EMLSAG00000004547, EMLSAG00000004547-687313 (gene) Lepeophtheirus salmonis

Overview
NameEMLSAG00000004547
Unique NameEMLSAG00000004547-687313
Typegene
OrganismLepeophtheirus salmonis (salmon louse)
Associated RNAi Experiments
Table of all RNA interference experiments conducted using this feature, grouped by Phenotype CV: female reproduction, no spermatophore
Gallery Image Title Gene or target symbol Sex/Stage Phenotype Datesort descending
AA F222 both
  1. PreAdult1 female
  2. PreAdult2 male
-Missing-
female reproduction, no spermatophore
08.09.2015 - 14:29
15.10.2015 - 00:00
Table of all RNA interference experiments conducted using this feature, grouped by Phenotype CV: -Missing-
Gallery Image Title Gene or target symbol Sex/Stage Phenotype Datesort descending
Nauplii I 12262+22100+4547+12296+12453+9341 unknown
  1. Nauplius1
-Missing-
-Missing-
01.02.2016 - 14:32
10.02.2016 - 01:36
Table of all RNA interference experiments conducted using this feature, grouped by Phenotype CV: reproduction
Gallery Image Title Gene or target symbol Sex/Stage Phenotype Datesort descending
Nauplii I 12262+22100+4547+12296+12453 unknown
  1. Nauplius I
reproductive defect as adults
reproduction
01.02.2016 - 14:42
10.02.2016 - 01:43
Homology
BLAST of EMLSAG00000004547 vs. C. finmarchicus
Match: gi|592910333|gb|GAXK01048042.1| (TSA: Calanus finmarchicus comp1254182_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 41.2022 bits (95), Expect = 7.558e-3
Identity = 47/226 (20.80%), Postives = 101/226 (44.69%), Query Frame = 0
Query:  246 SYRLLITSLSFMFVILYAYFSSVLVMLGSTKGQ------VKFNKISDFLA-DSSWRLIHIEDGITTF--KRRFYDNQAFLDTYDPKKFSVPTIMEGLEKAKNGKVAXVAYISMIQHFI--QLSYYNKVRYEEFDIIDPAIDSYYYAFPINSPLIWSFNKVMHSLRNRGIIDKIYQ------DDKTSKETIESTNVDINSIIEIYLLLMIGYMLSLIYLMLEIIFYQ 454
            S RL+    +   +I  A +++ L     T+ +      V+ +++S+ L+   S+R     +G T F  K+ F +   ++     K+F+ PT+ EG +  K G +    Y + +  ++  Q    N +    +     A+  Y  AFP  S  +  FN+ +   R  G ++++ +           ++   S  + +   +  + LL  G  LS+++L++E I+++
Sbjct: 2007 SARLMANVWALFALIFLAIYTANLAAFMITREEYFNLVGVQDSRLSNPLSLKPSFRFGTTPNGATDFVMKKNFPNMHMYM-----KEFNKPTVKEGAKAVKQGSLDAFIYDATVLEYLVGQDDECNMLTVGSWY----AMTGYGIAFPKPSKWLPFFNEQLMLYRENGDLERLQRFWFTGACKPGERKKSSSKPLALAQFMSAFFLLGCGVCLSVVFLIIEHIYFK 2657          
BLAST of EMLSAG00000004547 vs. C. finmarchicus
Match: gi|592910334|gb|GAXK01048041.1| (TSA: Calanus finmarchicus comp1254182_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 41.2022 bits (95), Expect = 7.560e-3
Identity = 47/226 (20.80%), Postives = 101/226 (44.69%), Query Frame = 0
Query:  246 SYRLLITSLSFMFVILYAYFSSVLVMLGSTKGQ------VKFNKISDFLA-DSSWRLIHIEDGITTF--KRRFYDNQAFLDTYDPKKFSVPTIMEGLEKAKNGKVAXVAYISMIQHFI--QLSYYNKVRYEEFDIIDPAIDSYYYAFPINSPLIWSFNKVMHSLRNRGIIDKIYQ------DDKTSKETIESTNVDINSIIEIYLLLMIGYMLSLIYLMLEIIFYQ 454
