTAESSLADTVHSSQERVSGIVSAIANTGVADIYNRLLCPTCIVQIIFINTMLFAAQMIFWSTITFLPN 416
[Close]
BLAST of EMLSAG00000000856 vs. C. finmarchicus
Match: gi|592943258|gb|GAXK01015295.1| (TSA: Calanus finmarchicus comp631634_c0_seq7 transcribed RNA sequence)

HSP 1 Score: 45.4394 bits (106), Expect = 1.563e-6
Identity = 19/56 (33.93%), Postives = 34/56 (60.71%), Query Frame = 0 
Query:   42 ESRFINIIKHVRSGSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPD 97
            E R   I+  + + +   DIYNRL+CP C++ ++ IN +   ++ IFWS ++  P+
Sbjct:   92 EERVSGIVSAI-ANTGVADIYNRLLCPTCIVQIIFINTMLFTAQMIFWSTITFLPN 256
[Close]
BLAST of EMLSAG00000000856 vs. C. finmarchicus
Match: gi|592943259|gb|GAXK01015294.1| (TSA: Calanus finmarchicus comp631634_c0_seq6 transcribed RNA sequence)

HSP 1 Score: 45.8246 bits (107), Expect = 2.334e-6
Identity = 19/62 (30.65%), Postives = 36/62 (58.06%), Query Frame = 0 
Query:   36 KSKKRRESRFINIIKHVRSGSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPD 97
             + +  E R   I+  + + +   DIYNRL+CP C++ ++ IN +   ++ IFWS ++  P+
Sbjct:  102 DTIQSSEERVSGIVSAI-ANTGVADIYNRLLCPTCIVQIIFINTMLFAAQMIFWSTITFLPN 284
[Close]
BLAST of EMLSAG00000000856 vs. C. finmarchicus
Match: gi|592930440|gb|GAXK01028105.1| (TSA: Calanus finmarchicus comp710900_c1_seq1 transcribed RNA sequence)

HSP 1 Score: 38.1206 bits (87), Expect = 1.457e-3
Identity = 21/43 (48.84%), Postives = 29/43 (67.44%), Query Frame = 0 
Query:    3 ATRNLYLSGGFSFFALVLWLLPTFLWTGTPLPTKSKKRRESRF 45
            +TRNLYL+ GF+  + +LW+LPTF  T   LP   ++R E RF
Sbjct:   42 STRNLYLNIGFALGSHLLWILPTFFGT---LP---EERTEERF 152
[Close]
BLAST of EMLSAG00000000856 vs. L. salmonis peptides
Match: EMLSAP00000000856 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1144:10023:12314:1 gene:EMLSAG00000000856 transcript:EMLSAT00000000856 description:"maker-LSalAtl2s1144-snap-gene-0.7")

HSP 1 Score: 231.876 bits (590), Expect = 2.040e-79
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 0 
Query:    1 MAATRNLYLSGGFSFFALVLWLLPTFLWTGTPLPTKSKKRRESRFINIIKHVRSGSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPDLPSFGVPKTNGRSLXPSK 115
            MAATRNLYLSGGFSFFALVLWLLPTFLWTGTPLPTKSKKRRESRFINIIKHVRSGSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPDLPSFGVPKTNGRSLXPSK
Sbjct:    1 MAATRNLYLSGGFSFFALVLWLLPTFLWTGTPLPTKSKKRRESRFINIIKHVRSGSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPDLPSFGVPKTNGRSLXPSK 115
[Close]
BLAST of EMLSAG00000000856 vs. L. salmonis peptides
Match: EMLSAP00000007317 (pep:novel supercontig:LSalAtl2s:LSalAtl2s411:13808:14618:-1 gene:EMLSAG00000007317 transcript:EMLSAT00000007317 description:"snap_masked-LSalAtl2s411-processed-gene-0.4")

