Mucin-like spermatophore wall protein 2 (AJ F270)

Submitted by Andreas Borchel on 26 March, 2018 - 10:39

Phenotype CV:

General

Experiment Batch ID:

AJ

Experiment Contact:

Andreas Borchel

Start Date:

21 March, 2017 - 09:08

End Date:

25 April, 2017 - 09:08

Experiment Description:

injection into young adut males, checking reproduction & spermatophore attachment of females

Sample

Organism:

Lepeophtheirus salmonis

Sex:

male

Number of Individuals (Start):

30

Developmental Stage CV:

Adult

Target description

Primary target ID:

EMLSAT00000000183, EMLSAT00000000183-696030 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

MLSWP2

Fragment description

Fragment length:

497bp

Fragment concentration:

600ng/ul

Sense sequence:

CCAGGCTTTTCACACGCTTT

Antisense sequence:

GCTCTTGGCCGAGCATAAAA

Fragment Batch ID:

F270

Fragment Label ID:

F270

Validation

Efficacy of Knock Down:

F>80%

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Control fragment used for all samples

Mucin-like spermatophore wall protein 1 (AJ F269)

Submitted by Andreas Borchel on 26 March, 2018 - 10:34

Phenotype CV:

General

Experiment Batch ID:

AJ

Experiment Contact:

Andreas Borchel

Start Date:

21 March, 2017 - 09:08

End Date:

25 April, 2017 - 09:08

Experiment Description:

injection into young adult males, checking reproduction & spermatophore attachment of females

Sample

Organism:

Lepeophtheirus salmonis

Sex:

male

Number of Individuals (Start):

30

Developmental Stage CV:

Adult

Target description

Primary target ID:

EMLSAT00000007670, EMLSAT00000007670-703517 (mRNA) Lepeophtheirus salmonis

Gene or target symbol:

MLSWP1

Fragment description

Fragment length:

527bp

Fragment concentration:

600ng/ul

Sense sequence:

TTACGTTGGCAATCGTCGTC

Antisense sequence:

GAAATCAAGTGCGGCTCCA

Fragment Batch ID:

F269

Fragment Label ID:

F269

Validation

Efficacy of Knock Down:

F>95%

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Control fragment used for all samples

AA F222

Submitted by Anna Komisarczuk on 20 October, 2015 - 14:33

Phenotype CV:

General

Experiment Batch ID:

AA

Experiment Contact:

Anna Komisarczuk

Start Date:

8 September, 2015 - 14:29

End Date:

15 October, 2015 - 00:00

Sample

Organism:

Lepeophtheirus salmonis

Sex:

both

Number of Individuals (Start):

120

Developmental Stage CV:

PreAdult1 female

PreAdult2 male

Target description

Primary target ID:

EMLSAG00000012262, EMLSAG00000012262-695028 (gene) Lepeophtheirus salmonis

EMLSAG00000004547, EMLSAG00000004547-687313 (gene) Lepeophtheirus salmonis

EMLSAG00000009341, EMLSAG00000009341-692107 (gene) Lepeophtheirus salmonis

EMLSAG00000012296, EMLSAG00000012296-695062 (gene) Lepeophtheirus salmonis

Fragment description

Fragment Label ID:

F222

Primer Group:

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No spermatophores on females. Specific? Mix of dsRNA against 4 genes.

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