            S RL+    +   +I  A +++ L     T+ +      V+ +++S+ L+   S+R     +G T F  K+ F +   ++     K+F+ PT+ EG +  K G +    Y + +  ++  Q    N +    +     A+  Y  AFP  S  +  FN+ +   R  G ++++ +           ++   S  + +   +  + LL  G  LS+++L++E I+++
Sbjct: 2007 SARLMANVWALFALIFLAIYTANLAAFMITREEYFNLVGVQDSRLSNPLSLKPSFRFGTTPNGATDFVMKKNFPNMHMYM-----KEFNKPTVKEGAKAVKQGSLDAFIYDATVLEYLVGQDDECNMLTVGSWY----AMTGYGIAFPKPSKWLPFFNEQLMLYRENGDLERLQRFWFTGACKPGERKKSSSKPLALAQFMSAFFLLGCGVCLSVVFLIIEHIYFK 2657          
BLAST of EMLSAG00000004547 vs. C. finmarchicus
Match: gi|592804573|gb|GAXK01149995.1| (TSA: Calanus finmarchicus comp2730689_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 40.0466 bits (92), Expect = 1.291e-2
Identity = 20/69 (28.99%), Postives = 33/69 (47.83%), Query Frame = 0
Query:  116 TGKTIQLTDIIANILNFTYSCYMTKSTEWGFEVKNKVPVTLLEHMAYGISDFGLPILVWTEKRNKIVDF 184
            +G  I     + + LNFTY        +WG   +N     L+ H  YG +++ + +L  TE R  ++DF
Sbjct: 1043 SGYEILQLAALGDFLNFTYKTIEPPDGQWGKAGENGTWTGLIGHSLYGQTNWSMGMLSLTEDREAVIDF 1249          
BLAST of EMLSAG00000004547 vs. C. finmarchicus
Match: gi|592776839|gb|GAXK01177729.1| (TSA: Calanus finmarchicus comp6486105_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 35.4242 bits (80), Expect = 1.921e-1
Identity = 19/74 (25.68%), Postives = 37/74 (50.00%), Query Frame = 0
Query:   73 DMNGKIKKDYLNFNGIHFKTYSRPWYSYLEMNCSQDFD-SPCNCTGKTIQLTDIIANILNFTYSCYMTKSTEWG 145
            +M  K+++   N  G HF+    PW +++ +  ++     P +  G    + + +A  LNFTYS   ++  +WG
Sbjct:  260 EMENKLERRK-NLLGKHFRAVFLPWSTFIWLPDNEKASYEPDDWFGMVPDIFNSLARQLNFTYSIARSRDGQWG 478          
BLAST of EMLSAG00000004547 vs. C. finmarchicus
Match: gi|592764114|gb|GAXK01190454.1| (TSA: Calanus finmarchicus comp990749_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 35.4242 bits (80), Expect = 4.563e-1
Identity = 23/86 (26.74%), Postives = 42/86 (48.84%), Query Frame = 0
Query:  373 AIDSYYYAFPINSPLIWSFNKVMHSLRNRGIIDKIYQ------DDKTSKETIESTNVDINSIIEIYLLLMIGYMLSLIYLMLEIIF 452
            A+  Y  AFP NS     FN+++   R  G ++++ +           +E   S  + I+  +  +LLL++G + SL  L+ E I+
Sbjct:  717 AMTGYGLAFPQNSKFKTMFNEMLLEYRENGDLERLGRYWLHGVCKPNMQEKRASEPLSIDQFLSAFLLLILGTICSLFLLICEHIY 974          
BLAST of EMLSAG00000004547 vs. L. salmonis peptides
Match: EMLSAP00000004547 (pep:novel supercontig:LSalAtl2s:LSalAtl2s237:687140:697956:1 gene:EMLSAG00000004547 transcript:EMLSAT00000004547 description:"maker-LSalAtl2s237-augustus-gene-7.15")

HSP 1 Score: 941.799 bits (2433), Expect = 0.000e+0
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0
Query:    1 MESIEVFSKNRGTENLFLVQLGNVTYSNDQIRKFLSDIPIFTSFYWICIDDIVLEINLFGKYHSPIIRSVKYDMNGKIKKDYLNFNGIHFKTYSRPWYSYLEMNCSQDFDSPCNCTGKTIQLTDIIANILNFTYSCYMTKSTEWGFEVKNKVPVTLLEHMAYGISDFGLPILVWTEKRNKIVDFVKCSTFTTGAIVYNTGQQDLHSLFRAFSKKTWILLLTVLVISILFIGMVHFCRTESYFESQSYRLLITSLSFMFVILYAYFSSVLVMLGSTKGQVKFNKISDFLADSSWRLIHIEDGITTFKRRFYDNQAFLDTYDPKKFSVPTIMEGLEKAKNGKVAXVAYISMIQHFIQLSYYNKVRYEEFDIIDPAIDSYYYAFPINSPLIWSFNKVMHSLRNRGIIDKIYQDDKTSKETIESTNVDINSIIEIYLLLMIGYMLSLIYLMLEIIFYQKKLNKK 460