HSP 1 Score: 117.857 bits (294), Expect = 1.345e-34
Identity = 55/59 (93.22%), Postives = 57/59 (96.61%), Query Frame = 0 
Query:    1 MAATRNLYLSGGFSFFALVLWLLPTFLWTGTPLPTKSKKRRESRFINIIKHVRSGSNAV 59
            MAATRNLYLSGGFSFFALVLWLLPTFLWTGTPLPT S K+RESRFINI+KHVRSGSNAV
Sbjct:    1 MAATRNLYLSGGFSFFALVLWLLPTFLWTGTPLPTNSDKKRESRFINIVKHVRSGSNAV 59
[Close]
BLAST of EMLSAG00000000856 vs. L. salmonis peptides
Match: EMLSAP00000009211 (pep:novel supercontig:LSalAtl2s:LSalAtl2s586:131335:134460:1 gene:EMLSAG00000009211 transcript:EMLSAT00000009211 description:"maker-LSalAtl2s586-snap-gene-1.35")

HSP 1 Score: 101.679 bits (252), Expect = 1.152e-27
Identity = 44/68 (64.71%), Postives = 54/68 (79.41%), Query Frame = 0 
Query:    2 AATRNLYLSGGFSFFALVLWLLPTFLWTGTPLPTKSKKRRESRFINIIKHVRSGSNAVDIYNRLMCPQ 69
             AT+NL +SGGF+ F L+LW++PTF W+G PLPTK+   R SR  NII HVRSG N+VDIYNRL+CPQ
Sbjct:  128 VATQNLIISGGFTLFTLLLWIIPTFFWSGRPLPTKNTDSRVSRLYNIIDHVRSGGNSVDIYNRLLCPQ 195
[Close]
BLAST of EMLSAG00000000856 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold374_size191929-processed-gene-0.18 (protein:Tk01260 transcript:snap_masked-scaffold374_size191929-processed-gene-0.18-mRNA-1 annotation:"periplasmic solute binding family protein")

HSP 1 Score: 68.9366 bits (167), Expect = 2.935e-15
Identity = 39/128 (30.47%), Postives = 62/128 (48.44%), Query Frame = 0 
Query:    5 RNLYLSGGFSFFALVLWLLPTFLWTGTPLPTKSKKRR----------------ESRFINIIKHVRSGSNAVDIYNRLMCPQCVLVVMAINFLALMSRYIFWSVLSASPDLPSFGV-----PKTNGRSL 111
            RNL ++ GF  F L++W LPTF W G+PLPT     +                ++R +    H    ++ V I+NRL+CPQC +  ++I+ L    +  +W   S+ PD   F +      +T GR +
Sbjct:  187 RNLIMNSGFLAFQLIIWFLPTFFWPGSPLPTPQNMNQTISSPGEGIGIENPLAQNR-VGAFVHNLGEASPVAIHNRLLCPQCFVQTISISTLLYAGKLFWWWWFSSIPDFKRFSIRPSPSTQTQGRHM 313
The following BLAST results are available for this feature:
BLAST of EMLSAG00000000856 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000000856 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA)
Total hits: 15
Match NameE-valueIdentityDescription
gi|592935011|gb|GAXK01023542.1|3.991e-1736.73TSA: Calanus finmarchicus comp115244_c1_seq2 trans... [more]
gi|592935012|gb|GAXK01023541.1|4.264e-1736.73TSA: Calanus finmarchicus comp115244_c1_seq1 trans... [more]
gi|592930442|gb|GAXK01028103.1|2.229e-1331.62TSA: Calanus finmarchicus comp710900_c0_seq1 trans... [more]
gi|592943261|gb|GAXK01015292.1|8.146e-926.67TSA: Calanus finmarchicus comp631634_c0_seq4 trans... [more]
gi|592943262|gb|GAXK01015291.1|8.926e-926.67TSA: Calanus finmarchicus comp631634_c0_seq3 trans... [more]
gi|592943264|gb|GAXK01015289.1|1.337e-826.67TSA: Calanus finmarchicus comp631634_c0_seq1 trans... [more]
gi|592943263|gb|GAXK01015290.1|1.377e-826.67TSA: Calanus finmarchicus comp631634_c0_seq2 trans... [more]
gi|592943258|gb|GAXK01015295.1|1.563e-633.93TSA: Calanus finmarchicus comp631634_c0_seq7 trans... [more]
gi|592943259|gb|GAXK01015294.1|2.334e-630.65TSA: Calanus finmarchicus comp631634_c0_seq6 trans... [more]
gi|592930440|gb|GAXK01028105.1|1.457e-348.84TSA: Calanus finmarchicus comp710900_c1_seq1 trans... [more]

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BLAST of EMLSAG00000000856 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self)
Total hits: 3
Match NameE-valueIdentityDescription
EMLSAP000000008562.040e-79100.00pep:novel supercontig:LSalAtl2s:LSalAtl2s1144:1002... [more]
EMLSAP000000073171.345e-3493.22pep:novel supercontig:LSalAtl2s:LSalAtl2s411:13808... [more]
EMLSAP000000092111.152e-2764.71pep:novel supercontig:LSalAtl2s:LSalAtl2s586:13133... [more]
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BLAST of EMLSAG00000000856 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000000856 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000000856 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017))
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000000856 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins)
Total hits: 1
Match NameE-valueIdentityDescription
snap_masked-scaffold374_size191929-processed-gene-0.182.935e-1530.47protein:Tk01260 transcript:snap_masked-scaffold374... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
LSalAtl2s1144supercontigLSalAtl2s1144:10023..12314 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
ensembl2013-09-26 .965016
Blast vs. GO2014-04-02
TblastN vs C. finmarchicus TSA2014-05-09
Blastp vs. self2014-05-10
Blastp vs. SwissProt2017-02-10
Blastp vs. Selected Arthropods2017-02-20
Blastp vs. NR (2/2017)2017-02-20
Blastp vs. Tigriopus kingsejongensis proteins2018-04-18
Properties
Property NameValue
Logic nameensemblgenomes
Descriptionmaker-LSalAtl2s1144-snap-gene-0.7
Biotypeprotein_coding
EvidenceIEA
NoteHypothetical protein
Cross References
External references for this gene
DatabaseAccession
Ensembl Metazoa (gene)EMLSAG00000000856 (primary cross-reference)
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
EMLSAT00000000856EMLSAT00000000856-696703Lepeophtheirus salmonismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at LSalAtl2s1144:10023..12314+