            MESIEVFSKNRGTENLFLVQLGNVTYSNDQIRKFLSDIPIFTSFYWICIDDIVLEINLFGKYHSPIIRSVKYDMNGKIKKDYLNFNGIHFKTYSRPWYSYLEMNCSQDFDSPCNCTGKTIQLTDIIANILNFTYSCYMTKSTEWGFEVKNKVPVTLLEHMAYGISDFGLPILVWTEKRNKIVDFVKCSTFTTGAIVYNTGQQDLHSLFRAFSKKTWILLLTVLVISILFIGMVHFCRTESYFESQSYRLLITSLSFMFVILYAYFSSVLVMLGSTKGQVKFNKISDFLADSSWRLIHIEDGITTFKRRFYDNQAFLDTYDPKKFSVPTIMEGLEKAKNGKVAXVAYISMIQHFIQLSYYNKVRYEEFDIIDPAIDSYYYAFPINSPLIWSFNKVMHSLRNRGIIDKIYQDDKTSKETIESTNVDINSIIEIYLLLMIGYMLSLIYLMLEIIFYQKKLNKK
Sbjct:    1 MESIEVFSKNRGTENLFLVQLGNVTYSNDQIRKFLSDIPIFTSFYWICIDDIVLEINLFGKYHSPIIRSVKYDMNGKIKKDYLNFNGIHFKTYSRPWYSYLEMNCSQDFDSPCNCTGKTIQLTDIIANILNFTYSCYMTKSTEWGFEVKNKVPVTLLEHMAYGISDFGLPILVWTEKRNKIVDFVKCSTFTTGAIVYNTGQQDLHSLFRAFSKKTWILLLTVLVISILFIGMVHFCRTESYFESQSYRLLITSLSFMFVILYAYFSSVLVMLGSTKGQVKFNKISDFLADSSWRLIHIEDGITTFKRRFYDNQAFLDTYDPKKFSVPTIMEGLEKAKNGKVAXVAYISMIQHFIQLSYYNKVRYEEFDIIDPAIDSYYYAFPINSPLIWSFNKVMHSLRNRGIIDKIYQDDKTSKETIESTNVDINSIIEIYLLLMIGYMLSLIYLMLEIIFYQKKLNKK 460          
BLAST of EMLSAG00000004547 vs. L. salmonis peptides
Match: EMLSAP00000012109 (pep:novel supercontig:LSalAtl2s:LSalAtl2s869:318229:331619:1 gene:EMLSAG00000012109 transcript:EMLSAT00000012109 description:"maker-LSalAtl2s869-snap-gene-2.14")

HSP 1 Score: 108.612 bits (270), Expect = 6.766e-26
Identity = 71/268 (26.49%), Postives = 134/268 (50.00%), Query Frame = 0
Query:  201 QQDLHSLFRAFSKKTWILLLTVLVISILF-IGMVHFCRTESYFESQSYRLLITSLSFMFVILYAYFSSVLVMLGSTKGQVKFNKISDFLADSSWRLIHIEDGITTFKRRFYDNQAFLDTY------DPKKFSVPTIMEGLEKAKNGKVAXVAYISMIQHFIQLSYYNKVRYEEFDIIDPAIDSYYYAFPINSPLIWSFNKVMHSLRNRGIIDKI----YQDDKTSKETIESTNVDINSIIEIYLLLMIGYMLSLIYLMLEIIFYQKKL 457
              D    FR F+   W+++ +++  SIL+ IG+    + + Y E+ S+R L+TSL++ FV+L AY+   + M  +T+  V F  I+D + +  W +++ E      + R       ++ Y      DP  F VP+IMEGL+KAK  +V     ++ + + I  S+     + +  +ID     Y+   P++SPL  SF+ +   +  +GI  ++    ++   T  + +    + I  +  ++L+L+    LSL+ L +E    Q K+
Sbjct:    5 NADYELFFRPFTDSCWVVISSIVGFSILYPIGVS--IKVKDYMETYSFRTLVTSLAYFFVLLNAYYGGAMTMFFTTEVTVPFETITDVMENPDWSVVYTEGDSFGLRSRLPRGNKDVNDYWAKVEEDPLPFVVPSIMEGLKKAKKERVVYYRDVNTVLYQISTSHDYMRDFGDLTVIDKRTIRYFCMLPLHSPLTSSFSLINIGIMEKGIRHQVFLKWFETFSTGDKEVHLIYLSIGQMSSLFLILVGAVFLSLLTLGIEFFSAQGKM 270          

HSP 2 Score: 61.6178 bits (148), Expect = 1.780e-10
Identity = 54/222 (24.32%), Postives = 98/222 (44.14%), Query Frame = 0
Query:  249 LLITSLSFMFVILYAYFSSVLVMLG----STKGQVKFNKISDFLADSSWRLIHIEDGITTFKRRFYDNQAFLDTY------DPKKFSVPTIMEGLEKAKNGKVAXVAYISMIQHFIQLSYYNKVRYEEFDIIDPAIDSYYYAFPINSPLIWSFNKVMHSLRNRGIIDKI----YQDDKTSKETIESTNVDINSIIEIYLLLMIGYMLSLIYLMLEIIFYQKK 456
            L I  +S +F+IL       L+ LG    S +G     K+ D + +  W +I+ +      + R       ++ Y      DP  F VP+IMEGL+KAK  +V     ++ + + I  S+     + +  +ID     Y    P+NSPL  SF+ +   +   GI  +     ++        ++   + I  +  ++L+L+    LSL  L +E+   ++K
Sbjct:  236 LSIGQMSSLFLILVGAVFLSLLTLGIEFFSAQG-----KMMDVMKNPDWNVIYTDGDSVPLRSRLPKGNKDVNDYWAKVEKDPSPFVVPSIMEGLKKAKKERVVYHKDVNTVLYQISTSHNYMRDFGDLTVIDKKTIRYCCMLPLNSPLTSSFSLINIGIMENGIRHQAFLTWFKAFPIGDRGVDLMYLSIGQMSSLFLILVGAVFLSLFTLGIELFSAKRK 452          
BLAST of EMLSAG00000004547 vs. Select Arthropod Genomes
Match: gb|KFM81344.1| (Glutamate receptor, ionotropic kainate 2, partial [Stegodyphus mimosarum])

HSP 1 Score: 55.0694 bits (131), Expect = 4.245e-7
Identity = 94/451 (20.84%), Postives = 170/451 (37.69%), Query Frame = 0
Query:   82 YLNFNGIHFK--TYSRPWYSYLEMNCSQDFDSPCNCTGKTIQLTDIIANILNFTYSCYMTKSTEWG-FEVKNKVPVTLLEHMAYGISDFGLPILVWTEKRNKIVDFVKCSTFTTGAIVYNTGQQDLHSLF---RAFSKKTWILLL---TVLVISILFIGMV--------HFCRTESYFESQSYRLL--------------------------ITSLSFMF--VILYAYFSSVLVMLGSTKGQVKFNKISDFLADSSWRLIHIEDGITT--FKRR----FYDNQAFLDTYDPKKFSVPTIMEGLEKAKNGKVAXVAYISMIQHFIQLSYYNKVRYEEFDIIDPAIDSYYYAFPINSPLIWSFNKVMHSLRN--RGIIDKIYQD--------------------DKTSKETIESTNVDINSIIEIYLLLMIGYMLSLIYLMLEIIFYQKKLNK 459
            Y+     HFK  T+    Y+ L    S+         G  + L   ++  L+FTY  +  K  + G ++   K    ++  +  G +D  +  L  TE R + VDF      T  +I++    Q + SLF     FS + W+L++   T + +S   +G +        H CR     E   + LL                          ITS  + F  +++ +Y +++   L   K       + D       +   + +G T+  FK      F     F+  ++ + F V +  EG+EK K G  A +   + I+       YN  R      I   +DS  Y   +         K  H LRN   G I ++ Q+                     +TSK +     + + ++  +++++++G  LS    + E  ++Q+KL +
Sbjct:  412 YMELEHSHFKVTTFLSDPYAMLR-ESSKKMTGNDRFEGYGMDLLKALSEKLHFTYEIHPVKDKKHGKYDKATKKADGMIGEIMRGEADLAIADLTITESRLEAVDFTLPFMQTGISILFKKPTQKVTSLFSFLSPFSGEVWLLVMAAYTFISLSYFLVGRLSPYEWSNPHPCRQTDLVEENVFSLLNSMWFAIGSLMQQGSDIAPTAMSTRAITSFWYFFCLIMISSYTANLAAFLTVEKLVSPIENVEDLANQEKIKYGCLANGSTSGFFKNSNMSTFQKMHKFMTKHEKEVF-VSSNTEGIEKVKKGDYAYLMEATSIE-------YNTERECNLTQIGGLLDSKGYGIAV--------KKGNHKLRNWLTGGILRLQQEGQLHTLKERWWKQKKGGGSCSQTSKSSGSVNELGLGNVGGVFVVMLLGICLSACAGVAEFFWFQRKLER 845          
The following BLAST results are available for this feature:
BLAST of EMLSAG00000004547 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000004547 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA)
Total hits: 5
Match NameE-valueIdentityDescription
gi|592910333|gb|GAXK01048042.1|7.558e-320.80TSA: Calanus finmarchicus comp1254182_c0_seq2 tran... [more]
gi|592910334|gb|GAXK01048041.1|7.560e-320.80TSA: Calanus finmarchicus comp1254182_c0_seq1 tran... [more]
gi|592804573|gb|GAXK01149995.1|1.291e-228.99TSA: Calanus finmarchicus comp2730689_c0_seq1 tran... [more]
gi|592776839|gb|GAXK01177729.1|1.921e-125.68TSA: Calanus finmarchicus comp6486105_c0_seq1 tran... [more]
gi|592764114|gb|GAXK01190454.1|4.563e-126.74TSA: Calanus finmarchicus comp990749_c0_seq1 trans... [more]