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>EMLSAG00000000856-683622 ID=EMLSAG00000000856-683622|Name=EMLSAG00000000856|organism=Lepeophtheirus salmonis|type=gene|length=2292bp|location=Sequence derived from alignment at LSalAtl2s1144:10023..12314+ (Lepeophtheirus salmonis)
TTAAGAAGTATGAGAAGAAATTGCAATTGGCAGGCAAATAGAAAGCACTA
GCATAAGATGTTGGTTGATAAGGTCGTTATGGTTCTTTCAATGTGTTGGG
TAGAGAAGGATAGTGGAATATACTAATCATTATTTCTAAACCTGTCAAAT
GAATATAAGGCATGACTTCTCAAACTGTTGGTGAGTCTTTAAAAGGAGGT
GTCAAAAACAAAAATTGCTCTTTGTTAGAATGTGGGCGCGTGTGCACGTA
GTATTGTGTCAGTCCCTATTAAGGACTAAAAACTGCAGTCTTGTCCTGGT
CGGTACTGTATATCCGTCCTAAAACTTATAGAGTTCGGTCCAGGGAAATA
AATTTATCTTCGGTTAATTCCTCGATGATGTCACTCAATTTTATTTCTTC
TTTTTTTAATCAGTTCTAGTACTGATATTCCAAAGGACTGACGGTCTTAA
TGTCTAGTCCCAATGACTAACAGGACTGTTTCTAAGACTAAACGGTACCG
AATAAATAAAGACTTTCACAGCTCTAGGTAAAAGTTGTGTCTGTACTTAT
TTAGAGTCGAGGACCGCAGTCCCGTACCACTTGTTACTCCTTAAAGAAGG
TAAAACCGATCTTTTAAGACTTCATTGAAGATTTATTTTTATTTTCTTAA
TTAATATGTTATAATTTGGAACAACATAGGTTAATGGACAACTCACTCGG
GAGAAATAATTCTCTTGAGCTCAAGGTATGTAGGTTCGATACTCGGGCAT
AATATGTTCGTTTTATATTACATCATAATGCAAAAATAAATATTTTTTTT
GCAAATATAAATGCTCTCAATATATATACATATAATATATCTCATTTGGC
TTAATAAGCCATCGACACCTTCAGGAAAAGTTCTAAGGACTGTCAGTCCT
AAGACTGTACTGAACCAAATAAATAAAGACCAACCCAAGATGATAGTCAA
AAGATAGTTTATTTTTGGGTGTGTTAGGTTGTTAACACATAATAAAGATG
TTGAATTATTCGGAAAAGAATGAAAAAATTCAATATAGAAACTGAACGAT
AATGGAATAATTTGCCTTTGTCAATTAATTTTACAAGATGGTTAACTTCC
CAACAAGTCTGATGAGATATATAACATATAGCCTTCCTCTTATATTTGTG
CCTAAAAATTCCATCCATTAATGCATCTCTCTGAACAAAATCACAATAGG
AGAATAATAATTTCTGTTCACACAATTTTTTATTTGAAAKCCACACAGAT
CAAATAGAATAAATTTAGGAAGACCAGTAGTCGATTATTGTTGATTCTTT
ACAAATCTCTAGCATGAAGTACACAAGACATTTATTTATACCAAATTATG
CAAATTTTCAATGATCATTTATATAATCACATATATATTTTTGTATTTCT
TATCGTAGTTCTTCATGACAAATATTAGTTGGATGCTTCTTCACGCTCTT
CTTTGGAGAGGAAAGGTAACGAGATCATCAGAACTGGGCTTTCCTTTCAA
TATTGTGGNCTCTTTTCTTTGGTTCACCCTCCAATGAAGGGAAAAATCCA
GATGATCGGTACACCACTATGTGAAGTTTTCATTTATTATTTTAACACTT
CTTTTAATTCAACACAGACTGTTTCTTCAATCTTCCGTGGAGGAATTAGA
TGGAAATGCACTCATTAACTTTTTTCTCAATCCAATGGCTGCAACAAGGA
ACCTCTATTTATCCGGTGGTTTTGTAAGTATCTTTTCTCCATGATGTGTT
TGTTGCAGTTGAACTCTTTGACATTTTTGACAGAGCTTTTTCGCTCTCGT
TCTCTGGTTACTTCCCACATTCTTATGGACTGGAACGCCCCTTCCCACAA
AGTCTAAGAAAAGGCGTGAGTCTCGTTTCATCAACATTATCAAGCATGTC
CGTTCTGGATCAAACGCTGTAGATATTTACAATCGTCTCATGTGTCCTCA
GTGCGTATTGGTGGTGATGGCTATCAACTTTTTGGCTGTAATATATATAT
ATATATATATATGTTTATGTTAAAATAACACTGTTGTTTTTATTAATTGT
ATCTTGATTATGCAGCTTATGTCAAGATATATCTTTTGGTCCGTTTTGTC
AGCGAGCCCAGATCTTCCAAGTTTTGGTGTGCCAAAAACCAATGGGCGTT
CTCTGAANCCATCAAAATGACTACAAATGACTTAAATTATTTGATTCATA
GCTTTAAATTTATTTTCAACTAATGAGAGAAACGAAAATAAGCAGATCCC
TATTGCCAAATATTGCCTTATTTTTTTCCTCCGAGCCTCGTG
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