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BLAST of EMLSAG00000004547 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self)
Total hits: 2
Match NameE-valueIdentityDescription
EMLSAP000000045470.000e+0100.00pep:novel supercontig:LSalAtl2s:LSalAtl2s237:68714... [more]
EMLSAP000000121096.766e-2626.49pep:novel supercontig:LSalAtl2s:LSalAtl2s869:31822... [more]
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BLAST of EMLSAG00000004547 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000004547 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods)
Total hits: 1
Match NameE-valueIdentityDescription
gb|KFM81344.1|4.245e-720.84Glutamate receptor, ionotropic kainate 2, partial ... [more]
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BLAST of EMLSAG00000004547 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017))
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EMLSAG00000004547 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins)
Total hits: 0
Match NameE-valueIdentityDescription
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
LSalAtl2s237supercontigLSalAtl2s237:687140..697956 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
ensembl2013-09-26 .965016
Blast vs. GO2014-04-02
TblastN vs C. finmarchicus TSA2014-05-09
Blastp vs. self2014-05-10
Blastp vs. SwissProt2017-02-10
Blastp vs. Selected Arthropods2017-02-20
Blastp vs. NR (2/2017)2017-02-20
Blastp vs. Tigriopus kingsejongensis proteins2018-04-18
Properties
Property NameValue
Logic nameensemblgenomes
Descriptionmaker-LSalAtl2s237-augustus-gene-7.15
Biotypeprotein_coding
EvidenceIEA
NoteHypothetical protein
Cross References
External references for this gene
DatabaseAccession
Ensembl Metazoa (gene)EMLSAG00000004547 (primary cross-reference)
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
EMLSAT00000004547EMLSAT00000004547-700394Lepeophtheirus salmonismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at LSalAtl2s237:687140..697956+

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>EMLSAG00000004547-687313 ID=EMLSAG00000004547-687313|Name=EMLSAG00000004547|organism=Lepeophtheirus salmonis|type=gene|length=10817bp|location=Sequence derived from alignment at LSalAtl2s237:687140..697956+ (Lepeophtheirus salmonis)
TTACGATAATTATAGTAATACTTTGATTTACCTCCCAAAGTAAGGGCCTT AGTGAAAGTGAACGTGAAAATCCAATTGCTTCAAACCAAGAAAAAACCTT TGGGACACTTTCCTACTGCCATTATTTGGAAGAAATTAACAAATGAAACC ATGGAGAGTATAGAAGTATTTTCGAAAAATCGAGGTACTGAAAACTTATT CCTTGTTCAATTGGGTAACGTTACTTATTCAAACGATCAAATTCGGAAAT TTCTCTCAGATATACCTATATTCACGAGTTTTTATTGGATTTGTATAGAT GATATTGTTTTAGAAATTAATTTGTTTGGTAAATATCATTCACCGATCAT AAGGAGTGTAAAATATGATATGAATGGAAAAATAAAGAAAGACTATCTCA ATTTCAACGGTATACATTTTAAGGTAATCTGTTAGTATTTTAAACATAAA TCTACATCAAAAAACGAAATGAGTGGATTTCAAATGACAAACTGAAATTA AATTTATCATCAATGTCAATACTCACTCCAAACTAAATAAATCGATTTAA AATATAACACATTCATCTCATGATCTAAAGTACATACTAATATCAATTTT GATAGACTTACAGCCGTCCTTGGTATTCTTATTTGGAGATGAATTGTTCT CAGGATTTTGATTCCCCTTGTAATTGTACTGGCAAGACAATACAACTAAC TGATATCATTGCAAACATTTTAAATTTTACCTATTCTTGCTACATGACAA AGTCCACTGAATGGGGCTTTGAAGTCAAAAATAAAGTTCCAGTGACCCTC TTAGAACATATGGCATATGGAATATCAGATTTTGGTTTACCTATTCTCGT ATGGACGGAAAAGAGAAACAAGATCGTGGATTTTGTTAAGGTAATAGTGT GACACCTAGTTGTTTTAATTTCAGTTTTTCTGTTTTTTAAGTGTTCCACA TTCACGACTGGGGCTATAGTTTACAATACAGGACAACAGGATTTGCATTC GCTGTTCCGTGCATTTTCAAAGAAAACTTGGATATTGCTGCTTACTGTTC TTGTAATTTCAATATTGTTCATTGGAATGGTTCATTTTTGTAGAACAGAG AGCTATTTTGAAAGCCAAAGCTATAGATTATTAATTACAAGCCTGTCCTT TATGTTTGTCATATTGTATGCGTACTTTAGTAGTGTTCTAGTTATGTTAG GATCCACAAAGGGACAAGTTAAATTTAATAAAAGTAGTACATTAATTTAT CAATATTAGAATTATCTTTTAATTTTATCATATCTAAACAGTTTCAGATT TTCTGGCAGATTCTAGTTGGAGATTAATTCATATAGAAGACGGAATAACT ACATTCAAAAGAAGATTTTATGATAACCAAGCCTTTTTGGATACATATGA TCCCAAAAAATTTAGTGTTCCTACTATTATGGAAGGATTGGAAAAAGCAA AAAATGGAAAGGTTGCGMATGTCGCATATATATCAATGATTCAGTAAGGA AACTCATTCCTCGAATAATTTTTCAACAAATATGTATAAACTATTCCATT AATACTAATAGGTATGAATGTATCGTCTCTAAAGATAGGAGTTAGAATTA CTTTCCTCACTATTATTCTCCTTTGTAGAACGTCCCAATTTTCAAAAGCC CCTCTGCAAGGCATCATACTCACTAATATTATATTAAAGTATCTCAGCTA ATCAATTGAATTGTTCAGAACCATTTTAGTATCTTTCATGCTTTAGAATA ATTTACTATTATTCTTAATTGAAGTAAAACTTTTTTTAAACATTAGATCC TTGATGTTGAAGATTGATTGATGATAACGACATTCAGCAAAAATTGTTCT ATGCCACGTGATTTAGGATATCTAAATTTTAGGACTTCCAGATTCCTACT AGTCATTAAAGATAGGACAGATCACATTTTCTCGGATCCGGGGCAGTTCG GATCTTTATACCTCAATACTCGAGTACATTTTGGATACTTGAACTTTTGG ATCCAGATCCGAAACCAAAATTAGACAAATACTTGCCTTGAATTTTATTA GCATTTTAAGTAGAGACCTAAATAAAGTAGGTTTACAATTTAGATTACAT TTTTACTGGTTACTGATTCCTAAACAATTAAAAAACATGTCATTACTGTT GTTTATAATAACTTAAAAGTAAAAGCACTAATTATTGTCCTAGACTTGTC GCCACTTTCTATTAAATCACAATGCGTCTATTAACTAAAGATGTACACAC ATTACTAAAATACAGAGTTCTTTATTGCTTTAGATGAGGGGGTATGTTAT TTTCAACGTCAGTTAAAGGGAAGGAACTGCAATGTTATATTAACATTGAG GAACTGACTATTCAACTTGTGATCAGATTTGAATATACTTTCCACAGTTG GGATTAGTGATCTATATTGACAAACTTCATAATCTATATTTGTTCATAAA TTATAGCTATATACTTTTTAATAAAATGTTAAAATATGGTGATGCTTAGG TTTGCTCCAATTTGCAAAAGGGTTTGTCCTCTGGAAGTAGTTTCCATAGG TTTTATAGAGATCCAGGTGAACCTTAAATTGAATCCTAACTCCCAATTCA ATTTCAAGTTTTGTTTTCATGAATTATAATAGTCAATAGACAATATGGAA GTAAAATAAATTAAATTGATCTAAGCCAAGGTGACTGAAACAAATAATAT ATATGAAATCACGAATAAAGAAACGTCAATCCTAATGACCAGTGCCGTAT GTCAGGAAGATTTTTCGGTCCGCTTTACAAGGGTGTACGGTTGATTTTCA GTGGTGAACGTGGGATATTTAAACAGGGCAAATGCTACAGCATAGATTAA ATCTTGCGAGAATATTGTCCAATGGACTGTCTGACCATTATTTTCTTGAG GCAAACATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NTTGGAGACACCGTCCGCGGCCAGGGAGACAGTGCGGGCGGGGTTAAGGC CGAGATCTTTGTCAGCGTCGTGAAGCTTCTCAAACAAATATTTGCCGTCA GTCTGGGTAACTTTTACAAACTTGAGGAAGCTCTCTTTCTTGATGCCTTC ACTCGTCAGATATCACAGTGACAGACTGAACTGCTCCGTGTTTGACATAT CTGATGTCTCATCAACAATCACAGAGAACCAGTAGTCATCATCGCCGGTT TGTGTTTTTTGTTGTTAGAATGAACTTTCAACGAGGAACTGTTTTTCCAT GTTTTGAACTCAAATTTCCCAAGGTTGGAAATGGAAAATTTGGAMCCGGT GAAACACTTGCCTGACTTTTGAAMCTCGTCATATTCTAGCCACGGGAACT TACGGAATCAATCATAATGAAAGTCTCTGGAGTATTTGTCATCTNAGTCT TGCTCTTCTCACCTCCTTGGACATTGTCGCTAGGGAATATTGTTGTTGTT GACTACAGAGAGCTGCTCGGGAGAGAAGATTGGTCCGATATCGAGATTAG GAAGTGTCTTATTCCATGAGGAATCCGTCCGCTGGGGTAATTTGATGAGT CGCCATTATTTTCTGTGTCTGCAGTAACTTTCTCTTCAGCAGAAATGGAC TTTTCAGATCTTGGTTCAGGCAGTAAGGCCTCAACTGGAGCAGGGTCATC GGGACAGGAGGAGGCCGTGACTGAGGATGAGGATGTAGCAGAGGAAGTTT TCTTAACAGCAAAGTGCAATGTTTTCTGCTTCTTATGATCAATTTTCACT TAATACTTGCAGCTGGGGTCATTCTTTTGGAGTTTAACTTTGTGATCATT AAAGTTGTCCTTCTGTATTTCCTTCTGACAGATAGAACAAATCACCAACT CTCTGTTAAAATGCGTTTTCCGCTTCCCGTGATACATTTCTGATAATTAA TAAATTACTGCAACAATCCACTCTAAAAAGTACATAACTATTATTTATGT CCCTTTTAAGCAGTAATAATTTAATTTATCTTGTATTCAATGAAACAAAT TCTCACGCTCTTAAATATTTCAAGAGTACTCCATACGAAAAATATTATGT GCGTCTTTTTTTTTTTGGCTGTAAAGTACTTCATATTTGCAACCAGTAAC GGTAAACGTAATATCTAACTCAAATATCAACTCAATGTGAAGTACTTTAA ATCCATACGAAAAATGTTGTGTGCGTCTTTGTTTTTTATTTTTGGCTGTG AAGTACTCTTGAAATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNA TTATAATGACTCAGTACTATTATGAGTTGGTCATAAGTTACATATATATA ATAGATTTTAATAACTAATTATGACTTAATTCATCTATATTTACATACCT ATATGATTGGTGGACGGTTGAGTAAGGTTTTTTTTTTTGGTCCGCTGAAG CGGACAAAGCACAATACTGACGTACGGCCCTGGTCATGACTCTCTGTTTG AGATGATGATGATTTTTTTAAAAATTCGGAATTAGAAGAAATTAAGAATA TCGTTTTTTTACTGTTCTGGGAATACTTCTCGTTCACTTTTGTTTGAAAA TAAATAATTCAATTGCAACAATCTTTATAGCAAGATAAGCGTGTTGAGCT CCATAATAAATTACAGATCAAACAATGACCCACCTGGCAAAAGAGTATTA AAGAAATTGTCCGAATAAACTCTTGAATAAATAGTTGATATCGGAAATCT TTAAATTATTTAATTTTTATTATTAAAATATATGATTAATAATCATATAA TCATTTAATTATAGTTGATTGCTTGTTTATTTGAGTTCCATACTCTTCCT AACTCAAACTTGTGTTATTCATTAACATACTAAAGTTGCTGTTAAAATAC GAGAATGGAGAAGGTAATGATGTTCTTCTTGGGTTAATACACATAAATCC TTAGATCACCAAAACAGTTTGAACAGAACACTCCGAATCCATCTTTGCCG AGTTTAAGTTATTTTAAAGATTCCCAGACTACTCTCAGTTACCATGCCTT CGAGTCTTCCTTCTATCTTGTTCAAATTGAGCCTCATTAATGATATAGTA TCCAACATCATATCAATTCATTGAAACGATTTTATCACTTTACAGACTAA ATGAAAAATATATATACTTAATCATATAGTCATATAATCAATTTCAAACC TCAAAAACCGACTGAAATATCTGTAATAACGCTCTGGGGCCATAAGTTAG TTCTTGATTTACTTAATATACATAAAAATAAGAAATATCAGCTGATCCTT TTACATATGATATATTATCATATTTACATTGATATCTTTATGGCAAACCA TGAGAGCCGTTCAGAAACTACAGGAGGAAGTTCTTTTCCTGGATTAAAAA TATATTGTAGATGATGGTGGTCGGTCTTGATCATAAATTTTCTCCCAATG AAATATCTCTCAATTTTTTAGCAATCCAAAAATGATTACAGTTGCTTCTT TGTTAATAATTTGTTTCTGACGTAGACAATTTACGTGATGCAAAGGCAAC ATGGCGTTCCATATCTTCTTCCAGTTAGGAGAGTATCACTCCTAAACCAA TTGGAGATGCATCTATAGTTAGAAGAAGATGTAAAGAAAGATCATAATGT ATAAGACATGGTGCATTTGACACGGAACGCTTTATATTGTTAAAACTTGA TTGAGCTTGCGTGGACCTTGTAAAAATAACATTATTTTTATAAGCCATGK AAGGGGGAATCCTCTTCCGATAAGTTCTTCATAAAGTGACTATAAAGCTG TACTGATCCAAGAAAAGACTTAATGGAACTTATATGGAAAGATCGTTCAT TATGGATGTGCCTCTTCATATTAAGGTCTGTGATAGTGACTTCTNNNNNN NNNTAATTATTACCCTATACATACATTCCTCCAGATCTTCCACGGAATCA TCTTATACTTCTACATATAAGAACTAAGAAGTTCCATAAAACGATAGAAA AATCCAATGCAATACCCAGTGAGAAGTAGAGAGAGTTCTCATCCTTCATC CACAGCTCTTTCTTTGAAATTATTAAGAGTCAACTCTGCTTCGTCTCCTA CTCTAAGAAAACTATTCCATTAGTTCTTCAATGTTCTTAATGTATATAAT GCAGAAATCACTTATCTCCATCATATTTATATTCAATTCCATTTATAAGT TGGACTGCGTGATATACGGTCTATACGGATAATTATATAAGCTTCAAGCT CAAGCCTGATTTCTTTATTACTACTGAGTCACTTGCCATTGTAAAACAAG TGGAAAATGATTCTAGACTCGTAAATATTATGTGCTTCCCTTTTCTCAAT ATTTTGTCTGTAAAGTAAATTTTATTCTTCGTCACTGAATCTCAAATTGG ATGTGGACACTTTTCACGGACAATTTGGAAAATTATCTCCTCAAATTTTG CAACTTGTCGGAAGTCATGATGTTTGTCACTCCTTATAAAAACAACAGCC CCTTTTTTCAAACAGTTGTTCAAATAAAACAAAATCAATACAAACAGGAT ATACTGCATAACTGTCATTTATTGCATGTGTATGCCATCGAGGTAGGCTT CTCCCTGTAGATAACCTCCAGATTCTTTTGATAATATTCTGTTGTTACAA CTGAATGTGTGTCTTAATTGTCACAACCGTTCTTGTTTTTCTACGTTCAA TCTAATTTCGCAATCTCATTTGAAGTATAAGACTTTTTTATGCCTCAAAA ACTGTACAGCTTCATTTAACGATGATTCTTTGCCTGAGTCGTGTTCAATT TATTTGTCATTGTTTTCATCTCCACAATACAACCCAAATAAAGGTAGAGC AGAAATTGAAACATCAAGTTTACTGAAGTGCAGAAACAGTATTTTGATCC ATAAGACACATGATGTTCTCGCAACTATGAATGAACAAAGAAATAAAATA TCCTCTCTAAGTCAGTCATTTTGATGGTTGATGACTAAAAGGATATAGTT ATGCTAAAAAAACCACTTTGAAAATCAAAATTGTGGTCTTAAAATATCAA AATAGTTCTGCCAAGGCCGTATATAATGAATTGGATAAATTAATTGATGA TTACAATGCCGGAAAATTATATTCGCATGATAATTTGTGAAACAACATCA GTAAATACTGGGCTTTCAAATAAGATAGTAAAGTGTATACAAGAGATTGT TTTATGCAAAGGCATTCAGAGGCCACAATATATTGCCTGTCAACACCACA TTCTTGATAACCTTTTGAAACATACCATAAATTTCCTTATTTCCGAGCCA TCTATCAAACCTGAATTAAGTAACTCATTTATTGAAAATATATCAATGAA CTGCGAGTAACTTCGAAAAGACTATAACAGGTTAATATAGATGATGTTAA CAGAAGCAAAATCCCTGGACGGCCATTTCAAGTACTTACATGAATTATGC TAGGCTGATCGTTTTTATAAAATACTTCTCATTGTCCTCAACTCAAGTAG ATAATGCGTACAAAATTACGAAAGATAAGATGAAATTCACGTACAATGAT GTACACTTGTTGTTTTCCCCCTTTTGCCAGAAAGTTTTATTGTCTTAGTT ACAAAATGTGATTTCATCTTTAATTCTTGGGCAGATGATTAGTTTTAGAC TCAAAATTACAATTCATAAACTTTTATGGAATTGTTGATATCACATACAC GTGATACCAATAAAGAGTTTGAAAACTCATTGGAAAGGTGATAAATTGAC AATGGACATTCCCAGATTGAACCAAAATGCAGCACAAGTTCTTGAAACTT ATGGAGGAGATACATCAAAGTTCAAAAACAGAAGAATTTTTAAATTTGAA GTGTACAGGAAAAAATAAACTAAATAATATTATTTTTCTTTGTCAAAATC ATTTTTTGTATTATTTACCTTAAAATCTTTAAATTATAAAAATAAAATTA TATAAATATATACTTGAAAATTATTGATTTATACAAAACTTTACTCCACG GGAAGGAGGAAATTATCGATTAATTTTCAAAAAATACTACAAATATTTTT TAACTACTCATAAAACTTCATCATTTGTGAGTTATTTCACATTAAAAAAA ATTGTCATTACCTTACTGTACATATTCTAATCATTGAAATTATAACTGTT TTATGGTTAAACTTTCTATTTAAAAATAACTAATTATTTTTTTTGAAATG GGTAGGTATTTCAATTTACTGCATGAAGTTTATTAATATGAGAAAATTCT TTTGCATCAAGCACAAAAAAACAAACGAAACCAACGCCTTTCATTTTCAT TTAAATGTTCCCTGCTTTATTCATTGAAAGACTATCTATATGATTGTATG TGCAGTGCACTTTACTTTAATTTTGATTATAAATTAACTTATTTACTGAA ATTCTGTTCATACTTTATAGGCACTTCATCCAACTATCCTATTACAACAA AGTAAGGTATGAAGAATTTGATATCATCGATCCGGCTATTGACAGCTATT ATTATGCATTTCCAATTAATTCTCCACTAATTTGGTCATTTAATAAGGTT ATGCATTCACTTAGAAATAGAGGAATCATAGATAAGATCTATCAAGATGA TAAAACATCAAAAGAAACCATCGAATCTACAAACGTTGATATTAACAGTA TTATAGAAATATACTTATTGCTAATGATTGGTTACATGTTATCTCTAATA TATTTAATGTTGGAAATAATCTTTTATCAGAAAAAACTTAACAAAAAATA ATATGGTACGTATCAGTATAGTGTAATTACCTCAATGGCCATCGGAACGG AATACTTGGACAAATAATGGAAATTTGATAAAAAAATACACCTTTTCAAT CATTTATCACTTAAATAAAATTTGATATTATTTTTACTATGTATTTTTGG GGATATGACTTTATTTATTTAATATTGTAGAATACTATCTATATTAATAA AGCATACTGTATGTTTG
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